Separation tube for separating and extracting immune cells

Abstract

The utility model relates to the technical field of separation tubes, and discloses a separation tube for separating and extracting immune cells, which comprises a separation tube, and a fixing mechanism is arranged on the surface of the separation tube. After blood is poured into a separation tube, a cover plate is placed above the separation tube, at the moment, a connecting cylinder and a screw block can be inserted into a threaded fixing cylinder in a groove, then the connecting cylinder is rotated, and the connecting cylinder can move downwards, so that the cover plate and a circular rubber block are driven to move downwards, and the bottom of the circular rubber block is tightly attached to the top end of the separation tube; the circular rubber block is arranged at the opening in the top of the separation tube, so that cell blood in the separation tube cannot be thrown out of the separation tube when being separated, the nesting sleeve is placed in the separation sleeve, the sliding block is inserted into the T-shaped groove, then separation liquid, blood and the like are poured into the separation tube in sequence and then are separated, the sliding block can be in the T-shaped groove in the separation process, and the cell blood in the separation tube cannot be thrown out of the separation tube. The T-shaped groove is used for limiting the sliding block, so that the nesting sleeve does not shake in the separation pipe during swinging.

Description

Technical Field

[0001] The utility model relates to the technical field of separation tubes, and specifically relates to a separation tube for separating and extracting immune cells. Background Art

[0002] A separation tube for separating and extracting immune cells (especially peripheral blood mononuclear cells, PBMC) usually combines a specific separation liquid (such as lymphocyte separation liquid, density gradient centrifugation liquid) and an optimized design to simplify the experimental process and improve the separation efficiency and cell purity.

[0003] According to the cell separation tube with application number 202123110108.0 for rapidly separating and extracting PBMC, which belongs to the technical field of cell separation tubes, the key points of its technical solution include a separation tube body, an inner fixed connection of the separation tube body is provided with a sieve plate cylinder support, the top of the sieve plate cylinder support is fixedly connected with a sieve plate reinforcement rib, the top of the sieve plate reinforcement rib is fixedly connected with a separation sieve plate, a sieve plate bottom liquid injection hole is opened at the bottom of the separation sieve plate, the bottom of the separation tube body is fixedly connected with a separation tube bottom, and the outside of the top of the separation tube body is movably connected with a separation tube cover.

[0004] Due to the design of the isolation sieve plate, this device can quickly add a blood sample to the liquid surface of the separation liquid and prevent the mixing of the blood layer and the separation liquid. When centrifuging, there is no need to limit the centrifuge brake; after centrifugation, the separated PBMCs can be directly and easily poured into a new centrifuge tube, saving time and being convenient to operate, and realizing the convenient, fast and efficient extraction of PBMC in a short time. However, when separating blood, the top cover of this device will separate from the separation tube, thus affecting the blood separation. Content of the Utility Model

[0005] The purpose of the utility model is to provide a separation tube for separating and extracting immune cells to solve the problems put forward in the above background art.

[0006] To achieve the above purpose, the utility model provides the following technical solution: a separation tube for separating and extracting immune cells, including a separation tube, a fixing mechanism is arranged on the surface of the separation tube, and a limiting mechanism is arranged on the inner wall of the separation tube;

[0007] The fixing mechanism includes a cover plate, the cover plate is arranged at the top end of the separation tank, a circular rubber block is fixedly connected to the bottom of the cover plate, a T-shaped ring groove is opened at the bottom of the cover plate, a ring is rotatably connected to the T-shaped ring groove, a connecting cylinder is fixedly connected to the bottom of the ring, an internal thread fixing cylinder is fixedly connected to the surface of the separation tube near the top end, and a screw block is in internal thread connection with the internal thread fixing cylinder.

[0008] Preferably, the bottom of the cover plate fits with the top end of the separation tube.

[0009] Preferably, the diameter of the circular rubber block is larger than that of the separation tube, facilitating the circular rubber block to cover the top end of the separation tube.

[0010] Preferably, the surface of the connecting cylinder is rotatably connected in the T-shaped ring groove.

[0011] Preferably, the other side of the screw block is fixedly connected to the bottom end of the surface of the connecting cylinder.

