Automatic enzyme inhibition ratio method pesticide residue detection device

Abstract

The utility model discloses an automatic enzyme inhibition ratio method pesticide residue detection device which comprises a vibration separation assembly, a liquid adding and transferring assembly, a constant temperature shaking assembly and a spectrum detection assembly, the vibration separation assembly is used for preparing a sample extracting solution, the sample extracting solution is transferred to the constant temperature shaking assembly through the liquid adding and transferring assembly, then enzyme and a color developing agent are added, and the spectrum detection assembly is used for detecting the pesticide residue. And after constant-temperature culture is completed, transferring to a spectrum detection assembly for detection. After a sample is placed in the device, the preparation of a sample extracting solution is automatically completed; enzyme adding, color developing agent adding and constant-temperature culture operations of a sample extracting solution are automatically completed; and the spectral analysis operation of the sample extracting solution is automatically completed.

Description

Technical Field

[0001] The utility model relates to the field of food safety detection, in particular to an automated enzyme inhibition rate method pesticide residue detection device. Background Art

[0002] Pesticide residues are one of the causes of food poisoning, so it is necessary to conduct targeted testing; under certain conditions, organophosphorus and carbamate pesticides have an inhibitory effect on the normal function of cholinesterase, and the inhibition rate is positively correlated with the concentration of the pesticide. Under normal circumstances, the enzyme catalyzes the hydrolysis of the neurotransmitter (acetylcholine), and its hydrolysis product reacts with the color developer to produce a yellow substance. The change in absorbance over time at 412nm is measured with a spectrophotometer to calculate the inhibition rate. The inhibition rate can be used to determine whether there is a high dose of organophosphorus or carbamate pesticides in the sample.

[0003] An existing detection process is as follows: 1. Take representative vegetables and remove the soil. For leafy vegetables, take the leaf part and weigh 3g on a balance, and for fruits, weigh 6g; 2. Cut the vegetable sample into 1 cm squares and place them in a small beaker, add 10ml of buffer reagent, and shake for 1 to 2 minutes or more than 50 times; 3. Filter it (or pour it into another container and let it stand for 2 to 5 minutes and then take the supernatant) to obtain the sample extract; 4. Take an appropriate number of test tubes, add 2.5ml of buffer reagent to one of them as a blank control, and add 2.5ml of the sample solution to be tested to the rest, and then add in sequence: enzyme → color developer → shake well → constant temperature culture for 15 minutes → obtain substrate, and after further shaking, immediately pour it into a colorimetric dish, then put it into the instrument and perform detection on the machine.

[0004] There are a large number of agricultural products to be tested, and the above process is time-consuming and labor-intensive to operate manually. Therefore, a device that can automatically complete most of the above operations is urgently needed. Utility Model Content

[0005] The utility model aims to solve one of the technical problems in the above-mentioned technology at least to a certain extent. To this end, the purpose of the utility model is to provide a device that can automatically realize the pesticide residue detection process.

[0006] In order to achieve the above-mentioned purpose, the embodiment of the utility model proposes an automated enzyme inhibition rate method pesticide residue detection device, comprising:

[0007] Oscillation separation component: It consists of an oscillation chassis and an oscillation bracket. The oscillation bracket is movably provided with an oscillation container. The sample is placed in the oscillation container, and the oscillation container is placed in the oscillation bracket. The oscillation chassis drives the oscillation bracket to shake in a plane to produce a sample extract.

[0008] Liquid addition and pipetting assembly: It includes a three-axis pipetting bracket, a pipette tip storage bracket, and a waste pipette tip collection element; the three-axis pipetting bracket is provided with a pipettor and a liquid addition element. The pipettor moves on the three-axis pipetting bracket, assembles a pipette tip from the pipette tip storage bracket, then moves to the oscillation container to extract the sample extract. After the operation is completed, the used pipette tip is unloaded into the waste pipette tip collection element;

[0009] Constant temperature shaking assembly: It includes a shaking element and a constant temperature element. The shaking element is provided with a number of shaking fixing grooves, and the constant temperature element is arranged on the inner wall of the shaking fixing groove. A test tube is inserted into the shaking fixing groove. The pipettor injects the sample extract into the test tube, the shaking element moves, and the constant temperature element presses against the test tube to heat and keep warm for a set time;

[0010] Spectral detection assembly: It includes a spectral element and a detection box. The pipettor transfers the sample extract from the constant temperature shaking assembly to the detection box, and the spectral element performs detection through the detection box;

[0011] The liquid addition element moves to various positions on the three-axis pipetting bracket to add corresponding detection reagents.

