Flow path selection valve and liquid path system

Abstract

The utility model discloses a flow path selection valve and a liquid path system. The flow path selection valve is configured to switch between a first valve position and a second valve position, the flow path selection valve is provided with a common port, a plurality of first ports, a plurality of second ports, a first communication groove and a second communication groove, and when the flow path selection valve is in the first valve position, the first communication groove is communicated with the second communication groove. When the flow path selection valve is in the first valve position, the first ports communicate with the common port through first communicating grooves, and when the flow path selection valve is in the second valve position, the first ports communicate with the second ports through second communicating grooves in a one-to-one correspondence mode. Therefore, the control of liquid flow path switching can be realized, the number of the three-way valves in the liquid path system is reduced, and the structure of the liquid path system is simplified, so that the cost of the liquid path system is reduced, and the sequencing cost is further reduced. Besides, different liquid inlet modes can be selected, so that the liquid loading flexibility is improved, and the sequencing quality and the sequencing efficiency are improved.

Description

Technical Field

[0001] The utility model relates to the field of gene sequencing, in particular to a flow path selection valve and a liquid path system. Background Art

[0002] Gene sequencing technology refers to the technical means of obtaining the DNA or RNA base sequence through detection. Currently, the dominant sequencing technology is high-throughput sequencing technology. In the sequencing platform that realizes high-throughput sequencing based on sequencing by synthesis, the general process of gene sequencing includes fixing the nucleic acid sample to be tested on a flow cell in a way such as hybridization; then using PCR amplification to form nucleic acid molecule clusters of the nucleic acid sample to be tested; then adding sequencing reagents such as bases with fluorescent groups, polymerase, and primers into the flow cell through a liquid path system, and through the principle of base complementary pairing, making the bases with fluorescent groups bind to the bases on the nucleic acid sample to be tested; finally, exciting the fluorescent groups to generate fluorescence through an optical imaging system and collecting the fluorescence to form an image, and identifying the bases through the image, so as to realize the determination of the base sequence of the nucleic acid sample to be tested.

[0003] In the related art, the structure of the liquid path system is complex and the liquid inlet mode is single, so that the speed of the sample to be tested and the reagent entering the flow cell is slow, the liquid inlet time is long, and the liquid inlet efficiency and sequencing efficiency are reduced. Summary of the Utility Model

[0004] The utility model provides a flow path selection valve and a liquid path system.

[0005] An embodiment of the present application provides a flow path selection valve, which is configured to switch between a first valve position and a second valve position. The flow path selection valve is provided with a common port, a plurality of first ports, a plurality of second ports, a first communication groove and a second communication groove. When the flow path selection valve is in the first valve position, the plurality of first ports are communicated with the common port through the first communication groove. When the flow path selection valve is in the second valve position, the first ports are communicated with the second ports in one-to-one correspondence through the second communication groove.

[0006] In this way, through the flow path selection valve, the first port can be selectively communicated with the common port and the second port, realizing the control of the switching of the liquid flow path, reducing the number of three-way valves in the liquid path system, simplifying the structure of the liquid path system, thereby reducing the cost of the liquid path system, and further reducing the sequencing cost. In addition, the liquid can flow from the common port to the plurality of first ports through the first communication groove, or can flow from the plurality of first ports to the corresponding second ports through the plurality of second communication grooves. Thus, different liquid inlet modes can be selected, improving the flexibility of liquid loading, and thereby improving the sequencing quality and sequencing efficiency.

[0007] In some embodiments, the first communication groove includes a main communication groove and a plurality of branch communication grooves. The plurality of branch communication grooves are all communicated with the main communication groove, and the plurality of branch communication grooves are arranged in one-to-one correspondence with a plurality of first ports; the plurality of first ports and the common port are communicated through the main communication groove and the plurality of branch communication grooves.

[0008] In some embodiments, the first communication groove includes a main communication groove and multiple groups of branch communication grooves. The multiple groups of branch communication grooves are communicated with the main communication groove, and the multiple groups of branch communication grooves include a plurality of branch communication grooves. The plurality of branch communication grooves are arranged in one-to-one correspondence with a plurality of first ports, and the plurality of first ports and the common port are communicated through the main communication groove and the plurality of branch communication grooves.

[0009] In some embodiments, the multiple groups of branch communication grooves include a first group of branch communication grooves and a second group of branch communication grooves; the first group of branch communication grooves is communicated with the main communication groove through a first flow path; the second group of branch communication grooves is communicated with the main communication groove through a second flow path.

[0010] In some embodiments, a liquid inlet end is arranged on the main communication groove, and a liquid outlet end is arranged on each branch communication groove. The liquid inlet end is communicated with the common port, and the liquid outlet end is communicated with the first port.

[0011] In some embodiments, there are multiple second communication grooves. Each second communication groove has two ends. One end of each second communication groove is communicated with a first port, and the other end is communicated with a second port.

[0012] In some embodiments, the flow path selection valve includes a stator and a rotor arranged opposite to the stator. The stator is provided with a common port, a plurality of first ports and a plurality of second ports, and the rotor is provided with a first communication groove and a second communication groove.

[0013] In some embodiments, the number of common ports is multiple. When the flow path selection valve is in the first valve position, each common port is communicated with the plurality of first ports through the first communication groove.

[0014] An embodiment of the present application provides a liquid path system, including a flow cell, a flow path selection valve and a power assembly. The flow cell includes a plurality of fluid channels for carrying a sample to be tested and / or a reagent; the flow path selection valve is arranged upstream of the flow cell, and the plurality of second ports of the flow path selection valve are communicated with the plurality of fluid channels and arranged in one-to-one correspondence; the power assembly is used to provide power to enable the sample to be tested and / or the reagent to enter the fluid channel through the flow path selection valve.

[0015] In some embodiments, the liquid path system includes a first memory, and the first memory is communicated with the common port of the flow path selection valve. The first memory is used to store the sample to be tested and / or the reagent.

[0016] In some embodiments, the power assembly includes a first pump group and a second pump group. The first pump group is disposed upstream of the flow cell and communicates with the first port of the flow path selection valve. The first pump group is selectively connected to the flow path selection valve and the first memory. The first pump group is configured to suck the sample to be tested and / or the reagent in a negative pressure driving manner, and make the sample to be tested and / or the reagent enter the fluid channel in a positive pressure driving manner. The second pump group is disposed downstream of the flow cell, and the second pump group is configured to make the sample to be tested and / or the reagent sucked by the first pump group enter the fluid channel in a negative pressure driving manner.

