Suspension cell culture bottle and cell culture equipment

Abstract

The utility model discloses a suspension cell culture bottle and cell culture equipment, and belongs to the field of cell culture. The suspension cell culture bottle comprises a container, a first telescopic mechanism, a second telescopic mechanism and a driving mechanism. The end, close to the opening, of the container is obliquely arranged towards the end, away from the opening, of the container in the axis direction away from the container. The first telescopic mechanism is telescopically arranged in the containing cavity in the axis direction of the container; the second telescopic mechanism is connected with the first telescopic mechanism and is telescopically arranged in the containing cavity in the direction perpendicular to the axis of the container, a plurality of clamping grooves matched with the end of the second telescopic mechanism are formed in the cavity wall of the containing cavity, and the multiple clamping grooves are formed in the inclination direction of the container at intervals; the driving mechanism is arranged outside the containing cavity and connected with the first telescopic mechanism. The suspension cell culture bottle provided by the utility model is simple and convenient to operate, cells do not need to be transferred into containers with different volumes, and the risk that the cells are polluted is effectively reduced.

Description

Technical Field

[0001] This application relates to the technical field of cell culture, and particularly to a suspension cell culture flask and a cell culture device. Background Art

[0002] Cell culture refers to a method of simulating the in-vivo environment in vitro to enable cells to survive, grow, reproduce, and maintain their main structures and functions. During cell culture, strict requirements are imposed on cell density, that is, the contact area between cells and the culture container is strictly controlled to provide a good culture environment for cells. Therefore, as cells grow and multiply, it is necessary to change the volume of the culture container multiple times to maintain a certain cell density.

[0003] However, currently, it is generally necessary to transfer cells to culture containers with different volumes multiple times to maintain a certain cell density, which is cumbersome to operate and has a high risk of cell contamination. Summary of the Utility Model

[0004] In view of this, the purpose of this application is to overcome the deficiencies in the prior art and provide a suspension cell culture flask to solve the technical problems in the prior art that it is cumbersome to operate and has a high risk of cell contamination due to transferring cells to culture containers with different volumes multiple times to maintain a certain cell density.

[0005] To solve the above technical problems, this application provides:

[0006] A suspension cell culture flask, comprising:

[0007] A container having a receiving cavity with an open end, and one end of the container near the opening is inclined towards the other end of the container away from the opening along a direction away from the axis of the container;

[0008] A first telescopic mechanism telescopically arranged in the receiving cavity along the axis of the container;

[0009] A second telescopic mechanism connected to the first telescopic mechanism and telescopically arranged in the receiving cavity along a direction perpendicular to the axis of the container. A plurality of clamping grooves adapted to the end of the second telescopic mechanism are formed in the cavity wall of the receiving cavity, and the plurality of clamping grooves are arranged at intervals along the inclined direction of the container;

[0010] A driving mechanism arranged outside the receiving cavity and connected to the first telescopic mechanism for driving the first telescopic mechanism to expand and contract along the axis of the container to drive the second telescopic mechanism to expand and contract along a direction perpendicular to the axis of the container.

[0011] In addition, the suspension cell culture flask according to this application may further have the following additional technical features:

[0012] In some embodiments of the present application, the first telescopic mechanism includes a rotating member and a first moving member. A first through hole is formed at one end of the container away from the opening. One end of the rotating member away from the opening passes through the first through hole and is connected to the driving mechanism. The first moving member is sleeved on the rotating member and is threadedly connected to the rotating member.

[0013] In some embodiments of the present application, the second telescopic mechanism includes a fixing member and a second moving member. The fixing member is disposed on the first moving member and is located in the circumferential direction of the rotating member. One end of the second moving member is movably disposed in the fixing member and is connected to the rotating member, and the other end movably passes through the fixing member and is engaged with the engaging groove.

[0014] In some embodiments of the present application, the second telescopic mechanism further includes an elastic member. The elastic member is disposed in the fixing member and abuts against the rotating member and the second moving member respectively.

[0015] In some embodiments of the present application, an installation groove is formed at one end of the second moving member away from the rotating member. A plurality of pH test papers are stacked in the installation groove. A second through hole communicating with the installation groove is formed in the circumferential wall at one end of the second moving member away from the rotating member, and the second through hole is located on the side of the second moving member close to the opening.

