Microorganism separation and purification device

Abstract

A microorganism separation and purification device is arranged on an experiment platform, a bayonet socket is arranged on the experiment platform, a plurality of bayonet grooves are formed in the bayonet socket from bottom to top, a culture dish operation table in sliding connection with the bayonet socket is arranged on the bayonet socket, a positioning groove is formed in the upper surface of the culture dish operation table, and the bottom face of the positioning groove is matched with a culture dish. The depth of the positioning groove is smaller than the height of the culture dish; an alcohol lamp group is arranged on one side of the culture dish operation table in the sliding connection direction, and the culture dish operation table can be close to or away from the alcohol lamp group in the horizontal direction. According to the utility model, the height of the culture dish can be adjusted according to the flame height of the alcohol lamp group by moving the position of the culture dish operation table in the horizontal direction of the bayonet socket, so that a molten culture medium is in a sterile state when being poured into the sterile culture dish, and a plurality of culture dishes do not need to be used as bases; the positions of the culture dishes below do not need to be frequently adjusted, and molten culture media can be conveniently and rapidly poured into the multiple culture dishes respectively.

Description

Technical Field

[0001] The utility model relates to the technical field of microorganism separation and purification, and specifically relates to a microorganism separation and purification device. Background Technique

[0002] Microorganism separation and purification is a basic technique in microbiology experiments. Its purpose is to isolate single microorganism species from a mixed microorganism population to obtain pure culture. Commonly used microorganism separation and purification methods include the dilution pour plate method, spread plate method, streak plate method, and dilution shake tube method. Among them, the spread plate method is more commonly used. The steps are generally as follows: first, pour the melted culture medium into a sterile petri dish to make a sterile plate. After cooling and solidifying, drop a certain amount of microorganism suspension on the surface of the plate, and then use a sterile glass spreader to evenly disperse the bacterial liquid over the entire surface of the plate. After culturing, pick a single colony.

[0003] During the process of pouring the melted culture medium into a sterile petri dish, due to the need for aseptic operation beside an alcohol lamp, multiple petri dishes are generally stacked up and down and taken out for use at the same time. The height of a single petri dish is generally between 15 - 25 millimeters. This makes the height of multiple stacked petri dishes being too high or too low affect the use of the petri dishes by experimental operators. Generally, experimental operators will take out multiple stacked petri dishes and always use the top three petri dishes. After opening the top three petri dishes in turn to add the culture medium, the top three petri dishes are taken off at the same time, and the multiple petri dishes below are not used as a base. Then, three new petri dishes are placed on the multiple petri dishes below. In this way of use, the petri dishes below are prone to dislocation or tipping, and it is necessary to frequently straighten the positions of the multiple petri dishes below to avoid the multiple petri dishes as a base from tilting and affecting the operation of the top three petri dishes; moreover, the height of the petri dishes used in each experiment is different, and the number of petri dishes used as height increasing below is different, making it inconvenient to inject the culture medium into the petri dishes. Content of the Utility Model

[0004] To solve the technical problems existing in the above background technique, the utility model provides a microorganism separation and purification device.

[0005] The technical solution of the utility model is as follows:

[0006] A microorganism separation and purification device is arranged on an experimental platform. There is a socket on the experimental platform. The socket is provided with a plurality of insertion slots from bottom to top. There is a petri dish operation table slidably connected to the socket. The sliding direction of the petri dish operation table is horizontal and is consistent with the length direction of the insertion slots;

[0007] The upper surface of the petri dish operation table is provided with a positioning groove. The bottom surface of the positioning groove is adapted to the petri dish, and the depth of the positioning groove is less than the height of the petri dish;

[0008] One side of the culture dish operating table along its sliding direction is provided with an alcohol lamp group. The culture dish operating table can move horizontally closer to or farther away from the alcohol lamp group, and the positioning groove is arranged near the middle position of the alcohol lamp group. The highest height that the positioning groove can reach is higher than the highest height of the alcohol lamp group.

[0009] First, place one or more culture dishes on the positioning groove, and by moving the position of the culture dish operating table along the horizontal direction in the socket, adjust the position of the culture dish relative to the alcohol lamp group on one side. By adjusting the position of the culture dish operating table in the insertion slot, the height of the culture dish can be adjusted according to the flame height of the alcohol lamp group, so that during the process of pouring the melted culture medium into the sterile culture dish, it is in a sterile state, and there is no need to use multiple culture dishes as bases, and there is no need to frequently straighten the position of the lower culture dish, which is convenient for quickly pouring the melted culture medium into multiple culture dishes respectively.

[0010] The specific structure of the above-mentioned socket is that the socket includes two symmetrically arranged seat bodies. The seat bodies are detachably connected to the experimental platform. A plurality of insertion slots are arranged on the seat bodies. The insertion slots of the two seat bodies have openings facing each other. The two ends of the culture dish operating table along its length direction are correspondingly slidably connected in the insertion slots of the two seat bodies.

