Sample detection apparatus

Abstract

The utility model provides sample detection equipment. The sample detection equipment comprises a body and one or more sample detection units arranged on the body, each sample detection unit comprises a detection chamber, and at least part of the area of the detection chamber optically penetrates through the two opposite sides of the body to form an optical window; the sample adding hole is used for communicating the detection chamber with the atmosphere; the exhaust hole is used for communicating the detection chamber with the atmosphere; and a reagent layer attached to a part or all of the inner wall surface of the detection chamber. According to the utility model, the reagent layer is attached to the inner wall surface of the detection chamber, so that accurate detection and analysis can be realized in a shorter time, the problems of long time consumption and inaccurate detection result in the whole detection process in the prior art are solved, and the detection and analysis efficiency and accuracy of a liquid sample are improved.

Description

Technical Field

[0001] This specification relates to the field of biomedical technologies, and particularly to a sample detection device. Background Art

[0002] Performing detection and analysis after staining or reacting a liquid sample with a reagent is a commonly used experimental detection method in fields such as life sciences, chemistry, medicine, and environmental science. Placing the reagent in a solid form in advance at specific positions of a sample detection device (which can also be called a sample detection chip), then allowing the sample to enter the sample detection device to react with the pre-placed reagent, and then performing detection, has advantages such as being convenient for carrying and storage, and having a low overall cost. It is a cutting-edge, advanced technology with great prospects for calibration, batch production, and industrialization.

[0003] However, the dissolution and diffusion of solid reagents require a process and time. After dissolution, it is also necessary to uniformly contact and fully react with the detection target (such as cells) in the sample. Otherwise, it will cause deviation in the detection results. Therefore, how to quickly dissolve, mix, and fully react with the sample has become the key technology for sample detection devices with pre-placed solid reagents. Summary of the Utility Model

[0004] To solve the above technical problems, the purpose of the present utility model is to provide a sample detection device.

[0005] To achieve the above purpose, an embodiment provides a sample detection device. The sample detection device includes a main body and one or more sample detection units provided on the main body. It is characterized in that the sample detection unit includes:

[0006] A detection chamber, at least part of the area of which optically penetrates the opposite sides of the main body to form an optical window;

[0007] A sample addition hole, which connects the detection chamber with the atmosphere;

[0008] An exhaust hole, which connects the detection chamber with the atmosphere; and

[0009] A reagent layer, which is attached to a part or all of the inner wall surface of the detection chamber.

[0010] As a further improved embodiment, the reagent layer is at least attached to the inner wall surface between the sample addition hole and the optical window.

[0011] As a further improved embodiment, the reagent layer is uniformly attached to a part or all of the inner wall surface of the detection chamber;

[0012] Alternatively, the reagent layer gradually decreases along the sample flow direction on the inner wall surface of the detection chamber.

[0013] In an embodiment of further improvement, the inner wall surface of the detection chamber includes a pair of opposite walls that are arranged along the sample flow direction;

[0014] The reagent layer is attached to one or both of the pair of walls.

[0015] In an embodiment of further improvement, the body includes a first functional sheet and a second functional sheet, and the first functional sheet and the second functional sheet are stacked and hermetically joined along a first direction;

[0016] The detection chamber is formed between the first functional sheet and the second functional sheet;

[0017] The pair of walls is perpendicular to the first direction, and the two are respectively located on the first functional sheet and the second functional sheet;

[0018] The optical window optically penetrates the first functional sheet and the second functional sheet along the first direction.

[0019] In an embodiment of further improvement, the sample addition hole and / or the exhaust hole penetrate the first functional sheet to communicate with the detection chamber; the reagent layer is attached to the second functional sheet;

[0020] The pore walls of the sample addition hole and the exhaust hole are not provided with a reagent layer, and the surface of the second functional sheet corresponding to the sample addition hole and the exhaust hole is attached or not attached with a reagent layer.

[0021] In an embodiment of further improvement, the height of the detection chamber in the first direction is 0.02 mm to 2 mm.

[0022] In an embodiment of further improvement, the sample addition hole and the exhaust hole are respectively located at opposite ends of the detection chamber.

[0023] In an embodiment of further improvement, the number of the optical windows is set to one or more, and at least one of the optical windows is located in the middle of the detection chamber in the sample flow direction and / or in the middle of the detection chamber in the direction perpendicular to the sample flow direction.

[0024] In an embodiment of further improvement, the detection chamber includes a plurality of optical windows;

[0025] The plurality of optical windows are located in the middle of the detection chamber along the sample flow direction and are evenly spaced perpendicular to the sample flow direction; and / or, the plurality of optical windows are evenly spaced along the sample flow direction.

