Full-automatic solid-phase microextraction device
Through the fully automatic solid-phase microextraction device, the automatic processing of the cotton swab solid-phase microextraction head is realized, which solves the problems of cumbersome operation and blockage, reduces costs and improves processing efficiency.
Patent Information
- Application Number
- CN202421501983.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Priority Date
- 2023-08-11
- Filing Date
- 2024-06-28
- Publication Date
- 2025-07-22
- Estimated Expiration
- 2034-06-28
AI Technical Summary
The prior art lacks fully automatic pretreatment equipment suitable for solid-phase extraction heads of cotton swabs. The traditional solid-phase extraction method is cumbersome and costly, and is prone to clogging and elution for viscous and colloidal complex samples.
A fully automatic solid-phase micro-extraction device is designed, and the semi-automated treatment of adsorption, cleaning and desorption of the solid-phase micro-extraction head of the cotton swab is achieved by oscillating stirring, including a vibrating stirring mechanism and a probe, and automated operation is achieved through the controller.
It improves processing efficiency, simplifies operating procedures, reduces costs, overcomes the problems of blockage and elution difficulties, and the cost is only one-tenth of that of traditional fully automatic solid-phase extractors.
Smart Images
Figure CN223139116U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the field of solid-phase microextraction, in particular to a full-automatic solid-phase microextraction device. Background Art
[0002] Solid-phase microextraction (SPME) technology is one of the commonly used techniques for sample pretreatment in laboratories.
[0003] The traditional solid-phase extraction method for pretreatment has the following disadvantages: ① The column needs to be pre-washed with methanol and water → the extract is passed through the column → the impurities are washed with water → elution and other steps, and the operation is relatively complicated; ② The cost of the solid-phase extraction column is relatively high; ③ Automation can be achieved through a full-automatic solid-phase extraction instrument, but the price of the full-automatic solid-phase extraction instrument is expensive, and it is difficult for general laboratories to be equipped; ④ There are problems such as easy blockage and difficult elution for viscous, colloidal and complex samples.
[0004] Previous research used cotton swabs, which are commonly seen in people's daily lives, as carriers, and replaced the traditional solid-phase extraction column with a solid-phase microextraction rod made by wrapping fillers on the cotton swab. A cotton swab SPME solid-phase microextraction head covered with a PANB coating was prepared by a simple "dip-coating" method to achieve the detection of more than a hundred kinds of agricultural and veterinary residues and mycotoxins. The cost is low, the operation is simple, and the detection results are accurate and reliable; however, there is no full-automatic pretreatment equipment suitable for the cotton swab solid-phase extraction head so far.
[0005] To solve the above problems, the utility model proposes a full-automatic solid-phase microextraction device, which is an automatic processing equipment suitable for the adsorption, cleaning and desorption of the cotton swab SPME solid-phase microextraction head. Summary of the Utility Model
[0006] The purpose of the utility model is to provide a full-automatic solid-phase microextraction device to solve the problem that there is no full-automatic pretreatment equipment suitable for the cotton swab solid-phase extraction head at present.
[0007] To achieve the above purpose, the utility model provides the following technical solutions:
[0008] A full-automatic solid-phase microextraction device includes a base. One side above the base is provided with a controller, and one side of the controller is provided with a moving device. Below the moving device, a vibration stirring mechanism is arranged, and below the vibration stirring mechanism is a probe, and the probe is connected to a probe cotton swab. On the other side above the base, a test tube tray is arranged.
[0009] Preferably, the moving device is a left and right automatic telescopic device, and the left and right automatic telescopic device is arranged on one side of the controller.
[0010] Preferably, the vibration stirring mechanism is an up-and-down moving and rotating mechanism, which includes a rotating motor, a rotating synchronous belt, a lifting motor, a lifting screw, a lifting rotating shaft, a sleeve rod, a synchronous rotating sleeve, a first support plate, a second support plate, and a third support plate. The rotating motor is installed on the first support plate through a bracket, and the lifting motor is installed on the first support plate. One end of the lifting screw is connected to the lifting motor, and the other end of the lifting screw is sleeved on one end of the second support plate. One end of the lifting rotating shaft is sleeved on the other end of the second support plate, and the other end of the lifting rotating shaft sequentially passes through the sleeve rod, the third support plate, the synchronous rotating sleeve, the first support plate, and the sleeve rod and then is connected to the probe. The rotating synchronous belt is sleeved on the rotating shaft of the rotating motor, the synchronous rotating sleeve, and the supporting runner.
