Automatic integrated equipment for repairing heavy metal contaminated soil

Abstract

The utility model provides automatic integrated equipment for repairing heavy metal contaminated soil. The automatic integrated equipment comprises a facultative anaerobic bacteria culture device, an aerobic bacteria culture box, a pollution pilot plant test box and a controller, the facultative anaerobic bacteria culture device comprises a facultative anaerobic bacteria culture box and an energy substance supply box; heavy metal contaminated soil is placed in the pollution pilot test box, the pollution pilot test box is connected with the facultative anaerobic bacteria incubator through a first header pipe, and a first delivery pump is arranged on the first header pipe; the pollution pilot test box is connected with the aerobic bacteria culture box through a second main pipe, and a second delivery pump is arranged on the second main pipe; the controller is connected with the first conveying pump and the second conveying pump and used for controlling opening or closing of the first conveying pump and / or the second conveying pump. The equipment provided by the utility model can automatically control the dosage of the conveyed bacterial liquid, and does not need to manually spray or put the microbial bacterial liquid, so that the labor cost is greatly reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of soil remediation, and particularly relates to an automatic integrated device for remediating heavy metal contaminated soil. Background Art

[0002] In the processes of non-ferrous metal mining, ore dressing and smelting, a large amount of solid waste such as tailings, smelting slag and various dusts and sludges will be left. If the solid waste is directly stacked in the open air, the solid waste will cause serious heavy metal pollution to the surrounding soil after being leached by rainwater.

[0003] The remediation technologies for heavy metal contaminated soil include physical measures, chemical agent addition processes and microbial remediation. Among them, the microbial remediation technology uses certain microorganisms in the polluted environment to carry out biological reduction, biomineralization, biosorption, bioaccumulation, etc. on heavy metals, reducing the mobility, high toxicity and bioavailability of heavy metals in solid waste or soil, which conforms to the economic, green and sustainable remediation concept.

[0004] However, in the actual application process of the microbial remediation technology, generally, microbial inoculants are manually sprayed or put into the heavy metal contaminated soil. Therefore, it is difficult to accurately control and remotely continuously dynamically monitor the dosage of the sprayed or put inoculants and the cultivation conditions of the microbial inoculants, and it is time-consuming, laborious, with high labor costs and poor operability. At the same time, due to the huge differences in the pollutant concentration, environmental conditions, etc. of the polluted soil in different regions, etc., it further leads to that it is very difficult to accurately control the relevant parameters in the traditional microbial spraying or putting, and the operability is relatively low. Summary of the Utility Model

[0005] To solve the above problems, the utility model provides an automatic integrated device for remediating heavy metal contaminated soil. The automatic integrated device includes: a facultative anaerobic bacteria culture device, an aerobic bacteria incubator, a pollution pilot box and a controller; the facultative anaerobic bacteria culture device includes a facultative anaerobic bacteria incubator and an energy substance supply box;

[0006] Heavy metal contaminated soil is placed in the pollution pilot box. The pollution pilot box is connected to the facultative anaerobic bacteria incubator through a first main pipe, and a first delivery pump is arranged on the first main pipe;

[0007] The pollution pilot box is connected to the aerobic bacteria incubator through a second main pipe, and a second delivery pump is arranged on the second main pipe;

[0008] The controller is respectively connected to the first delivery pump and the second delivery pump, and the controller is used to control the opening or closing of the first delivery pump and / or the second delivery pump.

[0009] Further, the pollution pilot box includes at least one cylindrical pipe, and the upper end of the cylindrical pipe is connected to the first main pipe;

[0010] A first discharge port is provided at the lower end of the facultative anaerobic bacteria incubator. One end of the first delivery pump is connected to the first discharge port, and the other end is connected to the first main pipe.

[0011] Further, the cylindrical pipe is vertically arranged inside the pollution pilot box and is buried in the heavy metal contaminated soil below;

[0012] At least one small hole is longitudinally distributed on the pipe wall of the cylindrical pipe; the bottom of the cylindrical pipe is sealed, and at least one layer of gauze is wrapped around the side.

[0013] Further, the pollution pilot box includes at least one spray pipe, the spray pipe is arranged at the top of the pollution pilot box, and the spray pipe is connected to the second main pipe;

[0014] A second discharge port is provided at the lower end of the aerobic bacteria incubator. One end of the second delivery pump is connected to the second discharge port, and the other end is connected to the second main pipe.

[0015] Further, the spray pipe 32 is arranged in a grid shape.

[0016] Further, a feed port is provided above the facultative anaerobic bacteria incubator; a third discharge port is provided at the lower end of the energy substance supply box, and the third discharge port is connected to the feed port above the facultative anaerobic bacteria incubator through a third main pipe; a third delivery pump is arranged on the third main pipe, and the third delivery pump is connected to the controller, and the controller is used to control the opening or closing of the third delivery pump; the facultative anaerobic bacteria cultivation device includes a circulation water tank, an inlet is provided above the circulation water tank, and a first water outlet is provided at the lower end;

[0017] A cube water tank is provided at the inner bottom of the pollution pilot box, and the heavy metal contaminated soil is placed on the cube water tank; a drain hole is provided at the top of the cube water tank, and a second water outlet is provided at the bottom, and the second water outlet is connected to the inlet through a water outlet pipe; a first valve is arranged on the water outlet pipe, and the first valve is connected to the controller, and the controller is used to control the opening or closing of the first valve;

[0018] The first water outlet is connected to the feed port through a fourth main pipe, a fourth circulation pump is arranged on the fourth main pipe, and the fourth circulation pump is connected to the controller, and the controller is used to control the opening or closing of the fourth circulation pump.

[0019] Furthermore, a first liquid level gauge is provided inside the cubic water tank. The first liquid level gauge is connected to the controller. The controller is configured to receive a first liquid level signal sent by the first liquid level gauge and control the opening or closing of the first valve according to the first liquid level signal;

[0020] A second liquid level gauge is provided inside the circulation water tank. The second liquid level gauge is connected to the controller. The controller is configured to receive a second liquid level signal sent by the second liquid level gauge and control the opening or closing of the fourth delivery pump according to the second liquid level signal.