[0026] Compared with the prior art, the beneficial effects of the present utility model are as follows: By attaching a reagent layer to the inner wall surface of the detection chamber, during the process of the sample entering and filling the detection chamber, the reagent in the reagent layer starts to dissolve (that is, the flow of the sample and the dissolution of the reagent occur simultaneously), and the reagent dissolved into the sample can be more quickly mixed in the detection chamber, and fully contact and react with the sample, so as to achieve accurate detection and analysis in a shorter time, solve the problems of long detection time and inaccurate detection results in the prior art, and improve the detection and analysis efficiency and accuracy of liquid samples. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 is a three-dimensional structural diagram of a sample detection device according to an embodiment of the present utility model;

[0028] Figure 2 is a three-dimensional perspective view of a sample detection device according to an embodiment of the present utility model;

[0029] Figure 3 is an exploded structural diagram of a sample detection device according to an embodiment of the present utility model;

[0030] Figure 4 is Figure 1 the sectional view along line A-A in

[0031] Figure 5 is an exploded structural diagram of a sample detection device according to a variant embodiment of the present utility model;

[0032] Figure 6 is a front view of a sample detection device according to an embodiment of the present utility model;

[0033] Figure 7 is a front view of a sample detection device according to another embodiment of the present utility model;

[0034] Figure 8 is a front view of a sample detection device according to still another embodiment of the present utility model;

[0035] Figure 9 is a front view of a sample detection device according to yet another embodiment of the present utility model. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0036] The present utility model will be described in detail below in conjunction with the specific embodiments shown in the drawings. However, these embodiments do not limit the present utility model, and any structural, method, or functional transformation made by those of ordinary skill in the art based on these embodiments is included in the protection scope of the present utility model.

[0037] Obviously, the accompanying drawings in the following description are only some examples or embodiments of this specification. For those of ordinary skill in the art, without creative efforts, this specification can also be applied to other similar scenarios based on these drawings. Unless obvious from the context or otherwise stated, the same reference numerals in the figures represent the same structure or operation.

[0038] In the various diagrams of the present utility model, for the convenience of illustration, the dimensions of certain structures or parts are exaggerated relative to other structures or parts. Therefore, it is only used to illustrate the basic structure of the subject matter of the present utility model.

[0039] It should be understood that the "system", "device", "unit" and / or "module" used herein is a way to distinguish different components, elements, parts, portions or assemblies at different levels. However, if other words can achieve the same purpose, the said words can be replaced by other expressions.

[0040] Flowcharts are used in this specification to illustrate the operations performed by the systems according to the embodiments of this specification. It should be understood that the operations before or after do not necessarily need to be executed precisely in sequence. On the contrary, the steps can be processed in reverse order or simultaneously. At the same time, other operations can also be added to these processes, or one or several steps can be removed from these processes.

[0041] Refer Figures 1 to 6 , an embodiment of the present utility model provides a sample detection device 100.

[0042] The sample detection device 100 can be used for cell culture, cell counting, concentration determination, imaging analysis or other determinations. In some embodiments, the sample detection device 100 can be designed to perform determinations for oncology, immuno-oncology, virology, cell therapy, cell line development, regenerative medicine (stem cell research), brewing science and renewable energy. It can be used to analyze cell lines (such as NCI-60 cancer cells), primary cells (PBMC, spleen cells, leukapheresis, apheresis, thymocytes), stem cells, platelets, red blood cells, yeasts, and algae, CHO cells, etc. In some embodiments, the sample detection device 100 can be used to perform determinations such as cell growth and proliferation, viability, cell size changes caused by activation, transduction efficiency, apoptosis, autophagy, cell cycle, senescence, ROS, mitochondrial potential and health, surface marker population analysis, intracellular staining population analysis, etc.

[0043] As Figure 1 and Figure 2 shown, the sample detection device 100 includes a main body 10 and one or more sample detection units 20 provided on the main body 10.

[0044] The main body 10 can be a plate-like structure provided with the sample detection units 20.

[0045] In some embodiments, the main body 10 can be a rectangular or square plate-like structure. In other embodiments, the main body 10 can also be a structure of other shapes such as circular, triangular, hexagonal, etc., which is not limited in this specification.

[0046] Each sample detection unit 20 can be used to process a sample for detection and analysis.

[0047] In the figure, 5 sample detection units 20 are exemplified side by side on the main body 10. Of course, in variant embodiments, there can be only one sample detection unit 20 on the main body 10; there can also be other numbers of sample detection units 20 on the main body 10, such as 2, 4, 6, 12, 24, 96, and the arrangement of these sample detection and analysis units 20 on the main body 10 can be side by side or in an array arrangement.

