Mouse caudal vein blood sampling fixing device
By designing a mouse tail vein blood retrieval fixation device, using a light-shielding protective cover and a flexible restraint belt combined with a food inducer, the problems of difficulty in the tail vein blood retrieval and stress death in mice were solved, and efficient and low-cost blood retrieval and fixation effect was achieved.
Patent Information
- Application Number
- CN202421152936.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-24
- Publication Date
- 2025-07-08
- Estimated Expiration
- 2034-05-24
AI Technical Summary
The blood retrieval operation of the tail vein of mice is difficult, the success rate is low, and existing fixed equipment is prone to stress death, which is high in experimental costs.
A mouse tail vein blood extraction fixation device is designed, including a base and a reversible light-shielding protective cover, a flexible binding belt and a food attractant placement slot, and a tail fixation ring and an injection needle fixation ring to create a dark environment. It is gentlely fixed by a flexible binding belt, soothes the mice with food attractants and reduces stress response.
It improves the survival rate of blood retrieval in the tail vein of mice, reduces operation difficulty, reduces stress response, is low in material cost, is suitable for large-scale production, is easy to operate, and is reusable.
Smart Images
Figure CN223068639U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of living biological experimental instruments, and in particular relates to a mouse tail vein blood sampling and fixing device. Background Art
[0002] At present, mice (typically mice) are a common experimental animal in medical research, and are also commonly used animals in experiments in medical physiology, pharmacology, pathology, pathophysiology and other disciplines. Blood collection from the tail vein of mice is one of the common and necessary operations in the fields of pharmacology, toxicology, genetics, tumors, immunology and other biological experiments and basic research in medical experiments. However, since the tail vein of mice is extremely thin, and mice have high resistance when restrained, and resist violently during the injection process, it is quite difficult to draw blood from the tail vein of mice during the experimental operation, and the success rate is low. Operators are easily bitten and scratched, and mice are also prone to stress death. Without any auxiliary equipment, experimental operators must complete no less than one hundred injection blood collection training to achieve a high success rate.
[0003] Taking pharmacokinetic experiments as an example, during the observation and study of mice, it is necessary to repeatedly draw blood from the tail vein. Therefore, it is necessary to ensure the survival rate and survival time of the experimental mice as much as possible, so as to support multiple tail vein blood draws and continuous research over a long period of time. Although some existing experimental mouse fixation equipment can achieve strong fixation of mice, it often puts too much pressure on the mice and easily leads to stress. The death of mice due to stress loses the research value, which is detrimental to subsequent experiments, and the experimental cost increases accordingly. Based on the above-mentioned difficulties in venous blood drawing from mice, it is necessary to propose a new mouse tail vein blood drawing and fixation device that is easy to operate and less irritating to mice. Utility Model Content
[0004] The utility model aims to solve the deficiencies of the prior art and provide a mouse tail vein blood sampling and fixing device which is easy to operate and has little irritation to mice.
[0005] In order to achieve the above object, the technical solution of the utility model is implemented as follows:
[0006] The utility model provides a blood collection fixing device for mouse tail vein, which comprises a base and a light-shielding protective cover rotatably connected above the base. The base comprises a main fixing seat facing the light-shielding protective cover and a tail fixing seat extending from the main fixing seat. A flexible restraint strap and a bait placement groove are arranged on the main fixing seat. One end of the flexible restraint strap is fixedly connected to the main fixing seat, and the other end is detachably connected to the main fixing seat to adjust the tightness of restraining the mouse's torso. A tail outlet hole corresponding to the mouse tail is arranged on the light-shielding protective cover. A tail fixing ring corresponding to the mouse tail and an injection needle fixing ring for fixing an injection needle are arranged on the tail fixing seat. The injection needle fixing ring is rotatably connected to the tail fixing seat.
[0007] In one embodiment, the other end of the flexible restraint strap is detachably connected to the main fixing seat through a buckle. The buckle comprises a fixing rod fixedly connected to the main fixing seat, a buckle frame and a buckle needle rotatably connected to the fixing rod. A plurality of buckle holes are arranged on the flexible restraint strap opposite to the buckle needle.
[0008] In one embodiment, a magic tape round hair surface is arranged on the inner side of the other end of the flexible restraint strap, and a magic tape prickly hair surface is arranged on the surface of the main fixing seat opposite to the magic tape round hair surface.
