Microminiature insect smell induction and smell selection behavior observation device

Abstract

A microminiature insect smell induction and smell selection behavior observation device is characterized in that a smell induction device comprises a feeding chamber (1) and connecting pipes connected to the two ends of the feeding chamber (1), and the outer sides of the connecting pipes are connected with a smell storage chamber; air holes (15) are formed in the top of the feeding room (1), and a solution delivery device is arranged on the front; each connecting pipe is composed of a high-density sponge hollow sealing plug and a plastic sleeve with one end open and the other end provided with a breathable gauze element. The smell storage chamber is composed of a smell storage chamber body and a closed silica gel plug or a hollow silica gel plug, the two ends of the smell storage chamber body are open, and a breathable gauze is arranged in the center in the smell storage chamber body to prevent the trichogramma ovipositor from touching smell substances. The device is simple in structure, is suitable for smell research of trichogramma, has the advantages of high efficiency and labor saving, and can obviously improve the experiment efficiency.

Description

Technical Field

[0001] The utility model belongs to the field of agricultural plant protection, especially an insect technology, specifically a micro-insect odor induction and olfactory selection behavior observation device. Background Technique

[0002] Insects perceive various chemical substances in a complex environment through their sensitive olfactory systems, thus completing key physiological activities such as locating hosts, foraging, and finding egg-laying sites. In this process, olfactory-related genes play a crucial role.

[0003] Trichogramma Trichogramma spp, with a body length of 0.5 - 1 mm, is a micro-egg parasitoid wasp of the family Trichogrammatidae in the order Hymenoptera and is widely used in the biological control of agricultural pests. Although the research on Trichogramma has been relatively in-depth, due to its tiny size and flight ability, escape and hybridization often occur during breeding and scientific research. Currently, the research on the chemical ecology of Trichogramma mostly stays at the level of behavior observation, mainly using a four-wall olfactometer to study olfactory selection behavior. However, when operating this instrument, the upper side cover needs to be completely opened, there is a risk of Trichogramma escaping, and the instrument is too large, making experimental operations inconvenient. The patent application with the publication number CN116746553 discloses an insect olfactory selection behavior research device and its research method, including a T-shaped tube, a first test bottle, a second test bottle, and a sample bottle. Although olfactory selection behavior can be observed, it cannot ensure gas circulation, and it is difficult to take out the remaining Trichogramma in the bottle.

[0004] Studying the induction effect of exogenous odor substances on the expression of olfactory genes is of great significance, which can provide a basis for understanding the insect behavior regulation mechanism and analyzing the molecular mechanism of insects' adaptation to the environment. Currently, the research on the chemical ecology of Trichogramma mainly focuses on the behavioral level, and the research on its olfactory-related genes is relatively scarce. There is still a lack of reasonable devices and methods for studying the changes in related genes and related biological characteristics of Trichogramma after being stimulated by exogenous odors, and for observing olfactory selection behavior. Content of the Utility Model

[0005] The purpose of the utility model is to address the problem that the lack of relevant devices for observing the odor induction and olfactory selection behavior of micro-insects affects the development of related research work. It designs a micro-insect odor induction and olfactory selection behavior observation device and method with a simple structure, labor-saving, and high efficiency, which can be used for the research related to the olfaction of Trichogramma.

[0006] One of the technical solutions of the utility model is:

[0007] A micro-insect odor induction observation device, characterized in that it includes a breeding chamber 1, connecting pipes are symmetrically connected to one end or both ends of the breeding chamber 1, and an odor storage chamber is connected to the outer end of the connecting pipe; there is a threaded ventilation hole 15 above the breeding chamber 1, a ventilation screen 22 is covered on the ventilation hole, and a plastic lid 23 for closing is screwed on the ventilation hole 15; a delivery device for delivering solution or food is provided on the front of the breeding chamber 1; the delivery device includes a hollow delivery pipe 13, the hollow delivery pipe 13 can pass through a silicone plug 12 fixed on the front of the breeding chamber 1 and extend into the breeding chamber 1, and absorbent cotton 28 is added to the end of the hollow delivery pipe 13 extending into the breeding chamber. When delivery is not required, the hollow delivery pipe 13 is pulled out, and a sealing plug 29 is inserted to seal the through hole formed by pulling out the hollow delivery pipe 13; the connecting pipe is composed of a hollow airtight plug and a plastic sleeve that can prevent the escape of micro Trichogramma and ensure the smooth taking of experimental materials. One end of the plastic sleeve is provided with an opening, and the other end is provided with a ventilation screen; the odor storage chamber is composed of an odor storage chamber and a sealing silicone plug; the odor storage chamber has openings at both ends, and there is a ventilation screen in the middle to prevent the ovipositor of Trichogramma from touching the odor substance.

