Filtering device for preparing flow antibody
By designing a filter component composed of filter membrane and outer packaging material, using the tangential flow principle and PES material filter membrane, efficient separation and reuse of flow antibody filtration devices are achieved, solving the problem of high cost of ultrafiltration tubes and reducing the production cost of preparing flow antibodies.
Patent Information
- Application Number
- CN202422124248.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-30
- Publication Date
- 2025-07-25
- Estimated Expiration
- 2034-08-30
AI Technical Summary
In the prior art, when preparing flow antibodies, the use of ultrafiltration tubes as disposable consumables leads to high R&D and production costs, and the imported brand is expensive, making it difficult to effectively reduce costs.
A flow antibody filtration device is designed, and a filter component composed of a filter membrane and an outer packaging material is used to filter liquids using the tangential flow principle, and the components are split through fixing rods and through holes, which is easy to clean and reuse. The filter membrane material is PES for easy cleaning.
By effectively separating large and small molecules, improving the quality of finished products, and reusing them through the separation and cleaning of components, reducing costs.
Smart Images
Figure CN223144231U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of filtration and cleaning, in particular to a preparation flow antibody filtration device. Background Art
[0002] When preparing flow antibodies, after the labeling reaction is completed, it is necessary to replace the labeling buffer or separate the unbound substances in the reaction system. Currently, the ultrafiltration tube method is used to filter the liquid. The ultrafiltration tube is used as a disposable consumable, and the research and development production cost is high. To obtain an ideal separation effect and high recovery rate, currently imported brands of ultrafiltration tubes are used, and the price of a single tube is relatively high, so the cost is relatively high. Summary of the Utility Model
[0003] To overcome the above disadvantages, the purpose of the utility model is to provide a preparation flow antibody filtration device. By using a filter membrane to filter large molecular particles in the liquid, the liquid enters the packaging material from the input port, passes through the input surface of the filter membrane for filtration, and flows out from the output surface. The filtered liquid flows out of the filtration component through the output port. By using the principle of tangential flow, the separation of large and small molecules can be made more effective, thereby improving the quality of the finished product. At the same time, by using the fixing rod and through holes to install and fix the filter membrane and the outer packaging material, it is beneficial to disassemble the filtration component, thereby facilitating cleaning and achieving the purpose of repeated use and cost reduction.
[0004] To achieve the above purpose, the technical solution adopted by the utility model is: a preparation flow antibody filtration device, comprising:
[0005] A filtration component, the filtration component includes a filter membrane, an outer packaging material and a fixing rod. The outer packaging material includes a first outer packaging material and a second outer packaging material. The filter membrane is arranged between the first outer packaging material and the second outer packaging material. The first outer packaging material is provided with an input port and an output port. The filter membrane includes an input surface and an output surface. The input port communicates with the input surface, and the output port communicates with the output surface. The filter membrane and the outer packaging material are provided with fixing through holes, and the filter membrane and the outer packaging material are installed and fixed through the fixing through holes and the fixing rod.
[0006] In this technical solution, the filtration component is used to filter the liquid, the outer packaging material is used to form a closed environment to prevent the liquid from overflowing and causing waste of resources, the filter membrane is used to filter large molecular particles in the liquid. The liquid enters the packaging material from the input port, passes through the input surface of the filter membrane for filtration, and flows out from the output surface. The filtered liquid flows out of the filtration component through the output port. By using the principle of tangential flow, the separation of large and small molecules can be made more effective, thereby improving the quality of the finished product. At the same time, by using the fixing rod and through holes to install and fix the filter membrane and the outer packaging material, it is beneficial to disassemble the filtration component, thereby facilitating cleaning and achieving the purpose of repeated use and cost reduction.
[0007] In some embodiments, the second outer package is provided with a waste port, and the waste port is connected to the input surface.
[0008] In this technical solution, the waste port is used to discharge large molecular particles, facilitating the subsequent cleaning of the filter component.
[0009] In some embodiments, the preparation of the flow cytometry antibody filtration device further includes an input terminal, an output terminal, and a waste terminal. The input terminal is disposed at the input port, the output terminal is disposed at the output port, and the waste terminal is disposed at the waste port.