[0017] In some embodiments, the liquid path system includes a plurality of first buffer regions. Each first buffer region communicates with a first port and the first pump group. When the flow path selection valve is in the first valve position, the first pump group is configured to suck a single sample to be tested and / or the reagent from the first memory in a negative pressure driving manner, and pump the single sample to be tested and / or the reagent to each first buffer region.

[0018] In some embodiments, when the flow path selection valve is in the second valve position, the first pump group is configured to pump the sample to be tested and / or the reagent in each first buffer region to the corresponding fluid channel in a positive pressure driving manner; and / or, the second pump group is configured to pump the sample to be tested and / or the reagent in each first buffer region to the corresponding fluid channel in a negative pressure driving manner.

[0019] In some embodiments, the liquid path system includes a plurality of first switching valves. The first switching valves are disposed between the first pump group and the flow path selection valve and are used to control the on / off of the connection between the first pump group and the flow path selection valve.

[0020] In some embodiments, the liquid path system includes a plurality of second buffer regions and a plurality of second memories. Each second buffer region communicates with the first pump group and a second switching valve. The first pump group is configured to suck a plurality of samples to be tested from the second memory in a negative pressure driving manner, and pump each sample to be tested to a corresponding second buffer region.

[0021] In some embodiments, when the flow path selection valve is in the second valve position, the first pump group is configured to pump the sample to be tested in each second buffer region to the corresponding fluid channel in a positive pressure driving manner; and / or, the second pump group is configured to pump the sample to be tested in each second buffer region to the corresponding fluid channel in a negative pressure driving manner.

[0022] In some embodiments, the liquid path system includes a plurality of second switching valves. Each second switching valve is disposed between the corresponding second memory and the second buffer region and is used to control the on / off of the connection between the second memory and the second buffer region.

[0023] In some embodiments, the liquid path system includes a rotary valve that communicates with a common port of the first memory and the flow path selection valve.

[0024] Additional aspects and advantages of the present utility model will be given in part in the following description, become apparent in part from the following description, or be learned through the practice of the present utility model. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] The above and / or additional aspects and advantages of the present utility model will become apparent and be readily understood from the description of the embodiments in conjunction with the following drawings, wherein:

[0026] Figure 1 is a schematic structural diagram of the flow path selection valve according to an embodiment of the present utility model;

[0027] Figure 2 is a schematic structural diagram of the flow path selection valve according to an embodiment of the present utility model;

[0028] Figure 3 is a schematic structural diagram of the flow path selection valve according to an embodiment of the present utility model;

[0029] Figure 4 is a schematic structural diagram of the liquid path system according to an embodiment of the present utility model.

[0030] DESCRIPTION OF THE REFERENCE NUMERALS: 100, liquid path system; 10, flow path selection valve; 11, common port; 12, first port; 13, second port; 14, first communication groove; 141, main communication groove; 142, branch communication groove; 143, first flow path; 144, second flow path; 15, second communication groove; 16, stator; 17, rotor; 20, flow cell; 21, fluid channel; 30, power assembly; 31, first pump group; 32, second pump group; 33, first single pump; 34, second single pump; 40, first memory; 41, second memory; 50, first buffer area; 51, second buffer area; 60, first switching valve; 61, second switching valve; 70, rotary valve; 80, waste liquid bottle. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0031] The embodiments of the present utility model will be described in detail below. Examples of the embodiments are shown in the drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the drawings are exemplary only for explaining the present utility model and should not be construed as limiting the present utility model.

[0032] In the description of the present utility model, it should be understood that the orientation or positional relationship indicated by the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc. is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation to the present utility model. In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include one or more of the said features. In the description of the present utility model, "a plurality of" means two or more, unless otherwise specifically defined.

[0033] In the description of the present utility model, it should be noted that unless otherwise clearly specified and limited, the terms "installed", "connected" and "coupled" should be understood in a broad sense. For example, it may be a fixed connection, a detachable connection or an integral connection; it may be a mechanical connection, an electrical connection or a connection capable of mutual communication; it may be directly connected or indirectly connected through an intermediate medium, and it may be the communication inside two elements or the interaction relationship between two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.

[0034] In the present utility model, unless otherwise clearly specified and limited, the first feature being "on" or "under" the second feature may include the direct contact between the first and second features, or may include the situation where the first and second features are not in direct contact but in contact through other features therebetween. Moreover, the first feature being "above", "over" and "on top of" the second feature includes that the first feature is directly above and obliquely above the second feature, or merely means that the horizontal height of the first feature is higher than that of the second feature. The first feature being "under", "beneath" and "underneath" the second feature includes that the first feature is directly below and obliquely below the second feature, or merely means that the horizontal height of the first feature is lower than that of the second feature.

[0035] The following disclosure provides many different embodiments or examples for implementing different structures of the present utility model. To simplify the disclosure of the present utility model, components and settings of specific examples are described below. Of course, they are merely examples and are not intended to limit the present utility model. In addition, the present utility model may repeat reference numerals and / or reference letters in different examples. Such repetition is for the purpose of simplification and clarity and does not itself indicate the relationship between the various embodiments and / or settings discussed. In addition, the present utility model provides examples of various specific processes and materials, but those of ordinary skill in the art may be aware of the application of other processes and / or the use of other materials.

[0036] As used herein, "sequencing" refers to nucleic acid sequence determination, the same as "nucleic acid sequencing" or "gene sequencing", which means determining the base sequence of the primary structure of a nucleic acid molecule. It can be achieved by methods such as sequencing by synthesis (SBS), sequencing by ligation (SBL), or sequencing by hybridization (SBH). The so-called sequencing by synthesis includes not only the commonly understood SBS (such as the typical ILLUMINA / Solexa technology) that uses polymerase to catalyze nucleotide incorporation into the sample to be sequenced (synthesis reaction) and detects the corresponding reaction signals to identify the types of incorporated nucleotides, but also sequencing similar to SBS that uses polymerase or non-polymerase to controllably introduce or ligate nucleotides to the sample to be sequenced and directly or indirectly detects the corresponding signals to determine the types of ligated nucleotides.

[0037] Sequencing may include DNA sequencing and / or RNA sequencing. It includes long-fragment sequencing and / or short-fragment sequencing. The so-called long fragments and short fragments are relative. For example, nucleic acid molecules longer than 1Kb, 2Kb, 5Kb, or 10Kb can be called long fragments, and those shorter than 1Kb or 800bp can be called short fragments; it may include paired-end sequencing, single-end sequencing, and / or mate-pair sequencing, etc. The so-called paired-end sequencing or mate-pair sequencing can refer to the readout of any two non-overlapping parts of the same nucleic acid molecule.