[0021] Furthermore, a first chemical addition port is further provided at the upper end of the facultative anaerobe incubator. A second valve is provided on the first chemical addition port. The second valve is connected to the controller. The controller is configured to control the opening or closing of the second valve; a second chemical addition port is provided at the upper end of the aerobe incubator. A third valve is provided on the second chemical addition port. The third valve is connected to the controller. The controller is configured to control the opening or closing of the third valve;

[0022] Agitators are provided at the centers of the upper ends of the facultative anaerobe incubator, the energy substance supply tank, and the aerobe incubator; the agitator consists of a motor, a stirring shaft, and stirring blades. The motor is connected to the stirring blades through the stirring shaft. The motor is connected to the controller. The controller is configured to control the opening or closing of the motor.

[0023] Furthermore, an energy substance detector and a first pH meter are provided inside the facultative anaerobe incubator; both the energy substance detector and the first pH meter are connected to the controller; a second pH meter is provided inside the aerobe incubator; the second pH meter is connected to the controller;

[0024] The controller is configured to receive a detection signal sent by the energy substance detector and control the opening or closing of the third delivery pump according to the detection signal; the controller is configured to receive a pH signal sent by the first pH meter and / or the second pH meter and control the opening or closing of the second valve and / or the third valve according to the pH signal.

[0025] Furthermore, a first temperature sensor is provided inside the facultative anaerobe incubator, and a second temperature sensor is provided inside the aerobe incubator. The controller is respectively connected to the first temperature sensor and the second temperature sensor. The controller is configured to receive a temperature signal sent by the first temperature sensor and / or the second temperature sensor;

[0026] Both the facultative anaerobe incubator and the aerobic bacteria incubator are provided with electric heating devices at the bottom; the electric heating devices are connected to the controller, and the controller is used to control the opening or closing of the electric heating devices according to the temperature signal;

[0027] Heat preservation interlayers are arranged around both the facultative anaerobe incubator and the aerobic bacteria incubator.

[0028] Compared with the prior art, the present utility model has the following advantages:

[0029] The present utility model provides an automated integrated device for repairing heavy metal contaminated soil. The automated integrated device includes: a facultative anaerobe cultivation device, an aerobic bacteria incubator, a pollution pilot box, and a controller; the facultative anaerobe cultivation device includes a facultative anaerobe incubator and an energy substance supply box; heavy metal contaminated soil is placed in the pollution pilot box, and the pollution pilot box is connected to the facultative anaerobe incubator through a first main pipe, and a first delivery pump is arranged on the first main pipe; the pollution pilot box is connected to the aerobic bacteria incubator through a second main pipe, and a second delivery pump is arranged on the second main pipe; the controller is respectively connected to the first delivery pump and the second delivery pump, and the controller is used to control the opening or closing of the first delivery pump and / or the second delivery pump. The device provided by the present utility model can automatically control the dosage of the delivered bacterial liquid, without the need for manual spraying or putting the microbial bacterial liquid, greatly reducing the labor cost.

[0030] In the embodiment of the present utility model, the controller controls the opening or closing of the first delivery pump to remotely and precisely control whether to deliver the bacterial liquid containing facultative anaerobes to the pollution pilot box through the first main pipe; the controller controls the opening or closing of the second delivery pump to remotely and precisely control whether to deliver the bacterial liquid containing aerobic bacteria to the pollution pilot box through the second main pipe, thereby automatically controlling the dosage of the delivered bacterial liquid, without the need for manual spraying or putting the microbial bacterial liquid. In addition, the device provided by the present utility model can directly put the on-site cultivated microbial bacterial liquid into the heavy metal contaminated soil, avoiding the re-dissolution of heavy metals caused by untimely putting of the bacterial liquid. Brief Description of the Drawings

[0031] In order to more clearly illustrate the technical solutions of the present utility model, the following will be briefly introduced in conjunction with the drawings. Obviously, the drawings in the following description are only some embodiments of the present application. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0032] Figure 1 It is a schematic diagram of an automated integrated device for repairing heavy metal contaminated soil provided by the present utility model;

[0033] Figure 2This is the control diagram of the automated integrated device for repairing heavy metal contaminated soil provided by the present utility model. Detailed implementation manners

[0034] The embodiments of the present utility model will be described in detail below. The examples of the embodiments are shown in the accompanying drawings, where the same or similar reference numerals represent the same or similar elements or elements with the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present utility model and should not be construed as a limitation of the present utility model.

[0035] In the description of the present utility model, it should be understood that with respect to the orientation description, such as the upper, lower, front, rear, left, right, etc., the orientation or positional relationship indicated is based on the orientation or positional relationship shown in the accompanying drawings. It is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the present utility model.

[0036] In the description of the present utility model, the meaning of "several" is one or more, the meaning of "multiple" is two or more, "greater than", "less than", "exceeding", etc. are understood as not including the present number, and "above", "below", "within", etc. are understood as including the present number. If there is a description of "first" and "second", it is only for the purpose of distinguishing technical features and should not be construed as indicating or implying relative importance or implicitly indicating the quantity of the indicated technical features or the sequence relationship of the indicated technical features.

[0037] In the description of the present utility model, unless otherwise clearly defined, words such as "set", "installed", "connected", etc. should be understood in a broad sense, and those skilled in the art can reasonably determine the specific meaning of the above words in the present utility model in combination with the specific content of the technical solution.

[0038] In addition, the technical solutions between the various embodiments of the present utility model can be combined with each other, but it must be based on the fact that those skilled in the art can implement it. When the combination of technical solutions results in contradictions or cannot be implemented, it should be considered that such a combination of technical solutions does not exist and is not within the protection scope required by the present utility model.

