Fan-shaped disc

Abstract

The utility model discloses a fan-shaped disc which comprises a disc body with a fan-shaped structure, and a sample adding groove, a separation groove, a quantitative unit, at least one detection groove and at least one second waste liquid groove which are sequentially arranged on the disc body from the rotating center of the disc body to the peripheral direction, the sample adding tank is connected with the separating tank, the separating tank is connected with the quantifying unit through the first micro-channel, the quantifying unit is connected with the detecting tank through the second micro-channel, and the detecting tank is connected with the second waste liquid tank through the third micro-channel. The fan-shaped disc adopts a fan-shaped structure, and a single disc can be matched with a small number of detection items, so that the waste of the disc is reduced; and the disc integrates separation, quantification, cleaning and detection of samples, so that the detection efficiency can be greatly improved.

Description

Technical Field

[0001] The utility model relates to the technical field of biochemical detection, in particular to a sector-shaped disc. Background Art

[0002] Centrifugation technology is one of the most commonly used methods for separating proteins, enzymes, nucleic acids, and cell subcomponents, and is also a commonly used method for separation, purification, or clarification in biochemical laboratories. Centrifugation detection technology usually uses a centrifugal disc as a carrier, and realizes the sequential separation and detection of sample liquids by controlling the disc rotation speed. Point-of-care testing (POCT) refers to clinical testing and bedside testing performed beside the patient, which can quickly obtain test results by omitting the complex processing procedures of specimens during laboratory testing. Existing discs are generally in the form of a whole disc, and multiple items can be detected simultaneously on the disc. Such a disc can achieve high-throughput and rapid detection. However, when facing fewer detection items, using such a disc will cause a large amount of waste, which is not conducive to controlling the detection cost. Summary of the Invention

[0003] In order to overcome the deficiencies of the prior art, the purpose of the utility model is to provide a sector-shaped disc. The sector-shaped disc adopts a sector structure, and a single disc can match a relatively small number of detection items, which is beneficial to reducing the waste of the disc.

[0004] To solve the above problems, the following solutions are adopted in the utility model:

[0005] The utility model includes a sector-shaped disc, which comprises a disc body with a sector structure, and a sample addition groove, a separation groove, a quantification unit, at least one detection groove, and at least one second waste liquid groove arranged on the disc body in sequence along the rotation center of the disc body towards the peripheral direction; the sample addition groove is connected to the separation groove, the separation groove is connected to the quantification unit through a first microchannel, the quantification unit is connected to the detection groove through a second microchannel, and the detection groove is connected to the second waste liquid groove through a third microchannel.

[0006] Preferably, it further includes at least one injection groove, the injection groove is connected to the detection groove, and the injection groove is located between the quantification unit and the detection groove.

[0007] Preferably, the quantification unit includes a shunt channel, a first waste liquid groove, and at least one quantification groove. One end of the shunt channel is connected to the separation groove through a first microchannel, the other end is connected to the first waste liquid groove, the quantification groove is connected to the shunt channel, and the quantification groove is connected to the detection groove through a second microchannel.

[0008] Preferably, a first ventilation hole is connected to the first waste liquid groove.

[0009] Preferably, a second ventilation hole is connected to the second waste liquid groove.

[0010] Preferably, a positioning groove is provided at the center of the disc body, and a first positioning groove is provided on its outer periphery.

[0011] Preferably, the central angle of the disc body is any one of 30°, 45°, 60°, 90°, 120°, and 180°.

[0012] Preferably, a second positioning groove is further provided on the outer periphery, and the first positioning groove and the second positioning groove have different sizes.

[0013] Compared with the prior art, the beneficial effects of the present utility model are as follows:

[0014] In the present utility model, the disc is of a sector structure. A single disc can match a relatively small number of detection items. By combining multiple discs into a circular disc, the detection of a relatively small number of detection items of multiple detection objects can be realized simultaneously, greatly improving the utilization rate of the discs and reducing the waste of discs. Moreover, the disc integrates the separation, quantification, cleaning, and detection of samples, which can greatly improve the detection efficiency. Description of the Drawings

[0015] Figure 1 It is a schematic structural diagram of the sector disc in a preferred embodiment of the present utility model;

[0016] Figures 2 - 4 It is a schematic structural diagram of the sector disc in different embodiments;

[0017] Among them, the description of the reference numerals: 1 is the disc body, 10 is the positioning groove, 11 is the first positioning groove, 12 is the second positioning groove, 13 is the auxiliary plate, 21 is the sample addition groove, 22 is the separation groove, 23 is the first microchannel, 241 is the shunt channel, 242 is the first waste liquid tank, 243 is the quantification tank, 244 is the first ventilation hole, 25 is the second microchannel, 26 is the injection groove, 27 is the detection groove, 28 is the third microchannel, 29 is the second waste liquid tank, 291 is the second ventilation hole. Detailed Description of the Embodiment

[0018] The present utility model will be further described in detail below with reference to the accompanying drawings.

