Injection device of full-automatic western blot antibody incubation washing system
By designing an injection device with a fully automatic western blot antibody incubation and washing system, the problem of antibody injection relies on manual operation in the prior art is solved, fully automatic refill of antibodies is achieved, experimental efficiency and automation level are improved, and manual time and maintenance costs are reduced.
Patent Information
- Application Number
- CN202421210653.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-30
- Publication Date
- 2025-06-10
- Estimated Expiration
- 2034-05-30
AI Technical Summary
In the prior art, the injection of antibodies relies on manual manual operations, resulting in insufficient automation, wasting a lot of manual time, and reducing experimental efficiency.
An injection device with a fully automatic western blot antibody incubation and washing system is designed, including multiple secondary antibody syringes and primary antibody syringes, equipped with an injection mounting plate and mounting arm, and fully automatic refill of antibodies is achieved through secondary antibody and primary antibody injection components.
It realizes fully automatic cross-contamination-free filling of secondary and primary antibodies, saves manual time, improves experimental efficiency and intelligent automation level, and reduces device volume and maintenance costs.
Smart Images
Figure CN222965233U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of biology, in particular to an injection device of a full-automatic western blot antibody incubation and washing system. Background Art
[0002] At present, in laboratories researching proteins, the immune reaction of antibodies is a crucial link. The contact reaction between specific antibodies and a membrane support carrying the specific protein of the experimenter is a core step in the experiment process, which is of great significance for revealing the functions, structures, and interaction relationships of proteins. In the experiment, the membrane support is usually a thin film material with special properties that can stably carry the specific protein concerned by the experimenter.
[0003] However, in the prior art, a syringe is needed for antibody injection, and the antibody is currently injected manually, resulting in insufficient automation, a large amount of wasted manual time, and a reduction in the overall test efficiency. Content of the Utility Model
[0004] The purpose of the utility model is to provide an injection device of a full-automatic western blot antibody incubation and washing system, aiming to solve the technical problems in the prior art that the manual injection method for antibodies results in insufficient automation, a large amount of wasted manual time, and a reduction in the overall test efficiency.
[0005] To achieve the above purpose, an injection device of a full-automatic western blot antibody incubation and washing system adopted by the utility model includes a secondary antibody syringe, a primary antibody syringe, a secondary antibody injection assembly, a primary antibody injection assembly, an injection mounting plate, and a mounting arm. The number of the mounting arms is two, and the two mounting arms are respectively fixedly connected to the injection mounting plate and are respectively located on both sides of the injection mounting plate. The number of the secondary antibody syringes is multiple, and each secondary antibody syringe is respectively arranged on one side of the injection mounting plate. The number of the primary antibody syringes is multiple, and each primary antibody syringe is respectively arranged on one side of the injection mounting plate. The secondary antibody injection assembly is arranged on one side of the injection mounting plate and is adapted to the multiple secondary antibody syringes. The primary antibody injection assembly is arranged on one side of the injection mounting plate and is adapted to the multiple primary antibody syringes.
[0006] Wherein, the injection mounting plate includes an injection frame and an injection plate. The injection frame is fixedly connected to the injection plate and is located on one side of the injection plate. Each secondary antibody syringe and each primary antibody syringe are respectively arranged on one side of the injection frame.
[0007] Among them, the secondary antibody injection assembly includes a secondary antibody pressing plate, secondary antibody support plates, a secondary antibody lifting plate, and a secondary antibody telescopic motor. The number of the secondary antibody support plates is two. The two secondary antibody support plates respectively penetrate through the injection plate and are fixedly connected to the secondary antibody pressing plate, and are respectively located on one side of the secondary antibody pressing plate. And the secondary antibody pressing plate is arranged at the upper ends of a plurality of secondary antibody syringes. The secondary antibody lifting plate is fixedly connected to the two secondary antibody support plates and is located between the two secondary antibody support plates. The output end of the secondary antibody telescopic motor is fixedly connected to the secondary antibody lifting plate and is located at the lower end of the secondary antibody lifting plate.
[0008] Among them, the primary antibody injection assembly includes a primary antibody pressing plate, primary antibody support plates, a primary antibody lifting plate, and a primary antibody telescopic motor. The number of the primary antibody support plates is two. The two primary antibody support plates respectively penetrate through the injection plate and are fixedly connected to the primary antibody pressing plate, and are respectively located on one side of the primary antibody pressing plate. And the primary antibody pressing plate is arranged at the upper ends of a plurality of primary antibody syringes. The primary antibody lifting plate is fixedly connected to the two primary antibody support plates and is located between the two primary antibody support plates. The output end of the primary antibody telescopic motor is fixedly connected to the primary antibody lifting plate and is located at the lower end of the primary antibody lifting plate.
