Equipment for measuring content of uric acid in saliva by adopting electrochemical method

Abstract

The utility model discloses equipment for measuring the content of uric acid in saliva by adopting an electrochemical method, which belongs to the technical field of uric acid content detection equipment and comprises a saliva inlet hole, a movable sliding chute, a detection component, a test paper placing component and a mounting shell, and the movable sliding chute is formed in the left side wall of the mounting shell and extends into the mounting shell; the test paper placing assembly is in transmission connection in the movable sliding groove, the detection assembly is placed in the test paper placing assembly, the saliva inlet hole is formed in the upper surface of the mounting shell and extends downwards into the movable sliding groove, and the bottom of the saliva inlet hole makes contact with the rear end of the upper surface of the detection assembly. The problem that only the content of uric acid in blood can be detected due to the fact that an existing uric acid detection method is inaccurate in low content detection, low in content of uric acid in saliva and high in content of uric acid in blood in the prior art is solved. According to the equipment, the detection efficiency is improved while the detection process is simplified, the detection cost is reduced, and the universality of the equipment is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of uric acid content detection equipment, and particularly relates to a device for measuring the salivary uric acid content by an electrochemical method. Background Art

[0002] Saliva is a complex mixture of liquids, and its main components include proteins, urea, uric acid, microorganisms, enzymes, and various electrolytes. Like other body fluids such as blood and urine, the substances contained therein are extremely rich. Multiple studies have shown that the uric acid level in saliva has a strong correlation with the corresponding substance level in blood, and saliva can be used as a kind of "in vitro blood" for detection.

[0003] Uric acid is the final product obtained from the decomposition of purine nucleotides and is an important biomarker related to various diseases. It can be used as an important tool for various diseases, such as gout and hyperuricemia, or diseases related to increased oxidative stress. Utilize the uric acid concentration measured in saliva and study its association with various diseases. Salivary uric acid can be proven to be a non-invasive method for diagnosing serious diseases. Existing studies have shown that an increase in the uric acid level in saliva may be related to cancer, human immunodeficiency virus (HIV) infection, gout, and hypertension. On the other hand, a decrease in the salivary uric acid level may be a marker for the progression of Alzheimer's disease, multiple sclerosis, and cognitive impairment. A series of literature indicates that uric acid is an important biomarker. It is found that the assessment of salivary uric acid levels is crucial in diseases such as cancer, metabolic syndrome, neurological diseases, mental diseases, human immunodeficiency virus, and gout, as well as in monitoring the treatment of hyperuricemia.

[0004] The existing means for detecting uric acid mainly rely on serum determination. By extracting venous blood samples, using acetonitrile to precipitate the proteins in the serum, then filtering the proteins, and finally using a biochemical analyzer to measure the uric acid concentration in the serum. Not only does the extraction of blood cause physical and psychological stress to patients, but the detection method is relatively complex and the detection cost is relatively high. At the same time, if a portable uric acid meter is used to measure fingertip blood, it is necessary to prick the finger, and the user experience is also not good.

[0005] Therefore, how to provide a device for measuring the salivary uric acid content by an electrochemical method to solve the defects existing in the existing uric acid determination methods is an urgent technical problem to be solved by those skilled in the art. Summary of the Utility Model

[0006] For this reason, the utility model provides a device for measuring the salivary uric acid content by an electrochemical method to solve the problem that in the prior art, due to the inaccurate measurement of low content in the existing uric acid detection method, and the low uric acid content in saliva and high uric acid content in blood, it is only possible to detect the uric acid content in blood.

[0007] To achieve the above object, the utility model provides the following technical solutions:

[0008] The utility model discloses a device for measuring the salivary uric acid content by an electrochemical method, comprising:

[0009] An installation shell, a moving chute is opened on the left side wall, and the moving chute extends into the interior of the installation shell;

[0010] A test strip placing component, which is drivingly connected in the moving chute;

[0011] A detection component, which is placed in the test strip placing component;

[0012] A saliva inlet hole is opened on the upper surface of the installation shell;

[0013] Wherein, the saliva inlet hole extends downward into the moving chute, and the bottom of the saliva inlet hole is in contact with the rear end of the upper surface of the detection component.

