Micro-fluidic chip

Abstract

The micro-fluidic chip comprises a disc and a diluent bag, the disc is provided with a containing cavity and a diluent cavity, and the rotating axis of the disc penetrates through the containing cavity; the accommodating cavity is provided with a diluent outlet communicated with the diluent cavity; the diluent bag is provided with a diluent opening, and the diluent bag is arranged in the containing cavity; and the disc is provided with an eccentric structure. Due to the fact that the disc is provided with the eccentric structure, the eccentric structure enables the central axis of the diluent bag to be closer to the diluent opening than the rotating axis, namely, the eccentric structure enables the diluent bag to be eccentrically arranged relative to the rotating axis, and the diluent opening of the diluent bag is far away from the rotating axis, when the disc rotates centrifugally, under the effect of centrifugal force, the diluent bag can be separated from the diluent opening. The diluent in the eccentric diluent bag centrifugally moves towards the diluent opening, so that the diluent in the diluent bag is completely released; in the process of rotation, acceleration, deceleration, left-right swing and the like of the disc, secondary release of the diluent cannot be generated, the dilution concentration of a sample can be guaranteed, and then the accuracy of a test result is guaranteed.

Description

Technical Field

[0001] The utility model relates to the technical field of in vitro detection, and particularly relates to a microfluidic chip. Background Art

[0002] Point-of-care testing (POCT), as a subdivision in the field of in vitro diagnosis, has been widely applied in the fields of clinical inspection, personal health management, disease prevention and monitoring, etc. due to its characteristics of miniaturized instruments, rapid detection, and simplified operation.

[0003] Biochemical point-of-care testing mainly adopts centrifugal microfluidic technology. Through specific centrifugal motion of a microfluidic chip inside a biochemical analyzer, the whole process of biochemical detection operations such as sample quantification, diluent quantification, sample mixing, sample injection, and sample detection is realized.

[0004] Among them, by installing a diluent package in the installation groove of a disc, the diluent in the diluent package is released into the diluent cavity through centrifugal force. However, currently, the diluent package does not release the diluent completely, which will cause the disc to release the diluent for the second time during the processes of rotation, acceleration, deceleration, left - right swing, etc., and further cause the sample to be diluted by the second diluent or the second diluent in the reagent reaction system, resulting in a lower test result. Content of the Utility Model

[0005] A microfluidic chip of the utility model is used to solve the problem that the incomplete release of the diluent from the diluent package leads to a lower measurement result.

[0006] In one embodiment, a microfluidic chip is provided, which includes a disc and a diluent package. The disc is provided with a receiving cavity and a diluent cavity. The receiving cavity is located in the middle of the disc, and the rotation axis of the disc passes through the receiving cavity;

[0007] The receiving cavity is provided with a diluent outlet communicating with the diluent cavity;

[0008] The diluent package has a diluent opening, and the diluent package is arranged in the receiving cavity;

[0009] The disc is provided with an eccentric structure, and the eccentric structure is such that the central axis of the diluent package is closer to the diluent opening than the rotation axis.

[0010] In one embodiment, the eccentric structure is arranged in the receiving cavity.

[0011] In one embodiment, the eccentric structure positions the diluent package in the receiving cavity.

[0012] In one embodiment, the eccentric structure includes one or more eccentric positioning members that squeeze the circumferential side surface of the diluent package to move the central axis of the diluent package closer to the diluent opening than the rotation axis and to position the diluent package within the accommodating cavity.

[0013] In one embodiment, a plurality of the eccentric positioning members are distributed around the diluent package, and the plurality of eccentric positioning members clamp and position the diluent package within the accommodating cavity.

[0014] In one embodiment, one or more of the eccentric positioning members are distributed on the circumferential side surface of the diluent package, and one or more of the eccentric positioning members and the side wall of the accommodating cavity clamp and position the diluent package within the accommodating cavity.

[0015] In one embodiment, among the plurality of eccentric positioning members, the farther away from the diluent opening, the thicker the thickness of the eccentric positioning member in the direction perpendicular to the rotation axis.

[0016] In one embodiment, the eccentric positioning member has an eccentric positioning surface for squeezing the circumferential side surface of the diluent package.

[0017] In one embodiment, the area of the eccentric positioning surface gradually increases along the direction in which the diluent package is inserted into the accommodating cavity.

