Special culture dish for cell scratch experiment
By designing a special culture dish for cell scratch experiments and optimizing the coordination between the scratch frame and the scraper with mechanical structure, the operation complexity and cell fluid collection problems in traditional cell scratch experiments are solved, and the high accuracy and reliability of the experimental results are achieved, reducing the experimental cost.
Patent Information
- Application Number
- CN202422109288.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-29
- Publication Date
- 2025-07-11
- Estimated Expiration
- 2034-08-29
AI Technical Summary
Traditional cell scratch experiments are complicated, and it is difficult to ensure uniformity and depth consistency of scratches. The experimental conditions are inconsistent, and cell fluid is difficult to effectively collect, which affects the accuracy and repeatability of experimental results.
A special culture dish for cell scratch experiments was designed, including scratch frame, linkage handle, card block, slot, linkage block, telescopic spring, slide chute, linkage rod, scraper, telescopic rod, linkage spring, sealing ring and feeding groove. Through the coordination between the card block and the slot, the stability and precise positioning of the scratch frame are ensured, and the scraping amount of cell fluid is controlled by using the telescopic rod and linkage spring, and the sealing ring and feeding groove are combined to achieve efficient collection of cell fluid.
It improves the accuracy, stability and operational convenience of cell scratch experiments, ensures the accuracy and repeatability of experimental results, reduces material waste, and reduces experimental costs.
Smart Images
Figure CN223087835U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of culture dishes, and more specifically, to a culture dish dedicated to cell scratch experiments. Background Art
[0002] The cell scratch experiment, also known as the wound healing experiment, is a commonly used in vitro experiment method in cell biology research. It is mainly used to study biological processes such as cell migration, proliferation, and cell-cell interactions, and is of great significance especially in the fields of wound healing, tumor invasion, tissue regeneration, etc. In traditional cell scratch experiments, they are usually carried out on conventional culture dishes. The operator needs to manually use tools such as pipette tips or needles to create "scratches" on the cell monolayer in a sterile environment, and then observe the process of cell migration to fill the scratches through a microscope.
[0003] In the prior art, during the use of culture dishes:
[0004] (1) Operational complexity: Manual scratching operations rely on the skills and experience of the operator, making it difficult to ensure the uniformity and depth consistency of the scratches, which affects the repeatability of experimental results;
[0005] (2) Experimental condition control: Traditional methods are difficult to precisely control the width, depth, and the amount of cell fluid scraped, resulting in inconsistencies in experimental conditions and affecting the accuracy and reliability of experimental results;
[0006] (3) Waste liquid collection: In traditional experiments, the scraped cell fluid is often difficult to effectively collect, causing waste of experimental materials and affecting the progress of the cell scratch experiment; Therefore, we made improvements and proposed a culture dish dedicated to cell scratch experiments. Summary of the Utility Model
[0007] The purpose of the present utility model is to address the problems of operational complexity, unstable experimental condition control, and inability to quickly collect cell fluid in the current design of culture dishes.
[0008] To achieve the above-mentioned utility model purpose, the present utility model provides the following technical solutions:
[0009] A culture dish dedicated to cell scratch experiments to improve the above problems.
[0010] Specifically, this application is as follows:
[0011] A culture dish dedicated for cell scratch assay, comprising a culture dish, with a scratch frame provided at the inner end of the culture dish. Linkage handles are fixed at both ends of the scratch frame. A clamping block is fixed at the lower end of the linkage handle. A card slot is provided at the outer end of the clamping block. A linkage block and a telescopic spring are provided at the inner end of the card slot. A sliding groove is provided at the inner end of the scratch frame. A linkage rod is provided in the sliding groove. A scraping plate is provided at the inner end of the linkage rod. Telescopic rods and linkage springs are provided at both ends of the scraping plate. An embedded groove and an embedding block are provided between the scraping plate and the linkage rod. A sealing ring is provided at the lower end of the scratch frame. A bottom opening is provided between the scratch frame and the culture dish. A material discharge groove is provided at the outer end of the bottom opening.
[0012] As a preferred technical solution of the present application, the card slot wraps around the outer end of the clamping block. The linkage block moves up and down along the card slot, and there is a movable connection between the lower end of the linkage block and the telescopic spring.
