Centrifugal device for exosome extraction

Abstract

The utility model relates to the technical field of exosome extraction, and discloses a centrifugal device for exosome extraction, which comprises an outer shell, a rotating rod is rotatably connected in the outer shell, the top of the rotating rod is fixedly connected with a side gear, the top of the side gear is fixedly connected with a connecting clamping plate, and the connecting clamping plate is fixedly connected with the rotating rod. A side plate is fixedly connected to the side wall of the outer shell, a connecting ring is rotatably connected to the inner wall of the side plate, a test tube rack is fixedly connected to the inner wall of the connecting ring, a connecting jacking column is fixedly connected to the outer wall of the test tube rack, an inner connecting plate is fixedly connected to the inner wall of the connecting jacking column, and a limiting groove is formed in the surface of the inner connecting plate. According to the device, a side gear structure is arranged, a main gear drives a side gear to rotate, a clamping block structure is arranged at the bottom of a connecting column, a clamping block can be connected with a connecting clamping plate arranged at the top of the side gear, and the position of a sliding block is changed from the interior of a limiting groove by rotating after upward pressing.

Description

Technical Field

[0001] The utility model relates to the technical field of exosome extraction, in particular to a centrifugal device for exosome extraction. Background Art

[0002] Exosomes are nanoscale vesicles secreted by cells, which have rich bioactive substances and important physiological functions. Due to their potential biomedical application value, the extraction and analysis of exosomes have become a research hotspot. A high-speed centrifuge is one of the commonly used devices for exosome extraction. Through high-speed centrifugation, exosomes can be effectively separated from cell culture supernatants or biological fluids.

[0003] Currently, the main method of the device is to place the test tube on the test tube rack, then connect the test tube rack to the motor rotation structure at the bottom, and then realize the centrifugal motion of the exosomes inside the test tube through the rotation of the motor, so as to realize the extraction and collection of exosomes and the working mode of centrifugal extraction.

[0004] The current device has certain deficiencies. First of all, the existing device can only perform centrifugation work on one test tube at a time. Although there are multi-test tube common centrifugation works, according to different reagent requirements, the centrifugation time of the test tubes is different, and it is not possible to better realize the centrifugal extraction work. Moreover, the existing device cannot better change the centrifugation method, the effect is too single, and the efficiency is not high enough. Summary of the Utility Model

[0005] In order to make up for the above deficiencies, the utility model provides a centrifugal device for exosome extraction, aiming to improve the problems that the centrifugation time cannot be controlled and the centrifugation method is too single in the existing technology.

[0006] In order to achieve the above purpose, the utility model adopts the following technical scheme: A centrifugal device for exosome extraction, including an outer housing, a rotating rod is rotatably connected inside the outer housing, a side gear is fixedly connected to the top of the rotating rod, a connecting clamping plate is fixedly connected to the top of the side gear, a side plate is fixedly connected to the side wall of the outer housing, a connecting ring is rotatably connected to the inner wall of the side plate, a test tube rack is fixedly connected to the inner wall of the connecting ring, a connecting top column is fixedly connected to the outer wall of the test tube rack, an inner connecting plate is fixedly connected to the inner wall of the connecting top column, a limiting groove is formed on the surface of the inner connecting plate, a connecting column is slidably connected to the inner wall of the limiting groove, a sliding block is fixedly connected to the side wall of the connecting column, a spring is fixedly connected to the inner top wall of the inner connecting plate, a pressing plate is fixedly connected to the bottom end of the spring, a clamping block is fixedly connected to the bottom of the connecting column, and a driving component is arranged at the bottom of the outer housing for driving and connecting;

[0007] As a further description of the above technical solution: The bottom of the pressing plate is fixedly connected to the top of the connecting column. A chute is provided at the top of the side plate. A square block is fixedly connected to the outer wall of the test tube rack. A connecting bar is fixedly connected to the bottom of the square block. A pulley is rotatably connected to the inner wall of the connecting bar. The pulley is slidably connected to the chute by mutual cooperation;

[0008] As a further description of the above technical solution: A fixed block is fixedly connected to the outer wall of the test tube rack. A bolt is threadedly connected to the inner wall of the fixed block. One end of the bolt is threadedly connected to a clamping block. Two groups of test tube racks are fixedly connected to one group of fixed blocks, and the clamping blocks are symmetrically arranged;