[0018] In one embodiment, the accommodating cavity is a hollow cylindrical cavity, and the central axis of the hollow cylindrical cavity coincides with the rotation axis.

[0019] In one embodiment, the outer surface of the diluent package is cylindrical.

[0020] According to the microfluidic chip of the above embodiment, since the disc is provided with an eccentric structure, the eccentric structure makes the central axis of the diluent package closer to the diluent opening than the rotation axis, that is, the eccentric structure makes the diluent package eccentrically arranged relative to the rotation axis, and the diluent opening of the diluent package is far from the rotation axis. When the disc rotates centrifugally, under the action of centrifugal force, the diluent in the eccentric diluent package will move centrifugally towards the diluent opening, so that the diluent in the diluent package is completely released; during the rotation, acceleration, deceleration, left - right swing, etc. of the disc, there will be no secondary release of the diluent, which can ensure the dilution concentration of the sample and thus ensure the accuracy of the test results. Description of the Drawings

[0021] Figure 1 It is a schematic structural diagram of a microfluidic chip in one embodiment;

[0022] Figure 2 It is a schematic structural diagram of a microfluidic chip in one embodiment;

[0023] Figure 3 It is a schematic diagram of a partial structure of a microfluidic chip in an embodiment;

[0024] Figure 4 It is a schematic diagram of the structure of a cover body in an embodiment;

[0025] Figure 5 It is a schematic diagram of the structure of a microfluidic chip in an embodiment;

[0026] Figure 6 It is a schematic diagram of the structure of a microfluidic chip in an embodiment;

[0027] Figure 7 It is a schematic diagram of the structure of a microfluidic chip in an embodiment;

[0028] Figure 8 It is a schematic diagram of the structure of a microfluidic chip in an embodiment;

[0029] Among them, the reference numerals are as follows:

[0030] 100 - disc, 101 - disc body, 102 - cover plate, 103 - piercing part, 200 - diluent package, 201 - diluent opening;

[0031] 1 - accommodation cavity, 11 - diluent outlet, 12 - eccentric structure, 121 - eccentric positioning member, 122 - eccentric positioning surface, 2 - diluent cavity, 3 - diluent metering cavity, 4 - sample injection cavity, 5 - sample liquid metering cavity, 6 - mixing cavity, 7 - detection hole;

[0032] A - rotation axis, B - central axis. Detailed implementation manners

[0033] The present utility model will be further described in detail below in conjunction with the accompanying drawings through specific implementation manners. Similar elements in different implementation manners adopt related similar element numbers. In the following implementation manners, many detailed descriptions are for enabling a better understanding of the present application. However, those skilled in the art can easily recognize that some of the features can be omitted in different situations, or can be replaced by other elements, materials, and methods. In some cases, some operations related to the present application are not shown or described in the specification, which is to avoid the core part of the present application being submerged by excessive descriptions. For those skilled in the art, it is not necessary to describe these related operations in detail, and they can fully understand the related operations according to the descriptions in the specification and the general technical knowledge in the art.

[0034] In addition, the features, operations, or characteristics described in the specification can be combined in any suitable manner to form various embodiments. At the same time, the steps or actions in the method description can also be reordered or adjusted in a manner obvious to those skilled in the art. Therefore, the various sequences in the specification and drawings are only for clearly describing a certain embodiment and do not mean a necessary sequence, unless it is stated otherwise that a certain sequence must be followed.

[0035] The serial numbers assigned to the components herein, such as "first", "second", etc., are only used to distinguish the objects described and do not have any sequential or technical meaning. And as used in this application, "connection" and "coupling", unless otherwise specified, both include direct and indirect connection (coupling).

[0036] In one embodiment, a microfluidic chip is provided. This microfluidic chip is a disc for biochemical point-of-care testing. This microfluidic chip mainly uses centrifugal microfluidic technology to achieve detection. Through the specific centrifugal motion of the microfluidic chip inside a biochemical analyzer, the full process operations of biochemical detection such as sample quantification, diluent quantification, sample mixing, sample injection, and sample detection are realized.

[0037] In this embodiment, a receiving cavity is provided in the middle of the disc. The receiving cavity is used to install a diluent package, and the diluent package contains diluent. The disc is provided with an eccentric structure. The eccentric structure can make the diluent package eccentric relative to the rotation axis of the disc, and the diluent opening of the diluent package is far from the rotation axis. When the disc rotates centrifugally, under the action of centrifugal force, the diluent in the eccentric diluent package will move centrifugally towards the diluent opening direction, so that the diluent in the diluent package is completely released; during the rotation, acceleration, deceleration, left and right swing, etc. of the disc, no secondary release of the diluent will occur, which can ensure the dilution concentration of the sample and thus ensure the accuracy of the test results.

