Direct-spread sequencing reaction universal kit with sampling function

Abstract

The utility model relates to the technical field of kits, and provides a direct sequence sequencing reaction universal kit with a sampling function, which comprises a handle, a box body is fixed at the bottom end of the handle, first stop blocks are fixed in the middle of the inner wall of the box body, and a storage structure is mounted at the top end of each first stop block. Clamping grooves are fixed to the inner walls of the bottom end of the box body, and baffles are installed on the inner walls of the clamping grooves. The refrigerating structure is arranged, the box body is opened, the shell is taken out of the box body, the shell is opened, ice blocks or ice bags are placed on the surface of the storage plate, the shell is closed and placed in the box body, the position of the shell is adjusted, and the clamping base is placed in the clamping groove. Cold air released by ice cubes in the melting process enters the box body through an air outlet, molten liquid flows into the inner wall of the bottom end of the shell through a through groove in a storage plate, the refrigeration function of the device is achieved, and the applicability of the kit is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of reagent kits, and particularly relates to a general direct amplification sequencing reaction reagent kit with a sampling function. Background Art

[0002] A general direct amplification sequencing reaction reagent kit with a sampling function is an efficient molecular biology tool, which is designed to simplify experimental operations, save time, and reduce costs. This reagent kit can directly amplify target DNA fragments from various samples (such as blood cards, blood spots, tissues, plant leaves, etc.) without nucleic acid extraction or purification, and directly perform PCR amplification on the samples, greatly simplifying the experimental process. The reagent kit usually provides a variety of specifications to meet different experimental needs and can be widely used in various scientific research and clinical diagnosis fields;

[0003] When medical staff go out to work, the reagent kit needs to be carried with them. However, during this process, many reagent products and samples in the box need to be stored refrigerated, but the current reagent kits cannot provide the corresponding environment, which is not convenient for storing reagent products for a long time. Summary of the Utility Model

[0004] The purpose of the utility model is to provide a general direct amplification sequencing reaction reagent kit with a sampling function to solve the defect that the existing general direct amplification sequencing reaction reagent kit with a sampling function is not convenient for refrigeration.

[0005] To solve the above technical problems, the utility model provides the following technical solution: A general direct amplification sequencing reaction reagent kit with a sampling function, including a handle;

[0006] The bottom end of the handle is fixed with a box body. At the middle position of the inner wall of the box body, a first stop block is fixed. At the top end of the first stop block, a storage structure is installed. At the inner wall of the bottom end of the box body, a card slot is fixed. Inside the inner wall of the card slot, a baffle is installed. Outside the baffle on the inner wall of the card slot, a mounting rack is installed. Inside the mounting rack, a test tube is installed.

[0007] Outside the mounting rack on the inner wall of the card slot, a refrigeration structure is installed. The refrigeration structure includes a card holder, a shell, a second stop block, a placement plate, and an air outlet. The outer walls of the card holder are all installed on the inner wall of the card slot. The top end of the card holder is fixed with a shell. At the middle position of the inner wall of the shell, a second stop block is fixed. At the top end of the second stop block, a placement plate is installed. At the middle position of the top end of the shell, an air outlet is fixed.

[0008] On one side of the outer wall of the box body, a buckle (10) is installed. At the bottom of the buckle (10) on the outer wall of the box body, a medical sign (11) is fixed.

[0009] Preferably, the bottom end of the housing abuts against the bottom end of the card slot. A plurality of through slots are provided inside the storage plate and are equally spaced. The air outlet is a breathable layer.

[0010] Preferably, the box body includes a first heat-insulating layer, a second heat-insulating layer, heat-insulating holes and a third heat-insulating layer. The top end of the first heat-insulating layer is installed at the bottom end of the handle. The inner wall of the first heat-insulating layer is fixed with the second heat-insulating layer. Heat-insulating holes are provided inside the second heat-insulating layer. The inner wall of the second heat-insulating layer is fixed with the third heat-insulating layer.

[0011] Preferably, the first heat-insulating layer is a metal layer, and the second heat-insulating layer is a polyester fiber layer.

