Adjusting device for microbiological detection light path

Abstract

The utility model provides an adjusting device for a microbiological detection light path, which comprises a laser, a flow chamber, a scattered light detector and a fluorescence detector, and a light spot shaping system, a scattered light collecting system and a fluorescence capturing system are respectively arranged between the flow chamber and the laser, between the flow chamber and the scattered light detector and between the flow chamber and the fluorescence detector. The flow chamber is arranged in the central block, the laser is arranged on the laser mounting seat, a first adjusting structure capable of adjusting the position of the light spot shaping system is arranged between the central block and the laser mounting seat, and a second adjusting structure capable of adjusting the positions of the scattered light detector and the scattered light collecting system is arranged between the scattered light detector and the central block. And a third adjusting structure capable of adjusting the positions of the fluorescence detector and the fluorescence capturing system is arranged between the fluorescence detector and the central block. The optical path structure is high in concentricity, convenient to adjust and high in overall optical path performance, and the optical path structure can be adjusted according to needs.

Description

Technical Field

[0001] The utility model relates to the technical field of microorganism detection, in particular to an adjusting device for the optical path of microorganism detection. Background Technique

[0002] Microorganisms in the water system contain riboflavin, tryptophan, reductase, etc. After being irradiated by ultraviolet light, these substances will emit fluorescence. These substances also have their specific fluorescence emission spectra. For example, the emission spectrum of riboflavin is mainly distributed in the range of 500-620nm. Since different microorganisms contain different components, different microorganisms have different fluorescence characteristics.

[0003] The principle of laser-induced fluorescence technology is to use short-pulse lasers with good monochromaticity, short wavelengths, and high energy to excite certain molecules or atoms, and measure the fluorescence emitted when returning from the excited state to the ground state, so as to achieve the measurement of the concentration of atoms and molecules, the detection of energy transfer within molecules, etc. The principle of Mie scattering is that when the particle diameter is comparable to the wavelength of the radiation, light scattering will occur, and the scattering in the forward direction of the light is stronger than that in the backward direction. The principle of Mie scattering can be used to measure the size of particles.

[0004] In the prior patent of the applicant, a detection optical path for detecting microorganisms using the principles of laser-induced fluorescence technology and Mie scattering is mentioned. In the design of this optical path, due to differences in optical devices and structural components, it is impossible to ensure that the optical paths assembled by each processing are consistent. To solve such problems, generally, the core dimensions need to be adjusted to ensure that the performance of each optical path reaches the most ideal state. Content of the Utility Model

[0005] In order to solve the problems of the above-mentioned prior art, the utility model provides an adjusting device for the optical path of microorganism detection, which has high concentricity, high adjustment accuracy of the optical path adjustment components, and is convenient to adjust.

[0006] The adopted technical solution is as follows:

[0007] An adjusting device for the optical path of microorganism detection includes a laser, a flow cell, a scattered light detector, and a fluorescence detector. A spot shaping system, a scattered light collection system, and a fluorescence capture system are respectively arranged between the flow cell and the laser, the scattered light detector, and the fluorescence detector. The flow cell is arranged in a central block, and the laser is arranged on a laser mounting seat. A first adjusting structure for adjusting the position of the spot shaping system is arranged between the central block and the laser mounting seat. A second adjusting structure for adjusting the positions of the scattered light detector and the scattered light collection system is arranged between the scattered light detector and the central block. A third adjusting structure for adjusting the positions of the fluorescence detector and the fluorescence capture system is arranged between the fluorescence detector and the central block.

[0008] Further, the first adjustment structure includes a spot adjustment knob concentrically installed with the laser mounting base and a spot adjustment block for installing the spot shaping system.

[0009] Further, the spot adjustment knob is threadedly connected to the spot adjustment block, and the spot adjustment knob is rotatably connected to the laser mounting base.

[0010] Further, a first sliding groove for the spot adjustment block to slide is provided in the center block, and a first limiting section surface for restricting the rotation of the spot adjustment block is provided at the mating part of the spot adjustment block and the first sliding groove.

[0011] Further, the second adjustment structure includes a front diffusing lens adjustment block for installing the scattered light collection system and a mounting plate for installing the scattered light detector connected to the front diffusing lens adjustment block, and the front diffusing lens adjustment block is concentrically installed with the center block.

[0012] Further, the front diffusing lens adjustment block is threadedly connected to the center block, a second sliding groove for the front diffusing lens adjustment block to slide is provided in the center block, and the second sliding groove is provided with a taper angle at one end close to the flow chamber.

[0013] Further, the third adjustment structure includes a fluorescence adjustment block for installing the fluorescence capture system and a fluorescence mounting base for installing the fluorescence detector, the fluorescence detector is fixedly connected to the fluorescence mounting base, and the fluorescence adjustment block is concentrically installed with the center block.