[0010] In this technical solution, the input terminal is used to connect other components to the input port, facilitating the introduction of liquid into the filter component through the input port. The output port terminal is used to connect other components to the output port, facilitating the export of liquid from the filter component through the output port. The waste terminal is used to connect other components to the waste port, facilitating the recycling and treatment of waste.
[0011] In some embodiments, the input port includes a first input port and a second input port, the output port includes a first output port and a second output port, the filter membrane includes a first filter membrane and a second filter membrane. The first input port conducts the input surface, the first output port conducts the output surface, the second input port is connected to the first output port, the second input port conducts the input surface, and the second output port conducts the output surface.
[0012] In this technical solution, the liquid filtered by the first filter membrane is guided from the first outlet to the second inlet for secondary filtration through the second filter membrane, further improving the quality of the finished product.
[0013] In some embodiments, the input terminal is disposed at the first input port, and the output terminal is disposed at the second output port.
[0014] In this technical solution, the unfiltered liquid is input into the filter component through the first input port, and the filtered liquid is output from the filter component through the second output port.
[0015] In some embodiments, the preparation of the flow cytometry antibody filtration device further includes a diversion tube. The diversion tube includes an input diversion tube, an output diversion tube, and a waste diversion tube. The input diversion tube is connected to the input terminal, the output diversion tube is connected to the output terminal, and the waste diversion tube is connected to the waste terminal.
[0016] In this technical solution, the diversion tube is used to conduct the input terminal and the output terminal. The input diversion tube is used to guide the liquid from the outside to the input terminal, and the output diversion tube is used to export the liquid from the output terminal.
[0017] In some embodiments, the preparation of the flow cytometry antibody filtration device further includes a driving component, and the driving component is connected to the output diversion pipe.
[0018] In this technical solution, the driving component is used to drive the liquid to flow in a specific direction, so as to achieve the filtering effect.
[0019] In some embodiments, the material of the filter membrane is PES.
[0020] In this technical solution, the PES material is beneficial to the cleaning of the filter membrane, achieving the effect of repeated use.
[0021] The beneficial effects of the present utility model are as follows: by using the filter membrane to filter large molecular particles in the liquid, the liquid enters the packaging material from the input port, passes through the input surface of the filter membrane for filtration, and flows out from the output surface. The filtered liquid flows out of the filtration component through the output port. By using the principle of tangential flow, the separation of large and small molecules can be made more effective, thereby improving the quality of the finished product. At the same time, by using the fixing rods and through holes to install and fix the filter membrane and the outer packaging material, it is beneficial to disassemble the filtration component, which is convenient for cleaning, achieving the purpose of repeated use and cost reduction; by setting the material of the filter membrane as PES, it is beneficial to the cleaning of the filter membrane, thereby achieving the effect of repeated use of the filter membrane. Description of the Drawings
[0022] Figure 1 It is a schematic diagram of the overall preparation flow cytometry antibody filtration device according to an embodiment of the present utility model;
[0023] Figure 2 It is a schematic diagram of the filtration component of the preparation flow cytometry antibody filtration device according to an embodiment of the present utility model Figure 1 ;
[0024] Figure 3 It is a schematic diagram of the filter component of the preparation flow cytometry antibody filtration device according to an embodiment of the present utility model Figure 2 ;
[0025] In the figure: 1. Filtration component; 11. First outer packaging material; 111. Input port; 1111. First input port; 1112. Second input port; 112. Output port; 1121. First output port; 1122. Second output port; 12. Second outer packaging material; 121. Waste port; 13. Filter membrane; 131. First filter membrane; 132. Second filter membrane; 14. Fixed through hole; 21. Input diversion pipe; 22. Output diversion pipe; 23. Waste diversion pipe; 3. Driving component. Detailed Embodiments
[0026] The following elaborates on the preferred embodiments of the present utility model in conjunction with the accompanying drawings, so that the advantages and features of the present utility model can be more easily understood by those skilled in the art, thereby making the protection scope of the present utility model more clearly defined.
