Microorganism in-situ control culture device suitable for slow-flow water body
By designing a microbial in-situ control culture device suitable for slow-flowing water bodies, the problems of stability and condition control of microbial culture devices in the field environment in the prior art are solved, and precise control and flexible operation of microbial growth conditions in the field water environment are achieved.
Patent Information
- Application Number
- CN202421397399.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-19
- Publication Date
- 2025-07-11
- Estimated Expiration
- 2034-06-19
AI Technical Summary
The existing microbial culture methods are mainly limited to laboratory conditions, making it difficult to truly simulate the natural environment, and the existing in-situ culture devices are easily disturbed by external environment, cannot accurately control the growth conditions of microbials, and are difficult to meet the needs of field research.
A microbial in-situ controlled culture device suitable for slow-flowing water bodies is designed. It adopts a cylindrical tempered glass tube culture bottle, filter mesh and microporous glass fiber filter membrane, combined with floats, connecting ropes and settlement parts to form a stable limiting assembly, which can maintain the stability of the culture bottle in a field water environment, and increase multiple culture depths and control conditions through multiple connecting ropes.
It realizes precise control of microbial growth conditions in the field water environment, reduces shaking of the culture bottle, ensures the stability and flexibility of the device, is easy to carry and operate, and is suitable for simulation of a variety of culture conditions.
Smart Images
Figure CN223087822U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of in-situ microorganism cultivation, and specifically relates to a microorganism in-situ control cultivation device applicable to slow-flowing water bodies. Background Art
[0002] In the field of environmental ecological research, the role of microorganisms has been increasingly emphasized. They are key participants in biogeochemical cycles and play a crucial role in pollutant degradation, soil fertility improvement, and ecosystem services. However, despite significant progress in microbiological research, most microorganisms are still difficult to cultivate under laboratory conditions, resulting in our limited understanding of their functions and ecological mechanisms. With the continuous in-depth study of microbiology, the in-situ cultivation technology of microorganisms has become an important means to explore their functions and mechanisms. Therefore, it is particularly important to develop and apply the in-situ cultivation technology of microorganisms.
[0003] Currently, the main methods for microorganism cultivation include enrichment cultivation, pure culture in the laboratory, and controlled cultivation using bioreactors. However, traditional cultivation methods are often limited to laboratory conditions and are difficult to truly simulate the complexity of the natural environment, which restricts the understanding of the natural behavior and functions of microorganisms. Moreover, most existing in-situ cultivation devices adopt an open design, are easily interfered by external environmental factors, cannot precisely control the growth conditions of microorganisms, and are difficult to meet the needs of field research. Therefore, in order to better study the environmental processes and change characteristics of microorganisms in slow-flowing water bodies, it is particularly important to design a microorganism in-situ control cultivation device applicable to slow-flowing water bodies. Summary of the Utility Model
[0004] To solve the problems raised in the above background art, the purpose of the present utility model is to provide a microorganism in-situ control cultivation device applicable to slow-flowing water bodies.
[0005] To achieve the above purpose, the technical solution adopted by the present utility model is as follows:
[0006] A microbial in-situ control culture device applicable to slow-flowing water bodies, comprising: a culture unit and a limiting component. The culture unit includes a culture bottle, a filter screen, and a micro-porous glass fiber filter membrane; the limiting component includes a buoy, a connecting rope, a pulling rope, and a settling component. The culture bottle is a cylindrical tempered glass tube with openings at both the left and right ends, and a layer of filter screen with an optional pore size and a layer of micro-porous glass fiber filter membrane with a pore size that can be freely selected according to test requirements are provided at both ends. During use, the position of the culture unit and the connecting rope can be freely fixed to flexibly adjust the culture depth. After the settling component is fixed, the culture unit immediately reaches the designated culture depth. The material of the connecting rope should have characteristics such as high strength, corrosion resistance, and wear resistance; the material of the buoy should have characteristics such as corrosion resistance, anti-aging, light weight, large buoyancy, and bright color, and can be used for a long time in slow-flowing water bodies such as seawater and fresh water; the material of the settling component should have characteristics such as corrosion resistance and high density.
[0007] Further, a layer of filter screen with an optional pore size is provided on the outer sides of both the left and right ends of the culture bottle to isolate impurities such as sand grains and leaf residues in the water.
[0008] Further, a layer of micro-porous glass fiber filter membrane is provided on the inner sides of both the left and right ends of the culture bottle, and its pore size can be freely selected according to test requirements.
