Semi-automatic sugar chain extractor

Abstract

The utility model discloses a semi-automatic carbohydrate chain extractor, including display screen, controller and support base, the lower end of display screen is equipped with the controller, the bottom of controller is fixedly connected with support base, one side of the upper end of support base is fixedly connected with limit shell frame, and the limit shell frame is fixedly connected with the support base. A moving air cylinder is slidably connected to the upper end of the limiting shell frame, a sampling needle is fixedly connected to the bottom of the moving air cylinder, a first fluted disc is rotatably connected to the upper portion of the center of the supporting base, the side end of the first fluted disc is connected with a second fluted disc in a meshed mode, and a bearing supporting plate is connected to the upper end of the second fluted disc in a penetrating mode; and a motor is installed at the upper end of the bearing supporting plate and drives the second fluted disc to be rotationally connected, a protective annular frame is fixedly connected to one side of the bearing supporting plate, and a rotating disc is rotationally connected to the lower portion of the center of the protective annular frame. The utility model relates to a semi-automatic sugar chain extractor, which realizes the purpose of positioning extraction through the arrangement of the structure.

Description

Technical Field

[0001] The utility model relates to the technical field of glycan extraction, in particular to a semi-automatic glycan extractor. Background Technique

[0002] Glycoproteins are mainly divided into two categories: N-glycoproteins and O-glycoproteins according to the glycan structure (N-linked glycan or O-linked glycan) and glycosylation sites. To extract glycans from glycoproteins, the target glycoprotein must be purified first. Glycoproteins are mostly located on the cell surface (belonging to membrane proteins) and have poor water solubility. Their effective extraction is one of the bottlenecks in the study of glycan structures in glycomics. In the past, it was often used to prepare plasma membranes, and then use detergents such as CHAPS to release or Triton X-114 phase separation, organic solvent extraction, etc. to extract crude glycoproteins, and then separate and purify the crude glycoproteins by methods such as dialysis, ultrafiltration, electrophoresis, and chromatography. However, the obtained products are basically mixtures of glycans of mixed proteins. If a single glycoprotein glycan is to be obtained, the target glycoprotein must be effectively separated and purified. At present, the IP method is often used to separate a mixed solution of the target glycoprotein, antibody, and some other non-specific acting proteins from cell or tissue lysates, and then the mixed solution containing the target glycoprotein is separated by PAGE electrophoresis. Then, the gel band of the target protein is cut off, and the target glycoprotein glycan is extracted after enzymatic digestion or acid hydrolysis in the gel, or the separated protein is transferred from the gel to a PVDF membrane, and then the glycan is extracted from the PVDF membrane, that is, the glycan is extracted with the PVDF membrane as a carrier.

[0003] According to the Chinese patent number CN202021455735.0, a multi-sample universal nucleic acid extractor is provided, including an extractor body, a control screen, and ventilation holes. The outside of the extractor body is rotatably connected with a cover plate. An observation window is arranged at the middle position on the outside of the cover plate. A fixing block is arranged below the outside of the cover plate. A ventilation hole is arranged on one side of the extractor body, and the other side of the extractor body is connected with a control screen through a connecting rod.

[0004] However, the current glycan extractor has the following problems: there is a phenomenon that it is not convenient for efficient cooperative sample conduction, which will cause certain inconvenience and needs to be improved. Content of the Utility Model

[0005] The purpose of the utility model is to provide a semi-automatic glycan extractor to solve the problems put forward in the above background technique.

[0006] To achieve the above purpose, the utility model provides the following technical solutions:

[0007] A semi-automatic glycan extractor includes a display screen, a controller, and a support base. The controller is installed at the lower end of the display screen, and the support base is fixedly connected to the bottom of the controller;

[0008] One side of the upper end of the support base is fixedly connected with a limit shell frame. A moving cylinder is slidably connected to the upper end of the limit shell frame. A sampling needle is fixedly connected to the bottom of the moving cylinder.

[0009] The upper center of the support base is rotatably connected with a first gear disc. The side end of the first gear disc is meshed and connected with a second gear disc. The upper end of the second gear disc is connected through and supported by a support plate. And a motor is installed on the upper end of the support plate. The motor drives the second gear disc to rotate. One side of the support plate is fixedly connected with a protective annular frame. The lower center of the protective annular frame is rotatably connected with a rotating disc.

[0010] Specifically, the rotating disc is rotatably connected to the center of the protective annular frame through the drive of the first gear disc.

[0011] Specifically, the motor drives the first gear disc to rotate through the second gear disc.

[0012] Specifically, a storage groove is formed on the side of the rotating disc.

[0013] Specifically, the display screen, the controller are electrically connected to the moving cylinder and the motor.

[0014] Specifically, a groove is formed on one side of the protective annular frame, and the groove communicates with the rotating disc.

[0015] Compared with the prior art, the beneficial effects of the present utility model are as follows:

[0016] First, by installing a display screen and a controller, the display screen and the controller can be used for control. The setting of the support base facilitates the load-bearing connection work of the overall structure. The limit shell frame is used for the limit support of the moving cylinder. Through driving, the moving cylinder can be slidably connected on the limit shell frame, so that the moving cylinder can drive the sampling needle to displace, make contact with the test tube, and facilitate the extraction work of the sample.

[0017] Second, by installing a motor, the motor can drive the second gear disc to rotate, acting on the first gear disc, so that the first gear disc drives the rotating disc to rotate. The protective annular frame and the support plate are fixed, and a groove is formed on the side end of the protective annular frame, which is convenient for placing the test tube rack. Through the conduction of the rotating disc, the test tube rack reaches the lower part of the sampling needle for positioning and extraction work. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the main structure of the present utility model;

[0019] Figure 2 It is a side view of the main body of the present utility model;

[0020] Figure 3 This is the exploded view of the main body of the present utility model.