[0019] Refer to Figure 1, which is a schematic structural diagram of a sector disk in a preferred embodiment of the present utility model. The sector disk includes a disk body 1 with a sector structure, and a sample adding groove 21 for adding samples, a separation groove 22 for separating samples, a quantitative unit for quantitatively shunting the components to be measured, at least one detection groove 27 for mixing, incubating / cleaning and detecting, and at least one second waste liquid groove 29 for collecting waste liquid, which are sequentially arranged on the disk body 1 along the rotation center of the disk body 1 towards the outer circumference direction; the sample adding groove 21 is connected to the separation groove 22, the separation groove 22 is connected to the quantitative unit through a first microchannel 23, the separation of the sample in the separation groove 22 is achieved through the first microchannel 23, and the flow of the components to be measured in the sample towards the quantitative unit is controlled; the quantitative unit is connected to the detection groove 27 through a second microchannel 25, and the quantitatively controlled components to be measured are controlled to flow into the detection groove 27 through the second microchannel 25; the detection groove 27 is connected to the second waste liquid groove 29 through a third microchannel 28, and the flow of the waste liquid into the second waste liquid groove 29 is controlled through the third microchannel 28. The disk body adopts a sector structure. A single disk can match a relatively small number of detection items. By combining multiple disks into a circular disk, the detection of a relatively small number of detection items for multiple detection objects can be achieved simultaneously, greatly improving the utilization rate of the disk and reducing the waste of the disk; moreover, this disk integrates the separation, quantification, cleaning and detection of samples, which can greatly improve the detection efficiency.

[0020] A semi-permeable membrane can be arranged in the above-mentioned first microchannel 23 to separate the sample in the separation groove 22 during the centrifugation process, and the components to be measured enter the quantitative unit through the first microchannel; a microfluidic valve can be arranged in the second microchannel 25 to strengthen the control of the flow direction of the components to be measured during centrifugation; a microfluidic valve can be arranged in the third microchannel 28 to strengthen the control of the flow direction of the waste liquid during centrifugation.

[0021] Specifically, it further includes at least one injection groove 26 for injecting reaction liquid into the detection groove 27. The injection groove 26 is connected to the detection groove 27, and the injection groove 26 is located at a position between the quantitative unit and the detection groove 27, that is, the distance from its rotation center of the disk body 1 is between the distance from the quantitative unit to the rotation center of the disk body 1 and the distance from the detection groove 27 to the rotation center of the disk body 1.

[0022] Specifically, referring again to Figure 1, the quantification unit includes a shunt channel 241 for shunting the component to be measured, a first waste liquid tank 242 for receiving the excess component to be measured, and at least one quantification tank 243 for quantitatively measuring the component to be measured entering the detection tank 27. One end of the shunt channel 241 is connected to the separation tank 22 through the first microchannel 23, and the other end is connected to the first waste liquid tank 242. The quantification tank 243 is connected to the shunt channel 241, and the quantification tank 243 is connected to the detection tank 27 through the second microchannel 25. Further, the shunt channel 241 is generally arc-shaped, and the distance from the end close to the first microchannel 23 to the rotation center of the disk body 1 is not greater than the distance from the end close to the first waste liquid tank 242 to the rotation center of the disk body 1, which is convenient for the excess component to be measured to be collected into the first waste liquid tank 242.

[0023] Specifically, a first ventilation hole 244 is connected to the first waste liquid tank 242, and a second ventilation hole 291 is connected to the second waste liquid tank 29. The ventilation hole structure can adjust the air pressure in the disk body 1, which is convenient for the flow of the component to be measured or the waste liquid in the disk body 1 and facilitates their collection.

[0024] A positioning groove 10 is provided at the center of the disk body 1. In a specific embodiment, the positioning groove 10 is preferably a W-shaped or wavy structure, which is convenient for positioning during the installation of the disk.

[0025] In another specific embodiment, a first positioning groove 11 and a second positioning groove 12 are provided on the outer periphery of the disk body 1. The sizes of the first positioning groove 11 and the second positioning groove 12 are different and are used for positioning during the installation of the disk. At this time, the positioning groove 10 is preferably set to be arc-shaped or U-shaped, which is convenient for installation and disassembly.

[0026] Specifically, an auxiliary plate 13 for facilitating the disassembly of the disk is also provided on the outer periphery of the disk body 1.

[0027] Refer to Figure 2 , in a specific embodiment, the central angle of the disk body 1 is 60°. Correspondingly, the detection tank 27 is provided in three groups, and the detection of three detection items for one detection object can be realized.

[0028] Refer to Figure 3 , in a specific embodiment, the central angle of the disk body 1 is 90°. Correspondingly, the detection tank 27 is provided in four groups, and the detection of four detection items for one detection object can be realized.

