Determination device for inhibitory concentration of pathogenic drug

Abstract

The utility model discloses a pathogenic drug inhibition concentration measuring device which comprises a container shell and partition plate assemblies, a plurality of positioning grooves are formed in the inner wall of the container shell at intervals, and the partition plate assemblies are inserted into the positioning grooves respectively so as to divide the container shell into a plurality of independent small chambers. And the solutions in different small chambers are mixed by inserting and pulling different partition plate assemblies, so that the concentrations of the solutions are diluted in a gradient manner. The pathogenic drug inhibition concentration determination device is simple to operate, and can rapidly dilute the concentration of the drug liquid in a gradient manner.

Description

Technical Field

[0001] The utility model belongs to the technical field of drug testing, and particularly relates to a device for measuring the inhibitory concentration of pathogenic drugs. Background Art

[0002] The minimum inhibitory concentration (MIC) refers to the lowest drug concentration that can inhibit the growth of pathogenic bacteria in a culture medium after culturing bacteria in vitro for 18 to 24 hours, and it is an index for measuring the antibacterial activity of antibacterial drugs. The minimum bactericidal concentration (MBC) refers to the lowest drug concentration required to kill 99.9%

[0003] (reduce by three orders of magnitude) of the test microorganisms. MIC and MBC are important indicators of the resistance of antibacterial drugs to bacteria in diagnostic laboratories. At the same time, MIC and MBC are also very important bases for verifying the effects of new antibacterial drugs. The lower the MIC and MBC, the better the effect on bacteria. MIC and MBC are generally regarded as the most basic laboratory measurement indicators for the efficacy of antibacterial agents.

[0004] In the prior art, in the process of measuring MIC and MBC in a laboratory, a variety of tools such as 96-well plates, micropipettes, conical flasks, test tubes, etc. are often used in combination. There are often operations such as preparing different concentrations of liquid medicines, adding the liquid medicines to the culture solutions respectively, and adding bacterial solutions to the culture solutions respectively. However, ordinary pipettes and micropipettes not only have cumbersome operations and low efficiency, but also are prone to contamination due to the repeated use of tools, which affects the experimental results.

[0005] Based on this, it is necessary to provide a device for quickly measuring the MIC value and MBC value of bacteria. Summary of the Utility Model

[0006] The technical problem to be solved by the utility model is to provide a device for measuring the inhibitory concentration of pathogenic drugs, aiming at the above deficiencies in the prior art. The device for measuring the inhibitory concentration of pathogenic drugs has simple operation and can quickly gradient-dilute the concentration of the liquid medicine.

[0007] To solve the above problems, the utility model adopts the following technical scheme:

[0008] A device for measuring the inhibitory concentration of pathogenic drugs includes a container housing and a partition assembly. A plurality of positioning grooves are provided at intervals on the inner wall of the container housing, and a plurality of partition assemblies are respectively inserted into the positioning grooves to divide the container housing into a plurality of separate small chambers, and the solutions in different small chambers are mixed by inserting and removing different partition assemblies, so as to gradient-dilute the concentration of the solution.

[0009] Preferably, the partition assembly includes a partition and a top plate. The top plate is fixedly installed on the top of the partition and is perpendicular to the partition. When the partition is inserted into the positioning groove, both ends of the top plate contact the inner side wall of the container housing.

[0010] Preferably, the partition assembly further includes a control handle, and the control handle is fixedly installed on the top of the top plate.

[0011] Preferably, the container housing is provided with a first scale along its length direction, a second scale along its height direction, and a third scale along its width direction.

[0012] Preferably, the container housing is made of a translucent material.

[0013] Preferably, the material of the container housing is acrylic or glass.

[0014] Preferably, the middle part of the partition is made of PVC material, and the edge is made of silicone material.

[0015] Preferably, the pathogenic drug inhibitory concentration measuring device further includes a temperature monitoring component. The temperature monitoring component includes an electronic thermometer and a temperature display module. The electronic thermometer is arranged inside the container housing for monitoring the temperature of the solution, and the temperature display module is electrically connected to the electronic thermometer for real-time displaying the temperature of the solution inside the container housing.

