Distribution liquid flow distribution plate for large-scale chromatographic column, distribution assembly and chromatographic column thereof

Abstract

The utility model provides a distribution liquid flow distribution plate for a large-scale chromatographic column, a distribution assembly and a chromatographic column thereof, the distribution liquid flow distribution plate comprises a distribution plate, a plurality of inwards concave distribution grooves are uniformly distributed on one surface of the plate body, and the cross section of the opening of each distribution groove is larger than the cross section of the bottom; the plate body is provided with a plurality of shunting holes, and the shunting holes correspond to the distribution grooves and penetrate through the plate body to communicate the distribution grooves with the other surface of the plate body; the other surface of the plate body adopts an open flow channel or a distribution flow channel; the distribution assembly comprises the distribution plate, and the distribution plate is placed in a groove of a column flange or a piston. One surface of the distribution plate with the open flow channel or the distribution flow channel is close to the column flange or the piston, and a screen plate is placed on one surface of the distribution plate with the distribution groove; the distribution plate is detachably and fixedly connected with the flange or the piston; the chromatographic column comprises the distribution plate and the distribution assembly; the distribution device has the advantages of good distribution effect and high efficiency.

Description

Technical Field

[0001] The utility model relates to a chromatography device for separation and purification in the field of biomedicine, and particularly relates to a distribution liquid flow distribution plate, a distribution assembly and a chromatography column for a large chromatography column. Background Art

[0002] In the downstream of the biomedicine field, chromatography columns are mainly used to effectively separate target products and remove impurities, so as to obtain the final products, such as biological macromolecules proteins, polypeptides, polysaccharides, ADCs, DNAs and RNAs, etc. The evaluation criteria for the performance of chromatography columns require continuity, stability, uniformity and sealing performance. Therefore, the distribution plate involved in the chromatography column plays an important role. The shunt plates used in the current existing technologies all have diversion grooves and liquid guide holes, which can ensure the uniform distribution of the mobile phase. However, the disadvantages of the current existing technologies are as follows: the diversion grooves are directly formed on the diversion plate, and the liquid entering through the mobile phase inlet directly acts on the sieve plate assembly, and the structure on the sieve plate or the sieve plate assembly makes the liquid change from the vertical flow direction to the horizontal flow direction. If the medium under the sieve plate is not compacted or has gaps, the effect of this shunt structure will be discounted. Summary of the Utility Model

[0003] In order to overcome the deficiencies of the above-mentioned existing technologies, the utility model provides a distribution liquid flow distribution plate, a distribution assembly and a chromatography column for a large chromatography column. It can facilitate the installation of the plunger or flange and the distribution plate, and at the same time enable the mobile phase to be quickly distributed on the cross-section of the packing in the chromatography column to improve the column efficiency.

[0004] In order to achieve the above purpose, the technical solution adopted by the utility model is as follows:

[0005] In view of the above technical problems, the first aspect of the disclosed content of the utility model provides a distribution liquid flow distribution plate for a large chromatography column, including a plate body. A plurality of inwardly recessed distribution grooves are evenly distributed on one side of the plate body, and the cross-sectional opening of the distribution groove is larger than the bottom cross-section; a plurality of shunt holes are provided on the plate body, the shunt holes correspond to the distribution grooves, and penetrate through the plate body to communicate the distribution grooves with the other side of the plate body; the other side of the plate body adopts an open channel or a distribution channel.

[0006] In some embodiments, the distribution grooves are evenly distributed on the plate body in a concentric circle manner, and there are gaps between the distribution grooves.

[0007] In some embodiments, the longitudinal section of the distribution groove is semi-circular, inverted trapezoidal or triangular.

[0008] In some embodiments, the distribution groove adopts a conical structure.

[0009] In some embodiments, the distribution flow channel includes a centrally recessed central groove, and a plurality of concentric circular flow channels radiate outward from the central groove. The diversion holes are evenly distributed on the concentric circular flow channels, and the central groove and the concentric circular flow channels are connected through radial flow channels.

[0010] In some embodiments, the diversion holes are located at the central axis position of the bottom of the distribution groove.

[0011] In view of the above technical problems, the second aspect of the disclosure of the present utility model provides a distribution assembly for a large chromatography column, including the above-mentioned distribution plate, which is placed in the groove of the column flange or the piston; the side of the distribution plate with the open flow channel or the distribution flow channel is close to the column flange or the piston, and a screen plate is placed on the side of the distribution plate with the distribution groove; the distribution plate is detachably and fixedly connected to the flange or the piston.

