Packaging device for microspore culture

Abstract

The utility model discloses a packaging device for microspore culture, which comprises an incubator body, a sealing assembly, a reset containing assembly, a partition plate and a top limiting assembly, the partition plate is fixedly arranged at the bottom of the center of the inner cavity of the incubator body, the reset containing assembly is arranged at the center of the inner cavity of the incubator body, and the top limiting assembly is arranged at the bottom of the inner cavity of the incubator body. The top limiting assemblies are arranged on the two sides of the top of the reset containing assembly, the sealing assembly is arranged on the left side of the incubator body, the sealing assembly comprises a sealing door, a control panel, an L-shaped mounting support, an air cylinder, a positioning frame and a mounting base, and the reset containing assembly comprises a containing frame, a clamping groove, a sliding block, a first spring and a sliding rail. The packaging device for microspore culture has the advantages of being convenient to take and place and good in fixing effect, and solves the problems that a culture dish is inconvenient to fix when an incubator in the prior art is used, and meanwhile, the culture dish is troublesome to take and place.

Description

Technical Field

[0001] The utility model relates to the technical field of encapsulated culture of rapeseed microspores, and particularly relates to an encapsulation device for microspore culture. Background Art

[0002] Rapeseed microspores refer to male gametophytes from the release of tetrads formed by meiosis of pollen mother cells until before the formation of mature pollen grains. Under normal circumstances, the tetrad forms four uninucleate microspores, the uninucleate microspores divide to form binucleate microspores, the binucleate microspores continue to develop to form trinucleate microspores, and finally develop into mature pollen grains. During in vitro culture, the selected late uninucleate microspores do not divide into binucleate but divide into two equal daughter cells, and the two daughter cells continue to divide equally into four cells, and so on until they become multicellular clusters. The cell clusters continue to divide and develop into embryoids with different shapes, and the embryoids further develop into regenerated plants or first develop into adventitious buds and callus, and then develop into plants.

[0003] Generally, when culturing rapeseed microspores, the culture needs to be placed in a culture dish and encapsulated with a suspension for culture. During culture, the temperature of the culture needs to be controlled, and the culture dish needs to be transferred to an incubator for cultivation. However, the existing incubators are inconvenient to fix the culture dish during use, and it is more troublesome to take and place. Therefore, there is an urgent need for an encapsulation device for microspore culture to overcome the above defects. Summary of the Utility Model

[0004] The purpose of the utility model is to provide an encapsulation device for microspore culture, which has the advantages of convenient taking and placing and good fixing effect, so as to solve the problems raised in the above background art.

[0005] To achieve the above object, the present utility model provides the following technical solutions: A packaging device for microspore culture, comprising a culture box body, a sealing component, a reset holding component, a partition board and a top limiting component. The partition board is fixedly installed at the bottom of the center of the inner cavity of the culture box body. The reset holding component is arranged at the center of the inner cavity of the culture box body. The top limiting components are both arranged on both sides of the top of the reset holding component. The sealing component is arranged on the left side of the culture box body. The sealing component includes a sealing door, a control panel, an L-shaped mounting bracket, a cylinder, a positioning bracket and a mounting seat. The reset holding component includes a receiving rack, a card slot, a slider, a first spring and a slide rail. The top limiting component includes a positioning plate, a sliding sleeve, a second spring, a sliding rod and a fixing rod. The mounting seats are fixedly installed at the top and bottom of the left side of the front surface of the culture box body. The sealing door is rotatably connected inside the mounting seat through a rotating shaft. The L-shaped mounting bracket is fixedly installed on the left side of the culture box body. The positioning bracket is fixedly installed at one end of the left side of the sealing door. The cylinder is movably connected to the left side of the positioning bracket through a positioning pin. The slide rails are fixedly installed on both sides of the inner cavity of the culture box body. The slider slides inside the slide rail. The receiving rack is fixedly installed on the side where the sliders are relatively close to each other.

[0006] Further, the left side of the cylinder is rotatably connected to the inner cavity of the L-shaped mounting bracket through a positioning pin. The first springs are fixedly installed on both sides of the back surface of the receiving rack.