[0048] Furthermore, the sample detection unit 20 includes a detection chamber 30, a sample injection hole 40, and an exhaust hole 50.

[0049] The detection chamber 30 is formed on the main body 10, and the sample introduced by the sample injection hole 40 can enter the detection chamber 30.

[0050] In some embodiments, the detection chamber 30 can be a hollow chamber formed on the main body 10.

[0051] Specifically, referring to Figure 3 , the main body 10 includes a first functional sheet 11 and a second functional sheet 12, and the first functional sheet 11 and the second functional sheet 12 are stacked along a first direction. In the figure, the first direction is the thickness direction H of the main body 10. The detection chamber 30 is formed between the first functional sheet 11 and the second functional sheet 12 and is surrounded by the two.

[0052] For example, the first functional sheet 11 is provided with a groove region recessed along the thickness direction H, and the second functional sheet 12 is an equal-thickness plate. When the first functional sheet 11 and the second functional sheet 12 are stacked and assembled together, the groove region forms the detection chamber 30. In variant embodiments, it can also be that the first functional sheet 11 is an equal-thickness plate and the second functional sheet 12 is provided with a groove region recessed along the thickness direction H, and this groove region forms the detection chamber 30; or, it can be that both the first functional sheet 11 and the second functional sheet 12 are provided with groove regions recessed in the thickness direction H, and the groove regions of the two together form the detection chamber 30.

[0053] In addition, the stacking arrangement relationship of the first functional piece 11 and the second functional piece 12 in the first direction may specifically be that one surface of the first functional piece 11 in the first direction is attached to one surface of the second functional piece 12 in the first direction as shown in the figure, or the second functional piece 12 may be embedded into the groove area of the first functional piece 11.

[0054] At least part of the detection chamber 30 can optically penetrate through opposite sides of the main body 10. That is to say, at the at least part of the area, after a light beam penetrates through the main body 10 and the at least part of the area from one opposite side of the main body 10, it exits to the opposite side of the main body 10. In this way, the sample at the at least part of the area can be observed from outside the main body 10.

[0055] For example, when the main body 10 is implemented as a plate structure, at least part of the detection chamber 30 can optically penetrate through opposite sides in the thickness direction H of the main body 10. In this way, with the optical detection device introduced later, the sample at the at least part of the area is observed from at least one of the opposite sides in the thickness direction H of the main body 10.

[0056] The at least part of the area constitutes the optical window 31.

[0057] In one embodiment, a partial area of the detection chamber 30 constitutes the optical window 31, while the other areas except this are set to be unable to optically penetrate through opposite sides of the main body 10. In this way, the sample can only be observed at the position of the optical window 31.

[0058] In some variant embodiments, the entire area of the detection chamber 30 constitutes the optical window 31. In this way, the sample can be observed at any position in the detection chamber 30.

[0059] The optical window 31 can be constructed by the light-transmitting setting of the main body 10 itself.

[0060] Specifically, for example, in some embodiments, the area on the main body 10 corresponding to the optical window 31 is set as an optically transparent structure; and the areas on the main body 10 corresponding to other areas outside the optical window 31 are set as non-optically transparent structures, such as structures with a frosted layer, a non-light-transmitting coating or a non-light-transmitting film on the surface.

[0061] The sample injection hole 40 is provided on the main body 10, which connects the detection chamber 30 with the atmosphere to introduce a sample into the detection chamber 30 via the sample injection hole 40.

[0062] In some embodiments, the sample injection hole 40 can be a groove or a blind hole on the main body 10.

[0063] For example, the sample injection hole 40 may be recessed and extend along the thickness direction H of the main body 10 on the surface of the main body 10. More specifically, as Figure 3 shown, the sample injection hole 40 may penetrate the first functional sheet 11 along the thickness direction H to communicate with the detection chamber 30. Of course, it may also be changed to that the sample injection hole 40 may penetrate the second functional sheet 12 along the thickness direction H to communicate with the detection chamber 30.

[0064] In some embodiments, the cross-section of the sample injection hole 40 may be set to be circular, semi-circular, elliptical, square, triangular or other shapes. Here, the cross-section is perpendicular to the recessed extension direction of the sample injection hole 40, for example, perpendicular to the thickness direction H.

[0065] The exhaust hole 50 is provided on the main body 10, which communicates the detection chamber 30 with the atmosphere, so that the gas in the detection chamber 30 can be discharged through the exhaust hole 50 when the sample enters the detection chamber 30.

[0066] In some embodiments, the exhaust hole 50 may be a groove or a blind hole on the main body 10.