[0016] In some embodiments of the present application, a peeling portion is provided on the inner circumferential wall at one end of the fixing member away from the rotating member, and the peeling portion is used for peeling the pH test paper.

[0017] In some embodiments of the present application, the container is integrally formed of a transparent material.

[0018] In some embodiments of the present application, the suspension cell culture bottle further includes a colorimetric card, and a plurality of color regions corresponding to a plurality of pH values are provided on the colorimetric card.

[0019] In some embodiments of the present application, the suspension cell culture bottle further includes a cover body. The cover body covers the opening. An external thread is provided on the outer circumferential wall at one end of the container close to the opening, and an internal thread matching the external thread is provided on the cover body.

[0020] In a second aspect, an embodiment of the present application further provides a cell culture device, including the suspension cell culture bottle according to any one of the above embodiments.

[0021] Compared with the prior art, the beneficial effects of the present application are:

[0022] The present application provides a suspension cell culture flask. The container has a receiving cavity with one end open, and one end of the container near the opening is inclined towards the end of the container far from the opening along a direction away from the axis of the container, so that the cross-sectional area of the receiving cavity gradually increases along the direction away from the opening. A plurality of clamping grooves adapted to the end of the second telescopic mechanism are provided on the cavity wall of the receiving cavity, and the plurality of clamping grooves are arranged at intervals along the inclined direction of the container. In this way, when the end of the second telescopic mechanism cooperates with the clamping groove, the second telescopic mechanism can divide the receiving cavity into two receiving spaces. The receiving space between the second telescopic mechanism and the opening is used to receive cultured cells. Thus, when the second telescopic mechanism moves along the direction away from the opening, the receiving space for receiving cultured cells will gradually increase.

[0023] By providing a driving mechanism connected to the first telescopic mechanism outside the receiving cavity to drive the first telescopic mechanism to telescopically move along the axis of the container in the receiving cavity, thereby driving the second telescopic mechanism provided on the first telescopic mechanism to synchronously telescopically move along the axis of the container. At the same time, by connecting the second telescopic mechanism to the first telescopic mechanism, the second telescopic mechanism is driven to telescopically move along a direction perpendicular to the axis of the container under the transmission action of the first telescopic mechanism. In this way, when it is necessary to increase the receiving space for culturing cells as the cells grow, reproduce and expand, the driving mechanism is used to drive the first telescopic mechanism and drive the second telescopic mechanism to synchronously move and contract along the axis of the container, so that the second telescopic mechanism moves along the direction away from the opening. At the same time, under the transmission action of the first telescopic mechanism, the second telescopic mechanism is driven to telescopically move along a direction perpendicular to the axis of the container, so that the end of the second telescopic mechanism contracts, separates from the previous clamping groove, extends and cooperates with the next clamping groove, thereby gradually increasing the receiving space for receiving cultured cells, achieving the purpose of increasing the receiving space for culturing cells, maintaining a certain cell density, with simple and convenient operation, and without the need to transfer the cells into containers with different volumes, effectively reducing the risk of cell contamination. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following will briefly introduce the drawings required to be used in the embodiments. It should be understood that the following drawings only show some embodiments of the present application, and therefore should not be regarded as a limitation of the scope. For those of ordinary skill in the art, without creative efforts, other related drawings can also be obtained based on these drawings.

[0025] Figure 1 shows a cross-sectional schematic view of a suspension cell culture flask in some embodiments of the present application Figure 1 ;

[0026] Figure 2 shows Figure 1 an enlarged schematic view of the structure of part A in

[0027] Figure 3 Shows a schematic cross - section of a suspension cell culture flask in some embodiments of the present application Figure 2 ;

[0028] Figure 4 Shows a schematic cross - section of a suspension cell culture flask in some embodiments of the present application Figure 3 。

[0029] Description of main component symbols:

[0030] 100 - suspension cell culture flask; 110 - container; 111 - opening; 112 - accommodation cavity; 1121 - clamping groove; 113 - first through - hole; 120 - first telescopic mechanism; 121 - rotating part; 122 - first moving part; 130 - second telescopic mechanism; 131 - fixing part; 132 - second moving part; 1321 - mounting groove; 1322 - second through - hole; 140 - driving mechanism; 150 - pH test paper. Detailed implementation manners

[0031] The following details the embodiments of the present application. The examples of the embodiments are shown in the drawings, where the same or similar reference numerals represent the same or similar elements or elements with the same or similar functions throughout. The embodiments described below with reference to the drawings are exemplary and are only used to explain the present application and should not be construed as a limitation of the present application.