[0009] In one embodiment, a sticky silica gel layer is bonded to the bottom of the base. The water-proof and pollution-proof sticky silica gel layer is beneficial to the stable placement of the whole device, and the sticky silica gel layer can be adsorbed on an experimental ultra-clean table, a stainless steel table top or a plastic experimental table.
[0010] In one embodiment, a warm-keeping fluff is bonded to the inner side of the flexible restraint strap. The flexible restraint strap can be prepared from one of soft materials such as non-woven fabric, artificial leather, cowhide, etc. With the warm-keeping fluff bonded to the inner side, it is beneficial to better alleviate the stimulated reaction of the mouse during tail vein blood collection.
[0011] In one embodiment, the base and the light-shielding protective cover are hinged through a hinge.
[0012] In one embodiment, a breathable observation window is arranged on the light-shielding protective cover for the experimental operator to observe and for the mouse to breathe.
[0013] In one embodiment, a limiting groove is recessed in the bottom edge of the main fixing seat opposite to the light-shielding protective cover. When the light-shielding protective cover covers the main fixing seat, the bottom edge is placed in the limiting groove to prevent the light-shielding protective cover from shifting.
[0014] In one embodiment, a handle is arranged on the top of the light-shielding protective cover.
[0015] In one embodiment, two coaxial tail fixing rings are arranged in sequence on the tail fixing seat, and one injection needle fixing ring is arranged on each side of the two tail fixing rings.
[0016] Compared with the prior art, the beneficial effects of the utility model are as follows: a dark environment is created for the mouse by using a light-shielding protective cover, the trunk of the mouse is gently fixed by a flexible restraint belt, there is no need to fix it manually in real time, it is not easy to loosen, and it is combined with the attractant in the attractant placement groove for soothing, avoiding the mouse from colliding with the fixing device when panicked, greatly reducing the stress reaction of the mouse when it is restrained, effectively improving the survival rate of the mouse after tail vein blood collection. The tail fixing ring and the injection needle fixing ring provided on the tail fixing seat facilitate adjusting the injection needle to an appropriate angle for collecting blood from the tail vein of the mouse, are easy to operate, reduce the difficulty of tail vein blood collection, have low material cost, simple manufacturing process, are suitable for large-scale production and preparation, can be sterilized by high-pressure steam, and can be reused. Brief Description of the Drawings
[0017] Figure 1 is a schematic structural diagram of an embodiment of the utility model;
[0018] Figure 2 is a schematic structural diagram of another embodiment of the utility model;
[0019] Figure 3 is Figure 2 a schematic structural diagram of the needle buckle in
[0020] Figure 4 is an operation schematic diagram of collecting blood from the tail vein of a mouse by using the mouse tail vein blood collection fixing device of the utility model.
[0021] Description of the reference numerals: 1 base, 11 main fixing seat, 13 tail fixing seat, 15 limit groove, 17 hinge installation position, 2 light-shielding protective cover, 21 tail outlet hole, 23 breathable observation window, 25 handle, 3 flexible restraint belt, 31 buckle hole, 4 attractant placement groove, 5 tail fixing ring, 6 injection needle fixing ring, 7 needle buckle, 71 fixing rod, 73 buckle frame, 75 buckle needle, 8 mouse tail, 9 injection needle. Detailed Description of the Embodiments
[0022] Such as Figure 1As shown in the figure, this embodiment provides a mouse tail vein blood sampling and fixing device, which includes a base 1 and a light-shielding protective cover 2 hinged above the base 1 by a hinge. An air-permeable observation window 23 is provided on the light-shielding protective cover 2, and a handle 25 is provided at the top. A sticky silica gel layer is bonded to the bottom of the base 1. The base 1 and the light-shielding protective cover 2 can be formed by injection molding with materials such as stainless steel, acrylic material, and polyethylene plastic. A limiting groove 15 is recessed on the main fixing seat 11 of the base 1 opposite to the bottom edge of the light-shielding protective cover 2. When the light-shielding protective cover 2 is covered on the main fixing seat 11, the bottom edge is placed in the limiting groove 15. A tail outlet hole 21 with a diameter of about 1 cm is provided on the light-shielding protective cover 2 corresponding to the mouse tail.