[0067] For example, the exhaust hole 50 may be recessed and extend along the thickness direction H of the main body 10 on the surface of the main body 10. More specifically, as Figure 3 shown, the exhaust hole 50 may penetrate the first functional sheet 11 along the thickness direction H to communicate with the detection chamber 30. Of course, it may also be changed to that the exhaust hole 50 may penetrate the second functional sheet 12 along the thickness direction H to communicate with the detection chamber 30.

[0068] In some embodiments, the cross-section of the exhaust hole 50 may be set to be circular, semi-circular, elliptical, square, triangular or other shapes. Here, the cross-section is perpendicular to the recessed extension direction of the exhaust hole 50, for example, perpendicular to the thickness direction H.

[0069] In the present utility model, the sample detection unit 20 further includes a reagent layer 60, so as to stain or perform other reactions on the sample through the reagent layer 60, and then detect and analyze the reacted sample.

[0070] The reagent layer 60 is attached to a part or all of the inner wall surface of the detection chamber 30, that is, the reagent layer 60 is attached to at least part of the inner wall surface of the detection chamber 30.

[0071] Thus, by attaching the reagent layer 60 to the inner wall surface of the detection chamber 30, when the sample enters and fills the detection chamber 30, the reagent in the reagent layer 60 starts to dissolve (i.e., the flow of the sample and the dissolution of the reagent occur simultaneously), and the reagent dissolved into the sample can be more quickly mixed in the detection chamber 30, and fully contact and react with the sample, so as to achieve accurate detection and analysis in a shorter time, solve the problems of long time consumption and inaccurate detection results in the prior art, and improve the detection and analysis efficiency and accuracy of liquid samples.

[0072] In some embodiments, the reagent of the reagent layer 60 is selected from any one or several combinations of a staining agent, an antibody, a protein, but is not limited thereto.

[0073] The staining agent can make normal cells and apoptotic or damaged cells present different colors, so as to classify and detect the activity of cells in the sample. In some embodiments, the staining agent includes, but is not limited to, any one or several combinations of reagents such as trypan blue, acridine orange (AO), fluorescent dye PI (propidium iodide), SYTO9, etc.

[0074] The reagent layer 60 is a solid structure, and specifically, it can be formed by attaching a coating containing the reagent to the inner wall surface of the detection chamber 30 by any one of soaking, wafer flowing, spraying, microarray, coating, and then drying and fixing to form the solid structure of the reagent layer 60. In this way, by setting the solid structure of the reagent layer 60, the reagent layer 60 can be firmly attached to the inner wall surface of the detection chamber 30, and the dissolution speed can be reasonable and will not dissolve too quickly like a reagent layer in a liquid state, so as to facilitate the control of the dissolution speed, improve the efficiency and ensure faster mixing at the same time.

[0075] In some embodiments, the coating includes a reagent and a solvent.

[0076] The solvent can specifically be selected from any one or several combinations of pure water, phosphate buffered saline (PBS), Tris (N-Tris(hydroxymethyl)aminomethane) buffer, physiological saline, and the specific solvent component can be selected according to the specific composition of the reagent.

[0077] In some embodiments, the coating can be prepared through the following process: the solid powder / granule / sheet of the reagent is dissolved in the reagent, and then stirred evenly to obtain the coating.

[0078] In some embodiments, the coating includes other additives, such as MOPS (3-morpholinopropanesulfonic acid), dibenzocyclooctyne-polyethylene glycol-amine DBCO-PEG-NH2.

[0079] Among them, MOPS (3-morpholinopropanesulfonic acid) can enhance the adhesion firmness of the reagent layer 60 to prevent the reagent layer 60 in the dry state from peeling off from the inner wall surface of the detection chamber 30.

[0080] Dibenzocyclooctyne-polyethylene glycol-amine (DBCO-PEG-NH2) can enhance the wettability of the reagent layer 60 to increase the flow rate of the sample, shorten the sample flow duration t1, and facilitate the rapid mixing of the reagent, thereby improving the overall efficiency of the sample detection process.

[0081] In some embodiments, after the coating adheres to the inner wall surface of the detection chamber 30, the specific drying and fixing method and conditions are drying in a constant temperature and humidity chamber at 20°C and RH30% for 30 minutes. In this way, it can not only ensure that the coating is fully dried, but also prevent the reagent from being damaged and rendered ineffective.

[0082] The reagent layer 60 adheres to at least a part of the inner wall surface of the detection chamber 30, and it adheres to at least the inner wall surface between the sample loading hole 40 and the optical window 31. In this way, the sample flows through at least a part of the reagent layer 60 before reaching the optical window 31, which can further accelerate the mixing of the reagent in the detection chamber 30 and improve the overall efficiency of the sample detection process.

[0083] The inner wall surface of the detection chamber 30 includes a wall extending along the sample flow direction, and the reagent layer 60 is preferably adhered to the wall, which can more facilitate the rapid dissolution of the reagent layer 60.

