Dual-optical-path microscopic projection optical system

Abstract

The utility model discloses a double-light-path microscopic projection optical system, which has the following beneficial effects: (1) a light combination assembly, a fluorescence module and an objective lens assembly are shared, a bright field imaging light path and a fluorescence imaging light path are designed, the two light paths are of a symmetrical structure, and through the adjustment of the objective lens assembly and a microscopic shooting assembly, the imaging quality is improved; the optical paths of the two optical paths to the respective cameras are consistent, so that a bright field and fluorescence synchronous focusing imaging function is realized; (2) compared with an existing single-light-path microscopic system, large-view-field shooting is added, the speed is high, image splicing is avoided, image restoration is real, and images can be rapidly positioned; and (3) the DMD projection module is added, so that additional functions such as photoelectric tweezers and SIM super-resolution imaging can be realized, and the application range is wide.

Description

Technical Field

[0001] The utility model relates to the technical field of microscopic imaging, and in particular to a dual-path microscopic projection optical system. Background Art

[0002] At present, microscopes have been widely used in the field of life sciences and related industrial fields. However, traditional microscopic systems have disadvantages such as a small field of view and single function, which are mainly manifested in the following two aspects:

[0003] (1) Small field of view: For traditional microscopes, the shooting range is small. In general, to achieve large-scale microscopic imaging, the stage needs to move in the XY direction, and then the microscopic objective lens takes step-by-step shots, and finally image stitching is performed. This method takes a certain amount of time due to the mechanical movement of the stage, and the image stitching algorithm also requires time for calculation, resulting in a long time consumption for large image shooting.

[0004] (2) No additional functions: Traditional microscopes do not have additional DMD projection modules, etc., and cannot meet the requirements of special scenarios such as optical tweezers and SIM imaging.

[0005] Therefore, how to design a microscopic projection optical system with a large field of view, high efficiency, and the ability to achieve special functions such as optical tweezers and SIM imaging is a technical problem to be solved. Summary of the Utility Model

[0006] Based on this, it is necessary to provide a dual-path microscopic projection optical system with a large field of view, high efficiency, and the ability to achieve special functions such as optical tweezers and SIM imaging for existing problems.

[0007] The embodiment of the present application provides a dual-path microscopic projection optical system, including a bright-field imaging light path and a fluorescence imaging light path, and the two light paths share a beam combining module, a fluorescence module, and an objective lens assembly;

[0008] The bright-field imaging light path includes: a first light source emits a first light beam, the first light beam enters the DMD module, the first light beam after being processed by the DMD enters the beam combining component, the first light beam is reflected by the beam combining component and then enters the fluorescence module, is transmitted through the fluorescence module to the objective lens assembly, the first light beam collected by the objective lens assembly enters the microscopic shooting module and then enters the first reflecting mirror, and after reflection, enters the imaging lens for convergence, and the converged first light beam enters the first camera module;

[0009] The fluorescence imaging light path includes: a second light source emits fluorescence, the fluorescence light beam enters the fluorescence module and is reflected by it and then enters the objective lens assembly, and then enters the microscopic shooting module, is collected by the objective lens assembly, passes through the fluorescence module, the beam combining module, and the lens and then enters the second reflecting mirror, and is reflected by the second reflecting mirror to the second camera module.

[0010] Preferably, the objective lens assembly includes at least two objective lens units with different magnifications.

[0011] Preferably, the beam combining module includes a guide rail, a first motor, and multiple beam channels; the first motor is used to drive the beam channels to move on the guide rail to achieve the switching of different beam channels.

[0012] Preferably, the fluorescence module includes a guide rail, a second motor, multiple fluorescence channels, and a bright field illumination channel; the second motor is used to drive the multiple fluorescence channels and a bright field illumination channel to move on the guide rail to achieve the switching of multiple different fluorescence channels.

[0013] The beam combining module includes a first motor and multiple lenses provided with different beam channels; the first motor is used for the switching of multiple different beam channels.

[0014] Preferably, the fluorescence module includes a second motor, multiple lenses provided with fluorescence channels, and a bright field illumination channel; the second motor is used for the switching of multiple different fluorescence channels.

