Stem cell crushing device

Abstract

The utility model relates to the technical field of cell disruption, and particularly discloses a stem cell disruption device which comprises a box body and a disruption mechanism, the disruption mechanism comprises a placing plate, two mounting plates, a bidirectional threaded rod, a first motor, an ultrasonic generator, an ultrasonic probe, two groups of clamping assemblies, a plurality of groups of supporting assemblies, an abutting assembly and a sealing assembly, each supporting assembly comprises a round rod, a spring and a limiting block, the round rod penetrates through the containing plate, one end of the round rod is fixedly connected with the box body, the other end of the round rod is fixedly connected with the limiting block, one end of the spring is fixedly connected with the box body, and the other end of the spring is fixedly connected with the containing plate. And the two clamping assemblies are in threaded fit with the two-way threaded rod correspondingly, the abutting assembly is arranged above the containing plate, and through the arrangement, the container can be fixed, so that the situation that the container shakes and falls off during crushing is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of cell disruption, in particular to a stem cell disruption device. Background Art

[0002] An ultrasonic cell disruptor is a device that converts electrical energy into sound energy through a transducer. This energy forms a series of dense small bubbles in a liquid medium, and these small bubbles rapidly burst, generating energy like small bombs, thereby playing a role in disrupting cells and other substances. However, in existing ultrasonic disruptors, the position of the ultrasonic probe and the container for holding the cell solution are relatively fixed. After ultrasonic treatment, the liquid mixing rate in the container is relatively low, resulting in an unsatisfactory disruption effect.

[0003] To solve the above technical problems, the prior art patent (CN218478765U) discloses an ultrasonic disruptor for stem cell extraction, including components such as an equipment housing, a door body, an ultrasonic generating device, a driving component, an ultrasonic probe, and a placement table. The door body is arranged on the front side of the equipment housing in an openable and closable manner. A sealing frame corresponding to the shape of the door body is also provided on the equipment housing. The ultrasonic generating device is fixedly installed at the top of the equipment housing, and an ultrasonic probe is connected to the bottom of the ultrasonic generating device. The driving component is fixedly arranged in the middle of the bottom end of the inner cavity of the equipment housing, and the placement table is fixedly arranged on the top of the driving component. An auxiliary wire is also provided on the placement table. This ultrasonic disruptor for stem cell extraction achieves the effect of the ultrasonic probe stirring up and down in the container solution, significantly improving both the treatment effect and speed.

[0004] However, in the above prior art, when the placement table moves up and down, it is easy to cause the cell container placed above it to shake and fall, resulting in the scrapping of cells and the container. Summary of the Invention

[0005] The purpose of the utility model is to provide a stem cell disruption device, aiming to solve the technical problem in the prior art that when the placement table moves up and down, it is easy to cause the cell container placed above it to shake and fall, resulting in the scrapping of cells and the container.

[0006] To achieve the above object, a stem cell crushing device adopted by the present utility model includes a box body and a crushing mechanism. The crushing mechanism includes a placement plate, two mounting plates, a bidirectional threaded rod, a first motor, an ultrasonic generator, an ultrasonic probe, two groups of clamping components, multiple groups of support components, a resisting component, and a sealing component. Each group of the support components includes a round rod, a spring, and a limiting block. The ultrasonic generator is arranged on the upper surface of the box body. The ultrasonic probe is fixedly connected to the ultrasonic generator and inserted into the interior of the box body. The placement plate is arranged inside the box body. Multiple groups of the support components are respectively arranged at each vertex angle of the placement plate. The round rod penetrates through the placement plate and is slidably connected to the placement plate. One end of the round rod is fixedly connected to the box body, and the other end of the round rod is fixedly connected to the limiting block. The spring is sleeved outside the round rod. One end of the spring is fixedly connected to the box body, and the other end of the spring is fixedly connected to the placement plate. The two mounting plates are symmetrically arranged on the lower surface of the placement plate. The two ends of the bidirectional threaded rod are respectively rotatably connected to the two mounting plates. The output end of the first motor is fixedly connected to the bidirectional threaded rod. The two groups of clamping components are respectively in threaded cooperation with the bidirectional threaded rod. The resisting component is arranged above the placement plate, and the sealing component is arranged at one end of the box body.

[0007] Among them, each group of the clamping components includes a moving rod and a clamping plate. The moving rod penetrates through the placement plate and is slidably connected to the placement plate. One end of the moving rod is in threaded cooperation with the bidirectional threaded rod, and the other end of the moving rod is fixedly connected to the clamping plate.

[0008] Among them, the resisting component includes a second motor and a cam. The second motor is arranged outside the box body. The output end of the second motor penetrates through the box body and is fixedly connected to the cam.