[0009] As a further description of the above technical solution: The driving assembly includes a motor. The output end of the motor is fixedly connected to a main gear. The top of the main gear is fixedly connected to a rotating disk through a rotating shaft. The top of the rotating disk is fixedly connected to a connecting frame. The top of the connecting frame is fixedly connected to the test tube rack;

[0010] As a further description of the above technical solution: The outer wall of the main gear is meshed with the side gear. The number of side gears is four groups. All four groups of side gears are meshed with the main gear. The motor is fixedly connected to the bottom of the outer housing;

[0011] As a further description of the above technical solution: The main gear and the side gear are rotatably connected relative to the outer housing. The main gear is arranged at the middle position of the inner bottom wall of the outer housing;

[0012] As a further description of the above technical solution: A box door is rotatably connected to the front side of the outer housing. A viewing window is installed on the front side of the box door. A handle is fixedly connected to the front side of the box door;

[0013] As a further description of the above technical solution: Bottom columns are fixedly connected to the bottom of the outer housing. The bottom columns are evenly arranged around the bottom of the outer housing.

[0014] The present utility model has the following beneficial effects:

[0015] 1. In the present utility model, through the set side gear structure, the main gear drives the side gear to rotate. A clamping block structure is provided at the bottom of the connecting column. The clamping block can be connected to the connecting clamping plate provided at the top of the side gear. After pressing upward and then rotating, the position of the sliding block is changed from the inside of the limiting groove. Then, the connecting column will be pressed downward according to the action of the spring. Then, the clamping block is connected to the connecting clamping plate, and then the rotation of the top structure is realized, and the rotation of this test tube rack is realized, which can better control the number of rotations of the test tube rack, so as to adjust according to requirements, which is convenient and fast.

[0016] 2. In the present utility model, the main gear structure can better realize different rotation modes of the test tube rack at the top of the main gear during centrifugation. It adds a revolution centrifugation mode, which can meet the extraction requirements for different reagents or different methods, providing more options and a wider application range. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 A three-dimensional schematic diagram of a centrifugal device for exosome extraction proposed by the present utility model;

[0018] Figure 2 A structural schematic diagram of the outer casing of a centrifugal device for exosome extraction proposed by the present utility model;

[0019] Figure 3 A structural schematic diagram of the side plate of a centrifugal device for exosome extraction proposed by the present utility model;

[0020] Figure 4 A structural schematic diagram of the connecting column of a centrifugal device for exosome extraction proposed by the present utility model;

[0021] Figure 5 is Figure 4 An enlarged view of the structure at A in

[0022] Legend Explanation:

[0023] 1. Outer casing; 2. Box door; 3. Visual window; 4. Handle; 5. Bottom column; 6. Motor; 7. Main gear; 8. Rotating disk; 9. Connecting frame; 10. Connecting ring; 11. Side gear; 12. Rotating rod; 13. Connecting clamping plate; 14. Clamping block; 15. Connecting column; 16. Connecting top column; 17. Pressing plate; 18. Spring; 19. Test tube rack; 20. Fixed block; 21. Bolt; 22. Clamping block; 23. Side plate; 24. Square block; 25. Connecting bar; 26. Pulley; 27. Chute; 28. Limiting groove; 29. Sliding block; 30. Inner connecting plate. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0024] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described with reference to the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present utility model without creative efforts shall fall within the protection scope of the present utility model.

