Sleeve type heating and heat preservation device for CTC enrichment dyeing equipment

Abstract

The utility model relates to the technical field of biological medicine, in particular to a sleeve type heating and heat preservation device for CTC enrichment dyeing equipment, which comprises a bottom plate, a plurality of sleeves are fixedly connected on the bottom plate at equal intervals through positioning bolts, a heat preservation groove is sleeved on the peripheral side of the bottom plate, and a cover plate is mounted at the upper end of the heat preservation groove. A plurality of fixing holes allowing the sleeves to penetrate through are formed in the cover plate, the number and the size of the fixing holes are matched with those of the sleeves, and heating belts for heating the sleeves are arranged on the outer side walls of the sleeves in a surrounding mode. The utility model solves the problem that the reaction activity of a reagent is not high due to the lack of a heating and heat preservation component in the CTC cell enrichment dyeing process.

Description

Technical Field

[0001] The utility model relates to the technical field of biomedicine, in particular to a sleeve-type heating and heat preservation device for CTC enrichment and staining equipment. Background Art

[0002] In recent years, some new tumor diagnostic detection technologies have emerged, such as circulating tumor DNA (ctDNA) and circulating tumor cell (CTCs) detection methods, which are called liquid biopsy. CTC detection is easy to obtain, which overcomes the disadvantages of inconvenient sampling of tissue pathology and certain damage to patients. It is currently an advanced method for early screening and diagnosis of malignant tumors. Since the content of CTC in peripheral blood is very low, the capture and identification of CTC in CTC detection is a great challenge.

[0003] Chinese patent CN202321919439.5 discloses "A microfluidic device for circulating tumor cells and an integrated enrichment and staining device", announcement number CN220590059U. This utility model provides a buckle cover with an internal thread and an external thread on the liquid lining, so that the sample injection tube and the filter assembly do not rotate relative to each other during installation, and the liquid sample is evenly distributed on the surface of the filter assembly, thereby improving the filtration and absorption efficiency and saving experimental time.

[0004] During the entire CTC cell enrichment staining experiment, a variety of reagents are used. The reaction activity of these reagents is highest at around 37 degrees. Therefore, the equipment needs to be modified to add a heating component to increase the temperature of the device. Utility Model Content

[0005] In order to solve the above-mentioned problems of the prior art, the utility model provides a sleeve-type heating and heat preservation device for a CTC enrichment and staining device, which solves the problem of low reagent reaction activity caused by lack of heating and heat preservation components during CTC cell enrichment and staining.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a sleeve-type heating and insulation device for CTC enrichment and staining equipment, comprising a base plate, on which a plurality of sleeves are equidistantly fixedly connected by positioning bolts, an insulation groove is sleeved on the peripheral side of the base plate, a cover plate is installed on the upper end of the insulation groove, a plurality of fixing holes for the sleeves to pass through are opened on the cover plate, the fixing holes are matched with the number and size of the sleeves, and a heating belt for heating the sleeve is arranged on the outer wall of the sleeve.

[0007] With the above structural design, the sleeve is heated by the heating belt, thereby increasing the temperature of the filter placed in the sleeve. The operation is simple and the temperature rises quickly, which improves the reaction activity of the reagent and saves experimental time.

[0008] Preferably, the insulation tank is formed by splicing two L-shaped insulation side walls, and the ends of the two insulation side walls are fixed by connecting bolts.

[0009] With the above structural design, the insulation tank is easy to splice and the cover plate can be easily installed in the insulation tank.

[0010] Preferably, a plurality of heating belts are sleeved on the sleeve, and the plurality of heating belts are arranged in parallel, and power lines of the heating belts pass through holes provided on the insulation side wall and out of the insulation tank.

[0011] With the above structural design, multiple heating belts are arranged in parallel, and multiple or one heating belt can be turned on at the same time according to temperature requirements, which is convenient to operate and heats up quickly.

[0012] Preferably, the bottom plate is provided with a through hole located at the center of the sleeve for the drainage pipe of the filter to pass through.

[0013] The above structural design makes it easy to fix the filter in the sleeve, making the filter easier to work.

[0014] Preferably, a heat-insulating layer is provided on the inner side wall of the heat-insulating tank and the lower surface of the cover plate.

[0015] By adopting the above structural design and providing a heat-insulating layer, the heat-insulating effect of the heat-insulating tank can be improved, and the heat exchange efficiency with the outside world can be reduced, thereby reducing the waste of heat energy.

[0016] Compared with the prior art, the beneficial effects of the utility model are:

[0017] The sleeve is heated by a heating belt, thereby increasing the temperature of the filter placed in the sleeve. The operation is simple and the temperature rises quickly, which improves the reaction activity of the reagent and saves experimental time. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the structure of the utility model;

[0019] Figure 2 It is an explosion diagram of the utility model;

[0020] Figure 3 It is a structural schematic diagram of the bottom plate and sleeve of the utility model;

[0021] Figure 4 It is a schematic diagram of the connection between the heating belt and the sleeve of the utility model. DETAILED DESCRIPTION

[0022] See also Figure 1-4The utility model provides a technical solution: a sleeve type heating and heat preservation device for CTC enrichment and staining equipment, comprising a bottom plate 4, the bottom plate 4 is rectangular in shape, a plurality of sleeves 3 are fixedly connected to the bottom plate 4 at equal intervals, a heat preservation tank 1 is sleeved on the periphery of the bottom plate 4, a cover plate 2 is installed on the upper end of the heat preservation tank 1, a plurality of fixing holes 21 for the sleeves 3 to pass through are opened on the cover plate 2, the diameter of the opening of the sleeve 3 is slightly smaller than the diameter of the barrel, so that the cover plate 2 is convenient for clamping the upper end of the sleeve 3, the number and size of the fixing holes 21 are matched with the number and size of the sleeve 3, and a heating belt 8 for heating the sleeve 3 is arranged on the outer wall of the sleeve 3. The sleeve 3 is heated by the heating belt 8, thereby increasing the temperature of the filter 6 placed in the sleeve 3, the operation is simple and the temperature rises quickly, the reaction activity of the reagent is increased, and the experimental time is saved.