[0011] To facilitate adjusting the position of the culture dish in the width direction of the insertion slot and quickly adjusting the distance between the culture dish and the alcohol lamp of the alcohol lamp group, the length of the culture dish operating table is set between the maximum vertical distance and the minimum vertical distance of the insertion slots of the two seat bodies, and the culture dish operating table can slide along the width direction of the insertion slot.

[0012] Regarding the structure of the culture dish operating table, the culture dish operating table includes an upper plate and a lower plate arranged parallel to each other up and down. The upper plate and the lower plate are connected by vertically arranged connecting plates. The positioning groove is arranged on the upper surface of the upper plate. The connecting plates are arranged on the side away from the alcohol lamp group, which is convenient for adjusting the position of the culture dish on the upper plate through the connecting plates. The lower plate is inserted and connected to the insertion slot. The vertical distance between the upper plate and the lower plate is greater than the height of the seat body, so that when the lower plate is located in the lowermost insertion slot, the upper plate is located above the base, and the height of the upper plate is slightly higher than the base, which is convenient for adjusting the height of the culture dish according to the alcohol lamp group, so that during the process of pouring the melted culture medium into the sterile culture dish, it is always in a sterile environment.

[0013] To limit the position of the culture dish operating table in the length direction of the insertion slot, a positioning rod is arranged on at least one seat body, and its length direction is consistent with the width direction of the insertion slot. One end of the positioning rod passes through the seat body and is inserted and connected to the lower plate.

[0014] The above is to facilitate adjusting the height of the culture dish operating table on the seat body and then adjusting the height of the culture dish. A through hole adapted to the positioning rod is correspondingly arranged outside each insertion slot.

[0015] To facilitate the adjustment of the position of the culture dish operation table along the length direction of the insertion slot, and ensure that the culture dish operation table does not disengage from the insertion slot after adjustment, multiple insertion holes are provided along the length direction of the insertion slot on the lower plate, and the insertion holes are adapted to the positioning rods.

[0016] To facilitate the quick reset of the positioning rod, a tension spring is sleeved on the positioning rod. One end of the tension spring is connected to the outer side surface of the seat body, and the other end is connected to the end of the positioning rod far from the culture dish operation table.

[0017] To facilitate the picking and placing of the culture dish, a picking and placing opening is provided in the middle of the upper plate on the side far from the alcohol lamp group, and the picking and placing opening is communicated with the positioning groove.

[0018] The beneficial effects of the present utility model are as follows;

[0019] During the process of pouring the melted culture medium into the sterile culture dish, in order to avoid the possible tipping over due to the relatively high stacking height of multiple culture dishes, and to facilitate the stable pouring of the culture medium into the upper culture dish, a culture dish operation table with adjustable height is provided. The culture dish operation table is used to place the culture dish, which can reduce the height of the culture dish, avoid the use of the most unstable base of the stacked culture dishes, and the culture dish operation table can move away from and close to the alcohol lamp group, so that it can always be in a suitable sterile environment when pouring the culture medium in the microbial isolation and purification;

[0020] To facilitate the adjustment of the position of the culture dish in the width direction of the insertion slot and quickly adjust the distance between the culture dish and the alcohol lamp of the alcohol lamp group, the length of the culture dish operation table is set between the maximum vertical distance and the minimum vertical distance of the insertion slots of the two seat bodies, and the culture dish operation table can slide along the width direction of the insertion slot;

[0021] To facilitate the adjustment of the position of the culture dish operation table along the length direction of the insertion slot, and ensure that the culture dish operation table does not disengage from the insertion slot after adjustment, multiple insertion holes are provided along the length direction of the insertion slot on the lower plate, and the insertion holes are adapted to the positioning rods. Description of the Drawings

[0022] In the drawings:

[0023] Figure 1 is a schematic structural diagram;

[0024] Figure 2 is Figure 1 the enlarged structural diagram at A in

[0025] Figure 3 is a schematic structural diagram of the culture dish operation table;

[0026] Figure 4 is a schematic structural diagram of the insertion seat;

[0027] Figure 5Schematic diagram of the installation structure of the petri dish operation table and the socket

[0028] The components represented by the reference numerals in the figure are as follows:

[0029] 1. Experimental platform; 2. Socket; 21. Socket body; 211. Through hole; 22. Insertion slot; 221. Opening; 3. Petri dish operation table; 31. Upper plate; 311. Positioning groove; 312. Pick-up and placement opening; 32. Lower plate; 321. Insertion hole; 33. Connecting plate; 4. Alcohol lamp group; 5. Positioning rod; 6. Tensile spring; 7. Petri dish Specific implementation mode

