Immunochromatography detection device for preset sample

Abstract

The utility model provides an immunochromatography detection device of preset sample, including detachable setting detection casing and preset component, the surface of detection casing is provided with the sample window, the sample window is provided with at least one puncture piece, the preset component includes first sealing piece, second sealing piece and preset cavity, the first sealing piece is provided with the puncture piece, the second sealing piece is provided with the puncture piece, and the preset cavity is provided with the puncture piece. The preset cavity is used for packaging a sample to be detected; the first sealing piece and the second sealing piece are arranged at the two opposite ends of the preset cavity respectively for sealing, the preset cavity is provided with a limiting protrusion, the end, provided with the limiting protrusion, of the preset cavity extends into the sample window, the puncturing piece punctures the first sealing piece, and the second sealing piece punctures the second sealing piece. A to-be-detected sample enters the detection shell. The utility model has the advantages of high sensitivity and good repeatability, and the packaged sample to be detected can meet the requirement of normal temperature storage and transportation.

Description

Technical Field

[0001] The utility model belongs to the technical field of immunochromatographic detection, and relates to an immunochromatographic detection device with a preset sample. Background Technique

[0002] Immunochromatographic technology is a simple and rapid diagnostic method and an important tool for point-of-care testing (POCT). It was first used for the detection of human chorionic gonadotropin (HCG) as a diagnostic basis for early pregnancy. Immunochromatographic technology is not only simple to operate, rapid in detection, strong in specificity, good in stability, but also convenient to carry, and has been widely used in the fields of clinical diagnosis, food safety, drug detection, environmental pollution, etc.

[0003] Immunochromatographic technology is mainly completed by means of an immunochromatographic test strip card. There are mainly two types of test strip cards: semi-solid phase reagent cards and solid phase reagent cards. Among them, for the semi-solid phase products, since the sample is first added to the liquid containing the fluorescent antibody conjugate for homogeneous reaction, the product has good repeatability, but the liquid containing the fluorescent antibody conjugate must be stored refrigerated. For the solid phase reagent card, the fluorescent antibody conjugate is first freeze-dried into balls, and the sample solution is injected during use to dissolve the lyophilizer. It can be stored at room temperature and reused, but the structure is complex and the manufacturing cost is high, increasing the detection cost.

[0004] However, during the dilution process of the current immunochromatographic reagent card, the sample not only has the risk of being contaminated, but also the concentration of the target analyte in the sample will be reduced, resulting in inaccurate chromatographic detection results. Therefore, it is very important to provide a detection device with accurate detection results and avoiding sample contamination. Summary of the Utility Model

[0005] Aiming at the deficiencies of the existing technology, the purpose of the utility model is to provide an immunochromatographic detection device with a preset sample. By presetting the sample to be detected, the detection sensitivity is improved, the repeatability is good, the packaged sample to be detected can meet the requirements of room temperature storage and transportation, the structure is simple, and the operation is convenient.

[0006] To achieve this purpose, the utility model adopts the following technical solutions:

[0007] The utility model provides an immunochromatographic detection device with a preset sample. The immunochromatographic detection device with a preset sample includes a detachable detection housing and a preset component. A sample window is provided on the surface of the detection housing, and at least one puncture member is provided in the sample window. The preset component includes a first seal, a second seal and a preset cavity for encapsulating the sample to be detected;

[0008] The first seal and the second seal are respectively arranged at opposite ends of the preset cavity for sealing. The preset cavity is provided with a limiting protrusion. The preset cavity extends into the sample window from the end provided with the limiting protrusion. The piercing member pierces the first seal so that the sample to be tested enters the detection housing.

[0009] In the utility model, the sample to be tested is pre - placed in the preset cavity for sealing, and then the preset cavity is loaded on the detection housing. The piercing member is used to pierce the seal to release the sample to be tested in the preset cavity into the detection housing for detection, which is convenient for scanning and analyzing the fluorescence signal on the detection window, avoiding sample contamination, preventing the sample from being affected by moisture and affecting the detection result, having good repeatability. The encapsulated sample to be tested can meet the requirements of normal - temperature storage and transportation, and is easy to operate.

