Pretreatment instrument based on nano magnetic bead extraction method

Abstract

The utility model discloses a pretreatment instrument based on a nano magnetic bead extraction method. The pretreatment instrument comprises a case, a sample carrying table, a sample rack, a detection test tube, a support, a magnetic suction device, a stirring device and a blower device, the case is provided with a sample bin, the sample bin is provided with a sample carrying table, and the sample carrying table is provided with an X-axis moving device; the transmission end of the X-axis moving device is in transmission connection with the sample carrying table; the magnetic attraction device attracts the nano magnetic beads or releases the nano magnetic beads through magnetic force changes; the stirring device is used for stirring liquid; and the blower device is arranged on the support. The pretreatment instrument is provided with a magnetic attraction device, a stirring device, an air blowing device and an X-axis moving device, and nano magnetic beads are transferred in a magnetic bead treatment pipe, a sample groove and an elution pipe through the magnetic attraction device, so that the nano magnetic beads can adsorb target objects, such as drugs, antibiotics, pesticide residues, veterinary drugs and novel organic pollutants, existing in a water sample; and a target object is transferred into the eluent, and the water sample is pretreated in time, so that the timeliness of water sample detection is enhanced.

Description

Technical Field

[0001] The utility model relates to the field of sample treatment devices, in particular to a pretreatment instrument based on the nano magnetic bead extraction method. Background Art

[0002] The monitoring method of collecting domestic sewage from sewage treatment plants to detect the content of drugs and their human metabolites therein is an important technical means for the public security anti-drug department to master the types and levels of drug abuse in the jurisdiction and evaluate the number of drug users. When the sample monitoring data of the sewage treatment plant is abnormal, it indicates the suspicion of group drug use or drug manufacturing. The public security anti-drug department needs to carry out sewage traceability monitoring, that is, to set up monitoring points step by step along the sewage pipe network from the sewage treatment plant, continuously narrow the scope until the suspected communities, places, etc. are locked.

[0003] However, according to the conventional technical means, after collecting sewage samples from the traceability site, they must be taken to a special laboratory for sample pretreatment to concentrate and enrich the target substances therein, and then instrumental analysis can be carried out. This process consumes a large amount of manpower and material resources, and the timeliness is extremely poor, and it simply cannot meet the actual combat needs of public security case handling. Content of the Utility Model

[0004] The purpose of the utility model is to provide a pretreatment instrument based on the nano magnetic bead extraction method to solve the problem that the above technical means cannot pretreat samples in time, resulting in poor timeliness of sewage traceability monitoring.

[0005] To achieve this purpose, the utility model adopts the following technical solutions:

[0006] The utility model provides a pretreatment instrument based on the nano magnetic bead extraction method, including a chassis, a sample stage, a sample rack, a detection test tube, a support, a magnetic attraction device, a stirring device and a blowing device;

[0007] The chassis is provided with a sample chamber, the sample chamber is provided with a sample stage, and the sample stage is provided with an X-axis moving device; the transmission end of the X-axis moving device is in transmission connection with the sample stage to drive the sample stage to move along the X-axis direction;

[0008] The sample rack is placed on the sample stage, and the detection test tube is fixed in the sample rack; the detection test tube includes a parallel connection plate, a sample groove, an elution tube and a magnetic bead treatment tube group; the sample groove, the magnetic bead treatment tube group and the elution tube are arranged on the parallel connection plate in sequence along the X-axis direction; the sample groove is used for placing water samples; the magnetic bead treatment tube group includes two or more magnetic bead treatment tubes, and the magnetic bead treatment tubes are respectively filled with reagents required for treating magnetic beads before and after adsorbing the target substances, and the elution tube is filled with an elution solution;

[0009] The magnetic attraction device sucks or releases the nano magnetic beads through magnetic force changes, enabling the nano magnetic beads to enter the magnetic bead processing tube, the sample tank, and the elution tube in sequence; the stirring device is used to stir the liquid in the sample tank, the magnetic bead processing tube, or the elution tube; the air blowing device is arranged on the support and is used to blow dry the eluent.

[0010] In the pretreatment instrument for the nano magnetic bead extraction method, the magnetic attraction device includes a magnetic attraction motion module, a magnetic attraction arm, and a magnetic attraction rod; the magnetic attraction motion module is arranged on the support; the magnetic attraction rod is arranged on the magnetic attraction arm;

[0011] The stirring device includes a stirring motion module, a stirring arm, and a stirring sleeve; the stirring motion module is arranged on the support, and the stirring motion module is located on one side of the magnetic attraction motion module; the stirring sleeve is arranged on the stirring arm; the driving end of the stirring motion module is connected to the side surface of the stirring arm, and the stirring arm is located directly below the magnetic attraction arm, and the stirring sleeve is located directly below the projection of the magnetic attraction rod in the vertical direction;

[0012] The stirring motion module drives the stirring arm and the stirring sleeve to move along the Z-axis direction; the driving end of the magnetic attraction motion module is connected to the magnetic attraction arm to drive the magnetic attraction arm and the magnetic attraction rod to move along the Z-axis direction and make the magnetic attraction rod insert into or leave the stirring sleeve.

[0013] In the pretreatment instrument for the nano magnetic bead extraction method, the stirring arm is provided with a slot along the Y-axis direction, the slot is located at one end of the stirring arm away from the stirring motion module, and an avoidance opening is provided below the slot; an insertion opening communicating with the slot is provided at the top of the stirring arm;

[0014] The stirring sleeve includes a stirring sleeve beam, a stirring sleeve handle, and a stirring sleeve tube; the stirring sleeve handle is connected to one end of the stirring sleeve beam, the stirring sleeve tube is connected to the stirring sleeve beam, and the position of the stirring sleeve tube is adapted to that of the magnetic attraction rod; the stirring sleeve beam is inserted into the slot, and the stirring sleeve tube passes through the slot through the avoidance opening.

