Five-day biochemical oxygen demand detection device
By using dissolved oxygen in situ detection membrane and image acquisition components in the five-day biochemical oxygen demand detection device, the sealing and detection accuracy problems of sample are solved, and efficient dissolved oxygen concentration detection and sample culture are achieved.
Patent Information
- Application Number
- CN202422126826.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-30
- Publication Date
- 2025-07-08
- Estimated Expiration
- 2034-08-30
AI Technical Summary
In the prior art, during the five-day biochemical oxygen demand detection, the sealing properties of the sample are difficult to maintain, resulting in low culture efficiency, and the dissolved oxygen detection method destroys the culture environment, affecting the detection accuracy and efficiency.
Using a detection device including a sample vial, a sealing cap and a dissolved oxygen in-situ detection membrane, the dissolved oxygen in-situ detection membrane is attached to the side wall of the sample vial, combined with an image acquisition component and a light source, the in-situ detection of dissolved oxygen is achieved, the sealing of the sample is ensured, and the detection results are determined whether to dilute the sample and continue to culture.
Without destroying the sealing properties of the sample, the accurate detection of dissolved oxygen concentration is achieved, the sample culture efficiency is improved, the need for sample dilution and reculture is avoided, and the detection accuracy and efficiency are improved.
Smart Images
Figure CN223078191U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of water quality detection, in particular to a device for detecting five-day biochemical oxygen demand. Background Art
[0002] Under the determination conditions of BOD (sufficient oxygen, no agitation), it generally takes 20 days for general organic matter to basically complete the oxidation decomposition process in the first stage (99% of the process is completed); that is to say, it takes 20 days to determine the biochemical oxygen demand in the first stage, which is difficult to achieve in actual work. Therefore, a standard time is stipulated. Generally, 5 days is used as the standard time for determining BOD, so it is called five-day biochemical oxygen demand, denoted as BOD5. BOD5 is about 70% of BOD20.
[0003] When detecting five-day biochemical oxygen demand (BOD5), the sample needs to be cultured for 5 days under completely sealed conditions. If the dissolved oxygen concentration in the sample is lower than 2mg / L after 5 days, the sample needs to be diluted and cultured again. In the prior art, when detecting the five-day biochemical oxygen demand of the sample after 5 days of culture, generally the iodometric method or the electrochemical probe method is used to test the dissolved oxygen in the cultured sample. Although this method can complete the test, it destroys the culture environment and pollutes the sample; thus, when the sample needs to be diluted and cultured again, since the seal of the original sample is damaged, a new sample needs to be replaced and cultured again, which makes the culture efficiency of the sample low. Therefore, how to ensure the tightness of the sample during the dissolved oxygen concentration detection and how to improve the culture efficiency of the sample are technical problems to be solved urgently. Summary of the Utility Model
[0004] In view of this, the utility model provides a device for detecting five-day biochemical oxygen demand to solve one or more technical problems existing in the prior art.
[0005] According to one aspect of the utility model, the utility model provides a device for detecting five-day biochemical oxygen demand, and the detection device includes:
[0006] A sample bottle, having a bottle mouth and a cavity, and the cavity is used for containing the sample to be detected for five-day biochemical oxygen demand;
[0007] A sealing cap, used for covering the bottle mouth;
[0008] An in-situ dissolved oxygen detection membrane, located in the cavity of the sample bottle, and the in-situ dissolved oxygen detection membrane is attached to the side wall of the sample bottle.
[0009] In some embodiments of the present utility model, the detection device includes a light-blocking layer, the light-blocking layer is located inside the cavity of the sample bottle, and the light-blocking layer is located on a side of the in-situ dissolved oxygen detection membrane away from the side wall of the sample bottle.
[0010] In some embodiments of the present utility model, there is a gap between the light-blocking layer and the in-situ dissolved oxygen detection membrane.
[0011] In some embodiments of the present utility model, the gap between the light-blocking layer and the in-situ dissolved oxygen detection membrane is 2 mm to 8 mm.
[0012] In some embodiments of the present utility model, the detection device includes an image acquisition component, the image acquisition component is located on one side of the sample bottle, and the image acquisition component is used to acquire an image of the in-situ dissolved oxygen detection membrane.
[0013] In some embodiments of the present utility model, the sample bottle is a square bottle, and the top edge and the bottom edge of the in-situ dissolved oxygen detection membrane are respectively flush with the top edge and the bottom edge of the side wall of the sample bottle.
[0014] In some embodiments of the present utility model, the side wall of the sample bottle is a transparent side wall.
