Culture bottle for preparing adipose-derived stem cells

By designing a stratified and closed adipose stem cell preparation flask, the problems of low space utilization and insufficient culture efficiency in the prior art are solved, and efficient adipose stem cell culture is achieved.

CN223047536UActive Publication Date: 2025-07-01山东丽山生物科技有限公司
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Patent Information

Application Number
CN202422102406.2
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-08-29
Publication Date
2025-07-01
Estimated Expiration
2034-08-29

AI Technical Summary

Technical Problem

The existing adipose stem cell preparation culture flasks cannot be used to control culture of multiple groups of adipose stem cells in a separate sealed space, and the space utilization rate is low and the culture efficiency is insufficient.

Method used

A culture bottle for preparing fat stem cells was designed, including the bottle body structure, culture structure, partition structure, transmission structure and connection structure. The partition structure is used to achieve layered and closed culture culture. The transmission structure and connection structure are conveniently installed and removed, and the operation efficiency is improved by using the snap structure.

Benefits of technology

The stratified and closed culture of adipose stem cells is realized, which improves the space utilization and culture efficiency, and enhances the operation convenience of the culture bottle.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model discloses an adipose-derived stem cell preparation culture bottle which comprises a bottle body structure, a bottle cap arranged on the bottle body structure, a bottle pad arranged at one end of the bottle body structure, a culture structure arranged in the bottle body structure, a partition structure arranged on the culture structure, a transmission structure connected to the culture structure in a matched mode, and a connecting structure connected to the transmission structure. And the connecting structure is connected with a buckle structure. The bottle body structure has the beneficial effects that the layered closed culture of adipose-derived stem cells can be realized through the culture structure and the partition structure, the space utilization of the culture bottle can be improved, and meanwhile, the transmission structure and the connecting structure are matched with the buckle structure, so that the culture structure can be mounted, placed and taken out; the culture efficiency is improved.
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Description

Technical Field

[0001] The utility model relates to the technical field of culture flasks, and more specifically, to a culture flask for preparing adipose stem cells. Background Art

[0002] Adipose stem cells are a type of stem cells with multi-directional differentiation potential isolated from adipose tissue. Adipose tissue is rich in reserves in the human body. A large number of adipose stem cells obtained from liposuction have the potential for self-renewal, proliferation, and multi-directional differentiation. They are important research objects and experimental materials in current stem cell clinical research and tissue engineering, and have broad application prospects in the fields of clinical treatment and medical plastic surgery. The existing culture flasks for preparing adipose stem cells are generally monolayer cultures and cannot perform control cultures of multiple groups of adipose stem cells in a separate sealed space. Summary of the Utility Model

[0003] In view of the problems in the related art, the present utility model proposes a culture flask for preparing adipose stem cells to overcome the above-mentioned technical problems existing in the related art.

[0004] To this end, the specific technical solution adopted by the present utility model is as follows:

[0005] A culture flask for preparing adipose stem cells includes a flask body structure, a bottle cap is provided on the flask body structure, a bottle pad is provided at one end of the flask body structure, a culture structure is provided inside the flask body structure, a partition structure is provided on the culture structure, a transmission structure is connected to the culture structure in a matching manner, a connection structure is connected to the transmission structure, and a buckle structure is connected to the connection structure.

[0006] Further, the flask body structure includes a culture flask, a fixing ring, and a guide rod. The fixing ring is fixedly connected to the peripheral side of the top of the culture flask, and the guide rod is fixedly connected to the inner peripheral side of the culture flask. The guide rod is connected to the culture structure in a matching manner.

[0007] Further, the culture structure includes a culture groove, a guide hole, a docking groove, a limiting rod, an introduction groove, a guide groove, and a mounting groove. A guide hole is opened on the outer peripheral side of the culture groove, and the guide hole is matched with the guide rod. A docking groove is opened on the inner ring of the culture groove, a limiting rod is fixedly arranged in the docking groove, an introduction groove is opened on the limiting rod, guide grooves are opened on both sides of the guide hole, and a mounting groove is opened on one side of the guide groove.