[0012] The automatic pesticide residue detection device based on the enzyme inhibition rate method according to the embodiment of the present invention has at least the following beneficial effects:

[0013] 1. After putting in the sample, the production of the sample extract is automatically completed;

[0014] 2. The operations of adding enzyme, adding chromogenic agent, and constant temperature culture to the sample extract are automatically completed;

[0015] 3. The spectral analysis operation of the sample extract is automatically completed.

[0016] In addition, an automatic pesticide residue detection device based on the enzyme inhibition rate method proposed according to the above embodiment of the present invention may also have the following additional technical features:

[0017] Optionally, the oscillation chassis includes an oscillation motor and an oscillation sliding member. The oscillation sliding member has a structure of two-dimensional translation in a plane. The oscillation motor is connected to the oscillation sliding member to drive the oscillation sliding member to move.

[0018] Further, an oscillation bracket is fixed on the oscillation sliding member. The oscillation bracket is provided with a number of oscillation vertical holes perpendicular to the plane of movement of the oscillation sliding member. The oscillation container is inserted into the oscillation vertical holes, and an oscillation fixing element surrounded by a number of springs is provided at the orifice of the oscillation vertical holes to fix the oscillation container.

[0019] Optionally, the three-axis pipetting bracket includes three mutually perpendicular sliding pairs. On one of the sliding pairs perpendicular to the bottom surface, there are a pipette mounting seat and a liquid addition head mounting seat. The pipette mounting seat is arranged on the sliding block of the sliding pair and moves up and down with the sliding block; the liquid addition head mounting seat is arranged on the guide rail of the sliding pair and moves with the overall movement of the sliding pair.

[0020] Optionally, the pipette tip storage bracket includes a pipette tip loading rack and a loading rack fixing clip. The pipette tip loading rack vertically stores the pipette tips. After the pipette tips are used up, the loading fixing clip releases the pipette tip loading rack. After replenishing the pipette tips, the loading fixing clip fixes it again.

[0021] Optionally, the test box includes a test tube and a test tube bracket for storing the test tube. The test tube is stored in the test tube bracket, and a spectral element is arranged inside the test tube bracket. The pipette injects the substrate into the test tube, and the value is measured through the spectral element. Description of the Drawings

[0022] Figure 1 It is an overall structural view according to an embodiment of the present invention;

[0023] Figure 2 It is a partial structural view according to an embodiment of the present invention;

[0024] Figure 3 It is a structural view of the liquid addition and pipetting assembly according to an embodiment of the present invention;

[0025] Figure 4 It is an exploded view of the oscillation separation assembly from one angle according to an embodiment of the present invention;

[0026] Figure 5 It is an exploded view of the oscillation separation assembly from another angle according to an embodiment of the present invention;

[0027] Figure 6 It is an exploded view of the constant temperature shaking and mixing assembly from one angle according to an embodiment of the present invention;

[0028] Figure 7 It is an exploded view of the constant temperature shaking and mixing assembly from another angle according to an embodiment of the present invention;

[0029] Figure 8 It is a structural view of the spectral detection assembly according to an embodiment of the present invention.