[0015] In the pretreatment instrument for the nano magnetic bead extraction method, the air blowing device includes an air blowing motion module, an air blowing arm, a blower, and an air blowing needle; the air blowing motion module is arranged on the support, and the air blowing motion module is located on one side of the magnetic attraction motion module; the air blowing needles are respectively arranged on the air blowing arm, and the air outlet of the blower is communicated with the air inlet of the air blowing needle; the driving end of the air blowing motion module is connected to the air blowing arm to drive the air blowing arm and the air blowing needle to move along the Z-axis direction; the air blowing needle is located above the test tube.

[0016] In the pretreatment instrument for the nano magnetic bead extraction method, the magnetic attraction motion module, the stirring motion module, and the air blowing motion module have the same structure, including a Z-axis driving member, a Z-axis screw rod, a Z-axis sliding nut, a Z-axis connecting plate, and a Z-axis guide rail;

[0017] The Z-axis screw is arranged on the support along the Z-axis direction. One end of the Z-axis screw passes through the support and is in transmission connection with the driving end of the Z-axis driving member; the other end of the Z-axis screw is rotatably connected to the support; the Z-axis sliding nut is threadedly assembled with the Z-axis screw; one side of the Z-axis connecting plate is connected to the Z-axis screw, and a connecting arm extends forward on the other side of the Z-axis connecting plate, and the connecting arm is connected to the magnetic attraction arm, the stirring arm or the air blowing arm;

[0018] The Z-axis guide rail is arranged on the support along the Z-axis direction. A Z-axis slider is further provided on the other side of the Z-axis connecting plate, and the Z-axis slider is slidably assembled with the Z-axis guide rail; the Z-axis driving member is arranged on the support and drives the Z-axis screw to rotate about its own axis, so that the Z-axis sliding nut and the Z-axis connecting plate rise or fall.

[0019] In the pretreatment instrument for the nano magnetic bead extraction method, the magnetic bead treatment tube group includes a first treatment tube, a second treatment tube and a third treatment tube, and the first treatment tube, the second treatment tube and the third treatment tube are sequentially arranged on the parallel plate along the X-axis direction;

[0020] The first treatment tube is filled with nano magnetic bead activation liquid; the second treatment tube is filled with nano magnetic bead balancing liquid, and the third treatment tube is filled with nano magnetic bead washing liquid.

[0021] In the pretreatment instrument for the nano magnetic bead extraction method, the X-axis moving device includes an X-axis driving member, an X-axis screw, an X-axis sliding nut, an X-axis connecting plate and an X-axis guide rail;

[0022] The X-axis screw is arranged on the sample stage along the X-axis direction, and both ends of the X-axis screw are respectively rotatably connected to the sample stage; one end of the X-axis screw is in transmission connection with the driving end of the X-axis driving member; the X-axis sliding nut is threadedly assembled with the X-axis screw; one side of the X-axis connecting plate is connected to the X-axis sliding nut; the other side of the X-axis connecting plate is connected to the support;

[0023] The X-axis guide rail is arranged on the sample stage along the X-axis direction. An X-axis slider is provided on the other side of the X-axis connecting plate, and the X-axis slider is slidably assembled with the X-axis guide rail;

[0024] The X-axis driving member is arranged on the sample stage and drives the X-axis screw to rotate about its own axis, so that the X-axis sliding nut and the X-axis connecting plate move along the X-axis direction.

[0025] In the pretreatment instrument for the nano magnetic bead extraction method, the sample stage is further provided with a heating device, and the sample rack is arranged on the outer periphery of the heating device; the heating device is arranged at the bottom of the sample stage, and the heating end of the heating device passes through the sample stage; the elution tube is inserted into the heating end of the heating device.

[0026] In the pretreatment instrument using the nano magnetic bead extraction method, an air filter is provided on the chassis.

[0027] In the pretreatment instrument using the nano magnetic bead extraction method, a control device is further included. The control device includes a control module and a control panel. The control module is arranged on the top of the chassis and is electrically connected to the magnetic attraction device, the stirring device, and the air blowing device respectively for controlling the magnetic attraction device, the stirring device, and the air blowing device.

[0028] The control panel is arranged on the outer side of the chassis and is electrically connected to the control device.

[0029] One technical solution in the present utility model can have the following beneficial effects:

[0030] The pretreatment instrument is provided with a magnetic attraction device, a stirring device, an air blowing device, and an X-axis moving device. Through the magnetic attraction device, the nano magnetic beads are transferred in the magnetic bead treatment tube, the sample tank, and the elution tube, so that the nano magnetic beads can adsorb the target substances existing in the water sample and transfer the target substances to the eluent, enabling timely pretreatment of the water sample, facilitating subsequent detection, improving the detection efficiency, and enhancing the timeliness of water sample detection. The water sample does not need to pass through a solid phase extraction column and there is no risk of blockage, so there is no need for filtration, saving time and cost. Moreover, it is small in size and easy to carry to the sample collection site for sample pretreatment. Brief Description of the Drawings

[0031] Figure 1 is a schematic structural diagram of one embodiment of the present utility model;

[0032] Figure 2 is an internal structural diagram of one embodiment of the present utility model;

[0033] Figure 3 is a positional relationship diagram of the sample rack, the detection test tube, the support, the magnetic attraction device, the stirring device, and the air blowing device in one embodiment of the present utility model;

[0034] Figure 4 is Figure 3 a positional relationship diagram of the other side of the embodiment;

[0035] Figure 5 is a schematic structural diagram of the magnetic attraction device, the stirring device, and the air blowing device in one embodiment of the present utility model;

[0036] Figure 6 is a connection relationship diagram of the stirring arm and the stirring sleeve in one embodiment of the present utility model;

