Tumor tissue cell mechanical dissociation device

Abstract

The utility model relates to a tissue dissociation device, in particular to a mechanical dissociation device for tumor tissue cells, which comprises a tissue dissociation device body and is characterized in that limiting components are arranged on two sides of the tissue dissociation device body, and collision pieces are arranged on the limiting components; according to the tissue dissociation device, the limiting assembly, the abutting piece, the linkage piece, the top frame and the clamping piece are arranged on the tissue dissociation device body, the multiple tissue dissociation pipes can be taken down in batches when the multiple tissue dissociation pipes on the tissue dissociation device body are taken down, and the tissue dissociation pipes can be taken down by adjusting a second bolt and a third bolt. The first clamping plate and the second clamping plate are staggered, so that the first clamping plate and the second clamping plate can clamp the tissue dissociation tubes on the tissue dissociation device body, the linkage piece is lifted as a whole by kneading the linkage piece, the top frame is lifted, the clamped multiple tissue dissociation tubes are driven, and the tissue dissociation tubes on the tissue dissociation device body are separated from the tissue dissociation device body. And the tissue dissociator is taken down from the tissue dissociator body in batches, so that the operation convenience is improved.

Description

Technical Field

[0001] The utility model relates to a tissue dissociation device, in particular to a tumor tissue cell mechanical dissociation device. Background Art

[0002] The mechanical dissociation device for tumor tissue cells, commonly known as a "tissue dissociator" or "mechanical dissociator", is a device specially designed to separate single cells or small cell clusters from tumor tissue samples. It uses mechanical force to destroy the tissue structure, promote the separation between cells, and maintain the biological activity of cells as much as possible. This device has important application value in biomedical research, clinical medicine and drug development.

[0003] When the current mechanical dissociation device for tumor tissue cells is in use, the tissue dissociation tube is generally installed on the tissue dissociator by plugging and engaging. After the tissue inside the tissue dissociation tube is dissociated, the operator generally pulls out the tissue dissociation tube plugged in the tissue dissociator and removes it. When the tissue dissociation tube is pulled out, the operator needs to press the tissue dissociator with one hand and grasp the tissue dissociation tube with the other hand to remove it. The tissue dissociator generally has multiple tissue dissociation tubes installed to perform dissociation operations at the same time. When facing a large number of tissue dissociation tubes to be removed, the tissue dissociation tubes cannot be removed in batches, which increases the operator's operation workload and cannot achieve the effect of conveniently removing the tissue dissociation tubes. Utility Model Content

[0004] The purpose of the utility model is to provide a tumor tissue cell mechanical dissociation device to solve the problems raised in the above background technology.

[0005] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0006] A mechanical dissociation device for tumor tissue cells comprises: a tissue dissociator body, both sides of which are provided with limit assemblies, the limit assemblies are provided with a resistance piece, the two limit assemblies are rotatably connected to the bottom of a linkage piece, the tops of the two linkage pieces are respectively rotatably connected to the two ends of a top frame, and the top frame is provided with a clamping piece.

[0007] The mechanical dissociation device for tumor tissue cells as described above: the limiting assembly includes a limiting block, a guide column and an embedding groove, both ends of the limiting block are connected to the guide column, a rotation groove is provided on the limiting block on one side of the guide column, an embedding groove is provided on the side of the limiting block close to the side wall of the tissue dissociator body, and a threaded hole is provided on the limiting block at the embedding groove.

[0008] The tumor tissue cell mechanical dissociation device as described above: the resistance member includes a resistance plate and a first bolt rotatably arranged on one side of the resistance plate, and a silicone anti-skid pad is arranged on the side of the resistance plate away from the first bolt.

[0009] The mechanical dissociation device for tumor tissue cells as described above: the abutment plate is slidably disposed in the embedding groove, and the first bolt is threadedly connected in a threaded hole provided on the limiting block.