[0012] Preferably, the limiting mechanism includes a fixing ring. The fixing ring is fixedly connected to the inner wall of the separation tube near the bottom end. The top of the fixing ring is provided with T-shaped grooves in a circular array. A slider is slidably connected in the T-shaped groove, and the other end of the slider is fixedly connected with a nest.

[0013] Preferably, the size of the inside of the T-shaped groove is larger than that of the slider, facilitating the slider to slide in the T-shaped groove.

[0014] Compared with the prior art, the present utility model provides a separation tube for separating and extracting immune cells, having the following beneficial effects:

[0015] For this separation tube for separating and extracting immune cells, after pouring blood into the separation tube and placing the cover plate above the separation tube, at this time, the connecting cylinder and the screw block will be threadedly fixed in the threaded fixing cylinder in the plug-in groove. Then rotate the connecting cylinder, and the connecting cylinder will move downward, thereby driving the cover plate and the circular rubber block to move downward, making the bottom of the circular rubber block tightly fit the top end of the separation tube, so that the circular rubber block seals the opening at the top of the separation tube, preventing the cell blood in the separation tube from splashing out during the separation process.

[0016] For this separation tube for separating and extracting immune cells, with the limiting mechanism, place the nest in the separation sleeve, insert the slider into the T-shaped groove, then pour the separation liquid, blood, etc. into the separation tube in an orderly manner, and then separate them. During the separation process, the slider will be in the T-shaped groove, and the T-shaped groove limits the slider, further limiting the nest, so that the nest will not shake randomly in the separation tube during the shaking process. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the technical solutions in the embodiments of the present utility model, the following will briefly introduce the drawings required for the description of the embodiments. Obviously, the following drawings are only some embodiments of the present utility model. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts:

[0018] Figure 1 It is a three-dimensional structural schematic diagram of the present utility model;

[0019] Figure 2This is the front sectional view schematic diagram of the separation tube of the structure of the present utility model;

[0020] Figure 3 This is the schematic diagram of the fixing mechanism of the structure of the present utility model;

[0021] Figure 4 This is the schematic diagram of the limiting mechanism of the structure of the present utility model.

[0022] In the figure: 1. Separation tube; 2. Fixing mechanism; 21. Cover plate; 22. Circular rubber block; 23. T-shaped ring groove; 24. Ring; 25. Connecting cylinder; 26. Internal thread fixing cylinder; 27. Screw block; 3. Limiting mechanism; 31. Fixed ring; 32. T-shaped groove; 33. Slide block; 34. Nesting. Specific implementation mode

[0023] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without making creative efforts shall fall within the protection scope of the present utility model.

[0024] In the present utility model, unless otherwise clearly defined and limited, terms such as "installation", "connection", "connection", "fixation" and other terms should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or integrated; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two components or the interaction relationship between two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific situations.

[0025] The present utility model provides the following technical solutions: Embodiment 1

[0026] Please refer to Figures 1-3 , the present utility model provides a technical solution: a separation and extraction separation tube 1 for immune cells, including a separation tube 1, a fixing mechanism 2 is arranged on the surface of the separation tube 1, and a limiting mechanism 3 is arranged on the inner wall of the separation tube 1;

[0027] The fixing mechanism 2 includes a cover plate 21, the cover plate 21 is arranged at the top of the separation tank, a circular rubber block 22 is fixedly connected to the bottom of the cover plate 21, a T-shaped ring groove 23 is opened at the bottom of the cover plate 21, a ring 24 is rotatably connected to the T-shaped ring groove 23, a connecting cylinder 25 is fixedly connected to the bottom of the ring 24, an internal thread fixing cylinder 26 is fixedly connected to the surface of the separation tube 1 near the top, and a screw block 27 is connected to the internal thread of the internal thread fixing cylinder 26.

[0028] The bottom of the cover plate 21 fits against the top end of the separation tube 1.

[0029] The diameter of the circular rubber block 22 is larger than that of the separation tube 1, facilitating the circular rubber block 22 to cover the top end of the separation tube 1.

[0030] The surface of the connecting cylinder 25 is rotatably connected within the T-shaped ring groove 23.

[0031] The other side of the screw block 27 is fixedly connected to the bottom end of the surface of the connecting cylinder 25. Embodiment 2

[0032] Please refer to Figure 4 and, on the basis of Embodiment 1, further obtain the limiting mechanism 3.