[0037] Figure 7 is a schematic structural diagram of the stirring sleeve in one embodiment of the present utility model;

[0038] Figure 8 It is a schematic structural diagram of a detection test tube in one embodiment of the present utility model;

[0039] In the attached drawings: chassis 1, sample stage 2, sample rack 3, detection test tube 4, support 5, magnetic attraction device 6, stirring device 7, air blowing device 8, control device 9;

[0040] Sample chamber 10, air filter 11; X-axis moving device 21; heating device 22; parallel plate 41, sample slot 42, elution tube 43, first treatment tube 44, second treatment tube 45, third treatment tube 46; magnetic attraction motion module 61, magnetic attraction arm 62, magnetic attraction rod 63; stirring motion module 71, stirring arm 72, stirring sleeve 73; air blowing motion module 81, air blowing arm 82, air blower 83, air blowing needle 84; control module 91, control panel 92;

[0041] X-axis driving member 211, X-axis screw 212, X-axis sliding nut 213, X-axis connecting plate 214, X-axis guide rail 215; X-axis slider 216; Z-axis driving member 601, Z-axis screw 602, Z-axis sliding nut 603, Z-axis connecting plate 604, Z-axis guide rail 605; connecting arm 606; Z-axis slider 607; slot 720; avoidance opening 721; insertion interface 722; stirring sleeve beam 731, stirring sleeve handle 732, stirring sleeve tube 733; fixing protrusion 734. Detailed implementation manners

[0042] The embodiments of the present utility model will be described in detail below. The examples of the embodiments are shown in the attached drawings, in which the same or similar reference numerals represent the same or similar elements or elements with the same or similar functions from beginning to end. The embodiments described below by referring to the attached drawings are exemplary and are only used to explain the present utility model and should not be construed as a limitation to the present utility model.

[0043] In the description of the present utility model, it should be understood that the orientation or positional relationship indicated by the terms "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the attached drawings, and is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present utility model. In addition, the features defined with "first" and "second" may explicitly or implicitly include one or more of such features, which are used to distinguish and describe features, without order or importance.

[0044] In the description of the present utility model, unless otherwise specified, the meaning of "a plurality of" is two or more.

[0045] In the description of the present utility model, it should be noted that unless otherwise clearly specified and defined, the terms "installation", "connection", and "coupling" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, and it can be the communication inside two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific situations.

[0046] Please refer to Figures 1 to 8 , the present utility model provides a pretreatment instrument based on the nano - magnetic bead extraction method, including a chassis 1, a sample - loading platform 2, a sample rack 3, a detection test tube 4, a support 5, a magnetic attraction device 6, a stirring device 7, and a blowing device 8;

[0047] The chassis 1 is provided with a sample chamber 10, the sample chamber 10 is provided with a sample - loading platform 2, and the sample - loading platform 2 is provided with an X - axis moving device 21; the driving end of the X - axis moving device 21 is in driving connection with the sample - loading platform 2 to drive the sample - loading platform 2 to move along the X - axis direction;

[0048] The sample rack 3 is placed on the sample - loading platform 2, and the detection test tube 4 is fixed in the sample rack 3; the detection test tube 4 includes a parallel - connection plate 41, a sample groove 42, an elution tube 43, and a magnetic bead treatment tube group; the sample groove 42, the magnetic bead treatment tube group, and the elution tube 43 are sequentially arranged along the X - axis direction on the parallel - connection plate 41; the sample groove 42 is used for placing water samples; the magnetic bead treatment tube group includes two or more magnetic bead treatment tubes, and the magnetic bead treatment tubes are respectively filled with reagents for treating nano - magnetic beads, and the elution tube 43 is filled with an eluent;

[0049] The magnetic attraction device 6 absorbs or releases nano - magnetic beads through magnetic force changes, so that the nano - magnetic beads sequentially enter the magnetic bead treatment tubes, the sample groove 42, and the elution tube 43; the stirring device 7 is used for stirring the liquid in the sample groove 42, the magnetic bead treatment tubes, or the elution tube 43; the blowing device 8 is arranged on the support 5 and is used for blowing dry the eluent.

[0050] The X-axis moving device 21 can drive the support 5 to move along the X-axis direction, so that the magnetic attraction device 6, the stirring device 7 and the air blowing device 8 can move to the positions of the sample tank 42, the magnetic bead treatment tube and the elution tube 43. The core of the nano magnetic beads is a superparamagnetic material, and the surface is modified with specific functional groups and spatial structures. The magnetic attraction device 6 can achieve the purpose of adsorbing nano magnetic beads by generating magnetic force, and can also release nano magnetic beads by canceling the magnetic force. The nano magnetic beads can be transferred into the magnetic bead treatment tube, the sample tank 42 and the elution tube 43 through the magnetic attraction device 6. The magnetic bead treatment tubes are respectively filled with the reagents required for treating the magnetic beads before and after adsorbing the target substance. The number of magnetic bead treatment tubes and the reagents contained therein can be adjusted according to the actual steps required for adsorbing the target substance and the reagents required in the steps. In a specific embodiment of the present utility model, it includes an activation solution, a balance solution and a washing solution, and the nano magnetic beads are activated, balanced and washed. In some specific embodiments, the nano magnetic beads need to be washed multiple times. Multiple magnetic bead treatment tubes filled with washing solution can be set according to the actual number of washing times; or the number of magnetic bead treatment tubes and the reagents filled in the magnetic bead treatment tubes can be selected according to the actual situation.