[0010] The immunochromatographic detection device for preset samples provided by the utility model has a wide range of applications, and is particularly suitable for the detection of freeze - dried samples.

[0011] It should be noted that the utility model does not make specific limitations or special requirements for the sample to be tested. Those skilled in the art can, according to the actual situation, encapsulate freeze - dried balls or solutions into the preset cavity.

[0012] As a preferred technical solution of the utility model, the detection housing includes an upper cover body and a lower cover body that are buckled with each other. The upper cover body and the lower cover body are detachably connected. The sample window is opened on the surface of the upper cover body, and a test strip is detachably arranged on the lower cover body.

[0013] To help those skilled in the art better understand the overall technical solution and working process of the utility model, the utility model exemplarily provides the following specific structure for the detachable connection between the upper cover body and the lower cover body: A plurality of protrusion parts are arranged along the inner edge of the upper cover body, and grooves corresponding to the protrusion parts one by one are arranged along the inner edge of the lower cover body. The protrusion parts are pressed into the grooves to realize the clamping connection between the upper cover body and the lower cover body.

[0014] It should be noted that the above description of the connection structure between the upper cover body and the lower cover body does not further limit the protection scope of the utility model. That is to say, other connection structures that can realize the detachable connection between the upper cover body and the lower cover body also fall within the protection scope and the disclosure scope of the utility model. Therefore, other forms of connection structures that have been disclosed in the prior art or not disclosed in new technologies can also be used in the utility model.

[0015] As a preferred technical solution of the utility model, the limiting protrusion includes a locking section and a positioning section arranged in sequence from top to bottom. The diameter of the positioning section is smaller than that of the locking section. The positioning section is used for being clamped in the sample window, and the locking section is screwed into the sample window to fix the preset cavity on the detection housing.

[0016] It should be noted that the locking section and the positioning section are arranged in sequence from top to bottom as described in the present utility model, which means that the locking section and the positioning section are arranged in sequence from the end face of the preset cavity to the side away from the preset cavity.

[0017] As a preferred technical solution of the present utility model, the outer surface of the locking section is provided with threads, and the positioning section has a smooth outer surface.

[0018] In the present utility model, the diameter of the positioning section is smaller than that of the locking section. During use, the positioning section first enters the sample window for positioning, and then the locking section is gradually screwed into the sample window until the preset cavity is fixed on the detection housing.

[0019] As a preferred technical solution of the present utility model, the inner cavity surface of the sample window has a threaded section, the puncture member is located below the threaded section, and the threaded section is engaged with the locking section.

[0020] As a preferred technical solution of the present utility model, the puncture member includes a pointed end portion and a connecting portion. The connecting portion is fixed on the inner cavity surface of the sample window, and the pointed end portion extends towards the side close to the preset cavity.

[0021] The pointed end portion includes a needle shape, a sheet shape, a rectangular tooth shape, a triangular tooth shape or a cone shape.

[0022] As a preferred technical solution of the present utility model, the number of the puncture members is at least two, and at least two puncture members are evenly distributed along the inner cavity surface of the sample window.

[0023] As a preferred technical solution of the present utility model, the first sealing member and the second sealing member are aluminum foils.

[0024] In the present utility model, first, the first sealing member is used to seal one end of the preset cavity. After adding freeze-dried balls or directly adding samples from the other end of the preset cavity and then freezing and storing, after finishing adding freeze-dried balls or adding samples and freezing, the second sealing member is used for sealing.

[0025] As a preferred technical solution of the present utility model, an observation window is further provided on the surface of the upper cover body, and the observation window is used to observe the result of the test strip fixed on the lower cover body.

[0026] As a preferred technical solution of the present utility model, a card slot is provided on the inner surface of the lower cover body, and the test strip is fixed in the card slot.