[0084] In some embodiments, the cross-section of the detection chamber 30 perpendicular to the sample flow direction can be constructed as a rectangle as shown in the attached drawings, or can also be various shapes such as a circle or a sector.

[0085] As shown in the figure, the detection chamber 30 is approximately cuboid-shaped, and its inner wall surface includes a pair of first walls arranged opposite to each other and a pair of second walls arranged opposite to each other.

[0086] Among them, the pair of first walls extend along the sample flow direction and are opposite to each other in the thickness direction H, and the pair of second walls extend along the sample flow direction and are perpendicular to the first walls.

[0087] The sample loading hole 40 and the exhaust hole 50 are respectively located at opposite ends of the detection chamber 30, and both penetrate through the first functional sheet 11 in the thickness direction H and are connected to the detection chamber 30.

[0088] The reagent layer 60 adheres to any one or a combination of several walls of the first wall and the second wall.

[0089] Furthermore, the reagent layer 60 is attached to a partial area or the entire area of any wall. That is, for example, when the reagent layer 60 is attached to one of the first walls, it may be that only a partial area of the first wall is attached with the reagent layer 60 while the remaining area of the wall is not attached with the reagent layer 60, or the entire area of the first wall may be attached with the reagent layer 60.

[0090] As shown in the illustrated embodiment, the pair of first walls are perpendicular to the thickness direction H.

[0091] One of the first walls 301 is formed on the first functional sheet 11. For the convenience of distinction and description, it is referred to as the upper wall 301; the other first wall 302 is formed on the second functional sheet 12. For the convenience of distinction and description, it is referred to as the lower wall 302.

[0092] In some embodiments, the reagent layer 60 is preferably attached to either or both of the upper wall 301 and the lower wall 302. In this way, based on the area and position of the upper wall 301 and the lower wall 302 (for example, extending along the sample flow direction), it is more conducive to the rapid dissolution of the reagent layer 60 to be completed.

[0093] For example, in one embodiment, the reagent layer 60 can be attached to both the upper wall 301 and the lower wall 302; for another example, in another embodiment, as Figure 3 and Figure 4 shown, the reagent layer 60 can be attached only to the upper wall 301; for another example, in another embodiment, as Figure 5 shown, the reagent layer 60 can be attached only to the lower wall 302.

[0094] Among them, compared with the upper wall 301, it is more preferable to attach the reagent layer 60 to the lower wall 302. In this way, in an application scenario where there are detection targets (such as cells) that are prone to sedimentation in the sample, preferably attaching the reagent layer 60 to the lower wall 302 can, while achieving the rapid dissolution of the reagent layer 60, facilitate the rapid contact and incubation between the reagent and the detection target, thereby further improving the detection result and detection efficiency.

[0095] Especially in some embodiments, the detection chamber 30 is a slit cavity formed between the upper wall 301 and the lower wall 302, and the height between the upper wall 301 and the lower wall 302 is narrow, that is, the height in the thickness direction H is small. In this way, preferably attaching the reagent layer 60 to the upper wall 301 and / or the lower wall 302 is more conducive to the sample contacting the reagent layer 60 as fully as possible during the flow process, thereby accelerating the dissolution of the reagent in the reagent layer 60, improving the mixing rate and reaction rate, and thus further improving the detection analysis efficiency and accuracy of the liquid sample as a whole.

[0096] In some embodiments, the height of the detection chamber 30 in the thickness direction H is approximately 0.02 mm to 2 mm. Correspondingly, the distance between the upper wall 301 and the lower wall 302 is approximately 0.02 mm to 2 mm. Of course, this is not limiting.

[0097] In the embodiments introduced above Figure 5 of the reagent layer 60 may be attached only to the lower wall 302. In specific implementation, it may be:

[0098] Before the second functional sheet 12 and the first functional sheet 11 are assembled together, the position of the lower wall 302 is determined in advance on a surface 122 of the second functional sheet 12 facing the first functional sheet 11;

[0099] Then, through a masking technique, a masking plate is shielded on the surface 122, and the masking plate exposes the position of the lower wall 302;

[0100] After that, the reagent layer 60 is attached to the position of the lower wall 302 by means such as spraying or brushing;

[0101] Finally, the masking plate is removed, and the attachment of the reagent layer 60 to the lower wall 302 can be achieved.

[0102] Among them, the "pre-determining the position of the lower wall 302 on a surface 122 of the second functional sheet 12 facing the first functional sheet 11" can be specifically confirmed by a positioning structure between the first functional sheet 11 and the second functional sheet 12. This positioning structure includes, for example, a convex post 101 on the first functional sheet 11 and a positioning hole 102 on the second functional sheet 12. Based on the insertion fit of the convex post 101 and the positioning hole 102, the position of the lower wall 302 corresponding to the position of the upper wall 301 can be determined on the surface 122 of the second functional sheet 12.