[0051] The nano magnetic beads can actively adsorb and bind the target substance from the water sample. The nano magnetic beads put into the sample tank 42 are adsorbed by the magnetic attraction device 6 and put into the magnetic bead treatment tube filled with washing solution for washing; then the nano magnetic beads put into the sample tank 42 are adsorbed by the magnetic attraction device 6 again and then put into the elution tube 43. The eluent elutes the target substance from the surface of the nano magnetic beads. Subsequently, the magnetic attraction rod 63 of the magnetic attraction device 6 sucks the nano magnetic beads away from the eluent, and the target substance remains in the eluent to complete the pretreatment; the staff can take out the eluent and return to the laboratory to detect the target substance in the eluent through an instrument to obtain the detection result, or part or all of the eluent can be volatilized by the air blowing device 8 to further concentrate the eluent or obtain the air-dried substance in the eluent, and then add specific reagents to perform rapid detection on-site by fluorescence immunochromatography, surface-enhanced Raman or other methods.

[0052] In some specific embodiments of the present utility model, the opening of the sample chamber 10 is arranged on the front side of the chassis 1, and a sample chamber door is hinged at the opening of the sample chamber 10. With the above structure, it is convenient for the detection personnel to put in the water sample. A handle can also be arranged on the top of the chassis 1 for easy carrying.

[0053] The pretreatment instrument is provided with a magnetic attraction device 6, a stirring device 7, an air blowing device 8 and an X-axis moving device 21. The magnetic attraction device 6 is used to transfer the nano magnetic beads in the magnetic bead treatment tube, the sample tank 42 and the elution tube 43, so that the nano magnetic beads can adsorb the target substance existing in the water sample and transfer the target substance to the eluent, and the water sample can be pretreated in time, which is convenient for subsequent detection, improves the detection efficiency, and enhances the timeliness of water sample detection. Moreover, it is small in volume and convenient to carry.

[0054] Specifically, the magnetic attraction device 6 includes a magnetic attraction motion module 61, a magnetic attraction arm 62, and a magnetic attraction rod 63; the magnetic attraction motion module 61 is disposed on the support 5; the magnetic attraction rod 63 is disposed on the magnetic attraction arm 62;

[0055] The stirring device 7 includes a stirring motion module 71, a stirring arm 72, and a stirring sleeve 73; the stirring motion module 71 is disposed on the support 5, and the stirring motion module 71 is located on one side of the magnetic attraction motion module 61; the stirring sleeve 73 is disposed on the stirring arm 72; the driving end of the stirring motion module 71 is connected to the side surface of the stirring arm 72, and the stirring arm 72 is located directly below the magnetic attraction arm 62, and the stirring sleeve 73 is located directly below the projection of the magnetic attraction rod 63 in the vertical direction;

[0056] The stirring motion module 71 drives the stirring arm 72 and the stirring sleeve 73 to move along the Z-axis direction; the driving end of the magnetic attraction motion module 61 is connected to the magnetic attraction arm 62 to drive the magnetic attraction arm 62 and the magnetic attraction rod 63 to move along the Z-axis direction, and to insert the magnetic attraction rod 63 into or out of the stirring sleeve 73.

[0057] When nano magnetic beads need to be transferred, the magnetic attraction motion module 61 drives the magnetic attraction arm 62 to descend, so that the magnetic attraction rod 63 is inserted into the stirring sleeve 73; subsequently, the magnetic attraction motion module 61 drives the magnetic attraction arm 62 to descend, and the stirring motion module 71 drives the stirring arm 72 to descend synchronously, so that the stirring sleeve 73 is inserted into the magnetic bead processing tube, the sample tank 42, or the elution tube 43; since the magnetic attraction rod 63 has magnetism, the nano magnetic beads will be adsorbed on the outer periphery of the stirring sleeve 73; then, the magnetic attraction motion module 61 drives the magnetic attraction arm 62 to rise, the stirring motion module 71 drives the stirring arm 72 to rise synchronously, and the X-axis moving device 21 drives the support 5 to move along the X-axis direction, so that the magnetic attraction rod 63 and the stirring sleeve 73 are moved above the sample tank 42, the elution tube 43, or another magnetic bead processing tube; the magnetic attraction motion module 61 drives the magnetic attraction arm 62 to descend again, the stirring motion module 71 drives the stirring arm 72 to descend synchronously again, so that the stirring sleeve 73 is inserted above the sample tank 42, the elution tube 43, or another magnetic bead processing tube; finally, the magnetic attraction motion module 61 drives the magnetic attraction arm 62 to rise; since the magnetic attraction rod 63 leaves the stirring sleeve 73, the magnetism of the stirring sleeve 73 disappears, and the nano magnetic beads are dispersed from the side wall of the stirring sleeve 73; after the nano magnetic beads are completely separated from the stirring sleeve 73, the stirring motion module 71 drives the stirring arm 72 to rise synchronously, and the transfer of the nano magnetic beads is completed.

[0058] The parallel plate 41 is provided with sample holes, elution holes, and a plurality of magnetic bead processing tube holes, the sample tank 42 is communicated with the sample holes; the elution tube 43 is communicated with the elution holes; the plurality of magnetic bead processing tube holes are respectively communicated with the corresponding magnetic bead processing tube holes.

[0059] After the stirring sleeve 73 is inserted into the sample cell 42, the X-axis moving device 21 drives the support 5 to move slightly in the X-axis direction, so that the stirring sleeve 73 reciprocates in the X-axis direction in the sample hole, thereby disturbing the water sample and achieving the purpose of stirring. In addition, the stirring motion module 71 can also drive the stirring sleeve 73 to reciprocate slightly in the Z-axis direction in the magnetic bead treatment tube or the elution tube, thereby disturbing the reagent or the eluent, so as to achieve the purpose of stirring.

[0060] With the above structure, the magnetic attraction rod 63 can be protected by the stirring sleeve 73, avoiding the problem that the magnetic attraction rod 63 is directly in contact with the water sample, reagent or eluent, resulting in corrosion or contamination of the magnetic attraction rod 63, and prolonging the service life of the magnetic attraction rod 63.

