Magnetic nanoparticle solid-phase extraction column for pesticide residue detection
By designing a magnetic nanoparticle solid-phase extraction column including a fixed seat, a piston, an extraction column body, an installation tube, a heating plate, a pressure relief hole, a pressure relief box, a pressure relief plate and a blocking ball, the problems of inconvenient operation and easy breakage of the extraction column in the prior art are solved, and efficient extraction and safe operation are achieved.
Patent Information
- Application Number
- CN202421670474.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-15
- Publication Date
- 2025-06-13
- Estimated Expiration
- 2034-07-15
AI Technical Summary
The existing magnetic nanoparticle solid-phase extraction columns need to manually move the extraction column or replace the collection container when collecting liquids, which is inconvenient to operate and easily break the inner wall of the solid-phase extraction column due to excessive pressure.
A magnetic nanoparticle solid-phase extraction column including a fixed seat, a piston, an extraction column body, an installation tube, a heating plate, a pressure relief hole, a pressure relief box, a pressure relief plate and a blocking ball were designed. Through the combination of these structures, an automated liquid collection and pressure release are achieved, and the inner wall of the extraction column is broken.
It improves the extraction rate and working efficiency, simplifies the operation process, avoids the risk of crushing the inner wall of the extraction column, and enhances the safety and practicality of the device.
Smart Images
Figure CN222969244U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of solid-phase extraction, in particular to a magnetic nanoparticle solid-phase extraction column for pesticide residue detection. Background Art
[0002] Solid-phase extraction is a sample pretreatment technology developed in the mid-1980s, mainly used for sample separation, purification and enrichment. The main purpose is to reduce the interference of the sample matrix and improve the detection sensitivity.
[0003] When performing solid-phase extraction, after loading the sample, it is usually necessary to wash the stationary phase to wash away the unwanted sample components. At this time, it is necessary to collect and process the liquid after washing. After washing, it is necessary to elute the analyte from the stationary phase. At this time, it is necessary to separately collect the liquid after elution.
[0004] However, the existing magnetic nanoparticle solid-phase extraction column device requires washing and elution, and at the same time, it is necessary to separately collect the liquid after washing and elution. Therefore, two sets of containers are required for the collection work. When in use, it is necessary to manually move the extraction column or replace the collection container, which is relatively inconvenient to operate, has low practicability, and squeezes the liquid in the extraction column out of the extraction column by pressing the syringe. However, the inner wall of the solid-phase extraction column has limited pressure resistance. When the pressure is accidentally too high, the inner wall of the solid-phase extraction column may be broken. Therefore, a magnetic nanoparticle solid-phase extraction column for pesticide residue detection is provided to solve the problems raised in the above background art. Summary of the Utility Model
[0005] In order to make up for the above deficiencies, the utility model provides a magnetic nanoparticle solid-phase extraction column for pesticide residue detection, aiming to improve the problems in the prior art that when collecting, it is necessary to manually move the extraction column or replace the collection container, which is relatively inconvenient to operate, has low practicability, and squeezes the liquid in the extraction column out of the extraction column by pressing the syringe. However, the inner wall of the solid-phase extraction column has limited pressure resistance. When the pressure is accidentally too high, the inner wall of the solid-phase extraction column may be broken.
[0006] To achieve the above object, the present utility model provides the following technical solutions: A magnetic nanoparticle solid-phase extraction column for pesticide residue detection, comprising a fixed seat. Four support columns are fixedly connected to the upper part of the fixed seat. A fixed plate is fixedly connected to the upper part of the support columns. A number of extraction column bodies are fixedly arranged inside the fixed plate. A feed port is opened at the upper part of the extraction column body. A piston is arranged at the upper part of the extraction column body. A packing adsorption layer is fixedly installed in the middle side inside the extraction column body. An upper sieve plate is fixedly arranged above the packing adsorption layer. A lower sieve plate is fixedly arranged below the packing adsorption layer. An installation pipe is fixedly installed at the middle side outside the extraction column body. A heating plate is fixedly arranged on the left side inside the installation pipe. A refrigeration plate is fixedly installed on the right side inside the installation pipe. A pressure relief box is fixedly installed at the lower right side of the extraction column body. A pressure relief hole is opened inside the pressure relief box. Drainage ports are opened on both sides inside the pressure relief box. A spring is fixedly installed on the inner bottom plate of the pressure relief box. A pressure relief plate is fixedly installed above the spring. A blocking ball is fixedly installed above the pressure relief plate. A collection component is arranged inside the fixed seat, and the collection component is used for collecting the solution of the accessories inside the fixed plate.
