Self-cleaning type automatic stirring bottle for culturing intestinal cells

Abstract

The utility model relates to a self-cleaning type automatic stirring bottle, in particular to a self-cleaning type automatic stirring bottle for culturing intestinal cells. The self-cleaning type automatic stirring bottle comprises a bottle body, a convex ring, a sealing cover, a bottle seat, a motor, a clamping block and the like, the motor is fixedly connected in the bottle seat, the clamping block is fixedly connected to an output shaft of the motor, the bottle body is placed on the top of the bottle seat, the convex ring is fixedly connected to the inner wall of the bottle body, and the sealing cover is connected to the upper portion of the bottle body in a threaded mode. The stirring rod is driven by the motor to rotate, the stirring rod rotates to stir a cleaning agent and clear water, the cleaning agent is scattered and foamed, residues on the inner wall of a bottle body are repeatedly scraped, deep cleaning of the cell culture bottle is achieved, the cell culture bottle is repeatedly used, the utilization rate of the cell culture bottle is increased, and the production cost is reduced. The cultivation consumables and cost are reduced, and environmental protection is facilitated.

Description

Technical Field

[0001] The utility model relates to a self-cleaning automatic stirring bottle, in particular to a self-cleaning automatic stirring bottle for intestinal cell cultivation. Background Art

[0002] Intestinal cell cultivation, also known as intestinal cell cloning technology, refers to a method of simulating the in vivo environment (sterile, appropriate temperature, pH value, certain nutritional conditions, etc.) in vitro to enable it to survive, grow, reproduce and maintain the main structure and functions.

[0003] Currently, the cell culture bottles on the market are mainly made of glass or plastic. Such consumables are different from ordinary medical plastic bottles and are relatively expensive. If the cell culture bottles used only once are discarded, it will increase the expenditure cost of cell cultivation and is not conducive to environmental protection. If the cell culture bottles are to be reused, they need to be stirred and cleaned repeatedly with clean water, cleaning agents, double-distilled water, etc. Moreover, the time for each stirring and cleaning process is up to more than 30 minutes. At the same time, it is necessary to manually brush the inner wall of the bottle repeatedly with other tools such as brushes. This stirring and cleaning method not only takes a long time and has low efficiency, but also may have the phenomenon that the cell culture bottles that have previously cultivated cells are not cleaned thoroughly.

[0004] Therefore, it is necessary to design a self-cleaning automatic stirring bottle for intestinal cell cultivation to solve the above technical problems. Summary of the Utility Model

[0005] In order to overcome the problems that manual stirring and cleaning of cell culture bottles not only take a long time and have low efficiency, but also may have the phenomenon that the cell culture bottles that have previously cultivated cells are not cleaned thoroughly, the utility model provides a self-cleaning automatic stirring bottle for intestinal cell cultivation.

[0006] The technical solution is: a self-cleaning automatic stirring bottle for intestinal cell cultivation, which includes a bottle body, a convex ring, a sealing cover, a bottle seat, a motor, a clamping block, a clamping connection block, a stirring rod and a controller. A motor is fixedly connected inside the bottle seat, a clamping block is fixedly connected to the output shaft of the motor, the bottle body is placed on the top of the bottle seat, a controller is fixedly connected to the outer wall on the front side of the bottle seat, the motor is electrically connected to the controller, a convex ring is fixedly connected to the inner wall of the bottle body, the upper part of the bottle body is threadedly connected with a sealing cover, the bottom of the bottle body is rotatably connected with a clamping connection block, a stirring rod is fixedly connected to the top of the clamping connection block, the stirring rod is located inside the bottle body, and the clamping block is clamped with the clamping connection block.

[0007] Furthermore, it also includes a suction pipe, an extrusion bladder, a pipe cap and a one-way valve. The side wall of the bottle body is connected and communicated with a suction pipe, the middle of the suction pipe is connected and communicated with an extrusion bladder, the side of the suction pipe far away from the bottle body is threadedly connected with a pipe cap, and a one-way valve is connected and communicated with the inner wall of the suction pipe.

