Absorbance measuring device capable of penetrating through sample to be measured for multiple times

Abstract

The utility model relates to an absorbance measuring device capable of penetrating through a sample to be measured for multiple times. The problem that a to-be-detected solution with low concentration and small volume is difficult to directly measure through light transmission detection in the prior art is solved. The device comprises a to-be-detected liquid sample and a blank control sample, the to-be-detected liquid sample and the blank control sample are loaded in a sample loading mechanism, and a light source for emitting penetrating light and a photoelectric sensor for receiving light are arranged on the outer side of the sample loading mechanism respectively. And a plurality of optical fibers or reflecting mirror groups for receiving and reflecting light penetrating through the liquid sample to be detected are arranged on two sides of the sample loading mechanism. The device has the advantages that the effective optical path penetrating through the liquid sample to be detected is increased by penetrating through the liquid sample to be detected for multiple times, so that the corresponding absorbance value An is measured and is easily detected by detection equipment, the detection lower limit can be reduced to 1 / N of the detection lower limit in the prior art, and the detection range of the equipment is greatly expanded.

Description

Technical Field

[0001] The utility model relates to the technical field of analytical instruments, and particularly relates to an absorbance measurement device for penetrating a sample to be measured multiple times. Background Art

[0002] Absorbance analysis is a common spectroscopic analysis technique, and its principle is to perform quantitative or qualitative analysis by measuring the absorbance of a substance to light of a specific wavelength. Absorbance measurement has important application values in fields such as chemistry, biomedicine, environmental science, pharmaceutical and food industries.

[0003] In the prior art, most absorbance measurement methods obtain the absorbance of a sample by measuring the parameters of the transmitted light after passing through the sample once. For example, a Chinese patent document discloses a spectrophotometer with a dual sampling mode [CN200980147668.6], which introduces a dual-mode method and device for selectively measuring a sample in a vessel or a sample (5, 5') in a surface tension mode held between two opposite bases (A', A"). In any configuration, the above mode also includes an optical path from a source system, through a small-volume or large-volume sample, to a spectrometer-based system. For any given wavelength, the above system enables a user to measure a sample with an absorbance range from about 0.005 to about 2.0 absorbance units.

[0004] There are also a small number of technical solutions that use the sample to be penetrated twice to measure the absorbance of the sample. For example, a Chinese patent document discloses an optical fiber reflection type micro-nano system spectrophotometer and its application [CN200910236148.4]. The spectrophotometer provided by this invention includes the following components: an excitation light source, an emission optical fiber with one end connected to the excitation light source, a spectrometer, and a receiving optical fiber with one end connected to the spectrometer; on this basis, the other ends of the emission optical fiber and the receiving optical fiber are combined into a combined interface; a light reflection device with a light reflection surface is arranged on the opposite side of the end surface of the combined interface; the gap formed between the end surface of the combined interface and the light reflection surface is a droplet accommodation gap for accommodating the sample to be measured. By adopting the above technical solution, the sample to be measured only needs to be compressed instead of being stretched into a liquid column to achieve the required measurement optical path. Compared with the transmission single-fold optical path sample absorption scheme adopted in the prior art, this method has better measurement accuracy and repeatability, can require less sample usage, and the structure of the droplet optical path maintaining part is simplified, and the cost of the instrument is lower.

[0005] It can be seen that in the prior art, there is at most a technical solution that obtains the absorbance of a sample to be measured by passing through the sample twice. Due to the short optical path and the corresponding small absorbance, it is impossible to directly measure a sample solution to be measured with a low concentration and a small volume. According to the content of the basic law of the absorbance analysis method - Lambert-Beer's law, A = lg(1 / T) = K * b * c, where A is the absorbance, T is the transmittance ratio, that is, the transmittance, which is the ratio of the intensity of the outgoing light I1 to the intensity of the incident light I0. K is the molar absorptivity, which is related to the properties of the absorbing substance and the wavelength λ of the incident light, c is the concentration of the absorbing substance in mol / L, and b is the thickness of the absorption layer, with the unit of cm. It can be seen from Lambert-Beer's law that when the absorbance of a single sample is small and difficult to detect, passing through the sample multiple times is equivalent to increasing the thickness of the absorption layer, resulting in a significant decrease in the intensity of the outgoing light compared to the intensity of the incident light, that is, increasing the value of the absorbance, which is beneficial to the accurate measurement of dilute solution samples. Summary of the Invention

[0006] The purpose of the present utility model is to address the above problems and provide an absorbance measurement device that penetrates a sample to be measured multiple times.

