Novel transfection reagent loading box

Abstract

The utility model relates to the technical field of transfection reagent loading boxes, in particular to a novel transfection reagent loading box which comprises a loading box body with an opening in the bottom, an adjusting opening is connected to the interior of the loading box body through a loading cover body, a containing cavity is formed in the bottom of the adjusting opening, and the containing cavity is communicated with the loading box body. The loading cover body is used for driving the adjusting opening and the containing cavity to longitudinally ascend and descend, sealing pieces are arranged on the two sides of the adjusting opening, a partition plate body and a mounting shaft are fixedly connected to the interiors of the sealing pieces, a placing plate body is slidably connected to the interiors of the sealing pieces, and the placing plate body can make contact with the partition plate body; and the free end of the mounting shaft is fixedly connected with the side wall of the placement plate body, and a connecting spring is slidably arranged at the bottom of the loading box body. According to the utility model, the problem that various different reagents need to be transferred separately and independently in the prior art is effectively solved, and the purpose of simplifying the whole transferring step is achieved.

Description

Technical Field

[0001] The utility model relates to the technical field of transfection reagent loading boxes, and particularly relates to a novel transfection reagent loading box. Background Art

[0002] With the in-depth research of genetic engineering and cell biology, transfection technology has become an important means to study biological processes such as gene expression and cell signal transduction. Transfection reagent loading boxes usually integrate functions such as storage, dilution, and mixing of transfection reagents to ensure the stability and consistency of transfection reagents during use. At the same time, by optimizing the mixing ratio and conditions of transfection reagents with DNA or RNA, the transfection efficiency is improved and the toxicity to cells is reduced.

[0003] Most of the existing novel transfection reagent loading boxes are composed of components such as a box body, a box cover, a placement plate, and a transfection reagent container. During storage, the transfection reagent needs to be safely stored in a specific container inside the loading box to maintain its stability and activity. When needed, the transfection reagent is mixed evenly through a specific mechanism to ensure consistent results for the reagent used in each experiment. After being mixed evenly, the transfection reagent can be conveniently distributed into the experimental container through a specific channel or structure designed in the loading box, or the extraction of the reagent is assisted by components such as a top block. For example, in the Chinese patent with the publication number CN217651185U and the name: A Novel Transfection Reagent Loading Box, by setting a sleeve and pulling the sleeve upward, when the sleeve moves upward, since the sleeve and the cylinder are connected together through a spiral groove and a slider, and the slider slides on the inner wall of the spiral groove, the cylinder is restricted and rotates, thereby driving the transfection reagent inside the cylinder to rotate together to shake the transfection reagent evenly. The sleeve will move together with the connecting rod. When shaking the transfection reagent evenly, the top block will also be pushed upward, so that the reagent on the top of the top block is pushed out, which is convenient for taking and using.

[0004] However, during its use, different types of transfection reagents need to be loaded in different loading boxes to avoid cross-contamination. During the period when there is less reagent, more loading boxes are also needed for separate placement, which makes the overall operation cumbersome and complex, is not conducive to the overall loading and taking operation, and will also occupy a large space during storage. Therefore, this application provides a novel transfection reagent loading box to meet the requirements. Summary of the Utility Model

[0005] The purpose of this application is to provide a novel transfection reagent loading box, which solves the problem that multiple different reagents need to be separately and independently loaded in the prior art, and achieves the purpose of simplifying the overall loading steps.

[0006] To achieve the above object, the present application provides the following technical solutions: A novel transfection reagent loading box, including a loading box body with an open bottom, the inside of the loading box body is connected with an adjustment port through a loading cover body, and a receiving cavity is provided at the bottom of the adjustment port. The loading cover body is used to drive the adjustment port and the receiving cavity to move longitudinally up and down. Seals are provided on both sides of the adjustment port, and a partition plate body and a mounting shaft are fixedly connected inside the seals. A placement plate body is slidably connected inside the seals. The placement plate body can contact the partition plate body, and the free end of the mounting shaft is fixedly connected to the side wall of the placement plate body. A connecting spring is slidably provided at the bottom of the loading box body.

[0007] Preferably, an adjustment groove is provided inside the loading box body, and an adjustment block is provided on the adjustment groove. A sliding groove is slidably connected inside the surgical adjustment block, and the top end of the loading cover body is fixedly connected to the bottom of the sliding groove.

[0008] Preferably, a plurality of sliding members are provided on both sides of the loading box body, the adjustment groove and the sliding groove. A plurality of plug-in grooves are connected inside the loading box body through a plurality of plug-in pins. The plug-in grooves can be plugged inside the sliding members, and a connecting buckle is provided on the circumference of the plug-in grooves. The free end of the plug-in pin is fixedly connected to the connecting buckle.