[0012] Preferably, the heat-insulating holes are equally spaced, and the third heat-insulating layer is a stainless steel layer.

[0013] Preferably, the storage structure includes a housing, a mounting groove, a mounting plate and reagent bottles. The bottom end of the housing is installed at the top end of the first stopper. One side of the inner bottom wall of the housing is fixed with a mounting groove. The top of the mounting groove on the inner wall of the housing is fixed with a mounting plate. Reagent bottles are installed inside the mounting plate.

[0014] Preferably, a plurality of grooves are provided inside the mounting groove and are equally spaced. The reagent bottles extend outside the mounting plate and abut against the grooves of the mounting groove. The reagent bottles are equally spaced.

[0015] The universal kit for direct expansion sequencing reaction with sampling function provided by the present utility model has the following advantages:

[0016] By providing a refrigeration structure, open the box body, take the housing out of the box body, open the housing, place ice cubes or ice packs on the surface of the storage plate, close the housing and place it inside the box body, adjust the position of the housing so that the card holder is placed inside the card slot. The cold air released during the melting process of the ice cubes enters the inside of the box body through the air outlet, and the melted liquid flows into the inner wall of the bottom end of the housing through the through slots inside the storage plate, realizing the refrigeration function of the device and improving the applicability of the kit;

[0017] By providing a box body, the first heat-insulating layer has the characteristics of heat preservation, strong stability and high temperature resistance. The second heat-insulating layer is heat-insulating cotton, which has good flexibility and heat-insulating performance. The heat-insulating holes improve the heat-insulating effect of the second heat-insulating layer. The third heat-insulating layer has the characteristics of corrosion resistance, easy cleaning, heat preservation, etc., and has good stability, realizing the heat-insulating function of the device and improving the heat-insulating property of the kit;

[0018] By providing a storage structure, opening the box body, pulling the outer shell, placing the outer shell outside the box body, placing the reagent bottle outside the mounting plate, other medical tools such as medical cotton swabs can be placed on the side of the outer shell away from the mounting plate, adjusting the position of the outer shell so that the bottom end of the outer shell abuts against the top end of the first stopper, realizing the function of convenient access of the device and improving the convenience of the reagent kit. Description of the Drawings

[0019] Figure 1 It is a front view sectional schematic diagram of the present utility model;

[0020] Figure 2 It is a side view sectional schematic diagram of the present utility model;

[0021] Figure 3 It is a top view sectional schematic diagram of the present utility model;

[0022] Figure 4 It is a front view explosion schematic diagram of the present utility model;

[0023] Figure 5 It is a three-dimensional sectional schematic diagram of the refrigeration structure of the present utility model

[0024] Figure 6 It is a three-dimensional schematic diagram of the present utility model.

[0025] Explanation of the reference numerals in the drawings: 1. Handle; 2. Box body; 201. First heat-insulating layer; 202. Second heat-insulating layer; 203. Heat-insulating hole; 204. Third heat-insulating layer; 3. First stopper; 4. Storage structure; 401. Outer shell; 402. Installation groove; 403. Mounting plate; 404. Reagent bottle; 5. Card slot; 6. Baffle; 7. Mounting rack; 8. Test tube; 9. Refrigeration structure; 901. Card holder; 902. Shell; 903. Second stopper; 904. Placing plate; 905. Air outlet; 10. Buckle; 11. Medical sign. Detailed Embodiments

[0026] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present utility model without creative efforts shall fall within the protection scope of the present utility model.

[0027] Please refer to Figures 1-6 , the direct amplification sequencing reaction general reagent kit with sampling function provided by the present utility model includes a handle 1.

