Liquid output mechanism

Abstract

The utility model belongs to the field of molecular detection, and particularly relates to a liquid output mechanism. When the liquid output mechanism is used, a storage tube and a reaction tube are correspondingly fixed to a sample position and a detection position on a carrier respectively, then a driving part is pressed to slide on the carrier, and in the sliding process of the driving part, the driving part firstly drives an adapter in a first puncture assembly to move; enabling the first needle head positioned on the first needle head to penetrate into the reaction tube; then the sliding part is pushed, so that the puncture needle on the sliding part is punctured into the storage tube, the communication between the storage tube and the reaction tube is realized, the sample liquid in the storage tube can enter the reaction tube through the infusion tube, that is, the liquid in the storage tube can be transferred to the reaction tube by pressing the driving part, the operation is simple and convenient, and errors are not easy to occur; and the risk of biological exposure pollution does not exist.

Description

Technical Field

[0001] The present application relates to the field of molecular detection technologies, and particularly to a liquid output mechanism. Background Art

[0002] POCT, point-of-care testing, refers to clinical testing and bedside testing conducted beside patients, usually not necessarily by clinical laboratory technicians. It is a new method of analyzing immediately at the sampling site, eliminating the complex processing procedures of specimens during laboratory testing and obtaining test results quickly. The key to realizing molecular POCT is to simplify sample processing. Direct amplification / Direct PCR is a technology that can achieve in vitro nucleic acid amplification without additional nucleic acid extraction, eliminating the cumbersome procedure of extracting genomic DNA and can be directly applied to rapid PCR detection.

[0003] The existing molecular detection experimental process using the direct amplification method is as follows: after collecting pharyngeal or nasal swabs from patients, they are placed in a cell preservation solution tube for storage. Then, manually or by a machine, the lid is opened to aspirate a part of the sample liquid and add it into a PCR tube filled with dispensed PCR reagents, and then it is put on a machine for amplification detection. The number of consumables used in the whole process is relatively large, the liquid transfer requires manual operation, there are also many operation steps, it is easy to make mistakes, and there is also a risk of biological exposure and contamination. Summary of the Utility Model

[0004] The embodiments of the present application provide a liquid output mechanism, aiming to simplify the operation process of transferring sample liquid between the preservation tube and the PCR tube and reduce the experimental cost.

[0005] To this end, according to one aspect of the present application, a liquid output mechanism is provided for outputting the liquid in a preservation tube to a reaction tube. The liquid output mechanism includes:

[0006] A carrier having a sample position for fixing the preservation tube and a detection position for fixing the reaction tube;

[0007] A driving member slidably disposed on the carrier;

[0008] A first puncture assembly including an adapter movably connected to the driving member and a first needle disposed on the adapter, and the reaction tube is located on the movement path of the two first needles;

[0009] A second puncture assembly including a sliding member slidably disposed on the carrier and a puncture needle disposed on the sliding member, and the preservation tube is located on the movement path of the sliding member;

[0010] An infusion tube, one end of which is connected to the puncture needle and the other end is connected to the first needle;

[0011] Among them, the movement path of the driving member intersects the movement path of the sliding member. There are at least a starting position, a pre-position, and an intermediate position on the movement path of the driving member. During the process of the driving member moving from the starting position to the pre-position, the driving member drives the first needle on the adapter to pierce into the reaction tube; during the process of the driving member moving from the pre-position to the intermediate position, the driving member pushes the sliding member, so that the puncture needle pierces into the storage tube.

[0012] Optionally, a pushing inclined surface is provided on the sliding member, and a pushing inclined surface is provided on the driving member. During the process of the driving member moving from the starting position to the pre-position, the pushing inclined surface gradually approaches and abuts against the pushing inclined surface. During the process of the driving member moving from the pre-position to the intermediate position, the pushing inclined surface pushes the pushing inclined surface, so that the sliding member drives the first needle to pierce into the storage tube.

[0013] Optionally, the sliding member is slidably arranged on the carrier along a first direction, and the driving member is slidably arranged on the carrier along a second direction, and the second direction is perpendicular to the first direction.