[0032] In the description of the present application, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as a limitation of the present application.

[0033] In addition, the terms "first" and "second" are only used for descriptive purposes and should not be construed as indicating or implying relative importance or implicitly indicating the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include one or more of such features. In the description of the present application, "a plurality" means two or more unless otherwise specifically defined.

[0034] In this application, unless otherwise clearly defined and limited, terms such as "installed", "connected", "joined", "fixed", etc. shall be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or integrated; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two components or the interaction relationship between two components. For those of ordinary skill in the art, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0035] In this application, unless otherwise clearly defined and limited, the first feature being "on" or "under" the second feature can be that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, the first feature being "above", "over" and "on top of" the second feature can be that the first feature is directly above or obliquely above the second feature, or merely indicates that the first feature has a higher horizontal height than the second feature. The first feature being "under", "beneath" and "underneath" the second feature can be that the first feature is directly below or obliquely below the second feature, or merely indicates that the first feature has a lower horizontal height than the second feature.

[0036] Embodiment 1

[0037] As Figure 1 , Figure 3 and Figure 4 shown, an embodiment of the present application provides a suspension cell culture flask 100. The suspension cell culture flask 100 includes a container 110, a first telescopic mechanism 120, a second telescopic mechanism 130, and a driving mechanism 140.

[0038] Among them, the container 110 has a receiving cavity 112 with an opening 111 at one end, and one end of the container 110 near the opening 111 is inclined towards the end of the container 110 away from the opening 111 in a direction away from the axis of the container 110.

[0039] The first telescopic mechanism 120 is telescopically arranged in the receiving cavity 112 along the axis of the container 110. The second telescopic mechanism 130 is connected to the first telescopic mechanism 120 and is telescopically arranged in the receiving cavity 112 along a direction perpendicular to the axis of the container 110. A plurality of clamping grooves 1121 adapted to the end of the second telescopic mechanism 130 are formed on the cavity wall of the receiving cavity 112, and the plurality of clamping grooves 1121 are arranged at intervals along the inclined direction of the container 110.

[0040] The driving mechanism 140 is disposed outside the accommodating cavity 112 and connected to the first telescopic mechanism 120, and is configured to drive the first telescopic mechanism 120 to telescopically move along the axis direction of the container 110, so as to drive the second telescopic mechanism 130 to telescopically move along a direction perpendicular to the axis direction of the container 110.

[0041] For the suspension cell culture flask 100 provided by an embodiment of the present application, the container 110 has an accommodating cavity 112 with an opening 111 at one end, and one end of the container 110 near the opening 111 is inclined towards the end of the container 110 away from the opening 111 along a direction away from the axis direction of the container 110, so that the cross-sectional area of the accommodating cavity 112 gradually increases along the direction away from the opening 111. A plurality of clamping grooves 1121 adapted to the end of the second telescopic mechanism 130 are formed on the cavity wall of the accommodating cavity 112, and the plurality of clamping grooves 1121 are arranged at intervals along the inclined direction of the container 110. In this way, when the end of the second telescopic mechanism 130 cooperates with the clamping groove 1121, the second telescopic mechanism 130 can divide the accommodating cavity 112 into two accommodating spaces. The accommodating space between the second telescopic mechanism 130 and the opening 111 is used to accommodate the cultured cells. Thus, when the second telescopic mechanism 130 moves along the direction away from the opening 111, the accommodating space for accommodating the cultured cells will gradually increase.

[0042] By disposing a driving mechanism 140 connected to the first telescopic mechanism 120 outside the accommodating cavity 112 to drive the first telescopic mechanism 120 to telescopically move along the axis direction of the container 110 within the accommodating cavity 112, thereby driving the second telescopic mechanism 130 disposed on the first telescopic mechanism 120 to synchronously telescopically move along the axis direction of the container 110. At the same time, by connecting the second telescopic mechanism 130 to the first telescopic mechanism 120, the second telescopic mechanism 130 is driven to telescopically move along a direction perpendicular to the axis direction of the container 110 under the transmission action of the first telescopic mechanism 120.