[0011] Preferably, the controller is a touch screen, and the controller is electrically connected to the left and right automatic telescopic fixing and vibration stirring mechanisms. All control functions and parameter settings are realized on the touch screen.
[0012] Preferably, the test tube tray is fixed on the base. The test tube tray is a multi-hole centrifuge tube rack, which is adapted to 0.5 ml, 1.5 ml, and 2.0 ml test tubes.
[0013] Preferably, the probe includes a probe tube, a probe jaw, and a probe socket threadedly connected to the probe tube. The top of the probe tube is sleeved on the bottom of the lifting rotating shaft and fixed by screws. The bottom of the probe tube is sleeved on one end of the probe jaw without a jaw head. When one end of the probe socket is screwed and fixed on the outer side of the lower part of the probe tube, the jaw head of the probe jaw fits against the conical part of the probe socket, and the conical part of the probe socket gives the jaw head of the probe jaw an inward clamping force, thereby realizing the fixation of the top of the probe cotton swab.
[0014] Preferably, the number of the lifting rotating shafts and the probes is 5 - 8. The probes are automatically adapted to the test tubes at various positions on the test tube tray through the left and right automatic telescopic fixing.
[0015] Preferably, the length, width, and height of the fully automatic solid-phase microextraction device are 500X260X300 mm.
[0016] The beneficial effects of the present utility model: The fully automatic solid-phase microextraction instrument of the present utility model realizes the semi-automatic processing of the large-scale adsorption, cleaning, and desorption of the solid-phase microextraction head of the cotton swab, greatly improving the processing efficiency and eliminating the need to purchase expensive fully automatic solid-phase extraction instruments; The method of using vibration stirring (stirring while moving up and down) to replace column passing overcomes the problems of easy blockage and poor elution for viscous and colloidal complex samples; It overcomes the problems of poor purification effect of liquid-liquid extraction for complex sample matrices and possible interference.
[0017] The steps of solid-phase extraction column passing, such as pre-washing with methanol and water → passing the extract through the column → washing impurities with water → elution, etc., are compressed into adsorption → washing → elution by a simple shaking and stirring method. The operation is greatly simplified, and the time is shortened to one-third of the original. The cost of the full-automatic solid-phase microextraction instrument of the present invention is only one-tenth of that of the traditional full-automatic solid-phase extraction instrument. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] Figure 1 It is a front view structural schematic diagram of the present invention;
[0019] Figure 2 It is a right view structural schematic diagram of the present invention;
[0020] Figure 3 It is a structural schematic diagram of the vibration stirring mechanism in the present invention;
[0021] Figure 4 It is a structural schematic diagram of the probe jaw in the present invention.
[0022] Wherein: 1. Base, 2. Controller, 3. Probe cotton swab, 4. Test tube tray, 5. Left and right automatic telescopic fixture, 6. Rotating motor, 7. Rotating synchronous belt, 8. Lifting motor, 9. Lifting screw, 10. Lifting rotating shaft, 11. Sleeve rod, 12. Synchronous rotating sleeve, 13. First support plate, 14. Second support plate, 15. Third support plate, 16. Bracket, 17. Rotating motor rotating shaft, 18. Support wheel, 19. Probe tube, 20. Probe jaw, 20-1. Claw head, 21. Probe socket, 22. Test tube, 23. Screw. DETAILED DESCRIPTION OF THE EMBODIMENTS
[0023] To further elaborate on the technical means and effects adopted by the present invention to achieve the predetermined invention purpose, the following, in conjunction with the attached drawings and preferred embodiments, details the specific implementation manners, structures, features and their effects of the present invention as follows.
[0024] As Figure 1 shown, a full-automatic solid-phase microextraction device includes a base 1. A controller 2 is provided on one side above the base 1. A moving device is provided on one side of the controller 2. A vibration stirring mechanism is provided below the moving device. A probe is provided below the vibration stirring mechanism. The probe is connected to a probe cotton swab 3. A test tube tray 4 is provided on the other side above the base 1.
[0025] In this embodiment, the moving device is a left and right automatic telescopic fixture 5, and the left and right automatic telescopic fixture 5 is provided on one side of the controller 2.
