Serum exosome removing device

Abstract

The utility model relates to the technical field of biomedical engineering, in particular to a serum exosome removing device which comprises a centrifugal barrel and a sample injection pump, a sample injection pipe is fixedly installed at the lower end of the sample injection pump in a penetrating mode, a filtering assembly is fixedly connected to the left portion of the upper end of the sample injection pipe in a penetrating mode, and a conveying pipe is fixedly installed at the upper end of the sample injection pump in a penetrating mode. The right portion of the lower end of the conveying pipe penetrates through the upper end of the centrifugal barrel and extends into the centrifugal barrel, a centrifugal device is installed in the middle of the upper end of the centrifugal barrel in a penetrating mode, and a collecting assembly is fixedly connected to the right portion of the lower end of the centrifugal barrel in a penetrating mode. According to the serum exosome removal device, through preliminary filtration of the ultrafiltration membrane, centrifugal treatment of the centrifugal device and specific capture of the immunoaffinity chromatography column, efficient separation of serum exosomes can be achieved, the purity and accuracy of separation are improved, the whole process is relatively mild, and the operation is simple. The damage to the structure and the function of the serum exosome is reduced, and the biological activity of the exosome is favorably kept.

Description

Technical Field

[0001] The utility model relates to the technical field of biomedical engineering, and particularly relates to a serum exosome removal device. Background Art

[0002] In the fields of biomedical research and clinical diagnosis, the research on serum exosomes is indeed of great significance. As a kind of tiny vesicles secreted by cells (usually with a diameter between 30 and 150 nanometers), exosomes contain rich biological information inside, including proteins, nucleic acids (such as mRNA, miRNA, and lncRNA), and lipids, etc. These information play crucial roles in cell - cell communication, the occurrence and development of diseases, as well as diagnosis and treatment. However, the traditional methods for removing and separating serum exosomes still have the problem of low separation purity, which greatly limits the application of exosomes in scientific research and clinical practice. Therefore, we introduce a serum exosome removal device. Content of the Utility Model

[0003] The main purpose of the utility model is to provide a serum exosome removal device, which can effectively solve the problems in the background art.

[0004] To achieve the above - mentioned purpose, the technical solution adopted by the utility model is as follows:

[0005] A serum exosome removal device includes a centrifuge bucket and a sample injection pump. The lower end of the sample injection pump is fixedly installed with a sample injection pipe in an inserted manner. The left part of the upper end of the sample injection pipe is fixedly connected with a filtering component in an inserted manner. The upper end of the filtering component is sleeved with a sealing cover. The upper end of the sample injection pump is fixedly installed with a delivery pipe. The lower right part of the delivery pipe penetrates through the upper end of the centrifuge bucket and extends into the centrifuge bucket. The middle part of the upper end of the centrifuge bucket is installed with a centrifugation device in an inserted manner. The lower right part of the centrifuge bucket is fixedly connected with a collection component;

[0006] The filtering component includes a sample storage bucket. The inner wall surface of the sample storage bucket is fixedly connected with a fixed frame. The inner wall surface of the fixed frame is fixedly connected with an ultrafiltration membrane. The lower end of the sample storage bucket is fixedly connected with the upper end of the sample injection pipe in an inserted manner.

[0007] Preferably, the lower end of the sealing cover is sleeved on the upper end of the sample storage bucket.

[0008] By adopting the above - mentioned technical solution: providing a relatively closed environment for the sample helps to maintain the chemical and physical properties of the serum and ensure the accuracy of subsequent separation operations.

[0009] Preferably, the centrifugation device includes a centrifugation motor, the output end of the centrifugation motor is fixedly installed with a centrifuge tube, several centrifugation holes penetrating through the inside and outside are formed on the outer surface of the centrifuge tube, the lower end of the centrifugation motor is fixedly connected to the upper end of the centrifugation barrel, and the output end of the centrifugation motor penetrates through the upper end of the centrifugation barrel and extends into the centrifugation barrel.

[0010] By adopting the above technical solution: the existence of the centrifugation holes promotes the material exchange between the inside and outside of the centrifuge tube, helps the exosomes and other components in the serum to be better separated, and also facilitates the discharge of the separated substances.

[0011] Preferably, the lower end of the centrifuge tube is movably connected to the lower inner wall surface of the centrifugation barrel through a bearing, and several of the centrifugation holes are distributed in a circular array centered on the centrifuge tube.

[0012] By adopting the above technical solution: several centrifugation holes are distributed in a circular array centered on the centrifuge tube, making the centrifugal force received by the serum during centrifugation more uniform, and helping the exosomes to be separated more thoroughly and evenly from other components.