[0007] To achieve the above purpose, the present utility model adopts the following technical solutions: An absorbance measurement device that penetrates a sample to be measured multiple times includes a liquid sample to be detected and a blank control sample. The liquid sample to be detected and the blank control sample are loaded in a sample loading mechanism. A light source for emitting penetrating light and a photoelectric sensor for receiving light are respectively provided outside the sample loading mechanism, and a plurality of optical fibers or mirror groups for receiving the light transmitted through the liquid sample to be detected and reflecting it are provided on both sides of the sample loading mechanism.

[0008] In the above absorbance measurement device that penetrates a sample to be measured multiple times, the sample loading mechanism is a cuvette, and the liquid sample to be detected and the blank control sample are loaded in the cuvette.

[0009] In the above absorbance measurement device that penetrates a sample to be measured multiple times, a light source is provided at one end of the cuvette, and a photoelectric sensor for receiving the light that has been transmitted through the liquid sample to be detected after being transmitted through the optical fiber N times is provided at one end. The optical fibers or mirror groups are arranged in a staggered manner on both sides of the cuvette and receive and reflect the light emitted by the light source.

[0010] In the above absorbance measurement device that penetrates a sample to be measured multiple times, the sample loading mechanism is a closed space, and the liquid sample to be detected and the blank control sample are loaded in the closed space.

[0011] In the above absorbance measurement device for repeatedly penetrating a sample to be measured, a light source is provided at one end of the enclosed space, and a photoelectric sensor for receiving the light that has been transmitted N times through the optical fiber and passed through the liquid sample to be detected is provided at one end. The optical fiber or the mirror group is arranged at the two sides of the enclosed space in a misaligned manner to receive and reflect the light emitted by the light source.

[0012] In the above absorbance measurement device for repeatedly penetrating a sample to be measured, the sample loading mechanism is the first optical fiber head and the second optical fiber head, and the liquid sample to be detected and the blank control sample are arranged between the first optical fiber head and the second optical fiber head.

[0013] In the above absorbance measurement device for repeatedly penetrating a sample to be measured, a light source is provided at one end of the first optical fiber head and the second optical fiber head, and a photoelectric sensor for receiving the light that has been transmitted N times through the optical fiber and passed through the liquid sample to be detected is provided at one end. The optical fiber or the mirror group is arranged at the two sides of the first optical fiber head and the second optical fiber head in a misaligned manner to receive and reflect the light emitted by the light source.

[0014] In the above absorbance measurement device for repeatedly penetrating a sample to be measured, the sample loading mechanism is the first optical fiber head group and the second optical fiber head group, and the liquid sample to be detected and the blank control sample are arranged between the first optical fiber head group and the second optical fiber head group.

[0015] In the above absorbance measurement device for repeatedly penetrating a sample to be measured, a light source is provided at one end of the first optical fiber head group and the second optical fiber head group, and a photoelectric sensor for receiving the light that has been transmitted N times through the optical fiber and passed through the liquid sample to be detected is provided at one end. The optical fiber or the mirror group is arranged at the two sides of the first optical fiber head group and the second optical fiber head group in a misaligned manner to receive and reflect the light emitted by the light source.

[0016] In the above absorbance measurement device for repeatedly penetrating a sample to be measured, the number of times of N - time transmission of the optical fiber is greater than 2.