[0030] See Figure 1 and Figure 2 As shown, a microorganism isolation and purification device is arranged on the experimental platform 1. A socket 2 is provided on the experimental platform 1. The socket 2 includes two symmetrically arranged socket bodies 21. The socket body 21 is detachably connected to the experimental platform 1. A plurality of insertion slots 22 are provided on the two socket bodies 21 from bottom to top. The insertion slots 22 of the two socket bodies 21 have openings 221 that are oppositely arranged. The two ends of the petri dish operation table 3 in the length direction are correspondingly slidably connected in the insertion slots 22 of the two socket bodies 21. The sliding direction of the petri dish operation table 3 is horizontal and is consistent with the length direction of the insertion slot 22

[0031] See Figure 3 and Figure 5 As shown, a positioning groove 311 is provided on the upper surface of the petri dish operation table 3. The bottom surface of the positioning groove 311 is adapted to the petri dish 7, and the depth of the positioning groove 311 is less than the height of the petri dish 7

[0032] An alcohol lamp group 4 is provided on one side of the petri dish operation table 3 along its sliding direction. The alcohol lamp group 4 includes a plurality of alcohol lamps. The petri dish operation table 3 can approach or move away from the alcohol lamp group 4 in the horizontal direction, and the positioning groove 311 is arranged near the middle position of the alcohol lamp group 4. The highest height that the positioning groove 311 can reach is higher than the highest height of the alcohol lamp group 4

[0033] See Figure 3 and Figure 5As shown in the figure, the structure of the above-mentioned petri dish operating table 3 is that the petri dish operating table 3 includes an upper plate 31 and a lower plate 32 which are arranged parallel to each other up and down. The upper plate 31 and the lower plate 32 are connected by a vertically arranged connecting plate 33. The positioning groove 311 is arranged on the upper surface of the upper plate 31. The connecting plate 33 is arranged on the side far away from the alcohol lamp group 4, which is convenient for adjusting the position of the petri dish 7 on the upper plate 31 through the connecting plate 33. The lower plate 32 is inserted and connected with the insertion groove 22. The vertical distance between the upper plate 31 and the lower plate 32 is greater than the height of the seat body 21. When the lower plate 32 is located in the lowermost insertion groove 22, the upper plate 31 is located above the base, and the height of the upper plate 31 is slightly higher than that of the base, which is convenient for adjusting the height of the petri dish 7 according to the alcohol lamp group 4, so that during the process of pouring the melted culture medium into the sterile petri dish 7, it is always in a sterile environment.

[0034] See Figure 3 As shown in the figure, for the convenience of taking and placing the petri dish 7, a taking and placing opening 312 is provided in the middle of the side of the upper plate 31 far away from the alcohol lamp group 4. The taking and placing opening 312 is communicated with the positioning groove 311. The two right-angled corners of the taking and placing opening 312 far away from the alcohol lamp group 4 are set as rounded corners to avoid the experimenter bumping into the right-angled corners during operation and causing injuries.

[0035] The positions of the above two seat bodies 21 on the experimental platform 1 are not convenient to adjust. For the convenience of adjusting the position of the petri dish 7 in the width direction of the insertion groove 22 and quickly adjusting the distance between the petri dish 7 and the alcohol lamps of the alcohol lamp group 4, the length of the petri dish operating table 3 is set between the maximum vertical distance and the minimum vertical distance of the insertion grooves 22 of the two seat bodies 21, and the petri dish operating table 3 can slide along the width direction of the insertion groove 22.

[0036] The function realized by the above structure is as follows: First, place a single or multiple petri dishes 7 on the positioning groove 311, and adjust the position of the petri dish 7 away from one side of the alcohol lamp group 4 by moving the petri dish operating table 3 horizontally along the socket 2. Adjust the position of the petri dish operating table 3 in the insertion groove 22, and the height of the petri dish 7 can be adjusted according to the flame height of the alcohol lamp group 4, so that during the process of pouring the melted culture medium into the sterile petri dish 7, it is in a sterile state, and there is no need to use multiple petri dishes 7 as the base, and there is no need to frequently straighten the position of the lower petri dish 7, which is convenient for quickly pouring the melted culture medium into multiple petri dishes 7 respectively.