[0009] Among them, the sealing component includes a box door, a handle, and an observation member. One end of the box door is hinged to the box body. The handle is fixedly connected to the box door and is located outside the box door. The observation member is arranged on one side of the box door.

[0010] Among them, the observation member includes a mounting frame, a transparent plate, and multiple bolts. The mounting frame penetrates through the box door. The multiple bolts respectively penetrate through the mounting frame and are respectively in threaded connection with the box door. The transparent plate is fixedly connected to the interior of the mounting frame.

[0011] Among them, the stem cell crushing device further includes two fixing plates, a grip rod, and an anti-slip sleeve. One end of each of the two fixing plates is respectively fixedly connected to the placement plate, and the other end of each of the two fixing plates is respectively fixedly connected to the grip rod. The anti-slip sleeve is sleeved outside the grip rod.

[0012] A stem cell crushing device of the present utility model, one end of the round rod is fixedly connected to the box body, the other end of the round rod is fixedly connected to the limit block, one end of the spring is fixedly connected to the box body, and the other end of the spring is fixedly connected to the placement plate. The output end of the first motor is fixedly connected to the bidirectional threaded rod, and the two clamping components are respectively in threaded cooperation with the bidirectional threaded rod. When specifically in use, press down the placement plate so that the placement plate slides on the round rod and presses against the first spring to contract. Then, place the container filled with the stem cell solution on the placement plate, and then release the placement plate. The spring rebounds, causing the placement plate to drive the container to move upward, so that the ultrasonic probe is inserted into the container. Then, start the first motor, and the first motor drives the bidirectional threaded rod to rotate, causing the two clamping components to move relatively, thereby clamping and fixing the container. Then, start the ultrasonic generator, so that the ultrasonic probe crushes the stem cells in the container. At the same time, under the action of the abutting component, the placement plate reciprocates up and down, so that the position of the ultrasonic probe in the container changes, thereby improving the crushing effect. This method can effectively solve the problem in the prior art that when the placement table moves up and down, it is easy to cause the cell container placed above it to shake and fall, resulting in the scrapping of the cells and the container. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] In order to more clearly illustrate the technical solutions in the embodiments of the present utility model or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the following drawings are only some embodiments of the present utility model. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0014] Figure 1 It is a schematic structural diagram of the first embodiment of the present utility model.

[0015] Figure 2 It is a partial structural diagram of the first embodiment of the present utility model.

[0016] Figure 3 It is a partial local structural diagram of the first embodiment of the present utility model.

[0017] Figure 4 It is a partial structural diagram of the second embodiment of the present utility model.

[0018] 101 - Box body, 102 - Placing plate, 103 - Mounting plate, 104 - Bidirectional threaded rod, 105 - First motor, 106 - Ultrasonic generator, 107 - Ultrasonic probe, 108 - Round rod, 109 - Spring, 110 - Limit block, 111 - Moving rod, 112 - Clamping plate, 113 - Second motor, 114 - Cam, 115 - Box door, 116 - Handle, 117 - Mounting frame, 118 - Transparent plate, 119 - Bolt, 201 - Fixed plate, 202 - Holding rod, 203 - Anti-slip sleeve. Detailed implementation mode

[0019] The embodiments of the present invention will be described in detail below. The examples of the embodiments are shown in the drawings. The embodiments described below with reference to the drawings are exemplary and are intended to explain the present invention, and should not be construed as a limitation to the present invention.

[0020] The first embodiment of the present application is as follows:

[0021] Please refer to Figures 1 to 3 , where Figure 1 is the structural schematic diagram of the first embodiment of the present invention, Figure 2 is the partial structural schematic diagram of the first embodiment of the present invention, Figure 3 is the local structural schematic diagram of the first embodiment of the present invention.

[0022] The present invention provides a stem cell crushing device, including a box body 101 and a crushing mechanism. The crushing mechanism includes a placing plate 102, two mounting plates 103, a bidirectional threaded rod 104, a first motor 105, an ultrasonic generator 106, an ultrasonic probe 107, two groups of clamping components, multiple groups of support components, a resisting component and a sealing component. Each group of the support components includes a round rod 108, a spring 109 and a limit block 110. Each group of the clamping components includes a moving rod 111 and a clamping plate 112. The resisting component includes a second motor 113 and a cam 114. The sealing component includes a box door 115, a handle 116 and an observation member. The observation member includes a mounting frame 117, a transparent plate 118 and multiple bolts 119. The foregoing solution solves the problem in the prior art that when the placing table moves up and down, it is easy to cause the cell container placed above it to shake and fall, resulting in the scrapping of the cells and the container.