[0013] As a preferred technical solution of the present application, a gravity block is provided inside the clamping block. The sliding groove is embedded and installed at the inner end of the scratch frame. The linkage rod slides left and right along the inner part of the sliding groove. A through groove is provided at the center of the linkage rod. The scraping plate moves up and down along the through groove.
[0014] As a preferred technical solution of the present application, the two ends of the telescopic rod are respectively fixed at the upper end of the scraping plate and the upper end of the linkage rod. There is a movable connection between the inner end of the telescopic rod and the linkage spring.
[0015] As a preferred technical solution of the present application, the embedded groove is embedded inside the narrow side of the scraping plate. The embedding block is fixed at the inner end of the narrow side of the through groove. There is a fixed connection between the bottom of the scratch frame and the sealing ring.
[0016] As a preferred technical solution of the present application, the material discharge groove is embedded at the bottom end of the culture dish. There is a through connection between the bottom opening and the material discharge groove. A through hole is provided at the upper end of the material discharge groove, and the through hole penetrates the outer surfaces of the upper and lower ends of the scratch frame.
[0017] As a preferred technical solution of the present application, scale lines are arranged at the upper end of the scratch frame.
[0018] As a preferred technical solution of the present application, a pressing plate is provided at the upper end of the scraping plate.
[0019] Compared with the prior art, the beneficial effects of the present utility model are:
[0020] In the solution of the present application:
[0021] The culture dish dedicated for cell scratch assay ensures the precise positioning and stability of the scratch frame in the culture dish through the cooperation of the clamping block and the card slot and the stable design of the gravity block and the linkage block;
[0022] The precise cooperation of the telescopic rod, the linkage spring and the embedded groove enables the scraping plate to accurately control the scraping amount of the cell fluid, improving the accuracy and repeatability of the experiment;
[0023] The design of the sealing ring and the bottom opening effectively prevents the leakage of cell fluid, and cooperates with the feeding groove to achieve efficient collection of materials;
[0024] The overall design aims to significantly improve the accuracy, stability and operation convenience of the cell scratch experiment through the optimization of the mechanical structure, providing a powerful tool for the research of cell migration and regeneration. Brief Description of the Drawings
[0025] Figure 1 It is a schematic diagram of the overall structure of the special culture dish for cell scratch experiment provided by this application;
[0026] Figure 2 It is a front sectional view of the special culture dish for cell scratch experiment provided by this application;
[0027] Figure 3 It is the Figure 2 amplified schematic diagram of A in the special culture dish for cell scratch experiment provided by this application;
[0028] Figure 4 It is a side sectional view of the special culture dish for cell scratch experiment provided by this application
[0029] Figure 5 It is the Figure 4 amplified schematic diagram of B in the special culture dish for cell scratch experiment provided by this application;
[0030] Figure 6 It is the Figure 4 amplified schematic diagram of C in the special culture dish for cell scratch experiment provided by this application;
[0031] Figure 7 It is a schematic diagram of the cross-sectional top view of the card slot of the special culture dish for cell scratch experiment provided by this application.
[0032] Labels in the figure:
[0033] 1. Culture dish; 2. Scratch frame; 3. Linkage handle; 4. Clamping block; 5. Card slot; 6. Linkage block; 7. Telescopic spring; 8. Feeding groove; 9. Sealing ring; 10. Slide groove; 11. Linkage rod; 12. Scraper; 13. Telescopic rod; 14. Linkage spring; 15. Bottom opening; 16. Embedded groove; 17. Embedded block. Detailed Embodiment
[0034] To make the objectives, technical solutions and advantages of the embodiments of the present utility model clearer, the technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the drawings. Obviously, the described embodiments are some, but not all, of the embodiments of the present utility model.
[0035] Therefore, the following detailed description of the embodiments of the present utility model is not intended to limit the scope of the claimed present utility model, but merely represents some embodiments of the present utility model. All other embodiments obtained by those of ordinary skill in the art based on the embodiments in the present utility model without creative efforts belong to the scope of protection of the present utility model. It should be noted that, without conflict, the embodiments in the present utility model and the features and technical solutions in the embodiments can be combined with each other.
[0036] It should be noted that like reference numerals and letters indicate similar items in the following figures. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.