[0038] Sequencing can be carried out through a sequencing platform. According to the embodiments of the present application, the selectable sequencing platforms include but are not limited to the Hiseq, Miseq, Nextseq, and Novaseq sequencing platforms of Illumina, the Ion Torrent platform of Thermo Fisher / Life Technologies, the BGISEQ and MGISEQ / DNBSEQ platforms of BGI, and single-molecule sequencing platforms; the sequencing method can be selected as single-end sequencing, paired-end sequencing, or the sequencing methods supported by the selected automated sequencing platform, etc.

[0039] Sequencing generally includes: library preparation, PCR amplification (not necessary), sequencing, and data analysis. The sequences read out by sequencing are called sequencing sequences, also known as reads.

[0040] In some examples, sequencing by synthesis is used for multiple rounds of sequencing to obtain sequencing sequences or reads. For example, the sample to be tested is contacted with polymerase and modified nucleotides and placed under conditions suitable for polymerization reaction, and the modified nucleotides are controllably incorporated into the sample to be tested or single-base extension is controllably achieved, and the corresponding reaction signals are detected. Based on this signal, the type of nucleotide incorporated into the sample to be tested in this reaction is determined. In this way, multiple controllable single-base extensions and corresponding signal detections are performed, so as to detect the types of nucleotides or bases incorporated into the sample to be tested in multiple or multiple rounds of reactions according to the reaction signal information, in order to read out a part of the sequence of the sample to be tested.

[0041] The sample to be tested, also known as the template to be tested or template, can be an unamplified single molecule, or a molecular cluster or long chain containing multiple identical polynucleotide molecules after amplification, such as the clone clusters or DNA nanoballs (DNBs) formed by bridge amplification or rolling circle amplification used in mainstream sequencing platforms. The sample to be tested can be presented in the form of single-stranded, double-stranded, and / or a complex hybridized with probes or primers.

[0042] The corresponding reaction signals can be, for example, fluorescence signals, or can be converted into image data formed by collecting these fluorescence signals. Thus, these image data are processed and analyzed to detect the nucleotides incorporated into the sample to be tested in each or each round of reaction, so as to determine a part of the base sequence of the sample to be tested.

[0043] Specifically, in some examples, sequencing is achieved based on surface fluorescence imaging detection. The sample to be tested is connected to a solid surface. For example, the modified nucleotides make the nucleotides carry or can bind to fluorescent labels, and have excisable inhibitory groups that can prevent other nucleotides from polymerizing and connecting to the next position of the sample to be tested (such modified nucleotides are also called reversible terminators). And after each polymerization reaction or single-base extension reaction is completed, fluorescence labels are excited to emit light, and these emitted light signals are collected to obtain an image of the sample to be tested where single-base extension reactions occur at a specified surface position; then, the inhibitory groups and fluorescent labels are removed, etc. to perform the next or the next round of polymerization reaction and signal collection (taking pictures). In this way, multiple or multiple rounds of polymerization-photographing-excision are repeated to obtain image set information related to the nucleotides connected to the sample to be tested in each single-base extension reaction.

[0044] Understandably, when a sample to be tested at a specified surface position undergoes a polymerization reaction and emits fluorescence, it generally appears as bright spots or blotches (spots) with a signal intensity higher than the background at the corresponding position in the image collected in this round of reaction. Therefore, based on the information of these image sets including the bright spots corresponding to specific chemical features (samples to be tested undergoing polymerization reactions) therein, it can be determined whether the samples to be tested at the specified positions have undergone polymerization reactions. Combining the preset corresponding relationship between distinguishable fluorescence emission signals and nucleotide types, the types of nucleotides that have undergone polymerization and been ligated to the samples to be tested can be detected, and thus at least a part of the sequence of the samples to be tested can be determined to obtain the so-called read segments.

[0045] It should be noted that the so-called nucleotides include ribonucleic acid or deoxyribonucleic acid, including natural nucleotides or their derivatives or modified forms (also referred to as modified nucleotides or modified nucleotides, etc.). In this article, sometimes the bases contained in nucleotides are also used to refer to such nucleotides, which can be clearly understood by those skilled in the art based on common knowledge and / or context.

[0046] Please refer to Figure 1 and Figure 2 , an embodiment of the present application provides a flow path selection valve 10, which is configured to switch between a first valve position and a second valve position. The flow path selection valve 10 is provided with a common port 11, a plurality of first ports 12, a plurality of second ports 13, a first communication groove 14 and a second communication groove 15. When the flow path selection valve 10 is in the first valve position, the plurality of first ports 12 communicate with the common port 11 through the first communication groove 14. When the flow path selection valve 10 is in the second valve position, the first ports 12 communicate with the second ports 13 in a one-to-one correspondence through the second communication groove 15.

[0047] In this way, through the flow path selection valve 10, the first ports 12 can be selectively communicated with the common port 11 and the second ports 13, realizing the control of the switching of the liquid flow path, reducing the number of three-way valves in the liquid path system 100, simplifying the structure of the liquid path system 100, thereby reducing the cost of the liquid path system 100, and further reducing the sequencing cost. In addition, the liquid can flow from the common port 11 to the plurality of first ports 12 through the first communication groove 14, or can flow from the plurality of first ports 12 to the corresponding second ports 13 through the plurality of second communication grooves 15. Thus, different liquid inlet modes can be selected, improving the flexibility of liquid loading, and thereby improving the sequencing quality and sequencing efficiency.

[0048] Specifically, the common port 11 can serve as the liquid inlet of the flow path selection valve 10 or the liquid outlet. In other words, the liquid can enter or flow out of the flow path selection valve 10 from the common port 11, thereby achieving flow splitting or flow merging. In this application, the common port 11 serves as the liquid inlet of the flow path selection valve 10, and the liquid can enter the flow path selection valve 10 from the common port 11. The common port 11 can be in a regular shape such as a circle or a polygon, or an irregular shape. The shapes of each common port 11 can be the same or different. In the embodiments of this application, in order to facilitate the formation, manufacture, and / or connection with common pipelines of the common port 11, the common port 11 is circular.

