Semi-automatic in-vitro follicular fluid collecting device

Abstract

The utility model discloses an in-vitro follicular fluid semi-automatic collecting device which comprises a negative pressure adsorption assembly, the negative pressure adsorption assembly comprises a portable liquid suction device, a waste liquid cup is detachably arranged on one side of the portable liquid suction device, the portable liquid suction device is connected with the waste liquid cup through a suction pipe, and the suction pipe is connected with the negative pressure adsorption assembly. A suction catheter is arranged at the upper end of the waste liquid cup, one end of the suction catheter extends into the waste liquid cup, and the other end of the suction catheter is fixedly connected with a three-way valve; and the connecting pipe group comprises an exhaust extension hose. According to the in-vitro follicular fluid semi-automatic collecting device, the negative pressure adsorption assembly, the connecting pipe set and the sampling needle set are combined, follicular fluid can be extracted in a semi-automatic mode, a large amount of follicular fluid needs to be collected, for example, when the follicular fluid exceeds 200 mL, the follicular fluid can be collected by multiple persons through the same instrument at the same time, materials are used as least as possible, and the cost is reduced. The sampling speed is increased, and the follicular fluid collection efficiency and quality are guaranteed.

Description

Technical Field

[0001] The utility model relates to the technical field of reproductive medicine, and particularly relates to a semi-automatic collecting device for in vitro follicular fluid. Background Art

[0002] The collection of animal follicular fluid is a part of the basic research of reproductive medicine. By collecting the follicular fluid in animal ovaries, animal oocytes, granulosa cells, and rich components in the follicular fluid such as bioactive substances like estrogen, progesterone, exosomes, etc. can be collected to carry out research on ovarian function, reproductive physiology, and reproductive toxicology. Currently, the most commonly used methods for collecting follicular fluid in the laboratory are mainly syringes or using a vacuum pump for aspiration. When collecting follicular fluid in laboratory work, the collection speed of follicular fluid is the key to affecting the cell viability in subsequent cell experiments.

[0003] When using the syringe aspiration method, the collector needs to hold the ovary with one hand and the syringe with the other hand, and collect the follicular fluid in the ovary through the negative pressure generated by aspirating the syringe. It is necessary to continuously pull and draw the syringe. When a certain amount of follicular fluid is collected, it is transferred to a new EP tube, and the target sample is collected according to the requirements of subsequent experiments. This method takes a long time and the amount of follicular fluid obtained is small. Especially when collecting small follicular fluid, a large amount of small follicular fluid is often lost due to the poor negative pressure effect of the syringe, resulting in less follicular fluid obtained from the same number of ovaries.

[0004] When using a vacuum pump for aspiration, although the efficiency is improved to a certain extent compared with the syringe aspiration method, the operation is not convenient, and the same device cannot be used for multiple people to collect follicular fluid simultaneously. When a large amount of fresh follicular fluid needs to be collected for subsequent experiments, it takes a long time and the efficiency cannot be improved.

[0005] Therefore, this application proposes a semi-automatic collecting device for in vitro follicular fluid, and provides a new technical solution to solve the above-mentioned technical problems. Summary of the Utility Model

[0006] Based on this, it is necessary to provide a semi-automatic collecting device for in vitro follicular fluid for the above-mentioned technical problems. By combining a negative pressure adsorption component, a connecting pipe group, and a sampling needle group, semi-automatic extraction of follicular fluid can be realized. When a large amount of follicular fluid needs to be collected, such as more than 200 ml of follicular fluid, multiple people can collect it simultaneously with the same instrument, and the sampling speed can be accelerated with as few materials as possible, which is beneficial to ensuring the collection efficiency and quality of follicular fluid.

[0007] In order to solve the above technical problems, the utility model adopts the following technical solutions:

[0008] A semi-automatic collecting device for in vitro follicular fluid is applied to the collection of in vitro follicular fluid.