[0008] The second technical solution of the present utility model is:

[0009] A micro-insect olfactory selection behavior observation device, characterized in that it includes a breeding chamber 1, connecting pipes are symmetrically connected to one end or both ends of the breeding chamber 1, and an odor storage chamber is connected to the outer end of the connecting pipe; there is a threaded ventilation hole 15 above the breeding chamber 1, a ventilation screen 22 is covered on the ventilation hole, and a plastic lid 23 for closing is screwed on the ventilation hole 15; a delivery device for delivering solution or food is provided on the front of the breeding chamber 1; the delivery device includes a hollow delivery pipe 13, the hollow delivery pipe 13 can pass through a silicone plug 12 fixed on the front of the breeding chamber 1 and extend into the breeding chamber 1, and absorbent cotton 28 is added to the end of the hollow delivery pipe 13 extending into the breeding chamber. When the solution is not required to be delivered, the delivery device 14 can be replaced with a sealing plug 29 for sealing; the connecting pipe is composed of a hollow airtight plug and a plastic sleeve that can prevent the escape of micro Trichogramma and ensure the smooth taking of experimental materials. One end of the plastic sleeve is provided with an opening, and the other end is provided with a ventilation screen; the odor storage chamber is composed of an odor storage chamber and a hollow silicone plug, and the hollow silicone plug is used to externally connect a uniform air flow to ensure the air circulation of the whole device; the odor storage chamber has openings at both ends, and there is a ventilation screen in the middle to prevent the ovipositor of Trichogramma from touching the odor substance.

[0010] The breeding chamber 1 and the odor storage chamber are on the same level; the breeding chamber 1 and the odor storage chamber are cuboids for stable placement.

[0011] The breeding chamber 1, the hollow delivery pipe 13, the plastic sleeve, and the odor storage chamber are all made of plastic or glass.

[0012] The sealing plug is a high-density sponge product.

[0013] The aperture of the breathable gauze is 1000 meshes, which can not only effectively prevent trichogramma from passing through, but also keep the air and the odor of volatile odor substances flowing.

[0014] The silica gel plug 12 is used to fix the delivery device to ensure its stability in the breeding room, and the sealing plug 29 is also made of silica gel; the closed silica gel plug is used for the sealing of the closed space induced by exogenous odor to avoid the influence of other odors; the hollow silica gel plug is used for the odor behavior selection experiment, and is externally connected to a ventilation device to ensure air circulation.

[0015] The plastic lid 23 has threads and needs to be tightened during the study of the influence of exogenous odor substances on olfactory-related odor substances to ensure a closed environment and avoid other air pollution.

[0016] The beneficial effects of the present utility model are as follows:

[0017] The two silica gel plugs of the present utility model can be used for two different studies. When using the closed silica gel plug 8, the closed silica gel plug 9 and the plastic lid 23, scientific research on odor induction of micro-insects can be carried out; when using the hollow silica gel plug 10 and the hollow silica gel plug 11, scientific research on the observation of olfactory selection behavior of micro-insects can be carried out.

[0018] The present utility model realizes the isolation of the odor source, and ensures that scientific research such as odor induction and olfactory behavior selection observation can be carried out on trichogramma without contacting odor substances.

[0019] Each part of the present utility model can be disassembled, the internal channels of the device are reasonable, and it is easy to clean.

[0020] The structure of the present utility model is simple, suitable for the olfactory research of trichogramma, and has the advantages of high efficiency and labor saving, and can significantly improve the experimental efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic structural diagram of the present utility model for odor induction research.

[0022] Figure 2 It is a schematic structural diagram of the present utility model for observing olfactory selection behavior research.

[0023] Figure 3 It is an exploded schematic diagram of the present utility model.