[0008] Further, the partition structure includes a partition block, a first inclined groove, a guide block, a guide wheel, and a first spring. A first inclined groove is opened on one side of the partition block, guide blocks are fixedly connected to both ends of the partition block, guide wheels are flatly embedded on both sides of the partition block, a first spring is docked on the side of the partition block away from the first inclined groove, one end of the first spring is fixedly connected in the mounting groove, and the guide block is slidably installed in the guide groove.

[0009] Further, the transmission structure includes a sleeve rod, a transmission rod, a transmission cap, and a second spring. The transmission rod is slidably connected inside the sleeve rod. One end of the transmission rod is fixedly connected to the transmission cap, and a second spring is provided inside the transmission cap. The second spring is installed at one end of the sleeve rod.

[0010] Further, the connection structure includes a docking block, a limiting groove, a through groove, a transmission groove, a reset groove, and an orientation groove. A limiting groove is provided on the periphery of the docking block, and the limiting groove matches the limiting rod. A through groove penetrates through the inner side of the docking block, a transmission groove is provided in the through groove, a reset groove is provided on the transmission groove, and an orientation groove is provided on the reset groove.

[0011] Further, a buckle structure is installed in the transmission groove. The buckle structure includes a transmission block, a third spring, a retaining ring, an orientation block, and a second inclined groove. The third spring is sleeved on the transmission block. The retaining ring is fixedly connected to the transmission block and contacts the third spring. The orientation block is fixedly connected to the retaining ring, and a second inclined groove is provided on one side of the transmission block.

[0012] The beneficial effects of the present utility model are as follows: The bottle body structure of the present utility model can achieve the layered and airtight culture of adipose stem cells through the provided culture structure and partition structure, which can improve the space utilization of the culture bottle. At the same time, the provided transmission structure and connection structure cooperate with the buckle structure to be used for the installation, placement, and removal of the culture structure, improving the culture efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] In order to more clearly illustrate the technical solutions in the embodiments of the present utility model or the prior art, the following will briefly introduce the drawings required for use in the embodiments. Obviously, the following described drawings are only some embodiments of the present utility model. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on these drawings.

[0014] Figure 1 is a schematic diagram of the main structure of a culture bottle for preparing adipose stem cells according to an embodiment of the present utility model;

[0015] Figure 2 is a schematic diagram of the bottle body structure of a culture bottle for preparing adipose stem cells according to an embodiment of the present utility model;

[0016] Figure 3 is a sectional view of the bottle body structure of a culture bottle for preparing adipose stem cells according to an embodiment of the present utility model;

[0017] Figure 4 is a connection diagram of the culture structure of a culture bottle for preparing adipose stem cells according to an embodiment of the present utility model;

[0018] Figure 5It is a sectional view of the culture structure of a culture flask for preparing adipose stem cells according to an embodiment of the present utility model;

[0019] Figure 6 It is a schematic diagram of the partition structure of a culture flask for preparing adipose stem cells according to an embodiment of the present utility model;

[0020] Figure 7 It is a connection diagram of the transmission structure of a culture flask for preparing adipose stem cells according to an embodiment of the present utility model;

[0021] Figure 8 It is a schematic diagram of the transmission structure of a culture flask for preparing adipose stem cells according to an embodiment of the present utility model;

[0022] Figure 9 It is a connection diagram of the connection structure of a culture flask for preparing adipose stem cells according to an embodiment of the present utility model;

[0023] Figure 10 It is a sectional view of the connection structure of a culture flask for preparing adipose stem cells according to an embodiment of the present utility model;

[0024] Figure 11 It is a schematic diagram of the buckle structure of a culture flask for preparing adipose stem cells according to an embodiment of the present utility model.

