Lung knock injury cell model construction device

Abstract

The utility model relates to the technical field of lung knock injury cell models, in particular to a lung knock injury cell model building device which comprises a test tube inversion frame and a gas release port, the gas release port is arranged under a lower port of the test tube inversion frame and connected with an air compressor through a gas release tube, and the air compressor is connected with the test tube inversion frame. The test tube inversion rack and the gas release port are fixedly installed on the support, the support is fixedly installed on the base, a gas pressure sensor is arranged between the gas release port and a lower port of the test tube inversion rack, and a test tube fastening mechanism is arranged on the test tube inversion rack. According to the utility model, the detonation environment can be stably simulated by accurately controlling the pressure released by the gas, and a reliable model is provided for in-depth study of lung detonation injured cells; the reliable test tube fixing mechanism effectively overcomes the influence of vibration on the result in the experiment process of holding the test tube by hand.

Description

Technical Field

[0001] The utility model relates to the technical field of lung blast injury cell models, and specifically relates to a device for constructing a lung blast injury cell model. Background Technique

[0002] The international situation is volatile, and the form of war has also undergone a fundamental transformation. Blast injuries caused by high-speed, high-energy, and high-explosive weapons have become the main types of injuries in modern wars. At the same time, sudden explosion incidents in daily industrial production and life (such as gas explosions and gas explosions) are also important causes of blast injuries. The incidence and mortality of blast injuries are high. It is reported that the incidence of blast injuries caused by various weapons in military conflicts is over 80%; the total mortality rate is as high as 73.7%. The lungs are the organs most vulnerable to injury in blast injuries, and the number of deaths caused by severe lung blast injuries accounts for 92.6% of the total number of deaths caused by blast injuries.

[0003] At present, there is a lack of in-depth molecular biology research on lung blast injuries. The main reason is the lack of a stable cell model. Therefore, there is an urgent need for a stable and reliable device for constructing a lung blast injury cell model to provide an effective solution to the defects of the existing technology. Content of the Utility Model

[0004] The purpose of the utility model is to provide a device for constructing a lung blast injury cell model to solve the problems raised in the above background technique.

[0005] To achieve the above purpose, the utility model provides the following technical solutions:

[0006] A device for constructing a lung blast injury cell model includes a test tube inverted rack and a gas release port. The gas release port is directly below the lower port of the test tube inverted rack. The gas release port is connected to an air compressor through a gas release pipe. Both the test tube inverted rack and the gas release port are fixedly installed on a bracket, and the bracket is fixedly installed on a base. A gas pressure sensor is arranged between the gas release port and the lower port of the test tube inverted rack, and a test tube fastening mechanism is arranged on the test tube inverted rack.

[0007] Furthermore, the test tube inverted rack is set as a cylindrical structure that is vertically through. A horizontally inward-extending limit retaining ring is arranged on the lower port of the test tube inverted rack, and the limit retaining ring is used to limit the port of the test tube.

[0008] Furthermore, the test tube fastening mechanism includes a group of retaining rods that are arranged on the upper port of the test tube inverted rack and extend vertically upward. A plurality of wedge-shaped blocks are arranged at equal intervals on the outer surface of the retaining rods. A plurality of fastening rings are sleeved outside the retaining rods, and the plurality of fastening rings are fixedly connected by connecting rods. The fastening rings are in clamping fit with the wedge-shaped blocks. When the fastening rings are pressed down, the wedge-shaped blocks are forced to retract inward, and the retaining rods clamp the test tube after retracting with the wedge-shaped blocks.

[0009] Further, a threaded port is provided on the upper port of the test tube inversion rack, and a threaded sleeve is rotatably connected to the bottom of the lowermost fastening ring. The threaded sleeve is threadedly connected to the threaded port.

[0010] Further, anti-slip threads are provided on the circumferential outer wall of the threaded sleeve.

[0011] Further, a buffer cushion layer is connected to the inner surface of the retaining rod.

[0012] Compared with the prior art, the beneficial effects of the present utility model are as follows:

[0013] 1. The present utility model can stably simulate the detonation environment by precisely controlling the pressure of gas release, providing a reliable model for in-depth research on lung blast injury cells; the reliable test tube fixing mechanism effectively overcomes the influence of vibration on the results during the experiment of holding the test tube by hand.