[0030] Reference Signs Explanation:

[0031] Oscillation separation assembly 1

[0032] Oscillation chassis 11, Oscillation motor 111, Oscillation slider 112

[0033] Oscillation bracket 12, oscillation fixing element 121, oscillation container 13

[0034] Liquid adding and pipetting assembly 2

[0035] Three - axis pipetting bracket 21, pipette mounting seat 211, liquid adding head mounting seat 212

[0036] Pipette tip storage bracket 22, pipette tip loading rack 221, loading rack fixing clip 222

[0037] Waste pipette tip collection element 23

[0038] Constant - temperature shaking component 3

[0039] Shaking element 31, constant - temperature element 32

[0040] Spectral detection component 4

[0041] Spectral element 41, detection box 42. Detailed implementation mode

[0042] The following will describe in detail the embodiments of the present utility model. The examples of the embodiments are shown in the drawings, where the same or similar reference numerals represent the same or similar elements or elements with the same or similar functions throughout. The embodiments described below with reference to the drawings are exemplary and are intended to explain the present utility model, and should not be construed as a limitation of the present utility model.

[0043] The oscillation separation component of the present utility model is used to prepare a sample extraction solution. The sample extraction solution is transferred to the constant - temperature shaking component through the liquid adding and pipetting assembly, and then enzymes and color - developing agents are added, and constant - temperature culture is completed, and then it is transferred to the spectral detection component for detection.

[0044] To better understand the above - mentioned technical solution, the exemplary embodiments of the present utility model will be described in more detail with reference to the drawings. Although the exemplary embodiments of the present utility model are shown in the drawings, it should be understood that the present utility model can be implemented in various forms and should not be limited by the embodiments described herein. On the contrary, these embodiments are provided to enable a more thorough understanding of the present utility model and to fully convey the scope of the present utility model to those skilled in the art.

[0045] To better understand the above - mentioned technical solution, the above - mentioned technical solution will be described in detail in combination with the specification drawings and specific implementation modes.

[0046] The enzyme inhibition method is a detection method that utilizes the phenomenon that the functional groups of an enzyme are affected by a certain substance, resulting in a decrease or loss of enzyme activity. This substance is called an enzyme inhibitor. Enzyme inhibitors are selective for enzymes and are important tools for studying the mechanism of enzyme action. In addition, there are also some biological macromolecules with certain functions existing in animals and plants that are enzyme inhibitors. It is mainly used for the detection of pesticide residues.

[0047] Figures 1 to 7 An automated pesticide residue detection device based on the enzyme inhibition rate method according to an embodiment of the present invention includes:

[0048] Oscillation separation component 1: It consists of an oscillation chassis 11 and an oscillation bracket 12. An oscillation container 13 is movably arranged on the oscillation bracket 12. The test sample is placed in the oscillation container 13, and the oscillation container 13 is placed on the oscillation bracket 12. The oscillation chassis 11 drives the vibration bracket to shake in a plane to produce a sample extraction solution. As described in the background art, one object of the present invention is to replace some manual operations in the detection process. When using the present invention, the production of the test sample is completed by manual or other equipment. After the production of the test sample is completed, it is placed in the oscillation container 13, and a buffer reagent is added through the liquid adding component to produce a sample extraction solution. This process can also be manually added with the buffer reagent. Since the operation rhythm of the equipment is fixed, manual intervention in this process can provide a more flexible detection rhythm when needed.

[0049] Specifically, the oscillation chassis 11 includes an oscillation motor 111 and an oscillation slider 112. The oscillation slider 112 is a structure that translates two-dimensionally in a plane. The oscillation motor 111 is connected to the oscillation slider 112 to drive the oscillation slider 112 to move. For the structure of two-dimensional translation, one solution is to use a planar guide rail to form a two-layer structure. In this way, it can move freely along two mutually perpendicular axes in a plane. An eccentric block can be set on the axis of the oscillation motor 111. The setting method can be directly connected through a coupling or connected by a synchronous belt. There is a circular hole in the planar guide rail, and the eccentric block swings inside. Since the axis does not change its position, the eccentric block can drive the guide rail inside the oscillation slider 112 to produce relative movement.