[0007] Further, the collection component includes two installation plates. The installation plates are arranged inside the fixed seat. A collection box is arranged inside the installation plates. A number of collection pipes are fixedly arranged inside the collection box. A slider is fixedly connected to the lower part of the installation plate. A chute is opened inside the fixed seat. A motor is fixedly installed at the lower part of the right installation plate. The output end of the motor is fixedly connected to a first gear. A second gear is rotatably connected to the lower part of the left installation plate. A toothed plate is fixedly connected to the middle side inside the fixed seat. A stop block is fixedly installed on the inner wall of the fixed seat.
[0008] Further, the first gear and the second gear are meshed with the toothed plate.
[0009] Further, the slider is slidably connected inside the chute.
[0010] Further, a discharge port is arranged at the lower part of the extraction column body.
[0011] Further, a sealing ring is arranged outside the blocking ball.
[0012] Further, the blocking ball is slidably connected inside the pressure relief box.
[0013] Further, the installation plate is slidably connected inside the fixed seat.
[0014] The present utility model has the following beneficial effects:
[0015] 1. In the present utility model, through the cooperation among structures such as a fixed seat, a piston, an extraction column body, a mounting pipe, a heating plate, a pressure relief hole, a pressure relief box, a pressure relief plate, and a plug ball, the extraction liquid is introduced into the interior of the column through a feed port, and then into the interior of the upper sieve plate. Then, the extract in the extraction liquid is adsorbed by a packing adsorption layer, and the remaining extraction liquid enters the interior of the lower sieve plate. According to the melting point of the extract, the extract is heated or cooled, and then the extract adsorbed on the packing adsorption layer is heated or cooled by a heating plate or a cooling plate inside the mounting pipe, so that extracts with different melting points are separated from the packing adsorption layer, making the extraction faster, thus improving the extraction rate and the working efficiency. When a corresponding external syringe is inserted into the extraction column body and squeezed, if the pressure is accidentally too high, the excessive pressure will cause the plug ball to move, driving the pressure relief plate to slide inside the pressure relief box and compress the spring. Then, the sample liquid will flow into the pressure relief box through the gap generated between the plug ball and the pressure relief hole and be discharged through the drain port, thus reminding the staff to stop pressurizing in time to avoid damage to the inner wall of the column.
[0016] 2. In the present utility model, through the cooperation among structures such as a mounting plate, a collecting pipe, a fixed seat, a motor, a second gear, a first gear, a toothed plate, and a stop block, by starting the motor, the output end of the motor drives the first gear to rotate, thereby driving the second gear to rotate. Since the first gear and the second gear are meshed with the toothed plate, the mounting plate is driven to slide. At the same time, the slider slides and is limited inside the chute, so that the stop block slides and is limited inside the mounting plate, making the collecting pipe inside the collecting box aligned with the discharge port of the extraction column body, facilitating the collection of the solution. BRIEF DESCRIPTION OF THE DRAWINGS
[0017] Figure 1 is a three-dimensional view of the magnetic nanoparticle solid-phase extraction column for pesticide residue detection proposed by the present utility model;
[0018] Figure 2 is a schematic diagram of the internal structure of the fixed seat of the magnetic nanoparticle solid-phase extraction column for pesticide residue detection proposed by the present utility model;
[0019] Figure 3 is a schematic diagram of the lower part of the mounting plate of the magnetic nanoparticle solid-phase extraction column for pesticide residue detection proposed by the present utility model;
[0020] Figure 4 is a schematic diagram of the internal structure of the extraction column body of the magnetic nanoparticle solid-phase extraction column for pesticide residue detection proposed by the present utility model;
[0021] Figure 5 is a schematic diagram of the internal structure of the pressure relief box of the magnetic nanoparticle solid-phase extraction column for pesticide residue detection proposed by the present utility model.