[0023] The base 1 includes a main fixing seat 11 opposite to the light-shielding protective cover 2 for limiting the mouse trunk and a tail fixing seat 13 extending from the main fixing seat 11 for mouse tail fixing and blood sampling. A flexible restraint belt 3 and a food attractant placement groove 4 are provided on the main fixing seat 11. A warm fleece is bonded to the inner side of the flexible restraint belt 3. One end of the flexible restraint belt 3 is fixedly connected to the main fixing seat 11, and the other end is detachably connected to the main fixing seat 11 to adjust the tightness of restraining the mouse trunk. Specifically, a magic tape round hair surface is provided on the inner side of the other end of the flexible restraint belt 3, and a magic tape prickly hair surface is provided on the surface of the main fixing seat 11 opposite to the magic tape round hair surface.
[0024] As Figure 2 and Figure 3 shown, in other embodiments, the other end of the flexible restraint belt 3 can also be detachably connected to the main fixing seat 11 through a buckle 7. The buckle 7 includes a fixed rod 71 fixedly connected to the main fixing seat 11, a buckle frame 73 rotatably connected to the fixed rod 71, and a buckle pin 75. A plurality of buckle holes 31 are provided on the flexible restraint belt 3 opposite to the buckle pin 75 for cooperating with the buckle 7 to adjust the tightness of restraint. The fixed rod 71 of the buckle 7 is fixedly connected to the main fixing seat 11. The width of the fixed rod 71 and the fixed end of the flexible restraint belt 3 is pre-determined to conform to the general body width of the mouse. The buckle frame 73 and the buckle pin 75 are respectively rotatably connected to the fixed rod 71. When the movable end of the flexible restraint belt 3 needs to be fixed, first rotate the buckle frame 73 to make the movable end of the flexible restraint belt 3 pass through the buckle frame 73, then rotate the buckle pin 75 to adjust the tightness of the flexible restraint belt 3 fitting with the mouse trunk, and pass the buckle pin 75 through the buckle hole at the corresponding position on the movable end of the flexible restraint belt 3 to make the flexible restraint belt 3 basically fit with the mouse trunk, realizing gentle fixation.
[0025] The distance from the front end of the flexible restraint band 3 on the main fixing base 11 to the hinge mounting position 17 is limited to a distance that is insufficient for the mouse to escape from the flexible restraint band 3, and the hinge installed at the hinge mounting position 17 can rule out the possibility of the mouse pushing open the light-shielding protective cover 2 and escaping. After the light-shielding protective cover 2 is closed, a relatively dark environment is created for the mouse, and mice are generally quieter in a relatively dark environment. The movable end of the flexible restraint band 3 is detachably connected to the main fixing base 11 by means of Velcro or a snap button, which is not only convenient for operation but also enables appropriate adjustment according to the volume of the mouse when the flexible restraint band 3 is used to fix the mouse's torso, so that while restraining the mouse, it will not compress the mouse and cause a stress response. Together with the attractant placed in the attractant placement groove 4 in front of the mouse, the mouse can be made even quieter. Moreover, a warm fleece is adhered to the inner side of the flexible restraint band 3, which can better soothe the mouse and improve the survival rate of the mouse after tail vein blood collection.
[0026] In this embodiment, two coaxial tail fixing rings 5 are arranged in sequence on the tail fixing base 13 corresponding to the mouse's tail. At the same time, an injection needle fixing ring 6 for fixing the injection needle is provided. The injection needle fixing ring 6 is rotatably connected to the tail fixing base 13, and an injection needle fixing ring 6 is provided on each side of the two tail fixing rings 5. As Figure 4 shown, when an experimenter performs tail vein blood collection, first pass the needle part of the injection needle through the injection needle fixing ring 6 for preliminary fixation of the needle, and then rotate the injection needle fixing ring 6 to adjust to a suitable injection angle. The injection needle fixing ring 6 plays a role in adjusting and stabilizing the injection angle of the needle, further reducing the operation difficulty of vein blood collection. The material for preparing the tail fixing ring 5 is one of nylon, polypropylene plastic, and non-woven fabric. The inner diameter of the front tail fixing ring 5 is about 0.6 cm, and the inner diameter of the rear tail fixing ring 5 is about 0.3 cm.
[0027] During the blood collection process, place the base 1 on the experimental operation plane. The temperature of the experimental operation plane should not exceed 60°C. Open the light-shielding protective cover 2, put the attractant in the attractant placement groove 4, then put the mouse in, fix the mouse body in the flexible restraint band 3, fix the mouse tail through the two tail fixing rings 5, cover the light-shielding protective cover 2 again, and then insert the syringe into the rotatable injection needle fixing ring 6 and adjust to a suitable angle to collect blood from the mouse's tail vein.