[0103] In addition, in Figure 5 it is shown that the reagent layer 60 is attached to the lower wall 302. On this basis, in further variant embodiments, a second reagent layer may also be additionally attached to the position P1 facing the sample injection hole 40 and the position P2 facing the exhaust hole 50 on the surface 122 of the second functional sheet 12. The second reagent layer has the same composition as the reagent layer 60. Of course, either or both of the position P1 and the position P2 may not be attached with a reagent layer.

[0104] That is to say, in further variant embodiments, either or both of the position P1 and the position P2 may be additionally coated or not coated with a reagent.

[0105] As described above, the detection chamber 30 is generally rectangular parallelepiped-shaped. In some variant embodiments, the detection chamber 30 may also be provided in a curved shape along the sample flow direction.

[0106] For the curved detection chamber 30 described above, the sample injection hole 40 and the exhaust hole 50 are respectively located at opposite ends of the detection chamber 30. The sample injection hole 40 communicates with the detection chamber 30 at one end, and the exhaust hole 50 communicates with the detection chamber 30 at the other end.

[0107] Among them, the cuboid detection chamber 30 shown in the drawings, or the curved detection chamber 30 described above, can be set to have a constant width in the width direction W.

[0108] Furthermore, in some embodiments, the reagent layer 60 is uniformly attached to part or all of the inner wall surface of the detection chamber 30. That is, whether the entire area of the inner wall surface of the detection chamber 30 is attached with the reagent layer 60, or only a partial area of the inner wall surface of the detection chamber 30 is attached with the reagent layer 60, the reagent layer 60 is uniform in these areas.

[0109] For example, the reagent layer 60 can be attached in equal amounts at each position along the sample flow direction in the detection chamber 30. In this way, at any two different positions along the sample flow direction, the attachment amount of the reagent layer 60 is the same, which is beneficial to improving the mixing rate.

[0110] In some embodiments, the reagent layer 60 gradually decreases along the sample flow direction on the inner wall surface of the detection chamber 30.

[0111] For example, along the sample flow direction, the attachment of the reagent layer 60 in the area relatively close to the sample injection hole 40 is more than that in the area relatively far from the sample injection hole 40. In this way, when the sample flows, the reagent in the reagent layer 60 dissolves and is carried by the sample towards the end where the exhaust hole 50 is located. Even so, the mixing rate can be further improved, thereby shortening the time-consuming of the entire sample detection.

[0112] Preferably, the reagent layer 60 decreases in a stepped arithmetic progression along the sample flow direction on the inner wall surface of the detection chamber 30.

[0113] Moreover, in the case of a stepped arithmetic progression decrease, the reagent layer 60 is uniformly attached to all or part of the inner wall surface of each stepped area of the detection chamber 30 along the sample flow direction.

[0114] Specifically, it can be divided into two stepped decreases. For example, the first half of the detection chamber 30 along the sample flow direction constitutes the first step, and the second half along the sample flow direction constitutes the second step. The reagent layer 60 is uniformly attached to all or part of the inner wall surface corresponding to the first step, and to all or part of the inner wall surface corresponding to the second step, and the total attachment amount of the reagent layer 60 on all or part of the inner wall surface corresponding to the first step is greater than the total attachment amount on all or part of the inner wall surface corresponding to the second step.

[0115] Specifically, it can be further divided into three stepped decreases. For example, the first 1 / 3 section of the detection chamber 30 along the sample flow direction constitutes the first step, the middle 1 / 3 section along the sample flow direction constitutes the second step, and the last 1 / 3 section along the sample flow direction constitutes the third step. The reagent layer 60 is uniformly attached to all or part of the inner wall surfaces corresponding to each step, and the total amount of attachment of the reagent layer 60 on all or part of the inner wall surfaces corresponding to each stepped section is: the first step > the second step > the third step.

[0116] Specifically, it can be further divided into four stepped or more stepped forms.

[0117] In addition, each step can be evenly divided along the sample flow direction. For example, the areas of the inner wall surfaces occupied by each step are the same; or, it can also be divided non-uniformly. For example, the areas of the inner wall surfaces occupied by each step are the same.

[0118] The number of optical windows 31 is set to one, two, or more.

[0119] Refer to Figures 4 to 6 , in some embodiments, the number of optical windows 31 is set to one, which is located in the middle of the detection chamber 30 along the sample flow direction. In this way, through the setting of the optical window 31, the sample detection can be completed in the shortest possible time and the high accuracy of the detection result can be ensured.