[0014] Optionally, it further includes a suction assembly and a second needle arranged on the adapter. On the movement path of the driving member, there is also a termination position after the intermediate position; the suction assembly includes a suction member and a suction pipe with one end communicated with the suction port of the suction member, and the other end of the suction pipe is connected to the second needle;

[0015] During the process of the driving member moving from the intermediate position to the termination position, the driving member triggers the suction member to quantitatively extract the gas in the reaction tube, so that the liquid in the storage tube is quantitatively inhaled into the reaction tube through the infusion tube.

[0016] Optionally, when the driving member is in the pre-position, the adapter is disengaged from the driving member. During the process of the driving member moving from the pre-position to the termination position, the adapter is stationary relative to the carrier.

[0017] Optionally, the driving member has two side plates arranged at intervals, and the adapter has two side strips arranged at intervals, the relative outer sides of the two side strips are clamped with the relative inner sides of the two side plates, and a separation structure is provided on the carrier on the movement path of the two side plates. In the process of the driving member moving from the starting position to the front position, the adapter moves with the driving member to drive the first needle and the second needle to penetrate the reaction tube; in the process of the driving member moving from the front position to the middle position, the separation structure is inserted into the relative inner sides of the two side plates and spreads the two side plates apart to separate the side strips from the side plates.

[0018] Optionally, the suction member includes a piston cylinder and a piston slidably disposed in the piston cylinder, the end of the suction pipe away from the reaction tube is connected to the interior of the piston cylinder, and the piston is connected to the driving member; when the driving member moves to the middle position, the piston cylinder is limited on the carrier, and in the process of the driving member moving from the middle position to the end position, the piston and the piston cylinder move relative to each other, thereby quantitatively extracting the gas in the reaction tube through the suction pipe.

[0019] Optionally, the carrier is a hollow shell structure with a accommodating cavity inside, and the sample position, the detection position, the first puncture assembly, the second puncture assembly and the infusion tube are all arranged in the accommodating cavity. The top of the carrier is provided with a first insertion port for the preservation tube to be inserted into the sample position, and the bottom of the carrier is provided with a second insertion port for the reaction tube to be inserted into the detection position.

[0020] Optionally, the liquid output mechanism further includes a push rod, one end of which is connected to the driving member, and the other end of which extends out of the accommodating cavity.

[0021] Optionally, a receiving groove is further provided on the carrier, one end of the push rod is hinged to the driving member, and the push rod can be flipped relative to the driving member and received in the receiving groove.

[0022] The beneficial effects of the liquid output mechanism provided by this application are as follows: Compared with the prior art, when the liquid output mechanism of this application is actually used, the storage tube and the reaction tube are respectively fixed on the sample position and the detection position of the carrier, and then the driving member is pressed to slide on the carrier. During the sliding process of the driving member, the driving member first drives the adapter in the first puncture assembly to move, so that the first needle on it pierces into the reaction tube; then it pushes the sliding member, so that the puncture needle on the sliding member pierces into the storage tube, realizing the connection between the storage tube and the reaction tube through the infusion tube, enabling the sample liquid in the storage tube to enter the reaction tube through the infusion tube. That is, by pressing the driving member, the sample liquid in the storage tube can be transferred to the reaction tube. The operation is simple and convenient, not easy to make mistakes, and there is no risk of biological exposure and contamination, improving the accuracy of the molecular direct amplification detection result. Description of the Drawings

[0023] In order to more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the following drawings are only some embodiments of this application. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on these drawings.

[0024] Among them:

[0025] Figure 1 is the external structural schematic diagram of the molecular direct amplification detection device shown in an embodiment of this application;

[0026] Figure 2 is the structural schematic diagram of the liquid output mechanism shown in an embodiment of this application after removing the carrier and with the driving member in the starting position;

[0027] Figure 3 is the cross-sectional structural schematic diagram of the carrier of the liquid output mechanism shown in an embodiment of this application;

[0028] Figure 4 is the cross-sectional structural schematic diagram of the liquid output mechanism shown in an embodiment of this application with the driving member in the pre-position;

[0029] Figure 5 is the cross-sectional structural schematic diagram of the liquid output mechanism shown in an embodiment of this application with the driving member in the middle position;

[0030] Figure 6 is the cross-sectional structural schematic diagram of the liquid output mechanism shown in an embodiment of this application with the driving member in the termination position;

[0031] Figure 7 is the structural schematic diagram of the liquid output mechanism shown in an embodiment of this application when the driving member and the adapter are not separated;

[0032] Figure 8 It is a schematic structural diagram after the separation between the driving member and the adapter in the liquid output mechanism shown in an embodiment of the present application.

