Novel microplate reader based on grating module and optical filter module

Abstract

A novel microplate reader based on a grating module and an optical filter module comprises an electric microplate reader body, the grating module, the optical filter module, a motor reducing mechanism, a detection mechanism, a first detection control circuit and a second detection control circuit, the motor reducing mechanism is mounted at the rear side end in the detection bin, a supporting rod and a connecting rod are mounted at the front side end of a rotating shaft of the motor reducing mechanism, and the rear side ends of the grating module and the optical filter module are mounted together with the two ends of the front side of the connecting rod respectively; the detection mechanism comprises an adjustable resistor and a driven rubber wheel, the driven rubber wheel is installed on an adjusting handle of the adjustable resistor, and the lower portion of the adjustable resistor is installed on one side of a shell of the motor speed reducing mechanism. The first detection control circuit and the second detection control circuit are arranged in an element bin of the microplate reader body and are electrically connected. According to the utility model, convenience is brought to workers, the equipment fund investment of related departments is correspondingly saved, and a better detection effect can be realized on samples. Based on the above, the system has a good application prospect.

Description

Technical Field

[0001] The utility model relates to the technical field of detection equipment, in particular to a novel microplate reader based on a grating module and a filter module. Background Art

[0002] A microplate reader is a very important medical device with great application value and is widely used in many industries such as major hospitals, university research institutions, disease control centers, technical quality supervision bureaus, animal and plant inspection and quarantine, and food and feed. In the application of a microplate reader, the inspector opens the door at the lower front end of the detection chamber, then places the sample to be detected (in a plastic microplate) on the tray inside the detection chamber, and after closing the door, the inspector controls the device to detect the sample by operating the buttons at the upper front end of the housing. Currently, there are many types of microplate readers, including grating type microplate readers, filter type microplate readers, etc. Specifically, the light wave emitted by the light source lamp at the upper end inside the detection chamber of the microplate reader body passes through the grating module or the filter module and enters the specimen to be measured in the plastic microplate. Then, a part of this monochromatic light is absorbed by the specimen, and the other part passes through the specimen and is irradiated onto the photodetector at the lower end inside the microplate reader through the transparent tray. The photodetector inputs the light signals with different intensities according to the different specimens to the signal input end of the conversion module of the microplate reader. The conversion module converts it into corresponding electrical signals, and after signal processing such as pre-amplification, logarithmic amplification, and analog-to-digital conversion, the electrical signals are sent to the microprocessor system for data processing and calculation. Finally, the data results of the detected sample (such as allergens, thyroid, inorganic toxins, etc.) are displayed on the display screen, or printed through an externally configured printer. In actual situations, except for the different core components, the grating module and the filter module (the signal input ends of the grating module and the filter module are both connected to the control signal output end of the microprocessor system through wires), the other components are the same. The grating module and the filter module have their own advantages and disadvantages when applied to a microplate reader, mainly as follows.