[0015] Preferably, the dual - optical - path microscopic projection optical system further includes a third motor, a fourth motor, and a fifth motor for controlling the objective lens assembly;

[0016] The third motor is used to control the change of the X - axis position of the objective lens assembly; the fourth motor is used to control the change of the Y - axis position of the objective lens assembly; the fifth motor is used to control the change of the Z - axis position of the objective lens assembly.

[0017] Preferably, the bright field imaging optical path and the fluorescence imaging optical path are coaxial.

[0018] Compared with the prior art, the technical solution disclosed by the present utility model has the following beneficial effects:

[0019] (1) The present invention shares a light combining component, a fluorescence module, and an objective lens assembly, designs a bright field imaging optical path and a fluorescence imaging optical path, both of which are symmetric structures. Through the adjustment of the objective lens assembly and the microscopic shooting component, the optical paths of the two optical paths reaching their respective cameras are made consistent, thereby realizing the function of synchronous focusing imaging of bright field and fluorescence;

[0020] (2) Compared with the existing single - optical - path microscopic system, it has increased large - field - of - view shooting, fast speed, no image stitching, real image restoration, and can quickly locate the image;

[0021] (3) It has added a DMD projection module, which can be used to realize additional functions such as optical tweezers and SIM super - resolution imaging, and has a wide range of applications. Description of the Drawings

[0022] The exemplary embodiments of the present utility model can be more fully understood by referring to the following accompanying drawings. The accompanying drawings are used to provide a further understanding of the embodiments of the present application, and constitute a part of the specification. Together with the embodiments of the present application, they are used to explain the present utility model, and do not constitute a limitation to the present utility model. In the accompanying drawings, the same reference numerals generally represent the same components or steps.

[0023] Figure 1 FIG. 4 is a schematic structural diagram of a dual - optical - path microscopic projection optical system provided according to an exemplary embodiment of the present application;

[0024] Figure 2 FIG. 8 is a schematic structural diagram of a beam - combining module provided according to an exemplary embodiment of the present application;

[0025] Figure 3 FIG. 12 shows a schematic structural diagram of a fluorescence module provided according to an exemplary embodiment of the present application;

[0026] Figure 4 FIG. 16 shows a top - view structural diagram of an objective lens assembly provided according to an exemplary embodiment of the present application;

[0027] Figure 5 FIG. 20 shows a side - view structural diagram of an objective lens assembly provided according to an exemplary embodiment of the present application.

[0028] Reference Numerals

[0029] 101 - First camera module, 102 - Second camera module, 103 - Second reflector, 104 - First reflector, 105 - Lens, 106 - Beam - combining module, 107 - First light source, 108 - DMD module, 109 - Fluorescence module, 110 - Second light source, 111 - Objective lens assembly, 112 - Microscopic shooting module, 113 - Imaging lens, 201 - First motor, 202 - Beam channel, 301 - Second motor, 302 - Bright - field illumination channel, 303 - Fluorescence channel, 401 - Third motor, 402 - Fourth motor, 403 - Fifth motor. Detailed Embodiments

[0030] The exemplary embodiments of the present disclosure will be described in more detail below with reference to the accompanying drawings. Although the exemplary embodiments of the present disclosure are shown in the accompanying drawings, it should be understood that the present disclosure can be implemented in various forms and should not be limited by the embodiments set forth herein. On the contrary, these embodiments are provided so that the present disclosure can be more thoroughly understood and the scope of the present disclosure can be fully conveyed to those skilled in the art.

[0031] In the description of the present utility model, it should be noted that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present utility model. In addition, the terms "first", "second", "third" are only used for descriptive purposes and cannot be construed as indicating or implying relative importance.

[0032] In the description of the present utility model, it should be noted that unless otherwise clearly specified and defined, the terms "installed", "connected", "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.

[0033] In addition, the technical features involved in different embodiments of the present utility model described below can be combined with each other as long as they do not conflict with each other.

[0034] Embodiment 1

[0035] The embodiment of the present application provides a dual-path microscopic projection optical system, including a bright-field imaging optical path and a fluorescence imaging optical path, which will be described below with reference to the drawings.