[0025] In the figure:

[0026] 1. Bottle body structure; 101. Culture flask; 102. Fixed ring; 103. Guide rod; 2. Bottle cap; 3. Bottle gasket; 4. Culture structure; 401. Culture groove; 402. Guide hole; 403. Docking groove; 404. Limit rod; 405. Introduction groove; 406. Guide groove; 407. Installation groove; 5. Partition structure; 501. Partition block; 502. First inclined groove; 503. Guide block; 504. Guide wheel; 505. First spring; 6. Transmission structure; 601. Sleeve rod; 602. Transmission rod; 603. Transmission cap; 604. Second spring; 7. Connection structure; 701. Docking block; 702. Limit groove; 703. Through groove; 704. Transmission groove; 705. Reset groove; 706. Orientation groove; 8. Buckle structure; 801. Transmission block; 802. Third spring; 803. Retaining ring; 804. Orientation block; 805. Second inclined groove. Detailed implementation manners

[0027] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all of the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model.

[0028] According to an embodiment of the present utility model, a culture bottle for preparing adipose stem cells is provided.

[0029] Embodiment 1;

[0030] As Figures 1-11 shown, the culture bottle for preparing adipose stem cells according to an embodiment of the present utility model includes a bottle body structure 1, a bottle cap 2 is provided on the bottle body structure 1, a bottle pad 3 is provided at one end of the bottle body structure 1, a culture structure 4 is provided inside the bottle body structure 1, a partition structure 5 is provided on the culture structure 4, a transmission structure 6 is connected to the culture structure 4 in a matching manner, a connection structure 7 is connected to the transmission structure 6, and a buckle structure 8 is connected to the connection structure 7.

[0031] The culture structure 4 is installed in layers inside the bottle body structure 1. The partition structure 5 makes each layer of the culture structure 4 independent of each other. The transmission structure 6, in cooperation with the connection structure 7 and the buckle structure 8, enables the convenient installation and removal of the culture structure 4. The bottle body structure 1 includes a culture bottle 101, a fixing ring 102, and a guide rod 103. The fixing ring 102 is fixedly connected to the peripheral side of the top of the culture bottle 101. The guide rod 103 is fixedly connected to the inner peripheral side of the culture bottle 101. The guide rod 103 is connected to the culture structure 4 in a matching manner. The guide rod 103 fixedly provided inside the culture bottle 101 is designed in sections for placing the culture structure 4 between the sections. The fixing ring 102 is a threaded ring for threaded connection and fixation with the bottle cap 2.

[0032] The culture structure 4 includes a culture tank 401, a guiding hole 402, a docking groove 403, a limiting rod 404, an introducing groove 405, a guiding groove 406, and a mounting groove 407. A guiding hole 402 is formed on the outer peripheral side of the culture tank 401, and the guiding hole 402 is matched with the guiding rod 103. A docking groove 403 is formed on the inner ring of the culture tank 401, and a limiting rod 404 is fixedly arranged in the docking groove 403. An introducing groove 405 is formed on the limiting rod 404. Guiding grooves 406 are formed on both sides of the guiding hole 402, and a mounting groove 407 is formed on one side of the guiding groove 406. The partition structure 5 includes a partition block 501, a first inclined groove 502, a guiding block 503, a guiding wheel 504, and a first spring 505. A first inclined groove 502 is formed on one side of the partition block 501. Guiding blocks 503 are fixedly connected to both ends of the partition block 501. Guiding wheels 504 are horizontally embedded on both sides of the partition block 501. A first spring 505 is butted against the side of the partition block 501 away from the first inclined groove 502. One end of the first spring 505 is fixedly connected in the mounting groove 407. The guiding block 503 is slidably installed in the guiding groove 406. The culture tank 401 is introduced into the culture bottle 101 under the sliding guidance of the guiding hole 402 and the guiding rod 103. Then, at the fault of the guiding rod 103, the culture tank 401 is rotated through the connecting structure 7 and the transmission structure 6 to be fixedly placed at the fault. When the culture tank 401 moves on the guiding rod 103, since the first inclined groove 502 of the partition block 501 is pushed open under the stressed state, the partition cannot be completed. When it is continuously pushed open at the fault, at this time, under the elastic force of the first spring 505, the two opposite partition blocks 501 are abutted against each other to complete the sealing treatment of the guiding hole 402, preventing good air contact between adjacent culture tanks 401.