[0014] In a possible implementation manner, the detection component includes:

[0015] A placing plate, which is installed on the upper surface of the test strip placing component, an electrochemical tester is arranged on the placing plate, and one end of the electrochemical tester is connected to the test strip placing component;

[0016] A three-electrode test strip, which is installed above the placing plate, and the three-electrode test strip is in contact with the other end of the electrochemical tester;

[0017] An interference removal layer, which is installed at the upper end of the three-electrode test strip;

[0018] An enzyme layer, which is installed above the interference removal layer, and the top end of the enzyme layer is in contact with the bottom end of the saliva inlet hole.

[0019] In a possible implementation manner, the interference removal layer adopts a carboxylated graphene film.

[0020] In a possible implementation manner, the enzyme layer is a cellulose acetate filter membrane loaded with uricase.

[0021] In a possible implementation manner, the test strip placing component includes:

[0022] A moving plate, which is drivingly connected in the moving chute;

[0023] An installation groove is opened on the upper surface of the moving plate, and the installation groove extends backward to the rear surface of the device moving plate;

[0024] A circuit sensor is arranged in the installation groove, one end of the circuit sensor is connected to the detection component, and the circuit sensor is signal-connected to a mobile phone.

[0025] In a possible implementation, the upper part of the saliva inlet hole is an inverted quadrangular frustum, and the lower part of the saliva inlet hole is a cuboid, and the top surface of the cuboid is connected to the small rectangular surface of the inverted quadrangular frustum.

[0026] In the present utility model, the detection component is placed in the test strip placement component, and then the test strip placement component is inserted into the installation housing through the moving chute. After installation, the installation housing is inserted into the oral cavity, and saliva flows down along the saliva inlet hole. The saliva flows into the detection component for signal conversion, converting the chemical signal in the saliva into an electrical signal and giving feedback. When detecting next time, only the detection component needs to be replaced to perform the detection again. This not only simplifies the detection operation, but also effectively reduces the detection cost by only replacing the detection component, improving the versatility of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the embodiments of the present utility model or the technical solutions in the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the drawings in the following description are only exemplary, and for those of ordinary skill in the art, other implementation drawings can be obtained according to the provided drawings without creative efforts.

[0028] The structures, ratios, sizes, etc. illustrated in this specification are only used to cooperate with the content disclosed in the specification for those who are familiar with this technology to understand and read, and are not used to limit the limiting conditions that the present utility model can be implemented. Therefore, they do not have technical substance significance. Any modification of the structure, change of the proportional relationship, or adjustment of the size, without affecting the effects that the present utility model can produce and the purposes that can be achieved, should still fall within the scope that the technical content disclosed by the present utility model can cover.

[0029] Figure 1 Is a three-dimensional view of the device for measuring the content of salivary uric acid by the electrochemical method provided by the present utility model;

[0030] Figure 2 Is a three-dimensional view of the detection component provided by the present utility model;

[0031] Figure 3 Is a three-dimensional view of the test strip placement component provided by the present utility model;