[0022] Legend Explanation:
[0023] 1. Extraction column body; 2. Fixed seat; 3. Collection tube; 4. Collection box; 5. Installation plate; 6. Slide block; 7. Chute; 8. Motor; 9. First gear; 10. Rack; 11. Support column; 12. Fixed plate; 13. Upper sieve plate; 14. Lower sieve plate; 15. Packing adsorption layer; 16. Piston; 17. Heating plate; 18. Refrigeration plate; 19. Feed inlet; 20. Discharge outlet; 21. Sealing ring; 22. Blocking ball; 23. Pressure relief hole; 24. Drainage port; 25. Spring; 26. Pressure relief plate; 27. Pressure relief box; 28. Installation pipe; 29. Second gear; 30. Stop block. Specific embodiments
[0024] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.
[0025] Referring to Figure 1 , Figure 4 and Figure 5 , an embodiment provided by the present invention: A magnetic nanoparticle solid-phase extraction column for pesticide residue detection, including a fixed seat 2. Four support columns 11 are fixedly connected to the upper part of the fixed seat 2. The upper part of the support column 11 is fixedly connected to a fixed plate 12. A plurality of extraction column bodies 1 are fixedly arranged inside the fixed plate 12. A feed inlet 19 is opened at the upper part of the extraction column body 1. A piston 16 is arranged at the upper part of the extraction column body 1. A packing adsorption layer 15 is fixedly installed in the middle of the extraction column body 1. An upper sieve plate 13 is fixedly arranged above the packing adsorption layer 15. A lower sieve plate 14 is fixedly arranged below the packing adsorption layer 15. An installation pipe 28 is fixedly installed in the middle of the outside of the extraction column body 1. A heating plate 17 is fixedly arranged on the left side inside the installation pipe 28. A refrigeration plate 18 is fixedly installed on the right side inside the installation pipe 28. A pressure relief box 27 is fixedly installed on the lower right side of the extraction column body 1. A pressure relief hole 23 is opened inside the pressure relief box 27. Drainage ports 24 are opened on both sides inside the pressure relief box 27. A spring 25 is fixedly installed on the inner bottom plate of the pressure relief box 27. A pressure relief plate 26 is fixedly installed on the upper part of the spring 25. A blocking ball 22 is fixedly installed on the upper part of the pressure relief plate 26. A collection assembly is arranged inside the fixed seat 2, and the collection assembly is used to collect the solution of the accessories inside the fixed plate 12;
[0026] By opening the piston 16 on the feed inlet 19, the extractant is introduced into the interior of the column body from the feed inlet 19, then into the interior of the upper sieve plate 13, and then the extract in the extractant is adsorbed by the packing adsorption layer 15. Magnetic nanoparticles are added to the interior of the packing adsorption layer 15. Then the remaining extractant enters the interior of the lower sieve plate 14. According to the melting point of the extract, the extract is heated or cooled. Then, the extract adsorbed on the packing adsorption layer 15 is heated or cooled by the heating plate 17 or the cooling plate 18 inside the installation pipe 28, so that the extracts with different melting points are separated from the packing adsorption layer 15, making the extraction faster, thus improving the extraction rate and the working efficiency. When the corresponding external syringe is inserted into the extraction column body 1 and squeezed, if the pressure is accidentally too high, the excessive pressure will cause the blocking ball 22 to move, driving the pressure relief plate 26 to slide inside the pressure relief box 27 and compress the spring 25. Then the sample liquid will flow into the pressure relief box 27 through the gap generated by the blocking ball 22 and the pressure relief hole 23 and be discharged through the drain port 24, thus reminding the staff to stop pressurizing in time to avoid damage to the inner wall of the column body. At the same time, when the pressure disappears or does not reach a certain value, under the elastic force of the spring 25, the pressure relief plate 26 slides to drive the blocking ball 22 to block the pressure relief hole 23.