[0033] Description of main component symbols:

[0034] 10. Storage tube; 11. Puncture part; 12. Tube cap; 13. Support column;

[0035] 20. Reaction tube; 21. Rubber stopper;

[0036] 100. Carrier; 1001. First insertion port; 1002. Second insertion port; 1003. Storage groove; 101. Slide groove; 102. Guide groove; 103. Guide slot; 110. Separation structure;

[0037] 200. First puncture assembly; 210. Adapter; 211. Side strip; 2111. Clamping protrusion; 212. Connection block; 220. First needle; 230. Second needle;

[0038] 300. Infusion tube;

[0039] 400. Driving member; 401. Abutting inclined surface; 410. Guide block; 420. Side plate; 421. Card slot;

[0040] 500. Push rod;

[0041] 600. Suction member; 610. Piston cylinder; 611. Guide rib; 620. Piston;

[0042] 700. Suction tube;

[0043] 800. Piston rod;

[0044] 900. Second puncture assembly; 910. Sliding member; 911. Pushing inclined surface; 920. Puncture needle. Detailed implementation manners

[0045] To facilitate the understanding of the present application, the present application will be described more comprehensively below with reference to the relevant drawings. Preferred embodiments of the present application are shown in the drawings. However, the present application can be implemented in many other different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the understanding of the disclosure content of the present application more thorough and comprehensive.

[0046] It should be noted that when an element is referred to as "fixed to" or "disposed on" another element, it can be directly on the other element or indirectly on the other element. When an element is referred to as "connected to" another element, it can be directly connected to the other element or indirectly connected to the other element.

[0047] It should be understood that the orientation or positional relationship indicated by terms such as "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present application.

[0048] In addition, the terms "first" and "second" are only used for descriptive purposes and should not be construed as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include one or more of such features. In the description of the present application, the meaning of "a plurality" is two or more, unless otherwise specifically defined.

[0049] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which this application belongs. The terms used in the specification of this application are only for the purpose of describing specific embodiments and are not intended to limit this application.

[0050] It should also be noted that in the embodiments of the present application, the same reference numerals are used to represent the same components or the same parts. For the same parts in the embodiments of the present application, only one of the parts or components may be marked with a reference numeral in the drawings. It should be understood that the reference numerals are equally applicable to other identical parts or components.

[0051] Embodiments of the present application provide a liquid output mechanism for outputting the liquid in the storage tube to the reaction tube, as Figures 2 - 5 shown, the liquid output mechanism includes a carrier 100, a driving member 400, a first puncture assembly 200, a second puncture assembly 900, an infusion tube 300, and a suction assembly.

[0052] The carrier 100 has a sample position for fixing the storage tube 10 and a detection position for fixing the reaction tube 20. The driving member 400 is slidably disposed on the carrier 100. The first puncture assembly 200 includes an adapter 210 movably connected to the driving member 400 and a first needle 220 disposed on the adapter 210, and the reaction tube 20 is located on the movement path of the first needle 220. The second puncture assembly 900 includes a slider 910 slidably disposed on the carrier 100 and a puncture needle 920 disposed on the slider 910, and the storage tube 10 is located on the movement path of the slider 910. One end of the infusion tube 300 is connected to the puncture needle 920, and the other end is connected to the first needle 220.