[0038] Furthermore, the eccentric structure is arranged in the receiving cavity. The eccentric structure also has the function of positioning and installation. The eccentric structure can position the diluent package in the receiving cavity of the disc, and the eccentric structure simultaneously serves the functions of eccentricity and positioning and installation.

[0039] Please refer to Figures 1 to 4 , the microfluidic chip of this embodiment mainly includes a disc 100 and a diluent package 200. The outer surface of the diluent package 200 can be cylindrical, and the diluent package 200 contains diluent. A diluent opening 201 is provided at the edge of the axial end face of the diluent package 200. The diluent opening 201 can be set as a structure that can be torn or pierced, for example, the diluent opening 201 of the diluent package 200 is a thin film structure such as aluminum foil.

[0040] The disc 100 has a disc-shaped structure. The disc-shaped disc 100 is conducive to realizing the full process operations of biochemical detection such as sample quantification, diluent quantification, sample mixing, sample injection, and sample detection during the centrifugal movement.

[0041] The disc 100 is provided with a receiving cavity 1 and a diluent cavity 2. The disc 100 is also provided with necessary structures for detection such as a diluent quantification cavity 3, a sample injection cavity 4, a sample liquid quantification cavity 5, a mixing cavity 6, a detection hole 7, and a capillary.

[0042] The disc 100 has a rotation axis A, and the rotation axis A of the disc 100 coincides with the center line of the disc 100, so that the disc 100 can maintain relative stability during centrifugal rotation.

[0043] The diluent package 200 is detachably installed in the receiving cavity 1. The receiving cavity 1 is arranged in the middle of the disc 100. The receiving cavity 1 can be a hollow cylindrical cavity. The rotation axis A of the disc 100 passes through the receiving cavity 1, and the receiving cavity 1 has a central axis B. Specifically, the receiving cavity 1 is located at the center of the disc 100, and the central axis B of the receiving cavity 1 coincides with the rotation axis A of the disc 100. Such a setting is conducive to the diluent quantification cavity 3, the sample injection cavity 4, the sample liquid quantification cavity 5, the mixing cavity 6, and the detection hole 7 being arranged around the receiving cavity 1 in a structure evenly distributed from the center to the periphery.

[0044] In other embodiments, the central axis B of the receiving cavity 1 can also deviate a certain distance from the rotation axis A of the disc 100.

[0045] In this embodiment, both the receiving cavity 1 and the diluent package 200 are located in a cylindrical structure, which can better adapt to the centrifugal rotation of the disc 100 and maintain the stability of the disc 100.

[0046] In other embodiments, the receiving cavity 1 and the diluent package 200 can also be other shaped structures. For example, the receiving cavity 1 is a hollow polygonal column cavity, and the outer surface of the diluent package 200 can also be polygonal columnar. The axial cross-sections of the hollow polygonal column cavity and the polygonal columnar are polygons. Setting the receiving cavity 1 and the diluent package 200 as other shaped structures can also realize the centrifugal rotation detection of the disc 100.

[0047] In this embodiment, the receiving cavity 1 is a relatively closed chamber. The receiving cavity 1 is only provided with a diluent outlet 11 communicating with the cavity inside the receiving cavity 1, so that the diluent flowing out of the diluent package 200 installed in the receiving cavity 1 can only flow out from the diluent outlet 11.

[0048] The diluent outlet 11 is located at a position of the receiving cavity 1 far from the rotation axis A. For example, the diluent outlet 11 is located on the circumferential side surface of the receiving cavity 1. The receiving cavity 1 is communicated with the diluent cavity 2 through the diluent outlet 11. The diluent cavity 2 is used to accommodate and transfer the diluent.

[0049] The diluent opening 201 of the diluent package 200 is aligned and connected with the diluent outlet 11 along the radial direction of the accommodating chamber 1, so that the diluent in the diluent package 200 can directly enter the diluent chamber 2 through the diluent outlet 11 after flowing out from the diluent opening 201, thereby preventing the diluent from being retained in the accommodating chamber 1.