[0043] In this way, when it is necessary to increase the accommodating space for culturing cells as the cells grow, multiply and expand, the driving mechanism 140 drives the first telescopic mechanism 120 and drives the second telescopic mechanism 130 to synchronously move and contract along the axis direction of the container 110, so that the second telescopic mechanism 130 moves along the direction away from the opening 111. At the same time, under the transmission action of the first telescopic mechanism 120, the second telescopic mechanism 130 is driven to telescopically move along a direction perpendicular to the axis direction of the container 110, so that the end of the second telescopic mechanism 130 contracts, separates from the previous clamping groove 1121, extends and cooperates with the next clamping groove 1121, thereby gradually increasing the accommodating space for accommodating the cultured cells, achieving the purpose of increasing the accommodating space for culturing cells, maintaining a certain cell density, with simple and convenient operation, and without the need to transfer the cells into containers with different volumes, effectively reducing the risk of cell contamination.

[0044] As Figure 1 , Figure 3 and Figure 4 shown, in an embodiment of the present application, the first telescopic mechanism 120 includes a rotating member 121 and a first moving member 122. One end of the container 110 away from the opening 111 is provided with a first through hole 113. One end of the rotating member 121 away from the opening 111 passes through the first through hole 113 and is connected to the driving mechanism 140. The first moving member 122 is sleeved on the rotating member 121 and is threadedly connected to the rotating member 121.

[0045] In this embodiment, by passing one end of the rotating member 121 away from the opening 111 through the first through hole 113 of the container 110 and connecting it to the driving mechanism 140, and sleeving the first moving member 122 on the rotating member 121 and threadedly connecting it to the rotating member 121, when the driving mechanism 140 drives the rotating member 121 to rotate, the first moving member 122 can move along the axial direction of the rotating member 121, realizing the function of the first telescopic mechanism 120 telescoping along the axial direction of the container 110.

[0046] As Figure 1 , Figure 3 and Figure 4 shown, in the above embodiment of the present application, the second telescopic mechanism 130 includes a fixing member 131 and a second moving member 132. The fixing member 131 is disposed on the first moving member 122 and is located in the circumferential direction of the rotating member 121. One end of the second moving member 132 is movably disposed within the fixing member 131 and is connected to the rotating member 121, and the other end movably passes through the fixing member 131 and is snap-connected to the snap groove 1121.

[0047] In this embodiment, by disposing the fixing member 131 on the first moving member 122 and in the circumferential direction of the rotating member 121 to move synchronously along the axial direction of the rotating member 121 with the first moving member 122, the fixing member 131 can move along the axial direction of the container 110. One end of the second moving member 132 is disposed within the fixing member 131, so that the second moving member 132 can move synchronously along the axial direction of the container 110.

[0048] One end of the second moving member 132 is movably arranged in the fixing member 131 and connected to the rotating member 121, and the other end of the second moving member 132 movably passes through the fixing member 131 and is clamped with the clamping groove 1121. In this way, when it is necessary to increase the accommodation space for culturing cells as the cells grow and multiply, the driving mechanism 140 drives the first telescopic mechanism 120 and drives the second telescopic mechanism 130 to synchronously move and contract along the axial direction of the container 110, so that the second telescopic mechanism 130 moves away from the opening 111. At the same time, under the driving action of the rotating member 121, the second moving member 132 is driven to expand and contract along the direction perpendicular to the axis of the container 110, so that the other end of the second moving member 132 contracts and separates from the previous clamping groove 1121 and then expands and cooperates with the next clamping groove 1121, thereby gradually increasing the accommodation space for accommodating the cultured cells.

[0049] In the above embodiment of the present application, the second telescopic mechanism 130 further includes an elastic member, and the elastic member is arranged in the fixing member 131 and abuts against the rotating member 121 and the second moving member 132 respectively.

