Efficient incubator for plant tissue culture

Abstract

The utility model relates to the technical field of plant tissue culture, and discloses an efficient incubator for plant tissue culture, which comprises an incubator, a push handle and four universal wheels, a door is hinged to the front of the incubator, an observation window is arranged on the front of the door in a penetrating manner, and the inner wall of the observation window is fixedly connected with the observation window. A culture mechanism and a ventilation mechanism are arranged in the culture box, and the ventilation mechanism is located above the culture mechanism. Through the culture mechanism and the fixing assembly, culture flasks are fixedly clamped on a culture plate through clamping plates and springs, the culture flasks are conveniently and intensively placed in a culture box, and the rotating assembly drives the culture plate to rotate through rotation of a first motor, so that rotation of the culture flasks is achieved, and the plant tissue culture condition in the culture flasks is conveniently observed; observation can be carried out without taking the culture bottle out of the culture box, meanwhile, a culture solution can be conveniently added into the culture bottle, and the convenience and high efficiency of the culture box on plant tissue culture observation are improved.

Description

Technical Field

[0001] The utility model relates to the technical field of plant tissue culture, and specifically relates to an efficient incubator for plant tissue culture. Background Technique

[0002] The tissue culture of plants is also called in vitro culture in a broad sense, which refers to separating tissues, organs or cells, protoplasts, etc. that meet the requirements from the plant body, and through aseptic operation, inoculating them on a culture medium containing various nutrients and plant hormones under aseptic conditions for cultivation to obtain a regenerated complete plant or produce other products with economic value. Narrowly speaking, tissue culture refers to culturing various parts of plants, such as cambium, parenchyma, mesophyll tissue, endosperm, etc. to obtain regenerated plants, and also refers to the culture of callus generated from various organs during the culture process. The callus is then redifferentiated to form regenerated plants. When culturing plant tissues, an incubator is required. The incubator can effectively culture plant tissues and is a special culture device.

[0003] Chinese patent document CN213044631U discloses an efficient incubator for plant tissue culture, including a box door hinged on the front surface of the incubator, and a lighting lamp fixedly connected to the upper surface inside the incubator. A liquid inlet pipe is installed through the upper surface of the incubator. A recycling component is arranged on the lower surface inside the incubator, and an adjusting component is fixedly installed on the lower surface inside the incubator. The upper end of the adjusting component is fixedly connected to a culture plate. A diversion rope is arranged on the lower surface of the culture plate. By setting the adjusting component, the height of the culture plate arranged at the upper ends of the first telescopic column and the second telescopic column can be effectively adjusted, which is beneficial to adjusting the light intensity of the lighting lamp received by the plants and improving photosynthesis. By setting the recycling component, the excess nutrient solution sprayed can be effectively recycled and utilized, avoiding waste and preventing pollution on the surface of the culture plate.

[0004] The culture plate in this device is fixed at the upper end of the adjusting component, and the height of the culture plate is adjusted by adjusting the first telescopic column and the second telescopic column to realize the adjustment of the light intensity of the lighting lamp received by the plants. However, when culturing plant tissues, the culture bottle needs to be placed on the culture plate for cultivation. When it is necessary to observe the cultivation situation of the plant tissues inside the culture bottle, it can only be observed by taking out the culture bottle, which is not convenient for observing the plant tissue culture. Content of the Utility Model

[0005] The purpose of the utility model is to provide an efficient incubator for plant tissue culture to solve the problems raised in the above background technique.

[0006] To achieve the above object, the present utility model provides the following technical solution: An efficient incubator for plant tissue culture, comprising an incubator, a push handle and four universal wheels. The front of the incubator is hinged with a door, and an observation window is formed through the front of the door. An observation window is fixedly connected to the inner wall of the observation window. A culture mechanism and a ventilation mechanism are arranged inside the incubator, and the ventilation mechanism is located above the culture mechanism; the culture mechanism includes a fixing component and a rotating component, and the fixing component is located above the rotating component;

[0007] The rotating component includes a first motor, the bottom surface of the first motor is fixedly connected to the inner bottom surface of the incubator, and a connecting block and a fixing block are arranged above the first motor;

[0008] The fixing component includes a culture plate, a ring plate is fixedly connected to the top surface of the culture plate, two clamping plates are arranged inside the ring plate, the two clamping plates are symmetrically arranged, a protection plate is arranged inside the clamping plates, a culture bottle is arranged between the two clamping plates, a pull rod is arranged outside the clamping plates, and a spring is sleeved on the surface of the pull rod.

[0009] Preferably, the top surface of the output rod of the first motor is fixedly connected to the bottom surface of the connecting block, a chute adapted to the connecting block is formed through the bottom surface of the fixing block, and the top surface of the connecting block slides through the bottom surface of the fixing block and extends into the chute.