[0026] In this embodiment, the vibration stirring mechanism is an up-and-down moving and rotating mechanism, which includes a rotating motor 6, a rotating synchronous belt 7, a lifting motor 8, a lifting screw 9, a lifting rotating shaft 10, a sleeve rod 11, a synchronous rotating sleeve 12, a first support plate 13, a second support plate 14, and a third support plate 15. The rotating motor 6 is installed on the first support plate 13 through a bracket 16, the lifting motor 8 is installed on the first support plate 13, one end of the lifting screw 9 is connected to the lifting motor 8, the other end of the lifting screw 9 is sleeved on one end of the second support plate 14, one end of the lifting rotating shaft 10 is sleeved on the other end of the second support plate 14, and the other end of the lifting rotating shaft 10 passes through the sleeve rod 11, the third support plate 15, the synchronous rotating sleeve 12, the first support plate 13, and the sleeve rod 11 in sequence and is connected to the probe. The rotating synchronous belt 7 is sleeved on the rotating shaft 17 of the rotating motor, the synchronous rotating sleeve 12, and the support runner 18.
[0027] In this embodiment, the controller 2 is a 4.3-inch touch screen. The controller 2 is electrically connected to the left and right automatic telescopic device 5 and the vibration stirring mechanism, and all control functions and parameter settings are realized on the touch screen.
[0028] In this embodiment, the test tube tray 4 is fixed on the base 1. The test tube tray 4 is a 60-hole centrifuge tube rack, with 5 tubes in a row and a total of 12 rows, suitable for 0.5ml, 1.5ml, and 2.0ml test tubes.
[0029] In this embodiment, the probe includes a probe tube 19, a probe jaw 20, and a probe socket head 21 threadedly connected to the probe tube 19. The top of the probe tube 19 is sleeved on the bottom of the lifting rotating shaft 10 and fixed by a screw 23. The bottom of the probe tube 19 is sleeved on one end of the probe jaw 20 where the jaw head 20-1 is not provided. When one end of the probe socket head 21 is screwed and fixed on the outer side of the lower part of the probe tube 19, the jaw head 20-1 of the probe jaw 20 fits against the conical part of the probe socket head 21, and the conical part of the probe socket head 21 gives an inward clamping force to the jaw head 20-1 of the probe jaw 20, thereby realizing the fixation of the top of the probe cotton swab 3.
[0030] In this embodiment, the dimensions of a fully automatic solid-phase microextraction device are 500X260X300mm in length, width, and height.
[0031] In this embodiment, both the number of the lifting rotating shafts 10 and the number of probes are 5. The probes are automatically adapted to the test tubes 22 at each position in 12 rows on the test tube tray 4 through the left and right automatic telescopic device 5.
[0032] In this embodiment, the probe cotton swab 3 realizes the up-and-down movement and simultaneous rotation of the lifting rotating shaft 10 through the lifting screw 9, the rotating motor 6, and the rotating synchronous belt 7, and the frequency is adjusted by the controller 2.
[0033] Working principle: After screwing the probe socket 21 downward from the probe tube 19 by a certain distance, the tops of the 5 probe cotton swabs 3 made by wrapping filler with cotton swabs are sequentially passed through the holes at the top of the probe socket 21 and then through the jaw heads 20-1 of the probe jaws 20. Then, screw the probe socket 21 in the reverse direction to achieve the fixed connection between the probe tube 19 and the probe socket 21. During this process, the jaw heads 20-1 of the probe jaws 20 are in contact with the conical part of the probe socket 21, and the conical part of the probe socket 21 gives an inward clamping force to the jaw heads 20-1 of the probe jaws 20, so as to fix the tops of the probe cotton swabs 3. By adjusting the control function and parameter setting through the controller 2, the left and right automatic telescopic fixer 5 drives the probe cotton swab 3 to move above a row of test tubes 22 in the selected test tube tray 4. Start the rotation motor 6 and the lifting motor 8. The rotation of the rotation motor 6 drives the rotation of the rotation synchronous belt 7. The rotation of the rotation synchronous belt 7 drives the rotation of the synchronous rotation sleeve 12 (at this time, the rotation synchronous belt 7 is cross-sleeved on multiple synchronous rotation sleeves 12 in an S shape). The rotation of the synchronous rotation sleeve 12 drives the rotation of the lifting rotation shaft 10. The lifting motor 8 drives the rotation of the lifting screw 9. The rotation of the lifting screw 9 drives the second support plate 14 to move downward. The downward movement of the second support plate 14 drives the downward movement of the lifting rotation shaft 10. The downward movement of the lifting rotation shaft 10 makes the probe cotton swab 3 probe downward into the test tube 22. The rotation of the lifting rotation shaft 10 drives the probe and the probe cotton swab 3 to rotate at the set speed. The lifting rotation shaft 10 moves up and down and rotates at the same time, driving the probe and the probe cotton swab 3 to move up and down and rotate at the same time, so as to achieve oscillation. After reaching the set time, the rotation motor 6 stops working, and the lifting rotation shaft 10 stops rotating. The lifting motor 8 controls the lifting screw 9 to rotate in the reverse direction. The reverse rotation of the lifting screw 9 drives the second support plate 14 to move upward. The upward movement of the second support plate 14 drives the upward movement of the lifting rotation shaft 10. At this time, the lifting rotation shaft 10 stops rotating and moves upward at a constant speed, driving the probe cotton swab 3 to move out of the test tube 22 above the test tube 22. Select a new row of test tubes 22 through the left and right automatic telescopic fixer 5 and repeat the above operations.