[0003] The main function of the grating module is to separate the white light source emitted by the light source lamp through the principle of physical diffraction grating, and to separate the selected excitation wavelength through its own slit and act on the sample. The grating module can intercept any wavelength within the light source wavelength range and has high flexibility. In the specific detection of grating microplate readers, there are the following advantages. (1) It can quickly and accurately detect trace biological molecules, so it has high sensitivity; (2) It can detect very small biological molecules, even biological molecular substances such as proteins and nucleic acids at extremely low concentrations, and the detection accuracy is very high; (3) It can intercept any wavelength within the light source wavelength range, so it has greater flexibility in experimental design. However, due to its own conditions, the microplate reader based on the grating module also has the following disadvantages. (1) High requirements for samples: Specifically, in order to ensure the accuracy and reliability of detection, the grating microplate reader has high requirements for the quality of samples. If the samples are contaminated or of poor quality, it may affect the accuracy of experimental detection results; (2) Relatively limited sensitivity: Although the grating microplate reader has high sensitivity, in some detections with high sensitivity requirements, such as TRF, TR-FRET, etc., its sensitivity is not as good as that of the microplate reader equipped with a filter module. The main function of the filter module in the microplate reader is to selectively transmit or reflect light according to different wavelengths, so as to achieve the separation and enhancement of sample signals. Specifically, the filter module can ensure that only light of a specific wavelength irradiates the sample, so as to effectively excite the sample to generate the required signal, and can select a suitable emission wavelength to detect the fluorescence emitted by the sample or it can chemiluminesce, filtering out other stray light except the required wavelength, and improving the accuracy and sensitivity of detection. In the specific detection of filter microplate readers, there are the following advantages. (1) The filter module has always been the gold standard for high-sensitivity detection since the birth of the microplate reader, with very good light transmittance (can be higher than 90%), and less loss of light energy, which makes the fluorescence signal easy to detect, thus ensuring a high signal-to-noise ratio (SNR); (2) The filter module can accurately select light of a specific wavelength for excitation and detection, avoiding the interference of stray light and improving the accuracy of detection, with the advantage of high precision; (3) It can meet the detection needs of samples with different wavelengths and has good flexibility. However, due to its own conditions, the microplate reader based on the filter module also has the following disadvantages. Specifically, since the wavelength of the filter is fixed, the transmitted wavelength is also fixed, which limits the application of the microplate reader in some sample detection experiments that require continuous wavelength adjustment.

[0004] With the development of industrial technology, the technology of microplate readers has also made certain progress. For example, in the authorized patent with the Chinese patent number "202223304502.2" and the patent name "A dust-proof microplate reader", it is recorded that "This utility model is provided with a sealing mechanism, which can tightly press the sealing ring against the limiting frame near the opening, so that the opening can be effectively sealed. Compared with traditional microplate readers, there will be no gaps between the sealing plate and the microplate reader due to long-term use, so the sealing effect is better, and the sealing ring can be replaced without replacing the entire sealing mechanism, thus improving the practicality of the device." As can be seen from the above, although the comparative patent has achieved the invention technical effects described to a certain extent, due to structural limitations, like other devices in this field, it still cannot take into account the advantages of the grating module and the filter module, and still has the problems existing in the above-mentioned prior art. To sum up, it is particularly necessary to provide a microplate reader that combines the advantages of the grating module and the filter module and can achieve better detection effects on corresponding samples. Summary of the Utility Model

[0005] In order to overcome the problem that the existing microplate readers cannot effectively take into account the advantages of the grating module and the filter module due to structural limitations, and the disadvantages that relevant departments need to equip two sets of microplate readers with grating modules and microplate readers with filter modules at the same time, which increases capital investment and is relatively inconvenient to operate, this utility model provides a new type of microplate reader based on the microplate reader body and accessories such as the grating module and the filter module. In application, under the joint action of relevant mechanisms, the tester can conveniently control the switching between the grating module and the filter module through the power switch to detect corresponding samples, and when the grating module and the filter module are accurately in place, it can timely prompt the tester to enter the detection process, which brings convenience to the staff, saves the equipment capital investment of relevant departments accordingly, and can achieve better detection effects on samples.

[0006] The technical solution adopted by this utility model to solve its technical problems is:

[0007] A new type of microplate reader based on a grating module and a filter module, comprising a microplate reader body, a grating module, a filter module, and a motor reduction mechanism. The control signal output end of the microprocessor system of the microplate reader body is electrically connected in parallel with the signal input ends of the grating module and the filter module. It is characterized in that it also has a detection mechanism, a first detection control circuit, and a second detection control circuit. The motor reduction mechanism is installed at the rear side end of the detection chamber of the microplate reader body. A support rod and a connecting rod are installed on the upper side end of the rotating shaft of the motor reduction mechanism. The rear ends of the grating module and the filter module are respectively installed at both ends of the front side of the connecting rod. The detection mechanism includes a variable resistor and a driven rubber wheel. The driven rubber wheel is installed on the adjustment handle of the variable resistor, and the variable resistor is installed on one side of the housing of the motor reduction mechanism. The first detection control circuit and the second detection control circuit are installed in the component chamber of the microplate reader body. The signal input ends of the first detection control circuit and the second detection control circuit are electrically connected to the signal output end of the variable resistor.