[0053] Among them, the movement path of the driving member 400 intersects with the movement path of the sliding member 910, and there is at least a starting position on the movement path of the driving member 400 ( Figure 2 the position shown in Figure 4 ), a pre-position ( Figure 5 the position shown in

[0054] ), and an intermediate position (

[0055] the position shown in Figure 2 ). During the process of the driving member 400 moving from the starting position to the pre-position, the driving member 400 drives the first needle 220 on the adapter 210 to pierce into the reaction tube 20; during the process of the driving member 400 moving from the pre-position to the intermediate position, the driving member 400 pushes the sliding member 910, so that the puncture needle 920 pierces into the storage tube 10.

[0056] In the embodiment of the present application, when the liquid output mechanism is actually used, the storage tube 10 and the reaction tube 20 are respectively fixed at the sample position and the detection position on the carrier 100, and then the driving member 400 is pressed to make the driving member 400 slide on the carrier 100. During the sliding process of the driving member 400, the driving member 400 first drives the adapter 210 in the first puncturing assembly 200 to move, so that the first needle 220 located thereon pierces into the reaction tube 20. The driving member 400 continues to move and pushes the sliding member 910, so that the puncture needle 920 on the sliding member 910 pierces into the storage tube 10, realizing the connection between the storage tube 10 and the reaction tube 20 through the infusion tube 300, enabling the sample liquid in the storage tube 10 to enter the reaction tube 20 through the infusion tube 300. The operation is simple and convenient, not easy to make mistakes, and there is no risk of biological exposure and contamination, improving the accuracy of the detection results of the molecular direct amplification detection device. Figure 2 and Figures 4 - 6 shown, a pushing inclined surface 911 is provided on the sliding member 910, and a pushing inclined surface 401 is provided on the driving member 400. During the movement of the driving member 400 on the carrier 100, the pushing inclined surface 401 pushes the pushing inclined surface 911, so that the sliding member 910 drives the puncture needle 920 to pierce into the storage tube 10. Specifically, during the process of the driving member 400 moving from the starting position to the pre-position, the pushing inclined surface 401 gradually approaches and abuts against the pushing inclined surface 911. During the process of the driving member 400 moving from the pre-position to the intermediate position, the pushing inclined surface 401 pushes the pushing inclined surface 911, so that the sliding member 910 drives the first needle 220 to pierce into the storage tube 10.

[0057] It can be understood that the abutting inclined surface 401 can also be provided only on the driving member 400, or the pushing inclined surface 911 can be provided only on the sliding member 910. In this embodiment, the pushing inclined surface 911 on the sliding member 910 is pushed by the abutting inclined surface 401 on the carrier 100, increasing the contact area and improving the stability of the sliding member 910 during movement.

[0058] In one embodiment, as Figure 2 and Figures 4 - 6 shown, the sliding member 910 is slidably arranged on the carrier 100 in the first direction, and the driving member 400 is slidably arranged on the carrier 100 in the second direction, and the second direction is perpendicular to the first direction.

[0059] Specifically, the first direction is the horizontal direction in the figure, and the second direction is the vertical direction in the figure, which is also the height direction of the storage tube 10 and the reaction tube 20. With this setting, it is convenient to apply force to the driving member 400, and when in use, only need to press down the driving member 400.

[0060] Combined with Figures 2 - 4 shown, the carrier 100 is provided with a guiding groove 102 extending in the first direction, the sliding member 910 is slidably arranged in the guiding groove 102, one end of the guiding groove 102 is located on the movement path of the driving member 400, and the other end of the guiding groove 102 extends to the sample position. The carrier 100 is provided with a guiding groove 103 extending in the second direction, and the driving member 400 is provided with a guiding block 410, and the guiding block 410 is slidably arranged in the guiding groove 103. When not in use, the sliding member 910 is at one end of the guiding groove 102 located on the movement path of the driving member 400. At this time, the puncture needle 920 on the sliding member 910 does not penetrate into the storage tube 10. When pressing the driving member 400 to make the driving member 400 slide relative to the carrier 100, as Figures 4 - 5 shown, the driving member 400 can push the sliding member 910, driving the sliding member 910 to move along the guiding groove 102 towards the sample position and finally penetrate into the storage tube 10.