[0039] The present utility model provides an automated integrated device for repairing heavy metal contaminated soil, such as Figure 1As shown in the figure, the automated integration device includes: a facultative anaerobic bacteria culture device 1, an aerobic bacteria incubator 2, a pollution pilot test box 3, and a controller 4; the facultative anaerobic bacteria culture device 1 includes a facultative anaerobic bacteria incubator 11 and an energy substance supply box 12; heavy metal contaminated soil is placed in the pollution pilot test box 3, and the pollution pilot test box 3 is connected to the facultative anaerobic bacteria incubator 11 through a first main pipe 51, and a first transfer pump 61 is provided on the first main pipe 51; the pollution pilot test box 3 is connected to the aerobic bacteria incubator 2 through a second main pipe 52, and a second transfer pump 62 is provided on the second main pipe 52; the controller 4 is respectively connected to the first transfer pump 61 and the second transfer pump 62, and the controller 4 is used to control the opening or closing of the first transfer pump 61 and / or the second transfer pump 62.

[0040] In the device provided by the embodiment of the present invention, continuous cultivation of facultative anaerobic bacteria is carried out in the facultative anaerobic bacteria incubator 11, and the bacterial liquid containing facultative anaerobic bacteria is transported to the pollution pilot test box 3 through the first main pipe 51; continuous cultivation of aerobic bacteria is carried out in the aerobic bacteria incubator 2, and the bacterial liquid containing aerobic bacteria is transported to the pollution pilot test box 3 through the second main pipe 52. The pollution pilot test box 3 uses the aerobic bacteria and / or facultative anaerobic bacteria in the bacterial liquid to carry out a pilot test on the heavy metal contaminated soil placed in the pollution pilot test box 3. By directly putting the microbe bacterial liquid cultured on-site into the heavy metal contaminated soil, the device provided by the present invention can avoid the re-dissolution of heavy metals caused by untimely delivery of the bacterial liquid.

[0041] Further, the facultative anaerobic bacteria are sulfate-reducing bacteria, and the aerobic bacteria are phosphorus-solubilizing bacteria; sulfate-reducing bacteria are cultured in the facultative anaerobic bacteria incubator 11 and the bacterial liquid containing sulfate-reducing bacteria is transported to the pollution pilot test box 3, and phosphorus-solubilizing bacteria are cultured in the aerobic bacteria incubator 2 and the bacterial liquid containing phosphorus-solubilizing bacteria is transported to the pollution pilot test box 3 at the same time. Therefore, the aerobic bacteria incubator 2 and the facultative anaerobic bacteria culture device 1 in this device continuously cultivate and provide phosphorus-solubilizing bacteria and sulfate-reducing bacteria to carry out combined remediation of phosphorus-solubilizing bacteria - sulfate-reducing bacteria on the heavy metal contaminated soil.

[0042] As Figure 2 shown, the controller 4 includes two power input terminals Y1 and Y2, a plurality of signal output terminals (Y3, Y4, Y5, Y6, Y7, Y8, Y9, Y10, Y11, Y12, Y13, Y14, Y15) and signal input terminals (Y16, Y17, Y18, Y19, Y20, Y21, Y22); the power input terminals Y1 and Y2 are respectively connected to the two poles of a storage battery or an external power supply, Figure 2 taking the case of power supply by a storage battery as an example.

[0043] The device provided by the embodiment of the present invention can automatically control the dosage of the transported bacterial liquid without manual spraying or putting the microbe bacterial liquid. Specifically, as Figure 2As shown in the figure, the first transfer pump 61 is connected to one of the signal output terminals Y3 of the controller 4 through a wire; the controller 4 controls the opening or closing of the first transfer pump 61 to accurately control whether to transfer the bacterial solution containing sulfate-reducing bacteria to the pollution pilot box 3. The second transfer pump 62 is connected to one of the signal output terminals Y4 of the controller 4 through a wire; the controller 4 controls the opening or closing of the second transfer pump 62 to accurately control whether to transfer the bacterial solution containing phosphate-solubilizing bacteria to the pollution pilot box 3.

[0044] In addition, the device provided by the embodiment of the present utility model can be programmed, remotely connected to a computer terminal, remotely monitored and operated for continuous transfer of the microbial bacterial solution, realizing refined management of soil remediation, and having the characteristics of high automation degree and low labor cost; specifically, the controller 4 includes a remote signal transmission module for remotely connecting to a PC or APP, facilitating remote operation by devices such as computers and mobile phones, so that the operator can still know the operation status of the device when not around the device, thereby realizing remote dynamic monitoring and operation.

[0045] In some embodiments of the present utility model, the pollution pilot box 3 includes at least one cylindrical pipe 31, and the upper end of the cylindrical pipe 31 is connected to the first main pipe 51;

[0046] A first discharge port 111 is provided at the lower end of the facultative anaerobic bacteria incubator 11, one end of the first transfer pump 61 is connected to the first discharge port 111, and the other end is connected to the first main pipe 51.

[0047] In this embodiment, continuous cultivation of the bacterial solution containing sulfate-reducing bacteria is carried out in the facultative anaerobic bacteria incubator 11, and the obtained bacterial solution containing sulfate-reducing bacteria flows out from the first discharge port 111. After the controller 4 controls the first transfer pump 61 to open, the flowing bacterial solution is poured into the cylindrical pipe 31 along the first main pipe 51, and the bacterial solution containing sulfate-reducing bacteria poured into the cylindrical pipe 31 is used for the remediation of soil in a deep anoxic environment.

[0048] Furthermore, the distance between adjacent cylindrical pipes 31 is 0.5 m 2 , the cylindrical pipe 31 is a hard PVC pipe, the nominal outer diameter of the cylindrical pipe 31 is 25 mm or 50 mm, and the pipe length of the cylindrical pipe 31 is 1.2 m.

