Slide FISH (fluorescence in situ hybridization) pretreatment instrument

Abstract

The utility model relates to the technical field of medical instruments, and discloses a slide FISH (fluorescence in situ hybridization) pretreatment instrument which comprises a first turntable and a second turntable, a plurality of quantitative liquid adding components are uniformly distributed in the circumferential direction of the first turntable, and each quantitative liquid adding component comprises a reagent bin, a liquid adding valve and a liquid adding dropper which are communicated in sequence; a plurality of reaction units with reaction tanks are uniformly distributed in the circumferential direction of the second rotating disc, the reaction tanks are obliquely arranged, liquid inlets are formed in the tops of the reaction tanks, and liquid outlets are formed in the bottoms of the reaction tanks; any one liquid adding dropper can be aligned to the liquid inlet through rotation of the first rotating disc, and any one liquid inlet can be aligned to the liquid adding dropper through rotation of the second rotating disc. The problem that in the prior art, due to the fact that personnel operation levels are different, standardized operation is lacked, and errors of follow-up FISH detection are large is solved.

Description

Technical Field

[0001] The utility model relates to the technical field of medical devices, in particular to a slide FISH pre-processing instrument. Background Art

[0002] Fluorescence in situ hybridization (FISH) is a new technology that combines molecular biology and cytogenetics developed in the 1980s. Its principle is to use fluorescently labeled nucleic acid probes to hybridize with complementary regions of nucleic acids in samples. It has been widely used in many fields such as genetic disease diagnosis, viral infection analysis, prenatal diagnosis, tumor genetics and genome research.

[0003] At present, there are dozens of FISH detection items for common blood diseases. In actual clinical testing, due to the high price and variety of probes, FISH testing usually accumulates a certain amount of slides and then performs fluorescent staining experiments uniformly to save costs and avoid waste. Since this technology is to directly observe the sample image under a fluorescent microscope to analyze the results after fluorescent staining the sample, the pre-treatment quality of the sample slide is one of the important factors affecting the accuracy of the analyst's interpretation of the results. In the existing technology, the pre-treatment of sample slides is prone to large errors in subsequent FISH testing due to different operating levels of personnel and lack of standardized operations. Utility Model Content

[0004] In view of this, the purpose of the utility model is to provide a slide FISH pre-processing instrument to solve the problem in the prior art that due to different operating levels of personnel and lack of standardized operation, subsequent FISH detection is prone to large errors.

[0005] The utility model solves the above technical problems through the following technical means: a slide FISH pretreatment instrument comprises a first turntable and a second turntable, the first turntable is evenly distributed with a plurality of quantitative liquid adding components in a circumferential direction, the quantitative liquid adding components comprise a reagent compartment, a liquid adding valve and a liquid adding dropper which are connected in sequence; the second turntable is evenly distributed with a plurality of reaction units with reaction grooves in a circumferential direction, the reaction grooves are inclined, a liquid inlet is arranged at the top of the reaction groove, and a liquid outlet is arranged at the bottom of the reaction groove; the rotation of the first turntable enables any liquid adding dropper to be aligned with the liquid inlet, and the rotation of the second turntable enables any liquid inlet to be aligned with the liquid adding dropper.

[0006] Optionally, the reagent compartments of at least two quantitative liquid adding components are vacuum insulation tanks. The vacuum insulation tanks are provided to maintain the temperature of some reagents.

[0007] Optionally, the rotation of the first rotating disk and the second rotating disk are both driven by a stepper motor, and the rotational angular displacement of the first rotating disk and the second rotating disk is precisely controlled by the stepper motor.

[0008] Optionally, the number of quantitative liquid adding components is nine. It is applicable to FISH detection of samples such as bone marrow or peripheral blood smears.

[0009] Optionally, a flow meter is also provided between the liquid adding valve and the liquid adding dropper. To accurately measure the added reagent.

[0010] Optionally, a liquid outlet valve is connected to the liquid outlet. When adding reagents to the reaction unit through the liquid adding valve, or when the sample slide is undergoing pretreatment, the liquid outlet valve is in a closed state. When drainage is required, the liquid outlet valve is opened to drain the waste liquid into the box.

[0011] Optionally, a waste liquid collection bucket is provided below the outlet of the liquid outlet valve. Used to collect the waste liquid generated during the pretreatment process.

[0012] Advantages of the present utility model:

[0013] By setting the first turntable and the quantitative liquid adding components, the second turntable and the reaction unit, and cooperating with the liquid adding valve and the liquid adding dropper, rotating the first turntable or the second turntable can either sequentially add all reagents to a certain sample slide in the second turntable for pretreatment, or simultaneously perform batch pretreatment operations on all sample slides in the second turntable, taking into account both economy and efficiency; using the present utility model for standardized pretreatment operations makes the pretreatment level of the sample slides stable, thereby reducing the randomness of detection and avoiding the problem of large errors in subsequent FISH detection caused by different levels of personnel operation. Description of the drawings

[0014] Figure 1 is the overall structural schematic diagram of the present utility model;

[0015] Figure 2 is Figure 1 the partial enlarged schematic diagram at M in

[0016] Figure 3 is the sectional view schematic diagram of the reaction unit of the present utility model.