[0049] The first port 12 and the second port 13 can serve as the liquid inlet of the flow path selection valve 10 or the liquid outlet. The number of the first ports 12 can be the same as that of the second ports 13. For example, the number of both the first ports 12 and the second ports 13 is three, four, five, or more. The first port 12 and the second port 13 can be in a regular shape such as a circle or a polygon, or an irregular shape. The shapes of each first port 12 and second port 13 can be the same or different. In the embodiments of this application, in order to facilitate the formation, manufacture, and / or connection with common pipelines of the first port 12 and the second port 13, the first port 12 and the second port 13 are circular, and the cross-sections of the first communication groove 14 and the second communication groove 15 are also circular. For the convenience of manufacture, the size of the first port 12 can be the same as that of the second port 13.

[0050] In some embodiments, the first communication groove 14 includes a main communication groove 141 and a plurality of branch communication grooves 142. The plurality of branch communication grooves 142 are all communicated with the main communication groove 141, and the plurality of branch communication grooves 142 are arranged in one-to-one correspondence with the plurality of first ports 12; the plurality of first ports 12 and the common port 11 are communicated through the main communication groove 141 and the plurality of branch communication grooves 142.

[0051] In this way, the liquid can enter from the common port 11 and flow out from the plurality of first ports 12 through the main communication groove 141 and the plurality of branch communication grooves 142, thereby realizing the splitting of a single liquid.

[0052] Specifically, the number of the main communication grooves 141 is one, and the number of the branch communication grooves 142 can be three, four, five, six, or more. For example, the common port 11 is communicated with the main communication groove 141, the number of the first ports 12 is four, and the number of the branch communication grooves 142 is four. One ends of the four branch communication grooves 142 are communicated with the four first ports 12 in one-to-one correspondence, and the other ends are communicated with the main communication groove 141.

[0053] The main communication groove 141 and the branch communication grooves 142 can be in a bent shape, a curved shape, etc. The specific shapes of the main communication groove 141 and the branch communication grooves 142 are not limited herein. In one embodiment, both the main communication groove 141 and the branch communication grooves 142 are linear grooves, so that the distance from the common port 11 to the first port 12 is relatively short, the liquid inlet time can be shortened, and thus the sequencing efficiency can be improved.

[0054] Please refer to Figure 1 , in some embodiments, the first communication groove 14 includes a main communication groove 141 and multiple groups of branch communication grooves 142. The multiple groups of branch communication grooves 142 are communicated with the main communication groove 141, and the multiple groups of branch communication grooves 142 include multiple branch communication grooves 142. The multiple branch communication grooves 142 are arranged in one-to-one correspondence with the multiple first ports 12, and the multiple first ports 12 are communicated with the common port 11 through the main communication groove 141 and the multiple branch communication grooves 142.

[0055] In this way, the liquid can enter from the common port 11 and flow out from the multiple first ports 12 through the main communication groove 141 and the multiple groups of branch communication grooves 142, so as to realize the diversion of a single liquid.

[0056] Specifically, the number of the main communication grooves 141 is one, and the number of the branch communication grooves 142 can be three, four, five, six or more. For example, the common port 11 is communicated with the main communication groove 141, the number of the first ports 12 is six, the number of the branch communication grooves 142 is three groups, each group of branch communication grooves 142 includes two branch communication grooves 142, one ends of the three groups of branch communication grooves 142 are arranged in one-to-one correspondence with the six first ports 12, and the other ends are communicated with the main communication groove 141; it can also be that the number of the branch communication grooves 142 is two groups, each group of branch communication grooves 142 includes three branch communication grooves 142, one ends of the two groups of branch communication grooves 142 are arranged in one-to-one correspondence with the six first ports 12, and the other ends are communicated with the main communication groove 141. The main communication groove 141 and the branch communication grooves 142 can be in a bent shape, a curved shape, etc. The specific shapes of the main communication groove 141 and the branch communication grooves 142 are not limited herein.

[0057] Please refer to Figure 1 , in some embodiments, the multiple groups of branch communication grooves 142 include a first group of branch communication grooves 142 and a second group of branch communication grooves 142; the first group of branch communication grooves 142 is communicated with the main communication groove 141 through a first flow path 143; the second group of branch communication grooves 142 is communicated with the main communication groove 141 through a second flow path 144.

[0058] In this way, the liquid can enter the first group of branch communication grooves 142 and the second group of branch communication grooves 142 respectively from the main communication groove 141, so as to realize the diversion of a single liquid.

[0059] Specifically, the first set of branch communication grooves 142 and the second set of branch communication grooves 142 may include the same number of branch communication grooves 142, or may include different numbers of branch communication grooves 142. For example, both the first set of branch communication grooves 142 and the second set of branch communication grooves 142 include three branch communication grooves 142; for another example, the first set of branch communication grooves 142 includes two branch communication grooves 142, and the second set of branch communication grooves 142 includes three branch communication grooves 142. The first flow path 143 and the second flow path 144 may be communication grooves in the shape of a bend, a curve, etc., and are not specifically limited herein. One end of the first flow path 143 is connected to the main communication groove 141, and the other end communicates with the first set of branch communication grooves 142. The end of the first set of branch communication grooves 142 away from the first flow path 143 communicates with the first port 12; similarly, one end of the second flow path 144 is connected to the main communication groove 141, and the other end communicates with the second set of branch communication grooves 142. The end of the second set of branch communication grooves 142 away from the second flow path 144 communicates with the first port 12.

[0060] In one embodiment, both the first set of branch communication grooves 142 and the second set of branch communication grooves 142 include two branch communication grooves 142. The first set of branch communication grooves 142 and the second set of branch communication grooves 142 are symmetrically arranged with respect to the main communication groove 141, and the two branch communication grooves 142 of the first set of branch communication grooves 142 are symmetrically arranged, and the two branch communication grooves 142 of the second set of branch communication grooves 142 are symmetrically arranged, so that the flow distances of the liquid from the common port 11 to the four first ports 12 are equal, so that the liquid can reach the first ports 12 simultaneously at the same flow rate, maintaining the consistency of the liquid inlet and improving the sequencing efficiency.

[0061] Please refer to Figure 1 , in some embodiments, a liquid inlet end is provided on the main communication groove 141, and a liquid outlet end is provided on each branch communication groove 142. The liquid inlet end communicates with the common port 11, and the liquid outlet end communicates with the first port 12.

[0062] In this way, it is convenient for the liquid to enter the flow path selection valve 10 from the common port 11 through the liquid inlet end and flow out of the flow path selection valve 10 from the first port 12 through the liquid outlet end, realizing the liquid inlet and outlet of the fluid.