[0008] Further, when the grating module and the filter module are respectively rotated to the 90-degree position longitudinally in the detection chamber, they are respectively vertically located between the lower end of the light source lamp and the upper end of the photodetector of the microplate reader body.

[0009] Further, the side end of the driven rubber wheel rotates in contact with the side end of the rotating shaft of the motor reduction mechanism.

[0010] Further, the first detection control circuit includes a variable resistor, a resistor, a triode, and a light-emitting diode that are electrically connected. One end of the variable resistor is connected to one end of the first resistor and one end of the second resistor. The other end of the second resistor is connected to the base of the triode. The collector of the triode is connected to the negative electrode of the light-emitting diode. The positive electrode of the light-emitting diode is connected to one end of the third resistor. The other end of the first resistor is connected to the emitter of the triode.

[0011] Further, the second detection control circuit includes a variable resistor, a resistor, a triode, and a light-emitting diode that are electrically connected. One end of the variable resistor is connected to one end of the first resistor and one end of the second resistor. The other end of the second resistor is connected to the base of the first triode. The collector of the first triode is connected to one end of the third resistor and the base of the second triode. The other end of the first resistor is connected to the emitters of the two triodes. The other end of the third resistor is connected to one end of the fourth resistor. The other end of the fourth resistor is connected to the positive electrode of the light-emitting diode. The collector of the second triode is connected to the negative electrode of the light-emitting diode.

[0012] The beneficial effects of the present utility model are as follows: Based on the enzyme-labeling instrument body and accessories such as the grating module and the filter module, during application, under the combined action of relevant mechanisms, according to conditions such as the light transmittance of the detected sample and its different sensitivities to light, the tester can conveniently control the motor reduction mechanism through the power switch to drive the grating module and the filter module to be respectively located below the light source lamp in the detection chamber and above the photoelectric detector, so as to achieve the purpose of switching the grating module and the filter module to detect the corresponding sample. Moreover, after the grating module and the filter module are in place, it can promptly prompt the tester to enter the detection process. The present utility model brings convenience to the staff, correspondingly saves the equipment capital investment of relevant departments, and can achieve better detection effects on samples. Based on the above, the present utility model has good application prospects. Brief Description of the Drawings

[0013] The present utility model will be further described below in conjunction with the drawings and embodiments.

[0014] Figure 1 It is a schematic diagram of the overall structure and partial enlarged structure of the present utility model.

[0015] Figure 2 、 3 They are respectively schematic diagrams of the partial side view and top view plane structures of the present utility model.

[0016] Figure 4 It is the circuit diagram of the present utility model. Detailed Embodiment

[0017] Figure 1 、 2As shown in Figures 3 and 4, a new type of microplate reader based on a grating module and a filter module includes a power supply module A1, a microplate reader body 1, a grating module 2, a filter module 3, a motor reduction mechanism M, and a power switch S. The control signal output terminal of the microprocessor system (not shown in the figure) of the microplate reader body and the signal input terminals of the grating module 2 and the filter module 3 are connected in parallel via wires (with a length margin, and the wires are fixed to the side ends of the support rod 6 and the connecting rod 7); there is also a detection mechanism, a first detection control circuit 4, and a second detection control circuit 5; the motor reduction mechanism M is vertically installed in the middle of the rear side end of the detection chamber 101 of the microplate reader body. A support rod 6 is horizontally welded to the upper front side end of the rotating shaft of the motor reduction mechanism M. A semi-circular connecting rod 7 is welded to the front side end of the support rod 6. The middle parts of the rear outer side ends of the housings of the grating module 2 and the filter module 3 are respectively installed together with the front side end of the connecting rod 7 via bolts; the detection mechanism includes a variable resistor RP and a driven rubber wheel 8. The driven rubber wheel 8 is fixedly installed in the middle on the adjustment handle of the variable resistor RP. The lower end of the variable resistor RP is adhesively bonded to the upper right side of the housing of the motor reduction mechanism M; the power supply module A1, the power switch S (the handle is located at the front outer side end of the element addition chamber), the first detection control circuit 4, and the second detection control circuit 5 are installed in the element chamber of the microplate reader body 1.

