Centrifugal tube capable of preventing aspiration
By setting a wedge-shaped inclined limiter in the inner cavity of the centrifugal tube, the problem that existing centrifugal tubes easily absorb the lower organic reagent when absorbing the upper liquid is absorbed, the extraction efficiency is improved, the liquid loss is reduced, and the adaptation of different liquids is achieved.
Patent Information
- Application Number
- CN202421920790.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-09
- Publication Date
- 2025-06-17
- Estimated Expiration
- 2034-08-09
AI Technical Summary
Existing centrifuge tubes are prone to absorb the lower organic reagents when absorbing the upper liquid, resulting in low extraction efficiency and large liquid loss, mainly due to the lack of targeted anti-absorbing design.
A centrifugal tube that is anti-sucking is designed. By setting an inner tube in the inner cavity of the centrifugal tube body and a limiting member with a wedge-shaped inclined surface is provided at the top of the inner tube, the straw is abutting on the inclined surface of the limiting member when it is exploring down, avoiding touching the underlying organic layer.
Effectively prevent the straw from touching the organic layer below, improve the extraction efficiency of the upper water phase, reduce the loss of the water phase, and realize the adaptation of different centrifugal liquids through the removable connection and the use of different size limits.
Smart Images
Figure CN222984399U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of centrifuge tubes, in particular to a centrifuge tube capable of preventing accidental aspiration. Background Art
[0002] Centrifuge tubes are an important type of consumables in biological research. Most centrifuge tubes are designed with a scaled exterior and a smooth interior, which is a very versatile design. However, in the scenario where stratification occurs after centrifugation of the contents, it is key to the experiment to avoid mixing of the lower layer reagents when aspirating the upper liquid phase. The current general-purpose centrifuge tubes cannot complete this operation efficiently and with high quality. The main reason is that there is no targeted anti-misaspiration design in the existing centrifuge tubes, resulting in the efficiency and results of such operations mainly depending on the skills and proficiency of the operator. In the experimental processes of DNA extraction and purification, RNA extraction and purification, plasmid extraction and purification, etc., the process of organic reagent treatment is often involved, and impurities are removed by centrifugation and stratification.
[0003] In this type of experiment, if the current universal centrifuge tube is used, it is easy to mistakenly aspirate the lower organic phase when aspirating the upper aqueous phase. In order to avoid mixing of the lower reagent when aspirating the upper aqueous phase, the operator usually needs to reduce the operation speed and discard more aqueous phase. Utility Model Content
[0004] The present application provides a centrifuge tube that prevents accidental aspiration, which is used to solve the technical problem that liquid in the centrifuge tube is easily aspirated by mistake when being sucked by a straw.
[0005] The present application provides a centrifuge tube for preventing accidental aspiration, comprising: a centrifuge tube body provided with an inner cavity and an inner tube sleeved in the inner cavity; the inner tube is arranged in close contact with the inner wall of the centrifuge tube body; a plurality of limiters are arranged at one end of the inner tube close to the tube mouth of the centrifuge tube body; the plurality of limiters are all arranged in close contact with the inner wall of the centrifuge tube body, the plurality of limiters are all protruding relative to the inner wall of the centrifuge tube body, the plurality of limiters are all provided with inclined surfaces, the cross-sections of the inclined surfaces are all wedge-shaped, and the angles between the plurality of inclined surfaces and the inner wall close to the tube mouth are all obtuse angles.
[0006] Preferably, there is one limiting member, and the limiting member is ring-arranged at one end of the inner tube.
[0007] Preferably, the centrifuge tube body, the inner tube and the limiting member are integrally formed.
[0008] By adopting the above technical solution, an inner tube is arranged in the inner cavity of the centrifuge tube body, and a limiter with a wedge-shaped inclined surface is arranged at the top of the inner tube, so that the straw rests on the inclined surface of the limiter when it moves down along the inner wall, effectively preventing the straw from touching the organic layer below and safely absorbing the liquid above the limiter, thereby improving the extraction efficiency of the upper aqueous phase and reducing the loss of the aqueous phase.
[0009] Preferably, the inner tube is detachably connected to the centrifuge tube body.
[0010] Preferably, the limiting member is detachably connected to one end of the inner tube close to the centrifuge tube body.
[0011] Preferably, a plurality of grooves are provided on one end of the inner tube close to the centrifuge tube body, and a protrusion structure suitable for embedding into the groove is provided on one end of the stopper close to the inner tube.