[0009] The described semi-automatic in vitro follicular fluid collection device specifically includes:

[0010] A negative pressure adsorption assembly, the negative pressure adsorption assembly includes a portable liquid suction device, a waste liquid cup is detachably arranged on one side of the portable liquid suction device, the portable liquid suction device is connected with the waste liquid cup through a suction tube, a suction guide tube is further arranged at the upper end of the waste liquid cup, one end of the suction guide tube extends into the waste liquid cup, and the other end of the suction guide tube is fixedly connected with a three-way valve;

[0011] A connecting pipe group, which includes an exhaust extension hose, one end of the exhaust extension hose is connected with the three-way valve, the other end of the exhaust extension hose is fixedly connected with an adapter, a connecting insertion tube is arranged on the adapter, an exhaust connecting pipe is inserted and connected to the connecting insertion tube, and the other end of the exhaust connecting pipe is connected with a follicular fluid collection device;

[0012] A sampling needle group, which includes a sampling needle connecting pipe, sampling needle bodies are fixedly connected to both ends of the sampling needle connecting pipe, the sampling needle connecting pipe is connected with the follicular fluid collection device through the sampling needle body at one end, and in vitro follicular fluid sampling is carried out through the sampling needle body at the other end.

[0013] As a preferred embodiment of the semi-automatic in vitro follicular fluid collection device provided by the present invention, two connecting insertion tubes are arranged on the adapter, each connecting insertion tube is separately connected with a follicular fluid collection device through an exhaust connecting pipe, and a sampling needle group is connected to each follicular fluid collection device.

[0014] As a preferred embodiment of the semi-automatic in vitro follicular fluid collection device provided by the present invention, the number of the three-way valves is multiple, every two adjacent three-way valves are connected in series, and each three-way valve can be connected with an exhaust extension hose.

[0015] As a preferred embodiment of the semi-automatic in vitro follicular fluid collection device provided by the present invention, a sleeve rack is fixed on the side surface of the portable liquid suction device, the waste liquid cup is located inside the sleeve rack, and the waste liquid cup is connected with the portable liquid suction device through the sleeve rack.

[0016] As a preferred embodiment of the semi-automatic in vitro follicular fluid collection device provided by the present invention, the sampling needle body is a disposable venous blood sample collection needle.

[0017] As a preferred embodiment of the semi-automatic in vitro follicular fluid collection device provided by the present invention, it further includes a placement rack, and multiple follicular fluid collection devices can be placed in the placement rack.

[0018] Compared with the prior art, the utility model has the following beneficial effects:

[0019] The semi-automatic in vitro follicular fluid collection device provided by the utility model can realize semi-automatic extraction of follicular fluid by combining a negative pressure adsorption component, a connecting pipe group and a sampling needle group; and when a large amount of follicular fluid needs to be collected, such as more than 200 ml of follicular fluid, multiple people can collect it simultaneously with the same instrument, using the least amount of materials as possible to speed up the sampling speed, which is beneficial to ensuring the collection efficiency and quality of follicular fluid. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the solutions in the present utility model, the following will briefly introduce the drawings required for use in the description of the embodiments. Obviously, the following drawings are some embodiments of the present utility model. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0021] Figure 1 It is a schematic diagram of the overall structure of the semi-automatic in vitro follicular fluid collection device provided by the present utility model;

[0022] Figure 2 It is a schematic diagram of the structure of the connecting pipe group and the sampling needle group of the semi-automatic in vitro follicular fluid collection device provided by the present utility model;

[0023] Figure 3 It is a schematic diagram of the structure of multiple connecting pipe groups and sampling needle groups of the semi-automatic in vitro follicular fluid collection device provided by the present utility model;

[0024] Figure 4 It is a schematic diagram of the structure of multiple connecting pipe groups and follicular fluid collection equipment of the semi-automatic in vitro follicular fluid collection device provided by the present utility model;

[0025] Figure 5 It is a schematic diagram of the structure of the sampling needle group and the follicular fluid collection equipment of the semi-automatic in vitro follicular fluid collection device provided by the present utility model.