[0010] The mechanical dissociation device for tumor tissue cells as described above: the linkage part includes a first rotating rod, a second rotating rod, a pushing rod and a telescopic plug rod, the first rotating rod and the second rotating rod are rotatably connected via a rotating shaft, the first rotating rod and the second rotating rod are symmetrically arranged in two groups, the pushing rod is connected to the rotating shaft, and the two pushing rods are connected via a telescopic plug rod.

[0011] The mechanical dissociation device for tumor tissue cells as described above: guide holes are provided at the four corners of the top frame, a plurality of circular slots are provided at equal intervals on the top frame, rotation slots are provided at the bottoms of both ends of the top frame, threaded holes are provided on the side surfaces of both ends of the top frame, and the lateral surface of the top frame is arranged as a hollow cavity.

[0012] The tumor tissue cell mechanical dissociation device as described above: the other end of the second rotating rod is rotatably connected to the rotating groove opened on the limit block, and the other end of the first rotating rod is rotatably connected to the rotating groove opened on the top frame.

[0013] The mechanical dissociation device for tumor tissue cells as described above: the clamping member includes a first clamping plate, a second bolt, a plug-in slot and a second clamping plate, the first clamping plate is arranged in a C shape, one end of the solid part of the first clamping plate is rotatably connected to the second bolt, the hollow cavity of the first clamping plate forms a plug-in slot, the second clamping plate is slidably plugged in the plug-in slot, and the end of the second clamping plate facing away from the second bolt is rotatably connected to the third bolt.

[0014] As described above, the mechanical dissociation device for tumor tissue cells: the first clamping plate and the second clamping plate are slidably connected in the hollow cavity provided in the top frame, the second bolt is threadedly connected in the threaded hole at one end of the top frame, and the third bolt is threadedly connected in the threaded hole at the other end of the top frame, and the first clamping plate and the second clamping plate are both provided with a plurality of circular slots that are the same as the circular slots on the top frame.

[0015] Compared with the prior art, the beneficial effects of the utility model are:

[0016] By arranging a limit assembly, a resistance member, a linkage member, a top frame and a clamping member on the tissue dissociator body, it is possible to realize a batch removal operation of multiple tissue dissociation tubes on the tissue dissociator body when multiple tissue dissociation tubes on the tissue dissociator body are removed, and by adjusting the second bolt and the third bolt, the first clamping plate and the second clamping plate are misaligned, so that the first clamping plate and the second clamping plate can clamp the tissue dissociation tube on the tissue dissociator body, and by kneading the linkage member, the linkage member is raised as a whole, so that the top frame is raised, driving the clamped multiple tissue dissociation tubes to be removed from the tissue dissociator body in batches, thereby improving the convenience of operation. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 Schematic diagram of the structure of the mechanical dissociation device for tumor tissue cells.

[0018] Figure 2 Schematic diagram of the decomposed structure in the tumor tissue cell mechanical dissociation device.

[0019] Figure 3 This is a schematic diagram of the structure of the limiting component in the mechanical dissociation device for tumor tissue cells.

[0020] Figure 4 This is a schematic diagram of the structure of the resistance member in the mechanical dissociation device for tumor tissue cells.

[0021] Figure 5 This is a schematic diagram of the structure of the linkage parts in the mechanical dissociation device for tumor tissue cells.

[0022] Figure 6 This is a schematic diagram of the structure of the top frame in the tumor tissue cell mechanical dissociation device.

[0023] Figure 7 This is a schematic diagram of the structure of the clamping parts in the mechanical dissociation device for tumor tissue cells.

[0024] In the figure: 1. tissue dissociator body; 2. limit assembly; 201. limit block; 202. guide column; 203. embedding groove; 3. abutment member; 301. abutment plate; 302. first bolt; 4. linkage member; 401. first rotating rod; 402. second rotating rod; 403. push rod; 404. telescopic plug rod; 5. top frame; 501. guide hole; 6. clamping member; 601. first clamping plate; 602. second bolt; 603. plug-in groove; 604. second clamping plate; 605. third bolt. DETAILED DESCRIPTION

[0025] Various exemplary embodiments, features and aspects of the present application will be described in detail below with reference to the accompanying drawings. The same reference numerals in the accompanying drawings represent elements with the same or similar functions. Although various aspects of the embodiments are shown in the accompanying drawings, the drawings are not necessarily drawn to scale unless otherwise specified.