[0037] See Figure 4As shown, in order to limit the position of the culture dish operation platform 3 in the length direction of the plug-in slot 22, a positioning rod 5 is provided on at least one seat body 21, and its length direction is consistent with the width direction of the plug-in slot 22. One end of the positioning rod 5 passes through the seat body 21 and is plugged and connected with the lower plate 32. In order to facilitate the adjustment of the height of the culture dish operation platform 3 on the seat body 21, and then adjust the height of the culture dish 7, the outer side of each plug-in slot 22 is correspondingly provided with a through hole 211 adapted to the positioning rod 5, the axis of the through hole 211 is consistent with the width direction of the plug-in slot 22, and the axis of the through hole 211 is located in the middle of the plug-in slot 22, and the inner diameter of the through hole 211 is consistent with the inner diameter of the plug-in hole 321. In order to facilitate the adjustment of the position of the culture dish operation platform 3 in the plug-in slot 22 along its length direction, and the culture dish operation platform 3 will not be separated from the plug-in slot 22 after adjustment, the lower plate 32 is provided with a plurality of plug-in holes 321 along the length direction of the plug-in slot 22, and the plug-in holes 321 are adapted to the positioning rod 5.

[0038] See also Figure 4 and Figure 5 As shown, when adjusting the position of the culture dish operating table 3 along its length direction in the insertion groove 22, in order to facilitate the rapid resetting of the positioning rod 5, a tension spring 6 is sleeved on the positioning rod 5, and one end of the tension spring 6 is connected to the outer side surface of the seat body 21, and the other end is connected to the end of the positioning rod 5 away from the culture dish operating table 3.

Claims

1. A microbial separation and purification device, arranged on an experimental platform (1), characterized in that: The experimental platform (1) is provided with a plug seat (2), the plug seat (2) is provided with a plurality of plug slots (22) from bottom to top, the plug seat (2) is provided with a culture dish operating table (3) slidably connected thereto, and the sliding direction of the culture dish operating table (3) is horizontal and consistent with the length direction of the plug slots (22); The upper surface of the culture dish operating table (3) is provided with a positioning groove (311), the bottom surface of the positioning groove (311) is adapted to the culture dish (7), and the depth of the positioning groove (311) is less than the height of the culture dish (7); An alcohol lamp group (4) is provided on one side of the culture dish operating table (3) along the sliding direction thereof; the culture dish operating table (3) can be moved closer to or farther away from the alcohol lamp group (4) in the horizontal direction; and the positioning groove (311) is arranged near the middle position of the alcohol lamp group (4); the maximum height that the positioning groove (311) can reach is higher than the maximum height of the alcohol lamp group (4).

2. A microorganism separation and purification device according to claim 1, characterized in that: The plug-in seat (2) comprises two symmetrically arranged seat bodies (21), the seat bodies (21) are detachably connected to the experimental platform (1), a plurality of plug-in slots (22) are arranged on the seat bodies (21), the plug-in slots (22) of the two seat bodies (21) have openings (221) opened opposite to each other, and the two ends of the culture dish operating table (3) along its length direction are correspondingly slidably connected in the plug-in slots (22) of the two seat bodies (21).

3. A microorganism separation and purification device according to claim 2, characterized in that: The length of the culture dish operating platform (3) is between the maximum vertical spacing and the minimum vertical spacing of the plug-in slots (22) of the two base bodies (21), and the culture dish operating platform (3) can slide along the width direction of the plug-in slots (22).

4. A microorganism separation and purification device according to claim 1, characterized in that: The culture dish operating table (3) comprises an upper plate (31) and a lower plate (32) which are arranged in parallel up and down, the upper plate (31) and the lower plate (32) are connected by a vertically arranged connecting plate (33), the positioning groove (311) is arranged on the upper surface of the upper plate (31), the connecting plate (33) is arranged on a side away from the alcohol lamp group (4), the lower plate (32) is plug-connected with the plug-in groove (22), and the vertical spacing between the upper plate (31) and the lower plate (32) is greater than the height of the base (21).

5. A microorganism separation and purification device according to claim 4, characterized in that: At least one of the seat bodies (21) is provided with a positioning rod (5), the length direction of which is consistent with the width direction of the plug-in slot (22); one end of the positioning rod (5) passes through the seat body (21) and is plug-connected with the lower plate (32).

6. A microorganism separation and purification device according to claim 5, characterized in that: The outer side of each of the insertion slots (22) is correspondingly provided with a through hole (211) adapted to the positioning rod (5).

7. A microorganism separation and purification device according to claim 6, characterized in that: The lower plate (32) is provided with a plurality of plug holes (321) along the length direction of the plug slot (22), and the plug holes (321) are adapted to the positioning rod (5).

8. A microorganism separation and purification device according to claim 5, characterized in that: The positioning rod (5) is sleeved with a tension spring (6), one end of the tension spring (6) is connected to the outer side surface of the seat body (21), and the other end is connected to the end of the positioning rod (5) away from the culture dish operating table (3).

9. A microorganism separation and purification device according to claim 4, characterized in that: A take-in / take-out opening (312) is provided in the middle of the upper plate (31) on the side away from the alcohol lamp group (4), and the take-in / take-out opening (312) is communicated with the positioning groove (311).

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