[0014] Further, the third adjustment structure further includes an adjustment nut provided between the center block and the fluorescence adjustment block.

[0015] Further, a third sliding groove for the fluorescence adjustment block to slide is provided in the center block, a second limiting section surface for restricting the rotation of the fluorescence adjustment block is provided at the mating part of the fluorescence adjustment block and the third sliding groove, and the third sliding groove is provided with a taper angle at one end close to the flow chamber.

[0016] Further, a plano-concave spherical mirror is provided on the side of the flow chamber opposite to the fluorescence capture system, a fourth sliding groove is provided in the center block in the direction towards the plano-concave spherical mirror, and the fourth sliding groove is provided with a taper angle at one end close to the flow chamber.

[0017] Compared with the prior art, the beneficial effects produced by the present utility model are:

[0018] The utility model provides an adjusting device for the optical path of microorganism detection, which includes a flow chamber arranged on a central block. The whole is light-shielded without the interference of external light. The detection devices arranged at various angles of the central block are concentrically installed with high concentricity. At the same time, it is adjustable, and the adjusting precision of the optical path adjusting component is high. The adjustment is convenient, and the movement of the component can be controlled through an external rotation structure. Conical angles are adopted at various angles towards which the central block faces, that is, the design that the bottom hole of the conical angle ≤ the width of the flow chamber effectively avoids the continuous reflection of the original laser inside, thereby reducing the background signal. The structure of the utility model is compact and the cost is low. Description of the Drawings

[0019] Figure 1 is the overall sectional view of the utility model;

[0020] Figure 2 is the three-dimensional view of the utility model;

[0021] Figure 3 is the side view of the utility model;

[0022] Figure 4 is Figure 3 the sectional view in the direction of B-B;

[0023] Figure 5 is the partial structure split view of the utility model;

[0024] Among them, laser 1, flow chamber 2, scattered light detector 3, fluorescence detector 4, spot shaping system 5, first plano-convex cylindrical lens 501, second plano-convex cylindrical lens 502, scattered light collection system 6, scattered light plano-convex lens 601, scattered light aspherical lens 602, front scattered light baffle 603, fluorescence capture system 7, fluorescence aspherical lens 701, dichroic mirror 702, filter 703, fluorescence plano-convex lens 704, extinction baffle 705, central block 8, laser mounting seat 9, first adjustment structure 10, spot adjustment knob 1001, spot adjustment block 1002, second adjustment structure 11, front scattered lens adjustment block 1101, mounting plate 1102, third adjustment structure 12, fluorescence adjustment block 1201, adjustment nut 1202, fluorescence mounting seat 13, fourth chute 14, first chute 15, first limiting section 16, second chute 17, third chute 18, second limiting section 19, plano-concave spherical mirror 20, laser fixing plate 21. Detailed Embodiments

[0025] The following further describes the utility model in conjunction with specific embodiments.

[0026] Refer to Figures 1-5, An adjustment device for a microbial detection optical path, comprising a laser 1, a flow cell 2, a scattered light detector 3, and a fluorescence detector 4. A spot shaping system 5, a scattered light collection system 6, and a fluorescence capture system 7 are respectively provided between the flow cell 2 and the laser 1, the scattered light detector 3, and the fluorescence detector 4. The flow cell 2 is disposed within a central block 8, and the laser 1 is mounted on a laser mounting base 9. A first adjustment structure 10 for adjusting the position of the spot shaping system 5 is provided between the central block 8 and the laser mounting base 9. A second adjustment structure 11 for adjusting the positions of the scattered light detector 3 and the scattered light collection system 6 is provided between the scattered light detector 3 and the central block 8. A third adjustment structure 12 for adjusting the positions of the fluorescence detector 4 and the fluorescence capture system 7 is provided between the fluorescence detector 4 and the central block 8.

[0027] The first adjustment structure 10 includes a spot adjustment knob 1001 concentrically mounted with the laser mounting base 9 and a spot adjustment block 1002 for mounting the spot shaping system 5. The spot shaping system 5 includes a first plano-convex cylindrical lens 501 and a second plano-convex cylindrical lens 502.

[0028] The laser 1 is locked to the laser adjustment seat 9 through a laser fixing plate 21. The spot adjustment knob 1001 and the spot adjustment block 1002 are threadedly connected. The spot adjustment knob 1001 is rotatably connected to the laser mounting base 9. As Figure 1 , the spot adjustment knob 1001 is provided with a convex block that is directly inserted into the notch of the laser mounting base 9, and the laser mounting base 9 abuts against the spot adjustment knob to limit the sliding of the spot adjustment knob.

