High-pressure-resistant empty chromatography column capable of being repeatedly installed and used

Abstract

The utility model relates to a high pressure resistant chromatography empty column capable of being repeatedly installed and used, which comprises a column body, a sealing cover is movably installed at the top of the column body, a filter plate is arranged in the column body, a dismounting mechanism is arranged in the column body, the dismounting mechanism comprises a pull rod, the pull rod is arranged in the column body, and the sealing cover is arranged on the top of the column body. A first clamping ring is arranged on the front side of the pull rod, the pull rod is sleeved with a sliding sleeve, and a second clamping ring is arranged on the front side of the sliding sleeve. According to the high-pressure-resistant empty chromatography column capable of being repeatedly installed and used, firstly, clamping blocks of filter plates are clamped into clamping grooves, the filter plates are preliminarily fixed, then a second clamping base is closed and locked through a fixing screw, a second filter plate is installed in a second clamping ring in the same method, then a user pulls a sliding sleeve up and down, and then the second clamping ring can be driven to move up and down; and when the second clamping ring moves to a proper position, the fastening bolt is rotated to stably fix the second clamping ring, and finally, the sealing cover is closed.

Description

Technical Field

[0001] The utility model relates to the technical field of chromatography empty columns, and specifically relates to a highly pressure-resistant chromatography empty column that can be repeatedly installed and used. Background Technique

[0002] The chromatography empty column is designed specifically for antigen-antibody, recombinant protein purification, protein desalting, etc. It can be filled with chromatography media with separation mechanisms such as immune affinity, ion exchange, gel filtration, and reversed phase according to needs, and is a powerful tool for purifying antibodies, proteins, and other molecules.

[0003] For example, a kind of affinity chromatography empty column with the Chinese patent number CN212016816U. The outer wall of this utility model is provided with a capacity scale, which is convenient for staff to accurately use the corresponding volume of solution, thus improving the experimental effect and efficiency. The upper cover is snap-connected to the column tube for easy disassembly and assembly. However, when this device is used, the filtering device and the column tube are an integrated structure, which leads to inconvenient cleaning of the column tube after long-term use, thus being not conducive to the repeated use of the chromatography empty column. Therefore, a highly pressure-resistant chromatography empty column that can be repeatedly installed and used is proposed to solve the above-mentioned problems. Content of the Utility Model

[0004] Aiming at the deficiencies of the prior art, the utility model provides a highly pressure-resistant chromatography empty column that can be repeatedly installed and used, which has the advantage of being convenient for the repeated use of the chromatography empty column, and solves the problem that when this device is used, the filtering device and the column tube are an integrated structure, which leads to inconvenient cleaning of the column tube after long-term use, thus being not conducive to the repeated use of the chromatography empty column.

[0005] To achieve the above purpose, the utility model provides the following technical solution: A highly pressure-resistant chromatography empty column that can be repeatedly installed and used, including a column body, a sealing cover is movably installed at the top of the column body, a filtering plate is arranged inside the column body, and a disassembly mechanism is arranged inside the column body;

[0006] The disassembly mechanism includes a pull rod. A pull rod is arranged inside the column body. A first clamping ring is arranged on the front side of the pull rod. A sliding sleeve is sleeved outside the pull rod. A second clamping ring is arranged on the front side of the sliding sleeve. A positioning hole is opened on the rear side of the pull rod, and a fastening bolt is arranged inside the positioning hole.

[0007] Further, clamping blocks are installed on the left sides of both of the two filtering plates. Claw slots adapted to the clamping blocks are opened inside both the first clamping ring and the second clamping ring. The pull rod penetrates through the sealing cover and extends into the inside of the column body. A lead-out pipe is connected to the bottom of the column body.

[0008] Further, the number of the filtering plates is two. Both of the two filtering plates are circular. The two filtering plates are respectively located inside the first clamping ring and the second clamping ring.

[0009] Further, the first snap ring includes a first clamping seat and a second clamping seat, and the bottoms of the first clamping seat and the second clamping seat are hinged to each other.

[0010] Further, fixing blocks are fixedly installed on opposite sides of the first clamping seat and the second clamping seat, a fixing screw is arranged outside the first clamping seat, and the two fixing blocks are threadedly connected by the fixing screw.

[0011] Further, anti-slip blocks are fixedly installed on the outsides of the first snap ring and the second snap ring, the number of the positioning holes is not less than five, the fastening bolt passes through the positioning hole and is threadedly connected with the sliding sleeve, and a scale is installed on the outside of the column body.