[0027] Exemplarily, the detection method of an immunochromatographic detection device for preset samples provided by the present utility model is as follows:

[0028] (1) Fix the test strip in the card slot on the surface of the lower cover body, and then snap the upper cover body onto the lower cover body to complete the assembly of the detection housing;

[0029] (2) Heat-seal the bottom of the pre-set cavity with the first sealing member, put the freeze-dried balls into the pre-set cavity, and heat-seal the top of the pre-set cavity with the second sealing member to complete the encapsulation of the sample;

[0030] (3) Snap the positioning section of the limiting protrusion into the sample window of the upper cover body without damaging the first sealing member;

[0031] (4) Open the second sealing member and inject buffer solution into the pre-set cavity;

[0032] (5) Continue to screw the locking section of the limiting protrusion into the sample window, and the locking section meshes with the threaded section of the sample window for fixation. The piercing member pierces the first sealing member, releases the sample into the detection housing, and falls onto the test strip on the lower cover body for detection;

[0033] (6) Display the detection result through the observation window opened on the surface of the upper cover body.

[0034] Compared with the prior art, the beneficial effects of the present utility model are as follows:

[0035] The present utility model provides an immunochromatographic detection device with pre-set samples. The sample to be detected is pre-put into the pre-set cavity for sealing, and then the pre-set cavity is loaded on the detection housing. The piercing member is used to pierce the sealing member, and the sample to be detected in the pre-set cavity is released into the detection housing for detection, which is convenient for scanning and analyzing the fluorescence signal on the detection window, avoiding sample contamination, preventing the sample from being affected by moisture and affecting the detection result, having good repeatability, and the encapsulated sample to be detected can meet the requirements of normal temperature storage and transportation, and is easy to operate. Description of the Drawings

[0036] Figure 1 It is a schematic structural diagram of the detection housing before loading the pre-set component provided by a specific embodiment of the present utility model;

[0037] Figure 2 It is a schematic structural diagram of the detection housing after loading the pre-set component provided by a specific embodiment of the present utility model;

[0038] Figure 3 It is a schematic structural diagram of the upper cover body provided by a specific embodiment of the present utility model;

[0039] Figure 4 It is a schematic structural diagram of the lower cover body provided by a specific embodiment of the present utility model.

[0040] Among them, 1 - detection housing; 2 - preset cavity; 3 - first seal; 4 - second seal; 5 - limit projection; 51 - locking section; 52 - positioning section; 6 - upper cover body; 7 - sample window; 8 - observation window; 9 - lower cover body; 10 - test strip. Detailed implementation mode

[0041] It should be understood that in the description of the present invention, the orientation or positional relationship indicated by terms such as "center", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, so it cannot be understood as a limitation to the present invention. In addition, terms such as "first" and "second" are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the quantity of the indicated technical features. Thus, features defined with "first", "second", etc. may explicitly or implicitly include one or more of such features. In the description of the present invention, unless otherwise specified, the meaning of "a plurality" is two or more.

[0042] It should be noted that in the description of the present invention, unless otherwise clearly specified and limited, the terms "set", "connected", "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected, or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood through specific situations.

[0043] The technical solution of the present invention will be further described below with reference to the drawings and through specific implementation modes.

[0044] In some implementation modes, the present invention provides an immunochromatographic detection device for preset samples, as Figure 1 , Figure 2 and Figure 3 shown, which includes a detection housing 1 and a preset assembly that are detachably arranged. A sample window 7 is opened on the surface of the detection housing 1, and at least one puncture member is arranged in the sample window 7. The preset assembly includes a first seal 3, a second seal 4 and a preset cavity 2, and the preset cavity 2 is used for encapsulating the sample to be detected.

[0045] The first seal 3 and the second seal 4 are respectively arranged at opposite ends of the preset cavity 2 for sealing. The preset cavity 2 is provided with a limiting protrusion 5. The preset cavity 2 extends into the sample window 7 from the end provided with the limiting protrusion 5. The piercing member pierces the first seal 3, enabling the sample to be tested to enter the detection housing 1.