[0050] In other embodiments, the diluent opening 201 of the diluent package 200 and the diluent outlet 11 are partially or completely staggered along the radial direction of the accommodating chamber 1. The diluent opening 201 of the diluent package 200 can also be connected to the diluent outlet 11 through the accommodating chamber 1. Under the action of the centrifugal force of the disc 100, the diluent can also enter the diluent chamber 2 through the diluent outlet 11.

[0051] In this embodiment, the diluent quantitative chamber 3 can be connected to the diluent chamber 2 through a capillary, so that the diluent in the diluent chamber 2 flows into the diluent quantitative chamber 3. The diluent quantitative chamber 3 is used to quantify the diluent, and the diluent quantitative chamber 3 can be connected to a chamber such as a diluent waste chamber for collecting the diluent. After the excessive diluent enters the diluent quantitative chamber 3, it will overflow to the diluent waste chamber, so as to ensure that the quantified diluent in the diluent quantitative chamber 3 is the amount required for the test, and ensure that the dilution ratio of the sample is accurate.

[0052] The sample injection chamber 4 is used for injecting sample liquid. The sample injection chamber 4 may be provided with an injection port, through which the sample liquid may be injected into the sample injection chamber 4 .

[0053] The sample liquid quantitative chamber 5 can be connected to the sample injection chamber 4 through a capillary tube, so that the sample liquid in the sample injection chamber 4 flows into the sample liquid quantitative chamber 5. The sample liquid quantitative chamber 5 is used to quantify the sample liquid. The sample liquid quantitative chamber 5 can be connected to a chamber for collecting the sample liquid, such as a sample liquid waste chamber. Excessive sample liquid will overflow into the sample liquid waste chamber after entering the sample liquid quantitative chamber 5, so as to ensure that the sample liquid quantified in the sample liquid quantitative chamber 5 is the amount required for the test, and ensure that the sample dilution ratio is accurate.

[0054] The mixing chamber 6 provides an environment for diluting and mixing the sample liquid and the diluent. The sample liquid and the diluent enter the mixing chamber 6 and mix to form a mixed liquid. The mixing chamber 6 is connected to the sample liquid quantitative chamber 5 through a first capillary. One end of the first capillary is connected to the sample liquid quantitative chamber 5, and the other end of the first capillary is connected to the mixing chamber 6. The first capillary is used to guide the sample liquid in the sample liquid quantitative chamber 5 to the mixing chamber 6.

[0055] The mixing chamber 6 is connected to the diluent metering chamber 3 through a second capillary tube. One end of the second capillary tube is connected to the diluent metering chamber 3, and the other end of the second capillary tube is connected to the mixing chamber 6. The second capillary tube is used to guide the diluent in the diluent metering chamber 3 into the mixing chamber 6.

[0056] The mixing chamber 6 is connected to a plurality of detection holes 7 through a third capillary tube and a liquid distribution chamber. The liquid distribution chamber can be provided with one inlet and a plurality of outlets. The inlet of the liquid distribution chamber is connected to the mixing chamber 6 through the third capillary tube, and the plurality of outlets of the liquid distribution chamber are in one-to-one correspondence with the plurality of detection holes 7. The liquid distribution chamber can distribute the mixed liquid discharged from the mixing chamber 6 into the plurality of detection holes 7, and the plurality of detection holes 7 can realize simultaneous detection of multiple items.

[0057] In this embodiment, the disc 100 is further provided with an eccentric structure 12. The eccentric structure 12 is arranged in the accommodating cavity 1. The eccentric structure 12 squeezes and contacts the diluent package 200, so that the central axis B of the diluent package 200 is closer to the diluent opening 201 than the rotation axis A of the disc 100, that is, the eccentric structure 12 makes the diluent package 200 eccentrically arranged relative to the accommodating cavity 1, and the diluent opening 201 is located at a position relatively far from the rotation axis A of the disc 100. When the disc 100 rotates, the diluent in the eccentric diluent package 200 will obtain a greater centrifugal driving force. The diluent will not rotate in the diluent package, but move towards the direction of the diluent opening 201. Furthermore, the diluent can completely flow out of the diluent package 200, and the residue of the diluent in the diluent package 200 can be avoided.

[0058] The eccentric structure 12 in this embodiment also has the function of positioning and installing the diluent package 200. The eccentric structure 12 positions and fixes the diluent package 200 in the accommodating cavity 1. The eccentric structure 12 simultaneously plays the roles of eccentricity and positioning and fixing, and thus the structure of the disc 100 can be simplified without separately arranging a positioning and installing structure.

