Disposable living cell concentration detection liquid storage bag

Abstract

The utility model discloses a disposable living cell concentration detection liquid storage bag which comprises a liquid storage bag body, a capacitance detection port, a conduit and an optical density detection port, the capacitance detection port is installed on the outer wall of one side of the top of the liquid storage bag body, and the optical density detection port is installed on the outer wall of the other side of the top of the liquid storage bag body. Rubber films are arranged at the tops of the capacitance detection port and the optical density detection port, cross-shaped openings are formed in the rubber films, a sealing sleeve is arranged on the outer wall of the bottom of the liquid storage bag body, a sealing groove is formed in the outer wall of one side of the sealing sleeve, a pressing plate is arranged on one side of the interior of the sealing groove, and a clamping groove is formed in the other side of the sealing groove. One end of each pressing plate is connected with the sealing sleeve through a rotating shaft, the guide pipes are installed at the bottom end of the liquid storage bag body, and the bottom ends of the guide pipes penetrate through the sealing grooves and are provided with sealing covers. According to the utility model, two detection modes of a capacitance method and an optical density method can be satisfied, and the detection accuracy is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of live cell concentration detection, and specifically relates to a disposable live cell concentration detection liquid storage bag. Background Art

[0002] It has broad application prospects in the fields of cell culture, biopharmaceutics, tissue engineering, etc. By real-time monitoring of cell concentration, researchers can better understand the cell growth and metabolism processes, timely discover and solve problems in the cell culture process, optimize cell culture conditions, and improve cell yield and quality.

[0003] The live cell concentration detection liquid storage bag is used to store cell liquid. The liquid storage bag is for single use. The commonly used live cell concentration detection method is the capacitance method. According to the capacitance measurement principle, in a high-frequency electric field, live cells are regarded as tiny capacitors. The charges of these micro-capacitors are measured by electrodes and displayed in the form of dielectric constant (capacitance value per unit area). This measurement is not affected by changes in the culture medium, microcarriers, dead cells or cell debris. However, the detection accuracy of a single detection method is not high, so it is urgently needed to be improved. Summary of the Utility Model

[0004] The purpose of the utility model is to provide a disposable live cell concentration detection liquid storage bag to solve the problems raised in the above background art.

[0005] To achieve the above purpose, the utility model provides the following technical solution: A disposable live cell concentration detection liquid storage bag, including a liquid storage bag body, a capacitance detection port, a catheter and an optical density detection port. The capacitance detection port is installed on one side outer wall at the top of the liquid storage bag body. The optical density detection port is installed on the other side outer wall at the top of the liquid storage bag body. Rubber membranes are provided at the tops of the capacitance detection port and the optical density detection port, and a cross opening is provided inside the rubber membranes. A sealing sleeve is provided on the outer wall at the bottom of the liquid storage bag body, and sealing grooves are provided on the outer walls on one side of the sealing sleeve. A pressing plate is provided on one side inside the sealing groove, and one ends of the pressing plates are connected to the sealing sleeve through rotating shafts. The catheters are all installed at the bottom end of the liquid storage bag body, and the bottom ends of the catheters penetrate through the sealing grooves and are provided with sealing caps.

[0006] Preferably, a handle part is provided at the top end of the liquid storage bag body. The handle part is integrally formed with the liquid storage bag body, and a hanging hole is provided at the central position inside the handle part to facilitate hanging the liquid storage bag body.

[0007] Preferably, sealing membranes are provided inside the capacitance detection port and the optical density detection port below the rubber membranes to facilitate sealing the capacitance detection port and the optical density detection port.

[0008] Preferably, positioning convex edges are provided on both outer side walls of the liquid storage bag body below the sealing sleeve. The positioning convex edges are integrally formed with the liquid storage bag body, facilitating the positioning of the sealing sleeve.

[0009] Preferably, the sealing cover is threadedly connected to the conduit.

[0010] Preferably, a pressing block is fixed on the inner wall of the pressing plate, and the pressing block extends into the sealing groove.

[0011] Preferably, a torsion spring is installed on the outer wall of the rotating shaft to provide elastic force for the rotating shaft.

[0012] Preferably, the sealing grooves correspond to the conduits one by one.

[0013] Compared with the prior art, the beneficial effects of the present utility model are as follows:

[0014] Insert the capacitance probe and the optical density probe into the capacitance detection port and the optical density detection port successively. The capacitance probe and the optical density probe pass through the cross opening inside the rubber film and pierce the sealing film and then extend into the cell liquid inside the liquid storage bag body to perform the detection of the viable cell concentration. This design can meet the two detection methods of the capacitance method and the optical density method, thereby improving the detection accuracy. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 is the front view structural schematic diagram of the present utility model;

[0016] Figure 2 is of the present utility model Figure 1 the enlarged structural schematic diagram at A in;

[0017] Figure 3 is the enlarged side view structural schematic diagram of the pressing block of the present utility model;

[0018] Figure 4 is the side view structural schematic diagram of the present utility model;

[0019] Figure 5 is of the present utility model Figure 4 the enlarged structural schematic diagram at B in.

