TAL (tachypleus amebocyte lysate) sample adding device

Abstract

The utility model discloses a tachypleus amebocyte lysate sample adding device which comprises a base, a temperature control module arranged in the base, a plurality of storage grooves formed in the top end of the base, V-shaped bottom ends of the storage grooves, sample adding grooves formed in the storage grooves, covers arranged at the top ends of the sample adding grooves, and a plurality of sample adding holes formed in the top ends of the covers. The problems that waste is easily caused due to the fact that an existing sample adding device cannot conduct sampling completely, and an existing sampling device cannot be directly applied to automation equipment are solved.

Description

Technical Field

[0001] The utility model relates to the technical field of medical equipment, in particular to a horseshoe crab reagent sampling device. Background Art

[0002] Endotoxin detection is crucial in the quality control of invasive products such as injections and medical devices. For many years, horseshoe crab reagents extracted from the blood cells of Chinese horseshoe crabs have been regarded as the "gold standard" for endotoxin detection due to their highly specific reaction with endotoxins. However, the production of traditional horseshoe crab reagents severely relies on the capture and blood collection of wild horseshoe crabs.

[0003] The dynamic chromogenic method and the dynamic turbidimetry method use enzyme-linked immunosorbent assay (ELISA) plates for detection. Generally, a multi-channel pipette is used for sampling to reduce the impact of sampling on the detection time. When using the existing reagent troughs, the volume of horseshoe crab reagent that cannot be aspirated is relatively large, resulting in reagent waste. At the same time, with the emergence of the current automated dilution and sampling endotoxin determination system, since the existing reagent troughs cannot be fixed to automated equipment, the internal reagent needs to be transferred to an ELISA plate for subsequent operations. In automated equipment, the reagent needs to be prepared in advance, and the reagent will volatilize. Excessive operation steps will accelerate the volatilization of the reagent. Summary of the Utility Model

[0004] The utility model aims to provide a horseshoe crab reagent sampling device to solve the problems that the existing sampling device cannot sample completely, easily causing waste, and the existing sampling device cannot be directly applied to automated equipment.

[0005] To achieve the above object, the utility model provides the following technical solution: A horseshoe crab reagent sampling device includes a base. A temperature control module is arranged inside the base. A plurality of placement grooves are arranged at the top of the base. The bottom end of the placement groove is in a "V" shape. A sampling groove with an open top is arranged inside the placement groove. A lid is arranged at the top of the sampling groove. A plurality of sampling holes are opened at the top of the lid.

[0006] Preferably, the temperature control module includes a thermoelectric cooler, a temperature sensor, a storage battery, and a controller that are electrically connected. The thermoelectric cooler and the temperature sensor are placed in contact with the placement groove and the sampling groove.

[0007] Preferably, a micro-scale is arranged on the inner wall of the sampling groove.

[0008] Preferably, the sampling groove is made of transparent polypropylene or PTFE material.

[0009] Preferably, the length and width of the base are 120 mm × 80 mm.

[0010] Principle and Beneficial Effects of this Technical Solution:

[0011] (1) The bottom of the sample adding groove is designed to be more sharp V-shaped, so that the reagent can be concentrated at the bottom, and the sample can be absorbed as completely as possible when the sample is taken with the discharge gun, reducing waste.

[0012] (2) Micro-scale is engraved on the inner wall of the sample adding groove to facilitate observation of the remaining amount.

[0013] (3) The sample loading tank is made of transparent chemically inert materials such as polypropylene or PTFE to avoid reaction with the horseshoe crab reagent. At the same time, the material has a certain degree of transparency, which facilitates observation of the liquid surface.

[0014] (4) The lid on the top of the sample loading slot can reduce reagent evaporation, and the small holes on the lid facilitate sample loading.

[0015] (5) The base size is the same as that of an ELISA plate and can be adapted to automated dilution equipment.

