Automatic reagent quantity control device for protein separation and purification

Abstract

The utility model discloses an automatic reagent quantity control device for protein separation and purification, which comprises a shell, a sealing cover is fixed on the outer wall of the front surface of the shell through a bolt, a material guide mechanism is arranged on one side of the bottom of the shell, the outer wall of the top of the shell is in threaded connection with feed ports which are distributed at equal intervals, and the feed ports are communicated with the sealing cover. The device comprises a shell, the outer wall of one side of the shell is in threaded connection with a cleaning opening, one side of the cleaning opening is in threaded connection with an inserting pipe, a uniformizing mechanism is arranged in the inserting pipe and comprises connecting pipes, impellers, balloons and isolating membranes, and the connecting pipes are connected to the circumference of the inserting pipe in a sleeving mode at equal intervals; and the connecting pipe and the inserting pipe are mutually communicated and sealed. According to the utility model, the balloon is arranged between the mounting box and the connecting pipe, so that self-adaptive expansion can be realized, a sealing effect is achieved, the resistance of a reagent can be adjusted, the flow and the speed of the reagent can be controlled, and the problems of abrasion and corrosion of the reagent to a pipeline are avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of reagent metering, in particular to an automatic reagent metering device for protein separation and purification. Background Art

[0002] The automatic metering of protein separation and purification reagents refers to the precise metering operation of protein separation and purification reagents by using automated equipment and technologies. This automatic metering method can greatly improve the accuracy and efficiency of experiments. Protein separation and purification reagents usually exist in the form of solids or liquids, and their usage needs to be precisely controlled according to experimental requirements. The traditional method is to perform metering by weighing or using a volumeter, but this method has certain errors and is cumbersome to operate. Using automated equipment and technologies for the automatic metering of protein separation and purification reagents can solve the above problems. A common method is to use an automated liquid handling system, which can automatically control the addition amount of reagents according to preset parameters. For example, the corresponding reagent amount can be automatically added by setting the required weight or volume.

[0003] In the existing protein separation and purification process, various reagents need to be added. When various reagents are put into the reaction process, they cannot be quickly and effectively evenly distributed, resulting in a reduction in the efficiency of protein separation and purification. At the same time, there are large errors, leading to a decline in the quality of protein separation and purification. Therefore, there is an urgent need for an automatic reagent metering device for protein separation and purification. Summary of the Utility Model

[0004] The purpose of the utility model is to solve the shortcomings existing in the prior art, and to propose an automatic reagent metering device for protein separation and purification.

[0005] In order to achieve the above purpose, the utility model adopts the following technical scheme:

[0006] An automatic reagent metering device for protein separation and purification, comprising a housing, the front outer wall of the housing is fixed with a sealing cover by bolts, and a feeding mechanism is arranged on one side of the bottom of the housing. The top outer wall of the housing is connected with a plurality of equidistantly distributed feeding ports by threads, and one side outer wall of the housing is connected with a cleaning port by threads. One side of the cleaning port is connected with an insertion pipe by threads, and a uniform mechanism is arranged inside the insertion pipe.

[0007] As a further scheme of the utility model: the uniform mechanism includes a connecting pipe, an impeller, a balloon and an isolation membrane, and the connecting pipes are sleeved on the circumference of the insertion pipe at equal distances. The connecting pipe is in sealed communication with the insertion pipe, and the bottom outer wall of the connecting pipe is connected with an installation box by threads.

[0008] As a further solution of the utility model: Each of the impellers is rotatably connected inside the installation box, and a balloon is arranged between the installation box and the connecting pipe. The isolation membrane is adhesively bonded to the inner wall at the communication position of the connecting pipe and the insertion pipe at equal intervals. The bottom of the feed inlet is connected to the connecting pipe through a thread.

[0009] As a further solution of the utility model: The outer wall of the bottom of the installation box is connected with a storage pipe through a thread, and a heating ring is arranged on the outer wall of the bottom of the storage pipe.

[0010] As a further solution of the utility model: The feeding mechanism includes a filter screen, a flow valve, a U-shaped converging pipe, an L-shaped feeding pipe and a sealing installation ring, and the L-shaped feeding pipe is connected to the front outer wall of the bottom of the storage pipe through a thread.

[0011] As a further solution of the utility model: The middle section of the U-shaped converging pipe is connected with an installation pipe, and the filter screens are respectively arranged on the inner wall of the end of the installation pipe and the inner walls of both ends of the U-shaped converging pipe.

[0012] As a further solution of the utility model: The flow valves are respectively fixedly connected to the end of the installation pipe and the end of the U-shaped converging pipe, and the L-shaped feeding pipe is connected to the outer wall on one side of the middle of the U-shaped converging pipe through a sealing installation ring.