[0027] Combined with the attached Figure 1 and the attached Figure 2 As shown, the present utility model provides a preparation flow antibody filtration device, including:
[0028] A filtration component 1, the filtration component 1 includes a filter membrane 13, an outer package material, and a fixing rod. The outer package material includes a first outer package material 11 and a second outer package material 12. The filter membrane 13 is disposed between the first outer package material 11 and the second outer package material 12. An input port 111 and an output port 112 are provided on the first outer package material 11. The filter membrane 13 includes an input surface and an output surface. The input port 111 communicates with the input surface, and the output port 112 communicates with the output surface. Fixing through holes 14 are provided on the filter membrane 13 and the outer package material. The filter membrane 13 and the outer package material are installed and fixed through the fixing through holes 14 and the fixing rod. For example, a hose can be provided, or a liquid circulation channel can be provided inside the outer package material, so that the input port 111 communicates with the input surface of the filter membrane and guides the liquid filtered by the filter membrane to the output port 112.
[0029] The filtration component 1 is used to filter the liquid. The outer package material is used to form a closed environment to prevent the liquid from overflowing and causing waste of resources. The filter membrane 13 is used to filter large molecular particles in the liquid. The liquid enters the package material through the input port 111, is filtered through the input surface of the filter membrane 13, flows out from the output surface, and the filtered liquid flows out of the filtration component 1 through the output port 112. By utilizing the principle of tangential flow, the separation of large and small molecules can be made more effective, thereby improving the quality of the finished product. At the same time, by using the fixing rod and through holes to install and fix the filter membrane 13 and the outer package material, it is beneficial to disassemble the filtration component 1, thereby facilitating cleaning and achieving the purpose of repeated use and cost reduction.
[0030] Continuing to combine with the attached Figure 2 As shown, in some embodiments, the second outer package material 12 is provided with a waste material port 121. The waste material port 121 is connected to the input surface. The waste material port 121 is used to discharge large molecular particles to facilitate subsequent cleaning of the filtration component 1.
[0031] In some embodiments, the preparation of the flow cytometry antibody filtration device further includes an input terminal, an output terminal, and a waste terminal. The input terminal is disposed at the input port 111, the output terminal is disposed at the output port 112, and the waste terminal is disposed at the waste port 121. The input terminal is used to connect other components to the input port 111, facilitating the introduction of liquid into the filtration component 1 through the input port 111. The output port 112 terminal is used to connect other components to the output port 112, facilitating the export of liquid from the filtration component 1 through the output port 112. The waste terminal is used to connect other components to the waste port 121, facilitating the recovery and treatment of waste.
[0032] Continuing to refer to the attached Figure 3 As shown, in some embodiments, the input port 111 includes a first input port 1111 and a second input port 1112. The output port 112 includes a first output port 1121 and a second output port 1122. The filter membrane 13 includes a first filter membrane 131 and a second filter membrane 132. The first input port 1111 conducts the input surface, the first output port 1121 conducts the output surface, the second input port 1112 is connected to the first output port 1121, the second input port 1112 conducts the input surface, and the second output port 1122 conducts the output surface. The liquid filtered by the first filter membrane 131 is directed from the first outlet to the second inlet and further filtered by the second filter membrane 132 to further improve the quality of the finished product.
[0033] In some embodiments, the input terminal is disposed at the first input port 1111, and the output terminal is disposed at the second output port 1122. The unfiltered liquid is input into the filtration component 1 through the first input port 1111, and the filtered liquid is output from the filtration component 1 through the second output port 1122.
[0034] Continuing to refer to Figure 1, in some embodiments, the preparation of the flow cytometry antibody filtration device further includes a diversion tube. The diversion tube includes an input diversion tube 21, an output diversion tube 22, and a waste diversion tube 23. The input diversion tube 21 is connected to the input terminal, the output diversion tube 22 is connected to the output terminal, and the waste diversion tube 23 is connected to the waste terminal. The diversion tube is used to conduct the input terminal and the output terminal. The input diversion tube 21 is used to direct the liquid from the outside to the input terminal, and the output diversion tube 22 is used to export the liquid from the output terminal.