[0063] Specifically, the liquid inlet end may be located at one end of the main communication groove 141 away from the branch communication groove 142, or may be located at the middle position of the main communication groove 141 along the length direction, and the liquid outlet end is located at one end of the branch communication groove 142 away from the main communication groove 141.

[0064] Please refer to Figure 2 , in some embodiments, there are multiple second communication grooves 15. Each second communication groove 15 has two ends. One end of each second communication groove 15 communicates with one first port 12, and the other end communicates with one second port 13.

[0065] In this way, the liquid in each first port 12 can flow out from the corresponding second port 13 through the corresponding second communication groove 15, so as to realize the individual control of multiple liquids, avoid cross-contamination between multiple liquids, and improve the sequencing quality and sequencing efficiency.

[0066] Specifically, the second communication groove 15 can be a linear groove, and the length of the second communication groove 15 is the distance between the first port 12 and the corresponding second port 13. The first communication groove 14 and the second communication groove 15 can be arranged at intervals, and multiple second communication grooves 15 can be arranged at intervals with each other.

[0067] Please refer to Figure 3 , in some embodiments, the flow path selection valve 10 includes a stator 16 and a rotor 17 disposed opposite to the stator 16. The stator 16 is provided with a common port 11, a plurality of first ports 12 and a plurality of second ports 13, and the rotor 17 is provided with a first communication groove 14 and a second communication groove 15.

[0068] In this way, by rotating the rotor 17, the first communication groove 14 can connect the first port 12 with the common port 11, or the second communication groove 15 can connect the first port 12 with the second port 13, so as to realize the control of liquid flow path switching.

[0069] Specifically, the stator 16 and the rotor 17 can be coaxially arranged, that is to say, the central axis of the stator 16 coincides with the central axis of the rotor 17. It can be understood that the rotor 17 can rotate relative to the stator 16. Further, the rotor 17 rotates between a first valve position and a second valve position relative to the stator 16. When the rotor 17 is in the first valve position relative to the stator 16, the first port 12 is connected to the common port 11. When the rotor 17 is in the second valve position relative to the stator 16, the first port 12 is connected to the second port 13. When the rotor 17 is between the first valve position and the second valve position relative to the stator 16, the common port 11, the first port 12 and the second port 13 are mutually blocked. Unless otherwise specified, the "blocking" of multiple ports referred to in this article means that multiple ports cannot be connected, that is, liquid cannot enter from a specified one or more ports and flow out from a specified one or more other ports.

[0070] The common port 11, the first port 12 and the second port 13 can be circular through holes, and the through holes penetrate the stator 16 along the thickness direction of the stator 16. The first communication groove 14 and the second communication groove 15 can be in a bent shape, a curved shape, etc. The specific shapes of the first communication groove 14 and the second communication groove 15 are not limited herein.

[0071] Please refer to Figure 1, in some embodiments, the number of the common ports 11 is multiple. When the flow path selection valve 10 is in the first valve position, each common port 11 communicates with a plurality of first ports 12 through a first communication groove 14.

[0072] In this way, the liquids in the multiple common ports 11 can flow to the first ports 12 through the first communication groove 14 simultaneously, reducing the liquid inlet time and thus improving the liquid inlet efficiency.

[0073] Specifically, the number of the common ports 11 can be two. One of the common ports 11 communicates with the sample to be tested, and the other common port 11 communicates with the reagent, so that the sample to be tested and the reagent can flow in the first communication groove 14 simultaneously. The first port 12 can communicate with one of the common ports 11 or can communicate with each common port 11 simultaneously.

[0074] Please refer to Figure 4 , an embodiment of the present application provides a liquid path system 100, which includes a flow cell 20, a flow path selection valve 10 and a power assembly 30. The flow cell 20 includes a plurality of fluid channels 21, and the fluid channels 21 are used for carrying the sample to be tested and / or the reagent; the flow path selection valve 10 is arranged upstream of the flow cell 20, and a plurality of second ports 13 of the flow path selection valve 10 communicate with the plurality of fluid channels 21 and are arranged in one-to-one correspondence; the power assembly 30 is used to provide power to enable the sample to be tested and / or the reagent to enter the fluid channels 21 through the flow path selection valve 10.

[0075] In this way, the sample to be tested and / or the reagent can enter the plurality of fluid channels 21 through the flow path selection valve 10 and the power assembly 30, realizing the sequential determination of the sample to be tested.

[0076] Specifically, the flow cell 20 is used to provide a place for biochemical reactions during sequencing. The flow cell 20 can also be called a chip, and the flow cell 20 can be detachably connected to the flow path selection valve 10. The fluid channel 21 has a space for accommodating liquid, which can accommodate the sample to be tested and the reagent, enabling the sample to be tested to react biochemically with the reagent. The sample to be tested can be fixed on the inner surface of the fluid channel 21. The plurality of fluid channels 21 in the flow cell 20 are arranged in parallel. The number of the fluid channels 21 can be three, four, five, six, etc. The sizes of each fluid channel 21 can be exactly the same or different. In some embodiments, the fluid channel 21 has a non-circular cross-section or an approximately rectangular cross-section. The fluid channel 21 has a width of at least 2 mm, about 4 mm or about 7 mm, and the height of the fluid channel 21 can be at least 0.8 mm, about 1.2 mm.

[0077] The sample to be tested includes at least one nucleic acid molecule, and the reagent includes a polymerase and at least one nucleotide molecule. The polymerase is used to bind the nucleotide molecule to the nucleic acid molecule. The base types among multiple nucleotide molecules can be the same or different. The nucleotide molecule is provided with a blocking group, which is used to block the binding of more than one nucleotide to the nucleic acid molecule. The blocking group is provided with an optically detectable label, and the optically detectable label can enable the reaction between the nucleotide molecule and the nucleic acid molecule to be detected, so as to realize the sequence determination of the nucleic acid molecule.

[0078] Each fluid channel 21 is provided with an inlet and an outlet. Each second port 13 of the flow path selection valve 10 is connected to the inlet of a fluid channel 21, and the power assembly 30 enables the sample to be tested and / or the reagent from the second port 13 to enter the fluid channel 21 from the inlet. The power assembly 30 includes, but is not limited to, a device capable of providing power and a pipeline for transmitting power, and the power assembly 30 can be connected to the fluid channel 21 through a pipeline.

[0079] Please refer to Figure 4 , in some embodiments, the liquid path system 100 includes a first memory 40. The first memory 40 communicates with the common port 11 of the flow path selection valve 10, and the first memory 40 is used to store the sample to be tested and / or the reagent.