[0013] Compared with the prior art, the utility model provides an automatic reagent metering device for protein separation and purification, which has the following beneficial effects:

[0014] For the automatic reagent metering device for protein separation and purification, by introducing reagents with different functions into the three feed inlets, the reagents enter the connecting pipe and then enter the installation box along the connecting pipe. The impellers inside drive the reagents to rotate, which not only effectively stirs the reagents to make the internal components evenly distributed, but also plays a certain speed-reducing role to ensure the controllability of subsequent reagent injection. At the same time, the balloon arranged between the installation box and the connecting pipe can achieve adaptive expansion, which not only plays a sealing role, but also can adjust the resistance of the reagent, control the flow rate and speed of the reagent, and also avoid the wear and corrosion problems of the reagent to the pipeline.

[0015] For the automatic reagent metering device for protein separation and purification, the reagent enters the U-shaped converging pipe and the installation pipe through the storage pipe. First, it is filtered by the filter screens at its ends to ensure the cleanliness of the internal components of the reagent. Subsequently, the reagent converges and enters the L-shaped feeding pipe, and is introduced into the reaction kettle for protein separation and purification by it. Among them, the flow valves located at the end of the installation pipe and the end of the U-shaped converging pipe can regulate the output of different reagents, ensuring the quality of the protein separation and purification reaction.

[0016] The parts not involved in this device are the same as those in the prior art or can be implemented using the prior art. The structure of this utility model is simple and the operation is convenient. Brief Description of the Drawings

[0017] Figure 1 It is a front view structural schematic diagram of an automatic reagent metering device for protein separation and purification proposed by this utility model;

[0018] Figure 2 It is an internal structural schematic diagram of an automatic reagent metering device for protein separation and purification proposed by this utility model;

[0019] Figure 3 It is a structural schematic diagram of a uniform mechanism in an automatic reagent metering device for protein separation and purification proposed by this utility model;

[0020] Figure 4 It is a structural schematic diagram of a material guiding mechanism in an automatic reagent metering device for protein separation and purification proposed by this utility model.

[0021] In the figure: 1. Housing; 2. Sealing cover; 3. Material guiding mechanism; 4. Cleaning port; 5. Feeding port; 6. Insertion connecting pipe; 7. Uniform mechanism; 8. Installation box; 9. Stock pipe; 10. Heating ring; 301. Filter screen; 302. Flow valve; 303. U-shaped converging pipe; 304. L-shaped material guiding pipe; 305. Sealing installation ring; 701. Connecting pipe; 702. Impeller; 703. Balloon; 704. Isolation membrane. Detailed Description of the Preferred Embodiments

[0022] Next, the technical solutions in the embodiments of this utility model will be clearly and completely described in conjunction with the drawings in the embodiments of this utility model. Obviously, the described embodiments are only a part of the embodiments of this utility model, rather than all of the embodiments.

[0023] In the description of this patent, it should be understood that the orientation or positional relationships indicated by the terms "center", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. are based on the orientation or positional relationships shown in the drawings, and are only for the convenience of describing this patent and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to this patent.

[0024] In the description of this patent, it should be noted that unless otherwise clearly specified and defined, the terms "installation", "connection", "linkage", and "setting" should be understood in a broad sense. For example, it can be fixedly connected and set, or detachably connected and set, or integrally connected and set. For those of ordinary skill in the art, the specific meanings of the above terms in this patent can be understood according to specific circumstances.

[0025] An automatic reagent metering device for protein separation and purification, as Figure 1 , Figure 2 , Figure 3 and Figure 4 shown, includes a housing 1. A sealing cover 2 is fixedly attached to the front outer wall of the housing 1 by bolts. A feeding mechanism 3 is provided on one side of the bottom of the housing 1. The top outer wall of the housing 1 is threadedly connected with equidistantly distributed feeding ports 5. A cleaning port 4 is threadedly connected to one side outer wall of the housing 1. A plug-in pipe 6 is threadedly connected to one side of the cleaning port 4. A homogenizing mechanism 7 is provided inside the plug-in pipe 6.

[0026] To ensure the uniformity of the reagent during protein separation and purification, as Figure 2 , Figure 3 and Figure 3 shown, the homogenizing mechanism 7 includes a connecting pipe 701, an impeller 702, a balloon 703, and a diaphragm 704. The connecting pipes 701 are equidistantly sleeved on the circumference of the plug-in pipe 6. The connecting pipe 701 is in sealed communication with the plug-in pipe 6. The bottom outer wall of the connecting pipe 701 is threadedly connected with a mounting box 8. Each impeller 702 is rotatably connected inside the mounting box 8. A balloon 703 is provided between the mounting box 8 and the connecting pipe 701. The balloon 703 is made of silicone rubber material. The diaphragms 704 are adhesively bonded to the inner wall at the communication part of the connecting pipe 701 and the plug-in pipe 6 at equal intervals. The bottom of the feeding port 5 is threadedly connected to the connecting pipe 701.

[0027] The bottom outer wall of the mounting box 8 is threadedly connected with a storage pipe 9. A heating ring 10 is provided on the bottom outer wall of the storage pipe 9. Through the setting of the heating ring 10, it effectively ensures that the reagent does not caking or precipitate in the storage pipe 9.