[0016] Preferably, the pathogenic drug inhibitory concentration measuring device further includes a top cover. The top of the container housing is open, and the top cover closes the open part of the container housing.

[0017] The pathogenic drug inhibitory concentration measuring device in the present utility model solves the problems of cumbersome dilution of the medicinal liquid and addition of bacterial liquid in traditional experiments, can rapidly perform gradient dilution of the medicinal liquid concentration, and simultaneously omits the step of gradually adding bacterial liquid, thereby rapidly measuring the MIC and MBC values of bacteria. And the device has a simple principle, strong portability, and is easy to operate. Description of the Drawings

[0018] Figure 1 is a schematic structural diagram of the pathogenic drug inhibitory concentration measuring device in Embodiment 1 of the present utility model with a partition installed;

[0019] Figure 2 is a schematic structural diagram of the pathogenic drug inhibitory concentration measuring device in Embodiment 1 of the present utility model without a partition installed;

[0020] Figure 3 is a front view of the pathogenic drug inhibitory concentration measuring device in Embodiment 1 of the present utility model;

[0021] Figure 4 It is the top view of the pathogenic drug inhibitory concentration measuring device in Embodiment 1 of the present utility model;

[0022] Figure 5 It is the structural schematic diagram of the partition assembly in Embodiment 1 of the present utility model.

[0023] In the figure: 100 - container outer shell, 110 - positioning groove, 200 - partition assembly, 210 - partition, 220 - top plate, 230 - control handle. Specific embodiments

[0024] Next, the technical solutions in the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the present utility model. Obviously, the described embodiments are part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments in the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the scope of the present utility model.

[0025] In the description of the present utility model, it should be noted that the terms such as "upper" indicating the orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of description and simplification, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus cannot be understood as a limitation to the present utility model.

[0026] In the description of the present utility model, the terms "first" and "second" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance.

[0027] In the description of the present utility model, it should be noted that unless otherwise clearly defined and limited, the terms "connection", "arrangement", "installation", "fixation", etc. should be understood in a broad sense. For example, it can be a fixed connection or a detachable connection, or an integral connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the internal communication of two elements. For those skilled in the art, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.

[0028] The present utility model provides a pathogenic drug inhibitory concentration measuring device, including a container outer shell and a partition assembly. A plurality of positioning grooves are arranged at intervals on the inner wall of the container outer shell, and a plurality of partition assemblies are respectively inserted into the positioning grooves to divide the container outer shell into a plurality of separate small chambers, and the solutions in different small chambers are mixed by inserting and removing different partition assemblies, so as to perform gradient dilution of the solution concentration.

[0029] Embodiment 1

[0030] As Figure 1 、2 As shown in the figure, this embodiment discloses a device for measuring the inhibitory concentration of pathogenic drugs, which includes a container housing 100 and a partition assembly 200. A plurality of vertical positioning grooves 110 are spaced apart on the inner wall of the container housing 100, and a plurality of partition assemblies 200 are respectively inserted into the positioning grooves 110 to divide the container housing 100 into a plurality of small chambers with the same volume. By inserting and removing different partition assemblies 200, the solutions in different small chambers are mixed, thereby performing gradient dilution of the solution concentration.

[0031] Specifically, the device for measuring the inhibitory concentration of pathogenic drugs in this embodiment is used to measure the minimum inhibitory concentration and the minimum bactericidal concentration of bacteria. During measurement, a quantitative culture medium needs to be added to the inside of the container housing 100, and then a plurality of partition assemblies 200 are respectively inserted into a plurality of positioning grooves 110 to divide the container housing 100 into a plurality of small chambers with the same volume. Then, an appropriate amount of bacterial liquid and the test drug solution are added to the first small chamber. After waiting for the mixture to be uniform, the partition assemblies 200 of the first small chamber and the second small chamber are opened so that the solutions in the two small chambers are mixed evenly, thereby diluting the drug solution concentration in the first small chamber by 1 / 2 times. By analogy, when the plurality of partition assemblies 200 are opened in sequence, the drug solution concentration in the first small chamber can be diluted by (1 / 2) n times.