[0018] Figure 1 、 2As shown in FIGS. 3 and 4, when the grating module 2 and the filter module 3 are respectively rotated to the longitudinal 90-degree position in the detection chamber 101, they are respectively vertically located between the lower end of the light source lamp 102 of the ELISA reader body and the upper end of the photoelectric detector 103. The left end of the driven rubber wheel 8 rotates in close contact with the right end of the rotating shaft of the motor reduction mechanism M. The first detection control circuit includes a variable resistor RP1, resistors R2, R3, R1, and transistors Q1 and an LED VL1 connected by circuit board wiring. One end of the variable resistor RP1 is connected to one end of the first resistor R1 and one end of the second resistor R2. The other end of the second resistor R2 is connected to the base of the transistor Q1. The collector of the transistor Q1 is connected to the negative electrode of the LED VL1. The positive electrode of the LED VL1 is connected to one end of the third resistor R3. The other end of the first resistor R1 is connected to the emitter of the transistor Q1. The second detection control circuit includes a variable resistor RP2, resistors R4, R5, R7, R6, and transistors Q2, Q3, and an LED VL2. One end of the variable resistor RP3 is connected to one end of the first resistor R4 and one end of the second resistor R5. The other end of the second resistor R5 is connected to the base of the first transistor Q2. The collector of the first transistor Q2 is connected to one end of the third resistor R6 and the base of the second transistor Q3. The other end of the first resistor R4 is connected to the emitters of the two transistors Q2 and Q3. The other end of the third resistor R6 is connected to one end of the fourth resistor R7. The other end of the fourth resistor R7 is connected to the positive electrode of the LED VL2. The collector of the second transistor Q3 is connected to the negative electrode of the LED VL2. The power input terminals 1 and 2 of the power module A1 and the two poles of the AC 220V power supply are respectively connected by wires. The power output terminals 3 and 4 of the power module A1, the power input terminals 1 and 2 of the power switch S, the other end of the resistor R3 at the power input terminal of the first detection control circuit and the emitter of the transistor Q1, and the other end of the resistor R7 at the power input terminal of the second detection control circuit and the emitter of the transistor Q2 are respectively connected by wires. The power output terminals 3 and 4 and 5 and 6 of the power switch S1 and the positive and negative and negative and positive power input terminals of the motor reduction mechanism M are respectively connected by wires. The signal input terminal of the first detection control circuit, the other end of the variable resistor RP1, the signal input terminal of the second detection control circuit, the other end of the variable resistor RP2, and the other end of the variable resistor RP are connected by wires. The other end of the variable resistor RP is connected to the positive power output terminal 3 of the power module A1 by a wire.

[0019] Figure 1 、 2As shown in Figures 3 and 4, during the application of the microplate reader main body 1, the inspector opens the door at the lower front end of the detection chamber, and then places the sample to be detected (located in a plastic microplate. After opening the detection chamber door, the plastic microplate 104 containing the test sample is placed on the detection station tray inside the detection chamber. After the detection is completed, the detection chamber door is opened, and the plastic microplate 104 containing the test sample is taken out) on the tray inside the detection chamber. After closing the door, the inspector controls the device to detect the sample by operating the buttons at the upper front end of the housing; the light wave emitted by the light source lamp 102 at the upper end inside the detection chamber of the microplate reader main body 1 passes through the grating module 2 or the filter module 3 and enters the specimen to be tested in the plastic microplate 104. Then, part of this monochromatic light is absorbed by the specimen, and the other part passes through the specimen and is irradiated onto the photodetector 103 at the lower end inside the microplate reader through the transparent tray. The photodetector 103 inputs the light signals with different intensities corresponding to different specimens to be tested into the signal input end of the conversion module inside the microplate reader main body 1. The conversion module converts it into corresponding electrical signals. After signal processing such as pre-amplification, logarithmic amplification, and analog-to-digital conversion, the electrical signals are sent to the microprocessor system of the microplate reader main body 1 for data processing and calculation. Finally, the data results of the detected sample (such as allergens, thyroid, inorganic toxins, etc.) are displayed on the display screen 105, or the data results are printed through an externally configured printer. The above is the existing mature technology, which will not be elaborated in this application, nor will any protection be provided for the above technical solutions. What this application protects is that a set of microplate reader main body 1 can conveniently switch between the grating module 2 and the filter module 3 to detect samples.