[0012] By adopting the above technical solution, the inner tube and the centrifuge tube body are connected in a detachable manner, which is convenient for replacement and cleaning of the inner tube. At the same time, the limiter is detachably arranged on the inner tube. By replacing the limiters of different sizes, adaptation to different centrifugal liquids is achieved, thereby improving the applicability of the equipment.
[0013] Preferably, the distance between the wedge-shaped inclined surfaces between the respective limiting members and the tube opening end of the core tube body is progressive in a step-like manner.
[0014] By adopting the above technical solution, a plurality of limit members with step-by-step increasing heights are arranged on the inner tube, so that when facing different centrifugal solutions, the limit member above the boundary layer can be selected for abutment according to the position of the boundary layer after centrifugation, thereby achieving the adaptation of the equipment to different solutions and improving the practicability of the equipment.
[0015] One or more technical solutions provided in this application have at least the following technical effects or advantages:
[0016] 1. An inner tube is arranged in the inner cavity of the centrifuge tube body, and a stopper with a wedge-shaped inclined surface is arranged at the top of the inner tube, so that the pipette is pressed against the inclined surface of the stopper when probing down along the inner wall, effectively preventing the pipette from touching the organic layer below and safely absorbing the liquid above the stopper, thereby improving the extraction efficiency of the upper aqueous phase and reducing the loss of the aqueous phase;
[0017] 2. The centrifuge tube body and the inner tube are integrally formed. During production, a wedge-shaped stopper is generated by the gradient change of the centrifuge tube wall thickness. At the same time, the integrally formed setting facilitates batch production;
[0018] 3. The inner tube and the centrifuge tube body are connected in a detachable manner to facilitate replacement and cleaning of the inner tube. At the same time, the limiter is detachably arranged on the inner tube. By replacing the limiters of different sizes, adaptation to different centrifugal liquids is achieved, thereby improving the applicability of the equipment;
[0019] 4. A plurality of limiters with increasing heights in a step-by-step manner are arranged on the inner tube, so that when facing different centrifugal solutions, the limiter above the boundary layer can be selected for abutment according to the position of the boundary layer after centrifugation, thereby realizing the adaptation of the equipment to different solutions and improving the practicability of the equipment. BRIEF DESCRIPTION OF THE DRAWINGS
[0020] To more clearly illustrate the technical solutions in the embodiments of the present utility model, the following will briefly introduce the attached drawings required for the description of the embodiments. Obviously, the attached drawings in the following description are only some embodiments of the present utility model. For those of ordinary skill in the art, without creative efforts, other attached drawings can also be obtained based on these drawings.
[0021] Figure 1 One of the cross-sectional views of a centrifuge tube for preventing accidental aspiration provided by this application;
[0022] Figure 2 Another cross-sectional view of a centrifuge tube for preventing accidental aspiration provided by this application;
[0023] Figure 3 An axonometric view of the inner tube and the limiting member of a centrifuge tube for preventing accidental aspiration provided by this application;
[0024] Figure 4 An axonometric view of the inner tube of a centrifuge tube for preventing accidental aspiration provided by this application.
[0025] Explanation of reference numerals: 1. Centrifuge tube body; 11. Inner cavity; 12. Inner wall; 2. Inner tube; 21. Groove; 3. Limiting member; 31. Inclined surface; 32. Protrusion structure; 4. Aqueous solution layer; 5. Interface layer; 6. Organic layer; 7. Pipette. Specific embodiments
[0026] This application provides a centrifuge tube for preventing accidental aspiration, which is used to solve the technical problem that it is easy to accidentally aspirate the liquid in the centrifuge tube when using a pipette in the prior art.
[0027] The following will clearly and completely describe the technical solutions in the embodiments of this application with reference to the attached drawings in the embodiments of this application. Obviously, the described embodiments are only some embodiments of this application, rather than all embodiments. Based on the embodiments in this application, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of this application.
[0028] It should be noted that the terms "first", "second", etc. in the specification and claims of the present application and the above-mentioned drawings are used to distinguish similar objects, and are not necessarily used to describe a specific order or sequence. It should be understood that the data used in this way can be interchangeable where appropriate, so that the embodiments of the present application described herein can be implemented in an order other than those illustrated or described herein. In addition, the terms "including" and "having" and any variations thereof are intended to cover non-exclusive inclusions, for example, a process, method, system, product, or server that includes a series of steps or units is not necessarily limited to those steps or units that are clearly listed, but may include other steps or modules that are not clearly listed or inherent to these processes, methods, products, or devices.