[0026] The word “exemplary” is used exclusively herein to mean “serving as an example, example, or illustration.” Any embodiment described herein as “exemplary” is not necessarily to be construed as preferred or advantageous over other embodiments.

[0027] In addition, in order to better illustrate the present application, numerous specific details are provided in the specific embodiments below. It should be understood by those skilled in the art that the present application can also be implemented without certain specific details. In some examples, methods, means, and elements well known to those skilled in the art are not described in detail in order to highlight the subject matter of the present application.

[0028] See also Figures 1 to 7 In an embodiment of the utility model, a mechanical dissociation device for tumor tissue cells includes: a tissue dissociator body 1, both sides of the tissue dissociator body 1 are provided with limit components 2, the limit components 2 are provided with a resistance member 3, the two limit components 2 are rotatably connected to the bottom of the linkage member 4, the tops of the two linkage members 4 are rotatably connected to the two ends of the top frame 5 respectively, and the top frame 5 is provided with a clamping member 6.

[0029] In this embodiment, when the tissue dissociation tube on the tissue dissociator body 1 is installed and used, the tissue dissociation tube is plugged into the insertion groove on the tissue dissociator body 1. At this time, the top frame 5 is in a state of contacting the top of the tissue dissociator body 1. The circular slots formed on the first clamping plate 601 and the second clamping plate 604 coincide with the circular slots formed on the top frame 5. The tissue dissociation tube is inserted into the circular slots, which does not hinder the plugging and engagement of the tissue dissociation tube on the tissue dissociator body 1, and the accessories for reinforcing the tissue dissociation tube are not hindered. After being installed and used, when it is necessary to remove multiple tissue dissociation tubes on the tissue dissociator body 1 at the same time, the operator uses both hands to synchronously rotate the second bolt 602 and the third bolt 605, and the first clamping plate 601 and the third bolt 605 are respectively threadedly connected to the threaded holes set at both ends of the top frame 5, and the first clamping plate 601 and the second clamping plate 604 are slidably connected in the hollow cavity of the top frame 5. Therefore, when the second bolt 602 and the third bolt 605 are rotated, the first clamping plate 601 and the second clamping plate 604 can be slidably adjusted.

[0030] When turning the second bolt 602, the first clamping plate 601 moves towards the side of the second bolt 602. When turning the third bolt 605, the second clamping plate 604 moves towards the side of the third bolt 605, so that the circular slot holes formed on the first clamping plate 601 and the second clamping plate 604 are misaligned. At this time, the combined space of the circular slot holes becomes smaller. Since the tissue dissociation tube is inserted into the tissue dissociator body 1 from the circular slot holes, when the space between the two circular slot holes becomes smaller, the tissue dissociation tube can be clamped in the reduced circular slot holes. Then, the operator synchronously pinches and adjusts the two linkage members 4 arranged on both sides of the tissue dissociator body 1 with both hands. By pinching the push rods 403 symmetrically arranged on the same side, the first rotating rod 401 and the second rotating rod 402 can be rotated. Since the rotation intersection points of the two groups of the first rotating rod 401 and the second rotating rod 402 are reduced in distance under the opposite pinching movement of the push rods 403, the combined height of the first rotating rod 401 and the second rotating rod 402 is increased.

[0031] By arranging two telescopic plug-in rods 404 between the two push rods 403, it can be ensured that the push rods 403 remain vertical during pinching, and there will be no problem of outward inclination affecting the synchronous movement of the two groups of the first rotating rod 401 and the second rotating rod 402, meeting the adjustment requirements. When the combined height of the first rotating rod 401 and the second rotating rod 402 is increased, since the linkage member 4 acts between the limit component 2 and the top frame 5, and the limit component 2 abuts and is fixed on both sides of the tissue dissociator body 1, the distance between the limit component 2 and the top frame 5 will increase at this time, so that the top frame 5 rises. Since the guiding holes 501 formed on the top frame 5 are slidably sleeved on the guiding columns 202, the top frame 5 can be made to rise vertically. The clamping member 6 clamping the tissue dissociation tube is arranged on the top frame 5. Therefore, when the top frame 5 rises, multiple tissue dissociation tubes on the tissue dissociator body 1 can be lifted and removed in batches, reducing the cumbersome operation of the operator taking out multiple tissue dissociation tubes individually one by one, and improving the use effect.