[0007] Further, the back surface of the first spring is fixedly connected to the inner cavity of the culture box body. The card slots are both opened on both sides inside the receiving rack.

[0008] Further, the sliding sleeves are fixedly installed on both sides of the top of the center of the inner cavity of the culture box body. The sliding rod slides inside the sliding sleeve.

[0009] Further, the positioning plate is fixedly installed at the bottom of the sliding rod. The second springs are fixedly installed at the bottom of the sliding sleeves.

[0010] Further, the bottom of the second spring is fixedly connected to the top of the positioning plate. The fixing rods are both fixedly installed on both sides of the bottom of the positioning plate.

[0011] Further, the control panel is fixedly installed on the left side of the sealing door. A handle is fixedly installed on the top of the positioning plate.

[0012] In summary, due to the adoption of the above technology, the beneficial effects of the present utility model are:

[0013] The utility model is convenient for storing large culture apparatuses separately by arranging partitions, mainly controlling the temperature of the outer side of the culture dish by arranging the culture box body, mainly conveniently blocking the culture box body by arranging the sealing component, and controlling the temperature of the inner cavity of the culture box body at the same time, limiting the bottom of the culture dish by arranging the reset holding component, limiting the top of the culture dish by arranging the top limiting component, and indirectly fixing the reset holding component. When in use, the cylinder drives the sealing door to move to the left side through the control panel, so as to open it, then the handle is moved upward, the positioning plate drives the sliding rod and the fixing rod to move upward, the fixing rod is separated from the inner cavity of the card slot, and then the slider drives the accommodating rack to move to the front under the action of the first spring, and after moving to the specified position, the packaged culture dish is placed in the inner cavity of the accommodating rack, and finally after the culture dish is installed, the reset holding component and the sealing component are reset, and the temperature inside the culture box body is adjusted and controlled through the control panel. The utility model has the advantages of convenient taking and placing and good fixing effect, and solves the problem that the culture dish of the prior art is not convenient to be fixed when in use, and the taking and placing is relatively troublesome. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a schematic diagram of the structure of the utility model;

[0015] Figure 2 This is a schematic diagram of the structure of the utility model from another perspective;

[0016] Figure 3 It is a schematic diagram of the left side structure of the utility model;

[0017] Figure 4 This is a schematic diagram of the structure of the reset holding component of the utility model;

[0018] Figure 5 It is a schematic diagram of the cross-sectional structure of the accommodating frame of the utility model when viewed from above.

[0019] In the figure: 1. incubator body; 2. sealing assembly; 21. sealing door; 22. control panel; 23. L-shaped mounting bracket; 24. cylinder; 25. positioning frame; 26. mounting seat; 3. reset holding assembly; 31. receiving frame; 32. slot; 33. slider; 34. first spring; 35. slide rail; 4. partition; 5. top limit assembly; 51. positioning plate; 52. sliding sleeve; 53. second spring; 54. sliding rod; 55. fixing rod. DETAILED DESCRIPTION

[0020] To make the objectives, technical solutions, and advantages of the embodiments of the present utility model clearer, the technical solutions in the embodiments of the present utility model will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are part of the embodiments of the present utility model, rather than all of the embodiments. Based on the embodiments in the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the scope of protection of the present utility model. Therefore, the following detailed description of the embodiments of the present utility model provided in the accompanying drawings is not intended to limit the scope of the present utility model to be protected, but merely represents the selected embodiments of the present utility model. Based on the embodiments in the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the scope of protection of the present utility model.