[0029] Example 1
[0030] like Figures 1 to 4 The embodiment of the present application shown provides a centrifuge tube for preventing accidental aspiration, comprising: a centrifuge tube body 1 provided with an inner cavity 11 and an inner tube 2 sleeved in the inner cavity 11; the inner tube 2 is arranged in a close contact with the inner wall 12 of the centrifuge tube body 1; a plurality of limit members 3 are provided at one end of the inner tube 2 close to the tube mouth of the centrifuge tube body 1; the plurality of limit members 3 are all arranged in a close contact with the inner wall 12 of the centrifuge tube body 1, the plurality of limit members 3 are all protruding relative to the inner wall 12 of the centrifuge tube body 1, the plurality of limit members 3 are all provided with wedge-shaped inclined surfaces 31, and the angles between the plurality of wedge-shaped inclined surfaces 31 and the inner wall 12 close to the tube mouth are all obtuse angles.
[0031] More preferably, in the embodiments provided in this application, Figure 1 As shown, an integrally formed inner tube 2 is provided in the inner cavity 11 of the centrifuge tube body 1, and the thickness of the inner tube 2 is the same as that of the centrifuge tube body 1. The inner tube 2 covers the inner wall 12 at the bottom of the inner cavity 11, wherein the distance between the top end of the inner tube 2 and the tube mouth of the core tube body 1 can be adjusted to different specifications when producing the centrifuge tube. In this embodiment, the distance between the upper end of the inner tube 2 and the tube mouth of the core tube body 1 is half the length of the centrifuge tube body 1, and a stopper 3 is provided on one end of the inner tube 2 near the tube mouth. The stopper 3 is arranged to fit the inner wall 12 of the centrifuge tube body 1, and the portion of the stopper 3 facing the inner cavity 11 is a circle of inclined surfaces 31, and the cross-sectional shape of the inclined surfaces 31 is wedge-shaped, and the angle between the inclined surfaces 31 and the inner wall 12 near the tube mouth is an obtuse angle of 135°. The stopper 3 is integrally formed with the inner tube 2 and the centrifuge tube body 1, and the distance between the upper inclined surface 31 of the stopper 3 and the tube mouth of the centrifuge tube body 1 can be adjusted to different specifications by changing the gradient of the centrifuge tube thickness during production, and the integral molding setting facilitates mass production.
[0032] During use, according to the type of centrifuged liquid, a centrifuge tube body 1 with a limiting member 3 of appropriate height is selected, such that the limiting member 3 is located above the organic layer 6. After centrifugation, the organic layer 6 is located below the limiting member 3 in the inner cavity 11, and the limiting member 3 is located in the aqueous solution layer 4 above the demarcation layer 5. After centrifugation, the suction port of the pipette 7 extends downward along the inner wall 12 of the centrifuge tube body 1 until it abuts against the inclined surface 31 of the limiting member 3. Taking the extraction of RNA using Trizol as an example, 1 mL of Trizol is added with 200 μL of chloroform, mixed well and centrifuged for stratification. The volume of the lower organic phase is about 700 μL. Therefore, in one embodiment, the position of the limiting structure is set at 750 μL of the centrifuge tube.
[0033] In this embodiment, by arranging an inner tube 2 in the inner cavity 11 of the centrifuge tube body 1 and arranging a limiting member 3 with a wedge-shaped inclined surface 31 at the top of the inner tube 2, when the pipette 7 extends downward along the inner wall 12, it abuts against the inclined surface 31 of the limiting member 3, effectively preventing the pipette 7 from touching the lower organic layer 6 and safely sucking the liquid above the limiting member 3, improving the extraction efficiency of the upper aqueous phase and reducing the loss of the aqueous phase.
[0034] Embodiment 2
[0035] Further, on the basis of the above embodiment, as Figures 2 to 4 shown, the inner tube 2 and the centrifuge tube body 1 are detachably connected. The outer shape of the inner tube 2 fits the bottom of the inner cavity 11 of the centrifuge tube body 1. The inner tube 2 is sleeved into the inner cavity 11 along the inner wall 12 of the centrifuge tube body 1, and the limiting member 3 and the inner tube 2 are detachably connected.
[0036] In this embodiment, four grooves 21 with the same shape are evenly arranged on the outer wall of the inner tube 2, and protruding structures 32 adapted to be embedded in the grooves 21 are arranged on all four limiting members 3; during use, the limiting member 3 is installed on the inner tube 2 through the cooperation of the protruding structure 32 and the groove 21, and then the inner tube 2 is installed in the inner cavity 11 of the centrifuge tube body 1.