[0028] Refer to Figure 1 and Figure 6As shown in the figure, a box body 2 is fixed at the bottom end of the handle 1. The box body 2 includes a first heat-insulating layer 201, a second heat-insulating layer 202, heat-insulating holes 203 and a third heat-insulating layer 204. The top end of the first heat-insulating layer 201 is installed at the bottom end of the handle 1. The inner wall of the first heat-insulating layer 201 is fixed with the second heat-insulating layer 202. The heat-insulating holes 203 are arranged inside the second heat-insulating layer 202. The inner wall of the second heat-insulating layer 202 is fixed with the third heat-insulating layer 204. The first heat-insulating layer 201 is a metal layer, the second heat-insulating layer 202 is a polyester fiber layer, the heat-insulating holes 203 are evenly distributed, and the third heat-insulating layer 204 is a stainless steel layer.

[0029] The first heat-insulating layer 201 has the characteristics of heat preservation, strong stability and high temperature resistance. The second heat-insulating layer 202 is heat-insulating cotton, which has good flexibility and heat-insulating performance. The heat-insulating holes 203 improve the heat-insulating effect of the second heat-insulating layer 202. The third heat-insulating layer 204 has the characteristics of corrosion resistance, easy cleaning, heat preservation, etc., and has good stability. Close the third heat-insulating layer 204 and fasten the buckle 10 to make the air inside and outside the first heat-insulating layer 201 not flow, resulting in a sealed state.

[0030] Refer to Figure 2 and Figure 4 As shown in the figure, first stoppers 3 are fixedly arranged at the middle positions of the inner walls of the box body 2. A storage structure 4 is installed at the top ends of the first stoppers 3. The storage structure 4 includes a housing 401, a mounting groove 402, a mounting plate 403 and reagent bottles 404. The bottom end of the housing 401 is installed at the top end of the first stopper 3. One side of the inner wall at the bottom end of the housing 401 is fixed with the mounting groove 402. The mounting plate 403 is fixed at the top of the mounting groove 402 on the inner wall of the housing 401. The reagent bottles 404 are installed inside the mounting plate 403. A plurality of grooves are arranged inside the mounting groove 402 and are evenly distributed. The reagent bottles 404 extend outside the mounting plate 403 and are in contact with the grooves of the mounting groove 402. The reagent bottles 404 are evenly distributed. Clamping grooves 5 are fixedly arranged on the inner walls of the bottom ends of the box body 2. A baffle 6 is installed on the inner wall of the clamping groove 5. An installation rack 7 is installed outside the baffle 6 on the inner wall of the clamping groove 5. Test tubes 8 are installed inside the installation rack 7.

[0031] Open the box body 2, pull the housing 401, place the housing 401 outside the box body 2, place the reagent bottles 404 outside the mounting plate 403. Other medical tools such as medical cotton swabs can be placed on the side of the housing 401 away from the mounting plate 403. Adjust the position of the housing 401 so that the bottom end of the housing 401 is in contact with the top end of the first stopper 3. The reagent bottles 404 penetrate through the mounting plate 403 and are in contact with the mounting groove 402, so that the reagent bottles 404 are stable inside the box body 2, and the reagent bottles 404 will not be damaged due to shaking when moving the box body 2.

[0032] Refer to Figure 1 and Figure 5As shown in the figure, a refrigeration structure 9 is installed outside the mounting rack 7 on the inner wall of the card slot 5. The refrigeration structure 9 includes a card seat 901, a housing 902, a second stopper 903, a storage plate 904 and an air outlet 905. The outer walls of the card seat 901 are all installed on the inner wall of the card slot 5. The top of the card seat 901 is fixed with a housing 902. The middle positions on the inner walls of the housing 902 are all fixed with second stoppers 903. The top of the second stopper 903 is installed with a storage plate 904. The middle position at the top of the housing 902 is fixed with an air outlet 905. The bottom end of the housing 902 abuts against the bottom end of the card slot 5. A plurality of through grooves are arranged inside the storage plate 904 and are equally spaced. The air outlet 905 is a breathable layer. On one side of the outer wall of the box body 2, a buckle 10 is installed. At the bottom of the buckle 10 on the outer wall of the box body 2, a medical sign 11 is fixed.