[0015] In some embodiments of the present utility model, the detection device further includes a light source, the light source is located on the same side of the sample bottle as the image acquisition component, and the light source is located above the image acquisition component.
[0016] In some embodiments of the present utility model, the distance range between the light source and the center of the in-situ dissolved oxygen detection membrane is 60 cm to 70 cm, and the distance range between the image acquisition component and the center of the in-situ dissolved oxygen detection membrane is 20 cm to 30 cm.
[0017] In some embodiments of the present utility model, the in-situ dissolved oxygen detection membrane includes a PET layer and a gel layer, and the gel layer is located on the surface of the PET layer.
[0018] In the above embodiments of the present utility model, the five-day biochemical oxygen demand detection device includes a sample bottle, a sealing cover, and an in-situ dissolved oxygen detection membrane. The in-situ dissolved oxygen detection membrane is located inside the cavity of the sample bottle, and the in-situ dissolved oxygen detection membrane is attached to the side wall of the sample bottle. The five-day biochemical oxygen demand detection device can determine the dissolved oxygen concentration of the sample in the sealed state based on the in-situ dissolved oxygen detection membrane inside it, thereby ensuring the tightness of the sample during the dissolved oxygen concentration detection based on in-situ detection. And when it is detected that the dissolved oxygen content of the sample in the sample bottle is not less than 2 mg / L, the sample continues to be used for cultivation. If it is less than 2 mg / L, the sample is diluted additionally for cultivation. This device improves the cultivation efficiency of the sample.
[0019] Additional advantages, objects, and features of the present utility model will be partially described below, and will become partially apparent to those of ordinary skill in the art after studying the following text, or can be learned from the practice of the present utility model. The objects and other advantages of the present utility model can be achieved and obtained by the structures specifically pointed out in the written description, its claims, and the drawings.
[0020] Those skilled in the art will understand that the objects and advantages that can be achieved by the present utility model are not limited to the above specifically described, and the above and other objects that the present utility model can achieve will be more clearly understood according to the following detailed description. BRIEF DESCRIPTION OF THE DRAWINGS
[0021] The drawings described herein are used to provide a further understanding of the present utility model, form a part of this application, and do not limit the present utility model. The components in the drawings are not drawn to scale, but are only for showing the principles of the present utility model. For the convenience of showing and describing some parts of the present utility model, the corresponding parts in the drawings may be enlarged, that is, they may become larger relative to other components in the exemplary device actually manufactured according to the present utility model. In the drawings:
[0022] Figure 1 is a schematic structural diagram of a five-day biochemical oxygen demand detection device according to an embodiment of the present utility model.
[0023] Figure 2 is a schematic structural diagram of a five-day biochemical oxygen demand detection device according to another embodiment of the present utility model.
[0024] Figure 3 is a schematic position diagram of the in-situ dissolved oxygen detection membrane according to an embodiment of the present utility model.
[0025] Reference Numerals:
[0026] Sample bottle 100, Sealing cover 200, In-situ dissolved oxygen detection membrane 300, Light-blocking layer 400, Image acquisition component 500, Light source 600 Detailed implementation mode
[0027] To make the objectives, technical solutions, and advantages of the embodiments of the present utility model clearer and more understandable, the following further elaborates on the embodiments of the present utility model in conjunction with the accompanying drawings. Herein, the illustrative embodiments of the present utility model and their descriptions are used to explain the present utility model, but do not limit the present utility model.
[0028] Herein, it should be noted that in order to avoid obscuring the present utility model due to unnecessary details, only the structures and / or processing steps closely related to the solution according to the present utility model are shown in the drawings, while other details less related to the present utility model are omitted.
[0029] It should be emphasized that the terms "comprising / including / having" when used herein refer to the presence of features, elements, steps, or components, but do not exclude the presence or addition of one or more other features, elements, steps, or components.
[0030] Herein, it also needs to be explained that the orientation terms such as "left end" and "right end" in the content of this specification are relative to the position directions shown in the drawings; if there is no special explanation, the term "connection" in this article can not only refer to direct connection, but also represent indirect connection with an intermediate object. Direct connection means that two components are connected without the aid of an intermediate component, and indirect connection means that two components are connected with the aid of other components.
[0031] In the following, embodiments of the present utility model will be described with reference to the accompanying drawings. In the drawings, the same reference numerals represent the same or similar components.
[0032] Figure 1 is a structural schematic diagram of a five-day biochemical oxygen demand detection device according to an embodiment of the present utility model. As Figure 1 shown, the five-day biochemical oxygen demand detection device at least includes a sample bottle 100, a sealing cap 200, and a dissolved oxygen in-situ detection membrane 300.