[0029] Restricted by the laser mounting base 9, the spot adjustment knob 1001 can only rotate and cannot slide. During this process, the position of the laser mounting base 9 remains unchanged. A first sliding groove 15 for the spot adjustment block 1002 to slide is provided within the central block 8. A first limiting cutting surface 16 for restricting the rotation of the spot adjustment block 1002 is provided at the mating portion of the spot adjustment block 1002 and the first sliding groove 15. Setting a limiting cutting surface instead of a circumferential surface can restrict the rotation of the spot adjustment block 1002.

[0030] Since the spot adjustment block 1002 and the spot adjustment knob 1001 are threadedly connected, the position of the spot adjustment block 1002 can be adjusted by rotating the spot adjustment knob 1001, that is, adjusting the positions of the first and second plano-convex cylindrical lenses, and the spot shaping system is adjusted to form a suitable spot size.

[0031] The second adjustment structure 11 described above includes a front diffusing lens adjustment block 1101 for mounting the scattered light collection system 6, and a mounting plate 1102 connected to the front diffusing lens adjustment block 1101 for mounting the scattered light detector 3. The front diffusing lens adjustment block 1101 is concentrically mounted with the central block 8. The scattered light collection system 6 includes a scattered light plano-convex lens 601 and a scattered light aspherical lens 602 that are sequentially mounted in a direction away from the scattered light detector 3. A front diffusing light shield 603 is provided at one end of the scattered light aspherical lens 602 close to the flow chamber 2.

[0032] A scattered light aspherical lens 602 for large-angle scattered light condensation is provided in the front diffusing lens adjustment block 1101. A front diffusing light shield 603 is provided at one end of the scattered light aspherical lens 602 close to the flow chamber 2, which is used to absorb the light that directly enters the scattered light aspherical lens 602 for scattered light condensation when the laser spot passes through the detection area without particles, thereby weakening the intensity of the background signal. A scattered light detector 3 is provided at the focal point of the scattered light plano-convex lens 601 for signal reception.

[0033] The front diffusing lens adjustment block 1101 is threadedly connected to the central block 8, and the scattered light reception angle is adjusted by rotating the front diffusing lens adjustment block 1101. The central block 8 is provided with a second sliding groove 17 for the front diffusing lens adjustment block 1101 to slide. The second sliding groove 17 is provided with a taper angle at one end close to the flow chamber. The taper angle is set to prevent light from other directions from entering and causing an increase in the background signal.

[0034] The third adjustment structure 12 includes a fluorescence adjustment block 1201 for mounting the fluorescence capture system 7, a fluorescence mounting base 13 for mounting the fluorescence detector 4, and an adjustment nut 1202 provided between the central block 8 and the fluorescence adjustment block 1201. The fluorescence adjustment block 1201 is fixedly connected to the fluorescence mounting base 13. The fluorescence capture system 7 includes a fluorescence aspherical lens 701, a dichroic mirror 702 (transmitting fluorescence and reflecting the original wavelength light of the laser), a filter 703 (filtering stray light), and a fluorescence plano-convex lens 704 (focusing and collecting light) that are sequentially arranged in a direction away from the flow chamber. The fluorescence adjustment block 1201 is concentrically mounted with the central block 8.

[0035] Further, an extinction baffle 705 is provided on the side of the main hole where the dichroic mirror 702 is placed, to prevent the reflected light of the dichroic mirror 702 from being reflected multiple times and causing fluctuations in the original fluorescence reception signal.

[0036] The adjusting nut 1202 is threadedly connected to the fluorescence adjusting block. A third chute 18 for the fluorescence adjusting block 1201 to slide is provided in the central block 8. A second limiting section 19 for restricting the rotation of the fluorescence adjusting block 1201 is provided at the mating part of the fluorescence adjusting block 1201 and the third chute 18. The section is provided instead of a circumferential surface to prevent the fluorescence adjusting block 1201 from rotating. In order to adjust the position of the fluorescence adjusting block, the adjusting nut 1202 can only rotate and cannot slide. Since the adjusting nut is threadedly connected to the fluorescence adjusting block and the fluorescence adjusting block is restricted from rotating, when the adjusting nut 1202 rotates, the fluorescence adjusting block 1201 can be adjusted to slide, that is, the positions of the fluorescence capture system 7 and the fluorescence detector 4 are adjusted.

[0037] A plano-concave spherical mirror 20 is provided on the side of the flow chamber 2 opposite to the fluorescence capture system 7. A fourth chute 14 is provided in the central block 8 facing the plano-concave spherical mirror 20. The end of the fourth chute 14 close to the flow chamber is provided with a taper angle. The taper angle is used to prevent light from entering from other directions and causing an increase in the background signal, and is used to increase the fluorescence receiving angle.

