Cell magnetic bead classifier

Abstract

The utility model discloses a cell magnetic bead sorter, which belongs to the technical field of magnetic bead sorting and comprises a magnetic separation base, a moving mechanism is arranged on one side of the magnetic separation base, and a dispersing mechanism is arranged at the top of the magnetic separation base. The moving mechanism comprises a separation plate fixedly connected between the top of the magnetic separation base and the moving mechanism, a moving rail is fixedly connected to one side of the separation plate, a moving block is slidably connected to the inner wall of the moving rail, a connecting rod is fixedly connected to the top of the moving block, and a connecting plate is fixedly connected to the bottom of the connecting rod; the bottom of the connecting plate makes contact with the inner wall of the bottom of the sorting plate and is in sliding fit with the inner wall of the bottom of the sorting plate, the inner wall of one end of the moving rail is rotationally connected with a threaded rod through a bearing, the threaded rod is in threaded connection with the moving block, and one end of the threaded rod is fixedly connected with a rotating block. According to the utility model, the effect of facilitating operation of workers can be realized.

Description

Technical Field

[0001] The utility model belongs to the technical field of magnetic bead sorting, and particularly relates to a cell magnetic bead sorter. Background Art

[0002] A cell magnetic bead sorter is a laboratory device used for rapidly and efficiently sorting cells labeled with magnetic beads. This method is called magnetic bead sorting, which is a non-invasive and non-damaging cell sorting technique. The basic principle of magnetic bead sorting is to bind magnetic particles (magnetic beads) to specific markers (such as antibodies or proteins) on the surface of target cells (such as specific types of immune cells, stem cells, or cancer cells), and then use a magnetic field to separate the labeled cells from the unlabeled cells.

[0003] Currently, a Chinese patent with the publication number CN207031447U discloses a cell magnetic bead sorter, belonging to the technical field of magnetic sample separation devices, which is designed to solve problems such as slow screening speed of existing cell samples and inapplicability of magnetic bead sorting of magnetic separators to trace cell samples.

[0004] However, when the above device is used, it relies on gravity to screen the magnetic selected cell samples. After the screening is completed and the non-magnetic cell samples are discharged, the magnetic cells are difficult to move on the sealing film, which is not convenient for the operation of the staff. Content of the Utility Model

[0005] The purpose of the utility model is to solve the disadvantages existing in the prior art, and a cell magnetic bead sorter is proposed. Its advantages are as follows: the effect of facilitating the operation of the staff can be achieved.

[0006] In order to achieve the above purpose, the utility model adopts the following technical scheme:

[0007] A cell magnetic bead sorter includes a magnetic separation base, a moving mechanism is arranged on one side of the magnetic separation base, and a dispersion mechanism is arranged on the top of the magnetic separation base;

[0008] The moving mechanism includes a sorting plate fixedly connected between the top of the magnetic separation base and the sorting plate, a moving rail is fixedly connected to one side of the sorting plate, a moving block is slidably connected to the inner wall of the moving rail, a connecting rod is fixedly connected to the top of the moving block, a connecting plate is fixedly connected to the bottom of the connecting rod, and the bottom of the connecting plate is in contact with and forms a sliding fit with the bottom inner wall of the sorting plate.

[0009] Through the above technical solution: When in use, rotating the rotating block can cause the threaded rod to rotate. The rotation of the threaded rod can cause the moving block to slide inside the moving rail. The movement of the moving block can cause the connecting plate to move and scrape and collect the magnetic cells on the sorting plate. When non-magnetic cells are mixed in the middle position of the magnetic cells, the positioning plate can be rotated to make the baffle contact the inner wall of the bottom of the sorting plate. Rotating the turning handle can cause the rotating shaft to rotate. The rotation of the rotating shaft can cause the rotating plate to rotate. The rotation of the rotating plate can disperse the cells, making the magnetic cells adjacent to each other. Therefore, the effect of facilitating the operation of the staff can be achieved.

[0010] The utility model is further arranged such that one end inner wall of the moving rail is rotatably connected to a threaded rod through a bearing, and the threaded rod is threadedly connected to the moving block.

[0011] Through the above technical solution: The setting of the threaded rod can cause the moving block to slide inside the moving rail when rotating, and at this time, the connecting plate can be moved.

[0012] The utility model is further arranged such that one end of the threaded rod is fixedly connected to a rotating block.

[0013] Through the above technical solution: The setting of the rotating block can cause the threaded rod to rotate when rotating, and the rotation of the threaded rod can cause the moving block to move.

[0014] The utility model is further arranged such that the dispersion mechanism includes a rotatable rotating shaft, and both sides of the rotating shaft are fixedly connected with rotating plates, and a sliding fit is formed between the bottom of the rotating plate and the inner wall of the bottom of the sorting plate.

[0015] Through the above technical solution: The setting of the rotating plate can rotate when the rotating shaft rotates, and the rotating plate can separate non-magnetic cells from magnetic cells.