[0009] Preferably, a connecting scraper is fixedly connected to the bottom of the adjustment port, and a connecting frame is provided on the receiving cavity. The connecting frame is plugged inside the connecting scraper.

[0010] Preferably, an elastic band is fixedly connected inside the connecting scraper, and a sealing sleeve is fixedly connected to the top of the connecting frame through a lower groove. The elastic band and the sealing sleeve are adapted to each other.

[0011] Preferably, a positioning port is provided at the free end of the placement plate body, and a positioning column is fixedly connected to the bottom of the placement plate body. A side connecting plate is fixedly connected inside the loading box body, and the positioning column can be lapped inside the side connecting plate.

[0012] Preferably, a bending portion is fixedly connected inside the loading box body, and both sides of the connecting spring are connected to the bending portion. The bending portion is used to drive the connecting spring to move, and connecting bands are uniformly fixedly connected to both sides of the loading box body.

[0013] In summary, the technical effects and advantages of the present utility model:

[0014] 1. The structure of the utility model is reasonable. By setting a sliding partition board body, before its use, the partition board body can be pulled independently according to the specific usage quantity of different types of reagents, so that the partition board body slides along the adjustment opening under force, thereby effectively adjusting the position of the partition board body independently. The internal spaces of the two cavities formed by the partition board body dividing the loading box body can be adjusted independently, so as to meet the storage requirements of different quantities of reagents. The same type of reagent can be placed inside the same cavity, and the position of the reagent is limited by the placement board body, so that it can be effectively stored. Thus, two different reagents can be stored in the same transfer box, simplifying the overall storage and use steps and making the operation more convenient.

[0015] 2. In the utility model, by setting connection buckles, before its use, the existing placement board body can be taken out and then the partition board body is pulled to adjust the position of the partition board body independently. After the partition board body is adjusted, according to the different space sizes inside each cavity, an appropriate number of placement board bodies are placed inside the cavity independently. Then, through the connection scraping plates at both ends of the placement board body, the placement board body is fixed to the connection buckles, thus completing the installation of the placement board body and enabling the storage of different quantities of reagents. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the following drawings are only some embodiments of the present application. For those skilled in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0017] Figure 1 It is a three-dimensional structure schematic diagram of the first perspective of a new transfection reagent loading box;

[0018] Figure 2 It is a three-dimensional cut-away structure schematic diagram of the loading box body of a new transfection reagent loading box;

[0019] Figure 3 It is a three-dimensional connection structure schematic diagram of the connecting spring in a new transfection reagent loading box;

[0020] Figure 4 It is a three-dimensional connection structure schematic diagram of the outer surface of a new transfection reagent loading box.

[0021] Reference numerals:

[0022] 1. Loading box body; 2. Loading cover body; 3. Adjusting opening; 4. Accommodating cavity; 5. Partition plate body; 6. Sealing member; 7. Placing plate body; 8. Mounting shaft; 9. Connecting spring; 10. Adjusting groove; 11. Adjusting block; 12. Sliding groove; 13. Sliding member; 14. Insertion slot; 15. Insertion pin; 16. Connecting buckle; 17. Connecting scraper; 18. Connecting frame; 19. Elastic band; 20. Sealing sleeve; 21. Positioning opening; 22. Positioning column; 23. Side connecting plate; 24. Bending portion; 25. Connecting band. Detailed implementation manner

[0023] To make the objectives, technical solutions and advantages of the present utility model clearer, the technical solutions in the present utility model will be clearly and completely described below with reference to the accompanying drawings in the present utility model. Obviously, the described embodiments are some but not all of the embodiments of the present utility model. All other embodiments obtained by those of ordinary skill in the art based on the embodiments in the present utility model without creative efforts shall fall within the protection scope of the present utility model.

[0024] In the description of the embodiments of the present utility model, it should be noted that the orientation or positional relationship indicated by the terms "upper", "lower", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the accompanying drawings, and is only for the convenience of describing the embodiments of the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the embodiments of the present utility model. In addition, the terms "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or implying relative importance.

[0025] In the description of the embodiments of the present utility model, it should be noted that unless otherwise clearly specified and limited, the terms "connected" and "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium. For those of ordinary skill in the art, the specific meanings of the above terms in the embodiments of the present utility model can be understood according to specific circumstances.

[0026] In the embodiments of the present utility model, unless otherwise clearly defined and limited, the first feature being "on" or "under" the second feature may be that the first and second features are in direct contact, or the first and second features are indirectly in contact through an intermediate medium. Moreover, the first feature being "above", "over" and "on top of" the second feature may be that the first feature is directly above or obliquely above the second feature, or merely indicates that the horizontal height of the first feature is higher than that of the second feature. The first feature being "under", "beneath" and "underneath" the second feature may be that the first feature is directly below or obliquely below the second feature, or merely indicates that the horizontal height of the first feature is less than that of the second feature.