[0033] The transmission structure 6 includes a sleeve rod 601, a transmission rod 602, a transmission cap 603, and a second spring 604. The transmission rod 602 is slidably connected inside the sleeve rod 601. One end of the transmission rod 602 is fixedly connected to the transmission cap 603. A second spring 604 is provided inside the transmission cap 603. The second spring 604 is installed at one end of the sleeve rod 601. The connection structure 7 includes a docking block 701, a limiting groove 702, a through groove 703, a transmission groove 704, a reset groove 705, and an orientation groove 706. A limiting groove 702 is provided on the peripheral side of the docking block 701. The limiting groove 702 is matched with the limiting rod 404. A through groove 703 penetrates through the inner side of the docking block 701. A transmission groove 704 is provided in the through groove 703. A reset groove 705 is provided on the transmission groove 704. An orientation groove 706 is provided on the reset groove 705. A buckle structure 8 is installed in the transmission groove 704. The buckle structure 8 includes a transmission block 801, a third spring 802, a retaining ring 803, an orientation block 804, and a second inclined groove 805. The third spring 802 is sleeved on the transmission block 801. The retaining ring 803 is fixedly connected to the transmission block 801. The retaining ring 803 is in contact with the third spring 802. The orientation block 804 is fixedly connected to the retaining ring 803. A second inclined groove 805 is provided on one side of the transmission block 801. One end of the sleeve rod 601 is fixedly connected to the through groove 703 of the docking block 701. The transmission rod 602 is flush with the notch of the through groove 703. When the second spring 604 is compressed by pressing the transmission cap 603, the other end of the transmission rod 602 will protrude, causing the transmission rod 602 to transmit with the second inclined groove 805. The transmission block 801 will move horizontally in the transmission groove 704, and then exit the transmission groove 704, causing one end of the transmission block 801 to enter the guiding groove 405, making the docking block 701 relatively fixed to the culture tank 401, and then it can be rotated and slid up and down, improving the convenience of installation and removal.

[0034] In order to facilitate the understanding of the above technical solutions of the present invention, the working principle or operation method of the present invention in the actual process will be described in detail below.

[0035] The guiding rod 103 fixedly arranged inside the culture bottle 101 is designed in sections and is used to place the culture structure 4 between the sections. The fixing ring 102 is a threaded ring and is used for threaded connection and fixation with the bottle cap 2. The culture groove 401 is guided into the culture bottle 101 under the sliding guiding action of the guiding hole 402 and the guiding rod 103. Then, at the section of the guiding rod 103, the culture groove 401 is rotated through the connecting structure 7 and the transmission structure 6 so that it is fixedly placed at the section. When the culture groove 401 moves on the guiding rod 103, since the first inclined groove 502 of the partition block 501 is pushed open under the stressed state, the partition cannot be completed. When it is continuously pushed open at the section, at this time, under the elastic force of the first spring 505, the two opposite partition blocks 501 are abutted against each other to complete the airtight treatment of the guiding hole 402, preventing good air contact between adjacent culture grooves 401. One end of the sleeve rod 601 is fixedly connected to the through groove 703 of the docking block 701, and the transmission rod 602 is flush with the notch of the through groove 703. When the transmission cap 603 is pressed to compress the second spring 604, the other end of the transmission rod 602 will protrude, causing the transmission rod 602 to transmit with the second inclined groove 805. The transmission block 801 will move horizontally in the transmission groove 704 and then be led out of the transmission groove 704, so that one end of the transmission block 801 enters the introduction groove 405, making the docking block 701 relatively fixed to the culture groove 401, and then it can be rotated and slid up and down, improving the convenience of installation and removal.