[0016] The utility model is further arranged such that one end of the connecting plate is provided with a baffle, and a sliding fit is formed between the baffle and the inner wall of the bottom of the sorting plate.

[0017] Through the above technical solution: The setting of the baffle can make the sample located between the connecting plate and the baffle, and can prevent the cells from splashing out.

[0018] The utility model is further arranged such that the top of the baffle is fixedly connected with a positioning plate, and the positioning plate is rotatably connected to the rotating shaft through a bearing.

[0019] Through the above technical solution: The setting of the positioning plate can limit the rotating shaft and enable the rotating shaft to rotate horizontally.

[0020] The utility model is further arranged such that the top of the rotating shaft is fixedly connected with a turning handle.

[0021] Through the above technical solution: the setting of the turning handle can make the rotating shaft rotate when it rotates, and the rotation of the rotating shaft can make the rotating plate rotate.

[0022] The utility model is further configured such that a connection port is provided at the top of the connecting rod, and the same connecting shaft is rotatably connected between the inner walls on both sides of the connection port through a bearing, and the connecting shaft is fixedly connected to the positioning plate.

[0023] Through the above technical solution: the setting of the connecting shaft can make the positioning plate rotate when it rotates, and it is convenient to adjust the position of the baffle.

[0024] The beneficial effects of the utility model are as follows:

[0025] 1. For this cell magnetic bead sorter, the rotation of the threaded rod can be achieved through the rotating block, the rotation of the threaded rod can make the moving block slide in the inner wall of the moving rail, and the movement of the moving block can make the connecting plate move to scrape and collect the magnetic cells on the sorting plate. Therefore, the effect of facilitating the operation of the staff can be achieved.

[0026] 2. For this cell magnetic bead sorter, by rotating the positioning plate to make the baffle contact the inner wall of the bottom of the sorting plate, rotating the turning handle can make the rotating shaft rotate, the rotation of the rotating shaft can make the rotating plate rotate, and the rotation of the rotating plate can disperse the cells, making the magnetic cells adjacent to each other. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 FIG. is a three-dimensional structural schematic diagram of a cell magnetic bead sorter proposed by the utility model;

[0028] Figure 2 FIG. is a structural schematic diagram of a cell magnetic bead sorter proposed by the utility model highlighting the connecting plate;

[0029] Figure 3 is Figure 2 an enlarged structural schematic diagram at A in

[0030] Figure 4 FIG. is a structural schematic diagram of a cell magnetic bead sorter proposed by the utility model highlighting the rotating shaft.

[0031] In the figure: 1, magnetic separation base; 2, sorting plate; 3, moving rail; 4, moving block; 5, connecting rod; 6, connecting plate; 7, threaded rod; 8, rotating block; 9, rotating shaft; 10, rotating plate; 11, baffle; 12, positioning plate; 13, connection port; 14, connecting shaft; 15, turning handle. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0032] The technical solutions of this patent will be further described in detail below in conjunction with the specific embodiments.

[0033] The embodiments of this patent will be described in detail below. Examples of the embodiments are shown in the accompanying drawings, where the same or similar reference numerals denote the same or similar elements or elements with the same or similar functions throughout. The embodiments described below by referring to the accompanying drawings are exemplary and are only used to explain this patent and should not be construed as a limitation of this patent.

[0034] In the description of this patent, it should be understood that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the accompanying drawings. It is only for the convenience of describing this patent and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this patent.

[0035] In the description of this patent, it should be noted that unless otherwise clearly specified and defined, the terms "installed", "connected", "connected to", "set" should be understood in a broad sense. For example, it can be fixedly connected and set, or detachably connected and set, or integrally connected and set. For those of ordinary skill in the art, the specific meanings of the above terms in this patent can be understood according to specific circumstances.

[0036] Refer to Figures 1-4 , a cell magnetic bead sorter, which includes a magnetic separation base 1. A moving mechanism is provided on one side of the magnetic separation base 1, and a dispersion mechanism is provided on the top of the magnetic separation base 1;

[0037] The moving mechanism includes a sorting plate 2 fixedly connected to the top of the magnetic separation base 1. A moving rail 3 is fixedly connected to one side of the sorting plate 2. A moving block 4 is slidably connected to the inner wall of the moving rail 3. A connecting rod 5 is fixedly connected to the top of the moving block 4. A connecting plate 6 is fixedly connected to the bottom of the connecting rod 5. The bottom of the connecting plate 6 is in contact with the inner wall of the bottom of the sorting plate 2 and forms a sliding fit.

[0038] It should be noted that in this embodiment, one end of the inner wall of the moving rail 3 is rotatably connected to a threaded rod 7 through a bearing. The threaded rod 7 is threadedly connected to the moving block 4. By rotating the threaded rod 7, the moving block 4 can slide in the inner wall of the moving rail 3, and at this time, the connecting plate 6 can be moved.

[0039] Furthermore, in this embodiment, a rotating block 8 is fixedly connected to one end of the threaded rod 7. By rotating the rotating block 8, the threaded rod 7 can be rotated, and the rotation of the threaded rod 7 can move the moving block 4.