[0025] Refer to Figure 1 . Figure 3 . Figure 4 and Figure 5, an embodiment provided by the present utility model: a centrifugation device for exosome extraction, including an outer housing 1. The function of the outer housing 1 is to carry and support the entire device. A rotating rod 12 is rotatably connected inside the outer housing 1. The function of the rotating rod 12 is to connect and rotate the side gear 11. A side gear 11 is fixedly connected to the top of the rotating rod 12. The function of the side gear 11 is to better drive the test tube rack 19 at the top to achieve rotation, so as to better achieve the centrifugal motion. A connection card plate 13 is fixedly connected to the top of the side gear 11. The function of the connection card plate 13 is to connect the block at the bottom of the connection column 15. After connection, better rotation can be achieved, and the rotation adjustment of the test tube rack can be carried out according to the requirements of the centrifugal motion. A side plate 23 is fixedly connected to the side wall of the outer housing 1. The function of the side plate 23 is to achieve better rotation. A connection ring 10 is rotatably connected to the inner wall of the side plate 23. The function of the connection ring 10 is to ensure the stability of the test tube rack 19. A test tube rack 19 is fixedly connected to the inner wall of the connection ring 10. The function of the test tube rack 19 is to fix the test tubes and better achieve the centrifugal motion. A connection top column 16 is fixedly connected to the outer wall of the test tube rack 19. An inner connection plate 30 is fixedly connected to the inner wall of the connection top column 16. The inner connection plate 30 is arranged inside the top column 16, and an "L"-shaped limiting groove 28 is formed on the surface of the inner connection plate 30 to realize the rotation of the connection column 15. Thus, through the action of the spring 18, the block 14 structure at the bottom of the connection column 15 is connected to the connection card plate 13 structure, achieving the effect that the side gear 11 drives the top structure to rotate. A limiting groove 28 is formed on the surface of the inner connection plate 30. A connection column 15 is slidably connected to the inner wall of the limiting groove 28. A sliding block 29 is fixedly connected to the side wall of the connection column 15. A spring 18 is fixedly connected to the inner top wall of the inner connection plate 30. The bottom end of the spring 18 is fixedly connected to a pressing plate 17. The pressing plate 17 is connected to the spring to press the bottom connection column 15. A block 14 is fixedly connected to the bottom of the connection column 15, which cooperates with the limiting groove 28 to achieve the connection and fixation function. A driving component is arranged at the bottom of the outer housing 1, and the driving component is used for driving and connecting.

[0026] The bottom of the pressing plate 17 is fixedly connected to the top of the connection column 15. A chute 27 is formed at the top of the side plate 23. A square block 24 is fixedly connected to the outer wall of the test tube rack 19. A connection bar 25 is fixedly connected to the bottom of the square block 24. A pulley 26 is rotatably connected to the inner wall of the connection bar 25. The pulley 26 performs circular motion inside the chute 27 to achieve the supporting and rotating auxiliary functions of the test tube rack 19. The function of the connection bar 25 is to connect the pulley 26 to the square block 24, thereby connecting to the test tube rack 19. The pulley 26 and the chute 27 cooperate with each other to achieve sliding connection.

[0027] The outer wall of the test tube rack 19 is fixedly connected with a fixing block 20. The inner wall of the fixing block 20 is threadedly connected with a bolt 21. One end of the bolt 21 is threadedly connected with a clamping block 22. Two groups of test tube racks 19 are fixedly connected to a group of fixing blocks 20, and the clamping blocks 22 are symmetrically arranged to clamp the test tubes.

[0028] Refer to Figure 1 , the driving component includes a motor 6. The function of the motor 6 is to achieve the driving effect, thereby driving the entire structure to rotate. The output end of the motor 6 is fixedly connected with a main gear 7. The top of the main gear 7 is fixedly connected with a rotating disk 8 through a rotating shaft. The rotating disk 8 connects the test tube rack 19 structure at the top to achieve revolution centrifugation. The top of the rotating disk 8 is fixedly connected with a connecting frame 9. The top of the connecting frame 9 is fixedly connected with a test tube rack 19. The outer wall of the main gear 7 is meshed with a side gear 11 to drive the side gear 11 to rotate. The number of side gears 11 is four groups. All four groups of side gears 11 are meshed with the main gear 7. The motor 6 is fixedly connected to the bottom of the outer housing 1. The main gear 7 and the side gear 11 are rotatably connected relative to the outer housing 1. The main gear 7 is arranged at the middle position of the inner bottom wall of the outer housing 1 to achieve a stable effect.

[0029] Refer to Figure 2 , a box door 2 is rotatably connected to the front side of the outer housing 1 to achieve sealing. A viewing window 3 is installed on the front side of the box door 2 to observe the internal real-time changes. A handle 4 is fixedly connected to the front side of the box door 2. A bottom column 5 is fixedly connected to the bottom of the outer housing 1 to achieve a supporting effect. The bottom columns 5 are evenly arranged around the bottom of the outer housing 1.