[0046] In the present utility model, the sample to be tested is pre - placed in the preset cavity 2 for sealing, and then the preset cavity 2 is loaded onto the detection housing 1. A piercing member is used to pierce the seal, releasing the sample to be tested in the preset cavity 2 into the detection housing 1 for detection. This facilitates the scanning and analysis of the fluorescence signal on the detection window, avoids contaminating the sample, prevents the sample from being affected by moisture and influencing the detection result, has good repeatability, and the encapsulated sample to be tested can meet the requirements of normal - temperature storage and transportation, with convenient operation. The immunochromatographic detection device for preset samples provided by the present utility model has a wide range of applications, and is particularly suitable for the detection of freeze - dried samples.

[0047] In some embodiments, the detection housing 1 includes an upper cover body 6 and a lower cover body 9 that are snap - fitted with each other. The upper cover body and the lower cover body are detachably connected. The sample window 7 is opened on the surface of the upper cover body, and a test strip 10 is detachably arranged on the lower cover body.

[0048] To help those skilled in the art better understand the overall technical solution and working process of the present utility model, the present utility model exemplarily provides the following specific structure for the detachable connection between the upper cover body and the lower cover body: A plurality of protrusion parts are arranged along the inner edge of the upper cover body 6, and grooves corresponding to the protrusion parts one by one are arranged along the inner edge of the lower cover body 9. The protrusion parts are pressed into the grooves to achieve the snap - connection between the upper cover body 6 and the lower cover body 9.

[0049] In some embodiments, the limiting protrusion 5 includes a locking section 51 and a positioning section 52 arranged in sequence from top to bottom. The diameter of the positioning section 52 is smaller than that of the locking section 51. The positioning section 52 is used for being clamped in the sample window 7, and the locking section 51 is screwed into the sample window 7 to fix the preset cavity 2 on the detection housing. In the present utility model, the locking section 51 and the positioning section 52 arranged in sequence from top to bottom mean that the locking section 51 and the positioning section 52 are arranged in sequence from the end face of the preset cavity 2 towards the side away from the preset cavity 2.

[0050] In some embodiments, the outer surface of the locking section 51 is provided with threads, and the positioning section 52 has a smooth outer surface. In the present utility model, the diameter of the positioning section 52 is smaller than that of the locking section 51. During use, the positioning section 52 first enters the sample window 7 for positioning, and then the locking section 51 is gradually screwed into the sample window 7 until the preset cavity 2 is fixed on the detection housing 1.

[0051] In some embodiments, the inner cavity surface of the sample window 7 has a threaded section, the piercing member is located below the threaded section, and the threaded section meshes with the locking section 51.

[0052] In some embodiments, the piercing member includes a pointed end portion and a connecting portion. The connecting portion is fixed to the inner cavity surface of the sample window 7, and the pointed end portion extends toward the side close to the preset cavity 2. The pointed end portion includes a needle shape, a sheet shape, a rectangular tooth shape, a triangular tooth shape, or a cone shape.

[0053] In some embodiments, the number of the piercing members is at least two, and at least two of the piercing members are evenly distributed along the inner cavity surface of the sample window 7.

[0054] In some embodiments, the first sealing member 3 and the second sealing member 4 are aluminum foils. In the present utility model, first, the first sealing member 3 is used to thermally seal one end of the preset cavity 2. After adding freeze-dried balls or directly adding samples from the other end of the preset cavity 2 and then storing them frozen, after finishing adding the freeze-dried balls or adding samples and freezing, the second sealing member 4 is used for thermal sealing.

[0055] In some embodiments, an observation window 8 is further provided on the surface of the upper cover body 6, and the observation window 8 is used to observe the result of the test strip 10 fixed to the lower cover body 9.

[0056] In some embodiments, as Figure 4 shown, a clamping groove is provided on the inner surface of the lower cover body 9, and the test strip 10 is fixed in the clamping groove.