[0027] Refer to Figure 1 , Figure 2 and Figure 3 , the collection assembly includes two mounting plates 5. The mounting plates 5 are arranged inside the fixed seat 2. A collection box 4 is arranged inside the mounting plates 5. A number of collection tubes 3 are fixedly arranged inside the collection box 4. A slider 6 is fixedly connected to the lower part of the mounting plate 5. A chute 7 is opened inside the fixed seat 2. A motor 8 is fixedly installed at the lower part of the right mounting plate 5. The output end of the motor 8 is fixedly connected to a first gear 9. The lower part of the left mounting plate 5 is rotatably connected to a second gear 29. A toothed plate 10 is fixedly connected to the middle side inside the fixed seat 2. A stop block 30 is fixedly installed on the inner wall of the fixed seat 2;
[0028] By starting the motor 8, the output end of the motor 8 drives the first gear 9 to rotate, thereby driving the second gear 29 to rotate. Since the first gear 9 and the second gear 29 are meshed with the toothed plate 10, the mounting plate 5 is driven to slide. At the same time, the slider 6 slides and is limited inside the chute 7, and the stop block 30 slides and is limited inside the mounting plate 5, so that the collection tubes 3 inside the collection box 4 are aligned with the discharge port 20 of the extraction column body 1.
[0029] Refer to Figure 1 , Figure 3 and Figure 4, the first gear 9 and the second gear 29 are meshed and connected with the toothed plate 10. The slider 6 is slidably connected inside the chute 7. A discharge port 20 is provided at the lower part of the extraction column body 1. A sealing ring 21 is provided outside the plug ball 22. The plug ball 22 is slidably connected inside the pressure relief box 27. The mounting plate 5 is slidably connected inside the fixed seat 2;
[0030] Through the meshing connection of the first gear 9 and the second gear 29 with the toothed plate 10, the mounting plate 5 can be driven to slide, so that the collection pipe 3 inside the collection box 4 is aligned with the discharge port 20 of the extraction column body 1. By sliding the slider 6 inside the chute 7, the sliding of the mounting plate 5 can be limited. Through the discharge port 20, the liquid inside the extraction column body 1 can be conveniently discharged. The sealing ring 21 improves the sealing performance between the plug ball 22 and the pressure relief hole 23 to avoid leakage. By sliding the plug ball 22 inside the pressure relief box 27, the staff can be reminded to stop pressurizing in time to avoid damage to the inner wall of the column. By sliding the mounting plate 5 inside the fixed seat 2, the mounting plate 5 can be slid to align the collection pipe 3 inside the collection box 4 with the discharge port 20 of the extraction column body 1 for collection.
[0031] Working principle: When using this device, by opening the piston 16 on the feed port 19, the extraction liquid is introduced into the inside of the column from the feed port 19. Then the extraction liquid enters the inside of the upper sieve plate 13, and then the extract in the extraction liquid is adsorbed by the packing adsorption layer 15. Then the remaining extraction liquid enters the inside of the lower sieve plate 14. According to the melting point of the extract, the extract is heated or cooled. Then the heating plate 17 or the cooling plate 18 inside the installation pipe 28 is used to heat or cool the extract adsorbed on the packing adsorption layer 15, so that the extracts with different melting points are separated from the packing adsorption layer 15, making the extraction faster. After completion, by starting the motor 8, the motor 8 drives the first gear 9 to rotate, which drives the second gear 29 to rotate. Since the first gear 9 and the second gear 29 are meshed and connected with the toothed plate 10, the mounting plate 5 is driven to slide. At the same time, the slider 6 slides and limits the position inside the chute 7, so that the stopper 30 slides and limits the position inside the mounting plate 5, making the collection pipe 3 inside the collection box 4 aligned with the discharge port 20 of the extraction column body 1. Then an external corresponding syringe is inserted into the extraction column body 1 and squeezed. When the pressure is accidentally too high, the excessive pressure will cause the plug ball 22 to move, driving the pressure relief plate 26 to slide and compress the spring 25 inside the pressure relief box 27. Then the sample liquid will flow into the pressure relief box 27 through the gap generated between the plug ball 22 and the pressure relief hole 23 and be discharged through the drain port 24, thereby reminding the staff to stop pressurizing in time to avoid damage to the inner wall of the column. At the same time, when the pressure disappears or does not reach a certain value, under the elastic force of the spring 25, the pressure relief plate 26 slides to drive the plug ball 22 to block the pressure relief hole 23, and the sealing ring 21 is used to further improve the sealing performance between the two to avoid leakage.