[0023] For this specific embodiment, the ultrasonic generator 106 is disposed on the upper surface of the box body 101. The ultrasonic probe 107 is fixedly connected to the ultrasonic generator 106 and inserted into the interior of the box body 101. The placement plate 102 is disposed inside the box body 101. Multiple groups of the support components are respectively arranged at the respective top corners of the placement plate 102. The round rod 108 penetrates through the placement plate 102 and is slidably connected to the placement plate 102. One end of the round rod 108 is fixedly connected to the box body 101, and the other end of the round rod 108 is fixedly connected to the limiting block 110. The spring 109 is sleeved outside the round rod 108. One end of the spring 109 is fixedly connected to the box body 101, and the other end of the spring 109 is fixedly connected to the placement plate 102. Two mounting plates 103 are symmetrically arranged on the lower surface of the placement plate 102. Both ends of the bidirectional threaded rod 104 are rotatably connected to the two mounting plates 103 respectively. The output end of the first motor 105 is fixedly connected to the bidirectional threaded rod 104. Two groups of clamping components are respectively in threaded cooperation with the bidirectional threaded rod 104. The abutting component is disposed above the placement plate 102, and the sealing component is disposed at one end of the box body 101. When specifically in use, press down the placement plate 102, so that the placement plate 102 slides on the round rod 108 and abuts against the first spring 109 to contract. Then place the container filled with the stem cell solution on the placement plate 102, and then release the placement plate 102. The spring 109 rebounds, causing the placement plate 102 to drive the container to move upward, so that the ultrasonic probe 107 is inserted into the container. Then start the first motor 105. The first motor 105 drives the bidirectional threaded rod 104 to rotate, causing the two groups of clamping components to move relatively, thereby clamping and fixing the container. Then start the ultrasonic generator 106, so that the ultrasonic probe 107 breaks the stem cells in the container. At the same time, under the action of the abutting component, the placement plate 102 moves up and down reciprocally, so that the position of the ultrasonic probe 107 in the container changes, thereby improving the crushing effect.

[0024] Among them, the moving rod 111 penetrates through the placement plate 102 and is slidably connected to the placement plate 102. One end of the moving rod 111 is in threaded cooperation with the bidirectional threaded rod 104, and the other end of the moving rod 111 is fixedly connected to the clamping plate 112. When specifically in use, the bidirectional threaded rod 104 rotates, causing the moving rod 111 to move. The moving rod 111 drives the clamping plate 112 to move, thereby clamping and fixing the container.

[0025] Secondly, the second motor 113 is arranged outside the box body 101. The output end of the second motor 113 penetrates through the box body 101 and is fixedly connected to the cam 114. During actual use, when the second motor 113 is started, the output end of the second motor 113 drives the cam 114 to rotate, and the cam 114 abuts against the placement plate 102. With the cooperation of the spring 109, the placement plate 102 moves up and down repeatedly.

[0026] Meanwhile, one end of the box door 115 is hinged to the box body 101. The handle 116 is fixedly connected to the box door 115 and is located outside the box door 115. The viewing member is arranged on one side of the box door 115. During actual use, the box body 101 is sealed by the box door 115, and the box door 115 can be conveniently opened and closed through the handle 116.

[0027] In addition, the installation frame 117 penetrates through the box door 115. A plurality of bolts 119 respectively penetrate through the installation frame 117 and are threadedly connected to the box door 115. The transparent plate 118 is fixedly connected to the inside of the installation frame 117. During actual use, the installation frame 117 is fixed to the box door 115 by the plurality of bolts 119, the transparent plate 118 is installed through the installation frame 117, and the inside of the box body 101 can be conveniently observed through the transparent plate 118.

[0028] When using a stem cell crushing device according to this embodiment, during specific use, press down the placement plate 102 so that the placement plate 102 slides on the round rod 108 and presses against the first spring 109 to contract. Then, place a container filled with stem cell solution on the placement plate 102, and then release the placement plate 102. The spring 109 rebounds, causing the placement plate 102 to drive the container to move upward, so that the ultrasonic probe 107 is inserted into the container. Then, start the first motor 105, and the first motor 105 drives the bidirectional threaded rod 104 to rotate, causing the two moving rods 111 to move relative to each other. The two moving rods 111 respectively drive the corresponding clamping plates 112 to move, thereby clamping and fixing the container. Then, start the ultrasonic generator 106 so that the ultrasonic probe 107 crushes the stem cells in the container. At the same time, the output end of the second motor 113 drives the cam 114 to rotate, and the cam 114 presses against the placement plate 102. With the cooperation of the spring 109, the placement plate 102 moves up and down repeatedly, so that the position of the ultrasonic probe 107 in the container changes, thereby improving the crushing effect. This method can effectively solve the problem in the prior art that when the placement table moves up and down, it is easy to cause the cell container placed above it to shake and fall, resulting in the scrapping of the cells and the container.