[0057] Exemplarily, a detection method of an immunochromatographic detection device for preset samples provided by the present utility model is as follows:

[0058] (1) Fix the test strip 10 in the clamping groove on the surface of the lower cover body 9, and then snap the upper cover body 6 onto the lower cover body 9 to complete the assembly of the detection housing 1;

[0059] (2) Use the first sealing member 3 to thermally seal the bottom of the preset cavity 2, and put freeze-dried balls into the preset cavity 2;

[0060] (3) Use the second sealing member 4 to thermally seal the top of the preset cavity 2 to complete the encapsulation of the sample;

[0061] (4) Snap the positioning section 52 of the limiting protrusion 5 into the sample window 7 of the upper cover body 6 without damaging the first sealing member 3;

[0062] (5) Open the second sealing member 4 and inject buffer solution into the preset cavity 2;

[0063] (6)Continue to screw the locking section 51 of the limit projection 5 into the sample window 7. The locking section 51 meshes with the threaded section of the sample window 7 for fixation. The piercing member pierces the first seal 3, releases the sample into the detection housing 1, and falls onto the test strip 10 on the lower cover 9 for detection;

[0064] (7)The detection result is displayed through the observation window 8 opened on the surface of the upper cover 6.

[0065] The applicant declares that the above description is only the specific implementation manner of the present utility model, but the protection scope of the present utility model is not limited thereto. Those skilled in the art should understand that any changes or substitutions that can be easily thought of by those skilled in the art within the technical scope disclosed by the present utility model fall within the protection scope and the disclosure scope of the present utility model.

Claims

1. An immunochromatographic detection device with a preset sample, characterized in that: The immunochromatographic detection device for presetting samples comprises a detachable detection housing and a presetting component, a sample window is provided on the surface of the detection housing, at least one puncture member is provided in the sample window, the presetting component comprises a first sealing member, a second sealing member and a presetting cavity, and the presetting cavity is used to encapsulate the sample to be detected; The first sealing member and the second sealing member are respectively arranged at opposite ends of the preset cavity for sealing. The preset cavity is provided with a limiting protrusion. The preset cavity extends into the sample window from one end provided with the limiting protrusion. The piercing member pierces the first sealing member, so that the sample to be tested enters the detection shell.

2. The immunochromatographic detection device with a preset sample according to claim 1, characterized in that: The detection housing comprises an upper cover body and a lower cover body which are buckled with each other, the upper cover body and the lower cover body are detachably connected, the sample window is opened on the surface of the upper cover body, and a detection test paper is detachably provided on the lower cover body.

3. The immunochromatographic detection device with a preset sample according to claim 1, characterized in that: The limiting protrusion includes a locking section and a positioning section arranged in sequence from top to bottom, the diameter of the positioning section is smaller than the diameter of the locking section, the positioning section is used to be clamped in the sample window, and the locking section is screwed into the sample window to fix the preset cavity on the detection shell.

4. The immunochromatographic detection device with a preset sample according to claim 3, characterized in that: The outer surface of the locking section is provided with threads, and the positioning section has a smooth outer surface.

5. The immunochromatographic detection device with a preset sample according to claim 4, characterized in that: The inner cavity surface of the sample window has a threaded section, the puncture member is located below the threaded section, and the threaded section is meshed with the locking section.

6. The immunochromatographic detection device with a preset sample according to claim 1, characterized in that: The puncture member includes a tip portion and a connection portion, wherein the connection portion is fixed to the inner cavity surface of the sample window, and the tip portion extends toward a side close to the preset cavity; The tip portion includes a needle shape, a sheet shape, a rectangular tooth shape, a triangular tooth shape or a cone shape.

7. The immunochromatographic detection device with a pre-set sample according to claim 6, characterized in that: The number of the puncture members is at least two, and the at least two puncture members are evenly distributed along the inner cavity surface of the sample window.

8. The immunochromatographic detection device with a preset sample according to claim 1, characterized in that: The first sealing member and the second sealing member are aluminum foils.

9. The immunochromatographic detection device with a pre-set sample according to claim 2, characterized in that: The surface of the upper cover body is also provided with an observation window, and the observation window is used to observe the result of the test paper fixed to the lower cover body.

10. The immunochromatographic detection device with a preset sample according to claim 9, characterized in that: The inner surface of the lower cover body is provided with a card slot, and the test paper is fixed in the card slot.

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