[0033] Take the housing 902 out of the box body 2, open the housing 902, place ice cubes or ice packs on the surface of the storage plate 904, close the housing 902 and place it inside the box body 2, adjust the position of the housing 902 so that the card seat 901 is placed inside the card slot 5. The cold air released by the ice cubes during the melting process enters the inside of the box body 2 through the air outlet 905. The melted liquid flows into the inner wall at the bottom end of the housing 902 through the through grooves inside the storage plate 904. When the ice cubes inside the housing 902 are completely melted, take it out of the box body 2, open the cover plate of the housing 902, take out the storage plate 904 and pour out the melted liquid.

[0034] Although the present invention has been described in detail with reference to the foregoing embodiments, for those skilled in the art, they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A universal kit for direct amplification sequencing reaction with a sampling function, comprising a handle (1); Features: A box body (2) is fixed to the bottom end of the handle (1), a first stopper (3) is fixed at the middle position of the inner wall of the box body (2), a storage structure (4) is installed on the top of the first stopper (3), a card slot (5) is fixed to the inner wall of the bottom end of the box body (2), a baffle (6) is installed on the inner wall of the card slot (5), a mounting frame (7) is installed outside the baffle (6) on the inner wall of the card slot (5), and a test tube (8) is installed inside the mounting frame (7); A refrigeration structure (9) is installed on the outside of the inner wall mounting frame (7) of the card slot (5), and the refrigeration structure (9) comprises a card seat (901), a shell (902), a second stopper (903), a storage plate (904) and an air outlet (905); the outer wall of the card seat (901) is mounted on the inner wall of the card slot (5); the shell (902) is fixed to the top of the card seat (901); the second stopper (903) is fixed at the middle position of the inner wall of the shell (902); the storage plate (904) is installed at the top of the second stopper (903); and the air outlet (905) is fixed at the middle position of the top of the shell (902); A buckle (10) is installed on one side of the outer wall of the box body (2), and a medical mark (11) is fixed on the bottom of the buckle (10) on the outer wall of the box body (2).

2. The general kit for direct amplification sequencing reaction with sampling function according to claim 1, wherein: The bottom end of the shell (902) contacts the bottom end of the card slot (5); a plurality of through slots are arranged inside the storage plate (904) and are distributed at equal intervals; and the air outlet (905) is a breathable layer.

3. The general kit for direct expansion sequencing reaction with sampling function according to claim 1, characterized in that: The box body (2) comprises a first thermal insulation layer (201), a second thermal insulation layer (202), a thermal insulation hole (203) and a third thermal insulation layer (204); the top end of the first thermal insulation layer (201) is mounted on the bottom end of the handle (1); the second thermal insulation layer (202) is fixed to the inner wall of the first thermal insulation layer (201); the thermal insulation hole (203) is arranged inside the second thermal insulation layer (202); and the third thermal insulation layer (204) is fixed to the inner wall of the second thermal insulation layer (202).

4. The general kit for direct expansion sequencing reaction with sampling function according to claim 3, wherein: The first thermal insulation layer (201) is a metal layer, and the second thermal insulation layer (202) is a polyester fiber layer.

5. The general kit for direct expansion sequencing reaction with sampling function according to claim 3, wherein: The insulation holes (203) are distributed at equal intervals, and the third insulation layer (204) is a stainless steel layer.

6. The general kit for direct expansion sequencing reaction with sampling function according to claim 1, characterized in that: The storage structure (4) comprises a shell (401), a mounting groove (402), a mounting plate (403) and a reagent bottle (404); the bottom end of the shell (401) is mounted on the top end of the first stopper (3); a mounting groove (402) is fixed to one side of the inner wall of the bottom end of the shell (401); a mounting plate (403) is fixed to the top of the mounting groove (402) on the inner wall of the shell (401); and reagent bottles (404) are mounted inside the mounting plate (403).

7. The general kit for direct expansion sequencing reaction with sampling function according to claim 6, characterized in that: A plurality of grooves are provided inside the installation groove (402) and are equally spaced. The reagent bottle (404) extends to the outside of the installation plate (403) and abuts against the grooves of the installation groove (402). The reagent bottles (404) are equally spaced.

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