[0049] In some embodiments of the present utility model, the cylindrical pipe 31 is vertically arranged inside the pollution pilot box 3 and is buried underground into the heavy metal contaminated soil;

[0050] At least one small hole 311 is longitudinally distributed on the pipe wall of the cylindrical pipe 31; the bottom of the cylindrical pipe 31 is sealed, and at least one layer of gauze 312 is wrapped around the side.

[0051] In this embodiment, the sulfate-reducing bacteria solution filled in the cylindrical tube 31 flows out from the small holes 311 longitudinally distributed on the tube wall and seeps into the surrounding soil. The sulfate-reducing bacteria in the bacteria solution reduce Fe 3+ / SO4 2- to Fe 2+ / H2S. The highly reducing Fe 2+ / H2S further reduces the highly toxic heavy metals in the soil to metal sulfide precipitates, so as to achieve the reduction and remediation of the soil in the deep anoxic environment.

[0052] Specifically, one end of the sealed cylindrical tube 31 is immersed in the heavy metal contaminated soil; the other end is completely open and connected to the first main pipe 51, which is the inlet for filling the bacteria solution.

[0053] Specifically, the aperture of the small hole 311 is 1 cm, and the interval between adjacent small holes 311 is 3 cm to 8 cm; the gauze wrapped around the side of the cylindrical tube 31 is used to prevent the heavy metal contaminated soil in the pollution pilot box 3 from entering the cylindrical tube through the small holes 311 and causing blockage inside the tube, thereby avoiding affecting the injection effect of the bacteria solution. Exemplarily, a small hole 311 is opened every 5 cm on the cylindrical tube 31, and 8 layers of cotton gauze are wrapped around the side of the cylindrical tube 31.

[0054] Furthermore, as Figure 1 shown, the first main pipe 51 is arranged in parallel with multiple cylindrical tubes 31, and a fourth valve 74 is provided at the connection of each cylindrical tube 31 and the first main pipe 51; as Figure 2 shown, the fourth valve 74 is connected to one of the signal output terminals Y10 of the controller 4 through a wire. The controller 4 controls the opening or closing of each fourth valve 74 to more accurately remotely control the amount of the sulfate-reducing bacteria solution injected into each cylindrical tube 31, so as to control the amount of the bacteria solution seeping into the heavy metal contaminated soil from the small holes 311 on the tube wall.

[0055] In some embodiments of the present utility model, the pollution pilot box 3 includes at least one spray pipe 32, the spray pipe 32 is arranged at the top of the pollution pilot box 3, and the spray pipe 32 is connected to the second main pipe 52;

[0056] A second discharge port 21 is provided at the lower end of the aerobic bacteria incubator 2. One end of the second delivery pump 62 is connected to the second discharge port 21, and the other end is connected to the second main pipe 52.

[0057] In this embodiment, continuous cultivation of phosphate-solubilizing bacteria solution is carried out in the aerobic bacteria incubator 2. The bacteria solution containing phosphate-solubilizing bacteria obtained from the cultivation flows out from the second discharge port 21. The controller controls the second delivery pump 62 to open, and the bacteria solution flows along the second main pipe 52 to multiple spray pipes 32. The multiple spray pipes 32 spray the bacteria solution containing phosphate-solubilizing bacteria onto the surface of the heavy metal contaminated soil. The bacteria solution contains phosphate-solubilizing enzymes secreted during the self-metabolism process of the phosphate-solubilizing bacteria. The phosphate-solubilizing enzymes can decompose the insoluble phosphorus-containing substances in the heavy metal contaminated soil into free phosphate complexes. The phosphate complexes undergo coprecipitation reactions with heavy metals in the surface soil to form stable salt minerals, thereby achieving surface solidification repair of the heavy metal contaminated soil.

[0058] In some embodiments of the present invention, the spray pipe 32 is arranged in a grid shape.

[0059] In this embodiment, the grid-shaped spray pipe 32 can more efficiently achieve surface mineralization repair of the heavy metal contaminated soil.

[0060] The equipment provided by the present invention combines the spraying of phosphate-solubilizing bacteria and the injection of sulfate-reducing bacteria by simultaneously arranging the spray pipe 32 and the cylindrical pipe 31 in the pollution pilot box 3. The combination of special bacteria and the equipment can most effectively repair heavy metal contaminated soil; specifically, the spraying of phosphate-solubilizing bacteria can solve the heavy metal pollution in solid waste or the soil surface layer, while the injection of sulfate-reducing bacteria can solve the heavy metal pollution in the deep anoxic environment, thereby achieving simultaneous repair of the surface oxygen-containing soil and the lower anoxic soil.

[0061] Regarding the dosage and duration of spraying and injection, no specific restrictions are made here. It can be adjusted and controlled by the controller 4 according to the actual microbial cultivation and soil repair conditions to achieve remote monitoring and operation of the spraying and injection of the microbial bacteria solution; for example, once a week, the controller 4 controls the fourth valve 74 to open to supplement the bacteria solution containing sulfate-reducing bacteria into the cylindrical pipe 31, and then the controller 4 closes the fourth valve 74. Once a week, the controller 4 controls the second delivery pump 62 to open to supplement the spraying of the bacteria solution containing phosphate-solubilizing bacteria onto the soil surface layer, and then the controller 4 closes the second delivery pump 62. The combined repair of uranium-contaminated soil by phosphate-solubilizing bacteria - sulfate-reducing bacteria is carried out with a 90-day repair cycle; for example, 0.1 L to 0.5 L of the bacteria solution containing sulfate-reducing bacteria is injected per square meter of heavy metal contaminated soil, and 0.1 L to 0.5 L of the bacteria solution containing phosphate-solubilizing bacteria is sprayed per square meter of heavy metal contaminated soil.