[0080] In this way, the sample to be tested and / or the reagent in the first memory 40 can enter the flow path selection valve 10 through the common port 11, and then flow to the fluid channel 21 of the flow cell 20, so as to realize the sequence determination of the sample to be tested.

[0081] Specifically, the first memory 40 can be a pipeline or a reagent bottle. The number of the first memories 40 can be one or two, or multiple. Each first memory 40 stores a sample to be tested or a reagent. Different first memories 40 can have the same or variable volumes. For example, each first memory 40 can have the same volume, or each first memory 40 can have different volumes, which can be set according to the required storage volume of the liquid stored in each first memory 40. The first memory 40 and the common port 11 can be communicated through a pipeline.

[0082] Please refer to Figure 4, in some embodiments, the power assembly 30 includes a first pump group 31 and a second pump group 32. The first pump group 31 is disposed upstream of the flow cell 20 and communicates with the first port 12 of the flow path selection valve 10. The first pump group 31 is selectively communicable with the flow path selection valve 10 and the first memory 40. The first pump group 31 is configured to aspirate the sample to be tested and / or the reagent in a negative pressure driving manner, and cause the sample to be tested and / or the reagent to enter the fluid channel 21 in a positive pressure driving manner; the second pump group 32 is disposed downstream of the flow cell 20, and the second pump group 32 is configured to cause the sample to be tested and / or the reagent aspirated by the first pump group 31 to enter the fluid channel 21 in a negative pressure driving manner.

[0083] In some embodiments, the first pump group 31 is configured to first aspirate the sample to be tested and / or the reagent in a negative pressure driving manner; and then cause the aspirated sample to be tested and / or the reagent to enter the fluid channel 21 in a positive pressure driving manner and simultaneously in combination with the second pump group 32 in a negative pressure driving manner.

[0084] Thus, by first aspirating the sample to be tested and / or the reagent by the first pump group 31 in a negative pressure driving manner, and then causing the sample to be tested and / or the reagent to enter the fluid channel 21 in a positive pressure driving manner and simultaneously in combination with the second pump group 32 to provide power, it is beneficial to increase the flow rate of the sample to be tested and / or the reagent, and at the same time, it avoids the adverse effects caused by the single negative pressure driving method for increasing the flow rate, which is likely to precipitate bubbles in the sample to be tested and / or the reagent or introduce external gas into the sample to be tested and / or the reagent to form bubbles; and it avoids the adverse effects caused by the single positive pressure driving method for increasing the flow rate, which may cause excessive pressure in the flow cell 20 and damage the flow cell 20.

[0085] Specifically, the first pump group 31 and the second pump group 32 can be peristaltic pumps, plunger pumps, syringe pumps, gear pumps or diaphragm pumps, etc. The first pump group 31 and the second pump group 32 can provide negative pressure or positive pressure. The first pump group 31 includes a plurality of first single pumps 33, and the plurality of first single pumps 33 correspond to the plurality of first ports 12 one by one. The second pump group 32 includes a plurality of second single pumps 34, and the plurality of second single pumps 34 correspond to the outlets of the plurality of fluid channels 21 one by one.

[0086] Please refer to Figure 4 , in some embodiments, the liquid path system 100 includes a plurality of first buffer regions 50, and each first buffer region 50 communicates with one first port 12 and one first single pump 33. When the flow path selection valve 10 is in the first valve position, the first pump group 31 is configured to aspirate a single sample to be tested and / or the reagent from the first memory 40 in a negative pressure driving manner, and pump the single sample to be tested and / or the reagent to each first buffer region 50.

[0087] In this way, the flow path selection valve 10 can divide a single sample and / or reagent to be tested entering from the common port 11 into multiple portions, such that the first memory 40 and the common port 11 can be communicated through a common pipeline. Compared with using a single positive pressure drive to make the sample and / or reagent to be tested enter the flow path selection valve 10 from the first memory 40 through multiple pipelines, the number of common pipelines is reduced, and the reagent consumption is lowered. In addition, the first buffer area 50 can store the sample and / or reagent to be tested from the first memory 40, facilitating the realization of the switching of the first pump group 31 from negative pressure drive to positive pressure drive.

[0088] Specifically, the first buffer area 50 can be a pipeline or a liquid storage bottle. The first pump group 31 provides negative pressure to drive a single sample and / or reagent in the first memory 40 to enter the flow path selection valve 10 from the common port 11, and then enter the corresponding first buffer area 50 from each first port 12.

[0089] Please refer to Figure 4 , in some embodiments, when the flow path selection valve 10 is in the second valve position, the first pump group 31 is used to pump the sample and / or reagent in each first buffer area 50 to the corresponding fluid channel 21 in a positive pressure drive manner; and / or, the second pump group 32 is used to pump the sample and / or reagent in each first buffer area 50 to the corresponding fluid channel 21 in a negative pressure drive manner.

[0090] Thus, the first pump group 31 first pumps the sample to be tested and / or reagent in the first memory 40 into each first buffer area 50 through the flow path selection valve 10 in a negative pressure manner. Since the sample to be tested and / or reagent does not pass through the fluid channel 21 when being pumped into each first buffer area 50 through the flow path selection valve 10, it will not be affected by the flow resistance in the fluid channel 21. Under the same negative pressure, compared with the flow rate of the sample to be tested and / or reagent directly entering the fluid channel 21 through the flow path selection valve 10 by the negative pressure generated by the second pump group 32, the sample to be tested and / or reagent can flow at a greater flow rate when entering each first buffer area 50 through the flow path selection valve 10 by the negative pressure generated by the first pump group 31. Then, the first pump group 31 pumps the sample to be tested and / or reagent in each first buffer area 50 to the corresponding fluid channel 21 in a positive pressure manner and / or the second pump group 32 pumps it in a negative pressure driving manner. Therefore, by first pumping the sample to be tested and / or reagent in the first memory 40 into each first buffer area 50 through the flow path selection valve 10 by the first pump group 31 in a negative pressure manner, and then pumping the sample to be tested and / or reagent in each first buffer area 50 to the corresponding fluid channel 21 in a positive pressure manner and / or the second pump group 32 pumps it in a negative pressure driving manner, it is beneficial to increase the flow rate of the sample to be tested and / or reagent, thus saving time and improving efficiency. Specifically, it can be that the first pump group 31 provides positive pressure to drive the sample to be tested and / or reagent in each first buffer area 50 to enter the flow path selection valve 10 from the corresponding first port 12, and then flow into the corresponding fluid channel 21 from the corresponding second port 13; it can also be that the second pump group 32 provides negative pressure to drive the sample to be tested and / or reagent in each first buffer area 50 to enter the flow path selection valve 10 from the corresponding first port 12, and then flow into the corresponding fluid channel 21 from the corresponding second port 13; it can also be that the first pump group 31 provides positive pressure drive and at the same time combines with the second pump group 32 to provide negative pressure drive for the sample to be tested and / or reagent in each first buffer area 50 to enter the flow path selection valve 10 from the corresponding first port 12, and then flow into the corresponding fluid channel 21 from the corresponding second port 13.