[0036] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A culture bottle for preparing adipose stem cells, characterized in that: The invention comprises a bottle body structure (1), wherein a bottle cap (2) is provided on the bottle body structure (1), a bottle pad (3) is provided at one end of the bottle body structure (1), a culture structure (4) is provided inside the bottle body structure (1), a partition structure (5) is provided on the culture structure (4), a transmission structure (6) is matched and connected to the culture structure (4), a connection structure (7) is connected to the transmission structure (6), and a buckle structure (8) is connected to the connection structure (7).

2. The adipose stem cell preparation culture bottle according to claim 1, characterized in that: The bottle body structure (1) comprises a culture bottle (101), a fixing ring (102), and a guide rod (103); the fixing ring (102) is fixedly connected to the top peripheral side of the culture bottle (101); the guide rod (103) is fixedly connected to the inner peripheral side of the culture bottle (101); and the guide rod (103) is matched and connected to the culture structure (4).

3. The adipose stem cell preparation culture bottle according to claim 2, characterized in that: The culture structure (4) comprises a culture groove (401), a guide hole (402), a docking groove (403), a limiting rod (404), an introduction groove (405), a guide groove (406), and an installation groove (407). The outer peripheral side of the culture groove (401) is provided with a guide hole (402), and the guide hole (402) matches the guide rod (103). The inner circle of the culture groove (401) is provided with a docking groove (403), and a limiting rod (404) is fixedly provided in the docking groove (403). The limiting rod (404) is provided with an introduction groove (405), and guide grooves (406) are provided on both sides of the guide hole (402), and an installation groove (407) is provided on one side of the guide groove (406).

4. The adipose stem cell preparation culture bottle according to claim 3, characterized in that: The partition structure (5) comprises a partition block (501), a first inclined groove (502), a guide block (503), a guide wheel (504), and a first spring (505); the first inclined groove (502) is provided on one side of the partition block (501); the guide blocks (503) are fixedly connected at both ends of the partition block (501); the guide wheels (504) are flatly embedded on both sides of the partition block (501); the first spring (505) is butted against a side of the partition block (501) away from the first inclined groove (502); one end of the first spring (505) is fixedly connected to the installation groove (407); and the guide block (503) is slidably installed in the guide groove (406).

5. The adipose stem cell preparation culture bottle according to claim 4, characterized in that: The transmission structure (6) comprises a sleeve rod (601), a transmission rod (602), a transmission cap (603), and a second spring (604); the sleeve rod (601) is slidably connected with the transmission rod (602); one end of the transmission rod (602) is fixedly connected with the transmission cap (603); the transmission cap (603) is provided with a second spring (604); and the second spring (604) is mounted on one end of the sleeve rod (601).

6. The adipose stem cell preparation culture bottle according to claim 5, characterized in that: The connection structure (7) comprises a docking block (701), a limiting groove (702), a through groove (703), a transmission groove (704), a reset groove (705), and an orientation groove (706); the limiting groove (702) is provided on the circumferential side of the docking block (701); the limiting groove (702) matches the limiting rod (404); the through groove (703) is penetrated on the inner side of the docking block (701); a transmission groove (704) is provided in the through groove (703); a reset groove (705) is provided on the transmission groove (704); and an orientation groove (706) is provided on the reset groove (705).

7. The adipose stem cell preparation culture bottle according to claim 6, characterized in that: The buckle structure (8) is installed in the transmission groove (704), and the buckle structure (8) comprises a transmission block (801), a third spring (802), a retaining ring (803), an orientation block (804), and a second inclined groove (805). The transmission block (801) is sleeved with the third spring (802), the retaining ring (803) is fixedly connected to the transmission block (801), the retaining ring (803) is in contact with the third spring (802), the retaining ring (803) is fixedly connected to the orientation block (804), and a second inclined groove (805) is provided on one side of the transmission block (801).