[0032] As Figure 5 shown in the figure, in this embodiment, the partition assembly 200 includes a partition 210 and a top plate 220. The top plate 220 is fixedly installed on the top of the partition 210 and is perpendicular to the partition 210. The partition 210 is used to be inserted into the positioning groove 110 to separate different small chambers. When the partition 210 is inserted into the positioning groove 110, both ends of the top plate 220 are in contact with the inner side wall of the container housing 100 to ensure that the partition 210 can be inserted into the positioning groove 110 in a vertical state.

[0033] As Figure 1 shown in the figure, specifically, the partition assembly 200 further includes a control handle 230. The control handle 230 is fixedly installed on the top of the top plate 220, and the operator inserts and removes the partition 210 by holding the control handle 230.

[0034] The container housing 100 is provided with a first scale along its length direction, a second scale along its height direction, and a third scale along its width direction. By reading the first scale, the second scale, and the third scale, the three-dimensional dimensions of a single small chamber can be quickly obtained, and the volume of the small chamber can be calculated through calculation. Therefore, when adding bacterial liquid and drug solution to the small chamber, the concentrations of the bacterial liquid and the drug solution can be calculated based on the volume of the small chamber.

[0035] For easy observation, the container housing 100 is made of a translucent material. Specifically, the container housing 100 can be made of acrylic or glass material to clearly observe the height of the liquid level and the situation inside the solution.

[0036] In this embodiment, the middle part of the partition 210 is made of hard PVC material, and the edge is made of silicone material. When the partition 210 is inserted into the positioning groove 110, the silicone at its edge is in close contact with the positioning groove 110, thus ensuring the sealing performance between two adjacent small chambers.

[0037] Optionally, the pathogenic drug inhibition concentration measuring device further includes a temperature monitoring component. The temperature monitoring component includes an electronic thermometer and a temperature display module. The electronic thermometer is arranged inside the container housing 100 and is in contact with the internal solution to monitor the temperature of the solution. The temperature display module is electrically connected to the electronic thermometer to display the temperature of the solution inside the container housing 100 in real time. Through the temperature monitoring component, the temperature of the solution inside the container housing 100 can be monitored in real time to ensure that the liquid medicine and the bacterial liquid are within the temperature range specified in the experiment.

[0038] Optionally, the pathogenic drug inhibition concentration measuring device further includes a top cover. The container housing 100 is in the shape of a cuboid with an open top, and the top cover closes the open top of the container housing 100. During the experiment, the top cover can be covered on the top of the container housing 100 to prevent bacteria in the air from contaminating the solution inside the container housing 100.

[0039] As Figure 3 shown, specifically, the container housing 100 is provided with a total of seventeen positioning grooves 110, and the distance between every two adjacent positioning grooves 110 is the same. Therefore, at most seventeen partitions 210 can be set, dividing the container housing 100 into eighteen small chambers.

[0040] As Figure 1 、 4 shown, in this embodiment, a total of five partitions 210 are set, thus dividing the container housing 100 into six small chambers with the same volume, and two adjacent partitions 210 are spaced three positioning grooves 110 apart.

[0041] The using process of the pathogenic drug inhibition concentration measuring device in this embodiment is as follows:

[0042] First, pour a quantitative culture medium into the container housing 100 without installing the partition 210;

[0043] The operator inserts n partitions 210 into n positioning grooves 110 of the container housing 100, dividing the container housing 100 into n + 1 small chambers with equal volume. The partition 210 is tightly connected to the positioning slot, and the silicone edge of the partition 210 can isolate the material exchange between two adjacent small chambers;

[0044] Add appropriate amount of bacterial solution and drug solution to be tested to the first chamber, let it stand for a while and wait for it to be evenly mixed;

[0045] Then, the partition 210 between the first chamber and the second chamber is pulled out to allow the liquids in the two chambers to mix evenly, and then the partition 210 between the first chamber and the second chamber is inserted back to complete the first dilution. At this time, the concentrations of the liquids in the first chamber and the second chamber are both 1 / 2 times of the initial concentration.