[0017] Wherein, 1 - first turntable, 11 - reagent bin, 12 - liquid adding valve, 13 - liquid adding dropper, 2 - second turntable, 21 - reaction unit, 22 - liquid inlet, 23 - liquid outlet, 3 - waste liquid collection bucket, 4 - sample slide. Specific embodiments

[0018] The following specific embodiments illustrate the implementation manners of the present utility model. Those skilled in the art can understand the advantages and effects of the present utility model from the content disclosed in this specification. It should be noted that the illustrations provided in the following embodiments are only for illustrative purposes, showing only schematic diagrams, not physical diagrams, and should not be construed as limiting the present utility model. To better illustrate the embodiments of the present utility model, some components in the figures will be omitted, enlarged, or reduced, which do not represent the dimensions of the actual products; for those skilled in the art, it is understandable that some well-known structures and their descriptions in the figures may be omitted.

[0019] In the figures of the embodiments of the present utility model, the same or similar reference numerals correspond to the same or similar components. In the description of the present utility model, it should be understood that if there are terms such as "upper", "lower", "left", "right", "front", "rear", etc. indicating the orientation or positional relationship, they are based on the orientation or positional relationship shown in the figures. This is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, the terms describing the positional relationship in the figures are only for illustrative purposes and should not be construed as limiting the present utility model. For those of ordinary skill in the art, the specific meanings of the above terms can be understood according to specific circumstances.

[0020] As Figures 1 - 3 shown, it is the slide FISH pretreatment instrument of the present utility model. As Figure 1 shown, it includes a first turntable 1 and a second turntable 2. The rotations of the first turntable 1 and the second turntable 2 are both driven by a stepper motor, and their structures are the same. For example, the first turntable 1 includes a disk body, the bottom of the disk body is fixedly connected with a rotating shaft, and the end of the rotating shaft is connected to the stepper motor; under the drive of the stepper motor, the first turntable 1 and the second turntable 2 can rotate a corresponding angular displacement precisely. Nine quantitative liquid adding components are circumferentially distributed on the first turntable 1. The quantitative liquid adding component includes a reagent chamber 11, a liquid adding valve 12, and a liquid adding dropper 13 that are connected in sequence. The reagent in the reagent chamber 11 flows out through the liquid adding valve 12 and the liquid adding dropper 13, and the start and stop of liquid addition are controlled by the liquid adding valve 12. A peristaltic pump and a flow meter can also be arranged between the liquid adding valve 12 and the liquid adding dropper 13 to more precisely control the liquid addition amount and avoid the poor flow of the reagent in the pipeline. Nine reaction units 21 for placing sample slides 4 are circumferentially distributed on the second turntable 2. As Figure 3As shown in the figure, a reaction tank is provided at the top of the reaction unit 21. The width of the reaction tank is greater than or equal to the width of the sample slide 4, the depth is slightly greater than the length of the sample slide 4, and the thickness is slightly greater than the thickness of the sample slide 4, so that the sample slide 4 can be freely inserted into the reaction tank. The reaction tank is inclined, the ground surface of the sample slide 4 is placed on the inclined plane, and the light surface of the sample slide 4 is the sample area. A reaction area for the reagent and the sample is formed between the light surface of the sample slide 4 and the inner wall of the reaction tank. An arc-shaped liquid inlet 22 is provided at the top of the reaction tank of the reaction unit 21, and the liquid inlet 22 is communicated with the reaction tank. A liquid outlet 23 is provided at the bottom of the reaction tank of the reaction unit 21, and a liquid outlet valve is connected to the liquid outlet 23. A waste liquid collection bucket 3 with a cover is provided below the outlet of the liquid outlet valve. Nine corresponding liquid discharge holes are provided on the cover, and the liquid discharge holes can be aligned with the liquid outlet 23 or the outlet of the liquid outlet valve. When adding reagents to the reaction unit 21 through the liquid addition valve 12 or when the sample slide 4 is being pretreated, the liquid outlet valve is in a closed state. When liquid discharge is required, the liquid outlet valve is opened to discharge the waste liquid into the waste liquid collection bucket 3.

[0021] The rotation of the first turntable 1 enables any liquid addition dropper 13 to be aligned with the liquid inlet 22; the rotation of the second turntable 2 enables any liquid inlet 22 to be aligned with the liquid addition dropper 13. Thus, the processor can either sequentially add all reagents to a certain sample slide 4 in the second turntable 2 for pretreatment or perform batch pretreatment operations on all sample slides 4 in the second turntable 2 at the same time.

[0022] Since it is necessary to ensure the activity of some reagents, such as pepsin (37 °C), etc., during the pretreatment of the sample slide 4 for FISH detection, in this embodiment, the reagent storage tanks 11 of two quantitative liquid addition components are vacuum heat preservation tanks to maintain the temperature of the reagents in the reagent storage tanks 11.