[0014] 2. The core of the test tube fastening mechanism in the present utility model lies in the clamping fit between the retaining rod and the fastening ring. The multiple wedge-shaped blocks provided on the retaining rod are in an outward-expanded state in the initial state. When multiple fastening rings are connected by connecting rods and pressed downward along the retaining rod, the inner wall of the fastening ring will squeeze the inclined surface of the wedge-shaped block, forcing the wedge-shaped block to contract inward. Due to the special shape of the wedge-shaped block, this extrusion will cause the distance between them to decrease, thereby enabling the entire retaining rod to form a clamping effect on the test tube.

[0015] 3. The upper port of the test tube inversion rack in the present utility model is designed with a threaded port, which is matched with the threaded sleeve at the bottom of the lowermost fastening ring. By rotating the threaded sleeve, the up and down movement of the fastening ring along the retaining rod can be precisely controlled, thereby realizing the adjustment of the clamping degree of the test tube. The anti-slip threads are provided on the circumferential outer wall of the threaded sleeve, increasing the friction during rotation, facilitating the operation of the user and preventing hand slippage.

[0016] 4. In the present utility model, a buffer cushion layer is connected to the inner surface of the retaining rod. This layer of buffer material plays a buffering role when the test tube is clamped, capable of absorbing part of the impact brought by the clamping force and protecting the integrity of the test tube and its contents. Description of the Drawings

[0017] Figure 1 It is a schematic structural diagram of a device for constructing a lung blast injury cell model;

[0018] Figure 2 It is a schematic structural diagram of the test tube inversion rack;

[0019] Figure 3 It is a schematic exploded view of a device for constructing a lung blast injury cell model;

[0020] Figure 4 It is a schematic structural diagram of the test tube fastening mechanism;

[0021] Figure 5 Schematic diagram of the structure at the port of the test tube inversion rack.

[0022] In the figure: 1. Gas release tube; 2. Gas release port; 3. Base; 4. Bracket; 5. Gas pressure sensor; 6. Test tube inversion rack; 7. Test tube; 8. Test tube fastening mechanism; 9. Fastening ring; 10. Connecting rod; 11. Threaded sleeve; 12. Stop rod; 13. Wedge block; 14. Threaded port; 15. Limit retaining ring. Specific implementation mode

[0023] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work shall fall within the protection scope of the present invention.

[0024] Embodiment 1: Please refer to Figures 1 to 5 , a device for constructing a cell model of lung blast injury, including a test tube inversion rack 6 and a gas release port 2. The gas release port 2 is arranged directly below the lower port of the test tube inversion rack 6. The gas release port 2 is connected to an air compressor through a gas release tube 1. Both the test tube inversion rack 6 and the gas release port 2 are fixedly installed on the bracket 4, and the bracket 4 is fixedly installed on the base 3. A gas pressure sensor 5 is arranged between the gas release port 2 and the lower port of the test tube inversion rack 6, and a test tube fastening mechanism 8 is arranged on the test tube inversion rack 6.

[0025] The test tube inversion rack 6 is arranged as a cylindrical structure that is vertically through. A horizontally inwardly extending limit retaining ring 15 is arranged on the lower port of the test tube inversion rack 6, and the limit retaining ring 15 is used to limit the port of the test tube.

[0026] The test tube fastening mechanism 8 includes a group of stop rods 12 arranged on the upper port of the test tube inversion rack 6 and vertically extending upward. A plurality of wedge blocks 13 are arranged equidistantly on the outer surface of the stop rod 12. A plurality of fastening rings 9 are sleeved outside the stop rod 12, and the plurality of fastening rings 9 are fixedly connected through a connecting rod 10. The fastening ring 9 is in clamping fit with the wedge block 13. When the fastening ring 9 is pressed down, the wedge block 13 is forced to retract inward, and the stop rod 12 clamps the test tube after retracting with the wedge block 13.

[0027] A threaded port 14 is arranged on the upper port of the test tube inversion rack 6. The bottom of the lowermost fastening ring 9 is rotatably connected with a threaded sleeve 11, and the threaded sleeve 11 is threadedly connected with the threaded port 14.

[0028] Anti-slip lines are arranged on the circumferential outer wall of the threaded sleeve 11.

[0029] A buffer cushion layer is connected to the inner surface of the stop rod 12.

[0030] Working principle of this embodiment:

[0031] First, prepare a lung cell suspension with a certain volume and concentration and place it in test tube 7. Seal the test tube with a sterile sealing film. Place the test tube containing test tube 7 in test tube inversion rack 6 and firmly fix it through test tube fastening mechanism 8 to ensure that the test tube does not shake or fall off during the gas impact process.