[0016] (6) The temperature control module of the base can ensure that the horseshoe crab reagent is kept at a low temperature before adding the sample. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 A schematic diagram of the structure of a horseshoe crab reagent adding device provided in an embodiment of the utility model;

[0018] Figure 2 A cross-sectional view of a horseshoe crab reagent adding device provided in an embodiment of the utility model;

[0019] In the figure: 1. Base; 2. Storage slot; 3. Sample adding slot; 4. Cover; 5. Sample adding hole. DETAILED DESCRIPTION

[0020] The present invention is further described in detail below with reference to the accompanying drawings and implementation modes:

[0021] like Figure 1 A horseshoe crab reagent loading device shown includes a base, the length and width of the base are 120mm×80mm, which is the size of an ELISA plate, and is used to adapt to automated dilution equipment so that the device can be used directly without transferring the reagent to the ELISA plate. A temperature control module is provided inside the base. The horseshoe crab reagent requires a low temperature before loading. The temperature control module includes an electrically connected cooling sheet, a temperature sensor, a battery and a controller. The cooling sheet and the temperature sensor are placed in contact with the storage slot and the loading slot. The temperature sensor detects the temperature. When the temperature is high, the cooling sheet can be turned on to cool the reagent.

[0022] There are several storage grooves provided at the top of the base. The sample addition groove is made of transparent polypropylene or PTFE. The bottom end of the storage groove is in a "V" shape, which can ensure that the reagent is extracted as much as possible without waste when a multi-channel pipette is used to extract the reagent. A sample addition groove with an open top is provided inside the storage groove, and micro-scales are provided on the inner wall of the sample addition groove. A lid is provided at the top of the sample addition groove, and several sample addition holes are opened at the top of the lid, through which reagents can be directly added or extracted into the interior. The rest of the part is covered by the lid, which can reduce volatilization. At the same time, the sample addition groove is a disposable item and is discarded after use, while the base can be used multiple times. The sample addition groove is used for endotoxin detection, and its own endotoxin content should be less than 0.001 EU / ml.

[0023] The specific implementation process is as follows:

[0024] In the case of applying methods such as the kinetic chromogenic method and the kinetic turbidimetric method that require transferring the reagent inside the device, place the required sample addition groove in the storage groove on the base. After adding the limulus reagent into the sample addition groove, cover it with the lid. When extraction is needed, open the lid and use a multi-channel pipette to extract it; when using an automated device, place the sample addition groove in the storage groove on the base. After adding the limulus reagent into the sample addition groove, cover it with the lid, and then place it together with the base into the automated device, and the device will automatically perform the subsequent operations.

[0025] The above are only the embodiments of the present utility model. Specific technical solutions or common knowledge such as characteristics known to the public are not described in detail here. For those skilled in the art, without departing from the technical solution of the present utility model, several deformations and improvements can be made, which should also be regarded as the protection scope of the present utility model, and these will not affect the implementation effect of the present utility model and the practicality of the patent. The protection scope required by this application should be based on the content of its claims, and the specific implementation manners described in the specification can be used to interpret the content of the claims.

Claims

1. A limulus reagent sampling device, characterized in that: It includes a base, a temperature control module is arranged inside the base, several placing grooves are arranged at the top end of the base, the bottom end of the placing groove is in a "V" shape, a sample adding groove with an open top is arranged in the placing groove, a lid is arranged at the top end of the sample adding groove, and several sample adding holes are opened at the top end of the lid.

2. The limulus reagent sampling device according to claim 1, wherein: The temperature control module includes a refrigeration sheet, a temperature sensor, a storage battery and a controller which are electrically connected, and the refrigeration sheet and the temperature sensor are placed in contact with the placing groove and the sample adding groove.

3. The limulus reagent sampling device according to claim 1, characterized in that: Microscopic scales are arranged on the inner wall of the sample adding groove.

4. A limulus reagent sampling device according to claim 1, wherein: The sample adding groove is made of transparent polypropylene or PTFE material.

5. The limulus reagent sampling device according to claim 1, characterized in that: The length and width of the base are 120mm×80mm.

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