[0013] Preferably, the delivery pipe is provided as a flexible pipe, and the right part of the lower end of the delivery pipe is movably connected to the upper end of the centrifuge tube in an inserted manner.

[0014] By adopting the above technical solution: the delivery pipe is provided as a flexible pipe, which has good flexibility and bendability, is convenient for being movably connected to the upper end of the centrifuge tube in an inserted manner, and reduces the installation difficulty and the limitation on the space layout.

[0015] Preferably, the collection assembly includes a fixed pipe, an immunoaffinity chromatography column is fixedly connected to the lower end of the fixed pipe in an inserted manner, a connecting pipe is fixedly connected to the lower end of the immunoaffinity chromatography column in an inserted manner, a collection pipe is fixedly connected to the lower end of the connecting pipe in an inserted manner, and the upper end of the fixed pipe is fixedly connected to the lower end of the centrifugation barrel in an inserted manner.

[0016] By adopting the above technical solution: through the sequential connection of the fixed pipe, the immunoaffinity chromatography column, the connecting pipe and the collection pipe, a complete and smooth channel is formed, which can effectively guide the serum components after centrifugation treatment to the corresponding positions in sequence, realizing the specific capture of exosomes and the accurate collection of other components.

[0017] Compared with the prior art, the present utility model has the following beneficial effects:

[0018] 1. In the present utility model, through the preliminary filtration of the ultrafiltration membrane, the centrifugation treatment of the centrifugation device and the specific capture of the immunoaffinity chromatography column, the efficient separation of serum exosomes can be realized, the separation purity and accuracy are improved, the whole process is relatively mild, the damage to the structure and function of serum exosomes is reduced, and it helps to maintain the biological activity of exosomes;

[0019] 2. In the present utility model, the sampling pump can accurately control the input speed and flow rate of the serum sample, ensuring the stability and repeatability of the entire separation process. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a schematic diagram of the overall structure of a serum exosome removal device of the present utility model;

[0021] Figure 2 It is a schematic diagram of the overall structure of the filtering component of a serum exosome removal device of the present utility model;

[0022] Figure 3 It is a schematic diagram of the overall structure of the centrifugal device of a serum exosome removal device of the present utility model;

[0023] Figure 4 It is a schematic diagram of the overall structure of the collection component of a serum exosome removal device of the present utility model.

[0024] In the figure: 1, centrifugal barrel; 2, sampling pump; 3, sampling tube; 4, filtering component; 5, sealing cover; 6, delivery tube; 7, centrifugal device; 8, collection component; 41, sample storage barrel; 42, fixing frame; 43, ultrafiltration membrane; 71, centrifugal motor; 72, centrifuge tube; 73, centrifugal hole; 81, fixing tube; 82, immunoaffinity chromatography column; 83, connecting tube; 84, collection tube. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0025] In order to make the technical means, creative features, achieved purposes and effects of the present utility model easy to understand, the present utility model will be further described below in conjunction with specific embodiments.

[0026] In the description of the present utility model, it should be noted that the orientation or positional relationship indicated by the terms "upper", "lower", "inner", "outer", "front end", "rear end", "both ends", "one end", "the other end", etc. is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the present utility model. In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be construed as indicating or implying relative importance.

[0027] In the description of the present utility model, it should be noted that unless otherwise clearly specified and defined, terms such as "installation", "provided with", "connection", etc. should be understood in a broad sense. For example, "connection" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific situations.

[0028] Please refer to Figures 1-4 , the present utility model provides a technical solution:

[0029] A serum exosome removal device, comprising a centrifugation barrel 1 and a sample injection pump 2. The lower end of the sample injection pump 2 is fixedly installed with a sample injection tube 3 in an inserted manner. The left part of the upper end of the sample injection tube 3 is fixedly connected with a filtering assembly 4 in an inserted manner. The upper end of the filtering assembly 4 is sleeved with a sealing cover 5. The upper end of the sample injection pump 2 is fixedly installed with a conveying tube 6 in an inserted manner. The lower right part of the conveying tube 6 penetrates through the upper end of the centrifugation barrel 1 and extends into the centrifugation barrel 1. The middle part of the upper end of the centrifugation barrel 1 is inserted with a centrifugation device 7. The lower right part of the centrifugation barrel 1 is fixedly connected with a collection assembly 8 in an inserted manner.