[0032] As a further solution of the present utility model, the limit component 2 includes a limit block 201, a guiding column 202 and an embedding groove 203. Both ends of the limit block 201 are connected with the guiding column 202. A rotating groove is formed on the limit block 201 on one side of the guiding column 202. An embedding groove 203 is formed on the limit block 201 close to the side wall of the tissue dissociator body 1, and a threaded hole is arranged on the limit block 201 at the position of the embedding groove 203.

[0033] In this embodiment, the spacing between the two limit assemblies 2 is greater than the lateral width of the tissue dissociator body 1. When the limit assemblies 2 are installed on the tissue dissociator body 1, the two ends of the limit block 201 need to be kept in a horizontal state to ensure that the top frame 5 can be vertically lifted and adjusted in the later stage to meet the operation of batch removal of multiple tissue dissociation tubes. The embedding groove 203 is used to make the contact plate 301 retract into the embedding groove 203 when the two limit assemblies 2 are attached to the two sides of the side wall of the tissue dissociator body 1. The contact plate 301 will not protrude, affecting the installation and use of the limit block 201 on the side wall of the tissue dissociator body 1.

[0034] As a further solution of the utility model, the resistance member 3 includes a resistance plate 301 and a first bolt 302 rotatably arranged on one side of the resistance plate 301, a silicone anti-slip pad is arranged on the side of the resistance plate 301 away from the first bolt 302, the resistance plate 301 is slidably arranged in the embedding groove 203, and the first bolt 302 is threadedly connected in a threaded hole opened on the limit block 201.

[0035] In this embodiment, the contact plate 301 can be telescopically slidably adjusted in the embedding groove 203 by rotating the first bolt 302. Since the length of the top frame 5 is fixed, the guide hole 501 is slidably sleeved on the guide column 202. Therefore, when the first bolt 302 is rotated to make the contact plate 301 contact the side wall of the tissue dissociator body 1, a thrust away from the side wall of the tissue dissociator body 1 is applied to the limit block 201, so that when the contact plate 301 is tightly contacted with the side wall of the tissue dissociator body 1, the position of the limit component 2 on the tissue dissociator body 1 can be positioned. The silicone anti-slip pad provided on the contact plate 301 can increase the friction between the contact plate 301 and the side wall of the tissue dissociator body 1, ensuring that the position of the limit component 2 is fixed and stable, and there will be no sliding problem on the side wall of the tissue dissociator body 1.

[0036] As a further solution of the utility model, the linkage 4 includes a first rotating rod 401, a second rotating rod 402, a pushing rod 403 and a telescopic plug rod 404. The first rotating rod 401 and the second rotating rod 402 are rotatably connected via a rotating shaft. The first rotating rod 401 and the second rotating rod 402 are symmetrically arranged in two groups. The pushing rod 403 is connected to the rotating shaft. The two pushing rods 403 are connected by a telescopic plug rod 404. The other end of the second rotating rod 402 is rotatably connected to the rotating groove opened on the limit block 201, and the other end of the first rotating rod 401 is rotatably connected to the rotating groove opened on the top frame 5.