[0008] Further, it also includes a heating plate and a power supply component. The heating plate is installed on the top of the bottle seat, and the top of the heating plate is in contact with the outer side of the bottom of the bottle body. A power supply component is provided inside the bottle seat. The heating plate is electrically connected to the controller, and the power supply component is electrically connected to the controller.

[0009] Further, it also includes a connecting rod, a hydrophobic cover and a filter membrane. The connecting rod is fixedly connected to the outer wall of the sealing cover. On the side of the connecting rod away from the sealing cover, a hydrophobic cover is fixedly connected. A filter membrane is placed on the inner top of the hydrophobic cover.

[0010] Further, the upper part of the outer wall of the bottle body is provided with a frosted block for firmly attaching a label.

[0011] Further, the controller is provided with a USB interface. The USB interface is connected to a power supply to power on and charge the power supply component.

[0012] Advantages of the present utility model: 1. The present utility model drives the stirring rod to rotate through the motor. The rotation of the stirring rod stirs the cleaning agent and clean water, foams the cleaning agent, and repeatedly scrapes the residues on the inner wall of the bottle body, realizing the deep cleaning of the cell culture bottle, so as to realize the reuse of the cell culture bottle, improve the utilization rate of the cell culture bottle, reduce the culture consumables and costs, and is also beneficial to environmental protection.

[0013] 2. The present utility model heats the bottle body through the heating plate and controls the temperature inside the bottle at a constant temperature through the controller, creating a good living environment for the intestinal cell culture and effectively improving the survival rate of intestinal cells.

[0014] 3. By pressing the extrusion bladder of the present utility model, a pressure difference is formed between the inner and outer walls of the suction pipe, and a certain amount of cleaning agent and clean water are sucked into the bottle body through the suction pipe. Through the single-flow function of the one-way valve, the sucked substances will not flow back. Description of the Drawings

[0015] Figure 1 It is a three-dimensional structure schematic diagram of the present utility model.

[0016] Figure 2 It is a three-dimensional sectional structure schematic diagram of components such as the bottle body, the convex ring and the stirring rod of the present utility model.

[0017] Figure 3 It is a three-dimensional sectional structure schematic diagram of components such as the suction pipe, the extrusion bladder and the pipe cap of the present utility model.

[0018] Figure 4 It is a three-dimensional structure schematic diagram of components such as the motor, the block and the clamping block of the present utility model.

[0019] Figure 5 It is a three-dimensional structure schematic diagram of components such as the sealing cover, the connecting rod and the hydrophobic cover of the present utility model.

[0020] Reference numerals in the drawings: 1 - bottle body, 2 - convex ring, 3 - sealing cover, 301 - connecting rod, 302 - hydrophobic cover, 303 - filter membrane, 4 - bottle base, 5 - motor, 6 - clamping block, 7 - clamping block, 8 - stirring rod, 9 - suction pipe, 10 - extrusion bladder, 11 - pipe cap, 12 - one-way valve, 13 - heating plate, 14 - power supply assembly, 15 - controller. Detailed implementation

[0021] The present utility model will be further described below in conjunction with the drawings and embodiments.

[0022] Embodiment: A self-cleaning automatic stirring bottle for intestinal cell cultivation, as Figures 1 - 5 shown, includes a bottle body 1, a convex ring 2, a sealing cover 3, a bottle base 4, a motor 5, a clamping block 6, a clamping block 7, a stirring rod 8 and a controller 15. The motor 5 is installed at the inner bottom of the bottle base 4 by screws, a clamping block 6 is welded on the output shaft of the motor 5, the bottle body 1 is placed on the top of the bottle base 4, a frosted block is pasted on the upper part of the outer wall of the bottle body 1 by glue for firmly attaching a label, the controller 15 is installed on the front outer wall of the bottle base 4 by screws, the motor 5 is electrically connected to the controller 15, the convex ring 2 is fixedly connected to the upper part of the inner wall of the bottle body 1, the sealing cover 3 is connected to the top of the bottle body 1 in a threaded manner, the clamping block 7 is rotatably connected to the bottom of the bottle body 1, a stirring rod 8 is fixedly connected to the top of the clamping block 7, the stirring rod 8 is located inside the bottle body 1, and the clamping block 6 is clamped with the clamping block 7.