[0030] In this embodiment, the filtering assembly 4 includes a sample storage barrel 41. The inner wall surface of the sample storage barrel 41 is fixedly connected with a fixed frame 42. The inner wall surface of the fixed frame 42 is fixedly connected with an ultrafiltration membrane 43. The lower end of the sample storage barrel 41 is fixedly connected with the upper end of the sample injection tube 3 in an inserted manner. The lower end of the sealing cover 5 is sleeved on the upper end of the sample storage barrel 41. The centrifugation device 7 includes a centrifugation motor 71. The output end of the centrifugation motor 71 is fixedly installed with a centrifugation tube 72. A plurality of centrifugation holes 73 that penetrate through the inside and outside are formed on the outer surface of the centrifugation tube 72. The lower end of the centrifugation motor 71 is fixedly connected with the upper end of the centrifugation barrel 1. The output end of the centrifugation motor 71 penetrates through the upper end of the centrifugation barrel 1 and extends into the centrifugation barrel 1. The lower end of the centrifugation tube 72 is movably connected with the lower inner wall surface of the centrifugation barrel 1 through a bearing. The plurality of centrifugation holes 73 are distributed in a circular array centered on the centrifugation tube 72. The conveying tube 6 is set as a flexible tube. The lower right part of the conveying tube 6 is movably connected with the upper end of the centrifugation tube 72 in an inserted manner.

[0031] Through the above solution: First, inject the serum sample to be processed into the sample storage bucket 41, cover the sealing cover 5 to ensure that the sample storage bucket 41 is in a sealed state. In the sample storage bucket 41, the serum contacts the ultrafiltration membrane 43 in the fixing frame 42. Due to the pore size characteristics of the ultrafiltration membrane 43, it can intercept the exosomes in the serum while allowing small molecule substances and other components in the serum to pass through, thus achieving preliminary separation. Then, start the injection pump 2. The injection pump 2 draws the serum that has been preliminarily treated by ultrafiltration through the injection tube 3. Under the action of the injection pump 2, the serum is transported along the delivery tube 6 to the centrifuge tube 72 in the centrifuge bucket 1. Then, pull out the delivery tube 6 from the centrifuge tube 72. At this time, start the centrifuge motor 71. The output end of the centrifuge motor 71 drives the centrifuge tube 72 to rotate at a high speed. Due to the action of centrifugal force, different components in the serum will be stratified according to the differences in their density and mass. The exosomes, due to their relatively large mass and density, will approach the inner wall of the centrifuge tube 72. A number of centrifugal holes 73 are arranged in a circular array on the outer surface of the centrifuge tube 72, enabling the serum to carry out mass exchange and further separation between the centrifuge tube 72 and the centrifuge bucket 1 during the centrifugation process.

[0032] In this embodiment, the collection assembly 8 includes a fixed tube 81. The lower end of the fixed tube 81 is fixedly connected in an inserted manner with an immunoaffinity chromatography column 82. The lower end of the immunoaffinity chromatography column 82 is fixedly connected in an inserted manner with a connecting tube 83. The lower end of the connecting tube 83 is fixedly connected in an inserted manner with a collection tube 84. The upper end of the fixed tube 81 is fixedly connected in an inserted manner with the lower end of the centrifuge bucket 1.

[0033] Through the above solution: The serum after centrifugation treatment flows out from the lower end of the centrifuge bucket 1 and enters the immunoaffinity chromatography column 82 through the fixed tube 81 fixedly connected to it in an inserted manner. The immunoaffinity chromatography column 82 is filled with antibodies that can specifically recognize and bind to the surface markers of exosomes. When the serum flows through the immunoaffinity chromatography column 82, the exosomes are intercepted and captured in the chromatography column due to specific binding to the antibodies. The serum components that do not bind to the antibodies continue to flow downward, pass through the connecting tube 83 fixedly connected to the lower end of the immunoaffinity chromatography column 82 in an inserted manner, and finally enter the collection tube 84 fixedly connected to it in an inserted manner, thereby achieving the collection of the serum after removing exosomes.