[0032] In the figure: 1 saliva inlet hole; 2 moving chute; 3 detection component; 31 enzyme layer; 32 interference removal layer; 33 placement plate; 34 three-electrode test strip; 4 test strip placement component; 41 moving plate; 42 installation groove; 5 installation housing. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0033] The following is a specific embodiment of the present invention. People familiar with the technology can easily understand the other advantages and functions of the present invention from the contents disclosed in this specification. Obviously, the described embodiment is a part of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0034] Please refer to Figures 1 - 3 Now, a device for measuring salivary uric acid content by electrochemical method disclosed in the utility model is described. The utility model is composed of five parts, such as Figure 1 , including a saliva inlet hole 1, a movable chute 2, a detection component 3, a test paper placement component 4 and an installation shell 5, the left wall of the installation shell 5 is provided with a movable chute 2, the movable chute 2 extends to the inside of the installation shell 5, the test paper placement component 4 is connected in a transmission manner in the movable chute 2, the detection component 3 is placed in the test paper placement component 4, the saliva inlet hole 1 is provided on the upper surface of the installation shell 5, the saliva inlet hole 1 extends downward into the movable chute 2, and the bottom of the saliva inlet hole 1 contacts the rear end of the upper surface of the detection component 3. When the utility model is used, the detection component 3 is prepared and installed in the test paper placement component 4, and then the rear of the upper surface of the detection component 3 is aligned with the saliva inlet hole 1 through the sliding design of the test paper placement component 4 and the movable chute 2, and then the installation shell 5 is extended into the mouth, the saliva will flow along the saliva inlet hole 1, and will flow all the way to the detection component 3, and the chemical signal will be converted into an electrical signal for feedback through the detection component 3, and finally the uric acid content detection in the saliva is completed, and when the detection is performed again, only the detection component 3 needs to be replaced, and then the above operation can be repeated to perform the detection again.

[0035] In a specific embodiment, Figure 2 , the detection component 3 includes an enzyme layer 31, an interference removal layer 32, a placement plate 33 and a three-electrode test paper 34, the placement plate 33 is installed on the upper surface of the test paper placement component 4, an electrochemical tester is arranged on the placement plate 33, one end of the electrochemical tester is connected to the test paper placement component 4, the three-electrode test paper 34 is installed above the placement plate 33, the three-electrode test paper 34 is in contact with the other end of the electrochemical tester, the interference removal layer 32 is installed on the upper end of the three-electrode test paper 34, the enzyme layer 31 is installed above the interference removal layer 32, and the top of the enzyme layer 31 is in contact with the bottom of the saliva inlet hole 1. The detection principle is that uric acid in saliva specifically binds to the enzyme layer 31 to generate H2O2, and H2O2 separates the substances that interfere with the signal in the saliva during the process of passing through the interference removal layer 32, and then H2O2 is decomposed by the three-electrode test paper, and the decomposition current is detected and recorded by the matching electrochemical tester, and the current size can reflect the content of uric acid.

[0036] On the basis of the previous embodiment, the interference removal layer 32 adopts a carboxylated graphene film. The carboxylated graphene film can resist endogenous or exogenous reducing interfering substances in body fluids such as ascorbic acid, dopamine, acetaminophen (the main ingredient of antipyretic drugs), and realize real-time accurate interference-free detection of uric acid. The preparation method of the carboxylated graphene film is to prepare a cGO-cMWCNT composite material, disperse 100mg GO in 100ml deionized water, and ultrasonically treat it for 30min. 6g NaOH and 5g ClCH2COOH are added to the above solution, and ultrasonicated for 3h at room temperature. The product is centrifuged and washed to obtain a precipitate 1. 20mg cMWCNT and 6ml deionized water are added to the precipitate 1, and ultrasonicated for 3h at room temperature to obtain a cGO-cMWCNT composite material, and then a 2mm diameter platinum disk electrode is polished to a mirror surface with alumina polishing powder (0.3μm), and then the electrode surface is rinsed clean with deionized water and anhydrous ethanol, and dried naturally. 10 μL of cGO-cMWCNT dispersion was dropped on the surface of the platinum electrode and dried at room temperature for 2 h to obtain cGO-cMWCNT / Pt. First, 1 μL of 4U·μL cGO-cMWCNT dispersion was coated on the surface of the Pt electrode. -1 urate oxidase (UOx), and then 10 μL of cGO-cMWCNT dispersion was added. After natural drying, the electrochemical uric acid sensor cGO-cMWCNT / UOx / Pt was obtained.

[0037] On the basis of the previous embodiment, the enzyme layer 31 is a cellulose acetate filter membrane loaded with urate oxidase. Uric acid and urate oxidase specifically bind to each other to generate H2O2. The cellulose acetate filter membrane has the advantages of small pore size, compact structure, fast filtration rate, low production cost, etc. It also has good thermal stability, wide pH range, and surface active hydroxyl groups.