[0059] The eccentric structure 12 includes a plurality of eccentric positioning members 121. The plurality of eccentric positioning members 121 are arranged around the diluent package 200. The plurality of eccentric positioning members 121 are located on a circumference of the accommodating cavity 1. The center line of this circumference does not coincide with the rotation axis A of the disc 100, and the center line of this circumference is closer to the diluent opening 201 than the rotation axis A of the disc 100. The diluent package 200 is positioned within this circumference. The plurality of eccentric positioning members 121 clamp and squeeze the circumferential side surface of the diluent package 200. The diluent package 200 can be axially installed parallel to the accommodating cavity 1 within the circumference enclosed by the plurality of eccentric positioning members 121, and the central axis B of the diluent package 200 coincides with the center line of the circumference enclosed by the plurality of eccentric positioning members 121.

[0060] The number of the eccentric positioning members 121 can be set as needed, such as Figure 3As shown, six eccentric positioning members 121 may be provided.

[0061] The eccentric positioning member 121 may be a rib protruding from the side wall of the accommodating cavity 1 or the like, and the eccentric positioning member 121 may also be a rib structure extending from the top wall or the bottom wall of the accommodating cavity 1 into the accommodating cavity 1.

[0062] The eccentric positioning member 121 has an eccentric positioning surface 122, and the eccentric positioning surface 122 is the side surface of a boss. The eccentric positioning member 121 squeezes the circumferential side surface of the diluent package 200 through the eccentric positioning surface 122. The eccentric positioning member 121 squeezes and positions the diluent package 200 in a surface contact manner, which can increase the contact area between the eccentric positioning member 121 and the diluent package 200, and further improve the stability of the eccentric positioning member 121 in fixing the diluent package 200.

[0063] In other embodiments, the eccentric positioning member 121 may also be diamond-shaped or hemispherical. The corresponding eccentric positioning member 121 has a positioning line or a positioning point. The contact between the eccentric positioning member 121 and the diluent package 200 is line contact or point contact. Using line contact or point contact can also squeeze the diluent package 200 to form eccentricity and play a fixing role.

[0064] In this embodiment, multiple eccentric positioning members 121 may be evenly distributed around the diluent package 200, and multiple eccentric positioning members 121 jointly complete the eccentric setting and positioning installation of the diluent package 200.

[0065] In this embodiment, multiple eccentric positioning members 121 are arranged on the side wall of the accommodating cavity 1. In order to enable multiple eccentric positioning members 121 to enclose an eccentric circle, multiple eccentric positioning members 121 have different thicknesses. Among them, the farther the eccentric positioning member 121 is from the diluent opening 201, the greater its thickness. The thickness direction of the eccentric positioning member 121 is perpendicular to the rotation axis A, that is, the thickness direction of the eccentric positioning member 121 is the radial direction of the accommodating cavity 1.

[0066] Taking Figure 3 the six eccentric positioning members 121 as an example, the six eccentric positioning members 121 are divided into four groups from near to far relative to the diluent opening 201. One eccentric positioning member 121 closest to the diluent opening 201 is alone in the first group, and one eccentric positioning member 121 farthest from the diluent opening 201 is also alone in the fourth group. The four eccentric positioning members 121 between the one eccentric positioning member 121 closest to the diluent opening 201 and the one eccentric positioning member 121 farthest from the diluent opening 201 are divided into the second group and the third group. The second group and the third group each have two eccentric positioning members 121 with the same radial thickness. Among them, the radial thicknesses of the eccentric positioning members 121 in the first group, the second group, the third group, and the fourth group increase in sequence.

[0067] In this embodiment, in this embodiment, the disk 100 may include a disk body 101 and a cover plate 102, the disk body 101 is the main structure of the disk 100, a groove body is provided on one side of the disk body 101, and the cover plate 102 can be fixed on the disk body 101 by bonding, heat sealing, etc.

[0068] The accommodating chamber 1 , the diluent chamber 2 , the diluent quantitative chamber 3 , the sample injection chamber 4 , the sample liquid quantitative chamber 5 , the mixing chamber 6 , the detection hole 7 and the capillary are all arranged on the disc body 101 .