[0050] Furthermore, an oscillation bracket 12 is fixed on the oscillation slider 112. The oscillation bracket 12 is provided with a plurality of oscillation vertical holes perpendicular to the plane of movement of the oscillation slider 112. The oscillation container 13 is inserted into the oscillation vertical holes, and an oscillation fixing element 121 formed by a plurality of springs is provided at the orifice of the oscillation vertical holes to fix the oscillation container 13. By using this fixing method, different oscillation containers 13 within a certain thickness range can be conveniently adapted.

[0051] Liquid addition and pipetting assembly 2: It includes a three-axis pipetting bracket 21, a pipette tip storage bracket 22, and a waste pipette tip collection element 23; The three-axis pipetting bracket 21 is provided with a pipettor and a liquid addition element. The pipettor moves on the three-axis pipetting bracket 21, assembles a pipette tip from the pipette tip storage bracket 22, then moves to the shaking container 13 to extract the sample extract. After the operation is completed, the used pipette tip is unloaded into the waste pipette tip collection element 23; The pipettor is a conventional structure. For the liquid addition element, in one solution, a plurality of injection heads are provided on one element. The injection heads are connected to a pump, the pump is connected to a tube, and the tube is connected to the corresponding container. The start and stop time of the pump is controlled by an external control method to control the amount of liquid added.

[0052] Specifically, the three-axis pipetting bracket 21 includes three mutually perpendicular sliding pairs. On one of the sliding pairs perpendicular to the bottom surface, a pipettor mounting seat 211 and a liquid addition head mounting seat 212 are provided. The pipettor mounting seat 211 is arranged on the sliding block of the sliding pair and moves up and down with the sliding block; The liquid addition head mounting seat 212 is arranged on the guide rail of the sliding pair and moves with the overall movement of the sliding pair.

[0053] Optionally, the pipette tip storage bracket 22 includes a pipette tip loading rack 221 and a loading rack fixing clip 222. The pipette tip loading rack 221 vertically stores the pipette tips. After the pipette tips are used up, the loading fixing clip releases the pipette tip loading rack 221. After replenishing the pipette tips, it is fixed again by the loading fixing clip. The pipette tip storage bracket 22 can be a space frame structure composed of multiple plates and columns. The loading rack fixing clip 222 fixes the pipette tip storage bracket 22 by fixing one of the plates.

[0054] Constant temperature shaking assembly 3: It includes a shaking element 31 and a constant temperature element 32. The shaking element 31 is provided with a number of shaking fixing grooves. The constant temperature element 32 is arranged on the inner wall of the shaking fixing groove. A test tube is inserted into the shaking fixing groove. The pipettor injects the sample extract into the test tube. The shaking element 31 moves, and the constant temperature element 32 closely adheres to the test tube to heat and keep warm for a set time; Specifically, the movement principle of the shaking element 31 is similar to that of the shaking chassis 11.

[0055] Spectral detection assembly 4: It includes a spectral element 41 and a detection box 42. The pipettor transfers the sample extract from the constant temperature shaking assembly 3 into the detection box 42, and the spectral element 41 performs detection through the detection box 42;

[0056] The liquid addition element moves to various positions on the three-axis pipetting bracket 21 to add the corresponding detection reagents.

[0057] Optionally, the detection box 42 includes a detection tube and a detection tube bracket for storing the detection tube. The detection tube is stored in the detection tube bracket. The spectral element 41 is arranged in the detection tube bracket. The pipettor injects the substrate into the detection tube, and the value is measured through the spectral element 41.

[0058] In the description of the present utility model, it should be understood that the orientation or positional relationship indicated by terms such as "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc. is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation to the present utility model.

[0059] In the present utility model, unless otherwise clearly specified and defined, the first feature being "above" or "below" the second feature may include the direct contact between the first and second features, or may include the situation where the first and second features are not in direct contact but in contact through additional features therebetween. Moreover, the first feature being "above", "over" and "on" the second feature includes that the first feature is directly above and obliquely above the second feature, or merely means that the horizontal height of the first feature is higher than that of the second feature. The first feature being "under", "beneath" and "underneath" the second feature includes that the first feature is directly below and obliquely below the second feature, or merely means that the horizontal height of the first feature is lower than that of the second feature.