[0032] Then, start the air compressor and deliver high-pressure gas through gas release tube 1 to gas release port 2. The gas impacts test tube 7, causing damage to the lung cells inside the test tube. Gas pressure sensor 5 can monitor the gas pressure value in real time to ensure that the preset detonation pressure value is reached.

[0033] After the detonation shock, immediately sample the cells in the test tube and observe and analyze changes in cell morphology, function, gene expression, etc., so as to evaluate the impact of blast injury on the cells.

[0034] This embodiment can stably simulate the blast environment by precisely controlling the pressure of gas release, providing a reliable model for in-depth research on lung blast injury cells; the reliable test tube fixing mechanism effectively overcomes the influence of vibration on the results during the experiment of holding the test tube by hand.

[0035] The core of test tube fastening mechanism 8 in this embodiment lies in the clamping fit between stop rod 12 and fastening ring 9. A plurality of wedge-shaped blocks 13 provided on stop rod 12 are in an outward-expanded shape in the initial state. When a plurality of fastening rings 9 are connected through connecting rod 10 and pressed downward along stop rod 12, the inner wall of fastening ring 9 will squeeze the inclined surface of wedge-shaped block 13, forcing wedge-shaped block 13 to contract inward. Due to the special shape of wedge-shaped block 13, this squeezing will cause the distance between them to decrease, thereby enabling stop rod 12 as a whole to form a clamping effect on the test tube.

[0036] To ensure the stability and adjustability of the fastening effect, a threaded port 14 is designed at the upper port of test tube inversion rack 6 and is matched with the threaded sleeve 11 at the bottom of the lowermost fastening ring 9. By rotating threaded sleeve 11, the up and down movement of fastening ring 9 along stop rod 12 can be precisely controlled, thereby realizing the adjustment of the clamping degree of the test tube. Anti-slip threads are provided on the circumferential outer wall of threaded sleeve 11, increasing the friction during rotation, facilitating user operation and preventing hand slippage.

[0037] To reduce physical damage to the test tube and the cells inside, a buffer cushion layer is connected to the inner surface of stop rod 12. This layer of buffer material plays a buffering role when the test tube is clamped, capable of absorbing part of the impact brought by the clamping force and protecting the integrity of the test tube and its contents.

Claims

1. A device for constructing a lung blast injury cell model, comprising a test tube inversion rack (6) and a gas release port (2), characterized in that: The gas release port (2) is arranged directly below the lower port of the test tube inversion rack (6); the gas release port (2) is connected to an air compressor via a gas release pipe (1); the test tube inversion rack (6) and the gas release port (2) are both fixedly mounted on a bracket (4); the bracket (4) is fixedly mounted on a base (3); a gas pressure sensor (5) is arranged between the gas release port (2) and the lower port of the test tube inversion rack (6); and a test tube fastening mechanism (8) is arranged on the test tube inversion rack (6).

2. A lung blast injury cell model construction device according to claim 1, characterized in that: The test tube inversion rack (6) is configured as a cylindrical structure that is connected from top to bottom. A limit stop ring (15) that extends horizontally inward is provided on the lower port of the test tube inversion rack (6). The limit stop ring (15) is used to limit the port of the test tube.

3. A lung blast injury cell model construction device according to claim 1, characterized in that: The test tube fastening mechanism (8) comprises a group of baffles (12) arranged on the upper end of the test tube inversion rack (6) and extending vertically upwards, a plurality of wedge blocks (13) are arranged at equal distances on the outer surface of the baffles (12), a plurality of fastening rings (9) are sleeved on the outer side of the baffles (12), the plurality of fastening rings (9) are fixedly connected by connecting rods (10), the fastening rings (9) are engaged with the wedge blocks (13), when the fastening rings (9) are pressed downwards, the wedge blocks (13) are forced to retract, and the baffles (12) clamp the test tube after the wedge blocks (13) are retracted.

4. A lung blast injury cell model construction device according to claim 3, characterized in that: The upper end of the test tube inversion rack (6) is provided with a threaded opening (14), the bottom of the lowest fastening ring (9) is rotatably connected with a threaded sleeve (11), and the threaded sleeve (11) is threadedly connected to the threaded opening (14).

5. A lung blast injury cell model construction device according to claim 4, characterized in that: Anti-slip grooves are arranged on the circumferential outer wall of the threaded sleeve (11).

6. The device for constructing a lung blast injury cell model according to claim 3, characterized in that: A buffer layer is connected to the inner surface of the blocking rod (12).

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