[0062] In some embodiments of the present utility model, a feed inlet 112 is provided above the facultative anaerobe incubator 11; a third discharge port 121 is provided at the lower end of the energy substance supply tank 12, and the third discharge port 121 is connected to the feed inlet 112 above the facultative anaerobe incubator 11 through a third main pipe 53; a third transfer pump 63 is provided on the third main pipe 53, and the third transfer pump 63 is connected to the controller 4, and the controller 4 is used to control the opening or closing of the third transfer pump 63;

[0063] The facultative anaerobe culturing device 1 includes a circulation water tank 13, an inlet 131 is provided above the circulation water tank 13, and a first water outlet 132 is provided at the lower end;

[0064] At the bottom inside the pollution pilot test tank 3, there is a cubic water tank 33, and the heavy metal contaminated soil is placed on the cubic water tank 33; a drain hole 331 is provided at the top of the cubic water tank 33, and a second water outlet 332 is provided at the bottom, and the second water outlet 332 is connected to the inlet 131 through a water outlet pipe 54; a first valve 71 is provided on the water outlet pipe 54, and the first valve 71 is connected to the controller 4, and the controller 4 is used to control the opening or closing of the first valve 71;

[0065] The first water outlet 132 and the feed inlet 112 are connected through a fourth main pipe 55, a fourth transfer pump 64 is provided on the fourth main pipe 55, and the fourth transfer pump 64 is connected to the controller 4, and the controller 4 is used to control the opening or closing of the fourth transfer pump 64.

[0066] Specifically, as Figure 2 shown, the third transfer pump 63 is connected to one of the signal output terminals Y5 of the controller 4 through a wire, and the controller 4 controls the opening or closing of the third transfer pump 63.

[0067] In this embodiment, the energy substance supply tank 12 is used to transport the energy substance - sulfate solution required for the growth of sulfate-reducing bacteria in the facultative anaerobe incubator 11 to the facultative anaerobe incubator 11, and the controller 4 is used to control the addition amount of the energy substance; specifically, when energy substance is required for culturing sulfate-reducing bacteria, the controller 4 controls the opening of the third transfer pump 63, then the sulfate solution in the energy substance supply tank 12 flows out from the third discharge port 121, and after the third transfer pump 63 is opened, the sulfate solution flows into the facultative anaerobe incubator 11 through the third main pipe 53 from the feed inlet 112 above the facultative anaerobe incubator 11.

[0068] Specifically, as Figure 2As shown in the figure, the fourth delivery pump 64 is connected to one of the signal output terminals Y6 of the controller 4 through a wire, and the controller 4 controls the opening or closing of the fourth delivery pump 64; the first valve 71 is connected to one of the signal output terminals Y7 of the controller 4 through a wire, and the controller 4 controls the opening or closing of the first valve 71.

[0069] Since the facultative anaerobe culture device 1 includes an energy substance supply tank 12 and a circulation water tank 13, and a cubic water tank 33 is provided at the bottom of the contaminated pilot box 3, the device provided in this embodiment is better used for the combined remediation of heavy metal contaminated soil by phosphate-solubilizing bacteria and sulfate-reducing bacteria. Specifically, the energy substance supply tank 12 is used to provide sulfate radicals required for the growth of sulfate-reducing bacteria in the facultative anaerobe culture box 11 at the initial stage of cultivation, and the circulation water tank 13 and the cubic water tank 33 are used to continuously provide sulfate radicals required for the growth of sulfate-reducing bacteria during the cultivation process. Therefore, the device provided by the present utility model is better used for the remediation of heavy metal contaminated soil by sulfate-reducing bacteria; further, since the effect of the combined in-situ remediation of heavy metal contaminated soil by phosphate-solubilizing bacteria and sulfate-reducing bacteria is better, the device provided by the present utility model is better used for the combined in-situ remediation of heavy metal contaminated soil by phosphate-solubilizing bacteria and sulfate-reducing bacteria.

[0070] During the process of cultivating sulfate-reducing bacteria, when the sulfate-reducing bacteria in the facultative anaerobe culture box 11 still need sulfate solution for their growth, in this embodiment, there is no need to additionally supplement sulfate radicals through the energy substance supply tank 12, and sulfate radicals can be directly obtained through the internal circulation method for their growth. Specifically, after the heavy metal contaminated soil in the contaminated pilot box 3 is sprayed with the bacterial solution containing phosphate-solubilizing bacteria once and injected with the bacterial solution containing sulfate-reducing bacteria once, the two bacterial solutions react with the heavy metal contaminated soil to perform a primary remediation on the soil, and the reaction effluent containing a certain concentration of sulfate radicals is obtained after the primary remediation; the reaction effluent flows into the cubic water tank 33 through the drain hole 331 at the top of the cubic water tank 33, the controller 4 controls the first valve 71 to open, the reaction effluent flows out from the second water outlet 332 of the cubic water tank 33, and flows into the circulation water tank 13 from the water inlet 131 through the water outlet pipe 54; the controller 4 controls the fourth delivery pump 64 to open, the reaction effluent flows out from the first water outlet 132 of the circulation water tank 13, and the reaction effluent is transported to the feed port 112 of the facultative anaerobe culture box 11 through the fourth main pipe 55, and the reaction effluent is used to provide sulfate radicals for the sulfate-reducing bacteria in the facultative anaerobe culture box 11 for their growth.

[0071] If the sulfate radicals in the facultative anaerobe culture box 11 are sufficient to provide the energy substances required by the sulfate-reducing bacteria, the controller 4 controls the fourth delivery pump 64 to close, and the circulation water tank 13 stops transporting the reaction effluent to the facultative anaerobe culture box 11.

[0072] In some embodiments of the present utility model, a first liquid level gauge 34 is disposed inside the cubic water tank 33. The first liquid level gauge 8-1 / 34 is connected to the controller 4. The controller 4 is configured to receive a first liquid level signal sent by the first liquid level gauge 34 and control the opening or closing of the first valve 71 according to the first liquid level signal.

[0073] A second liquid level gauge 133 is disposed inside the circulation water tank 13. The second liquid level gauge 133 is connected to the controller 4. The controller 4 is configured to receive a second liquid level signal sent by the second liquid level gauge 133 and control the opening or closing of the fourth delivery pump 64 according to the second liquid level signal.