[0029] The second embodiment of this application is as follows:

[0030] On the basis of the first embodiment, please refer to Figure 4 , Figure 4 which is a partial structural schematic diagram of the second embodiment of the present utility model.

[0031] The present utility model provides a stem cell crushing device, which further includes two fixing plates 201, a grip rod 202, and an anti-slip sleeve 203.

[0032] For this specific embodiment, one end of each of the two fixing plates 201 is fixedly connected to the placement plate 102, and the other end of each of the two fixing plates 201 is fixedly connected to the grip rod 202. The anti-slip sleeve 203 is sleeved on the outer side of the grip rod 202. During specific use, the two fixing plates 201 are used to fix the grip rod 202. The anti-slip sleeve 203 can enhance the friction of the grip rod 202, and the grip rod 202 facilitates pressing the placement plate 102.

[0033] When using a stem cell crushing device according to this embodiment, during specific use, hold the grip rod 202 and press down. The grip rod 202 drives the placement plate 102 to move downward through the two fixing plates 201, so as to facilitate pressing the placement plate 102.

[0034] The above-disclosed is only a preferred embodiment of the present utility model. Of course, it cannot be used to limit the scope of rights of the present utility model. Those of ordinary skill in the art can understand all or part of the processes of implementing the above embodiments, and the equivalent changes made according to the claims of the present utility model still fall within the scope covered by the utility model.

Claims

1. A stem cell fragmentation device, comprising a housing, characterized in that: It also includes a crushing mechanism, which includes a placement plate, two mounting plates, a bidirectional threaded rod, a first motor, an ultrasonic generator, an ultrasonic probe, two groups of clamping assemblies, multiple groups of support assemblies, abutment assemblies and a sealing assembly. The ultrasonic generator is arranged on the upper surface of the box body, the ultrasonic probe is fixedly connected to the ultrasonic generator and inserted into the interior of the box body, the placement plate is arranged inside the box body, multiple groups of support assemblies are respectively arranged at each vertex of the placement plate, each group of support assemblies includes a round rod, a spring and a limit block, the round rod passes through the placement plate and is slidably connected to the placement plate, and the round rod is connected to the placement plate. One end of the rod is fixedly connected to the box body, the other end of the round rod is fixedly connected to the limit block, the spring is sleeved on the outside of the round rod, one end of the spring is fixedly connected to the box body, the other end of the spring is fixedly connected to the placement plate, the two mounting plates are symmetrically arranged on the lower surface of the placement plate, the two ends of the bidirectional threaded rod are respectively rotatably connected to the two mounting plates, the output end of the first motor is fixedly connected to the bidirectional threaded rod, the two groups of the clamping assemblies are respectively threadedly matched with the bidirectional threaded rod, the supporting assembly is arranged above the placement plate, and the sealing assembly is arranged at one end of the box body.

2. The stem cell disruption device according to claim 1, characterized in that: Each group of the clamping assemblies includes a moving rod and a clamping plate. The moving rod passes through the placement plate and is slidably connected to the placement plate. One end of the moving rod is threadedly engaged with the bidirectional threaded rod, and the other end of the moving rod is fixedly connected to the clamping plate.

3. The stem cell disruption device according to claim 2, characterized in that: The abutting assembly includes a second motor and a cam. The second motor is arranged outside the box body. The output end of the second motor passes through the box body and is fixedly connected to the cam.

4. The stem cell disruption device according to claim 3, characterized in that: The sealing assembly includes a box door, a handle and an observation piece. One end of the box door is hinged to the box body. The handle is fixedly connected to the box door and is located on the outside of the box door. The observation piece is arranged on one side of the box door.

5. The stem cell disruption device according to claim 4, characterized in that: The observation piece includes a mounting frame, a transparent plate and a plurality of bolts. The mounting frame passes through the box door. The plurality of bolts pass through the mounting frame respectively and are respectively threadedly connected to the box door. The transparent plate is fixedly connected to the inside of the mounting frame.

6. The stem cell disruption device according to claim 5, characterized in that: The stem cell fragmentation device also includes two fixing plates, a gripping rod and an anti-slip sleeve, one end of the two fixing plates are respectively fixedly connected to the placement plates, the other ends of the two fixing plates are respectively fixedly connected to the gripping rods, and the anti-slip sleeve is sleeved on the outside of the gripping rods.

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