Deproteinization component and deproteinization device
By designing a deprotein component including a screen barrel, a support frame, a baffle and a rotating member, the problem of accumulation and destruction of heterogeneous bone particles during the deproteination process is solved, and more efficient deprotein effect and higher product quality are achieved.
Patent Information
- Application Number
- CN202421731685.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-22
- Publication Date
- 2025-06-17
- Estimated Expiration
- 2034-07-22
AI Technical Summary
In the existing deprotein technology, heterogeneous bone particles are prone to accumulation and destruction during the reaction, resulting in material damage and product waste.
A deprotein component is designed, including a screen barrel, a support frame, a baffle and a rotary member. By separating the bone particles and performing deprotein treatment without stirring, ensuring that the bone particles are in full contact with chemical reagents and avoiding material damage.
It effectively reduces the possibility of bone particles accumulation, improves the deprotein effect, reduces material damage and product waste, and improves product quality and volume.
Smart Images
Figure CN222983892U_ABST
Abstract
Description
Technical Field
[0001] This application belongs to the field of deproteinization equipment, and more particularly relates to a deproteinization component and a deproteinization device. Background Art
[0002] Xenogeneic bone, especially bovine bone, has become the main raw material for product development due to its similar composition and structure to human bone, and its good biocompatibility and safety. However, xenogeneic bone has the risk of immune rejection, so the bone particles with antigens removed have become the research focus.
[0003] Deproteinization is a key step in removing antigens, aiming to effectively remove cellular components such as antigens and proteins in xenogeneic bone while maintaining the three-dimensional reticular microstructure of the original bone tissue, so as to facilitate the growth of host bone cells along the support frame into the pores and complete the repair of bone defects. At present, the deproteinization method of xenogeneic bone is to place chemical reagents and xenogeneic bone particles in a reaction kettle, and through the stirring of a stirring paddle, promote the full contact between the chemical reagents and xenogeneic bone particles, and achieve the deproteinization effect through multiple cycle reactions.
[0004] However, in the above deproteinization process, a large number of bone particles accumulate at the bottom of the reaction kettle, and the stirring of the stirring paddle easily damages the bone particles and causes material damage. Utility Model Content
[0005] In view of the above-mentioned defects and problems of the prior art, this application has developed a deproteinization component and a deproteinization device.
[0006] In the first aspect of this application, a deproteinization component is provided, including a sieve barrel, a support frame, a baffle and a rotating member. The support frame is connected to the outer wall of the sieve barrel, the baffle is rotatably connected inside the sieve barrel, the baffle rotates around its horizontal axis, the outer peripheral wall of the baffle fits with the inner peripheral wall of the sieve barrel, and the rotating member is connected to the baffle and is used to drive the baffle to rotate.
[0007] Optionally, the baffle is provided with through holes penetrating the wall thickness.
[0008] Optionally, the rotating member includes a damping sleeve and a first handle. The damping sleeve is connected to the rotating connection between the baffle and the support frame, and the first handle is used to drive the baffle to rotate.
[0009] Optionally, the rotating member includes a torsion spring and a second handle. The torsion spring is connected to the rotating connection between the baffle and the support frame, and the second handle is used to drive the baffle to rotate.
[0010] Optionally, the support frame includes a vertical rod and a horizontal rod, and the vertical rod and the horizontal rod intersect with each other and are both connected to the outer wall of the sieve barrel.
[0011] Optionally, the width of the cross bar and the vertical bar is 2 mm - 6 mm.
[0012] Optionally, the aperture diameter of the through hole is 0.5 mm - 1.5 mm.
[0013] Optionally, it further includes a handle, the handle is connected to the outer wall of the sieve barrel, and the materials of the handle, the sieve barrel, the support frame, and the baffle are all 304 stainless steel.
[0014] Optionally, the sieve barrel is a hollow cylinder with an open top wall.
[0015] On the other hand, this application also provides a deproteinization device, which includes a reaction kettle and the deproteinization component described in any one of the above first aspects, and the deproteinization component is placed in the reaction kettle.