[0020] In the figure: 1. Liquid storage bag body; 2. Handheld part; 3. Hanging hole; 4. Capacitance detection port; 5. Rubber film; 6. Cross opening; 7. Sealing sleeve; 8. Positioning convex edge; 9. Conduit; 10. Sealing cover; 11. Sealing groove; 12. Pressing plate; 13. Rotating shaft; 14. Torsion spring; 15. Pressing block; 16. Optical density detection port; 17. Sealing film. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0021] To make the objectives, technical solutions, and advantages of the embodiments of the present utility model clearer, the technical solutions in the embodiments of the present utility model will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present utility model. Therefore, the following detailed description of the embodiments of the present utility model provided in the drawings is not intended to limit the scope of the present utility model to be protected, but merely represents the selected embodiments of the present utility model. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present utility model without creative efforts fall within the scope of protection of the present utility model.

[0022] The technical solutions in the embodiments of the present utility model will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present utility model. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present utility model without creative efforts fall within the scope of protection of the present utility model.

[0023] Please refer to Figures 1-5 , an embodiment provided by the present utility model: a disposable live cell concentration detection liquid storage bag, including a liquid storage bag body 1, a capacitance detection port 4, a catheter 9, and an optical density detection port 16. The capacitance detection port 4 is installed on one outer wall of the top of the liquid storage bag body 1, and the optical density detection port 16 is installed on the other outer wall of the top of the liquid storage bag body 1. Rubber membranes 5 are provided at the tops of the capacitance detection port 4 and the optical density detection port 16, and a cross opening 6 is provided inside the rubber membrane 5;

[0024] Specifically, a capacitance probe and an optical density probe are inserted into the capacitance detection port 4 and the optical density detection port 16 successively. The capacitance probe and the optical density probe pass through the cross opening 6 inside the rubber membrane 5, pierce the sealing film 17, and extend into the cell liquid inside the liquid storage bag body 1 to perform live cell concentration detection. This design can meet two detection methods, namely the capacitance method and the optical density method, thereby improving the detection accuracy;

[0025] A sealing sleeve 7 is provided on the outer wall of the bottom of the liquid storage bag body 1, and sealing grooves 11 are provided on the outer wall of one side of the sealing sleeve 7. A pressing plate 12 is provided on one side inside the sealing groove 11, and one end of the pressing plate 12 is connected to the sealing sleeve 7 through a rotating shaft 13;

[0026] The catheters 9 are all installed at the bottom end of the liquid storage bag body 1, and the bottom ends of the catheters 9 penetrate through the sealing groove 11 and are provided with sealing caps 10;

[0027] Specifically, since one end of the catheter 9 is stuck in the sealing groove 11 on the outer wall of the sealing sleeve 7, the pressing plate 12 tightly clamps the catheter 9 through a pressing block 15 under the elastic action of a torsion spring 14, playing a role in stopping water, thereby achieving an anti-leakage effect;

[0028] When it is necessary to drain the cell liquid, first pull the pressing plate 12 to make it rotate around the rotating shaft 13. The pressing plate 12 drives the pressing block 15 to move away from the surface of the conduit 9, take out the conduit 9 from the sealing groove 11, and then unscrew the sealing cover 10, and the cell liquid inside the liquid storage bag body 1 can be drained through the conduit 9;

[0029] A handle part 2 is arranged at the top end of the liquid storage bag body 1. The handle part 2 is integrally formed with the liquid storage bag body 1, and a hanging hole 3 is arranged at the central position inside the handle part 2;

[0030] Specifically, the liquid storage bag body 1 can be hung and placed through the hanging hole 3 inside the handle part 2;

[0031] Sealing films 17 are arranged inside the capacitance detection port 4 and the optical density detection port 16 below the rubber film 5;

[0032] Positioning convex edges 8 are arranged on both outer side walls of the liquid storage bag body 1 below the sealing sleeve 7. The positioning convex edges 8 are integrally formed with the liquid storage bag body 1;

[0033] Specifically, lifting the sealing sleeve 7 can detach it from the surface of the liquid storage bag body 1. The sealing sleeve 7 can be reused, and the liquid storage bag body 1 is for one-time use;

[0034] The sealing cover 10 is in threaded connection with the conduit 9;

[0035] A pressing block 15 is fixed on the inner wall of the pressing plate 12, and the pressing block 15 extends into the sealing groove 11;

[0036] A torsion spring 14 is installed on the outer wall of the rotating shaft 13;

[0037] The sealing grooves 11 correspond to the conduits 9 one by one.