[0036] Referring to Figure 1 , which shows a dual-path microscopic projection optical system provided by some embodiments of the present application. As Figure 1 shown, the system includes:

[0037] Among them, the bright-field imaging optical path includes: a first light source 107 emits a first light beam, the first light beam enters the DMD module 108, the first light beam after being processed by the DMD module 108 enters the beam combining component 106, the first light beam is reflected by the beam combining component 106 and then enters the fluorescence module 109, is transmitted through the fluorescence module 109 to the objective lens component 111, the first light beam collected by the objective lens component 111 enters the microscopic photographing module 112 and is incident on the first reflecting mirror 104, is reflected and then enters the imaging lens 113 for convergence, and the converged first light beam enters the first camera module 101.

[0038] In this embodiment, the first light source 107 is the illumination light source of the projection part. Its light source can be monochromatic light or polychromatic light. When it is polychromatic light, the system controls the wavelength switching of the light source. Its light source can be an LED light source or a laser light source, or a combination of the two. Specifically, the brightness of the light source is controlled by the system.

[0039] Among them, the fluorescence imaging optical path includes: the second light source 110 emits fluorescence, and the fluorescence beam enters the fluorescence module 109, is reflected and then enters the objective lens assembly 111, and then enters the microscopic shooting module 112. After being collected by the objective lens assembly 111, it passes through the fluorescence module 109, the beam combining module 106 and the lens 105 and then enters the second reflector 103, and is reflected by the second reflector 103 to the second camera module 102.

[0040] In this embodiment, the second light source 110 is the illumination part of the fluorescence, and includes a wide-spectrum light source or multiple monochromatic light sources.

[0041] Specifically, the two camera modules respectively shoot the same position on the central axis of the microscopic shooting module 112. Among them, the optical path of the second camera module 102 is equipped with an objective lens 111 with a large magnification and is responsible for shooting the microscopic part; while the first camera module 101 is equipped with an optical system with a relatively small resolution and is responsible for overall shooting, that is, the picture taken by the camera 101 is within the reflection range of the second camera module 102. Specifically, the bright-field imaging optical path and the fluorescence imaging optical path are coaxially arranged.

[0042] In this embodiment, 105 is a lens, which can also be set as a lens group. The optical path formed by it and the objective lens assembly 111 is used for microscopic function; 108 is a DMD projection system, and its main function is to project a pattern below the objective lens.

[0043] Refer to Figure 2 , the beam combining component 106 includes a first motor 201 and multiple lenses 202 provided with different beam channels; among them, the first motor 201 is used for switching multiple different beam channels. There is a guide rail below the multiple channels, and the multiple channels move along the guide rail driven by the motor to realize the switching of multiple channels.

[0044] Refer to Figure 3 , the fluorescence module 109 includes a second motor 301, multiple lenses 303 provided with fluorescence channels and a bright-field illumination channel 302; the second motor is used for switching multiple different fluorescence channels. There is a guide rail below the multiple channels, and the multiple channels move along the guide rail driven by the motor to realize the switching of multiple channels.

[0045] Refer to Figure 4-5 , the system further includes a third motor 401, a fourth motor 402 and a fifth motor 405 for controlling the objective lens assembly 111.

[0046] The third motor 401 is used to control the change of the X-axis position of the objective lens assembly 111; the fourth motor 402 is used to control the change of the Y-axis position of the objective lens assembly 111; the fifth motor 403 is used to control the change of the Z-axis position of the objective lens assembly 111. Specifically, the control of the objective lens is achieved by controlling the turntable, which is controlled by the motor. The control of the third motor 401 and the fourth motor 402 is to make the shooting surfaces of the two cameras the same plane; the fifth motor 403 is used to adjust the Z-axis position of the turntable; specifically, the objective lens assembly 111 includes a plurality of objective lens units with different magnifications, and the objective lens assembly 111 can adjust the angle along the circumference as needed to achieve the switching of different objective lenses.

[0047] In this embodiment, through the coordinated control of the system, automatic focusing of the illumination bright field light path and the fluorescence imaging light path can be achieved. According to the shooting position of the first camera module 101, by controlling the movement of the XY axis of the shooting plane, the second camera module 102 is used to perform high-magnification shooting. The fluorescence channel gear and the beam combining channel gear can be adjusted to switch the magnification of the objective lens assembly 111 used by the second camera 102, and image instructions can be sent to the DMD projection module 108 in the projection light path to project the corresponding image, ultimately realizing functions such as photoelectric tweezers and SIM super-resolution imaging.