[0029] Refer to Figure 4 , in a specific embodiment, the central angle of the disk body 1 is 120°. Correspondingly, the detection tank 27 is provided in five groups, and the detection of five detection items for one detection object can be realized.

[0030] In other specific embodiments, the central angle of the disk body 1 can also be selected from any of the angles of 30°, 45°, or 180°. Correspondingly, the number of detection grooves 27 on the disk body 1 can be adjusted according to the number of detection targets and the size of the detection amount.

[0031] The following further elaborates on Figure 1 the principle of using the detection disk in

[0032] First step, add a sample into the sample addition groove of the sector disk.

[0033] Second step, through centrifugation, the sample enters the separation groove, and the component to be detected in the sample in the separation groove is separated into the shunt flow channel through the first microchannel.

[0034] Third step, continue centrifugation. After the component to be detected fills the quantitative groove, the excess component to be detected enters the first waste liquid groove.

[0035] Fourth step, adjust the centrifugation speed so that the component to be detected in the quantitative groove breaks through the second microchannel and enters the detection groove.

[0036] Fifth step, stop centrifugation, add a reaction solution into the injection groove, and centrifuge again so that the reaction solution enters the detection groove. By rotating forward and backward and performing treatments such as heating on the detection groove, the component to be detected and the reaction solution are mixed and incubated to obtain a mixed solution containing an intermediate.

[0037] Sixth step, adjust the centrifugation speed so that the waste liquid in the detection groove breaks through the third microchannel and enters the second waste liquid groove, and an intermediate containing impurities is obtained in the detection groove; stop centrifugation, add a cleaning solution into the injection groove, and centrifuge again so that the cleaning solution enters the detection groove. By rotating forward and backward, thorough cleaning of the intermediate by the cleaning solution is achieved; adjust the centrifugation speed so that the cleaning solution containing impurities breaks through the third microchannel and enters the second waste liquid groove, and a high-purity intermediate is obtained in the detection groove.

[0038] Seventh step, stop centrifugation, add a luminescent substrate into the injection groove, and centrifuge again so that the luminescent substrate enters the detection groove. By rotating forward and backward and performing treatments such as heating on the detection groove, the intermediate and the luminescent substrate are mixed and reacted. After the reaction ends, detection and analysis can be performed through a detection device.

[0039] It can be understood that Figures 2 - 4 the detection disk in

[0040] This disc is particularly suitable for immunoassay chemical detection items, and can be used for fully automated detection of various indicators in body fluids such as urine, saliva, semen, spinal cord or amniotic fluid of humans or animals. It can also be used in the field of food safety to detect toxic and harmful substances, bacteria or viruses in food. It can also be used in the pharmaceutical and chemical industries to detect various pharmaceutical ingredients and chemical products.

[0041] The embodiments of this specific implementation manner are all preferred embodiments of the present invention, and do not limit the protection scope of the present invention accordingly. Therefore, all equivalent changes made according to the structure, shape and principle of the present invention should be covered within the protection scope of the present invention.

Claims

1. A sector-shaped disc, characterized in that: The invention comprises a disc body with a fan-shaped structure, and a sample loading slot, a separation slot, a quantitative unit, at least one detection slot and at least one second waste liquid slot which are arranged on the disc body in sequence from the rotation center of the disc body to the peripheral direction; the sample loading slot is connected to the separation slot, the separation slot is connected to the quantitative unit through a first microfluidic channel, the quantitative unit is connected to the detection slot through a second microfluidic channel, and the detection slot is connected to the second waste liquid slot through a third microfluidic channel.

2. The sector disk according to claim 1, characterized in that: It also includes at least one injection slot, which is connected to the detection slot and is located between the quantitative unit and the detection slot.

3. The sector disk according to claim 1, characterized in that: The quantitative unit includes a shunt channel, a first waste liquid tank and at least a certain amount tank. One end of the shunt channel is connected to the separation tank through the first microchannel, and the other end is connected to the first waste liquid tank. The quantitative tank is connected to the shunt channel, and the quantitative tank is connected to the detection tank through the second microchannel.

4. The sector disk according to claim 3, characterized in that: The first waste liquid tank is connected with a first vent hole.

5. The sector disk according to any one of claims 1 to 4, characterized in that: The second waste liquid tank is connected with a second vent hole.

6. The sector disk according to claim 5, characterized in that: The center of the disk is provided with a positioning groove, and the outer circumference thereof is provided with a first positioning groove.

7. The sector disk according to claim 6, characterized in that: The central angle of the disk is any one of 30°, 45°, 60°, 90°, 120°, and 180°.

8. The sector disk according to claim 6, characterized in that: A second positioning groove is also arranged on the outer periphery, and the sizes of the first positioning groove and the second positioning groove are different.

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