[0061] Specifically, the stirring arm 72 is provided with a slot 720 in the Y-axis direction. The slot 720 is located at one end of the stirring arm 72 away from the stirring motion module 71, and an avoidance opening 721 is provided below the slot 720; an insertion opening 722 communicating with the slot 720 is provided at the top of the stirring arm 72;

[0062] The stirring sleeve 73 includes a stirring sleeve beam 731, a stirring sleeve handle 732 and a stirring sleeve tube 733; the stirring sleeve handle 732 is connected to one end of the stirring sleeve beam 731, the stirring sleeve tube 733 is connected to the stirring sleeve beam 731, and the position of the stirring sleeve tube 733 is adapted to that of the magnetic attraction rod 63; the stirring sleeve beam 731 is inserted into the slot 720, and the stirring sleeve tube 733 passes through the slot 720 through the avoidance opening 721.

[0063] In a specific embodiment of the present invention, a plurality of fixing protrusions 734 are respectively arranged at intervals on both sides of the stirring sleeve beam 731 in the X-axis direction, so that the stirring sleeve beam 731 can be stuck on the avoidance opening 721 to achieve sliding assembly.

[0064] With the above structure, a detachable structure of the stirring arm 72 and the stirring sleeve 73 is realized. The stirring sleeve 73 is a disposable item. After the detection is completed, it can be pulled out from the slot 720 and a new stirring sleeve 73 can be replaced. The stirring sleeve 73 can be replaced, which can effectively avoid cross-contamination between samples. The stirring sleeve tube 733 is located below the magnetic attraction rod 63, so that the magnetic attraction rod 63 is inserted into the stirring sleeve 73. The stirring sleeve beam 731 is used to connect the stirring sleeve tube 733, and the stirring sleeve handle 732 is used to provide a holding position for the staff to replace the stirring sleeve 73. The stirring sleeve tube 733 is used to protect the magnetic attraction rod 63.

[0065] Further, the air blowing device 8 includes an air blowing motion module 81, an air blowing arm 82, a blower 83, and an air blowing needle 84; the air blowing motion module 81 is disposed on the support 5, and the air blowing motion module 81 is located on one side of the magnetic attraction motion module 61; the air blowing needles 84 are respectively disposed on the air blowing arm 82, and the air outlet of the blower 83 is communicated with the air inlet of the air blowing needle 84; the driving end of the air blowing motion module 81 is connected to the air blowing arm 82 to drive the air blowing arm 82 and the air blowing needle 84 to move along the Z-axis direction; the air blowing needle 84 is located above the test tube 4 for detection.

[0066] The air blowing motion module 81 is used to drive the air blowing arm 82 and the air blowing needle 84 to move along the Z-axis direction, so that the air blowing needle 84 is inserted into or away from the elution tube 43. When it is necessary to dry or partially volatilize the eluent, the air blowing motion module 81 drives the air blowing arm 82 and the air blowing needle 84 to descend, so as to insert into the elution tube 43 to a specified depth. The blower 83 generates an air flow, so that the air blowing needle 84 blows out the air flow to blow off all or part of the volatile solvents in the eluent, realizing concentration or drying. Subsequently, the air blowing motion module 81 drives the air blowing arm 82 and the air blowing needle 84 to rise, so that the air blowing needle 84 is away from the elution tube 43.

[0067] In a specific embodiment of the present utility model, the magnetic attraction device 6 includes a plurality of magnetic attraction rods 63, the magnetic attraction rods 63 are arranged at intervals on the magnetic attraction arm 62, the stirring sleeve 73 includes a plurality of stirring sleeve tubes 733, the stirring sleeve tubes 733 are arranged at intervals on the stirring sleeve beam 731, and the number and positions of the stirring sleeve tubes 733 are adapted to the number and positions of the magnetic attraction rods 63; in addition, one or more test tubes 4 for detection can be placed on the sample rack 3, the full-load quantity of the test tubes 4 for detection and the distance between adjacent test tubes 4 for detection are adapted to the number of the magnetic attraction rods 63 and the distance between adjacent magnetic attraction rods 63; the air blowing device 8 includes a plurality of air blowing needles 84, the air blowing needles 84 are arranged at intervals on the air blowing arm 82, and the number and positions of the air blowing needles 84 are adapted to the number and positions of the elution tubes 43. With the above structure, the pretreatment of multiple groups of water samples can be carried out simultaneously, further improving the efficiency of the pretreatment.

[0068] As a simple replacement, the blower 83 can be disposed on the air blowing arm 82, the side wall of the sample chamber 10, the sample loading table 2, the chassis 1 or the support 5.

[0069] Furthermore, the magnetic attraction motion module 61, the stirring motion module 71, and the air blowing motion module 81 have the same structure, including a Z-axis driving member 601, a Z-axis screw 602, a Z-axis sliding nut 603, a Z-axis connecting plate 604, and a Z-axis guide rail 605;

[0070] The Z-axis screw rod 602 is arranged along the Z-axis direction on the support 5. One end of the Z-axis screw rod 602 passes through the support 5 and is in transmission connection with the driving end of the Z-axis driving member 601; the other end of the Z-axis screw rod 602 is rotatably connected to the support 5; the Z-axis sliding nut 603 is threadedly assembled with the Z-axis screw rod 602; one side of the Z-axis connecting plate 604 is connected to the Z-axis screw rod 602, and a connecting arm 606 extends forward on the other side of the Z-axis connecting plate 604, and the connecting arm 606 is connected to the magnetic attraction arm 62, the stirring arm 72 or the air blowing arm 82;

[0071] The Z-axis guide rail 605 is arranged along the Z-axis direction on the support 5. A Z-axis slider 607 is further provided on the other side of the Z-axis connecting plate 604, and the Z-axis slider 607 is slidably assembled with the Z-axis guide rail 605; the Z-axis driving member 601 is arranged on the support 5 and drives the Z-axis screw rod 602 to rotate about its own axis, so that the Z-axis sliding nut 603 and the Z-axis connecting plate 604 move up or down.