[0091] Please refer to Figure 4 , in some embodiments, the liquid path system 100 includes a plurality of first switching valves 60. The first switching valves 60 are disposed between the first pump group 31 and the flow path selection valve 10 and are used to control the on-off connection between the first pump group 31 and the flow path selection valve 10.

[0092] Thus, the first switching valve 60 can control the on-off connection between the first pump group 31 and the flow path selection valve 10, thereby controlling the flow of the liquid.

[0093] Specifically, the first switching valve 60 can be a solenoid valve. Each first switching valve 60 is connected to a first single pump 33 and a first port 12. Each first buffer area 50 communicates with a first port 12 and a first switching valve 60. When the first switching valve 60 is energized, the first pump group 31 is connected to the flow path selection valve 10. The first pump group 31 can drive a single test sample and / or reagent in the first memory 40 through the flow path selection valve 10 into multiple first buffer areas 50, or drive the test sample and / or reagent in each first buffer area 50 through the flow path selection valve 10 into the fluid channel 21. When the solenoid valve is de-energized, the connection between the first pump group 31 and the flow path selection valve 10 is disconnected.

[0094] Please refer to Figure 4 , in some embodiments, the liquid path system 100 includes multiple second buffer areas 51 and multiple second memories 41. Each second buffer area 51 communicates with a first single pump 33 and a second switching valve 61. The first pump group 31 is used to suck multiple test samples from multiple second memories 41 in a negative pressure driving manner and pump each test sample to a corresponding second buffer area 51.

[0095] In this way, the second buffer area 51 can store the test samples from the second memory 41, facilitating the switching of the first pump group 31 from negative pressure driving to positive pressure driving. The test samples in the second memory 41 can enter the flow path selection valve 10 through the second switching valve 61 and the first switching valve 60, and then flow to the fluid channel 21 of the flow cell 20 to realize the sequential determination of the test samples.

[0096] Specifically, the second buffer area 51 can be a pipeline or a liquid storage bottle. Each second buffer area 51 communicates with a first switching valve 60 and a first single pump 33. The first pump group 31 provides negative pressure to drive multiple test samples in multiple second memories 41 to enter multiple second buffer areas 51 through the second switching valve 61.

[0097] The second memory 41 can be a pipeline or a reagent bottle. The number of second memories 41 can be one or two, or multiple. Each second memory 41 stores one test sample. Different second memories 41 can have the same or varying volumes. For example, each second memory 41 can have the same volume, or each second memory 41 can have different volumes, which can be set according to the required storage volume of the test samples stored in each second memory 41. The second memory 41 and the second switching valve 61 can be connected through a pipeline.

[0098] Please refer to Figure 4, in some embodiments, when the flow path selection valve 10 is in the second valve position, the first pump group 31 is used to pump the test samples in each second buffer area 51 to the corresponding fluid channel 21 in a positive pressure driving manner; and / or, the second pump group 32 is used to pump the test samples in each second buffer area 51 to the corresponding fluid channel 21 in a negative pressure driving manner.

[0099] In this way, the first pump group 31 and / or the second pump group 32 can drive the test samples in each second buffer area 51 into the corresponding fluid channel 21, realizing the simultaneous injection of multiple test samples.

[0100] Specifically, it can be that the first pump group 31 provides positive pressure to drive the test samples in each second buffer area 51 to enter the flow path selection valve 10 from the corresponding first port 12 through the corresponding first switching valve 60, and then flow into the corresponding fluid channel 21 from the corresponding second port 13; it can also be that the second pump group 32 provides negative pressure to drive the test samples in each second buffer area 51 to enter the flow path selection valve 10 from the corresponding first port 12 through the corresponding first switching valve 60, and then flow into the corresponding fluid channel 21 from the corresponding second port 13; it can also be that the first pump group 31 provides positive pressure drive and at the same time combines with the second pump group 32 to provide negative pressure drive to drive the test samples in each second buffer area 51 to enter the flow path selection valve 10 from the corresponding first port 12 through the corresponding first switching valve 60, and then flow into the corresponding fluid channel 21 from the corresponding second port 13.

[0101] Please refer to Figure 4 , in some embodiments, the liquid path system 100 includes a plurality of second switching valves 61, and each second switching valve 61 is arranged between the corresponding second memory 41 and the second buffer area 51 for controlling the on-off connection between the second memory 41 and the second buffer area 51.

[0102] In this way, the second switching valve 61 can control the on-off connection between the second memory 41 and the second buffer area 51, thereby controlling the flow of the test samples.

[0103] Specifically, the second switching valve 61 can be a solenoid valve, and each second switching valve 61 is connected to the test samples in a first single pump 33 and a second memory 41. When the second switching valve 61 is energized, the second memory 41 is communicated with the second buffer area 51, and the first pump group 31 can drive the test samples in each second memory 41 into the corresponding second buffer area 51; when the solenoid valve is de-energized, the communication between the second memory 41 and the second buffer area 51 is disconnected. The second memory 41 is connected to the second switching valve 61, and the connection methods include but are not limited to barbs, threaded joints, etc.

[0104] Please refer to Figure 4, in some embodiments, the liquid path system 100 includes a rotary valve 70, and the rotary valve 70 communicates with a common port 11 of the first memory 40 and the flow path selection valve 10.

[0105] In this way, the rotary valve 70 can selectively communicate the first memory 40 and the common port 11 to achieve the switching of the sample or reagent to be tested and the switching between different reagents.

[0106] Specifically, the rotary valve 70 can rotate along its own axis to communicate the common port 11 with different first memories 40, facilitating the entry of different samples or reagents to be tested into the flow path selection valve 10. The first memory 40 is connected to the rotary valve 70, and the connection methods include but are not limited to barbs, threaded joints, etc.