[0030] Working principle: First, the exosomes to be centrifugally extracted are adjusted through the bolt 21 arranged on the outer wall of the test tube rack 19 and then placed inside the test tube and clamped by the clamping block 22. After clamping, press the rotating connecting column 15 inward. At this time, the sliding block 29 fixedly connected to the connecting column 15 will move inside the limiting groove 28 on the inner connecting plate 30, and then move from the short end to the long end, and then apply a downward force to the connecting column 15 through the action of the spring 18 and the pressing plate 17. Finally, the connection between the clamping block 14 and the connecting card plate 13 is achieved. Then start the motor 6. At this time, the main gear 7 arranged at the output end of the motor 6 will rotate, and then the side gear 11 meshed with it will also rotate, thereby driving the test tube rack structure connected to the top of the side gear 11 to rotate. The connection ring 10 is connected to the side plate 23 to achieve the smoothness of the test tube centrifugal movement. Select the fixed position of the test tube according to different centrifugation times and centrifugation requirements to achieve the extraction work of exosomes.

[0031] Finally, it should be noted that the above are only the preferred embodiments of the present invention and are not used to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A centrifugation device for exosome extraction, comprising an outer housing (1), characterized in that: Inside the outer shell (1), a rotating rod (12) is rotatably connected. At the top of the rotating rod (12), a side gear (11) is fixedly connected. At the top of the side gear (11), a connecting clamping plate (13) is fixedly connected. On the side wall of the outer shell (1), a side plate (23) is fixedly connected. Inside the inner wall of the side plate (23), a connecting ring (10) is rotatably connected. Inside the inner wall of the connecting ring (10), a test tube rack (19) is fixedly connected. On the outer wall of the test tube rack (19), a connecting top column (16) is fixedly connected. Inside the inner wall of the connecting top column (16), an inner connecting plate (30) is fixedly connected. On the surface of the inner connecting plate (30), a limiting groove (28) is formed. Inside the inner wall of the limiting groove (28), a connecting column (15) is slidably connected. On the side wall of the connecting column (15), a sliding block (29) is fixedly connected. On the inner top wall of the inner connecting plate (30), a spring (18) is fixedly connected. At the bottom end of the spring (18), a pressing plate (17) is fixedly connected. At the bottom of the connecting column (15), a clamping block (14) is fixedly connected. At the bottom of the outer shell (1), a driving assembly is provided, and the driving assembly is used for driving and connecting.

2. The centrifugal device for exosome extraction according to claim 1, wherein: The bottom of the pressing plate (17) is fixedly connected to the top of the connecting column (15). On the top of the side plate (23), a sliding groove (27) is formed. On the outer wall of the test tube rack (19), a square block (24) is fixedly connected. At the bottom of the square block (24), a connecting strip (25) is fixedly connected. Inside the inner wall of the connecting strip (25), a pulley (26) is rotatably connected. The pulley (26) is slidably connected with the sliding groove (27) in a matching manner.

3. The centrifugation device for exosome extraction according to claim 1, characterized in that: On the outer wall of the test tube rack (19), a fixing block (20) is fixedly connected. Inside the inner wall of the fixing block (20), a bolt (21) is threadedly connected. At one end of the bolt (21), a clamping block (22) is threadedly connected. Two groups of the test tube racks (19) are fixedly connected to one group of the fixing blocks (20), and the clamping blocks (22) are symmetrically arranged.

4. A centrifugation device for exosome extraction according to claim 1, characterized in that: The driving assembly includes a motor (6). At the output end of the motor (6), a main gear (7) is fixedly connected. At the top of the main gear (7), a rotating disk (8) is fixedly connected through a rotating shaft. At the top of the rotating disk (8), a connecting frame (9) is fixedly connected. At the top of the connecting frame (9), the test tube rack (19) is fixedly connected.

5. The centrifugal device for exosome extraction according to claim 4, wherein: On the outer wall of the main gear (7), the side gear (11) is meshed and connected. The number of the side gears (11) is four groups, and all four groups of the side gears (11) are meshed and connected with the main gear (7). The motor (6) is fixedly connected to the bottom of the outer shell (1).

6. The centrifugal device for exosome extraction according to claim 5, wherein: The main gear (7) and the side gear (11) are rotatably connected with respect to the outer shell (1), and the main gear (7) is arranged at the middle position of the inner bottom wall of the outer shell (1).

7. A centrifugal device for exosome extraction according to claim 1, characterized in that: On the front side of the outer shell (1), a box door (2) is rotatably connected. On the front side of the box door (2), a viewing window (3) is installed. On the front side of the box door (2), a handle (4) is fixedly connected.

8. The centrifugation device for exosome extraction according to claim 1, characterized in that: The bottom of the outer housing (1) is fixedly connected with bottom columns (5), and the bottom columns (5) are uniformly arranged around the bottom of the outer housing (1).

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