[0009] The beneficial effects of the injection device of the full-automatic western blot antibody incubation and washing system of the present utility model are as follows: It realizes the full-automatic cross-contamination-free injection of secondary antibodies and primary antibodies, saves a large amount of labor and time, improves the intelligence and automation of the device, improves the overall experimental efficiency and experimental progress, reduces the device volume, reduces the sample addition time, and saves the maintenance cost of the device. Description of the Drawings
[0010] In order to more clearly illustrate the technical solutions in the embodiments of the present utility model or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the drawings in the following description are only some embodiments of the present utility model. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on these drawings.
[0011] Figure 1 It is a schematic structural diagram of the injection device of the full-automatic western blot antibody incubation and washing system of the present utility model.
[0012] Figure 2 It is a top view of the injection device of the full-automatic western blot antibody incubation and washing system of the present utility model.
[0013] Figure 3 It is a rear view of the injection device of the full-automatic western blot antibody incubation and washing system of the present utility model.
[0014] 1. Secondary antibody syringe, 2. Primary antibody syringe, 3. Secondary antibody pressing plate, 4. Secondary antibody supporting plate, 5. Secondary antibody lifting plate, 6. Secondary antibody telescopic motor, 7. Primary antibody pressing plate, 8. Primary antibody supporting plate, 9. Primary antibody lifting plate, 10. Primary antibody telescopic motor, 11. Injection rack, 12. Injection plate, 13. Installation arm. Specific implementation mode
[0015] Please refer to Figures 1 to 3 , the present utility model provides an injection device for a full-automatic western blot antibody incubation and washing system, including a secondary antibody syringe 1, a primary antibody syringe 2, a secondary antibody injection assembly, a primary antibody injection assembly, an injection mounting plate and an installation arm 13. The number of the installation arms 13 is two, and the two installation arms 13 are respectively fixedly connected to the injection mounting plate and are respectively located on both sides of the injection mounting plate. The number of the secondary antibody syringes 1 is multiple, and each secondary antibody syringe 1 is respectively arranged on one side of the injection mounting plate. The number of the primary antibody syringes 2 is multiple, and each primary antibody syringe 2 is respectively arranged on one side of the injection mounting plate. The secondary antibody injection assembly is arranged on one side of the injection mounting plate and is adapted to the multiple secondary antibody syringes 1. The primary antibody injection assembly is arranged on one side of the injection mounting plate and is adapted to the multiple primary antibody syringes 2.
[0016] Further, the injection mounting plate includes an injection rack 11 and an injection plate 12. The injection rack 11 is fixedly connected to the injection plate 12 and is located on one side of the injection plate 12. Each secondary antibody syringe 1 and each primary antibody syringe 2 are respectively arranged on one side of the injection rack 11.
[0017] Further, the secondary antibody injection assembly includes a secondary antibody pressing plate 3, a secondary antibody supporting plate 4, a secondary antibody lifting plate 5 and a secondary antibody telescopic motor 6. The number of the secondary antibody supporting plates 4 is two. The two secondary antibody supporting plates 4 respectively penetrate through the injection plate 12 and are fixedly connected to the secondary antibody pressing plate 3 and are respectively located on one side of the secondary antibody pressing plate 3. And the secondary antibody pressing plate 3 is arranged at the upper end of the multiple secondary antibody syringes 1. The secondary antibody lifting plate 5 is fixedly connected to the two secondary antibody supporting plates 4 and is located between the two secondary antibody supporting plates 4. The output end of the secondary antibody telescopic motor 6 is fixedly connected to the secondary antibody lifting plate 5 and is located at the lower end of the secondary antibody lifting plate 5.
[0018] Further, the primary antibody injection assembly includes a primary antibody pressure plate 7, two primary antibody support plates 8, a primary antibody lifting plate 9, and a primary antibody telescopic motor 10. The number of the primary antibody support plates 8 is two. The two primary antibody support plates 8 respectively penetrate through the injection plate 12 and are fixedly connected to the primary antibody pressure plate 7, and are respectively located on one side of the primary antibody pressure plate 7. The primary antibody pressure plate 7 is disposed at the upper ends of a plurality of the primary antibody syringes 2. The primary antibody lifting plate 9 is fixedly connected to the two primary antibody support plates 8 and is located between the two primary antibody support plates 8. The output end of the primary antibody telescopic motor 10 is fixedly connected to the primary antibody lifting plate 9 and is located at the lower end of the primary antibody lifting plate 9.