[0023] As Figures 1 - 3 and Figure 5 shown, it further includes a connecting rod 301, a hydrophobic cover 302, a filter membrane 303, a suction pipe 9, an extrusion bladder 10, a pipe cap 11 and a one-way valve 12. The connecting rod 301 is fixedly connected to the middle outer wall of the sealing cover 3, the hydrophobic cover 302 is fixedly connected to the side of the connecting rod 301 away from the sealing cover 3, the filter membrane 303 is placed on the inner top of the hydrophobic cover 302, the suction pipe 9 is connected and communicated with the right side wall of the bottle body 1, the extrusion bladder 10 is connected and communicated with the middle of the suction pipe 9, the pipe cap 11 is connected to the side of the suction pipe 9 away from the bottle body 1 in a threaded manner, and the one-way valve 12 is connected and communicated with the left side of the inner wall of the suction pipe 9.

[0024] First, the staff for intestinal cell cultivation places the bottle horizontally on the table. If short-term airtight adherent cultivation of intestinal cells is required, first rotate the sealing cap 3 clockwise and remove it. At this time, the staff puts the intestinal cells to be cultivated through the bottle mouth, and then rotates the sealing cap 3 counterclockwise to seal the bottle mouth. At this time, the intestinal cells in the bottle body 1 adhere tightly to the convex ring 2 and start to survive, grow, and reproduce, etc. After the intestinal cells are successfully cultivated and reach the cell growth density requirement, the staff first rotates the sealing cap 3 clockwise and removes it, and then adds relevant digestive fluid to digest and collect the intestinal cells. If long-term airtight adherent cultivation of intestinal cells is required, first rotate the hydrophobic cap 302 clockwise and remove it, put the intestinal cells to be cultivated through the bottle mouth, then place the filter membrane 303 on the bottle mouth, and then rotate the hydrophobic cap 302 counterclockwise to seal the bottle mouth. During the cultivation process, when the old culture medium needs to be removed, the bottle body 1 can be directly placed upside down. The filter membrane 303 separates and filters the old culture medium from the intestinal cells. The intestinal cells remain in the bottle, and the old culture medium flows out through the hydrophobic cap 302. Then, new culture medium can be added. After the intestinal cells are cultivated and the intestinal cells are digested and collected, when the cultivation bottle needs to be cleaned and reused, first remove the tube cap 11, and then manually press the extrusion bladder 10. At this time, a pressure difference is formed inside and outside the tube of the suction pipe 9, and a certain amount of cleaning agent and clean water are sucked into the bottle body 1. Under the action of the one-way valve 12, the cleaning agent will not form a backflow. Then, start the motor 5 through the controller 15. The output shaft of the motor 5 rotates to drive the clamping block 6 to rotate, and the clamping block 6 drives the clamping block 7 and the stirring rod 8 to rotate. At this time, the stirring rod 8 stirs the cleaning agent and clean water, makes the cleaning agent foam, and at the same time the stirring rod 8 scrapes the inner wall of the bottle body 1. The residues on the inner wall of the bottle body 1 are scraped. After cleaning for a period of time, after ensuring that the inside of the bottle body 1 is cleaned, rotate the hydrophobic cap 302 clockwise again and remove it. At this time, the bottle mouth is not sealed, and then pour out the substances in the bottle. Repeat the above cleaning steps multiple times to ensure the cleanliness of the bottle.

[0025] As Figure 3 shown, it also includes a heating plate 13 and a power supply assembly 14. The heating plate 13 is installed at the top inside the bottle seat 4 by screws, and the top of the heating plate 13 is in contact with the outer bottom of the bottle body 1. A power supply assembly 14 is provided on the left side of the bottom inside the bottle seat 4. The heating plate 13 is electrically connected to the controller 15, and the power supply assembly 14 is electrically connected to the controller 15. The controller 15 is provided with a USB interface, and the USB interface is connected to the power supply to power on and charge the power supply assembly 14.