[0016] In summary, this application has the following beneficial effects:
[0017] After dividing the bone particles to be deproteinized into two parts and placing them at the bottom of the sieve barrel and above the baffle respectively, during the deproteinization process, even without stirring, the possibility of bone particle accumulation can be reduced, enabling the bone particles to come into full contact with the chemical reagent, thus promoting the deproteinization effect of the bone particles. Moreover, this process will not damage the bone particles due to stirring, nor will it cause the deproteinized bone particles to be too small in particle size to be effectively collected later, resulting in waste, thereby improving the product quality and yield. Description of the Drawings
[0018] In order to more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art.
[0019] Figure 1 It is a cross-sectional view of the deproteinization component in some embodiments of this application;
[0020] Figure 2 It is a cross-sectional view of the deproteinization component in another embodiment of this application;
[0021] Figure 3 It is a cross-sectional view of the deproteinization component in another embodiment of this application;
[0022] Figure 4 It is a schematic diagram of the positional relationship between the deproteinization component and the reaction kettle in the deproteinization device of this application.
[0023] Reference numerals: 100, deproteinization component; 110, sieve barrel; 120, support frame; 121, vertical rod; 122, cross rod; 130, baffle; 131, through hole; 140, rotating part; 141, damping sleeve; 142, first handle; 143, torsion spring; 144, second handle; 150, handle; 160, rotating shaft; 200, reaction kettle. Detailed implementation manners
[0024] The following further elaborates on the present application in combination with embodiments. It should be specifically noted that: for those not specifying specific conditions in the following embodiments, they are carried out according to conventional conditions or conditions recommended by the manufacturer.
[0025] To make the above objects, features, and advantages of the present application more obvious and understandable, the following provides a detailed description of the specific implementation manners of the present application. Many specific details are set forth in the following description to facilitate a full understanding of the present application. However, the present application can be implemented in many other ways different from those described herein. Those skilled in the art can make similar improvements without departing from the connotation of the present application. Therefore, the present application is not limited by the specific embodiments disclosed below.
[0026] In the present application, "the first aspect", "the second aspect", "the third aspect", etc. are only for descriptive purposes and should not be construed as indicating or implying relative importance or quantity, nor should they be construed as implicitly indicating the importance or quantity of the indicated technical features. Moreover, "the first", "the second", "the third", etc. only serve the purpose of non-exhaustive enumerative description and should be understood not to constitute a closed limitation on quantity.
[0027] In the present application, regarding numerical ranges, unless otherwise specified, the above numerical ranges are considered continuous and include the minimum and maximum values of the range, as well as each value between such minimum and maximum values. Further, when the range refers to integers, it includes each integer between the minimum and maximum values of the range. In addition, when multiple ranges are provided to describe features or characteristics, these ranges can be combined. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all sub-ranges subsumed therein.
[0028] In the present application, for the technical features described in an open-ended manner, it includes a closed technical solution composed of the listed features, as well as an open technical solution including the listed features.
[0029] Such as Figure 1As shown in the figure, a deproteinization component 100 includes a sieve barrel 110, a support frame 120, a baffle 130, and a rotating member 140. The support frame 120 is connected to the outer wall of the sieve barrel 110 to support the sieve barrel 110. The baffle 130 is rotatably connected inside the sieve barrel 110. The baffle 130 rotates around its horizontal axis, and the outer peripheral wall of the baffle 130 fits against the inner peripheral wall of the sieve barrel 110. The rotating member 140 is fixedly connected to the baffle 130 and is used to drive the baffle 130 to rotate.
[0030] During use, the baffle 130 is rotated by the rotating member 140 to leave a gap between the outer peripheral wall of the baffle 130 and the inner wall of the sieve barrel 110. A part of the bone particles to be deproteinized is poured into the bottom of the sieve barrel 110, and then the baffle 130 is reset by the rotating member 140, and the remaining bone particles are poured onto the baffle 130. Then, the entire sieve barrel 110 is placed in a reaction container, and a liquid chemical reagent is added to the reaction container. At this time, the chemical reagent will enter the sieve barrel 110 through the sieve holes on the sieve barrel 110 and submerge the bone particles above the baffle 130 and at the bottom of the sieve barrel 110 at the same time. When the deproteinization reaction is completed, the sieve barrel 110 is taken out of the reaction container, and then the baffle 130 is rotated by the rotating member 140 to leave a gap between the outer wall of the baffle 130 and the inner wall of the sieve barrel 110, and all the deproteinized bone particles in the sieve barrel 110 are poured out to complete the operation of deproteinizing the bone particles.