[0033] The sample bottle 100 has a bottle mouth and a cavity, and the cavity is used to hold the sample to be detected for five-day biochemical oxygen demand; the sealing cap 200 is used to seal the bottle mouth; the dissolved oxygen in-situ detection membrane 300 is located in the cavity of the sample bottle 100, and the dissolved oxygen in-situ detection membrane 300 is attached to the side wall of the sample bottle 100.
[0034] In this embodiment, the five-day biochemical oxygen demand detection device realizes in-situ detection of dissolved oxygen based on the in-situ dissolved oxygen detection membrane 300, and determines whether the dissolved oxygen in the cultured sample is lower than 2 mg / L without destroying the sealed culture state. If it is not lower than 2 mg / L, the sample is used for continuous culture. If it is lower than 2 mg / L, the sample is diluted additionally for culture. Therefore, detecting the dissolved oxygen concentration of the sample based on the five-day biochemical oxygen demand detection device not only ensures the tightness of the sample, but also improves the culture efficiency of the sample.
[0035] Furthermore, the detection device includes an image acquisition component 500. The image acquisition component 500 is located on one side of the sample bottle 100, and the image acquisition component is used to acquire an image of the in-situ dissolved oxygen detection membrane 300. Specifically, the image acquisition component can be a camera, which is used to take an image of the in-situ dissolved oxygen detection membrane 300, so as to analyze the concentration of dissolved oxygen in the sample based on the fluorescence intensity in the acquired image. It can be understood that the image acquisition component in this embodiment can be specifically fixedly connected to the sample bottle 100 during use, or fixed on a bracket beside the sample bottle 100. Figure 1 It can be seen that the camera is specifically located on the side of the sample bottle 100 close to the in-situ dissolved oxygen detection membrane 300.
[0036] In another embodiment, the detection device further includes a light source 600. The light source 600 is located on the same side of the sample bottle 100 as the image acquisition component 500, and the light source 600 is located above the image acquisition component 500. The light source 600 is used to emit light to the in-situ dissolved oxygen detection membrane 300, so that the light intensity of the in-situ dissolved oxygen detection membrane 300 changes under the action of the sample based on the received light emitted by the light source 600, and then the dissolved oxygen content in the sample is determined based on the change in light intensity. Similar to the image acquisition component, the light source 600 can also be fixed at the corresponding position through a bracket, or when the sample bottle 100 is fixed, the light source 600 can also be fixedly connected to the sample bottle 100.
[0037] In the above embodiment, an in-situ dissolved oxygen (DO) detection membrane is attached to the side wall of the sample bottle 100, with the fluorescent indicator facing inward and in contact with the sample in the sample bottle 100. Therefore, during the test, the wavelength of the light source 600 is set to 450 nm. That is, under the excitation of the excitation light source at 450 nm, an image of the in-situ dissolved oxygen detection membrane 300 is collected by a camera (with a filter placed), and further through the image decoding method in the prior art, the concentration of dissolved oxygen in the sample is obtained. It can be understood that how to analyze the dissolved oxygen content of the sample based on the image belongs to the prior art and is not the inventive point of this application, so it will not be elaborated in this application.
[0038] In some embodiments of the present utility model, the detection device includes a light-blocking layer 400. The light-blocking layer 400 is located inside the cavity of the sample bottle 100, and the light-blocking layer 400 is located on the side of the in-situ dissolved oxygen detection membrane 300 away from the side wall of the sample bottle 100. In this embodiment, the light-blocking layer 400 and the side wall of the sample bottle 100 are respectively located on the left and right sides of the in-situ dissolved oxygen detection membrane 300. Thus, when the light source 600 emits light towards the in-situ dissolved oxygen detection membrane 300, the light-blocking layer 400 prevents light from spilling out, thereby ensuring that the in-situ dissolved oxygen detection membrane 300 better receives the light emitted by the light source 600, and further ensuring the accuracy of the dissolved oxygen detection result. Optionally, the light-blocking layer 400 can be implemented by a black light-blocking plate; it can be understood that using the black light-blocking plate as the light-blocking layer 400 in this embodiment is only a preferred example and does not constitute a limitation on the light-blocking layer 400.
[0039] Furthermore, in order to enable the in-situ dissolved oxygen detection membrane 300 to effectively contact the sample inside the sample bottle 100, a gap is provided between the light-blocking layer 400 and the in-situ dissolved oxygen detection membrane 300. This gap can be filled with the sample, thereby ensuring the effective and accurate detection of the dissolved oxygen content of the sample by the in-situ dissolved oxygen detection membrane 300. Exemplarily, the gap between the light-blocking layer 400 and the in-situ dissolved oxygen detection membrane 300 is 2 mm to 8 mm; optionally, the gap between the light-blocking layer 400 and the in-situ dissolved oxygen detection membrane 300 is specifically set to 5 mm. At this time, the light-blocking layer 400 can play a better light-blocking role, and the 5-mm gap can also enable the in-situ dissolved oxygen detection membrane 300 to contact an adequate amount of sample.