[0037] As Figure 1-7 shown, this embodiment provides a special culture dish for cell scratch experiments, including a culture dish 1. A scratch frame 2 is provided at the inner end of the culture dish 1. Linkage handles 3 are fixed at both ends of the scratch frame 2. A clamping block 4 is fixed at the lower end of the linkage handle 3. A clamping groove 5 is provided at the outer end of the clamping block 4. A linkage block 6 and a telescopic spring 7 are provided at the inner end of the clamping groove 5. A sliding groove 10 is provided at the inner end of the scratch frame 2. A linkage rod 11 is provided at the inner end of the sliding groove 10. A scraping plate 12 is provided at the inner end of the linkage rod 11. Telescopic rods 13 and linkage springs 14 are provided at both ends of the scraping plate 12. An embedded groove 16 and an embedded block 17 are provided between the scraping plate 12 and the linkage rod 11. A sealing ring 9 is provided at the lower end of the scratch frame 2. A bottom opening 15 is provided between the scratch frame 2 and the culture dish 1. A feeding groove 8 is provided at the outer end of the bottom opening 15.
[0038] The clamping groove 5 wraps around the outer end of the clamping block 4. The linkage block 6 moves up and down along the clamping groove 5. The lower end of the linkage block 6 is movably connected to the telescopic spring 7.
[0039] The linkage handles 3 at both ends of the scratch frame 2, and the clamping block 4 at the lower end cooperate with the clamping groove 5 outside the culture dish 1 to ensure the stability of the scratch frame 2, guarantee the stability of the scratch frame 2 during the experiment, and avoid uneven scratches or experimental failures caused by external forces or improper operations.
[0040] A gravity block is provided inside the clamping block 4. The sliding groove 10 is embedded and installed at the inner end of the scratch frame 2. The linkage rod 11 slides left and right along the sliding groove 10. A through groove is provided at the center of the linkage rod 11. The scraping plate 12 moves up and down along the through groove.
[0041] The movable connection between the gravity block inside the clamping block 4 and the linkage block 6 uses the weight of the gravity block to stabilize the scratch frame 2, automatically adjusts using physical principles, ensures the verticality and stability of the scratch frame 2, and reduces human error.
[0042] The two ends of the telescopic rod 13 are respectively fixed to the upper end of the scraping plate 12 and the upper end of the linkage rod 11. The inner end of the telescopic rod 13 is movably connected to the linkage spring 14.
[0043] The scraping plate 12 is connected to the linkage rod 11 through the telescopic rod 13. When the scraping plate 12 moves up and down, the telescopic rod 13 and the linkage spring 14 act synchronously to accurately control the scraping amount of the cell fluid, ensuring the repeatability and accuracy of the experiment.
[0044] The embedded groove 16 is embedded inside the narrow side of the scraping plate 12, the embedded block 17 is fixed at the inner end of the narrow side of the through groove, and a fixed connection is provided between the bottom of the scratch frame 2 and the sealing ring 9.
[0045] The design of the embedded groove 16 and the embedded block 17 between the scraping plate 12 and the linkage rod 11 ensures the stability of the scraping plate 12 during movement, improves the movement accuracy of the scraping plate 12, and avoids deviation or shaking during the scraping of the cell fluid.
[0046] The blanking groove 8 is embedded at the bottom end of the culture dish 1, and a through connection is provided between the bottom opening 15 and the blanking groove 8. A through hole is provided at the upper end of the blanking groove 8, and the through hole penetrates the outer surfaces of the upper and lower ends of the scratch frame 2.
[0047] The sealing between the sealing ring 9 at the bottom of the scratch frame 2 and the culture dish 1, and the connection between the bottom opening 15 and the blanking groove 8 ensure the accurate collection of the cell fluid, prevent the leakage of the cell fluid during the experiment, ensure the collection and reuse of the experimental materials, and reduce the experimental cost.
[0048] Scale lines are arranged at the upper end of the scratch frame 2, and a pressing plate is provided at the upper end of the scraping plate 12.
[0049] The scale lines at the upper end of the scratch frame 2 and the pressing plate at the upper end of the scraping plate 12. The scale lines provide a reference for the accurate scratch position, and the pressing plate facilitates the operator to apply a stable pressure, improving the reliability of the experimental results.