[0023] It can be understood that existing control elements such as the stepping motor, liquid addition valve 12, peristaltic pump, flow meter, liquid outlet valve, etc. in this embodiment can all be connected to an existing control module through wires, and under the control of the control module, precise control such as timing, quantitative, and constant speed can be achieved to improve the automation level; the control module can be connected to a corresponding digital display operation panel to achieve operation visualization. The above control elements, control module, and digital display panel are all prior arts, and their specific principles and structures will not be elaborated herein.

[0024] The working principle of the present utility model is as follows:

[0025] The sample slide 4 (such as a bone marrow or peripheral blood smear) is first dried and aged overnight at room temperature or aged in an environment of 56 °C for 30 - 60 minutes. Fixative (methanol: glacial acetic acid ratio is 3:1), deionized water, PBS buffer (i.e., phosphate buffered saline, pH value is 7.2), 2×SSC solution (i.e., sodium citrate buffer diluted twice, 37 °C, pH value is 7.0), pepsin solution (37 °C, formulated as 36 ML of deionized water + 4 ML of 0.1 mol / L HCl solution + 0.16 ML of pepsin stock solution), formaldehyde fixative (formulated as 40 ML of PBS buffer with pH value of 7.2 + 1 ML of formaldehyde), 70% ethanol, 85% ethanol, and 100% ethanol are added to the reagent storage. The temperature of the reagents not specified is normal temperature or room temperature.

[0026] Put the dried and aged sample slide 4 into the reaction tank of the reaction unit 21, rotate the first turntable 1, and add reagents to the reaction unit 21 in sequence for pre-treatment of the slide:

[0027] 1. Primary fixation, add fixative, reaction time is 10 minutes;

[0028] 2. Secondary fixation, add fixative, reaction time is 10 minutes;

[0029] 3. Soak in deionized water twice, add deionized water, soaking time is 2 - 3 minutes each time;

[0030] 4. Add PBS buffer, soak for 5 minutes;

[0031] 5. Add 2xSSC buffer (pH 7.0) at 37 °C, balance the slide for 15 minutes;

[0032] 6. Add pepsin at 37 °C, soak for 5 minutes;

[0033] 7. Add 2xSSC buffer (pH 7.0) at 37 °C, balance the slide for 5 minutes;

[0034] 8. Add formaldehyde fixative, soak for 15 minutes;

[0035] 9. Add 70% ethanol, 85% ethanol, and 100% ethanol in sequence, dehydrate for 3 minutes each.

[0036] The sample slide 4 after pre-treatment can be subjected to subsequent detection according to the operation steps of FISH detection. By using the pre-treatment instrument of the present utility model, the first turntable 1 can be rotated to sequentially add all reagents to a certain sample slide 4 in the second turntable 2 for pre-treatment, or the first turntable 1 and the second turntable 2 can be rotated in cooperation to perform batch pre-treatment operations on all sample slides 4 in the second turntable 2. By using the present utility model for standardized pre-treatment operations, the pre-treatment level of the sample slide 4 is stabilized, thereby reducing the randomness of detection and avoiding the problem of large errors in subsequent FISH detection caused by different personnel operation levels.

[0037] The above embodiments are only used to illustrate the technical solutions of the present utility model and not to limit them. Although the present utility model has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present utility model can be modified or equivalently replaced without departing from the purpose and scope of the technical solutions of the present utility model, and they should all be covered within the scope of the claims of the present utility model. The technologies, shapes, and structures not described in detail in the present utility model are all well-known technologies.

Claims

1. Slide FISH pre-processing instrument, characterized by: The invention comprises a first rotating disk (1) and a second rotating disk (2), wherein the first rotating disk (1) is evenly distributed with a plurality of quantitative liquid adding components in the circumferential direction, wherein the quantitative liquid adding components comprise a reagent chamber (11), a liquid adding valve (12) and a liquid adding dropper (13) which are connected in sequence; the second rotating disk (2) is evenly distributed with a plurality of reaction units (21) with reaction grooves in the circumferential direction, wherein the reaction grooves are arranged obliquely, wherein the top of the reaction grooves is provided with a liquid inlet (22), and the bottom of the reaction grooves is provided with a liquid outlet (23); The rotation of the first rotating disk (1) enables any one of the liquid adding droppers (13) to be aligned with the liquid inlet (22), and the rotation of the second rotating disk (2) enables any one of the liquid inlet (22) to be aligned with the liquid adding dropper (13).

2. The slide FISH pre-processing instrument according to claim 1, characterized in that: The reagent chambers (11) of at least two of the quantitative liquid adding components are vacuum heat-insulating tanks.

3. The slide FISH pre-processing instrument according to claim 1, characterized in that: The rotation of the first rotating disk (1) and the second rotating disk (2) are both driven by a stepping motor.

4. The slide FISH pre-processing instrument according to claim 1, characterized in that: The number of the quantitative liquid adding components is nine.

5. The slide FISH pre-processing instrument according to claim 1, characterized in that: A flow meter is also provided between the liquid adding valve (12) and the liquid adding dropper (13).

6. The slide FISH pre-processing instrument according to claim 1, characterized in that: The liquid outlet (23) is connected to a liquid outlet valve.

7. The slide FISH pre-processing instrument according to claim 6, characterized in that: A waste liquid collection bucket (3) is provided below the outlet of the liquid outlet valve.

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