[0040] In one embodiment, the sample bottle 100 is a square bottle. At this time, the in-situ dissolved oxygen detection membrane 300 is located on one of the side walls of the square bottle, and the top edge and the bottom edge of the in-situ dissolved oxygen detection membrane 300 are respectively flush with the top edge and the bottom edge of the side wall of the sample bottle 100. In this embodiment, the in-situ dissolved oxygen detection membrane 300 is the same height as the side wall of the sample bottle 100. Therefore, the in-situ dissolved oxygen detection membrane 300 extends from the top edge of the sample bottle 100 towards the bottom edge and penetrates in the height direction of the side wall of the sample bottle 100. This structure further improves the detection effectiveness of the in-situ dissolved oxygen detection membrane 300.
[0041] It can be understood that when the sample bottle 100 is a square bottle, the in-situ dissolved oxygen detection membrane 300 can be located on any one of the side walls of the sample bottle 100; in addition, in one embodiment, the in-situ dissolved oxygen detection membrane 300 can also be provided on two or more side walls of the square bottle at the same time. Moreover, setting the shape of the sample bottle 100 as a square is only for illustration. In other embodiments, the sample bottle 100 can also be in other shapes other than square, as long as its sealing performance is ensured.
[0042] In addition, to facilitate the detection of the light intensity on the in-situ dissolved oxygen detection membrane 300, the side wall of the sample bottle 100 is provided as a transparent side wall, which will not affect the light intensity on the in-situ dissolved oxygen detection membrane 300, thereby ensuring the accuracy of the detected dissolved oxygen concentration result. In a specific embodiment, the material of the side wall of the sample bottle 100 is quartz glass. It can be understood that using quartz glass as the material of the sample bottle 100 is only an example. In other embodiments, the sample bottle 100 can also be made of other types of transparent materials. As Figure 3 shown, the total thickness range of the side wall of the sample bottle, the in-situ dissolved oxygen detection membrane, and the light-blocking layer in this embodiment can be between 2 cm and 3 cm.
[0043] As Figure 2 shown in the embodiment, the light source 600 is located directly in front of the in-situ dissolved oxygen detection membrane 300. In this embodiment, the in-situ dissolved oxygen detection membrane 300 is located on the right side wall of the square bottle, and the light source 600 is correspondingly located directly on the right side of the square bottle. Specifically, the position of the light source 600 relative to the sample bottle 100 is fixed, that is, the light source 600 can be fixed on its independent bracket or directly fixedly connected to the sample bottle 100; similarly, the image acquisition component 500 is also located on the right side of the square bottle, and the image acquisition component 500 is located above the light source 600. In this embodiment, the position of the image acquisition component 500 relative to the sample bottle 100 is fixed, that is, the image acquisition component 500 can be fixed on its independent bracket or directly fixedly connected to the sample bottle 100.
[0044] Optionally, the distance range between the light source 600 and the center of the in-situ dissolved oxygen detection membrane 300 is 60 cm to 70 cm, and the distance range between the image acquisition component 500 and the center of the in-situ dissolved oxygen detection membrane 300 is 20 cm to 30 cm. Setting a certain distance between the light source 600 and the in-situ dissolved oxygen detection membrane 300, and between the image acquisition component 500 and the in-situ dissolved oxygen detection membrane 300 is to ensure that the in-situ dissolved oxygen detection membrane 300 evenly receives the light emitted by the light source 600 and to ensure the integrity of the captured image of the in-situ dissolved oxygen detection membrane. Optionally, the distance between the light source 600 and the center of the in-situ dissolved oxygen detection membrane 300 is 23 cm, and the distance between the image acquisition component 500 and the center of the in-situ dissolved oxygen detection membrane 300 is 66 cm.
[0045] In the above embodiment, an in-situ detection film capable of measuring dissolved oxygen (DO) is adhered to the inner side of the side wall of the sample bottle 100, and the in-situ detection film has a specific fluorescent indicator, which can emit fluorescence under the excitation of light of a specific wavelength. When the fluorescent indicator on the in-situ detection film contacts the sample, the intensity of the fluorescence decreases as the concentration of dissolved oxygen in the sample increases, so that the embodiment can determine the concentration of dissolved oxygen in the sample by measuring the change in the fluorescence intensity on the dissolved oxygen in-situ detection film 300. In the five-day biochemical oxygen demand detection device, the process of obtaining the fluorescent image does not need to destroy the sealed culture state of the sample bottle 100, so that the in-situ test of dissolved oxygen can be realized, and whether the sample needs to be diluted can also be determined according to the change in the concentration of dissolved oxygen.