[0024] Figure 4 Schematic diagrams of the dimensions of each part.

[0025] In the figure: 1. Rearing chamber; 2. First hollow sealing plug; 3. Second hollow sealing plug; 4. First plastic sleeve; 5. Second plastic sleeve; 6. First odor storage chamber; 7. Odor storage chamber; 8. First silica gel plug; 9. Second silica gel plug; 10. First hollow silica gel plug; 11. Second hollow silica gel plug; 12. Silica gel plug; 13. Hollow delivery tube; 14. Delivery device; 15. Vent hole; 16. First cavity; 17. Second cavity; 18 - 22. Breathable gauze; 23. Plastic lid; 24. First connecting tube; 25. Second connecting tube; 26. First odor storage room; 27. Second odor storage room; 28. Absorbent cotton; 29. Sealing plug; 30. Rearing device. Detailed implementation manners

[0026] The following further describes the present utility model in conjunction with the accompanying drawings and embodiments. Embodiment

[0027] As Figure 1 , 3 , shown in 4.

[0028] A micro - sized insect odor induction observation device includes a rearing chamber 1. One end or both ends of the rearing chamber 1 are symmetrically connected with connecting tubes. In this embodiment, an example is given with one connecting tube connected to each end. Specifically in implementation, those skilled in the art can remove the connecting tube and related accessories at one end according to needs. The outer end of each connecting tube is connected with an odor storage room; The assembly drawing of the observation device in this embodiment is as Figure 1 shown, and the exploded view is as Figure 3 shown. The dimensions of each component can be referred to Figure 4As shown in the figure. The observation device includes a breeding chamber 1, a first connecting pipe 24, a second connecting pipe 25, a first odor storage chamber 26, and a second odor storage chamber 27. The breeding chamber 1 is a cuboid with an open end at both ends, with a length, width, and height of 12 cm, 2 cm, and 2 cm respectively. There is a threaded ventilation hole 15 with a diameter of 1.6 cm above the breeding chamber 1. The ventilation hole is covered with a ventilation gauze 22, and the ventilation hole 15 can be sealed tightly with a plastic lid 23; A device for delivering solution (food) is provided on the front of the breeding chamber 1; The device for delivering the solution includes a hollow delivery pipe 13 with a length of 3.5 cm and a diameter of 0.2 cm. The hollow delivery pipe 13 passes through a silica gel plug 12 and enters the breeding chamber 1. The bottom of the hollow delivery pipe 13 is absorbent cotton 28, which is the breeding end. Both the first connecting pipe 24 and the second connecting pipe 25 have a hollow sealing plug 2, 3 and plastic sleeves 4, 5. The hollow sealing plug 2 and the hollow closing plug 3 are high-density sponges in the shape of a cube with a side length of 2 cm, and there are cylindrical cavities 16, 17 with a diameter of 1.6 cm inside; The plastic sleeves 4 and 5 with a diameter of 1.6 cm are nested in the cavities, and a ventilation gauze is installed on the side away from the breeding chamber 1, and the aperture size of the gauze is 1000 mesh or above. Both sides of the hollow sealing plug 2 or 3 are respectively connected to the breeding chamber 1 and the odor storage chambers 6, 7. The odor storage chamber 6 and the odor storage chamber 7 are cuboids with an open end at both ends, with a length, width, and height of 3 cm, 2 cm, and 2 cm respectively. There are ventilation gauzes 20, 21 with a side length of 2 cm inside to prevent the ovipositor of Trichogramma from touching the odor substances. The odor storage chambers 6 and 7 are externally connected to sealing silica gel plugs 8, 9.

[0029] When conducting scientific research applications on odor induction of micro-insects, first open the sealing silica gel plugs 8 and 9 to put in the odor substances, and then install the sealing silica gel plugs 8, 9; Next, put Trichogramma into the breeding chamber 1, and connect the first connecting pipe 24 and the second connecting pipe 25 in time to prevent Trichogramma from escaping. Seal the ventilation hole 15 at the upper end of the breeding chamber with a plastic lid 23 to ensure that there is no interference from other odor substances. After odor induction for different durations, refrigerate the device for a certain time, and take out Trichogramma for subsequent related scientific research. Embodiment

[0030] As Figure 2 、 3 、4 shown.