[0021] In the figure: 1 - display screen; 2 - controller; 3 - support base; 4 - sampling needle; 5 - limit housing frame; 6 - moving cylinder; 7 - first gear disk; 8 - second gear disk; 9 - rotating disk; 10 - protective annular frame; 11 - supporting plate; 12 - motor. Specific embodiments

[0022] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all of the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model.

[0023] Please refer to Figures 1-3 , the present utility model provides a technical solution: a semi-automatic sugar chain extractor, including a display screen 1, a controller 2 and a support base 3. The controller 2 is installed at the lower end of the display screen 1, and the support base 3 is fixedly connected to the bottom of the controller 2;

[0024] On one side of the upper end of the support base 3, a limit housing frame 5 is fixedly connected. A moving cylinder 6 is slidably connected to the upper end of the limit housing frame 5, and a sampling needle 4 is fixedly connected to the bottom of the moving cylinder 6. By installing the display screen 1 and the controller 2, the display screen 1 and the controller 2 can be controlled. The setting of the support base 3 facilitates the load-bearing connection work of the overall structure. The limit housing frame 5 is used for the limit support of the moving cylinder 6. The moving cylinder 6 can be slidably connected on the limit housing frame 5 through driving, so that the moving cylinder 6 can drive the sampling needle 4 to displace, making contact with the test tube, and facilitating the sample extraction work;

[0025] The first gear disk 7 is rotatably connected to the upper center of the support base 3. The side end of the first gear disk 7 is meshed with the second gear disk 8. The upper end of the second gear disk 8 is connected through the supporting plate 11, and a motor 12 is installed at the upper end of the supporting plate 11. The motor 12 drives the second gear disk 8 to rotate. One side of the supporting plate 11 is fixedly connected with a protective annular frame 10. The lower center of the protective annular frame 10 is rotatably connected with a rotating disk 9. By installing the motor 12, the motor 12 can drive the second gear disk 8 to rotate, acting on the first gear disk 7, so that the first gear disk 7 drives the rotating disk 9 to rotate. The protective annular frame 10 and the supporting plate 11 are fixed, and a groove is provided at the side end of the protective annular frame 10, which is convenient for placing the test tube rack. Through the conduction of the rotating disk 9, the test tube rack reaches the lower part of the sampling needle 4 for positioning extraction work.

[0026] The rotating disk 9 is rotationally connected at the center of the protective annular frame 10 driven by the first toothed disk 7. The motor 12 drives the first toothed disk 7 to be rotationally connected through the second toothed disk 8. A material storage groove is formed on the side of the rotating disk 9. The display screen 1, the controller 2 are electrically connected to the motion cylinder 6 and the motor 12. A groove is formed on one side of the protective annular frame 10, and the groove communicates with the rotating disk 9.

[0027] Working principle: When working is required, the user installs the display screen 1 and the controller 2, and the display screen 1 and the controller 2 can be controlled. The setting of the support base 3 facilitates the load-bearing connection work of the overall structure. The limit housing frame 5 is used for the limit support of the motion cylinder 6. The motion cylinder 6 can be slidably connected on the limit housing frame 5 through driving, so that the motion cylinder 6 can drive the sampling needle 4 to displace, make the sampling needle 4 contact with the test tube, and facilitate the extraction work of the sample. By installing the motor 12, the motor 12 can drive the second toothed disk 8 to rotate, act on the first toothed disk 7, so that the first toothed disk 7 drives the rotating disk 9 to rotate. The protective annular frame 10 and the supporting plate 11 are fixed, and a groove is formed at the side end of the protective annular frame 10 to facilitate the placement of the test tube rack. Through the conduction of the rotating disk 9, the test tube rack reaches the lower part of the sampling needle 4 for positioning extraction work, and the work is completed.

[0028] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principle and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A semi-automatic sugar chain extractor, comprising a display screen (1), a controller (2) and a support base (3), characterized in that: A controller (2) is installed at the lower end of the display screen (1), and a supporting base (3) is fixedly connected to the bottom of the controller (2); A limiting shell frame (5) is fixedly connected to one side of the upper end of the support base (3); a moving cylinder (6) is slidably connected to the upper end of the limiting shell frame (5); and a sampling needle (4) is fixedly connected to the bottom of the moving cylinder (6); The central upper part of the support base (3) is rotatably connected to a first toothed disc (7), the side end of the first toothed disc (7) is meshedly connected to a second toothed disc (8), the upper end of the second toothed disc (8) is through-connected to a supporting support plate (11), and an electric motor (12) is installed at the upper end of the supporting support plate (11), the electric motor (12) drives the second toothed disc (8) to be rotatably connected, one side of the supporting support plate (11) is fixedly connected to a protective annular frame (10), and the central lower part of the protective annular frame (10) is rotatably connected to a rotating disc (9).

2. A semi-automatic sugar chain extractor according to claim 1, characterized in that: The rotating disk (9) is driven by the first toothed disk (7) to be rotationally connected at the center of the protective annular frame (10).

3. A semi-automatic sugar chain extractor according to claim 2, characterized in that: The motor (12) drives the first gear disc (7) to rotate through the second gear disc (8).

4. A semi-automatic sugar chain extractor according to claim 3, characterized in that: A material storage trough is provided on the side of the rotating disk (9).

5. A semi-automatic sugar chain extractor according to claim 4, characterized in that: The display screen (1), the controller (2), the motion cylinder (6), and the motor (12) are electrically connected.

6. A semi-automatic sugar chain extractor according to claim 5, characterized in that: A groove is provided on one side of the protective annular frame (10), and the groove is connected to the rotating disk (9).

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