[0023] The insulation tank 1 is formed by splicing two L-shaped insulation side walls 11. The insulation tank 1 is in a square tube shape, and the ends of the two insulation side walls 11 are fixed by connecting bolts 9. The insulation tank 1 is easy to splice, and it is convenient to install the cover plate 2 in the insulation tank 1.

[0024] The sleeve 3 is provided with a plurality of heating belts 8, which are arranged in parallel, and the power lines 81 of the heating belts 8 pass through the through holes 5 provided on the insulation side wall 11 and pass out of the insulation tank 1. The plurality of heating belts 8 are arranged in parallel, and a plurality of them or one of them can be turned on at the same time according to the temperature requirements, which is convenient to operate and can heat up quickly.

[0025] The bottom plate 4 is provided with a through hole 7 for the drain pipe of the filter 6 to pass through at the center of the sleeve 3. The through hole 7 is arranged at the bottom of the sleeve 3 to facilitate the fixing of the filter 6 in the sleeve 3 and facilitate the operation of the filter 6.

[0026] The inner wall of the heat preservation tank 1 and the lower surface of the cover plate 2 are both provided with a heat preservation layer 10. The heat preservation layer 10 is made of asbestos material. The heat preservation layer 10 can make the heat preservation tank 1 have a better heat preservation effect, reduce the heat exchange efficiency with the outside, and thus reduce the waste of heat energy.

[0027] Working principle: First, the operator fixes an equal number of sleeves 3 on the base plate 4 through positioning bolts 41 according to the number of filters 6 required for the experiment. After the sleeves 3 are installed, the filters 6 are placed into the sleeves 3 in turn, and the drainage pipes of the filters 6 pass through the through holes 7 opened on the base plate 4. Then, a plurality of heating belts 8 are arranged between the sleeves 3, and the heating belts 8 are close to the outer wall of the sleeves 3. The cover plate 2 is placed on the upper part of the sleeve 3, and the upper end of the sleeve 3 passes through the fixing hole 21 on the cover plate 2. Then, the sleeve 3 is surrounded by two insulating side walls 11, and the two ends of the insulating side walls are connected by connecting bolts 9. The sleeve 3 is heated by the arranged heating belts 8, thereby increasing the temperature of the filters 6 placed in the sleeve 3. The operation is simple and the heating is fast, which improves the reaction activity of the reagents and saves experimental time.

[0028] The structures, proportions, sizes, etc. illustrated in the drawings of this specification are only used to match the contents disclosed in the specification for people familiar with this technology to understand and read, and are not used to limit the limiting conditions for the implementation of the utility model, so they have no substantial technical significance. Any modification of the structure, change of the proportion relationship or adjustment of the size, without affecting the effect and purpose that can be achieved by the utility model, should still fall within the scope of the technical content disclosed by the utility model. At the same time, the terms such as "upper", "lower", "left", "right", "middle" and "one" quoted in this specification are only for the convenience of description, and are not used to limit the scope of the implementation of the utility model. The change or adjustment of their relative relationship should also be regarded as the scope of the implementation of the utility model without substantial change of the technical content.

[0029] The present invention is described above with reference to the preferred embodiments, but the protection scope of the present invention is not limited thereto, and all technical solutions falling within the scope of the claims are within the protection scope of the present invention. Various improvements may be made to the present invention and parts thereof may be replaced with equivalents without departing from the scope of the present invention. In particular, the various technical features mentioned in the various embodiments may be combined in any manner as long as there is no structural conflict.

Claims

1. A sleeve type heating and heat preservation device for CTC enrichment and staining equipment, characterized in that: The invention comprises a bottom plate (4), on which a plurality of sleeves (3) are fixedly connected at equal intervals, a heat preservation groove (1) is sleeved on the circumferential side of the bottom plate (4), a cover plate (2) is installed on the upper end of the heat preservation groove (1), and a plurality of fixing holes (21) are provided on the cover plate (2) for the sleeves (3) to pass through, the fixing holes (21) being matched with the number and size of the sleeves (3), and a heating belt (8) is provided on the outer wall of the sleeve (3) for heating the sleeve (3).

2. The sleeve-type heating and heat preservation device for CTC enrichment and staining equipment according to claim 1, characterized in that: The heat-insulating tank (1) is formed by splicing two L-shaped heat-insulating side walls (11), and the ends of the two heat-insulating side walls (11) are fixed by connecting bolts (9).

3. A sleeve-type heating and heat preservation device for CTC enrichment and staining equipment according to claim 2, characterized in that: A plurality of heating belts (8) are sleeved on the sleeve (3), and the plurality of heating belts (8) are arranged in parallel. The power lines (81) of the heating belts (8) pass through the through holes (5) provided on the heat-insulating side wall (11) and out of the heat-insulating tank (1).

4. The sleeve-type heating and heat preservation device for CTC enrichment and staining equipment according to claim 1, characterized in that: The bottom plate (4) is provided with a through hole (7) located at the center of the sleeve (3) for the drainage pipe of the filter (6) to pass through.

5. The sleeve-type heating and heat preservation device for CTC enrichment and staining equipment according to claim 1, characterized in that: A heat-insulating layer (10) is provided on the inner side wall of the heat-insulating tank (1) and the lower surface of the cover plate (2).

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