[0033] The limiting mechanism 3 includes a fixed ring 31, which is fixedly connected to the inner wall of the separation tube 1 near the bottom end. The top of the fixed ring 31 is provided with T-shaped grooves 32 in a circular array. A slider 33 is slidably connected within the T-shaped groove 32, and the other end of the slider 33 is fixedly connected to a sleeve 34.

[0034] The internal dimension of the T-shaped groove 32 is larger than that of the slider 33, facilitating the slider 33 to slide within the T-shaped groove 32.

[0035] During the actual operation process, when this device is used, after pouring the blood into the separation tube 1, place the cover plate 21 above the separation tube 1. At this time, the connecting cylinder 25 and the screw block 27 will be threadedly fixed within the threaded fixing cylinder 26 in the insertion slot. Then rotate the connecting cylinder 25, and the connecting cylinder 25 will move downward, thereby driving the cover plate 21 and the circular rubber block 22 to move downward, making the bottom of the circular rubber block 22 tightly fit against the top end of the separation tube 1, covering the opening at the top of the separation tube 1, so that the cellular blood in the separation tube 1 will not be thrown out during the separation process;

[0036] Place the sleeve 34 within the separation sleeve, insert the slider 33 into the T-shaped groove 32. Then pour the separation liquid, blood, etc. into the separation tube 1 in an orderly manner and perform separation on it. During the separation process, the slider 33 will be within the T-shaped groove 32, and the T-shaped groove 32 limits the slider 33, further limiting the sleeve 34, so that the sleeve 34 will not wobble randomly within the separation tube 1 during the swinging process.

[0037] It should be noted that in this text, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprising", "including" or any other variant thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device comprising a series of elements not only includes those elements but also includes other elements not expressly listed, or also includes elements inherent to such process, method, article or device.

[0038] Finally, it should be noted that the above are only the preferred embodiments of the present utility model and are not used to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present utility model shall be included within the protection scope of the present utility model.

Claims

1. A separation tube (1) for separating and extracting immune cells, comprising a separation tube (1), characterized in that: A fixing mechanism (2) is arranged on the surface of the separation tube (1), and a limiting mechanism (3) is arranged on the inner wall of the separation tube (1); The fixing mechanism (2) includes a cover plate (21), the cover plate (21) is arranged at the top end of the separation tank, a circular rubber block (22) is fixedly connected to the bottom of the cover plate (21), a T-shaped ring groove (23) is opened at the bottom of the cover plate (21), a ring (24) is rotatably connected to the T-shaped ring groove (23), a connecting cylinder (25) is fixedly connected to the bottom of the ring (24), an internally threaded fixing cylinder (26) is fixedly connected to the surface of the separation tube (1) near the top end, and a screw block (27) is connected to the internally threaded fixing cylinder (26) by internal threads.

2. The isolation and extraction separation tube (1) of an immune cell according to claim 1, characterized in that: The bottom of the cover plate (21) is attached to the top end of the separation tube (1).

3. The separation tube (1) for separating and extracting immune cells according to claim 1, characterized in that: The diameter of the circular rubber block (22) is larger than the diameter of the separation tube (1).

4. A separation tube (1) for separating and extracting immune cells according to claim 1, characterized in that: The surface of the connecting cylinder (25) is rotatably connected in the T-shaped ring groove (23).

5. A separation tube (1) for separating and extracting immune cells according to claim 1, characterized in that: The other side of the screw block (27) is fixedly connected to the bottom end of the surface of the connecting cylinder (25).

6. The isolation tube (1) for separating and extracting immune cells according to claim 1, wherein: The limiting mechanism (3) includes a fixing ring (31), the fixing ring (31) is fixedly connected to the inner wall of the separation tube (1) near the bottom end, a T-shaped groove (32) is arranged in a circular array at the top of the fixing ring (31), a slider (33) is slidably connected in the T-shaped groove (32), and the other end of the slider (33) is fixedly connected to a nest (34).

7. The isolation tube (1) for separating and extracting immune cells according to claim 6, characterized in that: The size of the inside of the T-shaped groove (32) is larger than the size of the slider (33).

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