[0010] Preferably, the inner wall of the fixing block is threadedly connected to the inner wall of the connecting block by bolts, and the top surface of the fixing block is fixedly connected to the bottom surface of the culture plate. The culture plate is connected by the fixing block and the connecting block, so that the culture plate can rotate.

[0011] Preferably, the inner side surface of the clamping plate is fixedly connected to the outer side surface of the protection plate, the inner side surface of the protection plate is in pressing connection with the surface of the culture bottle, and the protection plate is made of rubber material. The protection plate can prevent the clamping plate from damaging the surface of the culture bottle.

[0012] Preferably, the outer side surface of the clamping plate is fixedly connected to the inner end surface of the pull rod, the outer end surface of the pull rod slides through the inner side surface of the ring plate and extends to the outside of the ring plate, the inner end surface of the spring is fixedly connected to the outer side surface of the clamping plate, and the outer end surface of the spring is fixedly connected to the inner side surface of the ring plate. The clamping plate can be moved conveniently through the pull rod.

[0013] Preferably, the right side surface of the push handle is fixedly connected to the left side surface of the incubator, and the top surfaces of the four universal wheels are all fixedly connected to the bottom surface of the incubator. The universal wheels facilitate the movement of the incubator.

[0014] Preferably, the ventilation mechanism includes a ventilation frame. The bottom end surface of the ventilation frame fixedly penetrates through the top surface of the incubator and extends into the interior of the incubator. A second motor is fixedly connected to the inner top surface of the ventilation frame. The bottom end surface of the output rod of the second motor is fixedly connected to a fan. A ventilation opening is formed through the top surface of the ventilation frame. A first filter screen is arranged above the ventilation opening. An air outlet is formed through the bottom surface of the incubator. A second filter screen is fixedly connected to the inner wall of the air outlet. The first filter screen is made of a metal material filter screen, which is convenient for ventilating the incubator.

[0015] Preferably, a placement groove is formed through the top surface of the ventilation frame. A magnetic strip is fixedly connected to the inner wall of the placement groove. The top surface of the magnetic strip is magnetically connected to the bottom surface of the first filter screen. The magnetic strip facilitates the installation and disassembly of the first filter screen, and it is convenient to disassemble the first filter screen for cleaning.

[0016] Compared with the prior art, the beneficial effects of the high-efficiency incubator for plant tissue culture of the present invention are as follows: 1. Through the culture mechanism, the fixing component uses the clamping plate and the spring to fixedly clamp the culture bottle on the culture plate, which is convenient for the culture bottles to be centrally placed in the incubator. The rotating component uses the first motor to drive the culture plate to rotate, so as to realize the rotation of the culture bottle, which is convenient for observing the culture situation of the plant tissue in the culture bottle. It is not necessary to take out the culture bottle from the incubator for observation, and at the same time, it is convenient to add culture solution to the culture bottle, improving the convenience and efficiency of the incubator for observing the plant tissue culture; 2. Through the ventilation mechanism, the second motor drives the fan to rotate to ventilate the incubator, which can avoid the normal air circulation speed in the incubator when there is no wind outside the incubator, and improve the growth environment of the plant tissue culture in the incubator. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 is the front sectional perspective view of the present invention;

[0018] Figure 2 is the overall front view perspective view of the present invention;

[0019] Figure 3 is the overall bottom view perspective view of the present invention;

[0020] Figure 4 is the front view perspective view of the clamping plate in the present invention;

[0021] Figure 5 is the bottom view perspective view of the fixing block in the present invention;

[0022] Figure 6 is Figure 1 the partial enlarged perspective view of part A in

[0023] In the figure: incubator 1, universal wheels 2, box door 3, push handle 4, observation window 5, culture mechanism 6, culture plate 601, ring plate 602, clamping plate 603, protection plate 604, pull rod 605, spring 606, fixed block 607, first motor 608, connecting block 609, culture bottle 610, ventilation mechanism 7, ventilation frame 701, second motor 702, fan 703, first filter screen 704, magnetic strip 705, second filter screen 706. Detailed implementation

[0024] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention. Embodiment 1

[0025] Please refer to Figure 1 - Figure 6 , the high-efficiency incubator for plant tissue culture described in this embodiment includes an incubator 1, a push handle 4 and four universal wheels 2. A box door 3 is hinged to the front of the incubator 1. An observation window is formed through the front of the box door 3. An observation window 5 is fixedly connected to the inner wall of the observation window. A culture mechanism 6 and a ventilation mechanism 7 are arranged inside the incubator 1. The ventilation mechanism 7 is located above the culture mechanism 6. A lighting lamp (not shown in the figure) is arranged inside the incubator 1.

[0026] The culture mechanism 6 includes a fixing component and a rotating component. The fixing component is located above the rotating component.