[0012] In some embodiments, the distribution plate is fixedly connected to the column flange or the piston by screws. The screws pass through the column flange or the piston and the distribution plate, and a sealing ring is provided on the screws between the distribution plate and the column flange or the piston. A sealing ring is provided between the distribution plate and the column flange or the piston and / or between the distribution plate and the screen plate.

[0013] In some embodiments, the sum of the thicknesses of the plate body 1 of the distribution plate and the screen plate 13 is equal to the depth of the groove 9.

[0014] In view of the above technical problems, the third aspect of the disclosure of the present utility model provides a chromatography column, including the above-mentioned distribution plate or distribution assembly.

[0015] Advantages of the present utility model:

[0016] The distribution groove adopted by the present utility model has a design in which the opening diameter is larger than the bottom diameter, which can make the liquid phase more evenly distributed into the chromatography column, achieving the best distribution effect. The open-type flow channel has a simple structure and is easy to install;

[0017] The distribution flow channel adopts a concentric circular radiation flow channel, which can enable the liquid phase coming out of the central groove to quickly reach the distribution groove far from the central groove, improving the high efficiency of the liquid phase flow. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a structural diagram of one side of the distribution plate of the present utility model.

[0019] Figure 2 It is a structural diagram of the other side of the distribution plate of the present utility model, Embodiment 1.

[0020] Figure 3 It is a structural diagram of the other side of the distribution plate of the present utility model, Embodiment 2.

[0021] Figure 4 This is a cross-sectional view of a partial structure of the distribution board of the present utility model.

[0022] Figure 5 This is a schematic diagram of the structure of the distribution groove of the present utility model.

[0023] Figure 6 This is a schematic diagram of the structure of the distribution component of the present utility model. Detailed implementation manners

[0024] The present utility model will be further described below with reference to the accompanying drawings.

[0025] The following describes the technical content of the present utility model through specific specific embodiments. Those skilled in the art can easily understand other advantages and effects of the present utility model from the content disclosed in this specification. The present utility model can also be implemented or applied through other different specific embodiments. Those skilled in the art make various modifications and changes without departing from the spirit of the present utility model.

[0026] Before introducing the specific embodiments of the present disclosure in detail, some terms used in the present disclosure will be described first.

[0027] Unless otherwise defined hereinafter, the meanings of all technical terms and scientific terms used herein are intended to be the same as those commonly understood by those skilled in the art. References to technologies used herein refer to technologies commonly understood in the art, including changes to technologies that are obvious to those skilled in the art or substitutions of equivalent technologies. Although it is believed that the following terms are well understood by those skilled in the art, the following definitions are still set forth to better explain the present utility model. When a trade name appears herein, it is intended to refer to its corresponding product. All patents, published patent applications, and publications cited herein are incorporated herein by reference.

[0028] Unless otherwise specified in the text, multiple referents such as "a", "the", include plural referents. The expression "one or more" or "at least one" can mean 1, 2, 3, 4, 5, 6, 7, 8, 9 or more.

[0029] As used herein, terms such as "connected", "linked", "coupled" or "coupled" and the like are not limited to direct connection, and also include indirect connection.

[0030] As described herein, "liquid phase" refers to biomolecules, including proteins, nucleic acids, lipids, carbohydrates, small nucleotides, amino acids and their derivatives.

[0031] As used herein, the positional relationship terms such as "upper", "lower", "left", "right", "front", and "rear" are determined according to the layout direction of the drawings in the specification, and they are only used to represent the relative positional relationship. When the absolute position of the object being described changes, the relative positional relationship may also change accordingly.

[0032] As shown in Figure 1 the distribution liquid flow distribution plate for a large chromatography column includes a plate body 1. On one side of the plate body 1, a plurality of inwardly recessed distribution grooves 2 are evenly distributed. From Figure 1 it can be seen that the distribution grooves are circular, and a flow splitting hole 3 is provided at the center of each corresponding distribution groove 2. The flow splitting hole penetrates both sides of the plate body 1. The distribution grooves 2 adopt a concentric circle layout method and are evenly distributed on the plate body 1 in a radially outward manner, and there are gaps between the respective distribution grooves 2.

[0033] As shown in Figure 2 the other side of the distribution plate is shown. It adopts an open-type flow channel, that is, the other side of the plate body 1 is a smooth plate surface, on which the corresponding flow splitting holes 3 and screw holes 4 for fixed connection are evenly arranged, and there are 12 screw holes 4.