[0050] In this embodiment, by arranging the elastic member in the fixing member 131 and abutting against the rotating member 121 and the second moving member 132 respectively, when it is necessary to increase the accommodation space for culturing cells as the cells grow and multiply, under the driving action of the rotating member 121, the second moving member 132 is driven to contract along the direction perpendicular to the axis of the container 110, so that the other end of the second moving member 132 contracts and separates from the previous clamping groove 1121. After separation, under the elastic force of the elastic member, the second moving member 132 is driven to expand along the direction perpendicular to the axis of the container 110 and cooperate with the next clamping groove 1121, thereby gradually increasing the accommodation space for accommodating the cultured cells.

[0051] As Figure 1 、 Figure 2 and Figure 3 As shown in the above embodiment of the present application, an installation groove 1321 is formed at one end of the second moving member 132 away from the rotating member 121, and multiple pH test papers 150 are stacked in the installation groove 1321. A second through hole 1322 communicating with the installation groove 1321 is formed in the circumferential wall of one end of the second moving member 132 away from the rotating member 121, and the second through hole 1322 is located on the side of the second moving member 132 close to the opening 111.

[0052] In this embodiment, an installation groove 1321 is formed at one end of the second moving member 132 away from the rotating member 121. A plurality of pH test papers 150 are stacked inside the installation groove 1321. A second through hole 1322 communicating with the installation groove 1321 is formed in the circumferential wall of the second moving member 132 at the end away from the rotating member 121, and the second through hole 1322 is located on the side of the second moving member 132 close to the opening 111. In this way, when the accommodation space for culturing cells needs to be increased as the cells grow, reproduce, and expand, the second moving member 132 is driven by the rotating member 121 to expand and contract in a direction perpendicular to the axis of the container 110, so that the other end of the second moving member 132 contracts and separates from the previous clamping groove 1121. After separation, the cell liquid in the accommodation space can be dropped onto the pH test paper 150 through the second through hole 1322 to detect the pH value of the cell liquid, thereby judging the cell density and growth condition.

[0053] In the above embodiment of the present application, a peeling portion is provided on the inner circumferential wall of the fixing member 131 at the end away from the rotating member 121, and the peeling portion is used for peeling the pH test paper 150.

[0054] In this embodiment, by providing a peeling portion on the inner circumferential wall of the fixing member 131 at the end away from the rotating member 121, when the accommodation space for culturing cells needs to be increased as the cells grow, reproduce, and expand, the second moving member 132 is driven by the rotating member 121 to expand and contract in a direction perpendicular to the axis of the container 110, so that the other end of the second moving member 132 separates from the previous clamping groove 1121 and contracts into the fixing member 131. Thus, the peeling portion can automatically peel the used pH test paper 150, which is convenient for the cell liquid to be dropped onto the unused pH test paper 150 through the second through hole 1322 to detect the pH value of the cell liquid when the accommodation space is increased next time.

[0055] In the above embodiment of the present application, the container 110 is integrally formed of a transparent material.

[0056] In this embodiment, the container 110 is integrally formed of a transparent material, so as to directly observe the color of the pH test paper 150 through the container 110, thereby facilitating the judgment of the cell density and growth condition.

[0057] In the above embodiment of the present application, the suspension cell culture flask 100 further includes a color comparison card, and a plurality of color regions corresponding to a plurality of pH values are provided on the color comparison card.

[0058] In this embodiment, by providing a plurality of color regions corresponding to a plurality of pH values on the color comparison card, it is convenient to compare the color shown after the pH test strip 150 detects the cell fluid with the plurality of color regions of the color comparison card to obtain the pH value of the cell fluid, thereby facilitating the judgment of cell density and growth conditions.

[0059] In any of the above embodiments of the present application, the suspension cell culture flask 100 further includes a lid, the lid is covered on the opening 111, an external thread is provided on the outer circumferential wall of the container 110 near one end of the opening 111, and the lid is provided with an internal thread that cooperates with the external thread.

[0060] In this embodiment, by covering the lid on the opening 111, the sealing function of the accommodation space for culturing cells is realized, effectively reducing the risk of cell contamination. At the same time, by providing an external thread on the outer circumferential wall of the container 110 near one end of the opening 111 and providing an internal thread that cooperates with the external thread on the lid, the lid is detachably connected to the container 110 near one end of the opening 111, so as to facilitate opening the lid to supplement liquid into the accommodation space or transfer the cell fluid after culturing is completed.