[0031] A micro-insect olfactory selection behavior observation device, including a breeding chamber 1, and one or both ends of the breeding chamber 1 are symmetrically connected with connecting pipes. In this embodiment, an example of connecting one connecting pipe at each end is used for illustration. Specifically, those skilled in the art can remove the connecting pipe and related accessories at one end according to needs. The outer end of each connecting pipe is connected with an odor storage chamber; The assembly drawing of the olfactory selection behavior observation device in this embodiment is as Figure 2 shown, and the explosion diagram is as Figure 3 shown, and the dimensions of each component can be referred toFigure 4 As shown in the figure, it includes a breeding chamber 1, a first connecting pipe 24, a second connecting pipe 25, a first odor storage chamber 26, and a second odor storage chamber 27. The breeding chamber 1 is a cuboid with open ends, having a length of 12 cm, a width of 2 cm, and a height of 2 cm. Above the breeding chamber 1, there is a threaded ventilation hole 15 with a diameter of 1.6 cm, which is covered with a breathable gauze 22. The ventilation hole 15 can be sealed tightly with a plastic lid 23. On the front of the breeding chamber 1, there is a device for delivering solution (food). The device for delivering solution includes a hollow delivery pipe 13 with a length of 3.5 cm and a diameter of 0.2 cm. The hollow delivery pipe 13 passes through a silica gel plug 12 and enters the breeding chamber 1. The bottom of the hollow delivery pipe 13 is absorbent cotton 28, which is the feeding end. Both the first connecting pipe 24 and the second connecting pipe 25 have a hollow sealing plug 2, 3 and plastic sleeves 4, 5. The hollow sealing plug 2 and the hollow closing plug 3 are cube-shaped high-density sponges with a side length of 2 cm, and there are cylindrical cavities 16, 17 with a diameter of 1.6 cm inside. The plastic sleeves 4 and 5 with a diameter of 1.6 cm are nested in the cavities, and a breathable gauze is installed on the side away from the breeding chamber 1, and the aperture size of the gauze is 1000 mesh or above. The two sides of the hollow sealing plug 2 or 3 are respectively connected to the breeding chamber 1 and the odor storage chambers 6, 7. The odor storage chamber 6 and the odor storage chamber 7 are cuboids with open ends, having a length of 3 cm, a width of 2 cm, and a height of 2 cm, and there are breathable gauzes 20, 21 with a side length of 2 cm inside to prevent the ovipositor of Trichogramma from touching the odor substances. The odor storage chamber 6 and the odor storage chamber 7 are externally connected to hollow silica gel plugs 10, 11.

[0032] When conducting scientific research applications for observing the olfactory selection behavior of micro-insects, the hollow silica gel plugs 10 and 11 can be used for scientific research on observing the olfactory selection behavior of micro-insects. When conducting the application, first open the hollow silica gel plugs 10 and 11 and put in the odor substances, then install the hollow silica gel plugs 10, 11 and connect the ventilation device; use a sealing plug 29. Then, put Trichogramma into the breeding chamber 1, and promptly connect the first connecting pipe 24 and the second connecting pipe 25 to prevent Trichogramma from escaping. Open the ventilation hole 15 at the upper end of the breeding chamber to ensure the air circulation inside the device, and record the selection duration and selection results of Trichogramma for later analysis and research.

[0033] When using the above two applications, the following points need to be noted: The device should be placed in an environment with constant temperature, constant humidity, stable light source and no other interfering colors or light sources around to avoid interference from external factors and ensure the randomness of the selection of Trichogramma. Since Trichogramma has phototaxis, it is recommended to use a light-emitting board with a unified light source as the bottom plate or place it in the dark. After each use, the inside of the device needs to be wiped with alcohol or clean water for cleaning, and it can be used again after being placed in a cool and ventilated place to dry.

[0034] The utility model is applicable not only to Trichogramma, but also to most micro and small insects, such as Pteromalus puparum, Encyrtidae, thrips, etc.

[0035] The parts not involved in the utility model can be realized by the prior art or improved by combining the prior art.