[0120] More preferably, the optical window 31 is located in the middle of the width direction W of the detection chamber 30.

[0121] The width direction W is perpendicular to the sample flow direction and the thickness direction H. In other words, the width direction W, the sample flow direction, and the thickness direction H are perpendicular to each other in pairs.

[0122] In some embodiments, the number of optical windows 31 is set to multiple, such as 2, 3, 4, 6, 9,....

[0123] In some embodiments, refer to Figure 7 , multiple optical windows 31 are located in the middle of the detection chamber 30 along the sample flow direction and are evenly spaced perpendicular to the sample flow direction, that is, evenly spaced along the width direction W.

[0124] In Figure 7 it is exemplified as 3 optical windows 31, one of which is located in the middle of the width direction W of the detection chamber 30, and the other two are distributed on both sides of the width direction W.

[0125] In some embodiments, refer to Figure 8 , multiple optical windows 31 are evenly spaced along the sample flow direction.

[0126] In Figure 8In the example, there are three optical windows 31. One of them is located in the middle of the detection chamber 30 along the sample flow direction, another is located near the sample injection hole 40, and the other is located near the exhaust hole 50.

[0127] In some embodiments, referring Figure 9 , multiple optical windows 31 are evenly spaced along the sample flow direction, and multiple optical windows 31 are located in the middle of the detection chamber 30 along the sample flow direction and are evenly spaced perpendicular to the sample flow direction.

[0128] In Figure 9 the example, there are nine optical windows 31. Three of them are located in the middle of the detection chamber 30 along the sample flow direction and are evenly spaced along the width direction W; another three are located near the sample injection hole 40 and are evenly spaced along the width direction W; and the other three are located near the exhaust hole 50 and are evenly spaced along the width direction W.

[0129] Preferably, at least three optical windows 31 are evenly spaced along the sample flow direction, and one of the optical windows 31 is located in the middle of the sample flow direction of the detection chamber 30.

[0130] When there are two or more optical windows 31, during the sample detection process, by observing the sample through these optical windows 31 respectively and then performing data analysis (for example, taking the average value of the observation results of each optical window 31), more accurate detection and analysis results can be obtained.

[0131] In some embodiments, the sample detection device 100 may further include a seal, and the seal is used to seal either or both of the sample injection hole 40 and the exhaust hole 50.

[0132] For example, during the sample detection process, after the detection chamber 30 is filled with the sample, the sample injection hole 40 and / or the exhaust hole 50 can be closed by the seal. At this time, the sample detection device 100 can be shaken or oscillated to shorten the time for reagent dissolution and mixing.

[0133] In some embodiments, the seal includes a sealing plug.

[0134] In some embodiments, the sealing plug includes a pull rod and a plug head. The plug head is detachably embedded in the sample injection hole 40 and / or the exhaust hole 50 to block the communication between the sample injection hole 40 and / or the exhaust hole 50 and the atmosphere. The pull rod can protrude from the sample injection hole 40 and / or the exhaust hole 50, facilitating the user to pull out the sealing plug from the sample injection hole 40 and / or the exhaust hole 50 by pulling the pull rod, so as to communicate the sample injection hole 40 and / or the exhaust hole 50 with the atmosphere.

[0135] In some embodiments, the seal includes a sealing film.

[0136] In some embodiments, the sealing film can be sealed at the injection hole 40 and / or the exhaust hole 50 to block the injection hole 40 and / or the exhaust hole 50 from being connected to the atmosphere. When it is necessary to release the seal, the injection hole 40 and / or the exhaust hole 50 can be connected to the atmosphere by tearing or puncturing the sealing film.

[0137] In some embodiments, the sealing film may be a thin film fixed to the injection hole 40 and / or the exhaust hole 50 by injection molding or adhesion.

[0138] In some embodiments, the seal comprises a sealing cap.

[0139] In some embodiments, the sealing cover is adapted to the injection hole 40 and / or the exhaust hole 50, and the sealing cover is detachably covered on the injection hole 40 and / or the exhaust hole 50 to block the injection hole 40 and / or the exhaust hole 50 from being connected to the atmosphere.

[0140] In some embodiments, the sealing cover may be mated with the injection hole 40 and / or the exhaust hole 50 via threads.

[0141] In some embodiments, the sealing cover can be engaged with the injection hole 40 and / or the exhaust hole 50 by snapping.

[0142] In some embodiments, the sample detection device 100 may also include a pressure-applying device for applying negative pressure to the exhaust hole 50, thereby increasing the power for the sample reagent to flow from the injection hole 40 to the detection chamber 30, allowing the sample reagent to flow quickly to the detection chamber 30 and improving operating efficiency.