[0046] During the second dilution, the partition 210 between the second chamber and the third chamber is pulled out to make the liquid in the second chamber and the third chamber evenly mixed, and the concentration of the liquid in the second chamber and the third chamber is 1 / 2 times that of the first dilution, and then the partition 210 between the second chamber and the third chamber is inserted back;

[0047] By analogy, all the partitions 210 are pulled out in sequence. When the number of partitions 210 is n, the concentration of the diluted drug solution is (1 / 2) of the initial concentration. n ;

[0048] If a control group is required for the bacterial experiment, a small chamber can be reserved at both ends of the container shell 100. During the dilution process, the partition 210 between it and other small chambers is not pulled out, and the small chamber is the control group without adding bacterial solution or drug solution;

[0049] If the volume ratio of the chambers on both sides of each spacer is adjusted (ie, the volume of each chamber is different), gradient dilutions of other multiples can be achieved.

[0050] The pathogenic drug inhibition concentration determination device in this embodiment solves the cumbersome and complicated problems of diluting the drug solution and adding the bacterial solution in the traditional experiment, and can quickly dilute the drug solution concentration in a gradient manner, while eliminating the step of gradually adding the bacterial solution, thereby quickly measuring the MIC and MBC values ​​of the bacteria. In addition, the device has a simple principle, strong portability, and is easy to operate.

[0051] It is understood that the above embodiments are merely exemplary embodiments used to illustrate the principles of the present invention, but the present invention is not limited thereto. For those skilled in the art, various modifications and improvements can be made without departing from the spirit and essence of the present invention, and these modifications and improvements are also considered to be within the scope of protection of the present invention.

Claims

1. A pathogenic drug inhibition concentration measuring device, characterized in that: It comprises a container shell (100), a partition assembly (200), A plurality of positioning grooves (110) are arranged at intervals on the inner wall of the container shell (100), and a plurality of partition assemblies (200) are respectively inserted into the positioning grooves (110) to separate the container shell (100) into a plurality of separate small chambers. By plugging and unplugging different partition assemblies (200), solutions in different small chambers are mixed, thereby gradient diluting the concentration of the solution.

2. The pathogenic drug inhibition concentration measuring device according to claim 1, characterized in that: The partition assembly (200) comprises a partition (210) and a top plate (220). The top plate (220) is fixedly mounted on the top of the partition plate (210) and is perpendicular to the partition plate (210). When the partition plate (210) is inserted into the positioning groove (110), both ends of the top plate (220) are in contact with the inner side wall of the container shell (100).

3. The pathogenic drug inhibition concentration measuring device according to claim 2, characterized in that: The partition assembly (200) further includes a control handle (230), The control handle (230) is fixedly mounted on the top of the top plate (220).

4. The pathogenic drug inhibition concentration measuring device according to claim 1, characterized in that: The container shell (100) is provided with a first scale along its length direction, a second scale along its height direction, and a third scale along its width direction.

5. The pathogenic drug inhibition concentration measuring device according to claim 1, characterized in that: The container shell (100) is made of translucent material.

6. The pathogenic drug inhibition concentration measuring device according to claim 5, characterized in that: The container shell (100) is made of acrylic or glass.

7. The pathogenic drug inhibition concentration measuring device according to claim 2, characterized in that: The middle part of the partition (210) is made of PVC material, and the edge is made of silica gel material.

8. The pathogenic drug inhibition concentration measuring device according to claim 1, characterized in that: It also includes a temperature monitoring component, which includes an electronic thermometer and a temperature display module. The electronic thermometer is arranged inside the container shell (100) and is used to monitor the temperature of the solution. The temperature display module is electrically connected to the electronic thermometer and is used to display the temperature of the solution in the container shell (100) in real time.

9. The pathogenic drug inhibition concentration measuring device according to any one of claims 1 to 8, characterized in that: Also includes the top cover, The top of the container shell (100) is open, and the top cover closes the open opening of the container shell (100).

Images