[0061] Specifically, the driving member 400 is provided with a driving rod, and the abutting inclined surface 401 is arranged on the driving rod. The size of the sliding member 910 (such as a slider) in the first direction is the same as the distance from the side of the driving rod close to the storage tube 10 to the storage tube 10 in this direction. With this setting, it can not only ensure that the sliding member 910 drives the puncture needle 920 to penetrate into the storage tube 10 under the pushing of the driving rod, but also make the sliding member 910 located on one side of the driving rod after the puncture needle 920 penetrates into the storage tube 10, without hindering the continuous downward movement of the driving member 400.

[0062] In one embodiment, as Figures 2 - 6 shown, it further includes a suction assembly and a second needle 230 arranged on the adapter 210. On the movement path of the driving member 400, there is also a termination position after the middle position ( Figure 6the position shown in ; the aspiration assembly includes an aspirator 600 and an aspiration tube 700 with one end communicating with the aspiration port of the aspirator 600, and the other end of the aspiration tube 700 is connected to the second needle 230; during the process of the driving member 400 moving from the intermediate position to the end position, the driving member 400 triggers the aspirator 600 to quantitatively extract the gas in the reaction tube 20, so that the liquid in the storage tube 10 is quantitatively inhaled into the reaction tube 20 through the infusion tube 300. During the process of the driving member 400 moving from the intermediate position to the end position, the side surface of the driving member 400 is in sliding contact with the side surface of the sliding member 910.

[0063] In a specific embodiment, as Figures 4 - 6 shown, when the driving member 400 is in the pre-position, the adapter 210 is disengaged from the driving member 400. During the process of the driving member 400 moving from the pre-position to the end position, the adapter 210 is stationary relative to the carrier 100, that is, stationary relative to the reaction tube 20.

[0064] It can be understood that since the reaction tube 20 is fixed at the reaction position on the carrier 100, after the first needle 220 and the second needle 230 on the adapter 210 penetrate into the reaction tube 20, if the adapter 210 and the driving member 400 are not disengaged, the adapter 210 in contact with the reaction tube 20 will hinder the driving member 400 from continuing to move from the pre-position to the end position.

[0065] In a specific embodiment, as Figures 7 - 8 shown, the driving member 400 has two spaced side plates 420, and the adapter 210 has two spaced side strips 211. The relative outer sides of the two side strips 211 are clamped with the relative inner sides of the two side plates 420. A separation structure 110 is provided on the carrier 100 on the movement path of the two side plates 420. During the process of the driving member 400 moving from the starting position to the pre-position, the adapter 210 moves together with the driving member 400 to drive the first needle 220 and the second needle 230 to penetrate into the reaction tube 20; during the process of the driving member 400 moving from the pre-position to the intermediate position, the separation structure 110 is inserted into the relative inner sides of the two side plates 420 and spreads the two side plates 420 apart, so that the side strips 211 are separated from the side plates 420.

[0066] Specifically, the relative inner sides of the two side plates 420 are provided with card slots 421. One ends of the two side strips 211 are connected by a connecting block 212. The first needle 220 and the second needle 230 are arranged on the connecting block 212. The relative outer sides of the other ends of the two side strips 211 are provided with card protrusions 2111. The adapter 210 is clamped in the card slots 421 on the relative inner sides of the two side plates 420 through the card protrusions 2111 at the ends of the two side strips 211.

[0067] In one implementation, the separation structure 110 is two separated pieces. When in the pre-position, the two separated pieces are inserted into the opposite inner sides of the two side plates 420 to expand the two side plates 420, so that the clamping protrusions 2111 at the ends of the side strips 211 are separated from the clamping grooves 421 in the side plates 420, the adapter 210 is disengaged from the driving member 400, and remains relatively stationary with respect to the reaction tube 20 during the subsequent movement of the driving member 400.