[0074] Wherein, the first liquid level signal is a high limit liquid level signal or a low limit liquid level signal, and the second liquid level signal is a high limit liquid level signal or a low limit liquid level signal; as Figure 2 shown, the first liquid level gauge 34 is connected to one of the signal input terminals Y18 of the controller 4 through a wire, and the second liquid level gauge 133 is connected to one of the signal input terminals Y17 of the controller 4 through a wire.

[0075] In this embodiment, liquid level gauges 8-1 are disposed inside both the cubic water tank 33 and the circulation water tank 13. The liquid level gauges 8-1 are used to detect the liquid level of the reacted water inside the cubic water tank 33 and the circulation water tank 13 and determine whether to send a liquid level signal to the controller 4.

[0076] When the liquid level of the reacted water inside the cubic water tank 33 reaches the high limit, the first liquid level signal sent by the first liquid level gauge 34 is a high limit liquid level signal. The controller 4 receives this high limit liquid level signal and opens the first valve 71 according to this high limit liquid level signal. When the liquid level of the reacted water inside the cubic water tank 33 reaches the low limit, the first liquid level signal sent by the first liquid level gauge 34 is a low limit liquid level signal. The controller 4 receives this low limit liquid level signal and closes the first valve 71 according to this low limit liquid level signal.

[0077] When the liquid level of the reacted water inside the circulation water tank 13 reaches the high limit, the second liquid level signal sent by the second liquid level gauge 133 is a high limit liquid level signal. The controller 4 receives this high limit liquid level signal and opens the fourth delivery pump 64 according to this high limit liquid level signal. When the liquid level of the reacted water inside the cubic water tank 33 reaches the low limit, the second liquid level signal sent by the second liquid level gauge 133 is a low limit liquid level signal. The controller 4 receives this low limit liquid level signal and closes the fourth delivery pump 64 according to this low limit liquid level signal.

[0078] In this embodiment, the high limit and low limit of the liquid levels of the cubic water tank 33 and the circulation water tank 13 are not specifically limited herein and can be set according to the actual application scenario.

[0079] In some embodiments of the present utility model, a first chemical addition port 113 is further provided at the upper end of the facultative anaerobe incubator 11. A second valve 72 is provided on the first chemical addition port 113. The second valve 72 is connected to the controller 4. The controller 4 is used to control the opening or closing of the second valve 72. A second chemical addition port 22 is provided at the upper end of the aerobe incubator 2. A third valve 73 is provided on the second chemical addition port 22. The third valve 73 is connected to the controller 4. The controller 4 is used to control the opening or closing of the third valve 73.

[0080] Agitators 8-2 are provided at the centers of the upper ends of the facultative anaerobe incubator 11, the energy source supply tank 12, and the aerobe incubator 2. The agitator consists of a motor 8-21, an agitator shaft 8-22, and agitator blades 8-23. The motor 8-21 is connected to the agitator blades 8-23 through the agitator shaft 8-22. The motor 8-21 is connected to the controller 4. The controller 4 is used to control the opening or closing of the motor 8-21.

[0081] In this embodiment, nutrients required for the growth of bacteria are added to the incubator through the first chemical addition port 113 and the second chemical addition port 22. Specifically, smooth coatings are provided on the inner walls of the facultative anaerobe incubator 11, the aerobe incubator 2, the inner wall of the first chemical addition port 113, and the inner wall of the second chemical addition port 22. Since the inner walls of the first chemical addition port 113 and the second chemical addition port 22 are smooth, sticking of the applied medicament (such as powdered nutrients) to the wall can be avoided, which affects the proportion of the medicament input.

[0082] In this embodiment, a vertical agitator 8-2 is provided at the center of the upper end of each of the facultative anaerobe incubator 11, the energy source supply tank 12, and the aerobe incubator 2. The motors 8-21 of the agitators 8-2 are all located above the box body. The agitator blades 8-23 of the agitators 8-2 are all close to the bottom of the box body. The agitator 8-2 is used to stir evenly the bacterial liquid in the facultative anaerobe incubator 11 and the aerobe incubator 2 and the sulfate added to the energy source supply tank 12.

[0083] In some embodiments of the present utility model, an energy source detector 8-3 and a first pH meter 8-51 are provided in the facultative anaerobe incubator 11. Both the energy source detector 8-3 and the first pH meter 8-51 are connected to the controller 4. A second pH meter 8-52 is provided in the aerobe incubator 2. The second pH meter 8-52 is connected to the controller 4.

[0084] The controller 4 is configured to receive the detection signal sent by the energy substance detector 8-3 and control the opening or closing of the third delivery pump 63 according to the detection signal; the controller 4 is configured to receive the pH signal sent by the first pH meter 8-51 and / or the second pH meter 8-52 and control the opening or closing of the second valve 72 and / or the third valve 73 according to the pH signal.

[0085] As Figure 2 shown, the third delivery pump 63 is connected to one of the signal output terminals Y5 of the controller 4 through a wire, and the second valve 72 and / or the third valve 73 are connected to the signal output terminals Y8 / Y9 of the controller 4 through a wire; the energy substance detector 8-3 is connected to one of the signal input terminals Y16 of the controller 4 through a wire, and the first pH meter 8-51 and / or the second pH meter 8-52 are connected to the signal input terminals Y19 / Y20 of the controller 4 through a wire.

[0086] Since the energy substance sulfate radical is a key indicator for the growth of sulfate-reducing bacteria, an energy substance detector 8-3 is provided in the facultative anaerobe incubator 11; in addition, the pH value for the growth of sulfate-reducing bacteria is also a key indicator for the growth of sulfate-reducing bacteria, so a first pH meter 8-51 is also provided in the facultative anaerobe incubator 11.

[0087] Since the pH value for the growth of phosphate-solubilizing bacteria is a key indicator for the growth of phosphate-solubilizing bacteria, a second pH meter 8-52 is also provided in the aerobic bacteria incubator 2.