[0027] The rotating component includes a first motor 608. The bottom surface of the first motor 608 is fixedly connected to the inner bottom surface of the incubator 1. A connecting block 609 and a fixed block 607 are arranged above the first motor 608.

[0028] The fixing component includes a culture plate 601. A ring plate 602 is fixedly connected to the top surface of the culture plate 601. Two clamping plates 603 are arranged inside the ring plate 602. The two clamping plates 603 are symmetrically arranged. A protection plate 604 is arranged on the inner side of the clamping plates 603. A culture bottle 610 is arranged between the two clamping plates 603. A pull rod 605 is arranged on the outer side of the clamping plates 603. A spring 606 is sleeved on the surface of the pull rod 605. The top end surface of the output rod of the first motor 608 is fixedly connected to the bottom surface of the connecting block 609. A chute adapted to the connecting block 609 is formed through the bottom surface of the fixed block 607. The top end surface of the connecting block 609 slidably penetrates through the bottom surface of the fixed block 607 and extends into the inside of the chute. The inner wall of the fixed block 607 is threadedly connected to the inner wall of the connecting block 609 through a bolt. The top surface of the fixed block 607 is fixedly connected to the bottom surface of the culture plate 601. The inner side surface of the clamping plate 603 is fixedly connected to the outer side surface of the protection plate 604. The inner side surface of the protection plate 604 is in pressing connection with the surface of the culture bottle 610. The protection plate 604 is made of rubber material. The outer side surface of the clamping plate 603 is fixedly connected to the inner end surface of the pull rod 605. The outer end surface of the pull rod 605 slidably penetrates through the inner side surface of the ring plate 602 and extends to the outside of the ring plate 602. The inner end surface of the spring 606 is fixedly connected to the outer side surface of the clamping plate 603. The outer end surface of the spring 606 is fixedly connected to the inner side surface of the ring plate 602. Through the culture mechanism 6, the fixing component uses the clamping plates 603 and the spring 606 to fixedly clamp the culture bottle 610 on the culture plate 601, facilitating the centralized placement of the culture bottle 610 into the incubator 1. The rotation component drives the rotation of the culture plate 601 by the rotation of the first motor 608, thereby realizing the rotation of the culture bottle 601, facilitating the observation of the plant tissue culture situation in the culture bottle 610. Observation can be carried out without taking the culture bottle 610 out of the incubator 1. At the same time, it is convenient to add culture solution to the culture bottle 610, improving the convenience and efficiency of the incubator for observing plant tissue culture.

[0029] The right side surface of the push handle 4 is fixedly connected to the left side surface of the incubator 1. The top surfaces of the four universal wheels 2 are all fixedly connected to the bottom surface of the incubator 1. Embodiment 2

[0030] As Figure 1 、 Figure 2 and Figure 5As shown, on the basis of Embodiment 1, in the high-efficiency incubator for plant tissue culture described in this embodiment, the ventilation mechanism 7 includes a ventilation frame 701. The bottom end surface of the ventilation frame 701 fixedly penetrates through the top surface of the incubator 1 and extends into the interior of the incubator 1. A second motor 702 is fixedly connected to the inner top surface of the ventilation frame 701. The bottom end surface of the output rod of the second motor 702 is fixedly connected to a fan 703. A ventilation opening is formed through the top surface of the ventilation frame 701. A first filter screen 704 is arranged above the ventilation opening. An air outlet is formed through the bottom surface of the incubator 1. A second filter screen 706 is fixedly connected to the inner wall of the air outlet. The first filter screen 704 is made of a metal material filter screen. A placement groove is formed through the top surface of the ventilation frame 701. A magnetic strip 705 is fixedly connected to the inner wall of the placement groove. The top surface of the magnetic strip 705 is magnetically connected to the bottom surface of the first filter screen 704. Through the ventilation mechanism 7, the second motor 702 drives the fan 703 to rotate to ventilate the incubator 1, which can avoid the normal air circulation speed inside the incubator 1 when there is no wind outside the incubator 1, and improve the growth environment of the incubator for plant tissue culture.

[0031] During use, pull the pull rod 605 outward. The pull rod 605 drives the connected clamping plate 603 to move outward. The clamping plate 603 squeezes the connected spring 606. Place the culture bottle 610 between the two clamping plates 603. Release the pull rod 605. The spring 606 forces the connected clamping plate 603 to move. The clamping plate 603 drives the connected protection plate 604 to move towards the culture bottle 610 and clamps and fixes the culture bottle 610 on the culture plate 601. Open the box door 3. Place the culture plate 601 into the incubator 1. The connecting block 609 slides into the chute. Use bolts to fix the fixing block 607 and the connecting block 609, thereby fixing the culture plate 601 in the incubator 1. Turn on the second motor 702. The output rod of the second motor 702 drives the connected fan 703 to rotate, so that the fan 703 increases the air circulation inside the ventilation frame 701. The flowing air enters the incubator 1, thereby realizing ventilation of the culture bottle 610 in the incubator 1. When it is necessary to observe the plant tissue culture situation in the culture bottle 610, turn on the first motor 608. The output rod of the first motor 608 drives the connected connecting block 609 to rotate, so that the culture plate 601 rotates and drives the culture bottle 610 to rotate. The plant tissue culture situation inside the culture bottle 610 can be observed through the observation window 5. At the same time, the box door 3 can be opened to add nutrient solution to the culture bottle 610, and the culture plate 601 drives the culture bottle 610 to rotate to add nutrient solution to different culture bottles 610.