[0050] When this application is in use: When a cell scratch experiment needs to be carried out, first place the scratch frame 2 into the culture dish 1. The clamping block 4 under the linkage handle 3 is clamped into the corresponding card slot 5 outside the culture dish 1, and through the action of the gravity block in the clamping block 4 on the linkage block 6, the telescopic spring 7 is compressed to ensure the stability of the scratch frame 2. Then inject the cell fluid into the scratch frame 2 so that the cell fluid fills the central position of the scratch frame 2. According to the scale lines on the scratch frame 2, press down the scraping plate 12. At this time, the telescopic rod 13 and the linkage spring 14 contract, and a part of the cell fluid is scraped by driving the scraping plate 12 through the pressing plate. The corresponding scraped cell fluid flows into the blanking groove 8 through the bottom opening 15. For the collection of this part of the cell fluid, a pipette is inserted through the through hole to collect the cell fluid collected in the blanking groove 8. After that, the culture dish 1 with the scratched cell fluid is placed under a microscope for observation. This process does not affect the cell scratch experiment and can comprehensively collect the scratched cell fluid at the same time.
[0051] The above embodiments are only used to illustrate the present utility model and not to limit the technical solutions described by the present utility model. Although this specification has described the present utility model in detail with reference to the above respective embodiments, the present utility model is not limited to the above specific implementation manners. Therefore, any modification or equivalent replacement to the present utility model; and all technical solutions and their improvements that do not depart from the spirit and scope of the utility model are all covered within the scope of the claims of the present utility model.
Claims
1. A special culture dish for cell scratch assay, comprising a culture dish (1), characterized in that, The inner end of the culture dish (1) is provided with a scratch frame (2). Both ends of the scratch frame (2) are fixed with linkage handles (3). The lower end of the linkage handle (3) is fixed with a clamping block (4). The outer end of the clamping block (4) is provided with a clamping groove (5). The inner end of the clamping groove (5) is provided with a linkage block (6) and a telescopic spring (7). The inner end of the scratch frame (2) is provided with a sliding groove (10). The inner end of the sliding groove (10) is provided with a linkage rod (11). The inner end of the linkage rod (11) is provided with a scraping plate (12). Both ends of the scraping plate (12) are provided with telescopic rods (13) and linkage springs (14). An embedded groove (16) and an embedded block (17) are arranged between the scraping plate (12) and the linkage rod (11). The lower end of the scratch frame (2) is provided with a sealing ring (9). A bottom opening (15) is arranged between the scratch frame (2) and the culture dish (1). The outer end of the bottom opening (15) is provided with a feeding groove (8).
2. The cell scratch test special culture dish according to claim 1, wherein, The clamping groove (5) wraps around the outer end of the clamping block (4). The linkage block (6) moves up and down along the clamping groove (5). The lower end of the linkage block (6) is movably connected to the telescopic spring (7).
3. The cell scratch test special culture dish according to claim 1, characterized in that, A gravity block is arranged in the clamping block (4). The sliding groove (10) is embedded and installed at the inner end of the scratch frame (2). The linkage rod (11) slides left and right along the inner part of the sliding groove (10). A through groove is arranged in the center of the linkage rod (11). The scraping plate (12) moves up and down along the through groove.
4. The cell scratch test special culture dish according to claim 1, wherein, Both ends of the telescopic rod (13) are respectively fixed to the upper end of the scraping plate (12) and the upper end of the linkage rod (11). The inner end of the telescopic rod (13) is movably connected to the linkage spring (14).
5. The cell scratch test special culture dish according to claim 1, characterized in that The embedded groove (16) is embedded in the inner side of the narrow edge of the scraping plate (12). The embedded block (17) is fixed to the inner end of the narrow edge of the through groove. The bottom of the scratch frame (2) is fixedly connected to the sealing ring (9).
6. The cell scratch test special culture dish according to claim 1, wherein The feeding groove (8) is embedded at the bottom end of the culture dish (1). The bottom opening (15) is connected through with the feeding groove (8). A through hole is arranged at the upper end of the feeding groove (8). The through hole penetrates through the outer surfaces of the upper and lower ends of the scratch frame (2).
7. The cell scratch test special culture dish according to claim 1, wherein, Scale lines are arranged at the upper end of the scratch frame (2).
8. The cell scratch test special culture dish according to claim 1, wherein, A pressing plate is arranged at the upper end of the scraping plate (12).