[0031] It can be understood that the sieve barrel 110 can be made of a metal sieve mesh, the sieve mesh is woven precisely and the mesh holes are regular. The aperture of the sieve holes on the sieve barrel 110 is smaller than the particle size of the bone particles. The height of the baffle 130 is lower than the liquid level of the chemical reagent in the reaction container, and the height of the sieve barrel 110 is adapted to the height of the reaction container. As an example, the aperture of the sieve holes on the sieve barrel 110 can be set to 0.5 mm - 1.5 mm, and the height of the sieve barrel 110 can be 1 cm higher than the liquid level height of the chemical reagent.
[0032] During the above deproteinization process, after dividing the bone particles to be deproteinized into two parts, it helps to make the bone particles fully contact with the chemical reagent without stirring, which helps to ensure the deproteinization effect of the bone particles. And during the whole deproteinization process, the bone particles will not be damaged due to stirring, reducing the damage of the bone particles, nor will the deproteinized bone particles be too small in particle size to be effectively collected in the later stage, thus avoiding waste.
[0033] In some possible implementation manners, in order to further promote the full contact between the bone particles and the chemical reagent, as Figure 2 shown in the figure, the baffle 130 is provided with through holes 131 penetrating the wall thickness, and the aperture of the through holes 131 is smaller than the particle size of the bone particles. As an example, the aperture of the through holes 131 can be set to 0.5 mm - 1.5 mm.
[0034] It can be understood that a rotating shaft 160 is integrally connected to a set of symmetric side walls of the baffle 130. After passing through the side wall of the sieve barrel 110, the rotating shaft 160 is rotatably connected to the support frame 120. In addition, the baffle 130 and the rotating member 140 can be arranged in parallel in multiple numbers according to actual needs.
[0035] In some possible implementation manners, such as Figure 2 As shown, the rotating member 140 includes a damping sleeve 141 and a first handle 142. The damping sleeve is sleeved on the rotating shaft 160 and is located at the connection between the rotating shaft 160 and the support frame 120. The first handle 142 is integrally connected to the rotating shaft 160. At this time, rotating the first handle 142 can rotate the baffle 130, and under the action of non-human force, the baffle 130 is not easy to rotate under the action of the damping sleeve 141.
[0036] In other possible implementation manners, such as Figure 3 As shown, the rotating member 140 includes a torsion spring 143 and a second handle 144. The torsion spring 143 is sleeved on the rotating shaft 160 and is located at the connection between the rotating shaft 160 and the support frame 120. The second handle 144 is integrally connected to the rotating shaft 160. At this time, under the action of non-human force, the baffle 130 is not easy to rotate under the action of the torsion spring 143. When the second handle 144 is rotated, the baffle 130 can be rotated; after the rotation is completed and the hand is released, the baffle 130 will reset under the elastic force of the torsion spring 143, which is convenient for operation and also helps to reduce the workload of the operator. It can be understood that in this embodiment, the initial position of the baffle 130 can be horizontal or at an angle with the horizontal (such as perpendicular to the horizontal plane), and no specific limitation is made here.
[0037] The sieve barrel 110 can be set to any shape adapted to the reaction vessel. For example, the sieve barrel 110 can be a hollow prism with an open top wall, or the sieve barrel 110 can also be an inverted hollow frustum with an open top wall. In this embodiment, the sieve barrel 110 is a hollow cylinder with an open top wall. At this time, the diameter of the baffle 130 is the same as the diameter of the bottom wall of the sieve barrel 110. Therefore, the bone particles can be evenly divided into two parts, and the sampling process is relatively convenient. Moreover, the bottom wall of the cylindrical sieve barrel 110 has a relatively large loading capacity, which can reduce the situation of bone particles piling up with each other and promote the deproteinization of bone particles.
[0038] Such as Figure 3 As shown, the support frame 120 can include a vertical rod 121 and a horizontal rod 122. The vertical rod 121 and the horizontal rod 122 intersect with each other and are welded to the outer wall of the sieve barrel 110. The horizontal rod 122 and the vertical rod 121 mainly play a role in supporting the sieve barrel 110. The widths of the horizontal rod 122 and the vertical rod 121 can be 2 mm - 6 mm. As an example, the widths of the horizontal rod 122 and the vertical rod 121 are 5 mm. It can be understood that the bottom wall of the sieve barrel 110 can also be fixedly connected with a bottom bracket for supporting the bottom of the sieve barrel 110.