[0068] In another specific embodiment, not shown in the figure, a sleeve is provided on the driving member, the axial direction of the sleeve is along the movement direction of the driving member on the carrier, a plug rod with an outer diameter smaller than the inner diameter of the sleeve is provided on the adapter, the first needle and the second needle are arranged at one end of the plug rod, the other end of the plug rod is inserted into the sleeve, a spring positioning bead is provided on the outer wall of one end of the plug rod inserted into the sleeve, and a hemispherical groove adapted to the positioning bead is provided on the inner wall of the sleeve. The spring positioning bead is snapped into the hemispherical groove to realize the connection between the adapter and the driving member. During the process of the driving member moving from the starting position to the pre-position, the adapter moves together with the driving member to drive the two needles to pierce into the reaction tube. As the driving member is continuously pressed, the spring positioning bead snapped into the hemispherical groove disengages from the hemispherical groove, so that during the subsequent movement of the driving member, the driving member no longer applies force to the adapter.

[0069] In one embodiment, as Figures 2 - 6 shown, the suction member 600 includes a piston cylinder 610 and a piston 620 slidably disposed within the piston cylinder 610. One end of the suction tube 700 remote from the reaction tube 20 communicates with the interior of the piston cylinder 610, and the piston 620 is connected to the driving member 400 (specifically, a piston rod 800 is provided on the driving member 400, and the piston 620 is disposed on the piston rod 800); when the driving member 400 moves to the intermediate position, the piston cylinder 610 is limited on the carrier 100, so that during the process of the driving member 400 moving from the intermediate position to the end position, the piston 620 and the piston cylinder 610 move relative to each other, and then quantitatively extract the gas in the reaction tube 20 through the suction tube 700.

[0070] The gas in the reaction tube 20 is extracted by the movement of the piston 620 within the piston cylinder 610. The structure is simple, and the amount of gas extracted is determined by the inner diameter of the piston cylinder 610 and the distance that the piston 620 moves relative to the piston cylinder 610 during the process of the driving member 400 moving from the intermediate position to the end position, which is easy to control.

[0071] The carrier 100 is provided with a sliding groove 101 extending along the moving direction of the driving member 400. The length of the sliding groove 101 is the same as the moving distance of the driving member 400 from the starting position to the middle position. A guiding rib 611 is provided on the outer wall of the piston cylinder 610, and the guiding rib 611 is slidably arranged in the sliding groove 101. During the process of the driving member 400 moving from the starting position to the middle position, the guiding rib 611 slides in the sliding groove 101, and the cooperation between the two plays a role in guiding the movement of the piston cylinder 610; at the middle position, the guiding rib 611 abuts against the lower groove wall of the sliding groove 101, and the groove wall here of the sliding groove 101 forms a limiting portion to limit the piston cylinder 610 from continuing to move downward with the driving member 400, so as to realize that the piston cylinder 610 is stationary relative to the carrier 100 during the process of the driving member 400 moving from the middle position to the ending position.

[0072] In some embodiments, such as Figure 1 and Figures 4 - 6 shown, the carrier 100 is a hollow shell-like structure with an accommodating cavity inside. The sample position, the detection position, the first puncturing assembly 200, the second puncturing assembly 900, the infusion tube 300, and the suction assembly are all arranged in the accommodating cavity. A first insertion opening 1001 for inserting the storage tube 10 into the sample position is provided at the top of the carrier 100, and a second insertion opening 1002 for inserting the reaction tube 20 into the detection position is provided at the bottom of the carrier 100.

[0073] The carrier 100 is set as a hollow shell-like structure to protect the structures located inside it by the carrier 100, and also to prevent the puncturing needle 920 in the first puncturing assembly 200 and the first needle 220 and the second needle 230 in the second puncturing assembly 900 from leaking out and being contaminated or stabbing the user.

[0074] To facilitate the operation of the driving member 400, the liquid output mechanism further includes a push rod 500. One end of the push rod 500 is connected to the driving member 400, and the other end extends out of the accommodating cavity.

[0075] Preferably, a storage groove 1003 is further provided on the carrier 100. One end of the push rod 500 is hinged to the driving member 400, and the push rod 500 can be flipped relative to the driving member 400 and stored in the storage groove 1003. By providing the storage groove 1003, the push rod 500 can be stored before use, and the push rod 500 can also be prevented from being accidentally touched to cause the driving member 400 to be pushed.