[0038] When the embodiment of the present application is in use: First, a capacitance detection port 4 and an optical density detection port 16 are respectively installed on the outer walls of the top of the liquid storage bag body 1. The capacitance detection port 4 is used for the detection of a capacitance probe, and the optical density detection port 16 is used for the detection of an optical density probe. Specifically, the capacitance probe and the optical density probe are successively inserted into the capacitance detection port 4 and the optical density detection port 16. The capacitance probe and the optical density probe pass through the cross opening 6 inside the rubber film 5 and pierce the sealing film 17 and then extend into the cell liquid inside the liquid storage bag body 1 for the detection of the viable cell concentration. This design can meet the two detection methods of the capacitance method and the optical density method, thereby improving the detection accuracy. Then, since one end of the catheter 9 is stuck in the sealing groove 11 on the outer wall of the sealing sleeve 7, the pressing plate 12 clamps the catheter 9 through the pressing block 15 under the elastic action of the torsion spring 14, which plays a role in preventing water leakage, thus achieving the effect of preventing leakage. Then, the liquid storage bag body 1 can be hung and placed through the hanging hole 3 inside the handle portion 2. When it is necessary to drain the cell liquid, first pull the pressing plate 12 to rotate it around the rotating shaft 13. The pressing plate 12 drives the pressing block 15 to move away from the surface of the catheter 9, and the catheter 9 is taken out of the sealing groove 11. Then, the sealing cover 10 is unscrewed, and the cell liquid inside the liquid storage bag body 1 can be drained through the catheter 9. Moreover, the sealing sleeve 7 can be removed from the surface of the liquid storage bag body 1 by lifting it up. The sealing sleeve 7 can be reused, and the liquid storage bag body 1 is for single use.

[0039] Obviously, the above-described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without making creative efforts shall fall within the protection scope of the present invention.

Claims

1. A disposable liquid storage bag for detecting the concentration of living cells, characterized in that, It includes a liquid storage bag body (1), a capacitance detection port (4), a catheter (9), and an optical density detection port (16). The capacitance detection port (4) is installed on the outer wall of one side of the top of the liquid storage bag body (1), and the optical density detection port (16) is installed on the outer wall of the other side of the top of the liquid storage bag body (1). Rubber membranes (5) are provided at the tops of the capacitance detection port (4) and the optical density detection port (16), and a cross opening (6) is provided inside the rubber membrane (5). A sealing sleeve (7) is provided on the outer wall of the bottom of the liquid storage bag body (1), and sealing grooves (11) are provided on the outer wall of one side of the sealing sleeve (7). A pressing plate (12) is provided on one side inside the sealing groove (11), and one end of the pressing plate (12) is connected to the sealing sleeve (7) through a rotating shaft (13). The catheters (9) are all installed at the bottom end of the liquid storage bag body (1), and the bottom ends of the catheters (9) penetrate through the sealing grooves (11) and are provided with sealing caps (10).

2. The disposable liquid storage bag for detecting the concentration of living cells according to claim 1, wherein: A carrying handle (2) is provided at the top end of the liquid storage bag body (1). The carrying handle (2) is integrally formed with the liquid storage bag body (1), and a hanging hole (3) is provided at the central position inside the carrying handle (2).

3. The disposable liquid storage bag for detecting the concentration of living cells according to claim 1, characterized in that: A sealing film (17) is provided inside the capacitance detection port (4) and the optical density detection port (16) below the rubber membrane (5).

4. A disposable live cell concentration detection reservoir bag according to claim 1, characterized in that: Positioning convex edges (8) are provided on the outer walls of both sides of the liquid storage bag body (1) below the sealing sleeve (7). The positioning convex edges (8) are integrally formed with the liquid storage bag body (1).

5. A disposable storage bag for detecting the concentration of live cells according to claim 1, characterized in that: The sealing cap (10) is threadedly connected to the catheter (9).

6. A disposable reservoir bag for detecting the concentration of live cells according to claim 1, characterized in that: A pressing block (15) is fixed to the inner wall of the pressing plate (12), and the pressing block (15) extends into the sealing groove (11).

7. A disposable liquid storage bag for detecting live cell concentration according to claim 1, characterized in that: A torsion spring (14) is installed on the outer wall of the rotating shaft (13).

8. A disposable reservoir bag for detecting the concentration of live cells according to claim 1, characterized in that: The sealing grooves (11) correspond to the catheters (9) one by one.

Images