[0088] Among them, the detection signal sent by the energy substance detector 8-3 is a low limit detection signal or a qualified limit detection signal. When the controller 4 receives the low limit detection signal, it opens the third delivery pump 63 according to the low limit detection signal; when the controller 4 receives the qualified limit detection signal, it closes the third delivery pump 63 according to the qualified limit detection signal.

[0089] Among them, the pH signal sent by the first pH meter 8-51 and / or the second pH meter 8-52 is an unqualified limit detection signal (divided into a low limit pH signal and a high limit pH signal) or a qualified limit detection signal. Taking the first pH meter 8-51 as an example, when the controller 4 receives the unqualified limit detection signal of the first pH meter 8-51, the controller 4 adds a pH regulator by opening the second valve 72 according to the unqualified limit detection signal; when the current pH value of the bacterial liquid meets the requirements for microbial growth, the first pH meter 8-51 sends a qualified limit detection signal to the controller 4, and the controller 4 closes the second valve 72 according to the qualified limit detection signal.

[0090] The device provided in this embodiment can monitor the growth status of microorganisms and the culture environment in real time through the signals fed back by the pH meter and the energy substance detector, and use the controller 4 to supplement the energy substance in real time and adjust the pH value of the culture system, with high automation degree and greatly saving labor costs.

[0091] In some embodiments of the present utility model, a first temperature sensor 8-41 is provided inside the facultative anaerobic incubator 11, and a second temperature sensor 8-42 is provided inside the aerobic incubator 2. The controller is respectively connected to the first temperature sensor 8-41 and the second temperature sensor 8-42, and the controller is used to receive the temperature signals sent by the first temperature sensor 8-41 and / or the second temperature sensor 8-42;

[0092] Electric heating devices 8-6 are provided at the bottoms of both the facultative anaerobic incubator 11 and the aerobic incubator 2; the electric heating device 8-6 is connected to the controller 4, and the controller 4 is used to control the opening or closing of the electric heating device 8-6 according to the temperature signal; and

[0093] Heat preservation interlayers 8-7 are arranged around both the facultative anaerobic incubator 3 and the aerobic incubator 2.

[0094] Among them, a first electric heating device 8-6 is provided at the bottom of the facultative anaerobic incubator 11, and a second electric heating device 8-6 is provided at the bottom of the aerobic incubator 2.

[0095] As Figure 2 shown, the first temperature sensor 8-41 is connected to one of the signal input terminals Y21 of the controller 4 through a wire, and the second temperature sensor 8-42 is connected to one of the signal input terminals Y22 of the controller 4 through a wire; the first electric heating device 8-6 is connected to one of the signal output terminals Y14 of the controller 4 through a wire, and the second electric heating device 8-6 is connected to one of the signal output terminals Y15 of the controller 4 through a wire.

[0096] Taking the first temperature sensor 8-41 and the first electric heating device 8-6 in the facultative anaerobic incubator 11 as an example, the temperature adjustment in the device provided in this embodiment will be described.

[0097] The temperature signal sent by the first temperature sensor 8-41 is a low-limit temperature signal or a qualified temperature signal; when the temperature signal sent by the first temperature sensor 8-41 is a low-limit temperature signal, it indicates that the temperature in the facultative anaerobic incubator 11 at this time cannot meet the normal growth of bacteria. Therefore, the controller 4 receives this low-limit temperature signal and turns on the first electric heating device 8-6 of the facultative anaerobic incubator 11 according to this low-limit temperature signal; after turning on the first electric heating device 8-6, when the first temperature sensor 8-41 detects that the temperature in the facultative anaerobic incubator 11 can meet the normal growth of bacteria, it sends a qualified temperature signal to the controller 4, and the controller 4 turns off the first electric heating device 8-6 according to this qualified temperature signal.

[0098] The device provided in this embodiment can monitor the growth state of microorganisms and the culture environment in real time through the signals fed back by the temperature sensor, pH meter, and energy substance detector, and use the controller 4 to supplement the energy substance in real time and adjust the pH value and temperature of the culture system, with a high degree of automation and greatly saving labor costs.

[0099] In addition, both the facultative anaerobic incubator 11 and the aerobic bacteria incubator 2 in the device provided in this embodiment are provided with an electric heating device 8-6 and a heat preservation interlayer 8-7. Among them, the electric heating device 8-6 is used to ensure the appropriate temperature required for the growth of microorganisms, and the heat preservation interlayer 8-7 is used to prevent the heat dissipation of the electric heating device 8-6.

[0100] Using the device provided by the present utility model, the sulfate-reducing bacteria in the facultative anaerobic incubator 11 can always maintain a vigorous growth state in an environment with a certain pH, a certain temperature condition, sufficient energy substances and nutrients, and the phosphate-solubilizing bacteria in the aerobic bacteria incubator 2 can always maintain a vigorous growth state in an environment with a certain pH, a certain temperature condition, and sufficient nutrients, realizing the continuous culture of the sulfate-reducing bacteria solution and the phosphate-solubilizing bacteria solution. Specifically, when the controller 4 receives that the pH, temperature, and sulfate content of the sulfate-reducing bacteria solution in the facultative anaerobic incubator 11 cannot meet its vigorous growth, or receives that the pH, temperature, and sulfate content of the phosphate-solubilizing bacteria solution in the aerobic bacteria incubator 2 cannot meet its vigorous growth, the controller 4 controls the adjustment of the pH, temperature, and sulfate in the box body, so that the microorganisms can always maintain a vigorous growth state; further, in addition to the controller 4 controlling the opening of the third delivery pump 63 to directly supply from the energy substance supply tank 12 through the third main pipe 53 for the adjustment of sulfate, it can also be obtained through the internal circulation method. It should also be noted that nutrients can be added manually through the first medicine adding port 113 and the second medicine adding port 22.

[0101] In this embodiment, the inner walls of the first medicine adding port 113 and the second medicine adding port 22 are smooth, thereby avoiding sticking to the wall when putting in the medicine and affecting the proportion of the medicine input.