[0021] The present utility model provides a Figures 1-5 spore culture encapsulation device as shown, including a culture box body 1, a sealing component 2, a reset holding component 3, a partition 4, and a top limiting component 5. The partition 4 is fixedly installed at the bottom of the center of the inner cavity of the culture box body 1. The reset holding component 3 is arranged at the center of the inner cavity of the culture box body 1. The top limiting components 5 are arranged on both sides of the top of the reset holding component 3. The sealing component 2 is arranged on the left side of the culture box body 1. The sealing component 2 includes a sealing door 21, a control panel 22, an L-shaped mounting bracket 23, a cylinder 24, a positioning bracket 25, and a mounting seat 26. The reset holding component 3 includes a receiving frame 31, a card slot 32, a slider 33, a first spring 34, and a slide rail 35. The top limiting component 5 includes a positioning plate 51, a sliding sleeve 52, a second spring 53, a sliding rod 54, and a fixing rod 55. The mounting seats 26 are fixedly installed at the top and bottom of the left side of the front of the culture box body 1. The sealing door 21 rotates on the inner side of the mounting seat 26 through a rotating shaft. The L-shaped mounting bracket 23 is fixedly installed on the left side of the culture box body 1. The positioning bracket 25 is fixedly installed at one end of the left side of the sealing door 21. The cylinder 24 is movably connected to the left side of the positioning bracket 25 through a positioning pin. The slide rails 35 are fixedly installed on both sides of the inner cavity of the culture box body 1. The slider 33 slides in the inner cavity of the slide rail 35. The receiving frame 31 is fixedly installed on the relatively close side of the slider 33;

[0022] More specifically, by setting the top limit component 5 to limit the top of the petri dish, the reset holding component 3 is indirectly fixed. When in use, first, the control panel 22 is used to make the air cylinder 24 drive the sealing door 21 to move to the left, thereby opening it. Then, the handle is moved upward, and the positioning plate 51 drives the sliding rod 54 and the fixing rod 55 to move upward. The fixing rod 55 disengages from the inner cavity of the card slot 32. Immediately, under the action of the first spring 34, the slider 33 drives the accommodating rack 31 to move forward. After moving to the designated position, the encapsulated petri dish is placed in the inner cavity of the accommodating rack 31. Finally, after loading the petri dish, the reset holding component 3 and the sealing component 2 are reset, and the temperature inside the incubator body 1 is adjusted and controlled through the control panel 22, which has the advantages of convenient taking and placing and good fixing effect.

[0023] In some embodiments, the left side of the air cylinder 24 is rotatably connected to the inner cavity of the L-shaped mounting bracket 23 through a positioning pin. The first spring 34 is fixedly installed on both sides of the back of the accommodating rack 31. More specifically, the position of the sealing door 21 is moved by setting the air cylinder 24.

[0024] In some embodiments, the back of the first spring 34 is fixedly connected to the inner cavity of the incubator body 1. The card slots 32 are all opened on both sides inside the accommodating rack 31. More specifically, by setting the first spring 34, the accommodating rack 31 is mainly reset, so as to facilitate the taking and placing of the petri dish.

[0025] In some embodiments, the sliding sleeves 52 are all fixedly installed on both sides of the top center of the inner cavity of the incubator body 1. The sliding rod 54 slides in the inner cavity of the sliding sleeve 52. More specifically, by setting the sliding sleeve 52 to limit the sliding rod 54, it is prevented that the sliding rod 54 shakes during the moving process.

[0026] In some embodiments, the positioning plate 51 is fixedly installed at the bottom of the sliding rod 54. The second spring 53 is fixedly installed at the bottom of the sliding sleeve 52. More specifically, by setting the sliding rod 54 to limit the positioning plate 51, it is prevented that the positioning plate 51 shakes during the moving process.

[0027] In some embodiments, the bottom of the second spring 53 is fixedly connected to the top of the positioning plate 51. The fixing rods 55 are all fixedly installed on both sides of the bottom of the positioning plate 51. More specifically, by setting the second spring 53, the positioning plate 51 is reset.

[0028] In some embodiments, the control panel 22 is fixedly installed on the left side of the sealing door 21. A handle is fixedly installed on the top of the positioning plate 51. More specifically, by setting the fixing rod 55, it passes through the card slot 32 and penetrates into the inside of the accommodating rack 31, thereby fixing the accommodating rack 31.