Claims

1. A micro insect odor induction device, characterized in that: The invention comprises a feeding room (1), wherein two ends of the feeding room (1) are symmetrically connected to connecting pipes, and the outer ends of the connecting pipes are connected to an odor storage chamber; a threaded air hole (15) is provided above the feeding room (1), the air hole is covered with an air-permeable gauze (22), and a sealing plastic cover (23) is screwed onto the air hole (15); a delivery device (14) for delivering solution or food is provided on the front of the feeding room (1); the delivery device (14) comprises a hollow delivery tube (13), the hollow delivery tube (13) can pass through a silicone plug (12) fixed on the front of the feeding room (1) and extend into the feeding room (1), and the hollow delivery tube (13) can pass through a silicone plug (12) fixed on the front of the feeding room (1) and extend into the feeding room (1). One end of the delivery tube (13) extending into the breeding room is equipped with absorbent cotton (28). When delivery is not needed, the hollow delivery tube (13) is pulled out, and a sealing plug (29) is inserted to seal the through hole formed by pulling out the hollow delivery tube (13). The connecting tube is composed of a hollow sealing plug and a plastic sleeve that can prevent the escape of micro-sized trichogrammatids and ensure smooth access to experimental materials. One end of the plastic sleeve is provided with an opening, and the other end is provided with a breathable gauze. The odor storage chamber is composed of an odor storage chamber and a closed silicone plug. The odor storage chamber is open at both ends, and a breathable gauze is provided in the center of the interior to prevent the trichogrammatid ovipositor from touching the odor substance.

2. A device for observing the olfactory selection behavior of micro-insects, characterized in that: The invention comprises a feeding room (1), wherein two ends of the feeding room (1) are symmetrically connected to connecting pipes, and the outer ends of the connecting pipes are connected to an odor storage chamber; a threaded air hole (15) is provided above the feeding room (1), the air hole is covered with an air-permeable gauze (22), and a plastic cover (23) for sealing is screwed onto the air hole (15); a delivery device (14) for delivering solution or food is provided on the front of the feeding room (1); the delivery device comprises a hollow delivery tube (13), the hollow delivery tube (13) can pass through a silicone plug (12) fixed on the front of the feeding room (1) and extend into the feeding room (1); the hollow delivery tube (13) ) is provided with absorbent cotton (28) at one end extending into the breeding room; when there is no need to deliver the solution, the delivery device (14) can be replaced with a sealing plug (29) for sealing; the connecting tube is composed of a hollow sealing plug and a plastic sleeve that can prevent the escape of micro-trichogrammatids and ensure the smooth removal of experimental materials, one end of the plastic sleeve is provided with an opening, and the other end is provided with a breathable gauze; the odor storage chamber is composed of an odor storage chamber and a hollow silicone plug, the hollow silicone plug is used to connect to a uniform airflow to ensure air circulation throughout the device; the odor storage chamber is open at both ends, and there is a breathable gauze in the center to prevent the ovipositor of the trichogrammatids from touching the odorous substance.

3. The observation device according to claim 1 or 2, characterized in that: The breeding room (1) and the odor storage room are both at the same level; the breeding room (1) and the odor storage room are rectangular parallelepipeds so as to be stably placed.

4. The observation device according to claim 1 or 2, characterized in that: The breeding room (1), the hollow delivery tube (13), the plastic sleeve, and the odor storage room are all made of plastic or glass.

5. The observation device according to claim 1 or 2, characterized in that: The sealing plug (29) is a high-density sponge product.

6. The observation device according to claim 1 or 2, characterized in that: The aperture of the breathable gauze is 1000 meshes, which can effectively prevent trichogrammatids from passing through and maintain the circulation of air and odors emitted by odorous substances.

7. The observation device according to claim 1 or 2, characterized in that: The silicone plug (12) is used to fix the delivery device to ensure its stability in the breeding room, and the sealing plug (29) is also made of silicone. The closed silicone plug is used to seal the closed space induced by exogenous odors to avoid the influence of other odors. The hollow silicone plug is used in the odor behavior selection experiment and is connected to an external ventilation device to ensure air circulation.

8. The observation device according to claim 1 or 2, characterized in that: The plastic cover (23) has threads, and needs to be tightened when studying the effects of exogenous odor substances on olfactory-related odor substances to ensure a closed environment and avoid other air pollution.

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