[0037] In this embodiment, a telescopic plug-in rod 404 that can be telescopically plugged in is provided between the two push rods 403, so that when the push rods 403 are kneaded, the two push rods 403 can be kept vertical. Since the top of the first rotating rod 401 is rotatably connected to the rotating groove set at the bottom of the top frame 5, and the bottom of the second rotating rod 402 is rotatably connected to the rotating groove set on the limit block 201, when the two push rods 403 are kneaded, the intersection distance between the first rotating rod 401 and the second rotating rod 402 is reduced. At this time, the combined height of the first rotating rod 401 and the second rotating rod 402 will increase, realizing the operational use requirement of raising the top frame 5. When the pushing rods 403 are released, the top frame 5 falls back due to gravity, and the top frame 5 contacts the top cover of the tissue dissociator body 1, thereby resetting the linkage 4 as a whole.

[0038] As a further solution of the utility model, guide holes 501 are opened at the four corners of the top frame 5, a plurality of circular slots are opened at equal intervals on the top frame 5, rotation grooves are opened at the bottoms of both ends of the top frame 5, threaded holes are opened at the sides of both ends of the top frame 5, and the transverse surface of the top frame 5 is arranged in a hollow cavity.

[0039] In this embodiment, the guide hole 501 is slidably sleeved on the guide column 202, and the linkage member 4 acts between the limit assembly 2 and the top frame 5, so that when the top frame 5 is raised, it can ensure that the top frame 5 maintains horizontal lifting adjustment, meeting the needs of batch removal operations of multiple tissue dissociation tubes. The circular slots on the top frame 5 meet the needs of the plug-in and engagement of components such as tissue dissociation tubes on the tissue dissociator body 1, ensuring the use effect.

[0040] As a further solution of the utility model, the clamping member 6 includes a first clamping plate 601, a second bolt 602, a plug-in groove 603 and a second clamping plate 604. The first clamping plate 601 is arranged in a C shape, and one end of the solid part of the first clamping plate 601 is rotatably connected with the second bolt 602. The hollow cavity of the first clamping plate 601 forms a plug-in groove 603, and the second clamping plate 604 is slidably plugged in the plug-in groove 603. The end of the second clamping plate 604 away from the second bolt 602 is rotatably connected with the third bolt 605. The first clamping plate 601 and the second clamping plate 604 are slidably connected in the hollow cavity set in the top frame 5, the second bolt 602 is threadedly connected to the threaded hole at one end of the top frame 5, and the third bolt 605 is threadedly connected to the threaded hole at the other end of the top frame 5. The first clamping plate 601 and the second clamping plate 604 are both provided with a number of circular slots that are the same as the circular slots on the top frame 5.

[0041] In this embodiment, when adjusting the second bolt 602 and the third bolt 605, the second clamping plate 604 can slide and adjust within the insertion slot 603, and the first clamping plate 601 can slide and adjust within the hollow top frame 5. Since the circular slot holes formed on the first clamping plate 601 and the second clamping plate 604 are initially in a coincident state, when adjusting the movement of the first clamping plate 601 and the second clamping plate 604, the circular slot holes formed on the two will be misaligned, so that tissue dissociation tubes or components of different thicknesses can be clamped. In order to improve the clamping firmness of the circular slot holes on the tissue dissociation tube, anti-slip bumps or anti-slip pads can be provided on the inner walls of the circular slot holes formed on the first clamping plate 601 and the second clamping plate 604, so as to improve the firmness when clamping the tissue dissociation tube. After clamping the tissue dissociation tube, the clamping member 6 rises with the top frame 5, so as to realize the batch removal operation of multiple tissue dissociation tubes on the tissue dissociator body 1. After removing the tissue dissociation tube from the tissue dissociator body 1, by reversely adjusting the second bolt 602 and the third bolt 605, the circular slot holes on the first clamping plate 601 and the second clamping plate 604 are made to coincide, and the tissue dissociation tube can be conveniently taken out from the tissue dissociator body 1.

[0042] For those skilled in the art, it is obvious that the present utility model is not limited to the details of the above-described exemplary embodiments, and without departing from the spirit or basic characteristics of the present utility model, the present utility model can be implemented in other specific forms. Therefore, in any regard, the embodiments should be regarded as exemplary and non-restrictive. The scope of the present utility model is defined by the appended claims rather than the above description. Therefore, all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be included within the present utility model. Any reference signs in the claims should not be construed as limiting the claims involved.