[0035] Continuing to refer to the attached Figure 1As shown, in some embodiments, the preparation of the flow cytometry antibody filtration device further includes a driving assembly 3. The driving assembly 3 is connected to the output diversion pipe 22. The driving assembly 3 is used to drive the liquid to flow directionally, so as to achieve the filtration effect, and by providing power to the liquid in the output diversion pipe 22, it prevents the unfiltered liquid from flowing towards the waste outlet 121.
[0036] In some embodiments, the material of the filter membrane 13 is PES. The PES material is conducive to the cleaning of the filter membrane 13, achieving the effect of repeated use.
[0037] In summary, the present invention provides a preparation flow cytometry antibody filtration device. By using a filter membrane to filter macromolecular particles in the liquid, the liquid enters the packaging material from the input port, passes through the input surface of the filter membrane for filtration, and flows out from the output surface. The filtered liquid flows out of the filtration assembly through the output port. By using the principle of tangential flow, the separation of large and small molecules can be made more effective, thereby improving the quality of the finished product. At the same time, by using the fixing rod and through hole to install and fix the filter membrane and the outer packaging material, it is beneficial to disassemble the filtration assembly, thereby facilitating cleaning and achieving the purpose of repeated use and cost reduction; by setting the material of the filter membrane as PES, it is conducive to the cleaning of the filter membrane, thereby achieving the effect of repeated use of the filter membrane.
[0038] The above embodiments are only for illustrating the technical concept and features of the present invention, and their purpose is to enable those who are familiar with this technology to understand the content of the present invention and implement it. It cannot be used to limit the protection scope of the present invention. Any equivalent changes or modifications made according to the spirit of the present invention should be covered within the protection scope of the present invention.
Claims
1. A preparation method of a flow cytometry antibody filtration device, characterized in that, Comprising: A filtering component, the filtering component includes a filter membrane, an outer wrapper, and a fixing rod. The outer wrapper includes a first outer wrapper and a second outer wrapper. The filter membrane is disposed between the first outer wrapper and the second outer wrapper. An input port and an output port are provided on the first outer wrapper. The filter membrane includes an input surface and an output surface. The input port communicates with the input surface, and the output port communicates with the output surface. Fixing through holes are provided on the filter membrane and the outer wrapper. The filter membrane and the outer wrapper are installed and fixed through the fixing through holes and the fixing rod.
2. The preparation method of the flow cytometry antibody filtration device according to claim 1, wherein The second outer wrapper is provided with a waste port, and the waste port is connected to the input surface.
3. The preparation method of the flow cytometry antibody filtering device according to claim 2, wherein, It further includes an input terminal, an output terminal, and a waste terminal. The input terminal is disposed at the input port, the output terminal is disposed at the output port, and the waste terminal is disposed at the waste port.
4. The preparation method of the flow cytometry antibody filtration device according to claim 3, wherein, The input port includes a first input port and a second input port. The output port includes a first output port and a second output port. The filter membrane includes a first filter membrane and a second filter membrane. The first input port communicates with the input surface, the first output port communicates with the output surface, the second input port is connected to the first output port, the second input port communicates with the input surface, and the second output port communicates with the output surface.
5. The preparation method of the flow cytometry antibody filtration device according to claim 4, wherein The input terminal is disposed at the first input port, and the output terminal is disposed at the second output port.
6. The flow cytometry antibody filtration device according to claim 3, wherein It further includes a diversion tube. The diversion tube includes an input diversion tube, an output diversion tube, and a waste diversion tube. The input diversion tube is connected to the input terminal, the output diversion tube is connected to the output terminal, and the waste diversion tube is connected to the waste terminal.
7. The preparation method of the flow cytometry antibody filtration device according to claim 6, wherein It further includes a driving component, and the driving component is connected to the output diversion tube.
8. The preparation method of the flow cytometry antibody filtration device according to claim 1, wherein The material of the filter membrane is PES.