[0026] During the cultivation process of intestinal cells, if constant-temperature cell cultivation is required, the staff starts the heating plate 13 through the controller 15. The heating plate 13 heats the bottom of the bottle body 1 and transfers the heat to the inside of the bottle body 1 through heat transfer. At the same time, the temperature of the constant temperature can be adjusted through the controller 15 to ensure the temperature requirement of intestinal cells during the cultivation process. When the power supply component 14 has insufficient power storage, the power supply component 14 can be charged through the USB interface to ensure sufficient electrical energy during the cleaning of the cultivation bottle and the constant-temperature cultivation.

[0027] As described above, it is only the specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention can easily think of changes or substitutions, which should all be covered within the protection scope of the present invention. Therefore, the protection scope of the present invention should be subject to the protection scope of the claims.

Claims

1. A self-cleaning automatic stirring bottle for intestinal cell cultivation, characterized in that, It includes a bottle body (1), a convex ring (2), a sealing cap (3), a bottle base (4), a motor (5), a clamping block (6), a clamping connection block (7), a stirring rod (8) and a controller (15). A motor (5) is fixedly connected inside the bottle base (4). A clamping block (6) is fixedly connected to the output shaft of the motor (5). The bottle body (1) is placed on the top of the bottle base (4). A controller (15) is fixedly connected to the outer wall on the front side of the bottle base (4). The motor (5) is electrically connected to the controller (15). A convex ring (2) is fixedly connected to the inner wall of the bottle body (1). The upper part of the bottle body (1) is threadedly connected with a sealing cap (3). The bottom of the bottle body (1) is rotatably connected with a clamping connection block (7). A stirring rod (8) is fixedly connected to the top of the clamping connection block (7). The stirring rod (8) is located inside the bottle body (1). The clamping block (6) is clamped with the clamping connection block (7).

2. The self-cleaning automatic stirring bottle for intestinal cell cultivation according to claim 1, wherein, It further includes a suction pipe (9), a squeezing bladder (10), a pipe cap (11) and a one-way valve (12). The side wall of the bottle body (1) is connected and communicated with a suction pipe (9). The middle part of the suction pipe (9) is connected and communicated with a squeezing bladder (10). The side of the suction pipe (9) far away from the bottle body (1) is threadedly connected with a pipe cap (11). A one-way valve (12) is connected and communicated with the inner wall of the suction pipe (9).

3. The self-cleaning automatic stirring bottle for intestinal cell cultivation according to claim 2, characterized in that, It further includes a heating plate (13) and a power supply component (14). A heating plate (13) is installed on the top of the bottle base (4), and the top of the heating plate (13) is in contact with the outer side of the bottom of the bottle body (1). A power supply component (14) is arranged inside the bottle base (4). The heating plate (13) is electrically connected to the controller (15). The power supply component (14) is electrically connected to the controller (15).

4. The self-cleaning automatic stirring bottle for intestinal cell cultivation according to claim 3, wherein, It further includes a connecting rod (301), a hydrophobic cap (302) and a filter membrane (303). A connecting rod (301) is fixedly connected to the outer wall of the sealing cap (3). A hydrophobic cap (302) is fixedly connected to the side of the connecting rod (301) far away from the sealing cap (3). A filter membrane (303) is placed on the inner top of the hydrophobic cap (302).

5. The self-cleaning automatic stirring bottle for intestinal cell culture according to claim 4, characterized in that There are abrasive blocks on the upper part of the outer wall of the bottle body (1) for firmly attaching labels.

6. The self-cleaning automatic stirring bottle for intestinal cell culture according to claim 5, characterized in that The controller (15) is provided with a USB interface, and the USB interface is connected to a power supply to charge the power supply component (14).

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