[0029] Working principle:

[0030] When in use: First, through the control panel 22, the air cylinder 24 drives the sealing door 21 to move to the left, thus opening it. Then, move the handle upward. The positioning plate 51 drives the sliding rod 54 and the fixed rod 55 to move upward. The fixed rod 55 disengages from the inner cavity of the clamping groove 32. Immediately, under the action of the first spring 34, the slider 33 drives the accommodating rack 31 to move forward. After moving to the specified position, place the encapsulated petri dish in the inner cavity of the accommodating rack 31. Finally, after loading the petri dish, reset the resetting and containing assembly 3 and the sealing assembly 2, and adjust and control the temperature inside the incubator body 1 through the control panel 22, which has the advantages of convenient taking and placing and good fixing effect.

[0031] The above is only a preferred specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention, according to the technical solution of the present invention and its inventive concept, makes equivalent substitutions or changes, and all should be covered within the protection scope of the present invention.

[0032] It should be noted that in this article, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the term "comprising", "including" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements not only includes those elements, but also includes other elements not expressly listed, or further includes elements inherent to such process, method, article or device.

Claims

1. An encapsulation device for microspore culture, characterized in that: It includes an incubator body (1), a sealing component (2), a resetting and containing component (3), a partition plate (4) and a top limiting component (5). The partition plate (4) is fixedly installed at the bottom of the center of the inner cavity of the incubator body (1). The resetting and containing component (3) is arranged at the center of the inner cavity of the incubator body (1). The top limiting components (5) are arranged on both sides of the top of the resetting and containing component (3). The sealing component (2) is arranged on the left side of the incubator body (1). The sealing component (2) includes a sealing door (21), a control panel (22), an L-shaped mounting bracket (23), a cylinder (24), a positioning bracket (25) and a mounting seat (26). The resetting and containing component (3) includes a containing rack (31), a card slot (32), a slider (33), a first spring (34) and a slide rail (35). The top limiting component (5) includes a positioning plate (51), a sliding sleeve (52), a second spring (53), a sliding rod (54) and a fixing rod (55). The mounting seats (26) are fixedly installed at the top and bottom of the left side of the front of the incubator body (1). The sealing door (21) rotates on the inner side of the mounting seat (26) through a rotating shaft. The L-shaped mounting bracket (23) is fixedly installed on the left side of the incubator body (1). The positioning bracket (25) is fixedly installed at one end of the left side of the sealing door (21). The cylinder (24) is movably connected to the left side of the positioning bracket (25) through a positioning pin. The slide rails (35) are fixedly installed on both sides of the inner cavity of the incubator body (1). The slider (33) slides in the inner cavity of the slide rail (35). The containing rack (31) is fixedly installed on the relatively close side of the slider (33).

2. The encapsulation device for microspore culture according to claim 1, characterized in that: The left side of the cylinder (24) is rotationally connected to the inner cavity of the L-shaped mounting bracket (23) through a positioning pin. The first springs (34) are fixedly installed on both sides of the back of the containing rack (31).

3. The encapsulation device for microspore culture according to claim 1, characterized in that: The back of the first spring (34) is fixedly connected to the inner cavity of the incubator body (1). The card slots (32) are opened on both sides inside the containing rack (31).

4. The encapsulation device for microspore culture according to claim 1, characterized in that: The sliding sleeves (52) are fixedly installed on both sides of the top of the center of the inner cavity of the incubator body (1). The sliding rod (54) slides in the inner cavity of the sliding sleeve (52).

5. The encapsulation device for microspore culture according to claim 1, characterized in that: The positioning plate (51) is fixedly installed at the bottom of the sliding rod (54). The second spring (53) is fixedly installed at the bottom of the sliding sleeve (52).

6. The encapsulation device for microspore culture according to claim 1, characterized in that: The bottom of the second spring (53) is fixedly connected to the top of the positioning plate (51). The fixing rods (55) are fixedly installed on both sides of the bottom of the positioning plate (51).

7. The encapsulation device for microspore culture according to claim 1, characterized in that: The control panel (22) is fixedly installed on the left side of the sealing door (21). A handle is fixedly installed at the top of the positioning plate (51).

Images