[0027] In the description of this specification, the description with reference to terms such as "one embodiment", "some embodiments", "example", "specific example", or "some examples" means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the embodiments of the present utility model. In this specification, the schematic representations of the above terms do not refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in a suitable manner in any one or more embodiments or examples. In addition, without contradiction, those skilled in the art can combine and combine the different embodiments or examples described in this specification and the features of different embodiments or examples.

[0028] The following combines Figures 1-4 the embodiments shown to describe the technical solution of the present utility model:

[0029] It includes a loading box body 1 and a loading cover body 2. An adjustment opening 3 is provided inside the loading cover body 2, and a partition plate body 5 is slidably connected inside the adjustment opening 3. The partition plate body 5 is used to divide the loading box body 1 into two independent cavities. A receiving cavity 4 is formed between the loading box body 1 and the adjustment opening 3. A sealing member 6 is fixedly connected inside the receiving cavity 4, and the free end of the sealing member 6 is fixedly connected to the partition plate body 5. A plurality of placement plate bodies 7 are provided inside the loading box body 1, and the placement plate bodies 7 are used for fixedly installing reagents.

[0030] Before its use, according to the specific usage quantity of different types of reagents, the partition plate body 5 can be pulled independently, so that the partition plate body 5 slides along the adjustment opening 3 under force, thereby effectively adjusting the position of the partition plate body 5 independently, so that the internal spaces of the two cavities divided by the partition plate body 5 in the loading box body 1 can be adjusted independently, and then meet the storage requirements of different quantities of reagents. The same type of reagent can be placed inside the same cavity, and the position of the reagent can be limited by the placement plate body 7, so that it can be effectively stored. Thus, two different reagents can be stored in the same transfer box, simplifying the overall storage and use steps and making its operation more convenient.

[0031] An installation shaft 8 is fixedly connected inside the accommodating cavity 4, and a connecting spring 9 is arranged on the installation shaft 8. The free end of the seal 6 is wound around the circumference of the connecting spring 9.

[0032] When the partition body 5 is forced to move, the moving force of the partition body 5 will be transmitted to the seal 6, enabling the seal 6 to deform under force. Subsequently, the seal 6 moves synchronously with the partition body 5, thereby sealing the gap between the loading box body 1 and the partition body 5 to ensure the relative independence of the two cavities, and improving the stability when storing different reagents in the two cavities.

[0033] An adjustment groove 10 is provided inside the loading box body 1, and adjustment blocks 11 are fixedly connected to both sides of the partition body 5. The adjustment blocks 11 are slidably connected inside the adjustment groove 10.

[0034] When the partition body 5 is forced to move, the moving force of the partition body 5 can be effectively transmitted to the adjustment blocks 11, causing the adjustment blocks 11 to move synchronously with the partition body 5. Due to the setting position of the adjustment blocks 11, when the adjustment blocks 11 move, they can only move along the adjustment groove 10. At this time, the moving structure of the adjustment groove 10 and the adjustment blocks 11 will effectively limit the moving trajectory of the partition body 5, ensuring the stability of the loading cover body 2 during adjustment.

[0035] A sliding groove 12 is provided inside the loading box body 1, and sliding members 13 are fixedly connected to both sides of the partition body 5. Plug-in slots 14 are provided on both the loading box body 1 and the sliding members 13, and the number of plug-in slots 14 on the loading box body 1 is several. A plug-in pin 15 is inserted inside the sliding member 13.

[0036] The plug-in structure of the plug-in slots 14 and the plug-in pins 15 can effectively limit the relative position between the sliding members 13 and the loading box body 1. During the use of the partition body 5, the plug-in structure of the plug-in slots 14 and the plug-in pins 15 can ensure the stability of its own position, avoiding the possibility of the partition body 5 shifting or tilting under force, and further improving the stability effect of the two cavities.

[0037] A number of connecting buckles 16 are arranged inside the loading box body 1, and connecting scraping plates 17 are fixedly connected to both ends of the placement plate body 7. The connecting scraping plates 17 can be stuck on the connecting buckles 16.

[0038] Before its use, the placement plate body 7 can be taken out and then the partition plate body 5 can be pulled to independently adjust the position of the partition plate body 5. After the partition plate body 5 is adjusted, according to the different space sizes inside each cavity, an appropriate number of placement plate bodies 7 can be independently placed inside the cavity. Then, through the connecting scraping plates 17 at both ends of the placement plate body 7, the placement plate body 7 can be fixed to the connecting buckle 16, thus completing the installation of the placement plate body 7 and enabling the storage of different numbers of reagents.