[0012] Compared with the prior art, the technical solution of the present application has the following beneficial effects:

[0013] For the high-pressure chromatography empty column that can be repeatedly installed and used, by providing a sealing cover, the top of the column body is sealed, and by providing a filter plate, the materials entering the column body are filtered and purified. By providing a disassembly mechanism, the disassembly mechanism includes a pull rod, a first snap ring, a sliding sleeve, a second snap ring, a positioning hole and a fastening bolt. The structures of the disassembly mechanism cooperate with each other to facilitate the removal of the devices inside the column body, so as to facilitate the cleaning of the column body and the replacement of the filter plate. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 is a three-dimensional schematic diagram of the present utility model;

[0015] Figure 2 is a sectional schematic diagram of the present utility model;

[0016] Figure 3 is a partial side view schematic diagram of the present utility model;

[0017] Figure 4 is a partial top view schematic diagram of the present utility model.

[0018] In the figure: 1 column body, 2 sealing cover, 3 filter plate, 4 disassembly mechanism, 401 pull rod, 402 first snap ring, 4021 first clamping seat, 4022 second clamping seat, 403 sliding sleeve, 404 second snap ring, 405 positioning hole, 406 fastening bolt, 5 anti-slip block, 6 scale. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0019] The technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model.

[0020] Please refer to Figures 1-4 , a highly pressure-resistant chromatography empty column that can be repeatedly installed and used, including a column body 1, a sealing cover 2 is movably installed at the top of the column body 1, a filter plate 3 is arranged inside the column body 1, and a disassembly mechanism 4 is arranged inside the column body 1;

[0021] The disassembly mechanism 4 includes a pull rod 401, a pull rod 401 is arranged inside the column body 1, a first clamping ring 402 is arranged on the front side of the pull rod 401, a sliding sleeve 403 is sleeved outside the pull rod 401, a second clamping ring 404 is arranged on the front side of the sliding sleeve 403, a positioning hole 405 is opened on the rear side of the pull rod 401, and a fastening bolt 406 is arranged inside the positioning hole 405.

[0022] In Figure 1 and Figure 2 , clamping blocks are installed on the left sides of the two filter plates 3, clamping grooves adapted to the clamping blocks are opened inside the first clamping ring 402 and the second clamping ring 404, the pull rod 401 penetrates through the sealing cover 2 and extends into the column body 1, and a lead-out pipe is connected to the bottom of the column body 1.

[0023] In Figure 1 and Figure 2 , the number of the filter plates 3 is two. By arranging the filter plates 3, the materials entering the column body 1 are filtered and purified. The two filter plates 3 are both circular, and the two filter plates 3 are respectively located inside the first clamping ring 402 and the second clamping ring 404.

[0024] In Figure 2 and Figure 4 , by arranging the disassembly mechanism 4, the disassembly mechanism 4 includes a pull rod 401, a first clamping ring 402, a sliding sleeve 403, a second clamping ring 404, a positioning hole 405 and a fastening bolt 406. The structures of the disassembly mechanism 4 cooperate with each other to facilitate the removal of the devices inside the column body 1, thereby facilitating the cleaning of the column body 1 and the replacement of the filter plate 3. The first clamping ring 402 includes a first clamping seat and a second clamping seat, and the bottom of the first clamping seat 4021 is hinged to the bottom of the second clamping seat 4022.

[0025] In Figure 3 and Figure 4 , fixing blocks are fixedly installed on the opposite sides of the first clamping seat 4021 and the second clamping seat 4022, a fixing screw is arranged outside the first clamping seat 4021, and the two fixing blocks are connected by screwing the fixing screw.

[0026] In Figure 3 and Figure 4In it, anti-slip blocks 5 are fixedly installed on the outsides of the first snap ring 402 and the second snap ring 404. The number of positioning holes 405 is not less than five. The fastening bolts 406 pass through the positioning holes 405 and are threadedly connected to the sliding sleeve 403. A scale 6 is installed on the outside of the column body 1. By setting the sliding sleeve 403, the user can move the second snap ring 404 up and down by toggling the sliding sleeve 403 up and down, so as to achieve the purpose of adjusting the distance between the first snap ring 402 and the second snap ring 404, and thus adapt to the purification requirements of different types of materials.