[0046] Exemplarily, the dissolved oxygen in-situ detection membrane 300 includes a PET layer and a gel layer, and the gel layer is located on the surface of the PET layer. Specifically, the dissolved oxygen in-situ detection membrane 300 uses the PET layer as a carrier, and when making the gel layer, toluene and ethanol solvents are mixed in a ratio of 4:1. Specifically, 50mL of a mixed solution of toluene and ethanol dissolves 7.5mg of tridichlororuthenium and 1g of ethyl cellulose, stirs into a transparent gel, and then coats it on the surface of the PET layer through a coating machine. In one embodiment, the thickness of the gel layer may be 10μm, and the monitoring range of the dissolved oxygen in-situ detection membrane 300 of this embodiment is 0 to 15mg / L.
[0047] Through the above embodiments, it can be found that the device of the present application can determine the dissolved oxygen concentration of the sample in a sealed state based on the dissolved oxygen in-situ detection membrane inside it, thereby ensuring the sealing of the sample during the dissolved oxygen concentration detection process based on the in-situ detection; and when it is detected that the dissolved oxygen content of the sample in the sample bottle is not less than 2 mg / L, the sample continues to be used for culture, if it is less than 2 mg / L, the sample is further diluted for culture; the device improves the culture efficiency of the sample.
[0048] In the present invention, features described and / or illustrated for one embodiment may be used in the same manner or in a similar manner in one or more other embodiments, and / or combined with features of other embodiments or replace features of other embodiments.
[0049] The above-listed embodiments show and describe the basic principles and main features of the utility model, but the utility model is not limited to the above-mentioned embodiments. Any modifications, equivalent changes and modifications made to the utility model by those skilled in the art without making any creative work should fall within the scope of protection of the technical solution of the utility model.
Claims
1. A device for detecting five-day biochemical oxygen demand, characterized in that, The detection device includes: A sample bottle having a bottle mouth and a cavity, and the cavity is used to hold a sample to be detected for five-day biochemical oxygen demand; A sealing cap for covering the bottle mouth; An in-situ dissolved oxygen detection membrane located in the cavity of the sample bottle, and the in-situ dissolved oxygen detection membrane is attached to the side wall of the sample bottle.
2. The five-day biochemical oxygen demand detection device according to claim 1, wherein, The detection device includes a light-blocking layer, the light-blocking layer is located in the cavity of the sample bottle, and the light-blocking layer is located on the side of the in-situ dissolved oxygen detection membrane away from the side wall of the sample bottle.
3. The five-day biochemical oxygen demand detection device according to claim 2, characterized in that, There is a gap between the light-blocking layer and the in-situ dissolved oxygen detection membrane.
4. The five-day biochemical oxygen demand detection device according to claim 3, characterized in that, The gap between the light-blocking layer and the in-situ dissolved oxygen detection membrane is 2 mm to 8 mm.
5. The biochemical oxygen demand detection device according to claim 1, wherein The detection device includes an image acquisition component, the image acquisition component is located on one side of the sample bottle, and the image acquisition component is used to acquire an image of the in-situ dissolved oxygen detection membrane.
6. The five-day biochemical oxygen demand detection device according to claim 1, characterized in that, The sample bottle is a square bottle, and the top edge and the bottom edge of the in-situ dissolved oxygen detection membrane are respectively flush with the top edge and the bottom edge of the side wall of the sample bottle.
7. The five-day biochemical oxygen demand detection device according to claim 6, characterized in that, The side wall of the sample bottle is a transparent side wall.
8. The five-day biochemical oxygen demand detection device according to claim 5, characterized in that, The detection device further includes a light source, the light source is located on the same side of the sample bottle as the image acquisition component, and the light source is located above the image acquisition component.
9. The five-day biochemical oxygen demand detection device according to claim 8, characterized in that, The distance between the light source and the center of the in-situ dissolved oxygen detection membrane ranges from 60 cm to 70 cm, and the distance between the image acquisition component and the center of the in-situ dissolved oxygen detection membrane ranges from 20 cm to 30 cm.
10. The five-day biochemical oxygen demand detection device according to claim 1, characterized in that, The in-situ dissolved oxygen detection membrane includes a PET layer and a gel layer, and the gel layer is located on the surface of the PET layer.