[0069] The diluent package 200 can be installed in the accommodating chamber 1 from one end of the cover plate 102 along the circumference of the accommodating chamber 1 , and then the cover plate 102 is fixed on the disc body 101 to install the diluent package 200 in the accommodating chamber 1 .

[0070] In this embodiment, the cover plate 102 is provided with a puncture portion 103, which is a pointed structure and is arranged near the diluent outlet 11. The end surface of the cover plate 102 facing the diluent package 200 is a puncturable film. The bottom wall of the accommodating chamber 1 facing away from the cover plate 102 is also provided with a through hole. During detection, the top rod passes through the through hole of the bottom wall of the accommodating chamber 1 and extends into the accommodating chamber 1 to abut against the bottom of the diluent package 200, and drives the diluent package 200 to move upward. After the film at the upper end of the diluent package 200 contacts the puncture portion 103 of the cover plate 102, it is punctured to form a diluent opening 201, and the diluent flows out from the diluent opening 201.

[0071] In other embodiments, the cover plate 102 may not be provided with the piercing portion 103, and the diluent package 200 may be provided with a diluent opening 201 that can be uncovered. Before the diluent package 200 is installed in the accommodating chamber 1, the diluent opening 201 may be manually uncovered to allow the diluent in the diluent package 200 to flow out.

[0072] In this embodiment, since the disc 100 is provided with an eccentric structure 12, the eccentric structure 12 makes the central axis B of the diluent package 200 closer to the diluent opening 201 than the rotation axis A, that is, the eccentric structure 12 makes the diluent package 200 eccentrically arranged relative to the rotation axis A, and the diluent opening 201 of the diluent package 200 is far away from the rotation axis A, so that when the disc 100 is centrifugally rotated, under the action of centrifugal force, the diluent in the eccentric diluent package 200 will centrifugally move toward the diluent opening 201, so that the diluent in the diluent package 200 is completely released; during the process of rotation, acceleration and deceleration, left and right swinging, etc. of the disc 100, no secondary release of the diluent will occur, which can ensure the dilution concentration of the sample, and further ensure the accuracy of the test result.

[0073] In one embodiment, the eccentric structure 12 is only used to make the central axis B of the diluent package 200 closer to the diluent opening 201 than the rotation axis A, that is, the eccentric structure 12 is only used to eccentrically arrange the diluent package 200, and the diluent package 200 can be installed in the accommodation cavity 1 through other structures.

[0074] Please refer to Figure 5 , wherein, the eccentric structure 12 can be a protrusion arranged on the bottom wall and / or the fixed wall of the accommodation cavity 1, and corresponding grooves are provided on the top surface and / or the bottom surface of the diluent package 200. The protruding eccentric structure 12 is connected to the groove of the diluent package 200 to arrange the diluent package 200 at a position eccentric to the rotation axis A.

[0075] Please refer to Figure 6 , the eccentric structure 12 can also be other structures. For example, the eccentric structure 12 has a sticker, and the sticker is installed on the bottom wall and / or the fixed wall of the accommodation cavity 1. The sticker bonds the top surface and / or the bottom surface of the diluent package 200, and can also arrange the diluent package 200 at a position eccentric to the rotation axis A.

[0076] Please refer to Figure 7 , the eccentric structure 12 can also be arranged outside the accommodation cavity 1. For example, the eccentric structure 12 is a magnetic structure such as a magnet. The eccentric structure 12 is arranged outside the accommodation cavity 1 and close to the diluent outlet 11. A corresponding magnet or a magnetizable structure is provided on the diluent package 200. The eccentric structure 12 magnetically adsorbs the diluent package 200 to arrange the diluent package 200 at a position eccentric to the rotation axis A.

[0077] In one embodiment, the eccentric structure 12 can also include one or more eccentric positioning members 121. One or more eccentric positioning members 121 and a part of the side wall of the accommodation cavity 1 enclose a circumference, that is, one or more eccentric positioning members 121 are arranged on the circumferential side surface of the diluent package 200. One or more eccentric positioning members 121 are arranged to wrap the circumferential part of the diluent package 200. One or more eccentric positioning members 121 and the side wall of the accommodation cavity 1 are combined to form an eccentric setting and positioning installation for the diluent package 200.