[0061] Embodiment 2

[0062] The embodiment of the present application further provides a cell culture device, including the suspension cell culture flask 100 described in the above embodiments.

[0063] This cell culture device has the suspension cell culture flask 100 in any of the above embodiments, and thus has all the beneficial effects of the suspension cell culture flask 100, which will not be elaborated here one by one.

[0064] In the description of this specification, the description with reference to terms such as "one embodiment", "some embodiments", "example", "specific example", or "some examples" means that the specific features, structures, materials, or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present application. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described can be combined in a suitable manner in any one or more embodiments or examples. In addition, without conflict, those skilled in the art can combine and combine the different embodiments or examples described in this specification and the features of different embodiments or examples.

[0065] Although the embodiments of the present application have been shown and described above, it can be understood that the above embodiments are exemplary and should not be construed as limiting the present application. Those of ordinary skill in the art can make changes, modifications, substitutions, and variations to the above embodiments within the scope of the present application.

Claims

1. A suspension cell culture flask, characterized in that: include: A container having a receiving cavity with an opening at one end, wherein the end of the container close to the opening is inclined along an axis away from the container toward the end of the container away from the opening; A first telescopic mechanism is telescopically disposed in the accommodating cavity along the axial direction of the container; A second telescopic mechanism is connected to the first telescopic mechanism and is telescopically arranged in the accommodating cavity along a direction perpendicular to the axis of the container. The cavity wall of the accommodating cavity is provided with a plurality of snap-in grooves adapted to the ends of the second telescopic mechanism. The plurality of snap-in grooves are arranged at intervals along the tilting direction of the container. The driving mechanism is arranged outside the accommodating cavity and connected to the first telescopic mechanism, and is used to drive the first telescopic mechanism to telescope along the axial direction of the container, so as to drive the second telescopic mechanism to telescope along the axial direction perpendicular to the container.

2. The suspension cell culture bottle according to claim 1, characterized in that: The first telescopic mechanism includes a rotating member and a first movable member. A first through hole is provided at one end of the container away from the opening. One end of the rotating member away from the opening is passed through the first through hole and connected to the driving mechanism. The first movable member is sleeved on the rotating member and is threadedly connected to the rotating member.

3. The suspension cell culture bottle according to claim 2, characterized in that: The second telescopic mechanism includes a fixed part and a second movable part. The fixed part is arranged on the first movable part and is located in the circumferential direction of the rotating part. One end of the second movable part is movably arranged in the fixed part and connected to the rotating part, and the other end is movably passed through the fixed part and is clamped with the clamping groove.

4. The suspension cell culture bottle according to claim 3, characterized in that: The second telescopic mechanism further includes an elastic member, which is disposed in the fixing member and abuts against the rotating member and the second moving member respectively.

5. The suspension cell culture bottle according to claim 3, characterized in that: An installation groove is provided at one end of the second movable member away from the rotating member, and a plurality of pH test strips are stacked in the installation groove. A second through hole connected to the installation groove is provided on the circumferential wall of the end of the second movable member away from the rotating member, and the second through hole is located on a side of the second movable member close to the opening.

6. The suspension cell culture bottle according to claim 5, characterized in that: A peeling portion is provided on the inner circumferential wall of one end of the fixing member away from the rotating member, and the peeling portion is used to peel off the pH test paper.

7. The suspension cell culture bottle according to claim 5, characterized in that: The container is made of transparent material and is integrally formed.

8. The suspension cell culture bottle according to claim 7, characterized in that: The suspension cell culture bottle also includes a colorimetric card, on which a plurality of color regions corresponding to a plurality of pH values ​​are arranged.

9. The suspension cell culture flask according to any one of claims 1 to 8, characterized in that: The suspension cell culture bottle also includes a cover body, which is arranged on the opening. An outer circumferential wall of one end of the container close to the opening is provided with an external thread, and the cover body is provided with an internal thread matching the external thread.

10. A cell culture device, characterized in that: The suspension cell culture flask comprises the suspension cell culture flask according to any one of claims 1 to 9.

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