[0032] Finally, it should be noted that the above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.
Claims
1. A magnetic nanoparticle solid phase extraction column for pesticide residue detection, comprising a fixing seat (2), characterized in that: Four support columns (11) are fixedly connected to the upper part of the fixed seat (2), a fixed plate (12) is fixedly connected to the upper part of the support column (11), a plurality of extraction column bodies (1) are fixedly arranged inside the fixed plate (12), a feed inlet (19) is opened at the upper part of the extraction column body (1), a piston (16) is arranged at the upper part of the extraction column body (1), a packing adsorption layer (15) is fixedly installed on the middle side of the inside of the extraction column body (1), an upper sieve plate (13) is fixedly arranged on the upper part of the packing adsorption layer (15), a lower sieve plate (14) is fixedly arranged on the lower part of the packing adsorption layer (15), a mounting pipe (28) is fixedly installed on the middle side of the outside of the extraction column body (1), and the mounting A heating plate (17) is fixedly installed on the left side of the interior of the tube (28), a cooling plate (18) is fixedly installed on the right side of the interior of the installation tube (28), a pressure relief box (27) is fixedly installed on the right side of the lower part of the extraction column body (1), a pressure relief hole (23) is opened inside the pressure relief box (27), and drainage ports (24) are opened on both sides of the interior of the pressure relief box (27), a spring (25) is fixedly installed on the bottom plate of the pressure relief box (27), a pressure relief plate (26) is fixedly installed on the upper part of the spring (25), and a blocking ball (22) is fixedly installed on the upper part of the pressure relief plate (26), and a collecting component is arranged inside the fixing seat (2), and the collecting component is used to collect the solution of the internal accessories of the fixing plate (12).
2. The magnetic nanoparticle solid phase extraction column for pesticide residue detection according to claim 1, characterized in that: The collecting assembly comprises two mounting plates (5), the mounting plates (5) being arranged inside the fixing seat (2), the mounting plates (5) being provided with a collecting box (4), the collecting box (4) being fixedly provided with a plurality of collecting tubes (3), the lower part of the mounting plates (5) being fixedly connected with a sliding block (6), the fixing seat (2) being provided with a sliding groove (7), the lower part of the right mounting plate (5) being fixedly installed with a motor (8), the output end of the motor (8) being fixedly connected with a gear one (9), the lower part of the left mounting plate (5) being rotatably connected with a gear two (29), the inner middle side of the fixing seat (2) being fixedly connected with a toothed plate (10), and the inner wall of the fixing seat (2) being fixedly installed with a stopper (30).
3. The magnetic nanoparticle solid phase extraction column for pesticide residue detection according to claim 2, characterized in that: The gear one (9) and the gear two (29) are meshingly connected with the toothed plate (10).
4. The magnetic nanoparticle solid phase extraction column for pesticide residue detection according to claim 2, characterized in that: The sliding block (6) is slidably connected inside the sliding groove (7).
5. The magnetic nanoparticle solid phase extraction column for pesticide residue detection according to claim 1, characterized in that: The lower part of the extraction column body (1) is provided with a discharge port (20).
6. The magnetic nanoparticle solid phase extraction column for pesticide residue detection according to claim 1, characterized in that: A sealing ring (21) is arranged outside the blocking ball (22).
7. The magnetic nanoparticle solid phase extraction column for pesticide residue detection according to claim 1, characterized in that: The blocking ball (22) is slidably connected inside the pressure relief box (27).
8. The magnetic nanoparticle solid phase extraction column for pesticide residue detection according to claim 2, characterized in that: The mounting plate (5) is slidably connected inside the fixing seat (2).