[0017] Compared with the existing technology, the advantages of the present utility model are as follows: In order to measure the absorbance of a solution to be measured with a low concentration and a small volume, the technical means adopted by the present invention is to repeatedly penetrate the liquid sample to be detected to increase the effective optical path passing through the liquid sample to be detected, so as to measure the corresponding absorbance value An, which is easily detected by the detection device. Under the condition that other conditions remain unchanged, the present invention can reduce the detection lower limit to 1 / N of the existing technology, greatly improving the detection range of the device. Description of the Drawings

[0018] Figure 1 It is a schematic diagram of the principle of Embodiment 1;

[0019] Figure 2 It is a schematic diagram of the principle of Embodiment 2;

[0020] Figure 3 It is a schematic diagram of the principle of Embodiment 3;

[0021] Figure 4 It is a schematic diagram of the principle of Embodiment 4;

[0022] Figure 5 It is a schematic diagram of the principle of Embodiment 5;

[0023] In the figure: light source 1, photoelectric sensor 2, optical fiber 3, mirror group 4, cuvette 5, enclosed space 6, first optical fiber head 7, second optical fiber head 71, first optical fiber head group 8, second optical fiber head group 81, liquid sample 9 to be detected. Detailed implementation manners

[0024] The following further describes the present utility model in detail with reference to the accompanying drawings and specific implementation manners.

[0025] An absorbance measurement device for repeatedly penetrating a sample to be measured includes a liquid sample 9 to be detected and a blank control sample. The liquid sample 9 to be detected and the blank control sample are loaded in a sample loading mechanism. A light source 1 for emitting penetrating light and a photoelectric sensor 2 for receiving light are respectively arranged outside the sample loading mechanism, and a plurality of optical fibers 3 or mirror groups 4 for receiving the light transmitted through the liquid sample 9 to be detected and reflecting it are arranged on both sides of the sample loading mechanism.

[0026] In this application, in one solution, an optical fiber 3 is used to receive and reflect the transmitted light. The first optical fiber collects the light Lt1 that first passes through the sample, and bends the first optical fiber 3 back to the sample interface to make Lt1 pass through the liquid sample 9 to be detected again to become Lt2. Then, the second optical fiber 3 collects the Lt2 that passes through the liquid sample 9 to be detected for the second time, and bends the second optical fiber 3 back to the interface of the liquid sample 9 to be detected to make Lt2 pass through the liquid sample 9 to be detected again to become Lt3, and so on. The light beam can penetrate the sample to be measured multiple times, and the number of times is N, and N is greater than 2 times, so as to increase the effective optical path of the light passing through the liquid sample 9 to be detected, and thus measure the corresponding absorbance value An. The absorbance A1 of a single penetration of the liquid sample 9 to be detected is A1 = An / N.

[0027] Another solution uses a mirror group for measurement. The first mirror group 4 reflects the light Lt1 that first passes through the liquid sample 9 to be detected, and allows Lt1 to pass through the liquid sample 9 to be detected again to become Lt2. The second mirror group 4 reflects Lt2 that second passes through the liquid sample 9 to be detected, and allows Lt2 to pass through the liquid sample 9 to be detected again to become Lt3, and so on. The light beam can penetrate the liquid sample 9 to be detected multiple times, with the number of times being N, and N is greater than 2 times, so as to increase the effective optical path passing through the liquid sample 9 to be detected, thereby measuring the corresponding absorbance value An. The absorbance A1 for a single penetration of the liquid sample 9 to be detected is A1 = An / N.

[0028] Embodiment 1

[0029] As Figure 1 shown, the sample loading mechanism is a cuvette 5, and the liquid sample 9 to be detected and the blank control sample are loaded in the cuvette 5.

[0030] Among them, one end of the cuvette 5 is provided with a light source 1 and one end is provided with a photoelectric sensor 2 for receiving the light that has passed through the optical fiber 3 for N times and passed through the liquid sample to be detected. The optical fiber 3 or the mirror group 4 is arranged at the two sides of the cuvette 5 in a staggered manner and receives and reflects the light emitted by the light source 1.