[0072] The transmission principle of the Z-axis screw rod 602 and the Z-axis sliding nut 603 can refer to the ball screw, which converts the rotational motion into a linear motion. The spiral groove on the Z-axis screw rod 602 guides the Z-axis sliding nut 603 to move along the axial direction. Due to the limitation of the Z-axis guide rail 605 and the Z-axis slider 607, the Z-axis sliding nut 603 moves along the axial direction of the Z-axis screw rod 602.

[0073] Adopting the above transmission structure can transmit torque with higher efficiency, and has lower frictional resistance, has higher precision and repeat positioning accuracy, and can bear larger axial and radial loads, and has a longer service life.

[0074] In a specific embodiment of the present invention, a cavity is provided at the bottom of the support 5, the Z-axis driving member 601 is arranged downward, passes through the bottom of the support 5 and enters the cavity, the other end of the Z-axis screw rod 602 is rotatably connected to the bottom of the support 5, and passes through the bottom of the support 5 and enters the cavity; a first transmission belt is sleeved on the driving end of the Z-axis driving member 601 and the outer periphery of the other end of the Z-axis screw rod 602, and the driving end of the Z-axis driving member 601 drives the Z-axis screw rod 602 to rotate about its own axis through the first transmission belt.

[0075] Specifically, the magnetic bead treatment tube group includes a first treatment tube 44, a second treatment tube 45 and a third treatment tube 46, and the first treatment tube 44, the second treatment tube 45 and the third treatment tube 46 are sequentially arranged on the parallel plate 41 along the X-axis direction;

[0076] The first treatment tube 44 is filled with nano magnetic bead activation liquid; the second treatment tube 45 is filled with nano magnetic bead balancing liquid, and the third treatment tube 46 is filled with nano magnetic bead washing liquid.

[0077] The nano magnetic bead activation solution is used to activate the three-dimensional structure and functional groups on the surface of the nano magnetic beads, enabling the surface of the nano magnetic beads to adsorb the target substance; the nano magnetic bead equilibration solution is used to balance the micro-interface environment of the three-dimensional structure and functional groups on the surface of the nano magnetic beads, making the nano magnetic beads better compatible with the water sample; the nano magnetic bead washing solution is used to wash away the impurities, that is, non-target substances, adsorbed or remaining on the surface of the nano magnetic beads in the water sample, to prevent the impurities from entering the subsequent eluent and affecting the final measurement result, causing measurement errors.

[0078] Specifically, the X-axis moving device 21 includes an X-axis driving member 211, an X-axis screw 212, an X-axis sliding nut 213, an X-axis connecting plate 214, and an X-axis guide rail 215;

[0079] The X-axis screw 212 is arranged on the sample stage 2 along the X-axis direction, and both ends of the X-axis screw 212 are rotatably connected to the sample stage 2; one end of the X-axis screw 212 is in transmission connection with the driving end of the X-axis driving member 211; the X-axis sliding nut 213 is threadedly assembled with the X-axis screw 212; one side of the X-axis connecting plate 214 is connected to the X-axis sliding nut 213; the other side of the X-axis connecting plate 214 is connected to the support 5;

[0080] The X-axis guide rail 215 is arranged on the sample stage 2 along the X-axis direction, and an X-axis slider 216 is provided on the other side of the X-axis connecting plate 214, and the X-axis slider 216 is slidably assembled with the X-axis guide rail 215;

[0081] The X-axis driving member 211 is arranged on the sample stage 2 and drives the X-axis screw 212 to rotate about its own axis, so that the X-axis sliding nut 213 and the X-axis connecting plate 214 move along the X-axis direction.

[0082] The transmission principle of the X-axis moving device 21 can also refer to the ball screw, which converts the rotational motion into a linear motion. The spiral groove on the X-axis screw 212 guides the X-axis sliding nut 213 to move axially. Due to the limitation of the X-axis guide rail 215 and the X-axis slider 216, the X-axis sliding nut 213 moves axially along the X-axis screw 212.

[0083] Adopting the above transmission structure can transmit torque with higher efficiency, and has lower friction resistance, higher precision and repeat positioning accuracy, and can withstand larger axial and radial loads, and has a longer service life.

[0084] In a specific embodiment of the present utility model, the X-axis moving device 21 is disposed at the bottom of the sample stage 2. The sample stage 2 is provided with an avoidance area along the X-axis direction. On the other side of the X-axis connecting plate 214, there is an X-axis connecting column. The X-axis connecting column passes through the avoidance area and is connected to the support 5. The X-axis driving member 211 is disposed on one side of the X-axis screw 212. The driving end of the X-axis driving member 211 and the outer periphery of one end of the X-axis screw 212 are sleeved with a second transmission belt. The driving end of the X-axis driving member 211 drives the X-axis screw 212 to rotate about its own axis through the second transmission belt. With the above structure, the space can be reasonably utilized, making the pretreatment instrument more compact and small.

[0085] Specifically, the sample stage 2 is further provided with a heating device 22, and the sample rack 3 is mounted on the outer periphery of the heating device 22; the heating device 22 is disposed at the bottom of the sample stage 2, and the heating end of the heating device 22 passes through the sample stage 2; the elution tube 43 is inserted into the heating end of the heating device 22.

[0086] The heating device 22 can refer to the existing test tube heating device. The heating end of the heating device 22 is provided with a heating hole, and the elution tube 43 is inserted into the heating hole. With the above structure, in cooperation with the air blowing device 8, all or part of the volatile solvents in the eluent can be quickly volatilized, realizing the concentration or drying of the eluent.