[0038] Each adjusting block of the present utility model is concentrically installed, with high concentricity, accurate signal reception, high intensity, adjustable positions of each structure, high adjustment accuracy of the optical path adjusting components, and the spot size and receiving angle can be adjusted according to actual needs, resulting in better detection effects. At the same time, the operation is convenient, and the adjustment can be achieved by rotating the external rotating structure. The second, third, and fourth chutes provided in the central block are all provided with taper angles to weaken the background signal.

[0039] The above are only optional embodiments of the present utility model, and do not limit the patent scope of the present utility model accordingly. Any equivalent structural transformation made under the inventive concept of the present utility model by using the content of the specification and drawings of the present utility model, or any direct / indirect application in other related technical fields, is included in the patent protection scope of the present utility model.

Claims

1. An adjustment device for a light path of microorganism detection, comprising a laser (1), a flow cell (2), a scattered light detector (3) and a fluorescence detector (4). A spot shaping system (5), a scattered light collection system (6) and a fluorescence capture system (7) are respectively arranged between the flow cell (2) and the laser (1), the scattered light detector (3) and the fluorescence detector (4). It is characterized in that: The flow cell (2) is arranged inside the central block (8), and the laser (1) is arranged on the laser mounting base (9). A first adjusting structure (10) for adjusting the position of the spot shaping system (5) is provided between the central block (8) and the laser mounting base (9). A second adjusting structure (11) for adjusting the positions of the scattered light detector (3) and the scattered light collection system (6) is provided between the scattered light detector (3) and the central block (8). A third adjusting structure (12) for adjusting the positions of the fluorescence detector (4) and the fluorescence capture system (7) is provided between the fluorescence detector (4) and the central block (8).

2. The adjusting device for the optical path of microorganism detection according to claim 1, characterized in that: The first adjusting structure (10) includes a spot adjusting knob (1001) concentrically mounted with the laser mounting base (9) and a spot adjusting block (1002) for mounting the spot shaping system (5).

3. The adjusting device for the optical path of microorganism detection according to claim 2, wherein: The spot adjusting knob (1001) is threadedly connected to the spot adjusting block (1002), and the spot adjusting knob (1001) is rotatably connected to the laser mounting base (9).

4. The adjusting device for the optical path of microorganism detection according to claim 2, characterized in that: A first sliding groove (15) for the spot adjusting block (1002) to slide is provided inside the central block (8). A first limiting section (16) for restricting the rotation of the spot adjusting block (1002) is provided at the mating part of the spot adjusting block (1002) and the first sliding groove (15).

5. The adjusting device for the optical path of microorganism detection according to claim 1, characterized in that: The second adjusting structure (11) includes a front diffusing lens adjusting block (1101) for mounting the scattered light collection system (6) and a mounting plate (1102) connected to the front diffusing lens adjusting block (1101) for mounting the scattered light detector (3). The front diffusing lens adjusting block (1101) is concentrically mounted with the central block (8).

6. The adjusting device for the optical path of microorganism detection according to claim 5, characterized in that: The front diffusing lens adjusting block (1101) is threadedly connected to the central block (8). A second sliding groove (17) for the front diffusing lens adjusting block (1101) to slide is provided inside the central block (8). The second sliding groove (17) is provided with a taper angle at the end close to the flow cell.

7. The adjusting device for the optical path of microorganism detection according to claim 1, characterized in that: The third adjusting structure (12) includes a fluorescence adjusting block (1201) for mounting the fluorescence capture system (7) and a fluorescence mounting base (13) for mounting the fluorescence detector (4). The fluorescence detector (4) is fixedly connected to the fluorescence mounting base (13). The fluorescence adjusting block (1201) is concentrically mounted with the central block (8).

8. The adjusting device for the optical path of microorganism detection according to claim 1, characterized in that: The third adjusting structure (12) further includes an adjusting nut (1202) provided between the central block (8) and the fluorescence adjusting block (1201).

9. The adjusting device for the optical path of microorganism detection according to claim 7, characterized in that: A third sliding groove (18) for the fluorescence adjusting block (1201) to slide is provided inside the central block (8). A second limiting section (19) for restricting the rotation of the fluorescence adjusting block (1201) is provided at the mating part of the fluorescence adjusting block (1201) and the third sliding groove (18). The third sliding groove (18) is provided with a taper angle at the end close to the flow cell.

10. The adjusting device for the optical path of microorganism detection according to claim 1, characterized in that: A plano-concave spherical mirror (20) is provided on the side of the flow cell (2) opposite to the fluorescence capture system (7). A fourth sliding groove (14) is provided on the central block (8) in the direction towards the plano-concave spherical mirror (20). The fourth sliding groove (14) is provided with a taper angle at the end close to the flow cell.

Images