[0037] In this embodiment, by detachably connecting the inner tube 2 and the centrifuge tube body 1, it is convenient to replace and clean the inner tube 2. At the same time, the limiting member 3 is detachably arranged on the inner tube 2. By replacing the limiting members 3 of different sizes, the adaptation to different centrifuged liquids is realized, and the applicability of the equipment is improved.
[0038] Embodiment 3,
[0039] Further, on the basis of Embodiment 2, as Figure 3As shown, four limiting members 3 are detachably provided on the inner tube 2. The four limiting members 3 are combined into a circle, and the heights of the four limiting members 3 increase in sequence. During use, after centrifugation to complete liquid stratification, according to the position of the demarcation layer 5, the straw 7 is abutted against the inclined surface 31 of the limiting member 3 above the demarcation layer 5 to draw the aqueous phase.
[0040] In this embodiment, by providing a plurality of limiting members 3 on the inner tube 2 with stepped increasing heights, when facing different centrifuged solutions, it is possible to select the limiting member 3 above the demarcation layer 5 for abutment according to the position of the demarcation layer 5 after centrifugation, realizing the adaptation of the device to different solutions and improving the practicability of the device.
[0041] It should be noted that the above sequence of embodiments of the present application is only for description and does not represent the superiority or inferiority of the embodiments. And the above specific embodiments of this specification have been described. Other embodiments are within the scope of the appended claims. In some cases, the actions or steps recited in the claims can be executed in a different order from that in the embodiments and still achieve the desired result. Additionally, the processes depicted in the drawings do not necessarily require the specific order or sequential order shown to achieve the desired result. In certain embodiments, multitasking and parallel processing are also possible or may be advantageous.
[0042] The above are only the preferred embodiments of the present application and are not intended to limit the present application. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.
[0043] This specification and the drawings are only exemplary descriptions of the present application and are considered to have covered any and all modifications, variations, combinations, or equivalents within the scope of the present application. Obviously, those skilled in the art can make various changes and modifications to the present application without departing from the scope of the present application. Thus, if these modifications and variations of the present application fall within the scope of the present application and its equivalent technologies, the present application is intended to include these changes and modifications.
Claims
1. A centrifuge tube for preventing accidental aspiration, characterized in that: The invention comprises a centrifuge tube body (1) provided with an inner cavity (11) and an inner tube (2) sleeved in the inner cavity (11); the inner tube (2) is arranged in close contact with the inner wall (12) of the centrifuge tube body (1); a plurality of stoppers (3) are arranged at one end of the inner tube (2) close to the tube mouth of the centrifuge tube body (1); the plurality of stoppers (3) are all arranged in close contact with the inner wall (12) of the centrifuge tube body (1), the plurality of stoppers (3) are all protruding relative to the inner wall (12) of the centrifuge tube body (1), the plurality of stoppers (3) are all provided with inclined surfaces (31), the cross sections of the inclined surfaces (31) are all wedge-shaped, and the angles between the plurality of inclined surfaces (31) and the inner wall (12) close to the tube mouth are all obtuse angles.
2. A centrifuge tube for preventing accidental aspiration according to claim 1, characterized in that: The number of the limiting member (3) is one, and the limiting member (3) is arranged around one end of the inner tube (2).
3. A centrifuge tube for preventing accidental aspiration as claimed in claim 2, characterized in that: The centrifuge tube body (1), the inner tube (2) and the limiting member (3) are integrally formed.
4. A centrifuge tube for preventing accidental aspiration according to claim 1, characterized in that: The inner tube (2) is connected to the centrifuge tube body (1) in a detachable manner.
5. A centrifuge tube for preventing accidental aspiration as claimed in claim 4, characterized in that: The limiting member (3) is detachably connected to one end of the inner tube (2) close to the centrifuge tube body (1).
6. A centrifuge tube for preventing accidental aspiration according to claim 5, characterized in that: A plurality of grooves (21) are provided on one end of the inner tube (2) close to the centrifuge tube body (1), and a protruding structure (32) suitable for being embedded in the grooves (21) is provided on one end of the stopper (3) close to the inner tube (2).
7. A centrifuge tube for preventing accidental aspiration according to claim 6, characterized in that: The distance between the wedge-shaped inclined surfaces (31) between the respective stoppers (3) and the tube opening end of the core tube body (1) is progressive in a step-like manner.