[0009] Further, the connecting rope is a multi-segment connection. The first segment of the connecting rope connects the buoy and the culture unit, and the second segment of the connecting rope connects the culture unit and the settling component; the culture unit can also be continuously connected to the lower end of the second segment of the connecting rope according to test requirements, and the last segment of the connecting rope connects the settling component to form several culture units with different culture depths.
[0010] Further, two pulling ropes are provided at the top and bottom of the culture bottle respectively. Both pulling ropes are in a triangular structure and are respectively fixed to the first and second segments of the connecting rope to form two stable triangular areas by being fixed to the connecting rope, thereby ensuring the stability of the culture bottle fixed on the connecting rope.
[0011] Further, the buoy is connected by the first segment of the connecting rope and floats on the water surface for observing the position of the culture bottle and collecting the culture bottle during the culture process; the settling component is connected by the last segment of the connecting rope and sinks into the sediment at the bottom of the water to fix the position of the culture bottle in the water flow; the stability of the culture bottle in the field water environment can be ensured by the combined action of the buoy and the settling component.
[0012] Further, during the in-situ culture experiment, the culture device can be provided with multiple groups of micro-porous glass fiber filter membranes with different pore sizes at the same time, and a single or multiple organic matters or pollutants can be added to each group.
[0013] Compared with the prior art, the superior effect of the culture device of the present utility model lies in:
[0014] 1. The culture bottle of the described culture device is a cylindrical tempered glass tube, which can effectively resist the impact of water flow and reduce the swaying of the culture bottle in water; moreover, the material has strong chemical stability and is extremely difficult to be corroded by seawater or damaged by biological behavior, ensuring the stability for long-term use in the wild water environment.
[0015] 2. The microporous glass fiber filter membrane in the described culture device can be freely selected or replaced according to test requirements, and several culture environments with different control conditions can be set simultaneously.
[0016] 3. The described culture device can increase multiple culture units by adding multiple connecting ropes, and multiple different culture depths can be set according to test requirements.
[0017] 4. The described culture device can greatly enhance the stability of the culture unit in the wild and complex water environment through the combined action of the buoy, pulling rope, connecting rope and settling piece.
[0018] 5. The described culture device has a simple structure and flexible assembly, is convenient to carry during field tests, and is easy to put in and retrieve, with a wide range of applicable scenarios and general applicability to slow-flowing water bodies. BRIEF DESCRIPTION OF THE DRAWINGS
[0019] Figure 1 It is a schematic structural diagram of a microorganism in-situ control culture device applicable to slow-flowing water bodies.
[0020] Figure 2 It is a schematic diagram of the influence of single or composite nutrient sources on the growth and function of microorganisms.
[0021] Figure 3 It is a schematic diagram of the influence of channels with different pore sizes on the growth and function of microorganisms.
[0022] Figure 4 It is a schematic diagram of the differences in the growth and function of microorganisms at different culture depths.
[0023] In the figure: 1. Buoy; 2. Connecting rope; 3. Pulling rope; 4. Culture bottle; 5. Filter screen; 6. Microporous glass fiber filter membrane; 7. Settling piece. DETAILED DESCRIPTION OF THE EMBODIMENTS
[0024] The following further details the described culture device of the present utility model in conjunction with the drawings and specific embodiments.
[0025] See the appendix Figure 1 , a microorganism in-situ control culture device applicable to slow-flowing water bodies of the present utility model includes a buoy 1, a connecting rope 2, a pulling rope 3, a culture bottle 4, a filter screen 5, a microporous glass fiber filter membrane 6, and a settling piece 7, as Figure 1As shown in the figure, the first - stage connecting rope is connected to the two pulling ropes 3 at the top of the buoy 1 and the culture bottle 4; the second - stage connecting rope is connected to the two pulling ropes 3 at the bottom of the sedimentation member 7 and the culture bottle 4. In the present utility model, the buoy 1, the connecting rope 2, the pulling rope 3 and the sedimentation member 7 together form a limiting component; the culture bottle 4, the filter screen 5, and the micro - porous glass fiber filter membrane 6 together form a culture unit. A layer of filter screen 5 is arranged on the outer sides of the left and right ends of the culture bottle 4 to isolate impurities such as sand grains and leaf residues in the water; a layer of micro - porous glass fiber filter membrane 6 with an aperture that can be freely selected according to test requirements is arranged on the inner sides of the left and right ends of the culture bottle 4. The buoy 1 is connected to the two pulling ropes 3 at the top of the culture bottle 4 through the first - stage connecting rope; the sedimentation member 7 is connected to the two pulling ropes 3 at the bottom of the culture bottle 4 through the second - stage connecting rope, thus forming an integral body. The two pulling ropes (3) are both in a triangular structure and are respectively fixed to the first - stage and second - stage connecting ropes, which can fix the culture bottle (4) to be in a horizontal direction to ensure the stability of the long - term use of the culture bottle (4) in water. In the specific implementation process of the present utility model, first, the sedimentation member 7 is connected by the connecting rope 2 and put into the water to measure the water depth, and then a connecting rope 2 with an appropriate length is selected according to test requirements to connect the culture device. During the process of putting the culture device into the water, the culture bottle 4 descends to the designated depth by relying on the gravity of the sedimentation member 7. At this time, the sedimentation member 7 sinks into the bottom sediment to fix its position, and the buoy 1 floats on the water surface to pull the culture bottle 4 and mark the position, so that long - term in - situ culture experiments in the wild can be carried out. The device of the present utility model can increase the number of culture bottles 4 at multiple different culture depths by adding multiple sections of connecting ropes 2; or the aperture of the micro - porous glass fiber filter membrane 6 on the inner sides of the left and right ends of the culture bottle 4 can be changed, and at the same time, culture environments with multiple different control conditions can be set. In the present utility model, the sedimentation member 7 should be made of a material with anti - corrosion and high density characteristics. The connecting rope 2 should be made of a material with high strength, corrosion resistance, and wear resistance characteristics. The buoy 1 should be made of a material with anti - corrosion, anti - aging, light weight, large buoyancy, and bright color characteristics, and can be used for a long time in slow - flowing water bodies such as seawater and fresh water.
[0026] Example 1: In - situ domestication test of microorganisms: To study the effects of single or composite nutrient sources on the growth and functions of microorganisms
[0027] See the appendix Figure 2, in this embodiment, three different types of nutrient sources are set and loaded into the culture bottles: ① single carbon source, ② single nitrogen source, ③ composite nutrient source. A 0.5-mm filter screen is provided on the outer sides of both left and right ends of the culture bottle to isolate impurities such as sand grains and leaf residues in the water body; a microporous glass fiber filter membrane with a pore size of 0.22 μm is provided on the inner sides of both left and right ends of the culture bottle to filter microorganisms and nutrients in the water body, etc., to ensure that the microorganisms cultured in the culture bottle are not interfered by other substances in the environmental water body. During the experiment of this embodiment, the two connecting ropes selected for the three groups of culture devices are of the same length, which can ensure that the three groups of culture bottles are at the same culture depth to study the effects of single nutrient source and composite nutrient source on the growth or function of microorganisms at the same culture depth.
[0028] Example 2. Microbial flow and exchange control experiment: To study the effects of channels with different pore sizes on the growth and function of microorganisms
[0029] See Appendix Figure 3 , in this embodiment, four groups of microporous glass fiber filter membranes with different pore sizes are respectively provided on the inner sides of both left and right ends of the culture bottle: ① control group, without setting microporous glass fiber filter membrane; ② the pore size of the microporous glass fiber filter membrane is 0.45 μm, which is used to filter impurities such as suspended substances in the water body, but does not limit the flow of microorganisms; ③ the pore size of the microporous glass fiber filter membrane is 0.22 μm, which is used to filter microorganisms in the water body, but does not limit the flow of nutrients; ④ the pore size of the microporous glass fiber filter membrane is 0.1 μm, which is used to filter nutrients in the water body to simulate the starvation control of microorganisms. And a 0.5-mm filter screen is provided on the outer sides of both left and right ends of the culture bottle to isolate impurities such as sand grains and leaf residues in the water body. During the experiment of this embodiment, the two connecting ropes selected for the four groups of culture devices are of the same length, which can ensure that the four groups of culture bottles are at the same culture depth to study the effects of the differences in the growth of microorganisms under the control of microporous glass fiber filter membranes with different pore sizes at the same culture depth.
[0030] Example 3. Microbial response difference experiment in different water layers: To study the differences in the growth and function of microorganisms at different culture depths
[0031] See Appendix Figure 4, in this embodiment, two groups of micro-porous glass fiber filter membranes with different pore diameters are respectively arranged on the inner sides of the left and right ends of the culture bottle: ① The pore diameter of the micro-porous glass fiber filter membrane is 0.45 μm, which is used to filter impurities such as suspended substances in the water body, but does not limit the flow of microorganisms; ② The pore diameter of the micro-porous glass fiber filter membrane is 0.22 μm, which is used to filter microorganisms in the water body, but does not limit the flow of nutrients. And a 0.5-mm filter screen is arranged on the outer sides of the left and right ends of the culture bottle to isolate impurities such as sand grains and leaf residues in the water body. During the test process of this embodiment, three connecting ropes are respectively selected for each culture device to connect two culture bottles and fix them at different culture depths to simulate the in-situ control culture of microorganisms in different water layers, so as to study the differences in the growth and functions of microorganisms at different culture depths.