[0020] Figure 1 、 2As shown in Figures 3 and 4, after the AC 220V power supply enters the power input terminal of power module A1, the stable 12V DC power supply output from pins 3 and 4 of power module A1 enters the power input terminals of power switch S, the first detection and control circuit, and the second detection and control circuit. In this new type, after the tester toggles the handle of power switch S (the handle is outside the front end of the detection chamber) to the left or right, pins 1 and 2 and pins 3 and 4 or pins 5 and 6 of power switch S are respectively connected. In this way, the positive and negative or negative and positive power input terminals of the motor reduction mechanism M will be powered on. After the positive and negative power input terminals of the motor reduction mechanism M are powered on, its rotating shaft drives the grating module 2 and the filter module 3 to rotate clockwise. When the middle part of the filter module 3 is just located below the light source lamp 102, power switch S is turned off; after the negative and positive power input terminals of the motor reduction mechanism M are powered on, its rotating shaft drives the grating module 2 and the filter module 3 to rotate counterclockwise. When the middle part of the grating module 2 is just located below the light source lamp, power switch S is turned off. In actual situations, when the positive and negative power input terminals of the motor reduction mechanism M are powered on and its rotating shaft drives the grating module 2 and the filter module 3 to rotate clockwise, the rotating shaft will drive the driven rubber wheel 8 to rotate counterclockwise, and the driven rubber wheel 8 drives the handle of the adjustable resistor RP to rotate counterclockwise, and the resistance value of the adjustable resistor RP gradually becomes smaller; when the middle part of the filter module 3 is not located below the light source lamp 102, the rotating shaft drives the handle of the adjustable resistor RP to rotate counterclockwise through the driven rubber wheel 8 with a relatively small angle, and the resistance value of the adjustable resistor RP is relatively large. The 12V power supply is divided by the adjustable resistor RP, the adjustable resistor RP1, and the resistor R1, and the resistor R2 steps down the voltage and limits the current to enter the base of the triode Q1 below its 0.7V conduction voltage, and the triode Q1 will not conduct, the light-emitting diode VL1 will not be powered on and emit light, indicating that the middle part of the filter module 3 is not yet located below the light source lamp 102. When the middle part of the filter module 3 is located below the light source lamp 102, the rotating shaft drives the handle of the adjustable resistor RP to rotate counterclockwise through the driven rubber wheel 8 with a relatively large angle, and the resistance value of the adjustable resistor RP is relatively small. The 12V power supply is divided by the adjustable resistor RP, the adjustable resistor RP1, and the resistor R1, and the resistor R2 steps down the voltage and limits the current to enter the base of the triode Q1 above its 0.7V conduction voltage, and the triode Q1 will conduct and its collector outputs a low level to enter the negative power input terminal of the light-emitting diode VL1. The light-emitting diode VL1 is powered on and emits light (the resistor R3 steps down the voltage and limits the current), indicating that the middle part of the filter module 3 is located below the light source lamp 102 (at this moment, the tester immediately turns off the power switch, and the middle part of the filter module 3 is just located below the light source lamp 102, which facilitates the subsequent detection of the sample based on the filter module 3).When the middle part of the grating module 2 is not located below the light source lamp 102, the handle of the adjustable resistor RP is driven by the rotating shaft through the driven rubber wheel 8 to rotate clockwise by a relatively small angle, the resistance value of the adjustable resistor RP is relatively small, the 12V power supply is divided by the adjustable resistor RP, the adjustable resistor RP2 and the resistor R4, and the resistor R5 steps down and limits the current to enter the base of the triode Q2, which is higher than its 0.7V conduction voltage. The triode Q2 will conduct, and its collector outputs a low level to enter the base of the triode Q3. The base of the triode Q3 has no appropriate forward bias voltage and is cut off and will not conduct. The light-emitting diode VL2 is not powered on and does not emit light, indicating that the middle part of the grating module 2 has not yet been located below the light source lamp 102. When the middle part of the grating module 2 is located below the light source lamp 102, the handle of the adjustable resistor RP is driven by the rotating shaft through the driven rubber wheel 8 to rotate clockwise by a relatively large angle, the resistance value of the adjustable resistor RP is relatively large, the 12V power supply is divided by the adjustable resistor RP, the adjustable resistor RP2 and the resistor R4, and the resistor R2 steps down and limits the current to enter the base of the triode Q2, which is lower than its 0.7V conduction voltage. The triode Q2 will be cut off, and its collector no longer outputs a low level to enter the base of the triode Q3. The triode Q3 obtains an appropriate forward bias voltage through the resistor R6 to step down and limit the current from the positive pole of the 12V power supply and conducts. Its collector outputs a low level to enter the negative power input terminal of the light-emitting diode VL2 (the resistor R7 steps down and limits the current), and the light-emitting diode VL2 is powered on and emits light, indicating that the middle part of the grating module 2 is located below the light source lamp 102 (at this moment, the tester immediately turns off the power switch, and the middle part of the grating module 2 is just located below the light source lamp 102, which facilitates the subsequent detection of the sample based on the grating module 2).