[0107] In one embodiment, the liquid path system 100 includes a waste liquid bottle 80. The waste liquid bottle 80 is connected to the second pump group 32, and the waste liquid bottle 80 is used to store and discharge all or part of the waste liquid of the liquid path system 100. The second pump group 32 can suck the waste liquid from each fluid channel 21 in a negative pressure driving manner and then pump the sucked waste liquid to the waste liquid bottle 80 in a positive pressure driving manner.

[0108] In the description of this specification, the description with reference to terms such as "one embodiment", "some embodiments", "schematic embodiments", "examples", "specific examples", or "some examples" means that the specific features, structures, materials, or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic expressions of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described can be combined in a suitable manner in any one or more embodiments or examples.

[0109] Although the embodiments of the present invention have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and purposes of the present invention. The scope of the present invention is defined by the claims and their equivalents.

Claims

1. A flow path selection valve, characterized in that: The flow path selection valve is configured to switch between a first valve position and a second valve position. The flow path selection valve is provided with a common port, a plurality of first ports, a plurality of second ports, a first communicating groove, and a second communicating groove. When the flow path selection valve is in the first valve position, the plurality of first ports are communicated with the common port through the first communicating groove. When the flow path selection valve is in the second valve position, the first ports are communicated with the second ports in a one-to-one correspondence through the second communicating groove.

2. The flow path selection valve according to claim 1, characterized in that: The first communicating groove comprises a main communicating groove and a plurality of branch communicating grooves, the plurality of branch communicating grooves are all connected to the main communicating groove, and the plurality of branch communicating grooves are arranged in one-to-one correspondence with the plurality of the first ports; The plurality of first ports are in communication with the common port through the main communication groove and the plurality of branch communication grooves.

3. The flow path selection valve according to claim 1, characterized in that: The first communicating groove includes a main communicating groove and a plurality of groups of branch communicating grooves, the plurality of groups of branch communicating grooves are connected to the main communicating groove, and the plurality of groups of branch communicating grooves include a plurality of branch communicating grooves, and the plurality of branch communicating grooves are arranged in a one-to-one correspondence with the plurality of the first ports; The plurality of first ports are connected to the common port through the main connecting groove and the plurality of branch connecting grooves; Optionally, the plurality of groups of branch communication grooves include a first group of branch communication grooves and a second group of branch communication grooves; The first group of branch communication grooves are connected to the main communication groove through a first flow path; The second group of branch communication grooves are communicated with the main communication groove through a second flow path.

4. The flow path selection valve according to claim 2 or 3, characterized in that: A liquid inlet is arranged on the main connecting groove, and a liquid outlet is arranged on each of the branch connecting grooves. The liquid inlet is connected to the common port, and the liquid outlet is connected to the first port.

5. The flow path selection valve according to any one of claims 1 to 4, characterized in that: The second communicating grooves include a plurality of grooves, each of which has two ends, one end of each of which is connected to one of the first ports, and the other end of each of which is connected to one of the second ports.

6. The flow path selection valve according to any one of claims 1 to 5, characterized in that: The flow path selection valve includes a stator and a rotor disposed opposite to the stator, the stator is provided with the common port, the plurality of first ports, and the plurality of second ports, and the rotor is provided with the first communication groove and the second communication groove.

7. The flow path selection valve according to any one of claims 1 to 6, characterized in that: The number of the common ports is plural, and when the flow path selection valve is in the first valve position, each of the common ports is communicated with the plurality of the first ports through the first communication groove.

8. A liquid circuit system, characterized in that: include: A flow cell, the flow cell comprising a plurality of fluid channels, the fluid channels being used to carry samples to be tested and / or reagents; The flow path selection valve according to any one of claims 1 to 7, wherein the flow path selection valve is arranged upstream of the flow pool, and a plurality of second ports of the flow path selection valve are connected to a plurality of the fluid channels and are arranged one by one; The power assembly is used to provide power to enable the sample to be tested and / or the reagent to enter the fluid channel through the flow path selection valve.

9. The fluid circuit system according to claim 8, characterized in that: The fluid path system comprises a first storage device, the first storage device is connected to a common port of the flow path selection valve, and the first storage device is used to store the sample to be tested and / or the reagent; Optionally, the power assembly includes a first pump group and a second pump group, the first pump group is arranged upstream of the flow pool and communicated with the first port of the flow path selection valve, the first pump group is selectively communicated with the flow path selection valve and the first storage device, and the first pump group is used to suck the sample to be tested and / or the reagent in a negative pressure driven manner, and to make the sample to be tested and / or the reagent enter the fluid channel in a positive pressure driven manner; The second pump group is arranged downstream of the flow pool, and is used to drive the sample to be tested and / or the reagent sucked by the first pump group into the fluid channel in a negative pressure driven manner; Optionally, the fluid path system includes a plurality of first buffer areas, each of which is connected to one of the first ports and the first pump group, and when the flow path selection valve is in the first valve position, the first pump group is used to draw a single sample to be tested and / or the reagent from the first storage in a negative pressure driven manner, and pump the single sample to be tested and / or the reagent to each of the first buffer areas; Optionally, when the flow path selection valve is in the second valve position, the first pump group is used to pump the sample to be tested and / or the reagent in each first buffer area to the corresponding fluid channel in a positive pressure driven manner; and / or, The second pump group is used to pump the sample to be tested and / or the reagent in each first buffer area to the corresponding fluid channel in a negative pressure driven manner.

10. The fluid circuit system according to claim 9, characterized in that: The liquid circuit system includes a plurality of first switching valves, wherein the first switching valve is arranged between the first pump group and the flow path selection valve, and is used to control the on-off connection between the first pump group and the flow path selection valve; Optionally, the fluid path system includes a plurality of second buffer areas and a plurality of second storages, each of the second buffer areas is connected to the first pump group and a second switching valve, the first pump group is used to draw a plurality of the samples to be tested from the second storage in a negative pressure driven manner, and pump each of the samples to be tested to a corresponding second buffer area; Optionally, when the flow path selection valve is in the second valve position, the first pump group is used to pump the sample to be tested in each second buffer area to the corresponding fluid channel in a positive pressure driven manner; and / or, The second pump group is used to pump the sample to be tested in each second buffer area to the corresponding fluid channel in a negative pressure driven manner; Optionally, the fluid circuit system includes a plurality of second switching valves, each of which is disposed between the corresponding second storage and the second buffer area, and is used to control the on-off connection between the second storage and the second buffer area; Optionally, the fluid path system comprises a rotary valve, and the rotary valve is connected to a common port of the first reservoir and the flow path selection valve.

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