[0026] The label descriptions in the figures are as follows:

[0027] 1. Portable liquid suction device; 2. Waste liquid cup; 3. Suction tube; 4. Suction catheter; 5. Three-way valve; 6. Exhaust extension hose; 7. Adapter; 8. Exhaust connection pipe; 9. Follicular fluid collection equipment; 10. Sampling needle connection pipe; 11. Sampling needle body; 12. Sleeve holder; 13. Connecting insertion tube; 14. Spiral cap. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0028] To enable those skilled in the art to better understand the solution of the present utility model, the following will clearly and completely describe the technical solutions in the embodiments of the present utility model in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all of the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model.

[0029] As described in the background art, when using the syringe aspiration method, the collector needs to hold the ovary with one hand and hold the syringe with the other hand, and collect the follicular fluid in the ovary through the negative pressure generated by aspirating the syringe. It is necessary to continuously pull and draw the syringe. When a certain amount of follicular fluid is collected, it is transferred to a new EP tube, and the target sample is collected according to the subsequent experimental requirements. This method takes a long time and the amount of follicular fluid obtained is small. Especially when collecting small follicular fluid, a large amount of small follicular fluid is often lost due to the poor negative pressure effect of the syringe, resulting in less follicular fluid obtained from the same number of ovaries.

[0030] When using a vacuum pump for aspiration, although the efficiency is improved to a certain extent compared with the syringe aspiration method, the operation is not convenient, and the same device cannot be used for multiple people to collect follicular fluid simultaneously. When a large amount of fresh follicular fluid needs to be collected for subsequent experiments, it takes a long time and the efficiency cannot be improved.

[0031] To solve this technical problem, the present utility model provides a semi-automatic in vitro follicular fluid collection device, which is applied to the collection of in vitro follicular fluid.

[0032] Specifically, please refer to Figure 1 - Figure 5 , the semi-automatic in vitro follicular fluid collection device specifically includes:

[0033] A negative pressure adsorption assembly, the negative pressure adsorption assembly includes a portable liquid suction device. A waste liquid cup is detachably arranged on one side of the portable liquid suction device. The portable liquid suction device is connected to the waste liquid cup through a suction tube. A suction guide tube is also arranged at the upper end of the waste liquid cup. One end of the suction guide tube extends into the interior of the waste liquid cup, and the other end of the suction guide tube is fixedly connected to a three-way valve;

[0034] A connecting tube group, which includes an exhaust extension hose. One end of the exhaust extension hose is connected to the three-way valve, and the other end of the exhaust extension hose is fixedly connected to a connector. A connecting insertion tube is arranged on the connector, and an exhaust connecting tube is inserted and connected to the connecting insertion tube. The other end of the exhaust connecting tube is connected to a follicular fluid collection device;

[0035] Sampling needle group, which includes a sampling needle connecting tube. Both ends of the sampling needle connecting tube are fixedly connected with sampling needle bodies. One end of the sampling needle connecting tube is connected to the follicular fluid collection device through the sampling needle body, and the other end of the sampling needle connecting tube is used for in vitro follicular fluid sampling through the sampling needle body.

[0036] The semi-automatic in vitro follicular fluid collection device provided by the present utility model can achieve semi-automatic extraction of follicular fluid by combining a negative pressure adsorption component, a connecting tube group, and a sampling needle group. When a large amount of follicular fluid needs to be collected, such as more than 200 ml of follicular fluid, multiple people can use the same instrument to collect simultaneously, using as few materials as possible to speed up the sampling speed, which is beneficial to ensuring the collection efficiency and quality of follicular fluid.

[0037] In order to enable those skilled in the art to better understand the solution of the present utility model, the technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings.