[0076] The embodiment of the present application further provides a molecular direct amplification detection device, such as Figures 1 - 6As shown in the figure, the molecular direct amplification detection device includes the liquid output mechanism, the storage tube 10, and the reaction tube 20 in any of the above embodiments. The storage tube 10 is fixed at the sample position. A storage solution is stored in the storage tube 10. The lower end of the storage tube 10 has a puncture portion 11 that can be pierced by the puncture needle 920. The puncture portion 11 is located on the movement path of the puncture needle 920 in the second puncture assembly 900. The reaction tube 20 is fixed at the detection position. A detection reagent is stored in the reaction tube 20. The mouth of the reaction tube 20 is sealed by a rubber stopper 21 that can be pierced by a needle. The rubber stopper 21 is located on the movement path of the first needle 220 in the first puncture assembly 200.

[0077] Specifically, a through hole is provided at the lower end of the storage tube 10, and the through hole is sealed by a rubber cover, and the rubber cover forms the puncture portion 11 that can be pierced by the puncture needle 920.

[0078] Preferably, a support column 13 is provided at the bottom of the storage tube 10 to support the sampling swab broken into the storage tube 10, so as to prevent the puncture needle 920 from piercing into the sampling swab and blocking the puncture needle 920.

[0079] In summary, in one example, the storage tube 10 is a sampling tube, a cell storage solution is stored in the sampling tube, the reaction tube 20 is a PCR tube, paraffin balls and a PCR reagent freeze are stored in the PCR tube, and the entire usage process of the molecular direct amplification detection device is as follows:

[0080] 1. Open the tube cap 12 of the sampling tube, break the sampling swab into the tube, and tighten the tube cap 12;

[0081] 2. As shown in the figure, turn up the push rod 500; Figure 1

[0082] 3. As shown in and, press the push rod 500, and the driving member 400 drives the first needle 220 and the second needle 230 on the adapter 210 above the PCR tube to pierce the rubber stopper 21 of the PCR tube; Figure 2 Figure 4 and

[0083] 4. As shown in the figure, continue to press the push rod 500, the adapter 210 above the PCR tube is separated from the driving member 400, and the driving member 400 drives the puncture needle 920 on the slider on the side of the sample liquid tube to pierce the puncture portion 11 at the lower end of the sampling tube; Figure 5

[0084] 5. As shown in the figure, continue to press the push rod 500. After the piston cylinder 610 reaches the limit position, the piston 620 moves downward under the drive of the driving member 400 to start pumping the gas in the PCR tube, and quantitatively extracts the liquid containing the sample in the sampling tube into the PCR tube to re-dissolve the reagent freeze-dried balls. Figure 6

[0085] ​​​​The technical features of the above embodiments can be combined arbitrarily. For the sake of concise description, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, it should be considered as the scope recorded in this specification.

[0086] The above embodiments only express several implementation manners of the present application, and their descriptions are relatively specific and detailed, but they should not be construed as limiting the scope of the application. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present application, several modifications and improvements can still be made, and these all belong to the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the appended claims.

Claims

1. A liquid output mechanism for outputting the liquid in a storage tube (10) to a reaction tube (20), characterized in that: The liquid output mechanism comprises: A carrier (100) having a sample position for fixing the storage tube (10) and a detection position for fixing the reaction tube (20); A driving member (400) is slidably disposed on the carrier (100); A first puncture assembly (200) comprises a transfer member (210) movably connected to the driving member (400) and a first needle (220) disposed on the transfer member (210), wherein the reaction tube (20) is located on a movement path of the first needle (220); A second puncture assembly (900) comprises a sliding member (910) slidably disposed on the carrier (100) and a puncture needle (920) disposed on the sliding member (910), wherein the storage tube (10) is located on a movement path of the sliding member (910); An infusion tube (300), one end of which is connected to the puncture needle (920), and the other end of which is connected to the first needle (220); The movement path of the driving member (400) intersects with the movement path of the sliding member (910), and the movement path of the driving member (400) has at least a starting position, a front position and an intermediate position. When the driving member (400) moves from the starting position to the front position, the driving member (400) drives the first needle (220) on the adapter (210) to pierce the reaction tube (20); when the driving member (400) moves from the front position to the intermediate position, the driving member (400) pushes the sliding member (910) to enable the puncture needle (920) to pierce the storage tube (10).