[0021] Figure 1 , 2 As shown in Figures 3 and 4 above, through the above, the present invention is based on the enzyme-labeling instrument body and accessories such as the grating module and the filter module. In application, under the joint action of relevant mechanisms, according to conditions such as the light transmittance of the detected sample and its sensitivity to light, the tester can conveniently control the motor reduction mechanism through the power switch to drive the grating module and the filter module to be respectively located below the light source lamp in the detection chamber and above the photodetector, so as to achieve the purpose of switching the grating module and the filter module to detect the corresponding sample. And after the grating module and the filter module are in place, they can promptly indicate to the tester to enter the detection process (the motor reduction mechanism M rotates at a low speed, and the rotating shaft can stop immediately after a power outage). The present invention brings convenience to the staff, correspondingly saves the equipment capital investment of relevant departments, and can achieve better detection effects on samples. Figure 4Among them, the power supply module A1 is a finished product of an AC 220V to DC 12V switching power supply module; the motor reduction mechanism M is a finished product of a coaxial motor gear reducer with a power of 15W, and its rotating shaft rotates at 6 revolutions per minute; the light-emitting diodes VL1 and VL2 are red and green light-emitting diodes respectively (the light-emitting surfaces are respectively located outside two openings at the front end of the housing of the microplate reader body); the triodes Q1, Q2, and Q3 are NPN-type triodes of model 9013; the resistance values of the resistors R1, R2, R3, R4, R5, R6, and R7 are 10K, 4.7K, 1.8K, 10K, 4.7K, 100K, and 1.8K respectively; the resistance values of the adjustable resistors RP, RP1, and RP2 are 470K (the resistance values of the adjustable resistors RP1 and RP2 are adjusted to 92K and 84K respectively; when the resistance values of the adjustable resistors RP1 and RP2 are adjusted larger, the voltage division with the resistors R1 and R4 is larger respectively. Then, when the resistance value of the adjustable resistor RP is relatively small, that is, when the handle of the adjustable resistor RP rotates counterclockwise by a larger angle or clockwise by a smaller angle, the triode Q1 or Q2 will conduct, that is, the rotation angles of the grating module and the filter module are set relatively small; when the resistance values of the adjustable resistors RP1 and RP2 are adjusted smaller, the voltage division with the resistors R1 and R4 is smaller respectively. Then, when the resistance value of the adjustable resistor RP is relatively large, that is, when the handle of the adjustable resistor RP rotates counterclockwise by a smaller angle or clockwise by a larger angle, the triode Q1 or Q2 will conduct, that is, the rotation angles of the grating module and the filter module are set relatively large; during actual production, the staff adjusts the resistances of the adjustable resistors RP1 and RP2 respectively. When the middle parts of the grating module and the filter module are just located below the light source lamp, the resistance values are adjusted to the required resistance values). The components used in this application are all existing and mature industrial products, and this application will not elaborate on the working principles of each component.