[0038] Embodiment 1:

[0039] Please refer to Figure 1 - Figure 5 , a semi-automatic in vitro follicular fluid collection device, which includes:

[0040] Negative pressure adsorption component, the negative pressure adsorption component includes a portable liquid suction device 1. A waste liquid cup 2 is detachably arranged on one side of the portable liquid suction device 1. Among them, a sleeve 12 is fixed on the side of the portable liquid suction device 1. The waste liquid cup 2 is located inside the sleeve 12. The waste liquid cup 2 is connected to the portable liquid suction device 1 through the sleeve 12. The portable liquid suction device 1 is connected to the waste liquid cup 2 through a suction tube 3. An inhalation catheter 4 is also arranged at the upper end of the waste liquid cup 2. One end of the inhalation catheter 4 extends into the waste liquid cup 2, and the other end of the inhalation catheter 4 is fixedly connected to a three-way valve 5;

[0041] Connecting tube group, which includes an exhaust extension hose 6. One end of the exhaust extension hose 6 is connected to the three-way valve 5, and the other end of the exhaust extension hose 6 is fixedly connected to an adapter 7. A connecting insertion tube 13 is arranged on the adapter 7, and an exhaust connecting tube 8 is inserted and connected to the connecting insertion tube 13. The other end of the exhaust connecting tube 8 is connected to a follicular fluid collection device 9;

[0042] Sampling needle group, which includes a sampling needle connecting tube 10. Both ends of the sampling needle connecting tube 10 are fixedly connected with sampling needle bodies 11. One end of the sampling needle connecting tube 10 is connected to the follicular fluid collection device 9 through the sampling needle body 11, and the other end of the sampling needle connecting tube 10 is used for in vitro follicular fluid sampling through the sampling needle body 11. Among them, the sampling needle body 11 is a disposable venous blood sample collection needle.

[0043] Further, two connecting cannulas 13 are provided on the adapter 7. Each connecting cannula 13 is separately connected to a follicular fluid collection device 9 through an exhaust connecting tube 8, and a sampling needle group is connected to each follicular fluid collection device 9. The connecting cannula 13 is equipped with a detachable screw cap 14. When only one connecting cannula 13 needs to be used, cover the other connecting cannula 13 with the screw cap 14 to prevent air leakage from affecting the use (such as Figure 1 and 2 ); when both connecting cannulas 13 need to be connected to the exhaust connecting tube 8 for use, then unscrew the screw cap 14, connect the connecting cannula 13 to the exhaust connecting tube 8, then the exhaust connecting tube 8 is connected to the follicular fluid collection device 9, and the follicular fluid collection device 9 is connected to the sampling needle group, and then the follicular fluid can be collected (such as Figure 3 and 4 ).

[0044] Working principle: The portable liquid suction device 1 can replace the negative pressure generated by manually sucking a syringe to collect follicular fluid in the ovary, and can realize semi-automatic operation, which can not only save manpower but also improve efficiency. The three-way valve 5 can be connected to multiple exhaust extension hoses 6 as needed through the suction conduit 4 of the portable liquid suction device 1. There are three connections in the connecting pipe group. The first connection of the connecting pipe group is the connection between the exhaust extension hose 6 and the three-way valve 5; the second connection of the connecting pipe group is the connection between the exhaust extension hose 6 and the adapter 7, which is detachable according to the use requirements; the third connection of the connecting pipe group is the connection between the exhaust connecting tube 8 and the follicular fluid collection device 9. During operation, it is often difficult to work in a limited laminar flow bench because the distance between the portable liquid suction device 1 and the operating table is too far and the instrument occupies a large area. Using the connecting pipe group is beneficial to expanding the use area of the laminar flow bench. At the same time, the adapter 7 in the exhaust connecting pipe group has two connecting cannulas 13, and the two connecting cannulas 13 can be connected to two exhaust connecting tubes 8 at the same time, or only one exhaust connecting tube 8 can be connected, so that one or two people can use it at the same time, and it can be selected according to the sampling needs, thereby improving the sampling efficiency. The sampling needle connecting tube 10 is connected to the sampling needle body 11 of a disposable venous blood sample collection needle at both ends, one end is for sampling, and the other end is connected to the follicular fluid collection device 9. During the whole sampling process, after assembling the sampling needle connecting tube 10, the three-way valve 5, the exhaust extension hose 6, the follicular fluid collection device 9 and the portable liquid suction device 1, turn on the switch, and then the portable liquid suction device 1 can be turned on to generate negative pressure in the follicular fluid collection device 9 through the connecting pipe group, so that the negative pressure can be used to perform semi-automatic follicular fluid aspiration and collection through the sampling needle connecting tube 10.