[0034] The above is only a preferred embodiment of the present invention, and does not impose any form of limitation on the present invention. Although the present invention has been disclosed above with a preferred embodiment, it is not intended to limit the present invention. Any person skilled in the art can make some changes or modifications to the above-disclosed technical content to obtain equivalent embodiments with equivalent changes, but as long as the technical content of the present invention is not departed from, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention still fall within the scope of the technical solution of the present invention.
Claims
1. An automatic solid-phase microextraction device, characterized in that: It includes a base, on one side above the base is provided a controller, on one side of the controller is provided a moving device, below the moving device is provided a vibration stirring mechanism, below the vibration stirring mechanism is provided a probe, the probe is connected to a probe cotton swab, on the other side above the base is provided a test tube tray, the probe includes a probe tube, a probe jaw, and a probe socket head threadedly connected to the probe tube, the top of the probe tube is sleeved at the bottom of a lifting and rotating shaft and fixed by screws, the bottom of the probe tube is sleeved at one end of the probe jaw without a jaw head, when one end of the probe socket head is screwed and fixed on the outer side of the lower part of the probe tube, the jaw head of the probe jaw fits with the conical part of the probe socket head, and the conical part of the probe socket head gives an inward clamping force to the jaw head of the probe jaw, so as to realize the fixation of the top of the probe cotton swab.
2. The fully automatic solid phase microextraction device according to claim 1, characterized in that: The moving device is a left and right automatic telescopic device, and the left and right automatic telescopic device is arranged on one side of the controller.
3. The fully automatic solid phase microextraction device according to claim 1, characterized in that: The vibration stirring mechanism is an up and down moving and rotating mechanism, including a rotating motor, a rotating synchronous belt, a lifting motor, a lifting screw, a lifting and rotating shaft, a sleeve rod, a synchronous rotating sleeve, a first support plate, a second support plate, and a third support plate, the rotating motor is installed on the first support plate through a bracket, the lifting motor is installed on the first support plate, one end of the lifting screw is connected to the lifting motor, the other end of the lifting screw is sleeved at one end of the second support plate, one end of the lifting and rotating shaft is sleeved at the other end of the second support plate, the other end of the lifting and rotating shaft sequentially passes through the sleeve rod, the third support plate, the synchronous rotating sleeve, the first support plate, the sleeve rod and then is connected to the probe, the rotating synchronous belt is sleeved on the rotating shaft of the rotating motor, the synchronous rotating sleeve, and the support wheel.
4. The fully automatic solid-phase microextraction device according to claim 1, characterized in that: The controller is a touch screen, and the controller is electrically connected to the left and right automatic telescopic device and the vibration stirring mechanism, and all control functions and parameter settings are realized in the touch screen.
5. The fully automatic solid-phase microextraction device according to claim 1, characterized in that: The test tube tray is fixed on the base, and the test tube tray is a multi-hole centrifuge tube rack, which is adapted to 0.5ml, 1.5ml, and 2.0ml test tubes.
6. The fully automatic solid phase microextraction device according to claim 3, characterized in that: The number of the lifting and rotating shafts and the probes is 5 - 8, and the probes automatically adapt to the test tubes at each position on the test tube tray through the left and right automatic telescopic device.
7. The fully automatic solid phase microextraction device according to claim 1, wherein: The dimensions of the fully automatic solid phase microextraction device in length, width, and height are 500X260X300mm.