[0032] Based on the above embodiments, the utility model is an in-situ control culture device for microorganisms in slow-flowing water bodies, which solves the limitations of traditional culture methods in the prior art, changes the single laboratory-condition culture mode, and truly simulates the complexity of the natural environment. And through the effective combination of the culture unit and the limiting component, the culture unit composed of the culture bottle 4, the filter screen 5 and the micro-porous glass fiber filter membrane 6 is limited within the limiting component, and through the action of the top buoy 1 and the bottom settling member 7, the fixed culture bottle 4 can be kept in the horizontal direction, reducing the shaking of the culture bottle 4 in the water. Moreover, a material with strong chemical stability is selected, which will not be corroded by seawater or damaged by biological behavior, and can ensure the stability of long-term use in the wild water environment.
[0033] Through the implementation mode of the utility model, the problem that the microorganism culture in the prior art is easily interfered by external environmental factors is changed, the growth conditions of microorganisms can be accurately controlled, and the device has a simple structure, flexible assembly, is convenient to carry during the field test, and is easy to put in and recycle, with a wide range of applicable scenarios and general applicability to slow-flowing water bodies.
[0034] It should be further noted that the above embodiments do not limit the structure of the utility model, and the provided embodiments are only used for understanding the technical solutions of the present invention. It can be determined that according to the technical concept of the utility model, there will be other more choices for the specific application modes of the device, and the utility model does not cover other implementation modes in the above embodiments. Any obvious modification or improvement to the structure that belongs to the technical concept of the utility model shall fall within the protection scope of the utility model.
Claims
1. An in-situ microbial control and culture device applicable to slow-flow water bodies, characterized in that, Including: A culture unit and a limiting component. The culture unit includes a culture bottle (4), a filter screen (5), and a microporous glass fiber filter membrane (6); the limiting component includes a buoy (1), a connecting rope (2), a pulling rope (3), and a settling component (7); the material of the connecting rope (2) should have the characteristics of high strength, corrosion resistance, and wear resistance. The first section of the connecting rope is connected to the two pulling ropes (3) at the top of the buoy (1) and the culture bottle (4); the second section of the connecting rope is connected to the two pulling ropes (3) at the bottom of the settling component (7) and the culture bottle (4). The first section of the connecting rope connects the buoy (1) and the culture bottle (4) through the pulling rope (3); the second section of the connecting rope connects the culture bottle (4) and the settling component (7) through the pulling rope (3).
2. The in-situ microbial control and culture device applicable to slow-flow water bodies according to claim 1, characterized in that: The buoy (1) is connected to the pulling rope (3) at the top of the culture bottle (4) through the first section of the connecting rope. The material of the buoy (1) should have the characteristics of corrosion resistance, anti-aging, light weight, large buoyancy, and bright color, and can be used for a long time in slow-flowing seawater and fresh water bodies.
3. The in-situ microbial control and cultivation device applicable to slow-flow water bodies according to claim 1, wherein: Both the top and bottom of the culture bottle (4) have two pulling ropes (3). The two pulling ropes (3) are both in a triangular structure and are respectively fixed to the first and second sections of the connecting rope, which can fix the culture bottle (4) to keep it in the horizontal direction to ensure the stability of the culture bottle (4) during long-term use in water.
4. The in-situ microbial control and culture device applicable to slow-flow water bodies according to claim 1, characterized in that: The culture bottle (4) is a cylindrical tempered glass tube with open designs at both left and right ends, and a layer of filter screen (5) and a layer of microporous glass fiber filter membrane (6) are provided at both ends.
5. The in-situ microbial control and cultivation device applicable to slow-flow water bodies according to claim 4, characterized in that: A layer of filter screen (5) with an optional pore size is provided on the outer sides of both left and right ends of the culture bottle (4) to isolate floating impurities in the water.
6. The in-situ microbial control and cultivation device applicable to slow-flow water bodies according to claim 4, characterized in that: A layer of microporous glass fiber filter membrane (6) with an optional pore size is provided on the inner sides of both left and right ends of the culture bottle (4).
7. The in-situ microbial control and cultivation device applicable to slow-flow water bodies according to claim 1, wherein: The material of the settling component (7) should have the characteristics of corrosion resistance and high density. The settling component (7) is connected to the pulling rope (3) at the bottom of the culture bottle (4) through the second section of the connecting rope.