[0087] The bottom of the chassis 1 is provided with heat dissipation holes, which are convenient for discharging the hot air generated by the heating device 22.

[0088] In a specific embodiment of the present utility model, the pretreatment process includes: removing the packaging films of the sample tank 42, the magnetic bead treatment tube group and the elution tube 43, pouring a specified amount of water sample into the sample tank 42, adding an appropriate amount of nano magnetic beads into the first treatment tube 44, and moving the stirring sleeve 73 up and down to fully stir for a specified time to activate the three-dimensional structure and functional groups on the surface of the nano magnetic beads;

[0089] Then, the magnetic attraction rod 63 cooperates with the stirring sleeve 73 to transfer the nano magnetic beads to the second treatment tube 45; the stirring sleeve 73 moves up and down to fully stir for a specified time to balance the three-dimensional structure and functional groups on the surface of the nano magnetic beads;

[0090] Subsequently, the magnetic attraction rod 63 cooperates with the stirring sleeve 73 to transfer the nano magnetic beads to the sample tank 42; the stirring sleeve 73 reciprocates left and right to stir to fully stir for a specified time to make the nano magnetic beads fully mixed with the sample, and the target substance is fully adsorbed on the surface of the nano magnetic beads through physical or / and chemical binding forces;

[0091] Immediately afterwards, the magnetic attraction rod 63 cooperates with the stirring sleeve 73 to transfer the nano magnetic beads to the third treatment tube 46, and the stirring sleeve 73 moves up and down to fully stir for a specified time to wash away impurities;

[0092] Again, through the cooperation of the magnetic attraction rod 63 and the stirring sleeve 73, the nano magnetic beads are transferred to the elution tube 43. The stirring sleeve 73 moves up and down again to fully stir for a specified duration, so that the eluent elutes the target substance from the surface of the nano magnetic beads. Finally, the magnetic attraction rod 63 and the stirring sleeve 73 cooperate to suck the nano magnetic beads away from the eluent, leaving the target substance in the eluent.

[0093] The air injection needle 84 is inserted into the elution tube 43 at a specified depth, the heating hole of the heating device 22 is maintained at a specified temperature, and the blower 83 generates an air flow to blow off all or part of the volatile solvent in the eluent, realizing concentration or drying. Before measurement, an appropriate amount of reconstitution solution can be added for reconstitution.

[0094] Preferably, the chassis 1 is provided with an air filter 11. The air filter 11 is specifically a high-efficiency air purification filter. In a preferred embodiment, the chassis 1 has two air filters 11, one is arranged on the left side and the other is arranged on the right side of the chassis 1. One air filter 11 filters the outside air to provide clean air in the sample chamber 10, avoiding the entry of external aerosols and causing sample contamination; the other air filter 11 filters the air in the sample chamber 10, intercepting the aerosols generated in the sample chamber 10 on the air filter membrane to avoid discharging to the outside and polluting the external environment.

[0095] Specifically, it further includes a control device 9. The control device 9 includes a control module 91 and a control panel 92. The control module 91 is arranged on the top of the chassis 1. The control module 91 is electrically connected to the magnetic attraction device 6, the stirring device 7 and the air blowing device 8 respectively, and is used to control the magnetic attraction device 6, the stirring device 7 and the air blowing device 8;

[0096] The control panel 92 is arranged on the outside of the chassis 1. The control panel 92 is electrically connected to the control device 9.

[0097] With the above structure, the staff can input instructions through the control panel 92 to make the control device 9 start the magnetic attraction device 6, the stirring device 7 and the air blowing device 8 according to the program, and automatically perform the pretreatment, realizing the automation of sample pretreatment.

[0098] The technical principle of the present invention has been described above in combination with specific embodiments. These descriptions are only for explaining the principle of the present invention and cannot be interpreted in any way as a limitation on the protection scope of the present invention. Based on the explanations herein, those skilled in the art can think of other specific embodiments of the present invention without creative labor, and these equivalent variations or substitutions are all included within the scope defined by the claims of this application.

Claims

1. A pre-treatment instrument based on nanomagnetic bead extraction method, characterized in that: It includes a chassis, a sample loading platform, a sample rack, a test tube, a support, a magnetic suction device, a stirring device and an air blowing device; The chassis is provided with a sample compartment, the sample compartment is provided with a sample loading platform, and the sample loading platform is provided with an X-axis moving device; the transmission end of the X-axis moving device is transmission-connected with the sample loading platform to drive the sample loading platform to move along the X-axis direction; The sample rack is placed on the sample loading platform, and the detection test tube is fixed in the sample rack; the detection test tube includes a parallel plate, a sample slot, an elution tube and a magnetic bead processing tube group; the sample slot, the magnetic bead processing tube group and the elution tube are sequentially arranged on the parallel plate along the X-axis direction; the sample slot is used to place water samples; the magnetic bead processing tube group includes two or more magnetic bead processing tubes, and the magnetic bead processing tubes are respectively filled with reagents required for processing the magnetic beads before and after adsorbing the target, and the elution tube is encapsulated with an eluent; The magnetic attraction device absorbs or releases nanomagnetic beads through changes in magnetic force, so that the nanomagnetic beads enter the magnetic bead processing tube, the sample tank and the elution tube in sequence; the stirring device is used to stir the liquid in the sample tank, the magnetic bead processing tube or the elution tube; the blowing device is arranged on the support, and is used to blow dry or partially evaporate the eluent.