2. The liquid output mechanism according to claim 1, characterized in that: The sliding member (910) is provided with a resisting inclined surface (911), and the driving member (400) is provided with a resisting inclined surface (401). When the driving member (400) moves from the starting position to the front position, the resisting inclined surface (401) gradually approaches and resists against the resisting inclined surface (911). When the driving member (400) moves from the front position to the middle position, the resisting inclined surface (401) pushes against the resisting inclined surface (911), so that the sliding member (910) drives the first needle (220) to penetrate into the storage tube (10).

3. The liquid output mechanism according to claim 1, characterized in that: The sliding member (910) is slidably disposed on the carrier (100) along a first direction, and the driving member (400) is slidably disposed on the carrier (100) along a second direction, wherein the second direction is perpendicular to the first direction.

4. The liquid output mechanism according to claim 1, characterized in that: It also includes a suction assembly and a second needle (230) arranged on the adapter, and has a termination position after the intermediate position on the movement path of the driving member (400); the suction assembly includes a suction member (600) and an air suction pipe (700) whose one end is connected to the suction port of the suction member (600), and the other end of the air suction pipe (700) is connected to the second needle (230); During the process of the driving member (400) moving from the intermediate position to the end position, the driving member (400) triggers the suction member (600) to quantitatively extract the gas in the reaction tube (20), so that the liquid in the storage tube (10) is quantitatively sucked into the reaction tube (20) through the infusion tube (300).

5. The liquid output mechanism according to claim 4, characterized in that: When the driving member (400) is in the front position, the adapter (210) is disengaged from the driving member (400), and when the driving member (400) moves from the front position to the end position, the adapter (210) is stationary relative to the carrier (100).

6. The liquid output mechanism according to claim 5, characterized in that: The driving member (400) has two spaced-apart side panels (420), the adapter (210) has two spaced-apart side strips (211), the relative outer sides of the two side strips (211) are engaged with the relative inner sides of the two side panels (420), and a separation structure (110) is provided on the carrier (100) on the movement path of the two side panels (420). When the driving member (400) moves from the starting position to the front position, the The adapter (210) moves together with the driving member (400) to drive the first needle (220) and the second needle (230) to penetrate the reaction tube (20); when the driving member (400) moves from the front position to the middle position, the separation structure (110) is inserted into the relative inner sides of the two side plates (420) and spreads the two side plates (420) apart to separate the side strip (211) from the side plates (420).

7. The liquid output mechanism according to claim 4 or 5, characterized in that: The suction member (600) includes a piston cylinder (610) and a piston (620) slidably disposed in the piston cylinder (610); one end of the suction pipe (700) away from the reaction tube (20) is connected to the interior of the piston cylinder (610), and the piston (620) is connected to the driving member (400); when the driving member (400) moves to the intermediate position, the piston cylinder (610) is limited on the carrier (100); in the process of the driving member (400) moving from the intermediate position to the end position, the piston (620) and the piston cylinder (610) move relative to each other, thereby quantitatively extracting the gas in the reaction tube (20) through the suction pipe (700).

8. The liquid output mechanism according to claim 1, characterized in that: The carrier (100) is a hollow shell-like structure having an internal accommodating cavity, wherein the sample position, the detection position, the first puncture assembly (200), the second puncture assembly (900) and the infusion tube (300) are all arranged in the accommodating cavity, and the top of the carrier (100) is provided with a first insertion port (1001) for inserting the preservation tube (10) into the sample position, and the bottom of the carrier (100) is provided with a second insertion port (1002) for inserting the reaction tube (20) into the detection position.

9. The liquid output mechanism according to claim 8, characterized in that: The liquid output mechanism further comprises a push rod (500), one end of which is connected to the driving member (400) and the other end of which extends out of the accommodating cavity.

10. The liquid output mechanism according to claim 9, characterized in that: The carrier (100) is also provided with a storage groove (1003), one end of the push rod (500) is hinged to the driving member (400), and the push rod (500) can be turned over relative to the driving member (400) and stored in the storage groove (1003).

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