[0048] Specifically, when realizing the photoelectric tweezers function, it is necessary to add appropriate periodic voltage to the upper and lower plates of the photographed glass slide, and form an electric field within the range of the beam irradiation by irradiating the light beam to different positions, so that the material is repelled or attracted by the electric field to achieve the manipulation of the material. When realizing the SIM super-resolution imaging function, the illumination field is used to project three stripe patterns with an angle interval of 120° to achieve the improvement of the optical shooting limit resolution.

[0049] Compared with the prior art, the technical solution disclosed in the utility model has the following beneficial effects:

[0050] (1) The present invention uses a common light combining component, a fluorescence module and an objective lens component to design a bright field imaging optical path and a fluorescence imaging optical path. Both optical paths are symmetrical in structure. By adjusting the objective lens component and the microscopic shooting component, the optical paths of the two optical paths to their respective cameras are consistent, thereby realizing the function of bright field and fluorescence synchronous focusing imaging;

[0051] (2) Compared with the existing single-light path microscope system, it has a large field of view, fast speed, no image stitching, true image restoration, and can quickly locate the image;

[0052] (3) The addition of a DMD projection module can realize additional functions such as photoelectric tweezers and SIM super-resolution imaging, with a wide range of applications.

[0053] It can be understood that the same or similar parts in the above embodiments can be referred to each other. For the content not detailed in some embodiments, reference can be made to the same or similar content in other embodiments.

[0054] It should be noted that in the description of the present utility model, the terms "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or implying relative importance. In addition, in the description of the present utility model, unless otherwise specified, the meaning of "a plurality of" refers to at least two.

[0055] In the description of this specification, the description referring to terms such as "one embodiment", "some embodiments", "example", "specific example", or "some examples" means that the specific features, structures, materials, or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present utility model. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described can be combined in a suitable manner in any one or more embodiments or examples.

[0056] Although the embodiments of the present utility model have been shown and described above, it can be understood that the above embodiments are exemplary and cannot be construed as limiting the present utility model. Those of ordinary skill in the art can make changes, modifications, substitutions, and variations to the above embodiments within the scope of the present utility model.

Claims

1. A dual-light-path microscopic projection optical system, characterized in that, It includes a bright-field imaging optical path and a fluorescence imaging optical path, and the two optical paths share a beam combining module, a fluorescence module, and an objective lens assembly; The bright-field imaging optical path includes: a first light source emits a first light beam, the first light beam enters the DMD module, the first light beam after being processed by the DMD module enters the beam combining module, the first light beam is reflected by the beam combining module and then enters the fluorescence module, is transmitted through the fluorescence module to the objective lens assembly, the first light beam collected by the objective lens assembly enters the microscopic shooting module and then enters the first reflector, and after being reflected, enters the imaging lens for convergence, and the converged first light beam enters the first camera module; The fluorescence imaging optical path includes: a second light source emits fluorescence, the fluorescence light beam enters the fluorescence module and is reflected by it and then enters the objective lens assembly, and then enters the microscopic shooting module, is collected by the objective lens assembly, passes through the fluorescence module, the beam combining module, and the lens and then enters the second reflector, and is reflected by the second reflector to the second camera module.

2. The dual-path microscopic projection optical system according to claim 1, wherein, The objective lens assembly includes at least two objective lens units with different magnifications.

3. A dual-path microscopic projection optical system according to claim 1, wherein, The beam combining module includes a guide rail, a first motor, and multiple beam channels; the first motor is used to drive the beam channels to move on the guide rail to achieve the switching of different beam channels.

4. A dual-path microscopic projection optical system according to claim 1, characterized in that, The fluorescence module includes a guide rail, a second motor, multiple fluorescence channels, and a bright-field illumination channel; the second motor is used to drive the multiple fluorescence channels and a bright-field illumination channel to move on the guide rail to achieve the switching of multiple different fluorescence channels.

5. A dual-path microscopic projection optical system according to claim 1, wherein The dual-optical-path microscopic projection optical system further includes a third motor, a fourth motor, and a fifth motor for controlling the objective lens assembly; The third motor is used to control the change in the X-axis position of the objective lens assembly; The fourth motor is used to control the change in the Y-axis position of the objective lens assembly; The fifth motor is used to control the change in the Z-axis position of the objective lens assembly.

6. The dual-path microscopic projection optical system according to claim 1, characterized in that, The bright-field imaging optical path and the fluorescence imaging optical path are coaxial.

Images