[0028] The blood collection and fixation device for mouse tail vein of the present utility model creates a dark environment for the mouse by using a light-shielding protective cover, gently fixes the mouse's torso with a flexible restraint band, and coordinates with the attractant in the attractant placement groove for soothing. It does not require manual real-time fixation, is not easy to loosen, and greatly reduces the stress response of the mouse when being restrained, effectively improving the survival rate of the mouse after tail vein blood collection. The tail fixing ring and injection needle fixing ring provided on the tail fixing seat facilitate adjusting the injection needle to an appropriate angle for blood collection from the mouse tail vein, are easy to operate, reduce the difficulty of tail vein blood collection, have low material costs, simple manufacturing processes, are suitable for large-scale production and preparation, can be sterilized by high-pressure steam, and can be reused.
[0029] Those of ordinary skill in the art should understand that the discussion of any embodiment above is only exemplary and is not intended to imply that the scope of protection of the present application is limited to these examples; under the concept of the present application, the technical features in the above embodiments or different embodiments can also be combined, the steps can be implemented in any order, and there are many other variations in different aspects of one or more embodiments of the present application as described above, which are not provided in detail for the sake of brevity.
[0030] One or more embodiments of the present application are intended to cover all such substitutions, modifications, and variations that fall within the broad scope of the present application. Therefore, any omission, modification, equivalent substitution, improvement, etc. made within the spirit and principle of one or more embodiments of the present application shall be included within the scope of protection of the present application.
Claims
1. A mouse tail vein blood collection and fixation device, characterized in that, It includes a base (1) and a light-shielding protective cover (2) which is reversibly connected above the base (1). The base (1) includes a main fixing seat (11) facing the light-shielding protective cover (2) and a tail fixing seat (13) extending from the main fixing seat (11). A flexible restraint strap (3) and a luring agent placement groove (4) recessed are provided on the main fixing seat (11). One end of the flexible restraint strap (3) is fixedly connected to the main fixing seat (11), and the other end is detachably connected to the main fixing seat (11) to adjust the tightness of restraining the mouse's torso. A tail outlet hole (21) corresponding to the mouse's tail is provided on the light-shielding protective cover (2). A tail fixing ring (5) and an injection needle fixing ring (6) for fixing an injection needle corresponding to the mouse's tail are provided on the tail fixing seat (13). The injection needle fixing ring (6) is rotatably connected to the tail fixing seat (13).
2. The mouse tail vein blood sampling and fixation device according to claim 1, characterized in that, The other end of the flexible restraint strap (3) is detachably connected to the main fixing seat (11) through a snap button (7). The snap button (7) includes a fixing rod (71) fixedly connected to the main fixing seat (11), a buckle frame (73) and a buckle pin (75) rotatably connected to the fixing rod (71). A plurality of buckle holes (31) are provided on the flexible restraint strap (3) opposite to the buckle pin (75).
3. The mouse tail vein blood collection and fixation device according to claim 1, wherein, A magic tape round hair surface is provided on the inner side of the other end of the flexible restraint strap (3), and a magic tape prickly hair surface is provided on the surface of the main fixing seat (11) facing the magic tape round hair surface.
4. The mouse tail vein blood sampling and fixing device according to any one of claims 1-3, characterized in that, A sticky silica gel layer is bonded to the bottom of the base (1).
5. The mouse tail vein blood collection and fixation device according to any one of claims 1-3, characterized in that, Warmth-keeping fluff is bonded to the inner side of the flexible restraint strap (3).
6. The mouse tail vein blood sampling and fixation device according to any one of claims 1-3, characterized in that, The base (1) and the light-shielding protective cover (2) are hinged through a hinge.
7. The mouse tail vein blood collection and fixation device according to any one of claims 1-3, characterized in that A breathable observation window (23) is provided on the light-shielding protective cover (2).
8. The mouse tail vein blood sampling and fixing device according to any one of claims 1-3, characterized in that, A limiting groove (15) is recessed on the main fixing seat (11) opposite to the bottom edge of the light-shielding protective cover (2). When the light-shielding protective cover (2) covers the main fixing seat (11), the bottom edge is placed in the limiting groove (15).
9. The mouse tail vein blood sampling and fixation device according to any one of claims 1-3, characterized in that, A handle (25) is provided on the top of the light-shielding protective cover (2).
10. The mouse tail vein blood sampling and fixation device according to any one of claims 1-3, characterized in that, Two coaxially arranged tail fixing rings (5) are provided on the tail fixing seat (13) in sequence, and an injection needle fixing ring (6) is provided on each side of the two tail fixing rings (5).