[0031] The cuvette 5 contains the liquid sample 9 to be detected or the control liquid sample. The light emitted by the light source 1 is transmitted through the optical fiber 3 and is detected by the photoelectric sensor 2 after passing through the liquid sample 9 to be detected N times. After obtaining the signals of the liquid sample 9 to be detected and the blank control sample, the absorbance value An for multiple penetrations of the sample is obtained, and the absorbance A1 for a single penetration of the sample is obtained through A1 = An / N.

[0032] Embodiment 2

[0033] As Figure 2 shown, the principle and structure of this embodiment are the same as those of Embodiment 1. The difference lies in that: the sample loading mechanism is an enclosed space 6, and the liquid sample 9 to be detected and the blank control sample are loaded in the enclosed space 6.

[0034] Among them, one end of the enclosed space 6 is provided with a light source 1 and one end is provided with a photoelectric sensor 2 for receiving the light that has passed through the optical fiber 3 for N times and passed through the liquid sample 9 to be detected. The optical fiber 3 or the mirror group 4 is arranged at the two sides of the enclosed space 6 in a staggered manner and receives and reflects the light emitted by the light source 1.

[0035] The enclosed space 6 contains the liquid sample 9 to be detected or a control liquid sample. The light emitted by the light source 2 is transmitted through the optical fiber 3, and after passing through the liquid sample 9 to be detected N times, it is detected by the photoelectric sensor 2. After obtaining the signals of the liquid sample 9 to be detected and the blank control sample, the absorbance value An of the liquid sample 9 to be detected after passing through multiple times is obtained, and the absorbance A1 of the liquid sample 9 to be detected for a single penetration is obtained through A1 = An / N. The opening and closing of the enclosed space 6 are controlled by an external device, which facilitates the user to take out and inject the liquid sample 9 to be detected or the control liquid sample, and clean the enclosed space 6.

[0036] Embodiment III

[0037] As Figure 3 shown, the principle and structure of this embodiment are the same as those of Embodiment I, and the difference lies in that: the sample loading mechanism is the first optical fiber head 7 and the second optical fiber head 71, and the liquid sample 9 to be detected and the blank control sample are arranged between the first optical fiber head 7 and the second optical fiber head 71.

[0038] Among them, a light source 1 is arranged at one end of the first optical fiber head 7 and the second optical fiber head 71, and a photoelectric sensor 2 for receiving the light that has passed through the optical fiber 3 N times and passed through the liquid sample 9 to be detected is arranged at one end. The optical fiber 3 or the mirror group 4 is arranged at the positions on both sides of the first optical fiber head 7 and the second optical fiber head 71 in a staggered manner to receive and reflect the light emitted by the light source 1.

[0039] The liquid sample 9 to be detected or the control liquid sample is contained between the optical fiber head 7 and the optical fiber head 71. The light emitted by the light source 1 is transmitted through the first optical fiber head 7 and the second optical fiber head 71, and after passing through the liquid sample 9 to be detected N times, it is detected by the photoelectric sensor 2. After the device obtains the signals of the liquid sample 9 to be detected and the blank control sample, the absorbance value An of the liquid sample 9 to be detected after passing through multiple times is obtained, and the absorbance A1 of the liquid sample 9 to be detected for a single penetration is obtained through A1 = An / N. The device can control the separation of the first optical fiber head 7 and the second optical fiber head 71, which facilitates the user to take out and inject the liquid sample 9 to be detected or the control liquid sample, and clean the optical fiber head 7 and the optical fiber head 71.

[0040] Embodiment IV

[0041] As Figure 4 shown, the principle and structure of this embodiment are the same as those of Embodiment I, and the difference lies in that: the sample loading mechanism is the first optical fiber head group 8 and the second optical fiber head group 81, and the liquid sample 9 to be detected and the blank control sample are arranged between the first optical fiber head group 8 and the second optical fiber head group 81.

[0042] Among them, a light source 1 is provided at one end of the first optical fiber head group 8 and the second optical fiber head group 81, and a photoelectric sensor 2 for receiving the light that has passed through the optical fiber 3 for N times and passed through the liquid sample 9 to be detected is provided at one end. The optical fiber 3 or the mirror group 4 is arranged at the positions on both sides of the first optical fiber head 7 and the second optical fiber head 71 in a staggered manner, and receives and reflects the light emitted by the light source 1.