[0102] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "illustrative embodiments", "examples", "specific examples", or "some examples", etc. means that the specific features, structures, materials, or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present utility model. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0103] Although the embodiments of the present utility model have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present utility model, and the scope of the present utility model is defined by the claims and their equivalents.

Claims

1. An automated integrated device for repairing heavy metal contaminated soil, characterized in that, The automated integration device includes: a facultative anaerobic bacteria culture device, an aerobic bacteria incubator, a pollution pilot test box, and a controller; the facultative anaerobic bacteria culture device includes a facultative anaerobic bacteria incubator and an energy substance supply box; Heavy metal contaminated soil is placed in the pollution pilot test box, and the pollution pilot test box is connected to the facultative anaerobic bacteria incubator through a first main pipe, and a first transfer pump is arranged on the first main pipe; The pollution pilot test box is connected to the aerobic bacteria incubator through a second main pipe, and a second transfer pump is arranged on the second main pipe; The controller is respectively connected to the first transfer pump and the second transfer pump, and the controller is used to control the opening or closing of the first transfer pump and / or the second transfer pump.

2. The automated integration device according to claim 1, wherein The pollution pilot test box includes at least one cylindrical pipe, and the upper end of the cylindrical pipe is connected to the first main pipe; A first discharge port is arranged at the lower end of the facultative anaerobic bacteria incubator, one end of the first transfer pump is connected to the first discharge port, and the other end is connected to the first main pipe.

3. The automated integration device according to claim 2, wherein, The cylindrical pipe is vertically arranged inside the pollution pilot test box and is buried in the heavy metal contaminated soil; At least one small hole is longitudinally distributed on the pipe wall of the cylindrical pipe; the bottom of the cylindrical pipe is sealed, and at least one layer of gauze is wrapped on the side.

4. The automated integration device according to claim 1, characterized in that, The pollution pilot test box includes at least one spray pipe, the spray pipe is arranged at the top of the pollution pilot test box, and the spray pipe is connected to the second main pipe; A second discharge port is arranged at the lower end of the aerobic bacteria incubator, one end of the second transfer pump is connected to the second discharge port, and the other end is connected to the second main pipe.

5. The automated integration device according to claim 4, characterized in that The spray pipe (32) is arranged in a grid shape.

6. The automated integration device according to claim 1, wherein, A feed port is arranged above the facultative anaerobic bacteria incubator; a third discharge port is arranged at the lower end of the energy substance supply box, and the third discharge port is connected to the feed port above the facultative anaerobic bacteria incubator through a third main pipe; a third transfer pump is arranged on the third main pipe, the third transfer pump is connected to the controller, and the controller is used to control the opening or closing of the third transfer pump; The facultative anaerobic bacteria culture device includes a circulation water tank, an inlet is arranged above the circulation water tank, and a first water outlet is arranged at the lower end; A cubic water tank is arranged at the bottom inside the pollution pilot test box, and the heavy metal contaminated soil is placed on the cubic water tank; a drain hole is arranged at the top of the cubic water tank, a second water outlet is arranged at the bottom, and the second water outlet is connected to the inlet through a water outlet pipe; a first valve is arranged on the water outlet pipe, the first valve is connected to the controller, and the controller is used to control the opening or closing of the first valve; The first water outlet is connected to the feed port through a fourth main pipe, a fourth circulation pump is arranged on the fourth main pipe, the fourth circulation pump is connected to the controller, and the controller is used to control the opening or closing of the fourth circulation pump.

7. The automated integration device according to claim 6, wherein A first liquid level gauge is arranged inside the cubic water tank, the first liquid level gauge is connected to the controller, and the controller is used to receive a first liquid level signal sent by the first liquid level gauge and control the opening or closing of the first valve according to the first liquid level signal; A second liquid level gauge is provided inside the circulating water tank. The second liquid level gauge is connected to the controller. The controller is configured to receive the second liquid level signal sent by the second liquid level gauge and control the opening or closing of the fourth delivery pump according to the second liquid level signal.

8. The automated integration device according to claim 1, characterized in that, A first chemical addition port is further provided at the upper end of the facultative anaerobe incubator. A second valve is provided on the first chemical addition port. The second valve is connected to the controller. The controller is configured to control the opening or closing of the second valve. A second chemical addition port is provided at the upper end of the aerobe incubator. A third valve is provided on the second chemical addition port. The third valve is connected to the controller. The controller is configured to control the opening or closing of the third valve. Agitators are provided at the centers of the upper ends of the facultative anaerobe incubator, the energy substance supply tank, and the aerobe incubator. The agitator consists of a motor, a stirring shaft, and stirring blades. The motor is connected to the stirring blades through the stirring shaft. The motor is connected to the controller. The controller is configured to control the opening or closing of the motor.

9. The automated integration device according to claim 1, wherein An energy substance detector and a first pH meter are provided inside the facultative anaerobe incubator. Both the energy substance detector and the first pH meter are connected to the controller. A second pH meter is provided inside the aerobe incubator. The second pH meter is connected to the controller. The controller is configured to receive the detection signal sent by the energy substance detector and control the opening or closing of the third delivery pump according to the detection signal. The controller is configured to receive the pH signal sent by the first pH meter and / or the second pH meter and control the opening or closing of the second valve and / or the third valve according to the pH signal.

10. The automated integration device according to claim 1, characterized in that A first temperature sensor is provided inside the facultative anaerobe incubator. A second temperature sensor is provided inside the aerobe incubator. The controller is respectively connected to the first temperature sensor and the second temperature sensor. The controller is configured to receive the temperature signal sent by the first temperature sensor and / or the second temperature sensor. Electric heating devices are provided at the bottoms of both the facultative anaerobe incubator and the aerobe incubator. The electric heating devices are connected to the controller. The controller is configured to control the opening or closing of the electric heating devices according to the temperature signal. Heat preservation interlayers are provided around both the facultative anaerobe incubator and the aerobe incubator.

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