[0143] In some embodiments, the pressure-applying device includes but is not limited to a syringe, a pipette, a peristaltic pump, a vacuum pump, and the like.

[0144] Further, as mentioned above, the body 10 may have a plurality of sample detection units 20. Different sample detection units 20 may detect different samples, or different sample detection units 20 may detect the same sample under different conditions, or different sample detection units 20 may detect the same sample under different reagents.

[0145] An application of the sample detection device according to one embodiment of the utility model is introduced below, for example, a method for applying it to sample detection: adding a sample into the detection chamber 30 through the sample addition hole 40 until the sample fills the detection chamber 30; using an optical detection device known in the art to observe the sample through the optical window 31 of the detection chamber 30.

[0146] In summary, the beneficial effects of an embodiment of the present utility model are at least as follows: By attaching a reagent layer 60 to the inner wall surface of the detection chamber 30, when the sample enters and fills the detection chamber 30, the reagent in the reagent layer 60 starts to dissolve (that is, the flow of the sample and the dissolution of the reagent occur simultaneously), and the reagent dissolved into the sample can be more quickly mixed in the detection chamber 30 and fully contact and react with the sample, so as to achieve accurate detection and analysis in a shorter time (for example, when applied to sample detection, sample observation can be carried out in a very short time after the sample is added to the detection chamber 30, and the obtained results are accurate), solving the problems of long detection time and inaccurate detection results in the prior art, and improving the detection and analysis efficiency and accuracy of liquid samples.

[0147] It should be understood that although this specification is described according to embodiments, not every embodiment only contains an independent technical solution. This narrative manner of the specification is only for clarity. Those skilled in the art should regard the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

[0148] The series of detailed descriptions listed above are only specific descriptions of the feasible embodiments of the present utility model, and are not intended to limit the protection scope of the present utility model. Any equivalent embodiments or changes made without departing from the technical spirit of the present utility model should be included in the protection scope of the present utility model.

Claims

1. A sample detection device, comprising a main body and one or more sample detection units provided on the main body, characterized in that, The sample detection unit includes: a detection chamber, at least part of the area of which optically penetrates the opposite sides of the main body to form an optical window; a sample addition hole, which connects the detection chamber to the atmosphere; an exhaust hole, which connects the detection chamber to the atmosphere; and a reagent layer, which is attached to part or all of the inner wall surface of the detection chamber.

2. The sample detection device according to claim 1, wherein The reagent layer is at least attached to the inner wall surface between the sample addition hole and the optical window.

3. The sample detection device according to claim 1, characterized in that, The reagent layer is uniformly attached to part or all of the inner wall surface of the detection chamber; alternatively, the reagent layer gradually decreases along the sample flow direction on the inner wall surface of the detection chamber.

4. The sample detection device according to claim 2, characterized in that, The inner wall surface of the detection chamber includes a pair of oppositely arranged walls, and the pair of walls extend along the sample flow direction; The reagent layer is attached to one or both of the pair of walls.

5. The sample detection device according to claim 4, characterized in that The main body includes a first functional sheet and a second functional sheet, and the first functional sheet and the second functional sheet are stacked and sealed in a first direction; The detection chamber is formed between the first functional sheet and the second functional sheet; The pair of walls are perpendicular to the first direction, and are respectively located on the first functional sheet and the second functional sheet; The optical window optically penetrates the first functional sheet and the second functional sheet in the first direction.

6. The sample detection device according to claim 5, characterized in that, The sample addition hole and / or the exhaust hole penetrate the first functional sheet to communicate with the detection chamber; The reagent layer is attached to the second functional sheet and is set as a staining agent layer; The inner walls of the sample addition hole and the exhaust hole are not provided with a reagent layer, and the surface of the second functional sheet corresponding to the sample addition hole and the exhaust hole is attached or not attached with a reagent layer.

7. The sample detection device according to claim 5, wherein, The height of the detection chamber in the first direction is 0.02 mm to 2 mm.

8. The sample detection device according to claim 1, characterized in that, The sample addition hole and the exhaust hole are respectively located at opposite ends of the detection chamber.

9. The sample detection device according to claim 1, wherein, The number of the optical windows is set to one or more, and at least one of the optical windows is located in the middle of the detection chamber in the sample flow direction and / or in the middle of the detection chamber in the direction perpendicular to the sample flow direction.

10. The sample detection device according to claim 1, characterized in that, The detection chamber includes a plurality of optical windows; The plurality of optical windows are located in the middle of the detection chamber along the sample flow direction and are evenly spaced perpendicular to the sample flow direction; and / or, the plurality of optical windows are evenly spaced along the sample flow direction.

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