[0040] In one specific embodiment, the dispersion mechanism includes a rotatable rotating shaft 9. Rotating plates 10 are fixedly connected to both sides of the rotating shaft 9. A sliding fit is formed between the bottom of the rotating plate 10 and the bottom inner wall of the sorting plate 2. The rotating plate 10 can rotate when the rotating shaft 9 rotates. The rotating plate 10 can separate non-magnetic cells from magnetic cells.

[0041] It should be noted that in this embodiment, a baffle 11 is provided at one end of the connecting plate 6. A sliding fit is formed between the baffle 11 and the bottom inner wall of the sorting plate 2. The baffle 11 can make the sample located between the connecting plate 6 and the baffle 11, and can prevent the cells from splashing out.

[0042] Furthermore, in this embodiment, a positioning plate 12 is fixedly connected to the top of the baffle 11. The positioning plate 12 is rotatably connected to the rotating shaft 9 through a bearing. The positioning plate 12 can limit the rotating shaft 9, and can make the rotating shaft 9 rotate horizontally.

[0043] A turning handle 15 is fixedly connected to the top of the rotating shaft 9. The turning handle 15 can make the rotating shaft 9 rotate when rotating. The rotation of the rotating shaft 9 can make the rotating plate 10 rotate.

[0044] A connection port 13 is opened at the top of the connecting rod 5. The same connecting shaft 14 is rotatably connected between the inner walls on both sides of the connection port 13. The connecting shaft 14 is fixedly connected to the positioning plate 12. The connecting shaft 14 can make the positioning plate 12 rotate when rotating, and can facilitate the adjustment of the position of the baffle 11.

[0045] Working principle: When in use, rotating the rotating block 8 can make the threaded rod 7 rotate. The rotation of the threaded rod 7 can make the moving block 4 slide in the inner wall of the moving rail 3. The movement of the moving block 4 can make the connecting plate 6 move to scrape and collect the magnetic cells on the sorting plate 2. When non-magnetic cells are mixed in the middle position of the magnetic cells, the positioning plate 12 can be rotated to make the baffle 11 contact with the bottom inner wall of the sorting plate 2. Rotating the turning handle 15 can make the rotating shaft 9 rotate. The rotation of the rotating shaft 9 can make the rotating plate 10 rotate. The rotation of the rotating plate 10 can disperse the cells, and can make the magnetic cells adjacent to each other. Therefore, the effect of facilitating the operation of the staff can be achieved.

[0046] The above is only the preferred specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention, according to the technical solution and the inventive concept of the present invention, makes equivalent substitutions or changes, and should be covered within the protection scope of the present invention.

Claims

1. A cell magnetic bead sorter, comprising a magnetic separation base (1), characterized in that, A moving mechanism is provided on one side of the magnetic separation base (1), and a dispersing mechanism is provided on the top of the magnetic separation base (1). The moving mechanism includes a sorting plate (2) fixedly connected to the top of the magnetic separation base (1). A moving rail (3) is fixedly connected to one side of the sorting plate (2). A moving block (4) is slidably connected to the inner wall of the moving rail (3). A connecting rod (5) is fixedly connected to the top of the moving block (4). A connecting plate (6) is fixedly connected to the bottom of the connecting rod (5). The bottom of the connecting plate (6) is in contact with and forms a sliding fit with the inner bottom wall of the sorting plate (2).

2. The cell magnetic bead sorter according to claim 1, wherein One end of the inner wall of the moving rail (3) is rotatably connected to a threaded rod (7) through a bearing. The threaded rod (7) is threadedly connected to the moving block (4).

3. The cell magnetic bead sorter according to claim 2, characterized in that, One end of the threaded rod (7) is fixedly connected to a rotating block (8).

4. A cell magnetic bead sorter according to claim 1, characterized in that, The dispersing mechanism includes a rotatable rotating shaft (9). Rotating plates (10) are fixedly connected to both sides of the rotating shaft (9). The bottom of the rotating plate (10) forms a sliding fit with the inner bottom wall of the sorting plate (2).

5. A cell magnetic bead sorter according to claim 4, characterized in that, A baffle (11) is provided at one end of the connecting plate (6). The baffle (11) forms a sliding fit with the inner bottom wall of the sorting plate (2).

6. The cell magnetic bead sorter according to claim 5, wherein, A positioning plate (12) is fixedly connected to the top of the baffle (11). The positioning plate (12) is rotatably connected to the rotating shaft (9) through a bearing.

7. The cell magnetic bead sorter according to claim 6, wherein A turning handle (15) is fixedly connected to the top of the rotating shaft (9).

8. A cell magnetic bead sorter according to claim 6, characterized in that, A connection port (13) is opened at the top of the connecting rod (5). The same connecting shaft (14) is rotatably connected between the inner side walls of both sides of the connection port (13). The connecting shaft (14) is fixedly connected to the positioning plate (12).

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