[0022] The above shows and describes the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited by the above embodiments. What is described in the above embodiments and the specification only illustrates the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention will have various changes and improvements, and these changes and improvements all fall within the scope of the present invention claimed. The scope of protection claimed by the present invention is defined by the appended claims and their equivalents.

Claims

1. A novel microplate reader based on a grating module and a filter module, comprising an electric microplate reader body, a grating module, a filter module, and a motor deceleration mechanism, wherein a control signal output end of a microprocessor system of the microplate reader body and a signal input end of the grating module and the filter module are electrically connected in parallel; characterized in that: It also has a detection mechanism, a first detection control circuit, and a second detection control circuit; the motor reduction mechanism is installed at the rear end of the detection compartment of the ELISA instrument body, and the upper end of the rotating shaft of the motor reduction mechanism is installed with a support rod and a connecting rod, and the rear ends of the grating module and the filter module are respectively installed together with the two ends of the front side of the connecting rod; the detection mechanism includes an adjustable resistor and a driven rubber wheel, the driven rubber wheel is installed on the adjustment handle of the adjustable resistor, and the adjustable resistor is installed on one side of the shell of the motor reduction mechanism; the first detection control circuit and the second detection control circuit are installed in the component compartment of the ELISA instrument body, and the signal input ends of the first detection control circuit and the second detection control circuit are electrically connected to the signal output ends of the adjustable resistor.

2. The novel microplate reader based on the grating module and the filter module according to claim 1 is characterized in that: When the grating module and the filter module are rotated to the longitudinal position of 90 degrees in the detection chamber, they are respectively located vertically between the lower end of the light source lamp of the ELISA instrument body and the upper end of the photoelectric detector.

3. The novel microplate reader based on the grating module and the filter module according to claim 1 is characterized in that: The side end of the driven rubber wheel rotates to contact the side end of the rotating shaft of the motor reduction mechanism.

4. The novel microplate reader based on the grating module and the filter module according to claim 1 is characterized in that: The first detection control circuit includes an electrically connected adjustable resistor, a resistor and a transistor, and a light-emitting diode. One end of the adjustable resistor is connected to one end of the first resistor and one end of the second resistor. The other end of the second resistor is connected to the base of the transistor. The collector of the transistor is connected to the cathode of the light-emitting diode. The anode of the light-emitting diode is connected to one end of the third resistor. The other end of the first resistor is connected to the emitter of the transistor.

5. The novel microplate reader based on the grating module and the filter module according to claim 1 is characterized in that: The second detection control circuit includes an electrically connected adjustable resistor, a resistor and a transistor, and a light-emitting diode. One end of the adjustable resistor is connected to one end of the first resistor and one end of the second resistor, the other end of the second resistor is connected to the base of the first transistor, the collector of the first transistor is connected to one end of the third resistor and the base of the second transistor, the other end of the first resistor is connected to the emitters of the two transistors, the other end of the third resistor is connected to one end of the fourth resistor, the other end of the fourth resistor is connected to the positive electrode of the light-emitting diode, and the collector of the second transistor is connected to the negative electrode of the light-emitting diode.

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