[0028] By introducing reagents with different functions into the three feeding ports 5, the reagents enter the connecting pipe 701 and then flow into the mounting box 8 along the connecting pipe 701. The impeller 702 inside drives the reagents to rotate, which not only effectively stirs the reagents to make their internal components evenly distributed, but also plays a certain speed-reducing role to ensure the controllability of subsequent reagent injection. At the same time, the balloon 703 provided between the mounting box 8 and the connecting pipe 701 can achieve self-adaptive expansion, which not only plays a sealing role, but also can adjust the resistance of the reagent, control the flow rate and speed of the reagent, and also avoid the wear and corrosion problems of the reagent to the pipeline.

[0029] To achieve controllability in adding protein separation and purification reagents, as Figure 4 shown, the material guiding mechanism 3 includes a filter screen 301, a flow valve 302, a U-shaped converging pipe 303, an L-shaped material guiding pipe 304, and a sealed mounting ring 305. The L-shaped material guiding pipe 304 is threadedly connected to the front outer wall of the bottom of the storage pipe 9. The middle section of the U-shaped converging pipe 303 is connected with a mounting pipe, and the filter screen 301 is respectively arranged on the inner walls of the ends of the mounting pipe and the two ends of the U-shaped converging pipe 303. The flow valves 302 are respectively fixedly connected to the ends of the mounting pipe and the ends of the U-shaped converging pipe 303. The L-shaped material guiding pipe 304 is connected to the outer wall on one side of the middle of the U-shaped converging pipe 303 through the sealed mounting ring 305. The model of the flow valve 302 is SRT-06;

[0030] The reagent enters the U-shaped converging pipe 303 and the mounting pipe through the storage pipe 9, and is first filtered by the filter screen 301 at its end to ensure the cleanliness of the internal components of the reagent. Subsequently, the reagent converges and enters the L-shaped material guiding pipe 304, and is introduced into the reaction kettle for protein separation and purification. Among them, the flow valves 302 located at the ends of the mounting pipe and the U-shaped converging pipe 303 can regulate the output of different reagents, ensuring the quality of the protein separation and purification reaction.

[0031] The above is only a preferred specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention, according to the technical solution and the inventive concept of the present invention, makes equivalent substitutions or changes, and should be covered within the protection scope of the present invention.

Claims

1. A reagent automatic quantity control device for protein separation and purification, comprising a housing (1), characterized in that: The front outer wall of the shell (1) is fixed with a sealing cover (2) by bolts, and a material guide mechanism (3) is provided on one side of the bottom of the shell (1), and the top outer wall of the shell (1) is connected with feed ports (5) distributed at equal distances by threads, and a cleaning port (4) is connected with one side of the outer wall of the shell (1) by threads, and a plug-in tube (6) is connected with one side of the cleaning port (4) by threads, and a uniform mechanism (7) is provided inside the plug-in tube (6).

2. The automatic amount control device for reagents for protein separation and purification according to claim 1, characterized in that: The uniform mechanism (7) comprises a connecting tube (701), an impeller (702), a balloon (703) and an isolation membrane (704), and the connecting tube (701) is sleeved on the circumference of the plug-in tube (6) at equal distances, the connecting tube (701) and the plug-in tube (6) are mutually connected and sealed, and the bottom outer wall of the connecting tube (701) is connected to a mounting box (8) via a thread.

3. The automatic reagent quantity control device for protein separation and purification according to claim 2, characterized in that: Each of the impellers (702) is rotatably connected to the installation box (8), and a balloon (703) is provided between the installation box (8) and the connecting pipe (701). The isolation membrane (704) is bonded to the inner wall of the connecting pipe (701) and the plug-in pipe (6) at equal distances, and the bottom of the feed port (5) is connected to the connecting pipe (701) via a thread.

4. The automatic reagent quantity control device for protein separation and purification according to claim 3, characterized in that: The bottom outer wall of the installation box (8) is connected to a material storage tube (9) via a thread, and the bottom outer wall of the material storage tube (9) is provided with a heating ring (10).

5. The automatic reagent quantity control device for protein separation and purification according to claim 1, characterized in that: The material guiding mechanism (3) comprises a filter screen (301), a flow valve (302), a U-shaped convergence pipe (303), an L-shaped material guiding pipe (304) and a sealing mounting ring (305), and the L-shaped material guiding pipe (304) is connected to the outer wall of the bottom front of the material storage pipe (9) via threads.

6. The automatic reagent quantity control device for protein separation and purification according to claim 5, characterized in that: The middle section of the U-shaped converging tube (303) is connected to a mounting tube, and the filter screen (301) is respectively arranged on the inner wall of the end of the mounting tube and the inner walls of both ends of the U-shaped converging tube (303).

7. The automatic reagent quantity control device for protein separation and purification according to claim 5, characterized in that: The flow valve (302) is fixedly connected to the end of the mounting pipe and the end of the U-shaped converging pipe (303), respectively, and the L-shaped material guide pipe (304) is connected to the outer wall of one side in the middle of the U-shaped converging pipe (303) via a sealing mounting ring (305).

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