[0032] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions and variations can be made to the embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A high-efficiency incubator for plant tissue culture, comprising an incubator (1), a push handle (4) and four universal wheels (2), characterized in that: The incubator (1) has a door (3) hingedly connected to the front side thereof, an observation window being provided through the front side of the door (3), an observation window (5) being fixedly connected to the inner wall of the observation window, a culture mechanism (6) and a ventilation mechanism (7) being arranged inside the incubator (1), the ventilation mechanism (7) being located above the culture mechanism (6); the culture mechanism (6) comprises a fixed component and a rotating component, the fixed component being located above the rotating component; The rotating assembly comprises a first motor (608), the bottom surface of the first motor (608) being fixedly connected to the inner bottom surface of the incubator (1), and a connecting block (609) and a fixing block (607) being arranged above the first motor (608); The fixing component comprises a culture plate (601), the top surface of the culture plate (601) is fixedly connected to a ring plate (602), two clamps (603) are arranged inside the ring plate (602), the two clamps (603) are symmetrically arranged, a protective plate (604) is arranged on the inner side of the clamp (603), a culture bottle (610) is arranged between the two clamps (603), a pull rod (605) is arranged on the outer side of the clamp (603), and a spring (606) is sleeved on the surface of the pull rod (605).

2. The high-efficiency incubator for plant tissue culture according to claim 1, characterized in that: The top end surface of the output rod of the first motor (608) is fixedly connected to the bottom surface of the connecting block (609); a sliding groove matching the connecting block (609) is formed at the bottom surface of the fixing block (607); the top end surface of the connecting block (609) slides through the bottom surface of the fixing block (607) and extends into the inside of the sliding groove.

3. The high-efficiency incubator for plant tissue culture according to claim 1, characterized in that: The inner wall of the fixing block (607) is threadedly connected to the inner wall of the connecting block (609) via bolts, and the top surface of the fixing block (607) is fixedly connected to the bottom surface of the culture plate (601).

4. The high-efficiency incubator for plant tissue culture according to claim 1, characterized in that: The inner side surface of the clamping plate (603) is fixedly connected to the outer side surface of the protective plate (604), the inner side surface of the protective plate (604) is extruded and connected to the surface of the culture bottle (610), and the protective plate (604) is made of rubber.

5. The high-efficiency incubator for plant tissue culture according to claim 1, characterized in that: The outer side surface of the clamping plate (603) is fixedly connected to the inner end surface of the pull rod (605), the outer end surface of the pull rod (605) slides through the inner side surface of the ring plate (602) and extends to the outside of the ring plate (602), the inner end surface of the spring (606) is fixedly connected to the outer side surface of the clamping plate (603), and the outer end surface of the spring (606) is fixedly connected to the inner side surface of the ring plate (602).

6. The high-efficiency incubator for plant tissue culture according to claim 1, characterized in that: The right side of the push handle (4) is fixedly connected to the left side of the incubator (1), and the top surfaces of the four universal wheels (2) are fixedly connected to the bottom surface of the incubator (1).

7. The high-efficiency incubator for plant tissue culture according to claim 1, characterized in that: The ventilation mechanism (7) comprises a ventilation frame (701), the bottom end surface of the ventilation frame (701) is fixedly penetrated through the top surface of the incubator (1) and extends into the interior of the incubator (1), a second motor (702) is fixedly connected to the top surface of the interior of the ventilation frame (701), a fan (703) is fixedly connected to the bottom end surface of an output rod of the second motor (702), a ventilation opening is formed through the top surface of the ventilation frame (701), a first filter screen (704) is arranged above the ventilation opening, an air outlet is formed through the bottom surface of the incubator (1), a second filter screen (706) is fixedly connected to the inner wall of the air outlet, and the first filter screen (704) is a metal filter screen.

8. The high-efficiency incubator for plant tissue culture according to claim 7, characterized in that: The top surface of the ventilation frame (701) is provided with a placement groove, the inner wall of the placement groove is fixedly connected to a magnetic attraction strip (705), and the top surface of the magnetic attraction strip (705) is magnetically connected to the bottom surface of the first filter screen (704).

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