[0039] A connecting frame 18 is clamped on the connecting buckle 16, and a plurality of sealing sleeves 20 are arranged inside the connecting frame 18 through an elastic band 19. The sealing sleeves 20 are rubber bodies with an open bottom.

[0040] When storing the reagent inside the loading box body 1, the sealing sleeve 20 can be directly pulled, so that the sealing sleeve 20 is sleeved on the top of the reagent tube body through the opening at the bottom. Thus, the sealing sleeve 20 can effectively seal the top of the reagent tube body, thereby ensuring the stability of reagent storage and avoiding the possibility of leakage.

[0041] A positioning port 21 is opened at the top of the loading cover body 2, and a positioning post 22 is fixedly connected to the bottom of the loading box body 1. The positioning post 22 can be inserted into the positioning port 21. Side connecting plates 23 are fixedly connected to both sides of the loading box body 1. A connecting band 25 is fixedly connected to the side wall of the side connecting plate 23, and a bending part 24 is integrally formed at the top of the side connecting plate 23. The bending part 24 is adapted to the connecting band 25.

[0042] During its use, if there are several loading boxes, the positioning post 22 of one loading box can be inserted into the positioning port 21 of another loading box, thereby completing the splicing operation of the two loading boxes. Then, the connecting band 25 on one loading box is pulled, so that the connecting band 25 is stretched under force and then sleeved inside the bending part 24 of another loading box, thus completing the fixation of the two loading boxes and making them more stable during use.

[0043] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A novel transfection reagent loading box, comprising a loading box body (1) with an opening at the bottom, characterized in that: Inside the loading box body (1), an adjustment opening (3) is connected through a loading cover body (2), and a receiving cavity (4) is arranged at the bottom of the adjustment opening (3). The loading cover body (2) is used to drive the adjustment opening (3) and the receiving cavity (4) to move vertically up and down. Sealing members (6) are arranged on both sides of the adjustment opening (3), and a partition plate body (5) and a mounting shaft (8) are fixedly connected inside the sealing members (6). A placement plate body (7) is slidably connected inside the sealing members (6). The placement plate body (7) can be in contact with the partition plate body (5), and the free end of the mounting shaft (8) is fixedly connected to the side wall of the placement plate body (7). A connecting spring (9) is slidably arranged at the bottom of the loading box body (1).

2. The novel transfection reagent loading cassette according to claim 1, characterized in that: An adjustment slot (10) is arranged inside the loading box body (1), and an adjustment block (11) is arranged on the adjustment slot (10). A sliding slot (12) is slidably connected inside the adjustment block (11), and the top end of the loading cover body (2) is fixedly connected to the bottom of the sliding slot (12).

3. A novel transfection reagent loading cassette according to claim 2, characterized in that: A number of sliding members (13) are arranged on both sides of the loading box body (1), the adjustment slot (10), and the sliding slot (12). A number of insertion slots (14) are connected inside the loading box body (1) through a number of insertion pins (15). The insertion slots (14) can be inserted inside the sliding members (13), and a connecting buckle (16) is arranged on the circumference of the insertion slots (14). The free end of the insertion pin (15) is fixedly connected to the connecting buckle (16).

4. A novel transfection reagent loading cassette according to claim 3, wherein: A connecting scraper (17) is fixedly connected to the bottom of the adjustment opening (3), and a connecting frame (18) is arranged on the receiving cavity (4). The connecting frame (18) is inserted inside the connecting scraper (17).

5. A novel transfection reagent loading cassette according to claim 4, characterized in that: An elastic band (19) is fixedly connected inside the connecting scraper (17), and a sealing sleeve (20) is fixedly connected to the top of the connecting frame (18) through a lower groove. The elastic band (19) and the sealing sleeve (20) are adapted to each other.

6. A novel transfection reagent loading cassette according to claim 5, characterized in that: A positioning opening (21) is arranged at the free end of the placement plate body (7), and a positioning column (22) is fixedly connected to the bottom of the placement plate body (7). A side connecting plate (23) is fixedly connected inside the loading box body (1), and the positioning column (22) can be lapped inside the side connecting plate (23).

7. A novel transfection reagent loading cassette according to claim 6, characterized in that: A bending part (24) is fixedly connected inside the loading box body (1), and both sides of the connecting spring (9) are connected to the bending part (24). The bending part (24) is used to drive the connecting spring (9) to move, and connecting bands (25) are uniformly fixedly connected to both sides of the loading box body (1).

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