[0019] In this embodiment, the injection plate 12 mounts and fixes the injection rack 11, and sequentially places the secondary antibody syringe 1 and the primary antibody syringe 2 that have already aspirated the antibody on the upper end of the injection rack 11, and are located at the lower ends of the corresponding secondary antibody pressure plate 3 and the primary antibody pressure plate 7. The mounting arm 13 is used to mount the injection plate 12. When performing primary antibody injection, the primary antibody telescopic motor 10 is driven to contract, driving the primary antibody lifting plate 9 to descend, and thereby driving the primary antibody support plate 8 and the primary antibody pressure plate 7 to press down. The primary antibody pressure plate 7 contacts a plurality of the primary antibody syringes 2, and injects the primary antibody inside each primary antibody syringe 2 into the incubation box, realizing the full-automatic filling of the secondary antibody and the primary antibody. When performing secondary antibody injection, the secondary antibody telescopic motor 6 is driven to contract, driving the secondary antibody lifting plate 5 to descend, and thereby driving the secondary antibody support plate 4 and the secondary antibody pressure plate 3 to press down. The secondary antibody pressure plate 3 contacts a plurality of the secondary antibody syringes 1, and injects the secondary antibody inside each secondary antibody syringe 1 into the incubation box, improving the intelligence and automation of the device, improving the overall experimental efficiency and experimental progress, reducing the device volume, reducing the sample addition time, and saving the maintenance cost of the device.
[0020] The above-disclosed is only a preferred embodiment of the present invention, and of course, it cannot be used to limit the scope of the rights of the present invention. Those of ordinary skill in the art can understand the entire or partial processes of implementing the above embodiments, and the equivalent changes made according to the claims of the present invention still fall within the scope covered by the present invention.
Claims
1. An injection device for a fully automatic western blot antibody incubation and washing system, characterized in that: It includes a secondary antibody syringe, a primary antibody syringe, a secondary antibody injection assembly, a primary antibody injection assembly, an injection mounting plate and a mounting arm. There are two mounting arms, and the two mounting arms are respectively fixedly connected to the injection mounting plate and are respectively located on both sides of the injection mounting plate. There are multiple secondary antibody syringes, and each of the secondary antibody syringes is respectively arranged on one side of the injection mounting plate. There are multiple primary antibody syringes, and each of the primary antibody syringes is respectively arranged on one side of the injection mounting plate. The secondary antibody injection assembly is arranged on one side of the injection mounting plate and is compatible with the multiple secondary antibody syringes. The primary antibody injection assembly is arranged on one side of the injection mounting plate and is compatible with the multiple primary antibody syringes.
2. The injection device of the fully automatic western blot antibody incubation and washing system according to claim 1, characterized in that: The injection mounting plate includes an injection frame and an injection plate. The injection frame is fixedly connected to the injection plate and is located on one side of the injection plate. Each of the secondary antibody syringes and each of the primary antibody syringes are respectively arranged on one side of the injection frame.
3. The injection device of the fully automatic western blot antibody incubation and washing system as claimed in claim 2, characterized in that: The secondary antibody injection assembly includes a secondary antibody pressure plate, a secondary antibody support plate, a secondary antibody lifting plate and a secondary antibody telescopic motor. There are two secondary antibody support plates. The two secondary antibody support plates respectively penetrate the injection plate and are fixedly connected to the secondary antibody pressure plate, and are respectively located on one side of the secondary antibody pressure plate. The secondary antibody pressure plate is arranged at the upper end of multiple secondary antibody syringes. The secondary antibody lifting plate is fixedly connected to the two secondary antibody support plates and is located between the two secondary antibody support plates. The output end of the secondary antibody telescopic motor is fixedly connected to the secondary antibody lifting plate and is located at the lower end of the secondary antibody lifting plate.
4. The injection device of the fully automatic western blot antibody incubation and washing system as claimed in claim 3, characterized in that: The anti-injection assembly includes an anti-pressure plate, an anti-support plate, an anti-lifting plate and an anti-telescopic motor. There are two anti-support plates. The two anti-support plates respectively penetrate the injection plate and are fixedly connected to the anti-pressure plate, and are respectively located on one side of the anti-pressure plate. The anti-pressure plate is arranged at the upper end of a plurality of anti-injectors. The anti-lifting plate is fixedly connected to the two anti-support plates and is located between the two anti-support plates. The output end of the anti-telescopic motor is fixedly connected to the anti-lifting plate and is located at the lower end of the anti-lifting plate.