[0078] Please refer to Figure 8 , the eccentric structure 12 includes an eccentric positioning member 121. The eccentric positioning member 121 is arranged on the side wall of the accommodation cavity 1 and is located at a position relatively far from the diluent outlet 11. The eccentric positioning member 121 presses one side of the diluent package 200, and the side wall of the accommodation cavity 1 presses the other side of the diluent package 200. The eccentric positioning member 121 and the side wall of the accommodation cavity 1 clamp and fix the diluent package, and can also realize the eccentric setting and positioning installation of the diluent package 200.

[0079] In one embodiment, the eccentric positioning member 121 is a rib structure. The eccentric positioning member 121 has an eccentric positioning surface 122, which can be a plane or a curved surface. The area of the eccentric positioning surface 122 gradually increases along the direction in which the diluent package 200 is inserted into the accommodation cavity 1, that is, the eccentric positioning surface 122 gradually increases in area from the upper end (the end close to the cover plate 102) to the lower end of the accommodation cavity 1.

[0080] With such a setting, when the diluent package 200 is inserted into the accommodation cavity 1, the eccentric positioning surface 122 with a smaller area at the upper end has a smaller frictional force with the diluent package 200, which can facilitate the insertion of the diluent package 200 and play a role in guiding the installation; after the diluent package 200 is installed in place downward, it contacts the eccentric positioning surface 122 with a larger area at the lower end, which can increase the frictional force between the eccentric positioning surface 122 and the diluent package 200, and further improve the stability of the fixed installation of the diluent package 200.

[0081] The above uses specific examples to elaborate on the present invention, which is only used to help understand the present invention and is not intended to limit the present invention. For those skilled in the technical field to which the present invention belongs, according to the idea of the present invention, several simple deductions, deformations or substitutions can also be made.

Claims

1. A microfluidic chip, characterized in that: The invention comprises a disk (100) and a diluent bag (200), wherein the disk (100) is provided with a containing cavity (1) and a diluent cavity (2), wherein the containing cavity (1) is located in the middle of the disk (100), and the rotation axis of the disk (100) passes through the containing cavity (1); The accommodating chamber (1) is provided with a diluent outlet (11) which is in communication with the diluent chamber (2); The diluent package (200) has a diluent opening (201), and the diluent package (200) is arranged in the accommodating cavity (1); The disc (100) is provided with an eccentric structure (12), and the eccentric structure (12) is such that the central axis of the diluent bag (200) is closer to the diluent opening (201) than the rotation axis.

2. The microfluidic chip according to claim 1, characterized in that: The eccentric structure (12) is arranged in the accommodating cavity (1).

3. The microfluidic chip according to claim 2, characterized in that: The eccentric structure (12) positions the diluent package (200) in the accommodating chamber (1).

4. The microfluidic chip according to claim 3, characterized in that: The eccentric structure (12) comprises one or more eccentric positioning members (121), wherein the eccentric positioning members (121) press the circumferential side surface of the diluent package (200) so as to bring the central axis of the diluent package (200) closer to the diluent opening (201) than the rotation axis, and to position the diluent package (200) in the accommodating chamber (1).

5. The microfluidic chip according to claim 4, characterized in that: The plurality of eccentric positioning members (121) are distributed around the diluent package (200), and the plurality of eccentric positioning members (121) clamp and position the diluent package (200) in the accommodating cavity (1).

6. The microfluidic chip according to claim 4, characterized in that: One or more of the eccentric positioning members (121) are distributed on the circumferential side surface of the diluent package (200), and one or more of the eccentric positioning members (121) and the side wall of the accommodating chamber (1) clamp and position the diluent package (200) in the accommodating chamber (1).

7. The microfluidic chip according to claim 4, characterized in that: Among the plurality of eccentric positioning members (121), the eccentric positioning member (121) that is farther away from the diluent opening (201) has a thicker thickness along a direction perpendicular to the rotation axis.

8. The microfluidic chip according to claim 4, characterized in that: The eccentric positioning member (121) has an eccentric positioning surface, and the eccentric positioning surface is used to press the circumferential side surface of the diluent package (200).

9. The microfluidic chip according to claim 8, characterized in that: The area of ​​the eccentric positioning surface gradually increases along the direction in which the diluent package (200) is loaded into the accommodating chamber (1).

10. The microfluidic chip according to any one of claims 1 to 9, characterized in that: The accommodating cavity (1) is a hollow cylindrical cavity, and the central axis of the hollow cylindrical cavity coincides with the rotation axis.

11. The microfluidic chip according to any one of claims 1 to 9, characterized in that: The outer surface of the diluent bag (200) is cylindrical.

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