[0039] To facilitate the grasping and transferring of the sieve barrel 110 and avoid direct human contact with chemical reagents, a handle 150 is also connected to the top of the sieve barrel 110 by screws. The handle 150 can also be connected to the support frame 120. In addition, the handle 150, the sieve barrel 110, the support frame 120, and the baffle 130 can be made of 304 stainless steel, which has stable performance and is not likely to introduce impurities.
[0040] As Figure 4 shown, a deproteinization device of the present application includes a reaction kettle 200 and the above-mentioned deproteinization component 100. The deproteinization component 100 is placed in the reaction kettle 200. Combining Figure 3 and Figure 4 , the usage method of the deproteinization device is as follows:
[0041] (1) Divide the bone particles into two parts and place them at the bottom of the sieve barrel 110 and on the baffle 130 in the deproteinization component 100 respectively;
[0042] (2) Place the deproteinization component 100 into the reaction kettle 200;
[0043] (3) Put chemical reagents into the reaction kettle 200. The chemical reagents submerge the bone particles at the bottom of the sieve barrel 110 and on the baffle 130 to achieve the leaching of the bone particles. Multiple leaching operations can be carried out. After each leaching operation ends, open the bottom valve of the reaction kettle 200 to discharge the reaction solution, and directly flush the bone particles by adding water from the upper end of the reaction kettle 200;
[0044] (4) When the entire leaching process ends, hold the handle 150 by hand, remove the deproteinization component 100, and flip the baffle 130 through the rotating part 140 to merge the materials on the baffle 130 into the bottom of the sieve barrel 110, so as to remove all the bone particles in the sieve barrel 110 and complete the deproteinization process.
[0045] It should be understood that the present application disclosed is not limited to the specific methods, schemes, and substances described, as these can all vary. It should also be understood that the terms used herein are only for the purpose of describing specific embodiments and are not intended to limit the scope of the present application. The scope of the present application is only limited by the appended claims.
[0046] Those skilled in the art will also recognize, or be able to confirm using no more than routine experimentation, many equivalents of the specific embodiments of the present application described herein. These equivalents are also included in the appended claims.
Claims
1. A deproteinization component, characterized in that: It includes a sieve barrel, a support frame, a baffle and a rotating member, the support frame is connected to the outer wall of the sieve barrel, the baffle is rotatably connected to the inside of the sieve barrel, the baffle rotates around its horizontal axis, the outer circumferential wall of the baffle is in contact with the inner circumferential wall of the sieve barrel, and the rotating member is connected to the baffle and is used to drive the baffle to rotate.
2. A deproteinization component according to claim 1, characterized in that: The baffle is provided with a through hole penetrating the wall thickness.
3. A deproteinization component according to claim 1, characterized in that: The rotating member includes a damping sleeve and a first handle. The damping sleeve is connected to the rotation connection between the baffle and the support frame. The first handle is used to drive the baffle to rotate.
4. A deproteinization component according to claim 1, characterized in that: The rotating member includes a torsion spring and a second handle, wherein the torsion spring is connected to the rotation connection between the baffle and the support frame, and the second handle is used to drive the baffle to rotate.
5. A deproteinization component according to claim 1, characterized in that: The support frame includes a vertical rod and a horizontal rod, and the vertical rod and the horizontal rod are staggered with each other and are both connected to the outer wall of the screen barrel.
6. A deproteinization component according to claim 5, characterized in that: The width of the cross bar and the vertical bar is 2mm-6mm.
7. A deproteinization component according to claim 2, characterized in that: The through hole has a diameter of 0.5 mm to 1.5 mm.
8. A deproteinization component according to claim 1, characterized in that: It also includes a handle, which is connected to the outer wall of the sieve barrel. The handle, the sieve barrel, the support frame and the baffle are all made of 304 stainless steel.
9. A deproteinization component according to claim 1, characterized in that: The sieve barrel is a hollow cylinder with an open top wall.
10. A deproteinization device, characterized in that: The invention comprises a reaction kettle and the deproteinization component according to any one of claims 1 to 9, wherein the deproteinization component is placed in the reaction kettle.