[0038] In a specific embodiment, Figure 3 The test strip placement component 4 includes a moving plate 41 and a mounting groove 42. The moving plate 41 is connected to the moving slide groove 2 by transmission. The mounting groove 42 is provided on the upper surface of the moving plate 41. The mounting groove 42 extends backward to the rear surface of the device moving plate 41. The circuit sensor is arranged in the mounting groove 42. One end of the circuit sensor is connected to the detection component 3. The circuit sensor is connected to the mobile phone through a signal. The circuit sensor can be connected to the electrochemical tester on the uric acid test strip, and the data collected is transmitted to the mobile phone terminal for signal feedback.

[0039] In a specific embodiment, the upper part of the saliva inlet hole 1 is an inverted quadrangular frustum, and the lower part of the saliva inlet hole 1 is a cuboid. The top surface of the cuboid is connected to the small rectangular surface of the inverted quadrangular frustum. The inverted quadrangular frustum has its large rectangular surface arranged on the upper surface of the installation housing 5 to increase the saliva receiving area and accelerate the collection of saliva. The side surface of the inverted quadrangular frustum is inclined, which can effectively accelerate the saliva flow rate. Then, the inflow channel is arranged as a cuboid, and the design of its vertical surface can prevent saliva from hanging on the wall.

[0040] Although the present utility model has been described in detail above with general descriptions and specific embodiments, based on the present utility model, some modifications or improvements can be made, which are obvious to those skilled in the art. Therefore, these modifications or improvements made without departing from the spirit of the present utility model all fall within the scope of protection required by the present utility model.

Claims

1. A device for measuring salivary uric acid content using an electrochemical method, characterized in that: include: The installation shell (5) has a movable slide groove (2) on its left side wall, and the movable slide groove (2) extends into the interior of the installation shell (5); A test paper placement assembly (4) is transmission-connected in the movable slide groove (2); A detection component (3) is placed in the test paper placement component (4); A saliva inlet hole (1) is provided on the upper surface of the mounting housing (5); The saliva inlet hole (1) extends downward into the movable slide groove (2), and the bottom of the saliva inlet hole (1) contacts the rear end of the upper surface of the detection component (3).

2. The device for measuring salivary uric acid content by electrochemical method according to claim 1, characterized in that: The detection component (3) comprises: A placement plate (33) is mounted on the upper surface of the test paper placement assembly (4); an electrochemical tester is arranged on the placement plate (33); one end of the electrochemical tester is connected to the test paper placement assembly (4); A three-electrode test paper (34) is installed above the placement plate (33), and the three-electrode test paper (34) is in contact with the other end of the electrochemical tester; An interference removal layer (32) is mounted on the upper end of the three-electrode test paper (34); The enzyme layer (31) is installed above the interference removal layer (32), and the top of the enzyme layer (31) is in contact with the bottom of the saliva inlet hole (1).

3. The device for measuring salivary uric acid content by electrochemical method according to claim 2, characterized in that: The interferent removal layer (32) is a carboxylated graphene film.

4. The device for measuring salivary uric acid content by electrochemical method according to claim 2, characterized in that: The enzyme layer (31) is a cellulose acetate filter membrane loaded with urate oxidase.

5. The device for measuring salivary uric acid content by electrochemical method according to claim 1, characterized in that: The test paper placement component (4) comprises: A movable plate (41) drivingly connected in the movable slide groove (2); A mounting groove (42) is provided on the upper surface of the movable plate (41), and the mounting groove (42) extends backward to the rear surface of the movable plate (41); A circuit sensor is arranged in the installation groove (42), one end of the circuit sensor is connected to the detection component (3), and the circuit sensor is connected to the mobile phone via a signal.

6. The device for measuring salivary uric acid content by electrochemical method according to claim 1, characterized in that: The upper part of the saliva inlet hole (1) is an inverted quadrangular pyramid, and the lower part of the saliva inlet hole (1) is a rectangular parallelepiped, and the top surface of the rectangular parallelepiped is connected to the small rectangular surface of the inverted quadrangular pyramid.

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