[0034] It should be noted that the present utility model is a serum exosome removal device. During use, first, pour the serum sample to be processed into the sample storage bucket 41, and then cover the sealing cover 5 on the sample storage bucket 41. The sealing cover 5 ensures that the sample storage bucket 41 is in a sealed state to prevent the sample from being contaminated. The serum in the sample storage bucket 41 contacts the ultrafiltration membrane 43 in the fixed frame 42 under the action of gravity. The ultrafiltration membrane 43 has a specific pore size, which can intercept exosomes in the serum and allow other small molecule substances and solutes in the serum to pass through, initially realizing the separation of exosomes from other components. Start the injection pump 2, and the injection pump 2 pumps and pressurizes the serum that has been preliminarily treated by the ultrafiltration membrane 43 through the injection pipe 3, and makes it flow along the delivery pipe 6 into the centrifuge tube 72 in the centrifuge bucket 1. Then, pull out the delivery pipe 6 from the centrifuge tube 72. Start the centrifuge motor 71 to drive the centrifuge tube 72 to rotate at a high speed. Due to the action of centrifugal force, the components in the serum are stratified according to their different densities and masses. Under the action of centrifugal force, exosomes approach the inner wall of the centrifuge tube 72, while other components are distributed in different positions. A number of centrifugal holes 73 opened on the outer surface of the centrifuge tube 72 enable the centrifuged serum to exchange substances with the space in the centrifuge bucket 1. The serum after centrifugation enters the immunoaffinity chromatography column 82 from the lower part of the centrifuge bucket 1 through the fixed pipe 81. The immunoaffinity chromatography column 82 is filled with antibodies that specifically recognize the surface markers of exosomes. Exosomes can bind to the antibodies and be specifically captured, while other components enter the collection tube 84 through the connecting pipe 83, completing the removal and separation of serum exosomes. Finally, the serum after removing exosomes is collected in the collection tube 84, and the captured exosomes remain in the immunoaffinity chromatography column 82 and can be separately collected and processed through corresponding operations.

[0035] The above shows and describes the basic principles, main features and advantages of the present utility model. Those skilled in the art of this industry should understand that the present utility model is not limited by the above embodiments. What is described in the above embodiments and the specification only illustrates the principle of the present utility model. Without departing from the spirit and scope of the present utility model, the present utility model will have various changes and improvements, and these changes and improvements all fall within the scope of the present utility model claimed. The scope of protection claimed by the present utility model is defined by the appended claims and their equivalents.

Claims

1. A serum exosome removal device, comprising a centrifugal bucket (1) and a sample injection pump (2), characterized in that: The lower end of the sample injection pump (2) is inserted and fixedly installed with a sample injection tube (3), the upper left part of the sample injection tube (3) is inserted and fixedly connected with a filter assembly (4), the upper end of the filter assembly (4) is sleeved with a sealing cover (5), the upper end of the sample injection pump (2) is inserted and fixedly installed with a delivery tube (6), the lower right part of the delivery tube (6) passes through the upper end of the centrifugal bucket (1) and extends into the centrifugal bucket (1), the middle part of the upper end of the centrifugal bucket (1) is inserted and installed with a centrifugal device (7), and the lower right part of the centrifugal bucket (1) is inserted and fixedly connected with a collection assembly (8); The filtering assembly (4) comprises a sample storage barrel (41), the inner wall surface of the sample storage barrel (41) is fixedly connected to a fixing frame (42), the inner wall surface of the fixing frame (42) is fixedly connected to an ultrafiltration membrane (43), and the lower end of the sample storage barrel (41) is fixedly connected to the upper end of the sample injection tube (3) through insertion.

2. A serum exosome removal device according to claim 1, characterized in that: The lower end of the sealing cover (5) is sleeved on the upper end of the sample storage barrel (41).

3. A serum exosome removal device according to claim 1, characterized in that: The centrifugal device (7) comprises a centrifugal motor (71), a centrifugal tube (72) is fixedly mounted on the output end of the centrifugal motor (71), a plurality of centrifugal holes (73) passing through the outer surface of the centrifugal tube (72), the lower end of the centrifugal motor (71) is fixedly connected to the upper end of the centrifugal barrel (1), and the output end of the centrifugal motor (71) passes through the upper end of the centrifugal barrel (1) and extends into the centrifugal barrel (1).

4. A serum exosome removal device according to claim 3, characterized in that: The lower end of the centrifuge tube (72) is movably connected to the lower inner wall surface of the centrifuge barrel (1) via a bearing, and a plurality of centrifuge holes (73) are distributed in a central annular array of the centrifuge tube (72).

5. A serum exosome removal device according to claim 1, characterized in that: The delivery pipe (6) is configured as a hose, and the right portion of the lower end of the delivery pipe (6) is movably connected to the upper end of the centrifuge tube (72) by interlacing.

6. A serum exosome removal device according to claim 1, characterized in that: The collecting assembly (8) comprises a fixed tube (81), the lower end of the fixed tube (81) is inserted and fixedly connected with an immunoaffinity chromatography column (82), the lower end of the immunoaffinity chromatography column (82) is inserted and fixedly connected with a connecting tube (83), the lower end of the connecting tube (83) is inserted and fixedly connected with a collecting tube (84), and the upper end of the fixed tube (81) is inserted and fixedly connected with the lower end of the centrifugal bucket (1).

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