[0027] In summary, for this reusable and highly pressure-resistant chromatographic empty column, by setting the pull rod 401, it is convenient to position the first snap ring 402. By setting the sliding sleeve 403, the user can move the second snap ring 404 up and down by toggling the sliding sleeve 403 up and down, so as to achieve the purpose of adjusting the distance between the first snap ring 402 and the second snap ring 404, and thus adapt to the purification requirements of different types of materials. By setting the positioning holes 405, when the second snap ring 404 moves to a suitable position, rotate the fastening bolt 406 to fix the second snap ring 404 stably with the fastening bolt 406.

[0028] Moreover, during use, first, snap the clamping block of the filter plate 3 into the inside of the card slot to initially fix the filter plate 3. Then, close the second card seat 4022 and lock it with the fixing screw. Install the second filter plate 3 in the second snap ring 404 in the same way. Then, the user can move the second snap ring 404 up and down by toggling the sliding sleeve 403 up and down. When the second snap ring 404 moves to a suitable position, rotate the fastening bolt 406 to fix the second snap ring 404 stably with the fastening bolt 406. Finally, close the sealing cover 2. When disassembly is required, open the sealing cover 2 and pull up the pull rod 401 to withdraw the first snap ring 402 and the second snap ring 404 from the column body 1, solving the problem that the filtering device and the column tube are of an integrated structure during the use of the device, which results in inconvenient cleaning of the column tube after long-term use and is thus not conducive to the repeated use of the chromatographic empty column.

[0029] For the specific model specifications of each device in this article, they need to be selected and determined according to the actual specifications of the device, etc. The specific selection and calculation method adopts the existing technology in this field, so it will not be elaborated in detail.

[0030] It should be noted that, in this text, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprising", "including" or any other variation thereof are intended to cover non-exclusive inclusion, such that a process, method, article or device comprising a series of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article or device. Without further limitation, an element defined by the phrase "comprising a..." does not exclude the presence of additional identical elements in the process, method, article or device comprising the said element.

[0031] Although embodiments of the present invention have been shown and described, those of ordinary skill in the art will appreciate that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A highly pressure-resistant chromatography empty column that can be repeatedly installed and used, comprising a column body (1), characterized in that: A sealing cover (2) is movably installed at the top of the cylinder body (1). A filter plate (3) is arranged inside the cylinder body (1), and a disassembly mechanism (4) is arranged inside the cylinder body (1). The disassembly mechanism (4) includes a pull rod (401). The pull rod (401) is arranged inside the cylinder body (1). A first snap ring (402) is arranged on the front side of the pull rod (401). A sliding sleeve (403) is sleeved outside the pull rod (401). A second snap ring (404) is arranged on the front side of the sliding sleeve (403). A positioning hole (405) is formed on the rear side of the pull rod (401), and a fastening bolt (406) is arranged inside the positioning hole (405).

2. A highly pressure-resistant chromatography empty column that can be repeatedly installed and used, as described in claim 1, wherein: Blocks are installed on the left sides of the two filter plates (3). Claw slots adapted to the blocks are formed inside the first snap ring (402) and the second snap ring (404). The pull rod (401) penetrates through the sealing cover (2) and extends into the cylinder body (1). A lead-out pipe is connected to the bottom of the cylinder body (1).

3. A high-pressure-resistant chromatography empty column that can be repeatedly installed and used according to claim 1, characterized in that: The number of the filter plates (3) is two. The two filter plates (3) are both circular. The two filter plates (3) are respectively located inside the first snap ring (402) and the second snap ring (404).

4. A high-pressure-resistant chromatography empty column that can be repeatedly installed and used according to claim 1, characterized in that: The first snap ring (402) includes a first seat and a second seat. The bottoms of the first seat (4021) and the second seat (4022) are hinged to each other.

5. A high-pressure-resistant chromatography empty column that can be repeatedly installed and used according to claim 4, characterized in that: Fixing blocks are fixedly installed on the opposite sides of the first seat (4021) and the second seat (4022). A fixing screw is arranged outside the first seat (4021). The two fixing blocks are connected by the fixing screw in a threaded manner.

6. A highly pressure-resistant chromatography empty column that can be repeatedly installed and used according to claim 1, characterized in that: Anti-slip blocks (5) are fixedly installed on the outsides of the first snap ring (402) and the second snap ring (404). The number of the positioning holes (405) is not less than five. The fastening bolt (406) penetrates through the positioning hole (405) and is in threaded connection with the sliding sleeve (403). A scale (6) is installed outside the cylinder body (1).

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