[0045] Example 2:

[0046] The in vitro follicular fluid semi-automatic collection device provided in Example 1 is further optimized. Specifically, as Figures 2 - 4As shown, the number of three-way valves 5 is multiple, and every two adjacent three-way valves 5 are connected in series, and each three-way valve 5 can be connected to an exhaust extension hose 6.

[0047] Through the above structural design, the three-way valve 5 can be connected to multiple exhaust extension hoses 6, improving the usage efficiency of the instrument. When a large amount of follicular fluid needs to be collected, such as more than 200 ml of follicular fluid, multiple people can use the same instrument to collect at the same time, using the least amount of materials possible to improve the sampling rate. At the same time, by combining with a liquid suction system, this device can achieve semi-automatic extraction of follicular fluid, accelerating the sampling speed and being beneficial to ensuring the collection efficiency and quality of follicular fluid.

[0048] Embodiment 3:

[0049] The semi-automatic in vitro follicular fluid collection device provided in Embodiment 1 is further optimized. Specifically, it further includes a placement rack (not shown), and multiple follicular fluid collection devices 9 can be placed in one placement rack; through the above structural design, the placement of the follicular fluid collection devices 9 is relatively stable.

Claims

1. A semi-automatic device for collecting follicular fluid in vitro, characterized in that, Comprising: A negative pressure adsorption assembly, the negative pressure adsorption assembly includes a portable liquid suction device (1), a waste liquid cup (2) is detachably arranged on one side of the portable liquid suction device (1), the portable liquid suction device (1) is connected to the waste liquid cup (2) through a suction tube (3), a suction guide tube (4) is further arranged at the upper end of the waste liquid cup (2), one end of the suction guide tube (4) extends into the waste liquid cup (2), and the other end of the suction guide tube (4) is fixedly connected to a three-way valve (5); A connecting pipe group, which includes an exhaust extension hose (6), one end of the exhaust extension hose (6) is connected to the three-way valve (5), the other end of the exhaust extension hose (6) is fixedly connected to an adapter (7), a connecting insertion tube (13) is arranged on the adapter (7), an exhaust connecting pipe (8) is inserted and connected to the connecting insertion tube (13), and the other end of the exhaust connecting pipe (8) is connected to a follicular fluid collection device (9); A sampling needle group, which includes a sampling needle connecting pipe (10), sampling needle bodies (11) are fixedly connected to both ends of the sampling needle connecting pipe (10), the sampling needle connecting pipe (10) is connected to the follicular fluid collection device (9) through the sampling needle body (11) at one end, and in vitro follicular fluid sampling is performed through the sampling needle body (11) at the other end of the sampling needle connecting pipe (10).

2. The semi-automatic in vitro follicular fluid collection device according to claim 1, wherein Two connecting insertion tubes (13) are arranged on the adapter (7), each connecting insertion tube (13) is separately connected to a follicular fluid collection device (9) through an exhaust connecting pipe (8), and a sampling needle group is connected to each follicular fluid collection device (9).

3. The semi-automatic in vitro follicular fluid collection device according to claim 1, wherein, The number of the three-way valves (5) is multiple, every two adjacent three-way valves (5) are connected in series, and each three-way valve (5) can be connected to an exhaust extension hose (6).

4. The semi-automatic in vitro follicular fluid collection device according to claim 1, characterized in that, A sleeve holder (12) is fixed on the side of the portable liquid suction device (1), the waste liquid cup (2) is located inside the sleeve holder (12), and the waste liquid cup (2) is connected to the portable liquid suction device (1) through the sleeve holder (12).

5. The semi-automatic follicular fluid collection device in vitro according to claim 1, characterized in that, The sampling needle body (11) is a disposable venous blood sample collection needle.

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