[0043] A liquid sample 9 to be detected or a control liquid sample is placed between the first optical fiber head group 8 and the second optical fiber head group 81. The light emitted by the light source 1 is transmitted through the first optical fiber head group 8 and the second optical fiber head group 81, and after passing through the liquid sample 9 to be detected N times, it is detected by the photoelectric sensor 2. After the device obtains the signals of the liquid sample 9 to be detected and the blank control sample, it obtains the absorbance value An of the light passing through the liquid sample 9 to be detected multiple times, and calculates the absorbance A1 of the single penetration of the liquid sample 9 to be detected through A1 = An / N. The device can control the separation of the first optical fiber head group 8 and the second optical fiber head group 81 to facilitate the user to take out and inject the liquid sample 9 to be detected or the control liquid sample, and clean the first optical fiber head group 8 and the second optical fiber head group 81.

[0044] Embodiment 5

[0045] As Figure 5 shown, the principle and structure of this embodiment are the same as those of Embodiment 1. The difference lies in that: the sample loading mechanism is a closed space 6, the liquid sample 9 to be detected and the blank control sample are loaded in the closed space 6, and the first optical fiber head group 8 and the second optical fiber head group 81 are respectively provided on both sides of the liquid sample 9 to be detected in the closed space.

[0046] Among them, a light source 1 is provided at one end of the closed space 6, and a photoelectric sensor 2 for receiving the light that has passed through the optical fiber 3 for N times and passed through the liquid sample 9 to be detected is provided at the other end. The first optical fiber head group 8 and the second optical fiber head group 81 are arranged at the positions on both sides of the closed space 6 in a staggered manner, and receive and reflect the light emitted by the light source 1.

[0047] A liquid sample 9 to be detected or a control liquid sample is placed between the first optical fiber head group 8 and the second optical fiber head group 81. The light emitted by the light source 1 is transmitted through the optical fiber 3, and after passing through the liquid sample 9 to be detected N times, it is detected by the photoelectric sensor 2. After the device obtains the signals of the liquid sample 9 to be detected and the blank control sample, it obtains the absorbance value An of the light passing through the liquid sample 9 to be detected multiple times, and calculates the absorbance A1 of the single penetration of the liquid sample 9 to be detected through A1 = An / N. The device can control the separation of the first optical fiber head group 8 and the second optical fiber head group 81 to facilitate the user to take out and inject the liquid sample 9 to be detected, and clean the first optical fiber head group 8 and the second optical fiber head group 81.

[0048] Furthermore, the number of times of N - time transmission of the optical fiber is greater than 2.

[0049] In summary, the principle of this embodiment is as follows: in the present invention, the light beam penetrates the sample to be measured multiple times, the number of times is N, and N is greater than 2 times, so as to increase the effective optical path through the liquid sample 9 to be detected, and thus the corresponding absorbance value An is measured. The absorbance A1 of a single penetration through the liquid sample 9 to be detected is A1 = An / N. Compared with the prior art in which the light beam penetrates the liquid sample 9 to be detected once or twice, the effective optical path through the liquid sample 9 to be detected can be increased. Under the condition that other conditions remain unchanged, the measured absorbance value An is increased, and the absorbance A1 of a single penetration through the liquid sample 9 to be detected is obtained through A1 = An / N. When the concentration of the liquid sample 9 to be detected is relatively low, usually A1 is small and may be lower than the detection limit of the detection device, resulting in an inaccurate detection result. Compared with A1, the value of An is N times that of A1 and is larger than A1, making it easier to be detected by the detection device. Under the condition that other conditions remain unchanged, the present invention can reduce the detection limit to 1 / N of the prior art, greatly improving the detection range of the device.