[0034] As shown in Figure 3 the distribution flow channel adopted on the other side of the distribution plate is shown, which includes an inwardly recessed central groove 5. The central groove 5 radiates a plurality of concentric circle flow channels 6 outward, and there are 8 here. The flow splitting holes 3 are evenly distributed on the concentric circle flow channels 6. The central groove 5 is communicated with the concentric circle flow channels through radial flow channels 7, and there are 12 radial flow channels 7.

[0035] As shown in Figure 4 it includes a body 1. The upper and lower sides of the body 1 are communicated through a flow splitting hole 2, and the flow splitting hole 2 is located at the central axis position of the distribution groove 2. The opening cross-section of the distribution groove 2 is larger than the bottom cross-section, forming a conical structure.

[0036] As shown in Figure 5 the longitudinal section of the distribution groove 2 is an inverted trapezoidal structure, such as a frustum or a truncated cone. Or the cross-section is a semi-circle, such as a hemisphere. Or the longitudinal section is a triangular cone.

[0037] As shown in Figure 6, A distribution component for a large chromatography column, including the distribution plate described above. The distribution plate is placed in the groove 9 of the column flange or the piston 8. The side of the plate body 1 of the distribution plate with open channels or distribution channels is close to the column flange or the piston 8. A screen plate 13 is placed on the side of the plate body 1 with distribution grooves 2; the sum of the thicknesses of the plate body 1 of the distribution plate and the screen plate 13 is equal to the depth of the groove 9. The plate body 1 is fixedly connected to the column flange or the piston 8 by screws 10, and the screws 10 pass through the column flange or the piston 8 to reach the screw holes 4 of the body 1. A first sealing ring 11 is provided between the plate body 1 and the column flange or the piston 8 and between the body 1 and the screen plate 13; a second sealing ring 12 is provided on the screws 10 between the plate body 1 and the column flange or the piston 8.

Claims

1. A distribution plate for distributing liquid flow in a large chromatography column, characterized in that, It includes a plate body, on one side of which a plurality of inwardly recessed distribution grooves are evenly distributed, and the opening cross-section of the distribution grooves is larger than the bottom cross-section; the plate body is provided with a plurality of diversion holes corresponding to the distribution grooves, which penetrate the plate body to connect the distribution grooves with the other side of the plate body; the other side of the plate body adopts an open flow channel or a distribution flow channel.

2. The distribution board according to claim 1, wherein The distribution grooves are evenly distributed on the plate body in a concentric circle manner, and there are gaps between the respective distribution grooves.

3. The distribution board according to claim 1, characterized in that, The longitudinal cross-section of the distribution grooves is semi-circular, inverted trapezoidal or triangular.

4. The distribution board according to claim 1, characterized in that, The distribution grooves adopt a conical structure.

5. The distribution board according to claim 1, wherein The distribution flow channel includes a centrally recessed central groove, and a plurality of concentric circle flow channels radiate outwards from the central groove. The diversion holes are evenly distributed on the concentric circle flow channels, and the central groove and the concentric circle flow channels are connected through radial flow channels.

6. The distribution board according to claim 1, characterized in that, The diversion holes are located at the central axis position of the bottom of the distribution grooves.

7. Distribution component for large chromatography column, characterized in that, It includes the distribution plate described in claims 1-6, and the distribution plate is placed in the groove of the column flange or the piston; the side of the distribution plate with the open flow channel or the distribution flow channel is close to the column flange or the piston, and a screen plate is placed on the side of the distribution plate with the distribution grooves; the distribution plate is detachably and fixedly connected to the flange or the piston.

8. The dispensing assembly for a large chromatography column according to claim 7, characterized in that, The distribution plate is fixedly connected to the column flange or the piston by screws. The screws pass through the column flange or the piston and the distribution plate, and a sealing ring is provided on the screws between the distribution plate and the column flange or the piston.

9. The dispensing assembly for a large chromatography column according to claim 7, wherein, A sealing ring is provided between the distribution plate and the column flange or the piston and / or between the distribution plate and the screen plate.

10. The distribution component for a large chromatography column according to claim 7, characterized in that, The sum of the thicknesses of the plate body of the distribution plate and the screen plate is equal to the depth of the groove.

11. A chromatography column, characterized in that, It includes the distribution plate described in claims 1-6 above or the distribution assembly described in claims 7-10.

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