[0060] In the description of this specification, the description with reference to terms such as "one embodiment", "some embodiments", "example", "specific example", or "some examples", etc. means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present utility model. In this specification, the schematic representation of the above terms should not be understood as necessarily referring to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in a suitable manner in any one or more embodiments or examples. In addition, those skilled in the art can combine and combine the different embodiments or examples described in this specification.

[0061] Although the embodiments of the present utility model have been shown and described above, it can be understood that the above embodiments are exemplary and should not be construed as a limitation to the present utility model. Those of ordinary skill in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of the present utility model.

Claims

1. An automated enzyme inhibition rate method pesticide residue detection device, characterized in that: include Oscillation separation component: It consists of an oscillation chassis and an oscillation bracket. The oscillation bracket is movably provided with an oscillation container. The sample is placed in the oscillation container, and the oscillation container is placed in the oscillation bracket. The oscillation chassis drives the oscillation bracket to shake in a plane to produce a sample extract. Liquid adding and pipetting components: including a three-axis pipetting support, a pipetting head storage support and a waste pipetting head collection element; the three-axis pipetting support is provided with a pipette and a liquid adding element, the pipette moves on the three-axis pipetting support, assembles a pipetting head from the pipetting head storage support, and then moves to the oscillation container to extract the sample extract, and after the operation is completed, unloads the used pipetting head into the waste pipetting head collection element; Constant temperature shaking component: including a shaking element and a constant temperature element. The shaking element is provided with a plurality of shaking fixed grooves. The constant temperature element is arranged on the inner wall of the shaking fixed groove. A test tube is inserted into the shaking fixed groove. The pipette injects the sample extract into the test tube. The shaking element moves and the constant temperature element is close to the test tube to heat and keep it warm until the set time. Spectral detection component: including a spectral element and a detection box. The pipette transfers the sample extract from the constant temperature shaking component to the detection box, and the spectral element performs detection through the detection box; The liquid adding element moves to various positions on the three-axis liquid transfer support to add corresponding detection reagents.

2. The automated enzyme inhibition rate method pesticide residue detection device according to claim 1, characterized in that: The oscillating chassis includes an oscillating motor and an oscillating sliding member. The oscillating sliding member is a structure that translates two-dimensionally in a plane. The oscillating motor is connected to the oscillating sliding member to drive the oscillating sliding member to move.

3. The automated enzyme inhibition rate method pesticide residue detection device according to claim 2, characterized in that: An oscillation bracket is fixed on the oscillation sliding member. The oscillation bracket is provided with a plurality of oscillation vertical holes perpendicular to the plane of movement of the oscillation sliding member. The oscillation container is inserted into the oscillation vertical hole. An oscillation fixing element surrounded by a plurality of springs is provided at the oscillation vertical hole mouth to fix the oscillation container.

4. The automated enzyme inhibition rate method pesticide residue detection device according to claim 1, characterized in that: The three-axis pipetting support includes three mutually perpendicular sliding pairs, one of which is perpendicular to the bottom surface and is provided with a pipette mounting seat and a liquid adding head mounting seat, wherein the pipette mounting seat is arranged on the sliding block of the sliding pair and moves up and down with the sliding block; the liquid adding head mounting seat is arranged on the guide rail of the sliding pair and moves with the overall movement of the sliding pair.

5. The automated enzyme inhibition rate method pesticide residue detection device according to claim 1, characterized in that: The pipette head storage bracket includes a pipette head upper rack and a loading rack fixing clamp. The pipette head upper rack vertically stores the pipette heads. After the pipette heads are used, the loading rack is released by the loading clamp. After the pipette heads are replenished, they are fixed by the loading clamp.

6. The automated enzyme inhibition rate method pesticide residue detection device according to claim 1, characterized in that: The detection box includes a detection tube and a detection tube holder for storing the detection tube. The detection tube is stored in the detection tube holder, and the spectral element is arranged in the detection tube holder. The pipette injects the substrate into the detection tube, and the value is measured by the spectral element.

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