[0050] The specific embodiments described herein are merely illustrative of the spirit of the present utility model. Those skilled in the art to which the present utility model pertains can make various modifications or supplements to the described specific embodiments or use similar ways to substitute them, but will not deviate from the spirit of the present utility model or exceed the scope defined by the appended claims.

Claims

1. An absorbance measuring device for multiple penetrations of a sample to be tested, comprising a liquid sample to be tested (9) and a blank control sample, characterized in that: The liquid sample to be detected (9) and the blank control sample are loaded in a sample loading mechanism. A light source (1) for emitting penetrating light and a photoelectric sensor (2) for receiving light are respectively arranged on the outside of the sample loading mechanism. A plurality of optical fibers (3) or a reflector group (4) for receiving and reflecting light transmitted through the liquid sample to be detected (9) are arranged on both sides of the sample loading mechanism.

2. The absorbance measuring device for multiple penetration of a sample to be tested according to claim 1, characterized in that: The sample loading mechanism is a cuvette (5), and the liquid sample (9) to be detected and the blank control sample are loaded in the cuvette (5).

3. The absorbance measuring device for multiple penetration of a sample to be measured according to claim 2, characterized in that: The cuvette (5) is provided with a light source (1) at one end thereof and a photoelectric sensor (2) at one end thereof for receiving light that has been transmitted N times through the optical fiber (3) and has passed through the liquid sample (9) to be detected. The optical fiber (3) or the reflector group (4) is staggeredly arranged at two sides of the cuvette (5) and receives and reflects the light emitted by the light source (1).

4. The absorbance measuring device for multiple penetration of a sample to be tested according to claim 1, characterized in that: The sample loading mechanism is a closed space (6), and the liquid sample to be tested (9) and the blank control sample are loaded in the closed space (6).

5. The absorbance measuring device for multiple penetration of a sample to be measured according to claim 4, characterized in that: A light source (1) is disposed at one end of the closed space (6), and a photoelectric sensor (2) is disposed at one end for receiving light that has been transmitted N times through the optical fiber (3) and has passed through the liquid sample (9) to be detected. The optical fiber (3) or the reflector group (4) is staggeredly disposed at two sides of the closed space (6) and receives and reflects the light emitted by the light source (1).

6. The absorbance measuring device for multiple penetration of a sample to be tested according to claim 1, characterized in that: The sample loading mechanism comprises a first optical fiber head (7) and a second optical fiber head (71), and the liquid sample to be detected (9) and the blank control sample are arranged between the first optical fiber head (7) and the second optical fiber head (71).

7. The absorbance measuring device for multiple penetration of a sample to be measured according to claim 6, characterized in that: One end of the first optical fiber head (7) and the second optical fiber head (71) is provided with a light source (1) and one end of the second optical fiber head (71) is provided with a photoelectric sensor (2) for receiving light transmitted N times through the optical fiber (3) and passing through the liquid sample (9) to be detected. The optical fiber (3) or the reflector group (4) is staggeredly arranged at two sides of the first optical fiber head (7) and the second optical fiber head (71) to receive and reflect the light emitted by the light source (1).

8. The absorbance measuring device for multiple penetrations of a sample to be tested according to claim 1, characterized in that: The sample loading mechanism comprises a first optical fiber head group (8) and a second optical fiber head group (81), and the liquid sample to be detected (9) and the blank control sample are arranged between the first optical fiber head group (8) and the second optical fiber head group (81).

9. The absorbance measuring device for multiple penetration of a sample to be measured according to claim 8, characterized in that: One end of the first optical fiber head group (8) and the second optical fiber head group (81) is provided with a light source (1) and one end of the second optical fiber head group (81) is provided with a photoelectric sensor (2) for receiving light transmitted N times through the optical fiber (3) and passing through the liquid sample (9) to be detected. The optical fiber (3) or the reflector group (4) is staggeredly arranged at two sides of the first optical fiber head group (8) and the second optical fiber head group (81) to receive and reflect the light emitted by the light source (1).

10. The absorbance measuring device for multiple penetration of a sample to be measured according to claim 9, characterized in that: The optical fiber (3) transmits N times more than 2 times.

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