[0043] In addition, it should be understood that although this specification is described in terms of embodiments, not every embodiment only contains an independent technical solution. This narrative manner of the specification is only for clarity. Those skilled in the art should regard the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A mechanical dissociation device for tumor tissue cells, comprising: The tissue dissociator body (1) is characterized in that both sides of the tissue dissociator body (1) are provided with limit assemblies (2), the limit assemblies (2) are provided with a resistance member (3), the two limit assemblies (2) are rotatably connected to the bottom of the linkage member (4), the tops of the two linkage members (4) are rotatably connected to the two ends of the top frame (5), and the top frame (5) is provided with a clamping member (6).

2. A tumor tissue cell mechanical dissociation device according to claim 1, characterized in that: The limiting assembly (2) comprises a limiting block (201), a guide post (202) and an embedding groove (203); both ends of the limiting block (201) are connected to the guide post (202); a rotation groove is provided on the limiting block (201) on one side of the guide post (202); an embedding groove (203) is provided on a side of the limiting block (201) close to the side wall of the tissue dissociator body (1); and a threaded hole is provided on the limiting block (201) at the embedding groove (203).

3. The mechanical dissociation device for tumor tissue cells according to claim 1, characterized in that: The resisting member (3) comprises a resisting plate (301) and a first bolt (302) rotatably arranged on one side of the resisting plate (301); a silicone anti-slip pad is arranged on one side of the resisting plate (301) away from the first bolt (302).

4. The mechanical dissociation device for tumor tissue cells according to claim 3, characterized in that: The abutment plate (301) is slidably disposed in the embedding groove (203), and the first bolt (302) is threadedly connected in a threaded hole provided on the limiting block (201).

5. The mechanical dissociation device for tumor tissue cells according to claim 1, characterized in that: The linkage member (4) comprises a first rotating rod (401), a second rotating rod (402), a pushing rod (403) and a telescopic plug rod (404); the first rotating rod (401) and the second rotating rod (402) are rotatably connected via a rotating shaft; two groups of the first rotating rod (401) and the second rotating rod (402) are symmetrically arranged; the pushing rod (403) is connected to the rotating shaft; and the two pushing rods (403) are connected via a telescopic plug rod (404).

6. The mechanical dissociation device for tumor tissue cells according to claim 1, characterized in that: The top frame (5) is provided with guide holes (501) at the four corners, the top frame (5) is provided with a plurality of circular slots at equal intervals, the bottoms of both ends of the top frame (5) are provided with rotation slots, the side surfaces of both ends of the top frame (5) are provided with threaded holes, and the transverse surface of the top frame (5) is provided with a hollow cavity.

7. The mechanical dissociation device for tumor tissue cells according to claim 5, characterized in that: The other end of the second rotating rod (402) is rotatably connected to a rotating groove provided on the limiting block (201), and the other end of the first rotating rod (401) is rotatably connected to a rotating groove provided on the top frame (5).

8. The mechanical dissociation device for tumor tissue cells according to claim 1, characterized in that: The clamping member (6) comprises a first clamping plate (601), a second bolt (602), a plug-in slot (603) and a second clamping plate (604); the first clamping plate (601) is arranged in a C shape; one end of a solid portion of the first clamping plate (601) is rotatably connected to the second bolt (602); a plug-in slot (603) is formed in a hollow cavity of the first clamping plate (601); the second clamping plate (604) is slidably plugged into the plug-in slot (603); and one end of the second clamping plate (604) facing away from the second bolt (602) is rotatably connected to the third bolt (605).

9. The mechanical dissociation device for tumor tissue cells according to claim 8, characterized in that: The first clamping plate (601) and the second clamping plate (604) are slidably connected in a hollow cavity provided in the top frame (5); the second bolt (602) is threadedly connected in a threaded hole at one end of the top frame (5); the third bolt (605) is threadedly connected in a threaded hole at the other end of the top frame (5); and the first clamping plate (601) and the second clamping plate (604) are both provided with a plurality of circular slots identical to the circular slots on the top frame (5).

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