2. A pre-treatment instrument based on nanomagnetic bead extraction method according to claim 1, characterized in that: The magnetic attraction device comprises a magnetic attraction motion module, a magnetic attraction arm and a magnetic attraction rod; the magnetic attraction motion module is arranged on a support; the magnetic attraction rod is arranged on the magnetic attraction arm; The stirring device comprises a stirring motion module, a stirring arm and a stirring sleeve; the stirring motion module is arranged on a support, and the stirring motion module is located on one side of the magnetic attraction motion module; the stirring sleeve is arranged on the stirring arm; the driving end of the stirring motion module is connected to the side of the stirring arm, and the stirring arm is located directly below the magnetic attraction arm, and the stirring sleeve is located directly below the projection of the magnetic attraction rod in the vertical direction; The stirring motion module drives the stirring arm and the stirring sleeve to move along the Z-axis direction; the driving end of the magnetic attraction motion module is connected to the magnetic attraction arm to drive the magnetic attraction arm and the magnetic attraction rod to move along the Z-axis direction, and to insert the magnetic attraction rod into the stirring sleeve or leave the stirring sleeve.

3. A pre-treatment instrument based on nanomagnetic bead extraction method according to claim 2, characterized in that: The stirring arm is provided with a slot along the Y-axis direction, the slot is located at one end of the stirring arm away from the stirring motion module, and a clearance opening is provided below the slot; the top of the stirring arm is provided with an insertion interface connected to the slot; The stirring sleeve comprises a stirring sleeve beam, a stirring sleeve handle and a stirring sleeve pipe; the stirring sleeve handle is connected to one end of the stirring sleeve beam, the stirring sleeve pipe is connected to the stirring sleeve beam, and the position of the stirring sleeve pipe is adapted to the magnetic rod; the stirring sleeve beam is inserted into the slot, and the stirring sleeve pipe passes through the slot through the avoidance opening.

4. A pre-treatment instrument based on nanomagnetic bead extraction method according to claim 2, characterized in that: The blowing device includes a blowing motion module, a blowing arm, a blower and a blowing needle; the blowing motion module is arranged on a support, and the blowing motion module is located on one side of the magnetic attraction motion module; the blowing needles are respectively arranged on the blowing arms, and the air outlet of the blower is connected to the air inlet of the blowing needle; the driving end of the blowing motion module is connected to the blowing arm to drive the blowing arm and the blowing needle to move along the Z-axis direction; the blowing needle is located above the inspection test tube.

5. A pre-treatment instrument based on nanomagnetic bead extraction method according to claim 4, characterized in that: The magnetic attraction motion module, the stirring motion module and the air blowing motion module have the same structure, including a Z-axis driving member, a Z-axis screw, a Z-axis sliding nut, a Z-axis connecting plate and a Z-axis guide rail; The Z-axis screw is arranged on the support along the Z-axis direction, one end of the Z-axis screw passes through the support and is drivingly connected to the driving end of the Z-axis driving member; the other end of the Z-axis screw is rotatably connected to the support; the Z-axis sliding nut is threadedly assembled with the Z-axis screw; one side of the Z-axis connecting plate is connected to the Z-axis screw, and the other side of the Z-axis connecting plate extends forward with a connecting arm, and the connecting arm is connected to the magnetic suction arm, the stirring arm or the blowing arm; The Z-axis guide rail is arranged on the support along the Z-axis direction, and a Z-axis slider is also provided on the other side of the Z-axis connecting plate, and the Z-axis slider is slidably assembled with the Z-axis guide rail; the Z-axis driving component is arranged on the support, and drives the Z-axis screw to rotate with its own axis as the rotation axis, so that the Z-axis sliding nut and the Z-axis connecting plate rise or fall.

6. A pre-treatment instrument based on nanomagnetic bead extraction method according to claim 1, characterized in that: The magnetic bead processing tube set comprises a first processing tube, a second processing tube and a third processing tube, wherein the first processing tube, the second processing tube and the third processing tube are sequentially arranged on the parallel plate along the X-axis direction; The first processing tube is encapsulated with a nano-magnetic bead activation solution; the second processing tube is encapsulated with a nano-magnetic bead balancing solution; and the third processing tube is encapsulated with a nano-magnetic bead washing solution.

7. A pre-treatment instrument based on nanomagnetic bead extraction method according to claim 1, characterized in that: The X-axis moving device includes an X-axis driving member, an X-axis screw, an X-axis sliding nut, an X-axis connecting plate and an X-axis guide rail; The X-axis screw is arranged on the sample stage along the X-axis direction, and both ends of the X-axis screw are rotatably connected to the sample stage respectively; one end of the X-axis screw is transmission-connected to the driving end of the X-axis driving member; the X-axis sliding nut is threadedly assembled with the X-axis screw; one side of the X-axis connecting plate is connected to the X-axis sliding nut; the other side of the X-axis connecting plate is connected to the support; The X-axis guide rail is arranged on the sample loading platform along the X-axis direction, and an X-axis slider is arranged on the other side of the X-axis connecting plate, and the X-axis slider is slidably assembled with the X-axis guide rail; The X-axis driving member is arranged on the sample loading platform, and drives the X-axis screw to rotate with its own axis as the rotation axis, so that the X-axis sliding nut and the X-axis connecting plate move along the X-axis direction.

8. A pre-treatment instrument based on nanomagnetic bead extraction method according to claim 1, characterized in that: The sample loading platform is also provided with a heating device, and the sample rack is mounted on the periphery of the heating device; the heating device is arranged at the bottom of the sample loading platform, and the heating end of the heating device passes through the sample loading platform; the elution tube is plugged into the heating end of the heating device.

9. A pre-treatment instrument based on nanomagnetic bead extraction method according to claim 1, characterized in that: The chassis is provided with an air filter.

10. A pre-treatment instrument based on nanomagnetic bead extraction method according to claim 1, characterized in that: It also includes a control device, which includes a control module and a control panel. The control module is arranged on the top of the chassis, and the control module is electrically connected to the magnetic attraction device, the stirring device and the blowing device, respectively, and is used to control the magnetic attraction device, the stirring device and the blowing device; The control panel is arranged on the outside of the chassis, and the control panel is electrically connected to the control device.

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